A white goat feed containing zearalenone-degrading enzyme and a preparation method and application thereof

Abstract

The application discloses a white goat feed containing zearalenone degrading enzyme, and comprises a basic daily ration and zearalenone degrading enzyme, wherein the addition amount of the zearalenone degrading enzyme is 0.01%-0.10% of the total weight of the feed. The zearalenone in the feed is subjected to enzymatic degradation reaction in the digestive tract of the animal by adding the zearalenone degrading enzyme in the basic daily ration of the white goat, so that the residual amount of the zearalenone in the gastrointestinal tract is reduced, the estrogen-like toxic effect of the zearalenone on the female white goat is reduced, and problems such as vulva swelling, abnormal reproductive organs and abnormal follicular development of the ovary caused by zearalenone pollution are effectively relieved.

Description

Technical Field

[0001] This invention relates to the field of biology, and in particular to a white goat feed containing zearalenone-degrading enzyme, its preparation method, and its application. Background Technology

[0002] Zearalenone (ZEN) is a fungal toxin produced by Fusarium fungi and is widely found in grains such as corn, wheat, and barley, as well as their byproducts. Because white goat feed typically contains a high proportion of corn and other grains, zearalenone contamination is common in white goat farming. Zearalenone has significant estrogen-like effects; after ingestion, it easily disrupts the endocrine system of animals, particularly affecting the reproductive system of female white goats.

[0003] Studies have shown that white goats are highly sensitive to zearalenone. When the zearalenone content in feed reaches a certain level, female white goats are prone to vulvar swelling, abnormal development of reproductive organs, abnormal ovarian follicle development, and decreased reproductive performance. It can also lead to reduced feed intake, decreased growth performance, and weakened antioxidant capacity, resulting in significant economic losses in white goat farming. Therefore, reducing the toxic effects of zearalenone in feed on white goats has always been an important research direction in the field of feed safety.

[0004] Currently, various methods are used in actual production to reduce the harm of zearalenone, such as selecting low-toxicity raw materials, using physical adsorbents, or adding chemical detoxifying agents. However, physical adsorbents have limited adsorption capacity for zearalenone and may also adsorb nutrients in the feed, thus affecting feed utilization. While some chemical detoxification methods can reduce toxin content, they may pose safety issues or have potential adverse effects on animals.

[0005] In recent years, the biodegradation of mycotoxins using microorganisms or enzyme preparations has attracted increasing attention. Related studies have shown that enzymes produced by some microorganisms can catalyze the structural transformation of zearalenone molecules, degrading them into less toxic or non-toxic metabolites, thereby reducing their harm to animals. However, in practical applications, the stability of enzyme preparations during feed processing and the maintenance of their activity in the animal's gastrointestinal environment still present certain challenges, affecting their degradation efficiency and application effectiveness. Summary of the Invention

[0006] The present invention relates to a white goat feed containing zearalenone degrading enzyme, its preparation method, and its application, which are used to solve related technical problems in the background art.

[0007] The technical solution provided by the present invention is as follows: a white goat feed containing zearalenone degrading enzyme, comprising: a basic diet and zearalenone degrading enzyme, wherein the amount of zearalenone degrading enzyme added is 0.01% to 0.10% of the total weight of the feed.

[0008] In one embodiment, the amount of zearalenone-degrading enzyme added is 0.05%.

[0009] In one embodiment, the basic diet comprises the following ingredients by weight percentage: 50%–65% corn, 10%–18% soybean meal, 8%–15% puffed soybeans, 1%–5% fish meal, 1%–4% fat meal, 0.5%–3% mineral additives, and 0.01%–0.5% vitamin additives.

[0010] In one embodiment, the zearalenone-degrading enzyme is an extracellular enzyme produced by Bacillus.

[0011] In one embodiment, the Bacillus is selected from one or more of the following: Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus.

[0012] In one embodiment, the zearalenone-degrading enzyme is a coated enzyme preparation.

[0013] In one embodiment, the coating layer is one or more of the following materials: lipid materials, starch derivatives, alginate, and chitosan.

[0014] A method for preparing feed for white goats containing zearalenone degrading enzyme involves mixing the zearalenone degrading enzyme evenly with the basal diet to achieve a final concentration of 0.01% to 0.10% of the degrading enzyme in the feed.

[0015] Application of a white goat feed containing zearalenone-degrading enzyme in alleviating zearalenone toxicity in female white goats.

[0016] In one embodiment, the toxicity includes one or more of the following: decreased growth performance, vulvar redness and swelling, increased reproductive organ index, increased serum estradiol, abnormal ovarian follicle development, increased gastrointestinal toxin residues, and decreased antioxidant capacity.

[0017] Compared with the prior art, the beneficial effects of the present invention are: (1) This invention adds zearalenone-degrading enzyme to the basic diet of white goats, so that zearalenone in the feed undergoes enzymatic degradation in the animal's digestive tract, thereby reducing its residual amount in the gastrointestinal tract, reducing the estrogen-like toxicity of zearalenone to female white goats, and effectively alleviating problems such as vulvar redness and swelling, abnormal reproductive organs and abnormal ovarian follicle development caused by zearalenone pollution.

[0018] (2) By adding zearalenone degrading enzyme to the feed, the present invention can reduce the interference of zearalenone on the metabolism of the animal body, reduce its adverse effects on feed intake, weight gain and endocrine system, thereby increasing the average daily weight gain and feed intake of female white goats and reducing the feed conversion ratio, which is beneficial to maintaining the normal growth performance and reproductive function of female white goats.

[0019] (3) The present invention prepares zearalenone degrading enzyme as a coated enzyme preparation, which makes the enzyme preparation have better heat resistance and stability during feed processing. At the same time, it can avoid gastric acid inactivation in the animal gastrointestinal environment, thereby improving the release efficiency and toxin degradation ability of the enzyme in the intestine, and further enhancing the degradation effect of zearalenone. Attached Figure Description

[0020] Figure 1 This is a schematic diagram illustrating the effect of ZEN and its degrading enzyme on the vulva area of ​​a female white goat. Figure 2 This is a schematic diagram illustrating the effect of ZEN and its degrading enzymes on hormone levels in female white goats. Figure 3 This is a schematic diagram illustrating the effects of ZEN and its degrading enzymes on the morphology of the ovaries and uterus of female white goats. Detailed Implementation

[0021] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make several changes and improvements without departing from the concept of the present invention. These all fall within the protection scope of the present invention.

[0022] like Figure 1-3 As shown, the present invention provides a white goat feed containing zearalenone-degrading enzyme. By adding a certain amount of zearalenone-degrading enzyme to the basal diet, zearalenone (ZEN) that may be present in the feed undergoes an enzymatic degradation reaction in the digestive tract of white goats, thereby reducing the toxic effects of zearalenone on the growth performance and reproductive system of female white goats.

[0023] The amount of zearalenone-degrading enzyme added is 0.01% to 0.10% of the total weight of the feed, preferably 0.05%.

[0024] The basic diet may include the following ingredients by weight percentage: corn 50%–65%, soybean meal 10%–18%, puffed soybeans 8%–15%, fish meal 1%–5%, fat meal 1%–4%, mineral additives 0.5%–3%, and vitamin additives 0.01%–0.5%.

[0025] The zearalenone-degrading enzyme can be an extracellular enzyme produced by Bacillus, preferably one or more of Bacillus subtilis, Bacillus licheniformis, or Bacillus pumilus.

[0026] In some embodiments, the zearalenone-degrading enzyme can also be prepared as a coated enzyme preparation to improve its stability in feed processing and the animal digestive tract environment. The coating material can be one or more of lipid materials, starch derivatives, alginate, or chitosan.

[0027] Through the above technical solution, when white goats ingest this feed, the zearalenone degrading enzyme can catalyze the structural transformation of zearalenone molecules in the digestive tract, degrading them into metabolites with lower or no toxicity, thereby reducing their residue in the stomach and intestines and improving the growth performance and reproductive health of female white goats.

[0028] Example 1 This embodiment provides a white goat feed containing zearalenone-degrading enzyme, the composition of which includes a basal diet and zearalenone-degrading enzyme.

[0029] The basic daily ration comprises the following ingredients by weight percentage: 60% corn, 15% soybean meal, 10% extruded soybeans, 3% fish meal, 2% fat meal, 2% mineral additives, 0.1% vitamin additives, and the remainder being conventional feed supplements. Among these: Mineral additives include one or more of calcium carbonate, dicalcium phosphate, salt, and trace element premixes.

[0030] Vitamin supplements include one or more of vitamin A, vitamin D3, vitamin E, vitamin K3, and B vitamins.

[0031] The zearalenone-degrading enzyme described in this embodiment is an extracellular enzyme produced by Bacillus.

[0032] In this embodiment, the Bacillus is selected from one or more of the following: Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus. Preferably, the zearalenone-degrading enzyme preparation produced by Bacillus subtilis fermentation is used in this embodiment.

[0033] In this embodiment, the amount of zearalenone-degrading enzyme added to the feed is 0.05% (based on the total weight of the feed).

[0034] The feed for white goats containing zearalenone-degrading enzyme is prepared through the following steps: Step 1: Weigh the raw materials according to the above formula, and grind the corn, soybean meal and puffed soybeans to make the particle size 0.5-1.5mm.

[0035] Step 2: Add the pulverized raw materials to a horizontal mixer for initial mixing, which takes 5 to 10 minutes.

[0036] Step 3: Add 0.05% of zearalenone degrading enzyme to the mixer and mix it with the basal diet for 5-8 minutes.

[0037] Step 4: Pelletize the well-mixed feed, controlling the pelleting temperature at 60-75℃.

[0038] Step 5: After pelleting, the product is cooled and dried to obtain white goat feed containing zearalenone-degrading enzyme.

[0039] The enzyme preparations in the obtained feed are evenly distributed and can effectively degrade zearalenone in the digestive tract of white goats.

[0040] Example 2 To improve the stability of enzyme preparations during feed processing and their acid resistance in the animal gastrointestinal tract, this embodiment provides a white goat feed containing coated zearalenone degrading enzyme.

[0041] The basic diet consists of the following ingredients by weight percentage: corn 58%, soybean meal 16%, extruded soybeans 12%, fish meal 3%, fat meal 2%, mineral additives 2%, vitamin additives 0.1%, and the remainder is feed supplements.

[0042] The zearalenone-degrading enzyme is coated using microencapsulation technology.

[0043] The coating material is selected from one or more of the following: lipid materials, starch derivatives, alginate, chitosan.

[0044] In this embodiment, a sodium alginate-chitosan composite coating system is preferred.

[0045] The preparation steps are as follows: Step 1: Mix the zearalenone degrading enzyme solution with the sodium alginate solution to form a homogeneous solution.

[0046] Step 2: The above mixed solution is added dropwise to calcium chloride solution to carry out ionic cross-linking and form primary microcapsules.

[0047] Step 3: The formed microcapsules are further coated in a chitosan solution to form a composite coating layer.

[0048] Step 4: After drying, the coated zearalenone-degrading enzyme preparation is obtained.

[0049] Add the coated zearalenone degrading enzyme to the basal diet at a ratio of 0.05%, and mix it with a mixer for 8-10 minutes to ensure that it is evenly distributed in the feed.

[0050] The product is then granulated, cooled, and packaged to obtain white goat feed containing coated zearalenone-degrading enzymes.

[0051] Because enzyme preparations have an outer coating layer, their resistance to high temperatures and acids can be effectively improved, thus ensuring their stability during feed processing and in the animal's gastrointestinal environment.

[0052] Experimental Groups: This patent selected 64 healthy, 42-day-old female white goats of similar weight (9.82±0.78kg) and randomly divided them into 4 treatment groups, with 16 goats in each group, ensuring that the initial weight difference between the groups was not significant (P>0.05). The control group of female white goats was fed a basal diet (BD) that met the nutritional requirements of NRC (2012). The other four treatment groups were fed BD supplemented with 0.4mg / kg ZEN (simulating ZEN in feed); 0.4mg / kg ZEN + 0.05% ZEN degrading enzyme (Example 1); and 0.05% ZEN degrading enzyme coated product (Example 2), respectively. The experimental design and grouping are shown in Table 1.

[0053] Before the experiment began, the goat pens were thoroughly cleaned and disinfected. During the experiment, the goat pens were disinfected once a week. Infrared heat lamps were installed inside the pens. During the first week, the temperature inside the experimental cages was maintained at around 30℃, and during the second week, the ambient temperature inside the goat pens was maintained at 26-28℃. For four weeks, the female goats had free access to food and water, and their feeding, management, and immunization were carried out according to standard procedures.

[0054] Table 1 Experimental Groups Measurement indicators: 1. Growth performance Record the daily feed intake of the female white goats in each pen after the start of the experiment. Record the weight of the female white goats on the 1st, 14th and 28th days of the experiment, and calculate their average feed intake (ADFI), average daily weight gain (ADG) and feed conversion ratio (F / G).

[0055] 2. Observation, measurement and calculation of the vulva After the experiment began, the swelling and redness of the vulva of each white goat calf were carefully observed and recorded daily. On days 1, 14, and 28 of the experiment, the length (a) and width (b) of the vulva were measured using calipers, and the area of ​​the vulva was calculated. The area of ​​the vulva of the white goat calf was calculated using a formula similar to that of a rhombus, S=(a×b) / 2. By comparing the vulva areas, the swelling and recovery effects of the vulva of the white goats calf in each group were determined.

[0056] 3. Analysis of organ health status On day 28 of the experiment, all female white goats were weighed after fasting, and five goats from each treatment were slaughtered. Blood was collected from each female white goat for serum biochemistry and hormone level testing; liver, vagina, uterine horn, and ovary were collected and weighed, and the relative organ weights were calculated. Then, appropriately sized tissue blocks were cut and fixed in 4% paraformaldehyde for subsequent morphological examination. The remaining parts were shredded and quickly placed in liquid nitrogen for subsequent testing.

[0057] 4. Serum biochemistry and sex hormone level measurement Blood was centrifuged at 3000 rpm for 15 min to separate serum, which was then stored in separate containers at -20°C. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and total bilirubin (TBIL) were measured. Serum hormone levels of growth hormone (GH), luteinizing hormone (LH), follicle-stimulating hormone (FSH), and estradiol (E2) were determined using ELISA.

[0058] 5. Histological evaluation of reproductive organs Uterine horn and ovarian samples were fixed in 4% paraformaldehyde for 48 hours, then dehydrated, cleared, paraffin-embedded, embedded, and sectioned. They were then stained with hematoxylin and eosin (H&E), and the sections were observed and images were acquired under an optical microscope to analyze the morphological changes of the organs.

[0059] 6. Detection of ZEN degradation products in the digestive tract and blood After slaughter, blood, gastric juice, and chyme from the duodenum, jejunum, and ileum of the female white goats were collected for the detection of ZEN degradation products. This part was sent to Omnicom for testing (Wang et al., J. Agric. Food Chem. 2022).

[0060] 7. Determination of antioxidant indicators in reproductive organs Antioxidant-related indicators such as T-AOC, MDA, SOD, and GPX in the uterus and ovaries were determined using a colorimetric method. Protein concentrations were determined using the BCA method.

[0061] 8. Statistical Analysis All experimental data were analyzed for differences using the student t-test. The results are expressed as mean ± standard error, and P < 0.05 was considered statistically significant.

[0062] Experimental results and analysis: 1. Growth performance As shown in Table 2, compared with the CON group, the ZEN group significantly reduced (P<0.05) the body weight gain (20.2%-25.4%) and feed intake (12.6%-20.5%) of female white goats on days 1-14, 15-28, and 1-28, while increasing the feed conversion ratio (6.1%-9.0%). Furthermore, compared with the CON group, there were no significant differences (P>0.05) in the effects of Example 1 and Example 2 on the body weight gain, feed intake, and feed conversion ratio of female white goats on days 1-14, 15-28, and 1-28. Moreover, compared with the ZEN group, both Example 1 and Example 2 significantly increased (P<0.05) the daily weight gain (27.6%-53.5%) and feed intake (15.4%-32.0%) of female white goats on days 15-28 and 1-28.

[0063] Table 2. Effects of ZEN and its degrading enzymes on the growth performance of female white goats. Note: Results are expressed as mean ± standard deviation (n=16). Different lowercase letters in the same row indicate significant differences (P<0.05).

[0064] 2. Area of ​​the vulva Depend on Figure 1 It can be seen that, compared with the CON group, the ZEN group significantly increased ( P <0.05) Vulvar area of ​​female white goats on days 14 and 28 (15.7%-46.5%). However, Examples 1 and 2 can alleviate the vulvar area induced by ZEN on day 14 of female white goats.

[0065] Figure 1 Note: Vulvar area of ​​female white goats on day 14 (A) and day 28 (B) fed with different diets; n=16.

[0066] 3. Relative weight of organs As shown in Table 3, compared with the CON group, the ZEN group, ZEN+Z1 group and ZEN+Z2 group had no significant difference in the effect on the relative weight of the liver of the female white goats (P>0.05), and all of them significantly increased (P<0.05) the relative weight of the vagina (166%-275%) and uterine horn (118%-143%) of the female white goats.

[0067] Table 3. Effects of ZEN and its degrading enzymes on the relative weight of organs in female white goats. Note: Results are expressed as mean ± standard deviation (n=5). Different lowercase letters in the same row indicate significant differences (P<0.05).

[0068] 4. Serum biochemistry and hormone levels As shown in Table 4, compared with the CON group, the ZEN group only significantly increased (P<0.05) the AST activity in the serum of female white goats (59.5%), while Examples 1 and 2 could alleviate the ZEN-induced changes in AST. In addition, compared with the CON group, ZEN did not affect the levels of ALT, ALP, and TBIL in serum (P>0.05).

[0069] Table 4. Effects of ZEN and its degrading enzymes on serum biochemical parameters of female white goats. Note: Results are expressed as mean ± standard deviation (n=5). Different lowercase letters in the same row indicate significant differences (P<0.05).

[0070] Depend on Figure 2 It was found that, compared with the CON group, the ZEN group significantly increased (P<0.05) the estradiol (E2) content in the serum of female white goats (8.6%), while there were no significant differences between the ZEN+Z1 group (Example 1) and the ZEN+Z2 group (Example 2) (P>0.05). Meanwhile, compared with the CON group, there were no significant changes in the serum levels of luteinizing hormone (LH), growth hormone (GH), and follicle-stimulating hormone (FSH) in any group (P>0.05).

[0071] 5. Histopathological analysis Depend on Figure 3 It was found that, compared with CON, some primordial follicles in the ZEN group showed abnormal morphology, and the number of atretic follicles increased. Follicles in the ZEN+Z1 and ZEN+Z2 groups developed faster, and the volume of primary follicles was significantly larger. In addition, no obvious pathological changes were observed in the lamina propria and basal layer of the uterine mucosa in any group.

[0072] Figure 3 Note: Arrows represent primordial follicles with abnormal morphology; triangles represent primordial follicles that have undergone atresia; circles represent primary follicles.

[0073] 6. Analysis of ZEN degradation products in the digestive tract As shown in Table 5, compared with CON, the ZEN group significantly increased (P<0.05) the ZEN content in the stomach and duodenal contents of the female white goat. The ZEN+Z1 group (Example 1) and the ZEN+Z2 group (Example 2) reduced the ZEN content in the stomach and duodenal contents of the female white goat (P<0.05).

[0074] Table 5. Effects of ZEN and its degrading enzymes on ZEN content in the digestive tract of female white goats. Note: Results are expressed as mean ± standard deviation (n=5). Different lowercase letters in the same row indicate significant differences (P<0.05).

[0075] 7. Determination of antioxidant indicators in reproductive organs As shown in Table 6, compared with CON, the ZEN group decreased (P<0.05) the T-AOC content in the uterus, and the ZEN+Z1 group (Example 1) and the ZEN+Z2 group (Example 2) alleviated these changes. Furthermore, compared with the ZEN group, the ZEN+Z2 group decreased (P<0.05) the MDA content in the uterus. Compared with CON, the ZEN group increased (P<0.05) the T-AOC content in the ovaries, and the ZEN+Z2 group alleviated these changes. Additionally, compared with the ZEN group, the ZEN+Z2 group decreased (P<0.05) the MDA content in the ovaries.

[0076] Table 6. Effects of ZEN and its degrading enzymes on antioxidant indices of female white goats. Note: Results are expressed as mean ± standard deviation (n=5). Different lowercase letters in the same row indicate significant differences (P<0.05).

[0077] In summary: 1) Compared with the control group, the addition of 0.4 mg / kg ZEN to the basal diet reduced the growth performance of female white goats, while the addition of 0.05% zearalenone degrading enzyme and coated zearalenone degrading enzyme to the basal diet could effectively alleviate the harm of ZEN to the growth performance of female white goats.

[0078] 2) Compared with the control group, the addition of ZEN to the basal diet induced vulvar swelling, increased vaginal and uterine organ indices, serum AST activity, and estradiol levels in female white goats on days 14 and 28. It also induced abnormal morphology in some primordial follicles and an increase in the number of atretic follicles. However, the addition of zearalenone-degrading enzyme and coated zearalenone-degrading enzyme to the basal diet effectively alleviated the ZEN-induced changes in vulvar swelling, serum AST, and estradiol levels in female white goats on day 14, and resulted in faster follicle development and a significant increase in the volume of primary follicles.

[0079] 3) Compared with the control group, the addition of ZEN to the diet increased the ZEN content in the stomach and duodenal contents of the female white goats, while the addition of zearalenone degrading enzyme and coated zearalenone degrading enzyme to the basal diet reduced the ZEN content in the stomach and duodenal contents of the female white goats.

[0080] 4) Compared with the control group, dietary supplementation with ZEN induced a decrease in T-AOC in the uterus and promoted an increase in T-AOC in the ovary. Supplementation with zearalenone-degrading enzymes and coated zearalenone-degrading enzymes in the basal diet effectively alleviated these ZEN-induced changes. Furthermore, compared with the ZEN group, supplementation with coated zearalenone-degrading enzymes in the basal diet reduced MDA content in the uterus and ovary of female white goats.

[0081] In this application, the terms "upper," "lower," "left," "right," "front," "rear," "top," "bottom," "inner," "outer," "middle," "vertical," "horizontal," "lateral," and "longitudinal" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are primarily for the purpose of better describing the invention and its embodiments, and are not intended to limit the indicated device, element, or component to having a specific orientation, or to be constructed and operated in a specific orientation.

[0082] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A white goat feed containing a zearalenone-degrading enzyme, characterized in that, include: The basal diet and zearalenone-degrading enzyme, wherein the amount of zearalenone-degrading enzyme added is 0.01% to 0.10% of the total feed weight.

2. The white goat feed containing a zearalenone-degrading enzyme according to claim 1, characterized by, The amount of zearalenone-degrading enzyme added is 0.05%.

3. The white goat feed containing a zearalenone-degrading enzyme according to claim 1, characterized by, The basic diet comprises the following ingredients by weight percentage: corn 50%–65%, soybean meal 10%–18%, puffed soybeans 8%–15%, fish meal 1%–5%, fat meal 1%–4%, mineral additives 0.5%–3%, and vitamin additives 0.01%–0.5%.

4. The white goat feed containing a zearalenone-degrading enzyme according to claim 1, characterized by, The zearalenone-degrading enzyme is an extracellular enzyme produced by Bacillus.

5. A white goat feed containing a zearalenone-degrading enzyme according to claim 4, characterized in that, The Bacillus species are selected from one or more of the following: Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus.

6. The white goat feed containing a zearalenone-degrading enzyme according to claim 1, characterized by, The zearalenone-degrading enzyme is a coated enzyme preparation.

7. A white goat feed containing a zearalenone-degrading enzyme according to claim 6, characterized in that, The coating layer is one or more of the following materials: lipid materials, starch derivatives, alginate, and chitosan.

8. A method for preparing white goat feed containing zearalenone-degrading enzyme as described in any one of claims 1-7, characterized in that, Mix the zearalenone-degrading enzyme with the basal diet evenly to achieve a final concentration of 0.01% to 0.10% in the feed.

9. The use of a white goat feed containing zearalenone-degrading enzyme as described in any one of claims 1-7 in alleviating zearalenone toxicity in female white goats.

10. The white goat feed containing zearalenone-degrading enzyme as described in claim 9, characterized in that, The toxicity includes one or more of the following: decreased growth performance, vulvar redness and swelling, increased reproductive organ index, increased serum estradiol, abnormal ovarian follicle development, increased gastrointestinal toxin residues, and decreased antioxidant capacity.

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