A ginseng extract rich in ginsenoside Rb and a method for extracting the same

Abstract

The application discloses a ginseng extract rich in ginsenoside Rb and an extraction method thereof, and relates to the technical field of traditional Chinese medicine extraction and separation. The method takes the dried roots of 5-year-old and younger artificially planted ginseng as raw materials, and obtains ginseng coarse powder through cleaning, slicing, drying and crushing; after adding water to the ginseng coarse powder and adjusting the pH, a composite enzyme preparation is added for enzymatic pretreatment, and after inactivating the enzyme activity, a pretreated liquid is obtained; ethanol and water are added to the pretreated liquid, the ethanol concentration is adjusted, and twice hot reflux extraction is carried out and filtration is performed; after the extract is combined and concentrated under reduced pressure, water is added, stirred and allowed to stand for impurity removal, and the supernatant is taken for adsorption resin purification; the ethanol eluate is collected and concentrated under reduced pressure, malt dextrin is added and uniformly mixed, and then spray drying is performed, so as to obtain the ginseng extract rich in ginsenoside Rb. Compared with the prior art, the ginseng extract prepared by the method is beneficial to improving the extract yield, the ginsenoside content and the antioxidant activity.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine extraction and separation technology, and in particular to a ginseng extract rich in ginsenoside Rb and its extraction method. Background Technology

[0002] Ginseng, belonging to the genus Panax (Panax Ginseng CAMeyer) of the Araliaceae family, is one of my country's traditional medicinal and edible resources. Its roots contain a relatively rich variety of active substances, including saponins, sugars, amino acids, polypeptides, phenols, and trace volatile components, giving it high nutritional, functional, and industrial development value. With the deepening research into the active ingredients of natural plants, cultivated ginseng has been widely used in the food, health food, cosmetics, and related health product fields, and is gradually becoming one of the important raw materials for developing naturally derived effective active ingredients in the broader health industry.

[0003] Among the various active ingredients in ginseng, ginsenosides are considered one of its important functional material bases. Based on different structural types and glycosyl substitution methods, ginsenosides can be divided into several types and have good development potential in various application scenarios, thus attracting continuous attention in this field. In addition to saponins, ginseng also contains certain amounts of phenols, sugars, amino acids, and peptides, which, together with saponins, constitute the active material basis of ginseng extracts. Phenolic components contribute to antioxidant activity, while sugars, amino acids, and peptides can influence the color, taste, stability, and product compatibility of the extract to a certain extent.

[0004] CN110898091A discloses a ginseng extract and its preparation method. The technical solution involves slicing and washing ginseng roots, then refluxing with 60-80% ethanol. The resulting filtrate is concentrated under reduced pressure, purified by macroporous resin, washed with water to remove impurities, desorbed with 50-70% ethanol, treated with pesticide residues and resin, concentrated again, and spray-dried to obtain the ginseng extract. CN103142669B discloses an enzymatic extraction method for ginsenosides. The technical solution involves using an aqueous solution containing an added enzyme as the enzyme extractant to extract sliced ​​or pulverized ginseng. The enzyme is cellulase, pectinase, or a mixture thereof. Ethanol is then added to the enzymatic hydrolysate to prepare a 30-60% (v / v) ethanol extract, which is then heated and refluxed for extraction. The residue is then refluxed again, and the extracts are combined. While existing technologies have improved ginseng extraction processes in their respective directions, they still cannot simultaneously solve the problems of insufficient cell wall disruption, inadequate release of intracellular active ingredients, low enrichment efficiency of target saponins, low extract yield, and insufficient release and retention of bound phenolic active ingredients. In practical use, this can easily lead to problems such as unstable extraction efficiency, large fluctuations in the content of active ingredients in the product, and insufficient antioxidant activity. Summary of the Invention

[0005] In view of the above-mentioned defects of existing extraction and purification technologies, the technical problem to be solved by the present invention is to provide a ginseng extract rich in ginsenoside Rb and its extraction method, so as to solve the problems of insufficient cell wall disruption of ginseng tissue, insufficient release of intracellular effective components, low enrichment efficiency of target saponins, low yield of extract, and insufficient release of active components such as bound phenols in the existing extraction process, thereby obtaining a ginseng extract with high content of target saponins, high yield and good antioxidant activity.

[0006] To achieve the above objectives, the present invention provides a ginseng extract rich in ginsenoside Rb and its extraction method.

[0007] A method for extracting ginseng extract rich in ginsenoside Rb includes the following steps: using dried roots of artificially cultivated ginseng as raw material, the ginseng is washed, sliced, dried, and pulverized to obtain crude ginseng powder; the crude ginseng powder is subjected to two reflux extractions using an ethanol-water solution; the two extracts are combined and concentrated under reduced pressure, then impurities are removed by adding water, stirring, and allowing to settle, resulting in a sample solution to be purified; stepwise elution purification is performed using an adsorption resin, and the ethanol eluent rich in ginsenoside Rb is collected; the ethanol eluent is concentrated under reduced pressure, maltodextrin is added and mixed, and then spray-dried to obtain ginsenoside Rb-rich ginseng extract.

[0008] Preferably, the artificially cultivated ginseng is ginseng that has been artificially cultivated for 5 years or less.

[0009] The thickness of the ginseng slices is 2-4 mm; the drying temperature is 50-60℃, the drying time is 4-8 h, and the moisture content of the raw material after drying is 6-10%; after pulverization, it is passed through an 18-24 mesh sieve.

[0010] Preferably, the volume fraction of ethanol in the aqueous ethanol solution used in the two hot reflux extractions is 55-65%; the material-to-liquid ratio for the first extraction is 1 kg:(7-9) L, the extraction temperature is 75-85℃, the stirring speed is 120-180 rpm, and the extraction time is 1.5-2.5 h; the material-to-liquid ratio for the second extraction is 1 kg:(5-7) L, the extraction temperature is 75-85℃, the stirring speed is 120-180 rpm, and the extraction time is 1-2 h.

[0011] Preferably, the combined extracts are concentrated under reduced pressure at 55-60℃ and -0.06 to -0.09 MPa, while ethanol is recovered simultaneously, until the relative density of the concentrate at 60℃ is 1.10-1.15; water is added to the concentrated extract, and the mixture is stirred at 80-120 rpm for 15-25 min, followed by standing for 8-12 h.

[0012] Preferably, the adsorption resin is AB-8 type macroporous adsorption resin, and the resin bed volume is 80-120L; the sample loading flow rate is 0.8-1.2 BV / h; after the sample loading is completed, it is first eluted with 1.5-2.5 BV of water, then eluted with 0.5-1.5 BV of ethanol aqueous solution with a volume fraction of 15-25%, and the aforementioned eluent is discarded; subsequently, it is eluted with 2.5-3.5 BV of ethanol aqueous solution with a volume fraction of 70-80%, and the alcohol eluent is collected.

[0013] Preferably, the alcohol eluent is concentrated under reduced pressure at 55-60℃ and -0.06 to -0.09 MPa until the solid content is 12-18 wt%; maltodextrin is added to the concentrate, the amount of maltodextrin added being 3-8 wt% of the solid content in the concentrate; after stirring at 100-140 rpm for 20-40 min, spray drying is performed; the inlet air temperature of the spray drying is 170-190℃, the outlet air temperature is 80-90℃, and the atomization pressure is 0.5-0.7 MPa.

[0014] Conventional physical pretreatment and solvent reflux extraction methods have limited effect on disrupting the cell structure of ginseng tissues, resulting in insufficient release of intracellular saponins and other active ingredients. Furthermore, there is a lack of targeted release methods for bound phenols and active ingredients tightly bound to the cell wall structure.

[0015] Preferably, a method for extracting ginseng extract rich in ginsenoside Rb includes the following steps: cleaning and slicing dried roots of artificially cultivated ginseng into thin slices, mixing them with water, and pretreating them with a pulsed electric field; drying and pulverizing the pretreated raw material to obtain pretreated ginseng powder; adding water to the pretreated ginseng powder and adjusting the pH, then adding a compound enzyme preparation for enzymatic hydrolysis pretreatment, and obtaining a pretreated solution after enzyme inactivation; adding ethanol and water to the pretreated solution, adjusting the ethanol concentration, and performing two reflux extractions; combining the extracts, concentrating under reduced pressure, adding water, stirring, and allowing to stand to remove impurities, and purifying the supernatant with an adsorption resin; collecting the ethanol eluent, concentrating under reduced pressure, adding maltodextrin, mixing well, and spray drying to obtain ginseng extract rich in ginsenoside Rb.

[0016] Preferably, in the pulsed electric field pretreatment stage, the mass-to-volume ratio of ginseng slices to water is 1 kg: 1.5-2.5 L; the electric field strength of the pulsed electric field treatment is 1.5-2 kV / cm; the pulse width is 20-40 μs; the pulse frequency is 250-350 Hz; the number of pulses is 50-70; the treatment time is 1-3 min; and the treatment temperature is controlled below 35℃.

[0017] Preferably, in the enzymatic hydrolysis pretreatment stage, the material-to-liquid ratio is 1 kg: 3-5 L; the system pH is 4.5-5.5; the total amount of compound enzyme preparation added is 0.35-0.55 wt% of the ginseng crude powder; the enzymatic hydrolysis temperature is 40-50℃, the enzymatic hydrolysis time is 50-70 min; the stirring speed is 100-140 rpm; the enzyme inactivation temperature is 80-90℃, and the enzyme inactivation time is 8-12 min.

[0018] The enzyme preparation is at least one of xylanase, pectic acid lyase and ferulic acid esterase; preferably, the compound enzyme preparation is a mixture of xylanase, pectic acid lyase and ferulic acid esterase in a mass ratio of (1-5):(1-3):1.

[0019] A further preferred method for extracting ginseng extract rich in ginsenoside Rb includes the following steps: cleaning and slicing dried roots of artificially cultivated ginseng into thin slices, mixing them with water, and pretreating them with a pulsed electric field; drying and pulverizing the pretreated raw material to obtain pretreated ginseng powder; adding water to the pretreated ginseng powder, adjusting the pH, adding an enzymatic hydrolysis aid, mixing evenly to obtain a premixed solution, then adding a compound enzyme preparation for enzymatic hydrolysis pretreatment, and obtaining a pretreated solution after enzyme inactivation; adding ethanol and water to the pretreated solution, adjusting the ethanol concentration, and performing two reflux extractions; combining the extracts, concentrating under reduced pressure, adding water, stirring, and allowing to stand to remove impurities, and purifying the supernatant with an adsorption resin; collecting the ethanol eluent, concentrating under reduced pressure, adding maltodextrin, mixing evenly, and spray drying to obtain ginseng extract rich in ginsenoside Rb.

[0020] Preferably, the amount of the enzymatic hydrolysis aid added is 0.015-0.080 wt% of the ginseng crude powder; the enzymatic hydrolysis aid is at least one of calcium gluconate and casein phosphopeptide; preferably, the enzymatic hydrolysis aid is a mixture of calcium gluconate and casein phosphopeptide in a mass ratio of (1-3):(1-5).

[0021] Calcium gluconate provides a mild and readily available source of calcium ions in a weakly acidic enzymatic hydrolysis system at pH 5. The pectic acid lyase in the compound enzyme preparation is sensitive to calcium ions; an appropriate amount of calcium ions helps maintain its cleavage effect on the pectin layer and intercellular adhesion structures, thereby promoting the loosening of the cell wall and intercellular matrix structure of ginseng tissue. Casein phosphopeptides contain phosphorylated structural units, which can form a relatively stable dispersed binding state with calcium ions, preventing excessively high local concentrations of calcium ions or rapid precipitation. This ensures that the calcium ions released from calcium gluconate maintain good dispersibility and continuous availability in the enzymatic hydrolysis system. The combined effect of these two enzymes enhances the sustained action of pectic acid lyase, which, along with xylanase's degradation of the hemicellulose skeleton and ferulic acid esterase's cleavage of polysaccharide-phenolic ester bonds, allows for a more complete release of ginsenoside Rb compounds that were previously bound by the cell wall, polysaccharide network, and intercellular layer. Furthermore, due to the increased enzymatic hydrolysis efficiency, subsequent ethanol reflux extraction does not require further increases in temperature or time to achieve sufficient component migration, reducing the loss of some phenols and other antioxidant active ingredients during prolonged heat treatment.

[0022] The present invention also provides a ginseng extract rich in ginsenoside Rb prepared by the above method.

[0023] This invention also provides an application of ginseng extract rich in ginsenoside Rb.

[0024] The ginseng extract rich in ginsenoside Rb prepared by the present invention can be used as a functional raw material in the fields of food, health food, cosmetics and related health products.

[0025] The ginseng extract rich in ginsenoside Rb obtained by this invention can be formulated into various preparations according to different application requirements.

[0026] In food or health food applications, the ginseng extract can be made into powders, granules, instant powders, solid beverages, oral liquids, syrups, pastes, tablets, compressed candies, hard capsules, soft capsules, gummies, and beverage preparations.

[0027] In cosmetic applications, the ginseng extract can be formulated into solutions, emulsions, creams, serums, gels, masks, sprays, and other topical preparations.

[0028] Preferably, when the ginseng extract is made into the above-mentioned preparation, it can be compounded with food-grade acceptable excipients, commonly used excipients in health foods, or cosmetic-grade acceptable matrices according to the product type.

[0029] Furthermore, the ginseng extract can be used alone as an active ingredient, or it can be used in combination with other plant extracts, sugars, vitamins, minerals, amino acids, colloidal matrices, or conventional excipients.

[0030] When ginseng slices are subjected to a pulsed electric field, the permeability of the cell membrane and cell wall system can be enhanced at a lower temperature, weakening the obstruction of intracellular components by the dense tissue structure, thus creating more favorable mass transfer conditions for subsequent enzymatic hydrolysis and solvent extraction. Subsequently, a complex enzyme system consisting of xylanase, pectic acid lyase, and ferulic acid esterase is introduced, making it easier to release saponins, phenols, and other difficult-to-release active ingredients that were previously bound by the cell wall. Then, reflux extraction with an ethanol-water solution is used to more fully transfer the released or easily migrating saponins and some polyphenols into the extract. Afterwards, some insoluble impurities are removed by adding water and allowing the mixture to stand, followed by selective purification using AB-8 macroporous adsorption resin, further enriching the target saponin components while reducing impurity interference. Finally, stable powder is obtained by vacuum concentration, addition of maltodextrin, and spray drying.

[0031] Xylanase primarily degrades the xylan backbone in hemicellulose, weakening the cell wall support network; pectic acid lyase mainly disrupts the pectin layer and intercellular adhesion structures, reducing tissue density; ferulic acid esterase helps to cleave some ester bonds between cell wall polysaccharides and phenolic acids, making previously bound phenols and other difficult-to-release active ingredients more easily dissociated. The synergistic effect of these three enzymes enhances the solvent's penetration into the cell interior during ethanol extraction and reduces the retention of active ingredients in the cell wall backbone, thereby promoting the simultaneous release of ginsenoside Rb compounds and total phenolic components.

[0032] The beneficial effects of the present invention are as follows: 1. Compared with the prior art, the present invention introduces pulsed electric field pretreatment before conventional solvent extraction, which can improve the permeability of ginseng tissue cell membrane and cell wall system under lower temperature conditions, weaken the obstruction of the dense structure of the tissue to the migration of intracellular active ingredients, and create more favorable mass transfer conditions for subsequent enzymatic pretreatment and ethanol reflux extraction, thereby improving the release efficiency of ginsenosides and other active ingredients.

[0033] 2. Compared with the prior art, the present invention, based on pulsed electric field pretreatment, further introduces a complex enzyme preparation composed of xylanase, pectic acid lyase and ferulic acid esterase for enzymatic pretreatment. The three work synergistically to further weaken the cell wall support network and promote the dissociation and migration of saponins, phenols and other difficult-to-release active ingredients that were originally bound by the cell wall. Therefore, it is beneficial to improve the yield of extract and the content of active ingredients. Detailed Implementation

[0034] The endpoints and any values ​​of the ranges disclosed in this invention are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of the various ranges, the endpoint values ​​of the various ranges and individual point values, and individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this invention.

[0035] AB-8 type macroporous adsorption resin was purchased from Cangzhou Baoen Adsorption Materials Technology Co., Ltd.

[0036] Xylanase was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., product number X195724.

[0037] Pectic acid lyase was purchased from Shanghai Jinpan Biotechnology Co., Ltd., product number E-PLYCJ.

[0038] The ferulic acid esterase was purchased from Shanghai Jinpan Biotechnology Co., Ltd., with the product number ACZ05862.

[0039] Other raw materials not mentioned are all common raw materials. The above content is only for the purpose of illustrating the present invention and should not be construed as a strict limitation of the present invention. Those skilled in the art can directly purchase the same / similar raw materials from the market or prepare them themselves.

[0040] The technical solutions of the present invention will be clearly and completely described below with reference to specific embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Example 1

[0041] A method for extracting ginseng extract rich in ginsenoside Rb includes the following steps: Step 1: Weigh 100 kg of dried roots of artificially cultivated ginseng (dried roots of ginseng with a growth period of 5 years or less), remove impurities, wash them with water, drain the surface moisture, and cut them into ginseng slices with a thickness of 3 mm; Place the ginseng slices in a hot air drying oven and dry them at 55°C for 6 hours to reduce the moisture content of the raw material to 8%; Then, pulverize the dried ginseng slices and pass them through a 20-mesh sieve to obtain coarse ginseng powder.

[0042] Step 2: Add the crude ginseng powder obtained in Step 1 to the extraction tank, and add 800L of 60% (v / v) ethanol aqueous solution; start stirring at 150 rpm and reflux at 80℃ for 2 hours; after extraction, filter in a filter and collect the first extract; add the filtered residue back to the extraction tank, and add 600L of 60% (v / v) ethanol aqueous solution; reflux at 150 rpm and 80℃ for 1.5 hours; after extraction, filter and collect the second extract; combine the first and second extracts to obtain the combined extract; transfer the obtained combined extract to a vacuum concentrator, concentrate under reduced pressure at 58℃ and -0.08MPa while simultaneously recovering ethanol, concentrate until the relative density of the concentrate at 60℃ is 1.12, and obtain the concentrated extract.

[0043] Step 3: Add 200L of water to the concentrated extract obtained in Step 2, stir at 100rpm for 20min to mix evenly, and then let stand for 10h. After standing, remove the bottom precipitate, take the supernatant to obtain the sample solution to be purified. Use AB-8 macroporous adsorption resin as the purification medium, pack the resin into a resin column, and the resin bed volume is 100L. First, pretreat the resin column with 95% ethanol and water in sequence until the eluent has no obvious alcohol odor. Then, load the obtained sample solution to be purified into the resin column at a flow rate of 1BV / h. After loading, elute with 2BV of water, then elute with 1BV of 20% ethanol aqueous solution, and discard the above eluent. Then, elute with 3BV of 75% ethanol aqueous solution, collect this part of the eluent, and obtain the alcohol eluent rich in ginsenoside Rb.

[0044] Step 4: Transfer the alcohol eluent obtained in Step 3 into a vacuum concentrator, concentrate and recover ethanol under reduced pressure at 58℃ and -0.08MPa until the solid content is 15wt%, and obtain a concentrated solution; add maltodextrin to the obtained concentrated solution slurry, the amount of maltodextrin added is 5wt% of the solid content in the concentrated solution; stir at 120rpm for 30min to obtain a spray drying feed liquid; send the feed liquid into a spray drying tower for spray drying, control the inlet air temperature at 180℃, the outlet air temperature at 85℃, and the atomization pressure at 0.60MPa; collect the powder after spray drying; pulverize the obtained powder and pass it through an 80-mesh sieve to obtain a ginseng extract rich in ginsenoside Rb. Test Example 1

[0045] The product obtained in Example 1 is a light yellow fine powder with the inherent taste and odor of this product, without any burnt or other off-odors.

[0046] The method for determining the Rb1 content was as follows: the content of ginsenoside Rb1 in the ginseng extract obtained in the example was determined by high performance liquid chromatography; the experimental results showed that the content of ginsenoside Rb1 was 40.53%.

[0047] Table 1. Physicochemical properties of the ginseng extract rich in ginsenoside Rb obtained in Example 1. Test Example 2

[0048] Cardiotonic effect test: Fifty SPF-grade male SD rats, weighing 200-220g, were selected and, after 7 days of acclimatization, were randomly divided into four groups: normal group, model group, low-dose group (Example 1), medium-dose group (Example 1), and high-dose group (Example 1), with 10 rats in each group. Except for the normal group, chronic heart failure models were established in the other groups by intraperitoneal injection of doxorubicin hydrochloride, with a cumulative dose of 15mg / kg. After model establishment, drug administration was initiated after echocardiographic confirmation of a significant decrease in left ventricular ejection fraction in the model animals.

[0049] The normal group and the model group were administered the same volume of purified water by gavage daily; the low-dose group, medium-dose group and high-dose group of Example 1 were administered the ginseng extract obtained in Example 1 by gavage at 50 mg / kg, 100 mg / kg and 200 mg / kg respectively, once a day for 28 consecutive days.

[0050] Twenty-four hours after the last administration, echocardiography was performed on rats in each group to record the left ventricular ejection fraction (LVEF). Subsequently, abdominal aortic blood was collected, serum was separated, and the serum N-terminal pro-brain natriuretic peptide (NT-proBNP) content was measured by ELISA. TGF-β1 protein expression level was detected by Western blot on left ventricular free wall tissue, and normalization analysis was performed with the relative expression level of the normal group set as 1.00.

[0051] Table 2. Effects of ginseng extract obtained in Example 1 on cardiac function indicators in rats with heart failure.

[0052]

[0053] As shown in Table 2, the model group rats exhibited a significant decrease in LVEF, a significant increase in serum NT-proBNP levels, and a significant upregulation of TGF-β1 protein expression in myocardial tissue, indicating the successful establishment of the chronic heart failure model. Compared with the model group, the ginseng extract obtained in Example 1 increased LVEF, decreased NT-proBNP levels, and downregulated TGF-β1 protein expression, with the improvement trend becoming more pronounced with increasing dosage. These results demonstrate that the ginseng extract obtained in Example 1 possesses good cardiotonic and cardioprotective effects. Test Example 3

[0054] Anti-shock effect test: Fifty SPF-grade male SD rats, weighing 200-220g, were randomly divided into a normal group, a model group, a low-dose group (Example 1), a medium-dose group (Example 1), and a high-dose group (Example 1), with 10 rats in each group. The normal group and the model group were administered an equal volume of purified water by gavage daily. The low-dose group, the medium-dose group, and the high-dose group (Example 1) were administered the ginseng extract obtained in Example 1 by gavage at doses of 50mg / kg, 100mg / kg, and 200mg / kg, respectively, once daily for 7 consecutive days.

[0055] One hour after the last administration on day 7, endotoxin shock models were established in all groups except the control group by intraperitoneal injection of lipopolysaccharide (LPS) 10 mg / kg; the control group received an equal volume of physiological saline intraperitoneally. Six hours after modeling, mean arterial pressure (MAP) was measured via femoral artery cannulation; blood samples were collected simultaneously, serum was separated, and NO content was measured using ELISA; the survival rate of each group within 24 hours after modeling was recorded and calculated.

[0056] Table 3. Effects of ginseng extract obtained in Example 1 on key indicators in rats with endotoxin shock.

[0057]

[0058] As shown in Table 3, the mean arterial pressure of the rats in the model group was significantly decreased, serum NO levels were significantly increased, and the 24-hour survival rate was significantly reduced, indicating that the endotoxin shock model was successfully established. Compared with the model group, the ginseng extract obtained in Example 1 could increase mean arterial pressure, decrease NO levels, and improve the 24-hour survival rate, and the improvement trend was more obvious with increasing dosage. The above results indicate that the ginseng extract obtained in Example 1 has a good anti-shock effect. Example 2

[0059] A method for extracting ginseng extract rich in ginsenoside Rb includes the following steps: Step 1: Weigh 100 kg of dried roots of artificially cultivated ginseng (dried roots of ginseng with a growth period of 5 years), remove impurities, wash with water, drain the surface water, and cut into ginseng slices with a thickness of 3 mm; add the obtained ginseng slices to a pretreatment tank, add water, so that the mass-volume ratio of ginseng slices to water is 1 kg: 2 L, stir at 80 rpm for 5 min until uniform, and then send to a pulse electric field treatment device for treatment; The pulsed electric field treatment conditions are as follows: electric field strength 1.8 kV / cm, pulse width 30 µs, pulse frequency 300 Hz, number of pulses 60, treatment time 2 min, and treatment temperature controlled below 30 ℃. After the pulsed electric field treatment, the ginseng slices are taken out and the surface moisture is drained. The treated ginseng slices are placed in a hot air drying oven and dried at 55 ℃ for 6 h to reduce the moisture content of the raw material to 8%. Then the dried ginseng slices are crushed and passed through a 20-mesh sieve to obtain ginseng coarse powder pretreated by the pulsed electric field.

[0060] Step 2: Add the obtained ginseng crude powder to an enzymatic hydrolysis tank, add water, and control the material-to-liquid ratio to 1 kg: 4 L; adjust the pH of the system to 5 using a citrate-sodium citrate buffer solution; then add a compound enzyme preparation, wherein the total amount of the compound enzyme preparation added is 0.45 wt% of the ginseng crude powder mass. The compound enzyme preparation is composed of xylanase, pectic acid lyase, and ferulic acid esterase in a mass ratio of 5:3:1. Enzymatic hydrolysis is carried out at 45°C and 120 rpm for 60 min, with the stirring speed controlled at 120 rpm; after the enzymatic hydrolysis is completed, the system is heated to 85°C and kept at this temperature for 10 min to inactivate the enzyme, and then cooled to room temperature to obtain a pretreated solution.

[0061] Step 3: Add ethanol and water to the pretreated solution obtained above, adjust the ethanol volume fraction of the system to 60%, and make the total material-to-liquid ratio 1kg:8L; start stirring at 150rpm and reflux extraction at 78℃ for 2h; after extraction, filter in a filter and collect the first extract; add the filtered residue back into the extraction tank, add 60% ethanol aqueous solution to make the material-to-liquid ratio 1kg:6L, and reflux extraction at 150rpm and 78℃ for 1.5h; after extraction, filter and collect the second extract; combine the first extract and the second extract to obtain the combined extract; transfer the obtained combined extract to a vacuum concentrator, concentrate under reduced pressure at 58℃ and -0.08MPa while simultaneously recovering ethanol, concentrate until the relative density of the concentrate at 60℃ is 1.12, to obtain the concentrated extract.

[0062] Step 4: Add 200L of water to the concentrated extract obtained in Step 3, stir at 100rpm for 20min to mix evenly, and then let stand for 10h. After standing, remove the bottom precipitate, take the supernatant to obtain the sample solution to be purified. Use AB-8 macroporous adsorption resin as the purification medium, pack the resin into a resin column, and the resin bed volume is 100L. First, pretreat the resin column with 95% ethanol and water in sequence until the eluent has no obvious alcohol odor. Then, load the obtained sample solution to be purified into the resin column at a flow rate of 1BV / h. After loading, elute with 2BV purified water, then elute with 1BV of 20% ethanol aqueous solution, and discard the above eluent. Then elute with 3BV of 75% ethanol aqueous solution, collect this part of the eluent, and obtain ginsenoside Rb-rich ginsenoside Rb-rich ginsenoside Rb eluent.

[0063] Step 5: Transfer the alcohol eluent obtained in Step 4 into a vacuum concentrator, concentrate and recover ethanol under reduced pressure at 58℃ and -0.08MPa until the solid content is 15wt%, and obtain a concentrated solution; add maltodextrin to the obtained concentrated solution, the amount of maltodextrin added being 5wt% of the solid content in the concentrated solution; stir at 120rpm for 30min to obtain a spray drying feed solution; send the feed solution into a spray drying tower for spray drying, controlling the inlet air temperature at 180℃, the outlet air temperature at 85℃, and the atomization pressure at 0.60MPa; collect the powder after spray drying; pulverize the obtained powder and pass it through an 80-mesh sieve to obtain a ginseng extract rich in ginsenoside Rb. Example 3

[0064] An extraction method for ginseng extract rich in ginsenoside Rb is basically the same as that in Example 2, except that the compound enzyme preparation is composed of xylanase and pectic acid lyase in a mass ratio of 5:3. Example 4

[0065] An extraction method for ginseng extract rich in ginsenoside Rb is basically the same as that in Example 2, except that the compound enzyme preparation is composed of pectinase and ferulic acid esterase in a mass ratio of 3:1. Example 5

[0066] An extraction method for ginseng extract rich in ginsenoside Rb is basically the same as that in Example 2, except that the compound enzyme preparation is composed of xylanase and ferulic acid esterase in a mass ratio of 5:1. Example 6

[0067] An extraction method for ginseng extract rich in ginsenoside Rb is basically the same as that in Example 2, except that a single enzyme preparation xylanase is used, wherein the total amount of xylanase added is 0.45 wt% of the crude ginseng powder. Example 7

[0068] An extraction method for ginseng extract rich in ginsenoside Rb is basically the same as that in Example 2, except that a single enzyme preparation, pectic acid lyase, is used, wherein the total amount of pectic acid lyase added is 0.45 wt% of the crude ginseng powder. Example 8

[0069] An extraction method for ginseng extract rich in ginsenoside Rb is basically the same as that in Example 2, except that a single enzyme preparation, ferulic acid esterase, is used, wherein the total amount of ferulic acid esterase added is 0.45 wt% of the crude ginseng powder. Example 9

[0070] An extraction method for ginseng extract rich in ginsenoside Rb is basically the same as that in Example 2, except that step 2 is different.

[0071] Step 2: Add the obtained ginseng crude powder to an enzymatic hydrolysis tank, add water, and control the material-to-liquid ratio to 1 kg: 4 L; adjust the pH of the system to 5 using a citrate-sodium citrate buffer solution; separately take an enzymatic hydrolysis aid, the amount of which is 0.035 wt% of the ginseng crude powder mass, the enzymatic hydrolysis aid being composed of calcium gluconate and casein phosphopeptide in a mass ratio of 3:4; take 0.02 L of water per 1 kg of ginseng crude powder as the preparation water for the enzymatic hydrolysis aid, and include the preparation water for the enzymatic hydrolysis aid in the total water volume added in Step 2 at a material-to-liquid ratio of 1 kg: 4 L; when the ginseng crude powder is 100 kg, take 2 L of water as the preparation water for the enzymatic hydrolysis aid. Add the enzymatic hydrolysis aid to the above 2 L of water, stir at 120 rpm for 5 min to dissolve the calcium gluconate and uniformly disperse the casein phosphopeptide, obtaining an aqueous solution of the enzymatic hydrolysis aid. The obtained aqueous solution of enzymatic hydrolysis aid was added to the enzymatic hydrolysis tank, with the stirring speed controlled at 120 rpm during the addition process. After the addition was completed, stirring was continued for 10 min. Subsequently, a compound enzyme preparation was added, wherein the total amount of the compound enzyme preparation added was 0.45 wt% of the ginseng crude powder. The compound enzyme preparation consisted of xylanase, pectic acid lyase, and ferulic acid esterase in a mass ratio of 5:3:1. Enzymatic hydrolysis was carried out at 45℃ and 120 rpm for 60 min. After the enzymatic hydrolysis was completed, the system was heated to 85℃ and kept at this temperature for 10 min to inactivate the enzyme. Then, it was cooled to room temperature to obtain the pretreated solution. Example 10

[0072] An extraction method for ginseng extract rich in ginsenoside Rb is basically the same as that in Example 9, except that the enzymatic hydrolysis aid is calcium gluconate. Example 11

[0073] An extraction method for ginseng extract rich in ginsenoside Rb is basically the same as that in Example 9, except that the enzymatic hydrolysis aid is casein phosphopeptide.

[0074] Comparative Example 1: An extraction method for ginseng extract rich in ginsenoside Rb, which is basically the same as that in Example 2, except that: in step 1, the ginseng slices are subjected to pulsed electric field treatment; in step 2, no compound enzyme preparation is added and no enzymatic pretreatment is performed.

[0075] After being treated with a pulsed electric field, the ginseng slices were directly dried and pulverized, then subjected to two reflux extractions with 60% ethanol according to step 3, and then purified, concentrated and spray-dried according to steps 4 and 5.

[0076] Comparative Example 2: An extraction method for ginseng extract rich in ginsenoside Rb, which is basically the same as that in Example 2, except that pulsed electric field treatment is not performed in step 1.

[0077] In step 2, the same compound enzyme preparation as in Example 2 was used for enzymatic pretreatment. The total amount of the compound enzyme preparation added was 0.45 wt% of the ginseng crude powder. The compound enzyme preparation consisted of xylanase, pectic acid lyase and ferulic acid esterase in a mass ratio of 5:3:1. Enzymatic hydrolysis was carried out at 45°C and 120 rpm for 50 min with stirring. After the enzymatic hydrolysis was completed, the temperature was raised to 85°C and kept at that temperature for 10 min to inactivate the enzyme. The remaining steps were the same as in Example 2.

[0078] Comparative Example 3: An extraction method for ginseng extract rich in ginsenoside Rb, which is basically the same as that in Example 2, except that in step 1, the ginseng slices are subjected to pulsed electric field treatment under the same conditions as in Example 2.

[0079] In step 2, instead of using the xylanase, pectic acid lyase, and ferulic acid esterase complex enzyme system of Example 2, a conventional complex enzyme preparation is used. The total amount of the conventional complex enzyme preparation added is 0.45 wt% of the ginseng crude powder. The conventional complex enzyme preparation is composed of cellulase, pectinase, and α-amylase mixed in a mass ratio of 3:2:1. Enzymatic hydrolysis is carried out at 45°C and 120 rpm for 60 min with stirring. After the enzymatic hydrolysis is completed, the temperature is raised to 85°C and kept at this temperature for 10 min to inactivate the enzyme. The remaining steps are the same as in Example 2. Test Example 4

[0080] Samples were prepared according to the methods of the examples and comparative examples. All groups used dried roots of artificially cultivated ginseng from the same batch, origin, and year as raw materials, and were prepared under the same resin purification, vacuum concentration, and spray drying conditions. Three batches were prepared independently for each group, and the test results are expressed as the average of the three parallel batches.

[0081] Test method for extract yield: Weigh the mass of the extract obtained after spray drying of each group of samples, and calculate the extract yield according to the following formula: Extract yield (%) = Mass of extract / Mass of dried ginseng root raw material × 100%.

[0082] The quality of ginseng dried root raw materials is calculated on an absolutely dry basis.

[0083] Table 4 Test results of extraction effect Test Example 5

[0084] Determination of Total Ginsenoside Content: It should be noted that the total ginsenoside content, expressed as ginsenoside Rb1 in this test example, refers to the Rb1 equivalent of total saponin components determined by the vanillin-perchloric acid colorimetric method, and not the absolute content of a single ginsenoside Rb1. The content of a single ginsenoside Rb1 can be determined separately by high-performance liquid chromatography. This test example uses this index to compare the effects of different extraction processes on the overall release and enrichment of saponin components.

[0085] The total ginsenoside content, expressed as ginsenoside Rb1 equivalent, in the samples of each example and comparative example was determined by the vanillin-perchloric acid colorimetric method.

[0086] Determination principle: After heating and color development in the vanillin-perchloric acid system, the saponin components in the sample produce characteristic absorption at visible light wavelengths. A standard curve is established using ginsenoside Rb1 as a reference standard. The total ginsenoside content in the sample, calculated as ginsenoside Rb1 equivalent, is obtained based on the absorbance of the test solution. The results are expressed as mass percentage.

[0087] Preparation of reference stock solution: Weigh 10 mg of ginsenoside Rb1 reference standard dried to constant weight, place it in a 50 mL volumetric flask, add an appropriate amount of methanol, sonicate to dissolve completely, and after cooling to room temperature, dilute to the mark with methanol, shake well to obtain a reference stock solution with a mass concentration of 0.20 mg / mL.

[0088] Preparation of a series of reference standard working solutions: Accurately pipette 0.5 mL, 1 mL, 2 mL, 3 mL, 4 mL and 5 mL of the reference standard stock solution into 10 mL volumetric flasks, dilute to the mark with methanol, and shake well to obtain a series of reference standard working solutions with mass concentrations of 0.01 mg / mL, 0.02 mg / mL, 0.04 mg / mL, 0.06 mg / mL, 0.08 mg / mL and 0.10 mg / mL, respectively.

[0089] Preparation of the test solution: Weigh 50 mg of the samples obtained from each example and comparative example, place them in a 50 mL volumetric flask, add 35 mL of methanol to fully wet and disperse the sample, and ultrasonically extract in an ultrasonic cleaner for 30 min; the ultrasonic conditions are: frequency 40 kHz, power 250 W, and water bath temperature controlled below 25 °C. After ultrasonication, remove the sample, cool it to room temperature, and then add methanol to the mark and shake well; centrifuge the resulting solution at 10000 r / min for 10 min, and take the supernatant to obtain the original test solution.

[0090] Accurately pipette 5 mL of the original test solution into a 50 mL volumetric flask, dilute to the mark with methanol, and shake well to obtain the test solution. The dilution factor of the test solution relative to the original test solution is 10.

[0091] Colorimetric reaction and absorbance determination: Accurately pipette 0.5 mL of each series of reference standard working solution or test sample test solution into a stoppered test tube, add 0.5 mL of 5% vanillin glacial acetic acid solution, then add 4.0 mL of perchloric acid, mix thoroughly, and heat in a 60℃ constant temperature water bath for 15 min; remove and immediately place in an ice water bath to cool to room temperature, then add 5.0 mL of glacial acetic acid and shake well.

[0092] Prepare a blank control solution by replacing the reference working solution or the test sample assay solution with methanol and following the same procedure. Zero the instrument with the blank control solution and measure the absorbance of each series of reference working solutions and test sample assay solutions at a wavelength of 560 nm.

[0093] Establishment of the standard curve: A standard curve was established with the mass concentration of ginsenoside Rb1 reference standard as the abscissa and the corresponding absorbance as the ordinate, yielding a linear regression equation. The total ginsenoside mass concentration in the test solution, expressed as ginsenoside Rb1 equivalent, was calculated based on this standard curve.

[0094] The total ginsenoside content (%), calculated as ginsenoside Rb1, is calculated as [(C×V×n) / m]×100%.

[0095] Where: C is the total ginsenoside concentration in the test solution calculated based on ginsenoside Rb1 according to the standard curve, in mg / mL; V is the final volume of the test solution, in mL; n is the dilution factor of the test solution before determination; and m is the sample weight of the test sample, in mg.

[0096] Three test solutions were prepared in parallel for each group of samples and measured separately. The arithmetic mean of the three parallel measurements was taken as the final result. The relative standard deviation of the three parallel measurements was preferably no greater than 5%.

[0097] DPPH free radical scavenging rate determination: Weigh each group of samples and prepare a sample solution of 1 mg / mL with 50% ethanol. Take 1 mL of sample solution, add 3 mL of 0.1 mmol / L DPPH ethanol solution, mix well, and react in the dark for 30 min to obtain the sample reaction solution; take 1 mL of 50% ethanol, add 3 mL of 0.1 mmol / L DPPH ethanol solution, mix well, and react in the dark for 30 min to obtain the blank control solution; take 1 mL of sample solution, add 3 mL of 50% ethanol, mix well, and let stand for 30 min to obtain the sample background solution. Measure the absorbance of the sample reaction solution, blank control solution, and sample background solution at a wavelength of 517 nm, and record them as A1, A0, and A2, respectively.

[0098] The DPPH radical scavenging rate is calculated using the following formula: DPPH radical scavenging rate (%) = [1 - (A1 - A2) / A0] × 100%.

[0099] Where: A0 is the absorbance of the blank control solution; A1 is the absorbance of the sample reaction solution; A2 is the absorbance of the sample background solution.

[0100] Each sample was measured in parallel three times, and the arithmetic mean of the three parallel measurements was taken as the final result.

[0101] Table 5. Results of test on active ingredient content and antioxidant properties

[0102] As shown in Tables 4-5, Example 1 only used conventional ethanol reflux extraction, while Example 2 added pulsed electric field pretreatment and enzymatic hydrolysis pretreatment with a specific compound enzyme preparation before extraction. In Example 1, the ginseng tissue did not undergo directional cell wall disruption and selective enzymatic hydrolysis before entering the ethanol system. The cell membrane, cell wall, and intercellular layer still strongly hindered the migration of intracellular components, resulting in insufficient release of saponins and bound phenols. Ultimately, this resulted in significantly lower extract yield, total ginsenoside content, and antioxidant activity. In Example 2, the permeability of the cell membrane and cell wall system was first increased by a pulsed electric field. Then, xylanase, pectic acid lyase, and ferulic acid esterase were used to specifically and synergistically dismantle the hemicellulose skeleton, pectin layer, and polysaccharide-phenolic acid interester bonds. Therefore, the extract yield increased from 1.92% to 2.28%, the total ginsenoside content increased from 57.8% to 66.1%, and the DPPH free radical scavenging rate increased from 43.2% to 68.9%.

[0103] Comparative Example 1 retained the pulsed electric field treatment but omitted the enzymatic hydrolysis step using the compound enzyme preparation. The main function of the pulsed electric field is to increase tissue permeability and weaken physical barriers, improving the difficulty for solvents to enter the tissue. However, it cannot directly sever the hemicellulose xylan backbone, disrupt the pectin layer adhesion structure, or effectively break some ester bonds between cell wall polysaccharides and phenolic acids. Therefore, although Comparative Example 1 showed slight improvement over Example 1, it was still significantly lower than Example 2, with an extract yield of only 1.98%, a total ginsenoside content of only 58.4%, and a DPPH free radical scavenging rate of only 46.3%. Comparative Example 2 used the same compound enzyme preparation as Example 2 but did not undergo pulsed electric field treatment, and the hydrolysis time was shortened from 60 min to 50 min. Due to the lack of pulsed electric field pretreatment, the permeability of the ginseng tissue's cell membrane and cell wall was not sufficiently improved before hydrolysis, limiting the contact between enzyme molecules and subsequent extraction solvents with the internal structures. Simultaneously, the shortened hydrolysis time further reduced the degree of disassembly of key cell wall structures by the compound enzyme system. Therefore, although Comparative Example 2 was better than Example 1 and Comparative Example 1, it was still lower than Example 2, with an extract yield of 2.19%, a total ginsenoside content of 64.2%, and a DPPH free radical scavenging rate of 64.7%.

[0104] Comparative Example 3 also underwent pulsed electric field treatment, but a conventional compound system of cellulase, pectinase, and α-amylase was used instead in the enzymatic hydrolysis stage. This conventional system can act on some cell wall components or storage polysaccharides, but its target sites are different from those of the xylanase, pectic acid lyase, and ferulic acid esterase used in this application. In particular, the conventional system is not directly effective in weakening the hemicellulose xylan support network, and its ability to cleave the ester bonds between cell wall polysaccharides and phenolic acids is significantly insufficient. Furthermore, α-amylase mainly targets starch substrates and has limited targeting of the dense structure of ginseng cell walls. Therefore, although Comparative Example 3 is still superior to Example 1, its extract yield is only 2.08%, its total ginsenoside content is only 59.5%, and its DPPH free radical scavenging rate is only 54.2%, all significantly lower than that of Example 2.

[0105] Examples 3 to 5 employed different dual-enzyme combinations, while Examples 6 to 8 each used a single enzyme preparation. Although these schemes all retained pulsed electric field treatment, the reduced dimensionality of action after enzyme system fragmentation prevented them from reaching the level of Example 2. Specifically, Example 3 used xylanase and pectic acid lyase, which could act on both the hemicellulose backbone and the pectin layer simultaneously. Therefore, its extract yield and total ginsenoside content were relatively high in the dual-enzyme group, at 2.16% and 62.9%, respectively. However, due to the lack of ferulic acid esterase, the dissociation of bound phenols and polysaccharide-phenolic acid linkages was insufficient, resulting in a DPPH free radical scavenging rate of only 60.8%, lower than that of Examples 4 and 5, which contained ferulic acid esterase.

[0106] Example 9, based on the enzymatic hydrolysis system of the compound enzyme preparation in Example 2, further adds an enzymatic hydrolysis aid composed of calcium gluconate and casein phosphopeptide in a mass ratio of 3:4. Calcium gluconate provides mild and readily available calcium ions in a weakly acidic system at pH 5, which helps maintain the cleavage effect of pectin acid lyase on the pectin layer and intercellular adhesion structures, promoting the loosening of ginseng cell walls and intercellular matrix. Casein phosphopeptide contains phosphorylated structural units, which can form a stable dispersion with calcium ions, avoiding local accumulation or precipitation of calcium ions and improving the sustained availability of calcium ions. The synergistic effect of the two enhances the sustained action of pectin acid lyase and, together with xylanase and ferulic acid esterase, further disrupts the cell wall network and polysaccharide-phenolic acid linkage structure, allowing for a more complete release of ginsenoside Rb components and antioxidant active ingredients, while reducing the loss of active ingredients during subsequent thermal extraction.

[0107] The preferred embodiments of the present invention have been described in detail above. It should be understood that those skilled in the art can make numerous modifications and variations based on the concept of the present invention without creative effort. Therefore, all technical solutions that can be obtained by those skilled in the art based on the concept of the present invention through logical analysis, reasoning, or limited experimentation on the basis of existing technology should be within the scope of protection defined by the claims.

Claims

1. A method for extracting a ginseng extract enriched with ginsenoside Rb from ginseng, characterized by, Includes the following steps: Using artificially cultivated dried ginseng roots as raw material, ginseng crude powder was obtained through washing, slicing, drying, and pulverizing. Water was added to the ginseng crude powder and the pH was adjusted. Then, a compound enzyme preparation was added for enzymatic hydrolysis pretreatment. After inactivation of the enzyme, a pretreated solution was obtained. Ethanol and water were added to the pretreated solution, and the ethanol concentration was adjusted. The solution was then subjected to two hot reflux extractions and filtered. The extracts were combined, concentrated under reduced pressure, and then water was added, stirred, and allowed to stand to remove impurities. The supernatant was then purified using an adsorption resin. The ethanol eluent was collected and concentrated under reduced pressure. Maltodextrin was added, mixed well, and then spray-dried to obtain a ginseng extract rich in ginsenoside Rb. The compound enzyme preparation is at least one of xylanase, pectic acid lyase and ferulic acid esterase.

2. The extraction method of ginseng extract rich in ginsenoside Rb as described in claim 1, characterized in that: Pulsed electric field treatment was performed before adding the compound enzyme preparation.

3. The extraction method of ginseng extract rich in ginsenoside Rb as described in claim 2, characterized in that: In the pulsed electric field treatment stage, the mass-to-volume ratio of ginseng slices to water is 1 kg: 1.5-2.5 L; the electric field strength of the pulsed electric field treatment is 1.5-2 kV / cm; the pulse width is 20-40 μs; the pulse frequency is 250-350 Hz; the number of pulses is 50-70; the treatment time is 1-3 min; and the treatment temperature is controlled below 35℃.

4. The extraction method of ginseng extract rich in ginsenoside Rb as described in claim 1, characterized in that: In the enzymatic hydrolysis pretreatment stage, the material-to-liquid ratio is 1 kg: 3-5 L; the system pH is 4.5-5.5; the enzymatic hydrolysis temperature is 40-50℃, the enzymatic hydrolysis time is 50-70 min; the stirring speed is 100-140 rpm; the enzyme inactivation temperature is 80-90℃, and the enzyme inactivation time is 8-12 min.

5. The extraction method of ginseng extract rich in ginsenoside Rb as described in claim 1, characterized in that: The ethanol-water solution used in the two reflux extractions has an ethanol volume fraction of 55-65%; the material-to-liquid ratio for the first extraction is 1 kg:(7-9) L, the extraction temperature is 75-85℃, the stirring speed is 120-180 rpm, and the extraction time is 1.5-2.5 h; the material-to-liquid ratio for the second extraction is 1 kg:(5-7) L, the extraction temperature is 75-85℃, the stirring speed is 120-180 rpm, and the extraction time is 1-2 h.

6. The extraction method of ginseng extract rich in ginsenoside Rb as described in claim 1, characterized in that: The extract was concentrated under reduced pressure at 55-60℃ and -0.06 to -0.09 MPa, with ethanol being recovered simultaneously, until the relative density of the concentrate at 60℃ was 1.10-1.

15. Water was added to the concentrated extract, and the mixture was stirred at 80-120 rpm for 15-25 min, followed by standing for 8-12 h.

7. The extraction method of ginseng extract rich in ginsenoside Rb as described in claim 1, characterized in that: The adsorption resin is AB-8 type macroporous adsorption resin; after the sample is loaded, it is first eluted with 1.5-2.5 BV of water, then eluted with 0.5-1.5 BV of ethanol aqueous solution with a volume fraction of 15-25%, and the eluent is discarded; then eluted with 2.5-3.5 BV of ethanol aqueous solution with a volume fraction of 70-80%, and the alcohol eluent is collected.

8. The extraction method of ginseng extract rich in ginsenoside Rb as described in claim 1, characterized in that: The alcohol eluent is concentrated under reduced pressure at 55-60℃ and -0.06 to -0.09 MPa until the solid content is 12-18 wt%. Maltodextrin is added to the concentrate at an amount of 3-8 wt% of the solid content. After stirring at 100-140 rpm for 20-40 min, the solution is spray-dried. The inlet air temperature of the spray dryer is 170-190℃, the outlet air temperature is 80-90℃, and the atomization pressure is 0.5-0.7 MPa.

9. A ginseng extract rich in ginsenoside Rb, characterized in that: Extracted by the extraction method described in any one of claims 1-8.

10. The application of the ginseng extract rich in ginsenoside Rb as described in claim 9 in food, health products, cosmetics or related health products.

Images