PLC-based intelligent optical microscope stage

Abstract

This invention provides a PLC-based intelligent optical microscope stage, belonging to the field of microscope technology. Through the cooperation of a mechanical support module, a two-dimensional motion module, a control and drive module, and an image acquisition adapter module, it achieves automated and accurate detection and full-area image acquisition of biological tissue samples. The mechanical support module adopts a double-layer support structure consisting of an ABS honeycomb mounting plate and a 3D-printed cylindrical support frame, combining lightweight and high rigidity, effectively isolating vibration and providing a stable bearing foundation for each functional module. The two-dimensional motion module forms an X-Y axis motion platform through two SGX ball screw single-track slides orthogonally fixed, and, in conjunction with a z-axis manual lifting stage, achieves three-dimensional adjustment of the slide stage. The control and drive module uses a PLC controller in conjunction with a two-axis stepper motor controller to achieve automated stepping control, enabling full-area scanning of the sample according to a preset path, significantly improving detection efficiency and imaging accuracy, and meeting the needs of observation and scientific research analysis.

Description

Technical Field

[0001] This invention belongs to the field of microscope technology, and more specifically, relates to a PLC-based intelligent optical microscope stage. Background Technology

[0002] In the field of optical microscope-assisted observation, the stage is the core component for supporting biological tissue samples and adjusting the observation position. It is widely used in biomedical testing, pathological diagnosis, and scientific research analysis. Traditional stages are mostly designed for manual adjustment, and the adjustment accuracy depends on the operator's experience and hand stability. They lack standardized positioning mechanisms and have obvious technical limitations.

[0003] The movement accuracy is difficult to control, the step distance consistency is poor, and repeated positioning is prone to positional deviation due to human error.

[0004] Without an automated drive structure, the field of view at high magnification is limited to a micrometer-level area. Full-area scanning of the sample requires manual and repeated movement of the stage and point-by-point recording, which is cumbersome and time-consuming.

[0005] Manual positioning errors can also cause seams and texture misalignment in image stitching, with errors far exceeding the imaging accuracy requirements.

[0006] The aforementioned problems prevent traditional stages from efficiently acquiring complete and clear panoramic images of samples. Pathological observation and scientific research analysis have extremely high requirements for the identification of morphological features such as cell arrangement and cell nucleus morphology. Existing images, due to large stitching errors and lack of details, cannot reflect the true pathological state of the sample and cannot meet the core needs of accurate observation and quantitative analysis, thus limiting the application depth of optical microscopes. Therefore, this invention provides a PLC-based intelligent optical microscope stage to solve the above problems. Summary of the Invention

[0007] To address the aforementioned technical problems, this invention provides a PLC-based intelligent optical microscope stage.

[0008] A PLC-based intelligent optical microscope stage includes a mechanical support module, a two-dimensional motion module, a control drive module, and an image acquisition adapter module.

[0009] Preferably, the mechanical support module is used to support the two-dimensional motion module, the control drive module, and the image acquisition adapter module. The mechanical support module includes an ABS honeycomb mounting plate and a cylindrical support frame. The ABS honeycomb mounting plate has a size of 370mm×270mm and there are 2 of them. The cylindrical support frame is a 3D printed connecting column made of PLA, there are 7 of them, and the height is 8cm. It is used to reinforce the connection between the upper ABS honeycomb mounting plate and the lower mounting surface.

[0010] Preferably, the two-dimensional motion module includes a first SGX ball screw single-track slide, a second SGX ball screw single-track slide, a four-hole cross connector, a Z-axis lifting platform, and a slide stage. Both the first and second SGX ball screw single-track slides are SGX series ball screw single-track slides with a stroke of 250mm and a repeatability of ±0.01mm. The first and second SGX ball screw single-track slides are orthogonally connected by the four-hole cross connector to form an XY-axis motion platform. The four-hole cross connector is a component of the SGX slides. The slide stage is made of aluminum alloy and has four M4 screw holes on its upper and lower surfaces. It is fixed above the XY-axis motion platform via a Z-axis lifting platform. The Z-axis lifting platform is a manual Z-axis lifting platform with a stroke of 20mm and an adjustment accuracy of 0.01mm. The slide stage is a 3D printed part made of PLA and is sized to fit standard slides. The inner side of the slide stage has a positioning structure adapted to the slide, which is a micro-sunken design with a depth of 0.2mm. The edge of the slide stage has a gripping opening for easy placement and removal of slides, and the gripping opening has a semi-rounded corner structure with a radius of 6mm.

[0011] Preferably, the control drive module can control the movement of the two-dimensional motion module. The control drive module includes a PLC controller, a first two-axis stepper motor controller, a second two-axis stepper motor controller, and a matching 110V power supply. The PLC controller is selected from the FBs series. The first and second two-axis stepper motor controllers are both SGX slide table matching controllers. The PLC controller is electrically connected to the first and second two-axis stepper motor controllers. The first and second two-axis stepper motor controllers are electrically connected to the stepper motors in the first and second SGX ball screw single-track slide tables, respectively. The matching 110V power supply is a 110V DC power supply with an output power of 500W, which powers the control drive module to realize the stepping control of the two-dimensional motion module. The control drive module also includes a PLC controller bracket. The PLC controller bracket is a 3D printed part made of PLA and has a snap-fit ​​structure adapted to the PLC controller. The PLC controller is fixed to the ABS honeycomb mounting plate by the bracket.

[0012] Preferably, the two-dimensional motion module can move the slide stage to perform microscopic observation in conjunction with the image acquisition adapter module. The image acquisition adapter module includes a microscope body, a microscope support, and an electronic eyepiece. The microscope support is made of cast iron, has a height of 337mm, and is fixed to an ABS honeycomb mounting plate. It supports the microscope body and aligns the objective lens with the slide stage. The microscope body is a 400x optical microscope, and the electronic eyepiece is a 10x electronic eyepiece used to acquire electronic images. The image acquisition adapter module also includes an electronic eyepiece protective sleeve. The electronic eyepiece protective sleeve is a 3D printed part with an inner diameter adapted to the electronic eyepiece. It is made of PLA and serves as a protective and anti-shake function.

[0013] Preferably, a mounting frame is fixedly installed on the side wall of the microscope body. Two sets of air blowers and fiber optic sensors are respectively provided on the inner side of the mounting frame. Each set of air blowers and fiber optic sensors is parallel to each other. The fiber optic sensors are used to detect whether there is a slide on the positioning structure of the slide stage. The distance between each set of air blowers and fiber optic sensors is the distance between the positioning structures on the slide stage.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] In this invention, a double-layer support structure is formed by an ABS honeycomb mounting plate and a cylindrical support frame. The cylindrical support frame consists of 3D-printed connecting columns evenly distributed on the bottom edge of the upper mounting plate. Combined with 3D-printed PLC controller brackets, protective covers for electronic eyepieces, and other compatible components, this structure combines lightweight design with high rigidity. It can effectively isolate vibration and ensure stable cooperation between the microscope body, electronic eyepiece, and stage. The structure is stable and highly adaptable.

[0016] In this invention, the XY axis motion path and stepping parameters are preset by the PLC controller, and the fiber optic sensor automatically detects whether a glass slide is placed in the positioning structure of the glass slide stage. The air blower cleans the surface of the glass slide by blowing air during the image acquisition interval, so there is no need for repeated manual adjustment and recording. The sample full-area scanning and image acquisition can be completed automatically according to the preset path. After the acquisition is completed, the XY axis motion platform automatically returns to the initial position, which greatly simplifies the operation process and improves the efficiency of panoramic image acquisition.

[0017] In this invention, a PLC controller, in conjunction with a first two-axis stepper motor controller and a second two-axis stepper motor controller, drives the stepper motors within the first SGX ball screw single-track slide and the second SGX ball screw single-track slide, respectively. The first SGX ball screw single-track slide and the second SGX ball screw single-track slide are orthogonally connected by a four-hole cross connector to form an XY-axis motion platform, thereby achieving automated and precise stepping movement of the glass slide in the XY-axis direction. The step distance is consistent, and the repeatability can reach ±0.01mm, effectively solving the problem of insufficient precision in traditional manual stages.

[0018] In this invention, images are collected by setting up a control drive module and a two-dimensional motion module. High-precision automated positioning reduces the overlap and omission of images in adjacent areas, providing high-quality raw data for subsequent panoramic image stitching. Combined with SIFT feature matching algorithm for duplicate area detection, dynamic erosion optimization module for regional non-uniform erosion processing, and Poisson fusion technology to eliminate seam artifacts, the stitching error is effectively reduced, ensuring natural transition of cell edges and no distortion of cell nucleus morphology, thus meeting the precise needs of pathological observation and scientific research analysis.

[0019] In this invention, a mounting frame is fixedly installed on the side wall of the microscope body. Two sets of air blowers and fiber optic sensors are respectively set on the inner side of the mounting frame. Each set of air blowers and fiber optic sensors is parallel to each other, and the spacing is adapted to the spacing between the positioning structures on the slide stage. This enables multi-station synchronous detection and cleaning. The fiber optic sensors can automatically detect whether a slide is placed in the positioning structure of the slide stage, ensuring accurate triggering of the subsequent image acquisition process. The air blowers can blow air to clean the surface of the slide, removing dust to ensure image clarity and improve detection efficiency and image quality. Attached Figure Description

[0020] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0021] Figure 2 This is a schematic diagram of the mechanical support module assembly structure of the present invention;

[0022] Figure 3 This is a schematic diagram of the two-dimensional motion module combination structure of the present invention;

[0023] Figure 4 This is a schematic diagram of the control drive module combination structure of the present invention;

[0024] Figure 5 This is a schematic diagram of the microscope body assembly structure of the present invention;

[0025] Figure 6 This is a schematic diagram of the mounting bracket assembly structure of the present invention.

[0026] In the diagram, the correspondence between the component names and the attached drawing numbers is as follows: 1. ABS honeycomb mounting plate; 2. Cylindrical support frame; 3. Slide stage; 4. Z-axis lifting stage; 5. First SGX ball screw single-track slide; 6. Four-hole cross connector; 7. Second SGX ball screw single-track slide; 8. PLC controller; 9. Matching 110V power supply; 10. First two-axis stepper motor controller; 11. Second two-axis stepper motor controller; 12. Electronic eyepiece; 13. Microscope body; 14. Microscope support; 15. Mounting bracket; 16. Air blower; 17. Fiber optic sensor. Detailed Implementation

[0027] The embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of the invention.

[0028] Please see Figure 1 - Figure 6 The present invention provides a PLC-based intelligent optical microscope stage, including a mechanical support module, a two-dimensional motion module, a control drive module and an image acquisition adapter module.

[0029] The mechanical support module is used to support the two-dimensional motion module, the control drive module, and the image acquisition adapter module. The mechanical support module includes an ABS honeycomb mounting plate 1 and a cylindrical support frame 2. The ABS honeycomb mounting plate 1 has a size of 370mm×270mm and there are 2 pieces. The cylindrical support frame 2 is a 3D printed connecting column made of PLA, with 7 pieces and a height of 8cm, used to reinforce the connection between the upper ABS honeycomb mounting plate 1 and the lower mounting surface.

[0030] The double-layer support structure, consisting of an ABS honeycomb mounting plate 1 and a cylindrical support frame 2, with the cylindrical support frame 2 being 3D-printed connecting columns evenly distributed at the bottom edge of the upper mounting plate, along with 3D-printed PLC controller brackets, protective sleeves for the electronic eyepiece 12, and other compatible components, achieves both lightweight and high rigidity. This effectively isolates vibrations and ensures stable cooperation between the microscope body 13, the electronic eyepiece 12, and the stage. The structure is robust and highly adaptable.

[0031] The two-dimensional motion module includes a first SGX ball screw single-track slide 5, a second SGX ball screw single-track slide 7, a four-hole cross connector 6, a Z-axis lifting platform 4, and a slide stage 3. Both the first SGX ball screw single-track slide 5 and the second SGX ball screw single-track slide 7 are SGX series ball screw single-track slides with a stroke of 250mm and a repeatability of ±0.01mm. The first SGX ball screw single-track slide 5 and the second SGX ball screw single-track slide 7 are orthogonally connected by the four-hole cross connector 6 to form an XY-axis motion platform. The four-hole cross connector 6 is a component of the SGX slides. The slide stage 3 is made of aluminum alloy and has four M4 screw holes on its upper and lower surfaces. It is fixed above the XY axis motion platform by a Z-axis lifting platform 4. The Z-axis lifting platform 4 is a manual Z-axis lifting platform with a stroke of 20mm and an adjustment accuracy of 0.01mm. The slide stage 3 is a 3D printed part made of PLA and is sized to fit standard slides. The slide stage 3 has a positioning structure on its inner side that is compatible with the slides. The positioning structure is a micro-sinking design with a depth of 0.2mm. The slide stage 3 has a gripping opening on its edge for easy loading and unloading of slides. The gripping opening is a semi-rounded corner structure with a radius of 6mm.

[0032] The PLC controller 8 presets the XY axis motion path and stepping parameters, and the fiber optic sensor 17 automatically detects whether a glass slide is placed in the positioning structure of the glass slide stage 3. The air blower 16 blows air to clean the surface of the glass slide during the image acquisition interval, so that there is no need for repeated manual adjustment and recording. The sample full-area scanning and image acquisition can be completed automatically according to the preset path. After the acquisition is completed, the XY axis motion platform automatically returns to the initial position, which greatly simplifies the operation process and improves the efficiency of panoramic image acquisition.

[0033] The control and drive module controls the movement of the two-dimensional motion module. The control and drive module includes a PLC controller 8, a first two-axis stepper motor controller 10, a second two-axis stepper motor controller 11, and a matching 110V power supply 9. The PLC controller 8 is an FBs series controller. The first two-axis stepper motor controller 10 and the second two-axis stepper motor controller 11 are both SGX slide table controllers. The PLC controller 8 is electrically connected to the first two-axis stepper motor controller 10 and the second two-axis stepper motor controller 11. The first two-axis stepper motor controller 10 is connected to the second two-axis stepper motor... The controller 11 is electrically connected to the stepper motors in the first SGX ball screw single linear guide slide 5 and the second SGX ball screw single linear guide slide 7, respectively. The matching 110V power supply 9 is a 110V DC power supply with an output power of 500W, which powers the control drive module to realize the stepper control of the two-dimensional motion module. The control drive module also includes a bracket for the PLC controller 8. The bracket for the PLC controller 8 is a 3D printed part made of PLA and has a snap-fit ​​structure that is compatible with the PLC controller 8. The PLC controller 8 is fixed to the ABS honeycomb mounting plate 1 by the bracket.

[0034] The PLC controller 8, in conjunction with the first two-axis stepper motor controller 10 and the second two-axis stepper motor controller 11, drives the stepper motors in the first SGX ball screw single-track slide 5 and the second SGX ball screw single-track slide 7 to operate. The first SGX ball screw single-track slide 5 and the second SGX ball screw single-track slide 7 are orthogonally fixed together by the four-hole cross connector 6 to form an XY axis motion platform, thereby realizing the automated and precise stepping movement of the glass slide in the XY axis direction. The step distance is consistent and the repeatability can reach ±0.01mm, effectively solving the problem of insufficient precision of traditional manual stage.

[0035] The two-dimensional motion module can move the slide stage 3 to perform microscopic observation in conjunction with the image acquisition adapter module. The image acquisition adapter module includes a microscope body 13, a microscope support 14, and an electronic eyepiece 12. The microscope support 14 is made of cast iron and is 337mm high. It is fixed on the ABS honeycomb mounting plate 1 and is used to support the microscope body 13 and align the objective lens with the slide stage 3. The microscope body 13 is a 400x optical microscope, and the electronic eyepiece 12 is a 10x electronic eyepiece used to acquire electronic images. The image acquisition adapter module also includes a protective cover for the electronic eyepiece 12. The protective cover for the electronic eyepiece 12 is a 3D printed part with an inner diameter that matches the electronic eyepiece 12. It is made of PLA and serves as a protective and anti-shake function.

[0036] Image collection is accomplished by setting up a control drive module and a two-dimensional motion module. High-precision automated positioning reduces the overlap and omission of images in adjacent areas, providing high-quality raw data for subsequent panoramic image stitching. Combined with SIFT feature matching algorithm for duplicate area detection, dynamic erosion optimization module for regional non-uniform erosion processing, and Poisson fusion technology to eliminate seam artifacts, stitching errors are effectively reduced, ensuring natural transition of cell edges and no distortion of cell nucleus morphology, meeting the precise needs of pathological observation and scientific research analysis.

[0037] A mounting bracket 15 is fixedly installed on the side wall of the microscope body 13. Two sets of air blowers 16 and fiber optic sensors 17 are respectively provided on the inner side of the mounting bracket 15. Each set of air blowers 16 and fiber optic sensors 17 are parallel to each other. The fiber optic sensors 17 are used to detect whether there is a slide on the positioning structure of the slide stage 3. The distance between each set of air blowers 16 and fiber optic sensors 17 is the distance between the positioning structures on the slide stage 3.

[0038] By fixing a mounting bracket 15 to the side wall of the microscope body 13, two sets of air blowers 16 and fiber optic sensors 17 are respectively set on the inner side of the mounting bracket 15. Each set of air blowers 16 and fiber optic sensors 17 are parallel to each other, and the spacing is adapted to the spacing between the positioning structures on the slide stage 3, thereby realizing multi-station synchronous detection and cleaning. The fiber optic sensor 17 can automatically detect whether a slide is placed in the positioning structure of the slide stage 3, ensuring accurate triggering of the subsequent image acquisition process. The air blower 16 can blow air to clean the surface of the slide, remove floating dust to ensure image clarity, and improve detection efficiency and image quality.

[0039] Working principle:

[0040] Step 1: Initialization and Slide Detection Stage. The operator places the biological tissue sample slide into the micro-sinking positioning structure of the slide stage 3. By rotating the adjustment knob of the Z-axis lifting platform 4, the vertical height of the slide stage 3 is finely adjusted so that the sample is at the optimal imaging focal plane of the microscope body 13. The mounting bracket 15 is fixed on the side wall of the microscope body 13. The fiber optic sensor 17 is located inside the mounting bracket 15 to detect whether a slide is placed in the positioning structure of the slide stage 3, ensuring accurate triggering of the subsequent image acquisition process. Each set of air blowers 16 and fiber optic sensors 17 are parallel to each other, and the spacing is adapted to the spacing between the positioning structures on the slide stage 3, realizing multi-station synchronous detection. The slide can also be cleaned by turning on the air pump.

[0041] Step 2: PLC-driven automated positioning and movement stage. The PLC controller 8 presets the XY axis motion path and stepping parameters, and the matching 110V power supply 9 supplies power to the control drive module. The PLC controller 8 sends pulse signals to the first two-axis stepper motor controller 10 and the second two-axis stepper motor controller 11. The first two-axis stepper motor controller 10 and the second two-axis stepper motor controller 11 respectively drive the stepper motors in the first SGX ball screw single linear rail slide 5 and the second SGX ball screw single linear rail slide 7 to operate. The XY axis motion platform formed by the first SGX ball screw single linear rail slide 5 and the second SGX ball screw single linear rail slide 7 orthogonally fixed through the four-hole cross connector 6 moves area by area according to the preset path, realizing the two-dimensional precise displacement of the slide stage 3. The repeatability positioning accuracy can reach ±0.01mm, and the stroke covers a range of 250mm.

[0042] Step 3: Image acquisition and cleaning phase for each area. Each time the PLC controller 8 moves to a preset position, it sends a trigger signal to the electronic eyepiece 12. The electronic eyepiece 12 captures the sample image of the current area. A protective sleeve is fitted over the electronic eyepiece 12 to provide protection and prevent shaking, ensuring the stability of the acquired image. An air blower 16 is located inside the mounting bracket 15 and blows air onto the slide surface during image acquisition intervals to remove dust and ensure image clarity. After acquiring images of all preset areas, the XY-axis motion platform automatically returns to its initial position, ending the acquisition process. This phase achieves automated and precise positioning of the slide and full-area image acquisition, offering convenient operation and high positioning accuracy.

[0043] Step 4:

[0044] 1. After the computer drives the stage to acquire adjacent field-of-view image sequences through the PLC motion control system, the SIFT feature matching algorithm is started to detect repeated regions. During the feature point matching process, the algorithm not only calculates the pixel coordinate correspondence of the overlapping regions of the two images, but also extracts the pixel similarity distribution data and edge continuity variance of the overlapping regions simultaneously. Based on this, the system runs the dynamic erosion optimization module.

[0045] 2. Statistical calculation model:

[0046] Corrosion percentage C=

[0047] Where δ0=1.5 (benchmark variance) and σ0=0.1 (benchmark standard deviation), this formula dynamically generates the optimal erosion rate by weighted fusion of edge continuity and pixel similarity;

[0048] 3. Corrosion execution mechanism: The system inputs the calculated C value into the image processing circuit and performs regional corrosion on the overlapping area of ​​Figure B—corroding 85% of the width along the X-axis and 87% of the height along the Y-axis to ensure a natural transition between the corrosion boundary and the cell edge. The corrosion process adopts a non-uniform corrosion strategy: the corrosion rate is increased to 87% for uniformly stained areas and reduced to 83% for densely celled areas to avoid distortion of cell nucleus morphology.

[0049] 4. Stitching Stage: The system uses an image fusion algorithm to weightedly superimpose the eroded image A and image B: the weight allocation for the overlapping area is as follows:

[0050]

[0051] Combine Poisson fusion technology to eliminate seam artifacts.

[0052] The embodiments of the present invention are given for illustrative and descriptive purposes only, and are not intended to be exhaustive or to limit the invention to the forms disclosed. Many modifications and variations will be apparent to those skilled in the art. The embodiments were chosen and described to better illustrate the principles and practical application of the invention, and to enable those skilled in the art to understand the invention and design various embodiments with various modifications suitable for a particular purpose.

Claims

1. A PLC-based intelligent optical microscope stage, comprising a mechanical support module, a two-dimensional motion module, a control drive module, and an image acquisition adapter module, characterized in that: The mechanical support module is used to support the two-dimensional motion module, the control drive module and the image acquisition adapter module. The mechanical support module includes an ABS honeycomb mounting plate (1) and a cylindrical support frame (2). The cylindrical support frame (2) is a 3D printed connecting column used to reinforce the connection between the ABS honeycomb mounting plate (1) and the mounting surface. The two-dimensional motion module includes a first SGX ball screw single-rail slide (5), a second SGX ball screw single-rail slide (7), a four-hole cross connector (6), a z-axis lifting platform (4), and a glass slide stage (3). The two-dimensional motion module can move the slide stage (3) and cooperate with the image acquisition adapter module for microscopic observation; The control and drive module can control the movement of the two-dimensional motion module.

2. The PLC-based intelligent optical microscope stage as described in claim 1, characterized in that, The first SGX ball screw single-rail slide (5) and the second SGX ball screw single-rail slide (7) are orthogonally connected by a four-hole cross connector (6) to form an XY axis motion platform.

3. The PLC-based intelligent optical microscope stage as described in claim 1, characterized in that, The slide stage (3) is fixed above the XY axis motion platform by the z-axis lifting platform (4), and the slide stage (3) has a positioning structure that is adapted to the slide on its inner side. The slide stage (3) is a 3D printed part, and its positioning structure is a micro-sinking design, and the edge is provided with a gripping port for easy picking up and putting down the slide.

4. The PLC-based intelligent optical microscope stage as described in any one of claims 1-3, characterized in that, The control drive module includes a PLC controller (8), a first two-axis stepper motor controller (10), and a matching 110V power supply (9). The PLC controller (8) is electrically connected to the first two-axis stepper motor controller (10) and the second two-axis stepper motor controller (11). The first two-axis stepper motor controller (10) and the second two-axis stepper motor controller (11) are electrically connected to the stepper motors in the first SGX ball screw single linear guide slide (5) and the second SGX ball screw single linear guide slide (7), respectively.

5. The PLC-based intelligent optical microscope stage as described in claim 1, characterized in that, The matching 110V power supply (9) supplies power to the control drive module to realize the step control of the two-dimensional motion module. The control drive module also includes a 3D printed PLC controller (8) bracket, which is fixed on the ABS honeycomb mounting plate (1) by the bracket.

6. The PLC-based intelligent optical microscope stage as described in claim 1, characterized in that, The image acquisition adapter module includes a microscope body (13), a microscope support (14), and an electronic eyepiece (12).

7. The PLC-based intelligent optical microscope stage as described in claim 6, characterized in that, The microscope support (14) is fixed on the ABS honeycomb mounting plate (1).

8. The PLC-based intelligent optical microscope stage as described in claim 7, characterized in that, The ABS honeycomb mounting plate (1) is used to support the microscope body (13) and make the objective lens correspond to the slide stage (3), and the electronic eyepiece (12) is used to acquire electronic images.

9. The PLC-based intelligent optical microscope stage as described in claim 8, characterized in that, A mounting bracket (15) is fixedly installed on the side wall of the microscope body (13). Two sets of air blowing heads (16) and fiber optic sensors (17) are respectively provided on the inner side of the mounting bracket (15).

10. The PLC-based intelligent optical microscope stage as described in claim 9, characterized in that, Each set of air blowers (16) and fiber optic sensors (17) are parallel to each other. The fiber optic sensors (17) are used to detect whether there is a glass slide on the positioning structure of the slide stage (3). The distance between each set of air blowers (16) and fiber optic sensors (17) is the distance between the positioning structures on the slide stage (3).

Images