Stem cell incubator with detection function and method of use thereof

Abstract

This invention relates to the field of stem cell culture technology, and particularly to a stem cell culture box with detection function and its usage method. The box includes a body, a base plate, and a cover plate. An annular observation window is provided on the cover plate, and a detection mechanism for observing stem cells is also provided on the cover plate. By positioning the movement trajectory of the sample carrier, liquid level fluctuations caused by tilting of the culture bottle during ascent are effectively avoided. Combined with the pressure regulation function controlled by the micro-flow one-way valve of the telescopic damping airbag, stable and uniform lifting and lowering of the sample carrier component is achieved. With the help of annular scanning by an electron microscope, multiple batches of samples can be tested without manual intervention. This effectively avoids the acceleration impact caused by traditional sample transfer methods, ensuring the stability of the stem cell suspension and effectively preventing the impact of mechanical vibration on stem cell growth, thereby effectively improving the accuracy and repeatability of stem cell detection data.

Description

Technical Field

[0001] This invention belongs to the field of stem cell culture technology, and specifically relates to a stem cell culture box with detection function and its usage method. Background Technology

[0002] Currently, stem cell culture technology is widely used in biomedical research, drug development, and regenerative medicine. Traditional stem cell culture chambers mainly provide a controlled environment with constant temperature, humidity, and CO2 concentration, but lack automated monitoring functions. Frequent opening of the chamber for sampling and observation can easily lead to fluctuations in the culture environment, affecting cell growth stability. Furthermore, existing culture chambers often use simple lifting or rotating structures for sample transport, lacking buffering mechanisms. This makes cells susceptible to mechanical vibration or impact during movement, causing cell damage and affecting the accuracy of experimental results.

[0003] A search revealed that a patent document with publication number CN118956585B and publication date of January 7, 2025, discloses a stem cell culture box with detection function, relating to the field of cell culture equipment technology. The box includes: a box body, a rotating assembly, a placement assembly, a pushing assembly, a linkage assembly, and a conveyor belt. The rotating assembly is located inside the box body, the placement assembly is located beside the rotating assembly, the pushing assembly is flush with the bottom of the placement assembly, and the linkage assembly is connected to the output end of the placement assembly. The two ends of the conveyor belt are located beside the rotating assembly and the placement assembly, respectively. When the placement assembly moves the placement platform, it also drives the opening and closing structure in the placement platform through the linkage assembly, thereby controlling the opening and closing of the bottom of the placement platform. This invention, through the rotating assembly, allows for comparative observation of multiple culture bottles, ensuring the effectiveness of the detection. Furthermore, by cooperating with the other components, it enables the replacement and movement of culture bottles, avoiding the accidental dangers of manual movement, ensuring a safe culture environment, and facilitating related work.

[0004] However, the device still has the following drawbacks: although it can replace and move culture bottles, when the culture bottles are transferred between the conveyor belt and the placement platform, they rely solely on gravity to slide down. This can easily cause the cell suspension to vibrate or the culture bottles to break due to collisions. Furthermore, it is not easy to locate the falling culture bottles, which can easily cause them to shift during the fall and affect the observation of stem cells. Summary of the Invention

[0005] To address the above problems, the present invention provides a stem cell culture box with detection function and its usage method, including a box body, a bottom plate and a cover plate, wherein an annular observation window is provided on the cover plate and a detection mechanism for observing stem cells is provided on the cover plate; The box is equipped with a conveying mechanism, which includes a second electric turntable and several sets of limiting shafts. The second electric turntable is rotatably connected to the center of the top of the bottom plate. A rotating shaft is fixedly connected to the center of the second electric turntable. A spiral conveying plate for conveying stem cell samples is provided on the rotating shaft. An arc-shaped abutment block is provided at the top of the rotating shaft, and the arc-shaped abutment block is in contact with the top of the spiral conveying plate. Each of the aforementioned limiting shafts is slidably connected to a sample loading component for loading stem cell samples. Several sets of limiting shafts are fitted with telescopic damping airbags for buffering and limiting the speed of the sample carrier assembly, and several sets of telescopic damping airbags are set between the corresponding set of sample carrier assembly and the base plate. The telescopic damping airbag is equipped with an air inlet pipe and an air outlet pipe, and both the air inlet pipe and the air outlet pipe are equipped with a one-way valve with adjustable flow rate.

[0006] Furthermore, the box body is fixedly connected to the bottom plate, and the box body is fixedly connected to the cover plate. The side wall of the box body is provided with several sets of first pick-up and put-out slots, and the several sets of first pick-up and put-out slots are arranged in a circular array with the central axis of the box body as the center. The box body is provided with a constant temperature and humidity control component, which includes a controller, a temperature adjustment device and a humidity adjustment device.

[0007] Furthermore, the detection mechanism includes a first electric turntable, a light-shielding plate is installed at the center of the bottom end of the first electric turntable, the top of the light-shielding plate is covered with a nanofiber cleaning cloth, a viewing groove is opened on the light-shielding plate, a movable frame is installed at the center of the top end of the first electric turntable, an electron microscope is installed on the movable frame, a support shaft is fixedly connected to the bottom of the other end of the movable frame, a roller is provided at the bottom end of the support shaft, and an annular groove is opened on the cover plate, the annular groove is movably fitted with the roller.

[0008] Furthermore, the sample carrier assembly includes an incomplete gear, the bottom end of which is fixedly connected to the top end of the telescopic damping airbag, and a limiting hole is formed at the center of the incomplete gear, which is movably fitted with a corresponding set of limiting shafts.

[0009] Furthermore, a sample carrier box is fixedly connected to the incomplete gear, a buffer pad is provided inside the sample carrier box, a sample carrier slot is opened on the buffer pad, a culture bottle is placed in the sample carrier slot, a stem cell sample is contained in the culture bottle, the culture bottle is made of transparent material, and a transparent magnetic cap is provided on the sample carrier box.

[0010] Furthermore, the sample container is provided with an abutting mechanism, which includes an abutting block. The abutting block movably abuts against the upper surface of the rotating spiral conveyor plate. A sliding shaft is fixedly connected to one side of the abutting block. A first receiving groove is provided on the sample container. The first receiving groove is movably fitted with the abutting block. A first spring is provided between the inner wall of the first receiving groove and the abutting block. The first spring is sleeved on the sliding shaft. A sliding groove is provided on the sample container. The sliding groove is movably fitted with the sliding shaft.

[0011] Furthermore, the side wall of the sliding shaft is provided with a snap-fit ​​groove, and the sample container is provided with an installation groove and a second receiving groove. The sample container is provided with a snap-fit ​​part, which includes a cam and a wedge-shaped snap-fit ​​block. The cam is rotatably connected to the inner wall of the installation groove. A micro motor is also installed in the installation groove. The output end of the micro motor is connected to the center of the cam. The wedge-shaped snap-fit ​​block is movably snapped into the snap-fit ​​groove. The wedge-shaped snap-fit ​​block is movably fitted into the second receiving groove. A linkage shaft is fixedly connected to the bottom end of the wedge-shaped snap-fit ​​block. A second spring is provided between the wedge-shaped snap-fit ​​block and the inner wall of the second receiving groove. The second spring is sleeved on the linkage shaft. The bottom end of the linkage shaft extends into the installation groove and is fixedly connected to an extension plate. The other end of the extension plate movably abuts against the cam.

[0012] Furthermore, an annular baffle plate is slidably connected to the bottom of the inner side of the housing, and several sets of racks are fixedly connected to the top of the annular baffle plate. A hollow ring is fixedly connected to the outer wall of the housing, and several sets of cylindrical gears are rotatably connected inside the hollow ring. One end of each set of cylindrical gears passes through the hollow ring and extends into the interior of the housing, and each set of cylindrical gears meshes with a corresponding set of racks. An annular rack is rotatably connected to the bottom of the inner wall of the hollow ring, and the annular rack meshes with several sets of cylindrical gears. A motor housing is fixedly connected to the outer wall of the hollow ring, and a servo motor is installed inside the motor housing. The output end of the servo motor is connected to the center of a set of cylindrical gears.

[0013] Furthermore, a steering mechanism is provided on the base plate. The steering mechanism includes a rotating ring that is movably fitted against the inner wall of the housing. A second pick-and-place groove is provided on the side wall of the rotating ring. An internal gear ring is fixedly connected to the bottom end of the rotating ring. The internal gear ring is embedded in the inside of the base plate and rotatably connected to the base plate. A stepper motor is embedded in the inside of the base plate. A drive gear is driven to the output end of the stepper motor. The drive gear meshes with the internal gear ring. An arc-shaped toothed plate is installed on the inner wall of the rotating ring.

[0014] A method for using a stem cell culture box with detection function, the method comprising: Culture flasks containing stem cell samples were placed into several sets of sample carriers for culturing. When it is necessary to test one of the stem cell samples, the second electric turntable is turned on, which drives the sample carrier assembly to move upward at a uniform and stable speed; When the sample-carrying assembly moves upward to the top of the spiral conveyor plate, the second electric turntable is turned off; The testing agency observed the stem cell samples within the sample-carrying assembly. After the test is completed, the sample carrier component moves downward after detaching from the spiral conveyor plate due to its own gravity. This squeezes the telescopic damping airbag, which slowly deflates while supporting the sample carrier component, allowing it to slowly descend to the base plate and complete the reset of the sample carrier component.

[0015] The beneficial effects of this invention are: 1. The spiral conveyor plate is driven to rotate by a second electric turntable. Combined with the limiting shaft and the limiting hole of the incomplete gear, a double guide is formed to position the movement trajectory of the sample carrier, effectively avoiding liquid surface fluctuations caused by tilting of the culture bottle during the ascent. With the air pressure regulation function controlled by the micro-flow one-way valve of the telescopic damping airbag, the sample carrier component can be stably and uniformly raised and lowered. Combined with the circular scanning of the electron microscope, multiple batches of samples can be tested without manual intervention. This effectively avoids the acceleration impact caused by traditional sample transfer methods, ensures the stability of the stem cell suspension, and effectively avoids the impact of mechanical vibration on stem cell growth, thereby effectively improving the accuracy and repeatability of stem cell detection data.

[0016] 2. The first electric turntable drives the movable frame to rotate, moving the electron microscope directly above the stem cell sample to be tested. As the electron microscope rotates, the light shield rotates synchronously. The electric turntable drives the light shield and the electron microscope in a synchronous manner, exposing only the transmission groove above the culture flask to be tested, effectively avoiding optical interference from adjacent samples. The nanofiber cloth covering the light shield automatically cleans the observation window glass during rotation, ensuring long-term observation clarity and improving the reliability of the test data.

[0017] 3. The rotating ring drives the arc-shaped toothed plate to rotate synchronously, so that the arc-shaped toothed plate rotates to the incomplete gear on the sample carrier assembly and meshes with it. The rotation of the arc-shaped toothed plate drives the incomplete gear to rotate synchronously, so that the sample carrier assembly rotates and extends to the outside of the first and second pick-up and drop-off slots. This facilitates the pick-up and drop-off of culture bottles, effectively improving the convenience of picking up and dropping culture bottles while avoiding contamination or interference to other samples.

[0018] 4. By placing the culture flask containing the stem cell sample into the sample carrier, the buffer plate effectively limits and protects it, further improving the stability of the culture flask during movement. The transparent magnetic cap makes it easy to open and close, facilitating the placement and removal of the culture flask. At the same time, the stem cell sample can be directly observed through the transparent magnetic cap, making the detection process more convenient and faster. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 A schematic diagram of the main structure according to an embodiment of the present invention is shown; Figure 2 A perspective view of the internal structure of the housing according to an embodiment of the present invention is shown; Figure 3 A schematic diagram of the conveying mechanism and sample carrier assembly according to an embodiment of the present invention is shown; Figure 4 A schematic diagram of the sample carrier assembly structure according to an embodiment of the present invention is shown; Figure 5 A cross-sectional view of the internal structure of the sample container according to an embodiment of the present invention is shown; Figure 6 An embodiment of the present invention is shown. Figure 5 Enlarged view of point A in the middle; Figure 7 A schematic diagram of the structure of the sample carrier assembly when it is moved to the observation position according to an embodiment of the present invention is shown; Figure 8 A schematic diagram of the internal structure of the box according to an embodiment of the present invention is shown; Figure 9 A schematic diagram of the steering mechanism structure according to an embodiment of the present invention is shown; Figure 10 A top view schematic diagram of the position of the sample-carrying component during sample loading and unloading according to an embodiment of the present invention is shown; Figure 11 A schematic diagram of the main structure for taking and placing samples according to an embodiment of the present invention is shown.

[0021] In the diagram: 100, box body; 110, first loading / unloading slot; 120, annular baffle plate; 130, rack; 140, hollow ring; 150, cylindrical gear; 160, motor box; 170, servo motor; 180, annular rack; 200, base plate; 300, cover plate; 310, annular observation window; 320, annular groove; 400, detection mechanism; 410, first electric turntable; 420, light shield; 421, fluoroscopic slot; 430, movable frame; 440, electron microscope; 450, support shaft; 460, roller; 500, conveying mechanism; 510, second electric turntable; 520, limiting shaft; 530, rotating shaft; 540, spiral conveyor plate; 550, arc-shaped abutment block; 560, telescopic damping airbag; 600, sample carrying assembly; 6 10. Incomplete gear; 620. Limiting hole; 630. Sample container; 631. First receiving groove; 632. Mounting groove; 633. Second receiving groove; 634. Sliding groove; 640. Buffer pad; 650. Sample loading groove; 660. Culture flask; 670. Transparent magnetic cap; 680. Abutment mechanism; 681. Abutment block; 682. Sliding shaft; 683. First spring; 684. Snap-fit ​​groove; 690. Snap-fit ​​part; 691. Cam; 692. Micro motor; 693. Wedge-shaped snap-fit ​​block; 694. Linkage shaft; 695. Extension plate; 696. Second spring; 700. Steering mechanism; 710. Rotating ring; 720. Second pick-and-place groove; 730. Internal gear ring; 740. Drive gear; 750. Stepper motor; 760. Arc-shaped gear plate. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] This invention provides a stem cell culture box with detection function, including a box body 100, a bottom plate 200, and a cover plate 300; for example, Figure 1 As shown.

[0024] The box body 100 is fixedly connected to the bottom plate 200, and the box body 100 is fixedly connected to the cover plate 300. The side wall of the box body 100 is provided with a plurality of first pick-up and put-out slots 110, and the plurality of first pick-up and put-out slots 110 are arranged in a circular array with the central axis of the box body 100 as the center. The box body 100 is provided with a constant temperature and humidity control component, which includes a controller, a temperature regulating device and a humidity regulating device. The cover plate 300 has an annular observation window 310, and a transparent glass is embedded in the annular observation window 310. The cover plate 300 is provided with a detection mechanism 400, which includes a first electric turntable 410. A light shield 420 is installed at the bottom center of the first electric turntable 410. The light shield 420 is movably fitted with the bottom end of the transparent glass installed on the annular observation window 310. The light shield 420 is made of a light-shielding material. The top of the light shield 420 is covered with a nanofiber cleaning cloth. A viewing groove 421 is provided on the light shield 420. A movable frame 430 is installed at the top center of the first electric turntable 410. An electron microscope 440 is installed on the movable frame 430 and is positioned directly above the fluoroscopy slot 421. A support shaft 450 is fixedly connected to the bottom of the other end of the movable frame 430. A roller 460 is provided at the bottom end of the support shaft 450. An annular groove 320 is provided on the cover plate 300, and the annular groove 320 is movably fitted with the roller 460. Specifically, stem cell samples can be placed into the housing 100 through the first loading slot 110. The first electric turntable 410 drives the movable frame 430 to rotate, moving the electron microscope 440 directly above the stem cell sample to be tested. As the electron microscope 440 rotates, the light shield 420 rotates synchronously, allowing the electron microscope 440 to examine the stem cell sample located directly below it through the annular observation window 310 and the perspective slot 421. The light shield 420 can block the view directly above other groups of stem cell samples to avoid interference with the detection. At the same time, when the light shield 420 rotates, the nanofiber cleaning cloth covering it can clean the transparent glass installed on the annular observation window 310, making the observation line clearer and thus improving the accuracy of the detection.

[0025] For example, such as Figures 2-3 As shown.

[0026] The housing 100 is equipped with a conveying mechanism 500, which includes a second electric turntable 510 and several sets of limiting shafts 520. The second electric turntable 510 is rotatably connected to the top center of the bottom plate 200. A rotating shaft 530 is fixedly connected to the center of the second electric turntable 510. The top of the rotating shaft 530 is rotatably connected to the bottom of the cover plate 300. A spiral conveying plate 540 is provided on the rotating shaft 530. An arc-shaped abutment block 550 is provided at the top of the rotating shaft 530. The arc-shaped abutment block 550 and the top of the spiral conveying plate 540 are in contact with each other. Several sets of limiting shafts 520 are fixedly connected between the cover plate 300 and the base plate 200. The several sets of limiting shafts 520 are arranged in a circular array with the central axis of the rotating shaft 530 as the center. Sample carriers 600 are slidably connected to each of the several sets of limiting shafts 520. Telescopic damping airbags 560 are sleeved on each of the several sets of limiting shafts 520. Each of the several sets of telescopic damping airbags 560 is arranged between a corresponding set of sample carriers 600 and the base plate 200. Each telescopic damping airbag 560 is provided with an air inlet pipe and an air outlet pipe. Each air inlet pipe and air outlet pipe is provided with a one-way valve with adjustable flow rate. Specifically, the second electric turntable 510 drives the rotating shaft 530 and the spiral conveyor plate 540 to rotate, causing the sample carrier 600 to slowly move upward along a corresponding set of limiting shafts 520 under the drive of the spiral conveyor plate 540. As the sample carrier 600 moves upward, the telescopic damping airbag 560 is stretched, allowing air to enter the telescopic damping airbag 560 through the air inlet pipe. When the sample carrier 600 rises to the top of the spiral conveyor plate 540 and detaches from it, the sample carrier 600 compresses the telescopic damping airbag 560 due to its own gravity. By adjusting the flow rate of the one-way valve of the air outlet pipe of the telescopic damping airbag 560 to a minimum, the air inside the telescopic damping airbag 560 is slowly discharged during the compression process, thereby supporting the sample carrier 600 and causing the sample carrier 600 to descend slowly. This effectively avoids the cell sample inside the sample carrier 600 from being jolted during rapid descent or movement, which could affect the normal growth of stem cells and the accuracy of the detection data.

[0027] For example, such as Figures 4-7 As shown.

[0028] The sample carrier assembly 600 includes an incomplete gear 610, the bottom end of which is fixedly connected to the top end of a telescopic damping airbag 560. A limiting hole 620 is formed at the center of the incomplete gear 610, and the limiting hole 620 is movably fitted with a corresponding set of limiting shafts 520. A sample carrier box 630 is fixedly connected to the incomplete gear 610. A buffer pad 640 is provided inside the sample carrier box 630. A sample carrier groove 650 is formed on the buffer pad 640. A culture bottle 660 is placed in the sample carrier groove 650. The culture bottle 660 contains stem cell samples. The culture bottle 660 is made of transparent material. A transparent magnetic cap 670 is provided on the sample carrier box 630. Specifically, by making the limiting hole 620 and the limiting shaft 520 fit together, the incomplete gear 610 keeps the sample container 630 stable during movement, preventing it from shifting and affecting normal observation. By placing the culture bottle 660 containing the stem cell sample into the sample tray 650, the buffer plate 640 effectively limits and protects it, further improving the stability of the culture bottle 660 during movement. The transparent magnetic cover 670 makes it easy to open and close, facilitating the placement and removal of the culture bottle 660, while also allowing direct observation of the stem cell sample through the transparent magnetic cover 670, making the detection process more convenient and faster.

[0029] The sample container 630 is provided with an abutment mechanism 680, which includes an abutment block 681. The abutment block 681 movably abuts against the upper surface of the rotating spiral conveyor plate 540. A sliding shaft 682 is fixedly connected to one side of the abutment block 681. The sample container 630 is provided with a first receiving groove 631, which is movably fitted with the abutment block 681. A first spring 683 is provided between the inner wall of the first receiving groove 631 and the abutment block 681. The first spring 683 is sleeved on the sliding shaft 682. The sample container 630 is provided with a sliding groove 634, which is movably fitted with the sliding shaft 682. Specifically, through the tension of the first spring 683, one end of the contact block 681 extends to the outside of the sample container 630. When the spiral conveyor plate 540 rotates, the bottom end of the spiral conveyor plate 540 rotates against the surface of the second electric turntable 510. When it rotates to the contact block 681 on the sample container 600, it can drive the contact block 681 to move upward along the surface of the spiral conveyor plate 540, thereby driving the culture flask 660 in the sample container 600 to move upward steadily and uniformly. When the sample container 600 moves upward to the top of the spiral conveyor plate 540, the culture flask 660 in the sample container 600 approaches the bottom end of the annular observation window 310 on the cover plate 300. At this time, the first electric turntable 410 can drive the electron microscope 440 to rotate directly above the culture flask 660 to detect the growth status of stem cells in the culture flask 660.

[0030] The sliding shaft 682 has a snap-fit ​​groove 684 on its side wall. The sample container 630 has an installation groove 632 and a second receiving groove 633. The sample container 630 has a snap-fit ​​part 690, which includes a cam 691 and a wedge-shaped snap-fit ​​block 693. The cam 691 is rotatably connected to the inner wall of the installation groove 632. A micro motor 692 is also installed in the installation groove 632. The output end of the micro motor 692 is connected to the center of the cam 691. The wedge-shaped snap-fit ​​block 694... 3. The wedge-shaped locking block 693 is movably engaged with the locking groove 684 and is movably fitted with the second receiving groove 633. The bottom end of the wedge-shaped locking block 693 is fixedly connected to the linkage shaft 694. A second spring 696 is provided between the wedge-shaped locking block 693 and the inner wall of the second receiving groove 633. The second spring 696 is sleeved on the linkage shaft 694. The bottom end of the linkage shaft 694 extends into the mounting groove 632 and is fixedly connected to the extension plate 695. The other end of the extension plate 695 is movably in contact with the cam 691. Specifically, after the testing of the culture flask 660 is completed, the second electric turntable 510 drives the rotating shaft 530 to continue rotating, so that the contact block 681 contacts the arc-shaped contact block 550. The arc-shaped contact block 550 squeezes the contact block 681, so that the contact block 681 enters the first receiving groove 631. At the same time, the sliding shaft 682 moves into the sliding groove 634. When the locking groove 684 moves to the wedge-shaped locking block 693, the wedge-shaped locking block 693 pops out and locks into the locking groove 684 through the tension of the second spring 696, so that the contact block 681 is separated from the spiral conveyor plate 540, so that the sample carrier assembly 600 slowly moves downward to the position of being in contact with the bottom plate 200 under the support of the telescopic damping airbag 560. Furthermore, when it is necessary to re-examine the stem cells in the culture flask 660, the micro motor 692 drives the cam 691 to rotate, causing the protrusion on the cam 691 to rotate directly above the extension plate 695 and press it. This causes the extension plate 695 to drive the linkage shaft 694 and the wedge-shaped locking block 693 to move downward synchronously, thereby causing the wedge-shaped locking block 693 to disengage from the locking groove 684. This causes the contact block 681 to pop out, allowing the sample carrier assembly 600 to move upward with the rotation of the spiral conveyor plate 540.

[0031] For example, such as Figures 8-11 As shown.

[0032] An annular baffle plate 120 is slidably connected to the bottom of the inner wall of the housing 100. An elastic sealing layer is embedded in the side wall of the annular baffle plate 120. Several sets of racks 130 are fixedly connected to the top of the annular baffle plate 120. A hollow ring 140 is fixedly connected to the outer wall of the housing 100. Several sets of columnar gears 150 are rotatably connected inside the hollow ring 140. One end of each set of columnar gears 150 passes through the hollow ring 140 and extends into the interior of the housing 100. Each set of columnar gears 150 is meshed with a corresponding set of racks 130. An annular rack 180 is rotatably connected to the bottom of the inner wall of the hollow ring 140. The annular rack 180 is meshed with several sets of columnar gears 150. A motor housing 160 is fixedly connected to the outer wall of the hollow ring 140. A servo motor 170 is installed inside the motor housing 160. The output end of the servo motor 170 is connected to the center of a set of columnar gears 150. Specifically, the servo motor 170 drives a set of cylindrical gears 150 to rotate, which in turn drives the annular rack 180 to drive the other sets of cylindrical gears 150 to rotate synchronously. This causes several sets of racks 130 to move the annular baffle 120 downwards, covering several sets of first pick-and-place slots 110. When it is necessary to pick up or place the culture bottle 660, the servo motor 170 rotates in the opposite direction, which drives the annular baffle 120 upwards into the box 100, thus preventing it from blocking the first pick-and-place slots 110.

[0033] A steering mechanism 700 is provided on the base plate 200. The steering mechanism 700 includes a rotating ring 710. The rotating ring 710 is movably fitted with the inner wall of the box 100. A second pick-and-place groove 720 is provided on the side wall of the rotating ring 710. The second pick-and-place groove 720 is the same size as several sets of first pick-and-place grooves 110. The bottom end of the rotating ring 710 is fixedly connected to an internal gear ring 730, which is embedded in the base plate 200 and rotatably connected to it. A stepper motor 750 is embedded in the base plate 200, and a drive gear 740 is driven to the output end of the stepper motor 750. The drive gear 740 meshes with the internal gear ring 730. An arc-shaped toothed plate 760 is installed on the inner wall of the rotating ring 710, and the arc-shaped toothed plate 760 intermittently meshes with several sets of incomplete gears 610. Specifically, the stepper motor 750 drives the drive gear 740 to rotate, and the internal gear ring 730 drives the rotating ring 710 to rotate synchronously, so that the second pick-and-place slot 720 can rotate to one side of the sample carrier assembly 600 to be picked and placed, so that the second pick-and-place slot 720 coincides with a set of first pick-and-place slots 110 corresponding to the sample carrier assembly 600. The culture bottle 660 on the sample carrier assembly 600 can be picked and placed through the first pick-and-place slot 110 and the second pick-and-place slot 720. Furthermore, while the rotating ring 710 rotates, the arc-shaped toothed plate 760 rotates synchronously, causing the arc-shaped toothed plate 760 to rotate to the incomplete gear 610 on the sample carrier assembly 600 and mesh with it. The rotation of the arc-shaped toothed plate 760 drives the incomplete gear 610 to rotate synchronously, causing the sample carrier assembly 600 to rotate and extend to the outside of the first pick-up and drop-off slot 110 and the second pick-up and drop-off slot 720, thereby facilitating the pick-up and drop-off of the culture bottle 660. After the pick-up and drop-off of the culture bottle 660 is completed, the stepper motor 750 drives the rotating ring 710 to rotate in the opposite direction, so that the sample carrier assembly 600 is reset and the annular barrier plate 120 moves downward to block several sets of the first pick-up and drop-off slots 110 to avoid interference from the external environment in the culture environment inside the box 100.

[0034] The working principle of the stem cell culture box with detection function proposed in this invention is as follows: The servo motor 170 drives a set of cylindrical gears 150 to rotate, which in turn drives the annular rack 180 to drive the other sets of cylindrical gears 150 to rotate synchronously. This causes several sets of racks 130 to move the annular baffle 120 downwards, covering several sets of first pick-and-place slots 110. When it is necessary to pick up or place the culture bottle 660, the servo motor 170 rotates in the opposite direction, which can drive the annular baffle 120 upwards into the box 100, so as to avoid it from blocking the first pick-and-place slots 110.

[0035] The stepper motor 750 drives the drive gear 740 to rotate, and the internal gear ring 730 drives the rotating ring 710 to rotate synchronously, so that the second pick-and-place slot 720 can rotate to one side of the sample carrier assembly 600 to be picked and placed, so that the second pick-and-place slot 720 coincides with a set of first pick-and-place slots 110 corresponding to the sample carrier assembly 600. The culture bottle 660 on the sample carrier assembly 600 can be picked and placed through the first pick-and-place slot 110 and the second pick-and-place slot 720. While the rotating ring 710 rotates, the arc-shaped toothed plate 760 rotates synchronously, causing the arc-shaped toothed plate 760 to rotate to the incomplete gear 610 on the sample carrier assembly 600 and mesh with it. The rotation of the arc-shaped toothed plate 760 drives the incomplete gear 610 to rotate synchronously, causing the sample carrier assembly 600 to rotate and extend to the outside of the first pick-and-place slot 110 and the second pick-and-place slot 720, thereby facilitating the pick-and-place of the culture bottle 660. After the pick-and-place operation of the culture bottle 660 is completed, the stepper motor 750 drives the rotating ring 710 to rotate in the opposite direction, so that the sample carrier assembly 600 is reset and the annular barrier plate 120 moves downward to block several sets of the first pick-and-place slots 110 to avoid interference from the external environment in the culture environment inside the box 100.

[0036] By placing the culture flask 660 containing the stem cell sample into the sample carrier 650, the buffer plate 640 effectively limits and protects it, further improving the stability of the culture flask 660 during movement. The transparent magnetic cover 670 is designed to facilitate the opening and closing of the culture flask 660, making it easy to pick up and put down. At the same time, the stem cell sample can be directly observed through the transparent magnetic cover 670, making the detection process more convenient and faster.

[0037] The tension of the first spring 683 causes one end of the contact block 681 to extend to the outside of the sample container 630. When the spiral conveyor plate 540 rotates, the bottom end of the spiral conveyor plate 540 rotates against the surface of the second electric turntable 510. When it rotates to the contact block 681 on the sample container 600, it can drive the contact block 681 to move upward along the surface of the spiral conveyor plate 540, thereby driving the culture bottle 660 in the sample container 600 to move upward steadily and uniformly. While the sample container 600 moves upward, the telescopic damping airbag 560 is stretched, allowing air to enter the telescopic damping airbag 560 through the air inlet pipe. When the sample container 600 moves upward to the top of the spiral conveyor plate 540, the culture bottle 660 in the sample container 600 approaches the bottom end of the annular observation window 310 on the cover plate 300.

[0038] The first electric turntable 410 drives the movable frame 430 to rotate, moving the electron microscope 440 directly above the stem cell sample to be tested. As the electron microscope 440 rotates, the light shield 420 rotates synchronously, allowing the electron microscope 440 to examine the stem cell sample located directly below it through the annular observation window 310 and the perspective groove 421. The light shield 420 can block the view directly above other groups of stem cell samples to avoid interference with the detection. At the same time, when the light shield 420 rotates, the nanofiber cleaning cloth covering it can clean the transparent glass installed on the annular observation window 310, making the observation line clearer and thus improving the accuracy of the detection.

[0039] After the testing of the culture flasks 660 is completed, the second electric turntable 510 drives the rotating shaft 530 to continue rotating, causing the contact block 681 to contact the arc-shaped contact block 550. The arc-shaped contact block 550 squeezes the contact block 681, causing the contact block 681 to enter the first receiving groove 631. At the same time, the sliding shaft 682 moves into the sliding groove 634. When the locking groove 684 moves to the wedge-shaped locking block 693, the tension of the second spring 696 causes the wedge-shaped locking block 693 to pop out and lock into the locking groove 684, thereby causing the contact block 681 to disengage from the spiral conveyor plate 540.

[0040] The sample carrier 600 compresses the telescopic damping airbag 560 due to its own gravity. By adjusting the flow rate of the one-way valve of the air outlet of the telescopic damping airbag 560 to a minimum, the air inside the telescopic damping airbag 560 is slowly discharged during the compression process. This allows the telescopic damping airbag 560 to support the sample carrier 600, enabling the sample carrier 600 to slowly descend and move to a position where it is in contact with the base plate 200. This effectively prevents the cell samples inside the sample carrier 600 from being jolted during rapid descent or movement, which could affect the normal growth of stem cells and the accuracy of the detection data.

[0041] When the stem cells in the culture flask 660 need to be tested again, the micro motor 692 drives the cam 691 to rotate, so that the protrusion on the cam 691 rotates to be directly above the extension plate 695 and squeezes it. This causes the extension plate 695 to drive the linkage shaft 694 and the wedge-shaped locking block 693 to move downward synchronously, thereby causing the wedge-shaped locking block 693 to disengage from the locking groove 684. This causes the contact block 681 to pop out, allowing the sample carrier assembly 600 to move upward with the rotation of the spiral conveyor plate 540.

[0042] Based on the aforementioned stem cell culture box with detection function, this embodiment of the invention also proposes a method of using the culture box. For example, the method of use includes: The servo motor is turned on to rotate in reverse, causing the annular baffle plate to move upwards into the interior of the housing. Turn on the stepper motor to rotate the second pick-and-place slot on the rotating ring to the sample carrier component that needs to be picked up or placed, so that the second pick-and-place slot coincides with the first pick-and-place slot. As the rotating ring rotates, the arc-shaped toothed plate drives the incomplete gear on the sample-carrying assembly to rotate, causing the sample box to rotate to the outside of the box. Open the transparent magnetic cover on the sample carrier box, place the culture flask containing the stem cell sample into the sample carrier slot, and then close the transparent magnetic cover. The stepper motor drives the rotating ring to rotate in the opposite direction, thus resetting the sample container; After repeating the above steps and placing several groups of culture flasks into the corresponding sample carrier box, turn on the servo motor to rotate, causing the annular baffle to move downwards and block the first and second pick-up slots. When it is necessary to test one of the stem cell samples, the micro motor in the sample carrier assembly is turned on, which drives the cam to rotate and disengage it from the extension plate, causing the contact block to pop out. Turn on the second electric turntable to make the spiral conveyor plate rotate and contact the bottom end of the contact block, thereby driving the sample carrier assembly to move upward at a uniform and stable speed. When the sample-carrying assembly moves upward to the top of the spiral conveyor plate, the second electric turntable is turned off; Turn on the first electric turntable, and the movable frame moves the electron microscope to directly above the sample-carrying assembly. At the same time, the light shield rotates synchronously, causing the fluoroscopy slot to rotate synchronously to directly above the sample-carrying assembly. The stem cell samples within the sample loading assembly were observed using an electron microscope. After the testing operation is completed, the second electric turntable is turned on to drive the spiral conveyor plate to continue rotating, so that the contact block contacts the arc-shaped contact block. The contact block retracts into the first receiving groove and is locked by the locking part, thereby separating the contact block from the spiral conveyor plate. Due to its own gravity, the sample carrier component moves downward after detaching from the spiral conveyor plate, which compresses the telescopic damping airbag. The damping airbag slowly deflates while supporting the sample carrier component, causing it to slowly descend to the bottom plate, thus completing the reset of the sample carrier component. By sequentially activating the micro-motors within several sample-carrying components, several groups of stem cell samples can be tested sequentially, and each sample can be automatically reset after testing.

[0043] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A stem cell culture box with detection function, comprising a box body, a bottom plate, and a cover plate, characterized in that: The cover plate is provided with an annular observation window, and the cover plate is provided with a detection mechanism for observing stem cells; The box is equipped with a conveying mechanism, which includes a second electric turntable and several sets of limiting shafts. The second electric turntable is rotatably connected to the center of the top of the bottom plate. A rotating shaft is fixedly connected to the center of the second electric turntable. A spiral conveying plate for conveying stem cell samples is provided on the rotating shaft. An arc-shaped abutment block is provided at the top of the rotating shaft, and the arc-shaped abutment block is in contact with the top of the spiral conveying plate. Each of the aforementioned limiting shafts is slidably connected to a sample loading component for loading stem cell samples. Several sets of limiting shafts are fitted with telescopic damping airbags for buffering and limiting the speed of the sample carrier assembly, and several sets of telescopic damping airbags are set between the corresponding set of sample carrier assembly and the base plate. The telescopic damping airbag is equipped with an air inlet pipe and an air outlet pipe, and both the air inlet pipe and the air outlet pipe are equipped with a one-way valve with adjustable flow rate.

2. The stem cell culture box with detection function according to claim 1, characterized in that: The box body is fixedly connected to the bottom plate, and the box body is fixedly connected to the cover plate. The side wall of the box body is provided with several sets of first pick-up and put-out slots, and the several sets of first pick-up and put-out slots are arranged in a circular array with the central axis of the box body as the center. The box body is provided with a constant temperature and humidity control component, which includes a controller, a temperature adjustment device and a humidity adjustment device.

3. The stem cell culture box with detection function according to claim 1, characterized in that: The testing mechanism includes a first electric turntable, a light-shielding plate installed at the center of the bottom end of the first electric turntable, a nanofiber cleaning cloth covering the top of the light-shielding plate, a viewing groove formed on the light-shielding plate, a movable frame installed at the center of the top end of the first electric turntable, an electron microscope mounted on the movable frame, a support shaft fixedly connected to the bottom of the other end of the movable frame, a roller provided at the bottom end of the support shaft, and an annular groove formed on the cover plate, the annular groove being in movable contact with the roller.

4. The stem cell culture box with detection function according to claim 1, characterized in that: The sample carrier assembly includes an incomplete gear, the bottom end of which is fixedly connected to the top end of the telescopic damping airbag. A limiting hole is provided at the center of the incomplete gear, and the limiting hole is movably fitted with a corresponding set of limiting shafts.

5. The stem cell culture box with detection function according to claim 4, characterized in that... A sample carrier box is fixedly connected to the incomplete gear. A buffer pad is provided inside the sample carrier box. A sample carrier slot is opened on the buffer pad. A culture bottle is placed in the sample carrier slot. The culture bottle contains stem cell samples. The culture bottle is made of transparent material. A transparent magnetic cap is provided on the sample carrier box.

6. The stem cell culture box with detection function according to claim 5, characterized in that: The sample container is equipped with an abutting mechanism, which includes an abutting block. The abutting block movably abuts against the upper surface of the rotating spiral conveyor plate. A sliding shaft is fixedly connected to one side of the abutting block. A first receiving groove is provided on the sample container, which movably fits against the abutting block. A first spring is provided between the inner wall of the first receiving groove and the abutting block. The first spring is sleeved on the sliding shaft. A sliding groove is provided on the sample container, which movably fits against the sliding shaft.

7. The stem cell culture box with detection function according to claim 6, characterized in that: The sliding shaft has a snap-fit ​​groove on its side wall. The sample container has an installation groove and a second receiving groove. The sample container has a snap-fit ​​part, which includes a cam and a wedge-shaped snap-fit ​​block. The cam is rotatably connected to the inner wall of the installation groove. A micro motor is also installed in the installation groove. The output end of the micro motor is connected to the center of the cam. The wedge-shaped snap-fit ​​block is movably snapped into the snap-fit ​​groove and movably fitted into the second receiving groove. A linkage shaft is fixedly connected to the bottom end of the wedge-shaped snap-fit ​​block. A second spring is provided between the wedge-shaped snap-fit ​​block and the inner wall of the second receiving groove. The second spring is sleeved on the linkage shaft. The bottom end of the linkage shaft extends into the installation groove and is fixedly connected to an extension plate. The other end of the extension plate movably abuts against the cam.

8. The stem cell culture box with detection function according to claim 1, characterized in that: An annular baffle plate is slidably connected to the bottom of the inner side of the housing. Several sets of racks are fixedly connected to the top of the annular baffle plate. A hollow ring is fixedly connected to the outer wall of the housing. Several sets of cylindrical gears are rotatably connected inside the hollow ring. One end of each set of cylindrical gears passes through the hollow ring and extends into the interior of the housing. Each set of cylindrical gears meshes with a corresponding set of racks. An annular rack is rotatably connected to the bottom of the inner wall of the hollow ring. The annular rack meshes with several sets of cylindrical gears. A motor housing is fixedly connected to the outer wall of the hollow ring. A servo motor is installed inside the motor housing. The output end of the servo motor is connected to the center of a set of cylindrical gears.

9. The stem cell culture box with detection function according to claim 1, characterized in that: A steering mechanism is provided on the base plate. The steering mechanism includes a rotating ring that is movably fitted against the inner wall of the housing. A second pick-and-place groove is provided on the side wall of the rotating ring. An internal gear ring is fixedly connected to the bottom end of the rotating ring. The internal gear ring is embedded in the base plate and rotatably connected to the base plate. A stepper motor is embedded in the base plate. A drive gear is driven to the output end of the stepper motor. The drive gear meshes with the internal gear ring. An arc-shaped toothed plate is installed on the inner wall of the rotating ring.

10. A method of using a stem cell culture box with detection function as described in any one of claims 1-9, characterized in that: The method of use includes: Culture flasks containing stem cell samples were placed into several sets of sample carriers for culturing. When it is necessary to test one of the stem cell samples, the second electric turntable is turned on, which drives the sample carrier assembly to move upward at a uniform and stable speed; When the sample-carrying assembly moves upward to the top of the spiral conveyor plate, the second electric turntable is turned off; The testing agency observed the stem cell samples within the sample-carrying assembly. After the test is completed, the sample carrier component moves downward after detaching from the spiral conveyor plate due to its own gravity. This squeezes the telescopic damping airbag, which slowly deflates while supporting the sample carrier component, allowing it to slowly descend to the base plate and complete the reset of the sample carrier component.

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