Bionic nanobamboo based on skeleton plug-in center rigidization series assembly and application thereof in circulating tumor cell separation

The branching and sprouting DNA-inspired nanobamboo constructed using CRSA technology solves the problems of low capture efficiency and poor stability in CTC separation technology, achieving efficient and specific CTC separation and good cell viability maintenance.

CN122326534APending Publication Date: 2026-07-03WENZHOU MEDICAL UNIV
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Patent Information

Application Number
CN202610498618.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-15
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Existing CTC separation technologies suffer from problems such as low capture efficiency, insufficient specificity, and poor stability of nanocarriers, making it difficult to achieve efficient and specific separation of circulating tumor cells.

Method used

A branching, sprouting DNA-inspired bionic bamboo nanoparticle was constructed using central rigid tandem assembly (CRSA) technology. By mimicking the structural characteristics of natural bamboo and combining the programmability of DNA nanotechnology with RCA technology, efficient and specific separation of CTCs was achieved.

Benefits of technology

It achieves highly efficient capture and separation of CTCs, with a separation efficiency improved by 68.2 times and a purity of 75.6%, while maintaining cell viability, making it suitable for subsequent molecular biology analysis.

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Abstract

This invention discloses a biomimetic nanobamboo based on centrally rigid tandem assembly (CRSA) technology and its application in the isolation of circulating tumor cells (CTCs). This biomimetic nanobamboo uses rigid triangular structural units with a Y-shaped framework as lateral partitions, and is longitudinally tandemly linked by rolling circle amplification products (sRCAp), cross-linked with linker molecules to form a super-large, highly rigid nanobamboo structure. Surface modification with targeted aptamers acts as "branches," and biotin modification is used for magnetic separation. This invention achieves highly efficient assembly of the nanobamboo using CRSA technology, with an assembly efficiency of 90%. Its structural rigidity is 33 times higher than that of traditional DNA nanotubes, and its resistance to nuclease degradation is enhanced by 30 times. Utilizing the multivalent aptamer binding effect and magnetic separation technology, this biomimetic nanobamboo can efficiently separate rare CTCs from whole blood samples, achieving a separation efficiency of 68.2 times and a purity of 75.6%, while maintaining good activity of the separated CTCs. This invention provides a novel tool for the precise isolation of CTCs and has significant application prospects in tumor metastasis early warning and precision medicine.
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