Pharmaceutical compositions, methods of making and using the same

Abstract

The application provides a kind of pharmaceutical composition and its preparation method and application, and the active ingredient of pharmaceutical composition is composed of camptothecin, 9-methoxy camptothecin and camptothecin glucoside, and the preparation method comprises: the plant part of Maybium ferrugineum is cleaned, and the pretreated raw material is obtained by drying to change mature and crushing at the first preset temperature;The pretreated raw material is extracted by reflux extraction with the first extraction solvent for the first extraction times, and each extraction is carried out for the first preset time, and the extract obtained by each extraction is combined and concentrated to obtain extract;The extract is dispersed with water, sequentially extracted with petroleum ether, ethyl acetate, and water-saturated n-butanol is extracted to obtain total glycoside;Total glycoside is purified to collect the fraction containing camptothecin, 9-methoxy camptothecin and camptothecin glucoside, and concentrated and crystallized to obtain the pharmaceutical composition.The pharmaceutical composition of the application has significant synergistic antitumor effect, and provides an efficient and low-toxicity treatment option for clinical use.

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