A skin care and skin smoothing composition containing DNA sodium, its preparation process and application

Abstract

The application relates to a skin repairing and smoothing composition containing DNA sodium, a preparation process and application, and belongs to the technical field of cosmetics. The composition is prepared from the following raw materials: DNA sodium, hydrolyzed sodium hyaluronate, alpha-glucan oligosaccharide, ikkduin, plant-derived ingredients, polyhydric alcohol and water; the plant-derived ingredients include Yunnan paris extract and white water lily extract in a weight ratio of 1:(0.5-1.5), and further include mulberry root extract; the mulberry root extract is obtained by transferring the obtained adventitious roots to a liquid culture medium containing L-phenylalanine and L-tyrosine for further culture, and then extracting with 85-95 wt% ethanol. The application optimizes the types and proportions of raw materials and the preparation process, and endows the composition with excellent skin repairing, smoothing and whitening effects.

Description

Technical Field

[0001] This invention specifically relates to a skin repair and skin-evening composition containing sodium DNA, its preparation process, and its application, falling under the category of cosmetic technology. Background Technology

[0002] Inflammatory skin responses are a core factor contributing to various skin problems: excessive release of inflammatory factors (such as TNF-α) can damage the skin barrier function, leading to discomfort such as sensitivity, redness, and dryness. Simultaneously, inflammatory signals can stimulate melanocyte activation, exacerbating pigmentation and uneven skin tone. Therefore, developing cosmetic ingredients and compositions that combine anti-inflammatory, repairing, and whitening / even-toning effects has become a hot research topic in the industry.

[0003] Currently, some products on the market claim to have both repair and whitening effects, but they have some shortcomings: First, the screening and compounding of active ingredients lack systematic design and research, and different active ingredients are prone to antagonistic effects or mutual dilution of their effects, resulting in actual skin care effects that are not as expected; second, the preparation methods of plant extracts are not compatible with plant ingredients, and the content of active ingredients is unstable, making it difficult to meet the needs of high-efficacy products; third, existing compositions often require the addition of high concentrations of active ingredients to achieve the ideal repair or whitening effect, which increases product costs and also increases the technical difficulty of formula safety and compatibility.

[0004] Therefore, how to improve repair and whitening effects through reasonable raw material selection, specific preparation processes, and scientific compounding ratios remains a technical problem that urgently needs to be solved. Summary of the Invention

[0005] To address the technical problems mentioned in the background art, the present invention provides a skin repair and skin-tone-clearing composition containing sodium DNA, made from the following raw materials: sodium DNA, hydrolyzed sodium hyaluronate, α-glucan oligosaccharide, ectoine (also known as tetrahydromethylpyrimidine carboxylic acid), plant-derived ingredients, polyols, and water.

[0006] Furthermore, the plant-derived components include Yunnan Paris extract and white water lily extract in a weight ratio of 1:(0.5-1.5), as well as mulberry root extract.

[0007] Furthermore, the preparation process of mulberry root extract includes: taking young mulberry roots, processing them, and inoculating them into a culture medium to obtain callus tissue. The callus tissue is cultured to produce adventitious roots. The adventitious roots are transferred to a liquid culture medium containing L-phenylalanine and L-tyrosine for further culture. The adventitious roots obtained from further culture are added to 85-95wt% ethanol to form a pulp, heated for extraction, centrifuged, and the centrifuged supernatant is concentrated and dried to obtain the final product.

[0008] Further, the composition is made from the following raw materials by weight percentage: 0.001-0.5% sodium DNA, 0.01-1.5% hydrolyzed sodium hyaluronate, 1-3% α-glucan oligosaccharide, 0.001-0.25% ectoine, 2-6% plant-derived ingredients, 5-25% polyols and the balance water.

[0009] Furthermore, the amount of sodium DNA used is 0.005-0.2%, 0.01-0.2%, or 0.05-0.15%.

[0010] Furthermore, the amount of hydrolyzed sodium hyaluronate used is 0.02-1%, 0.05-0.95%, or 0.08-0.8%.

[0011] Furthermore, the amount of α-glucan oligosaccharides used is 1.2-2.5%, 1.5-2.4%, or 1.8-2.2%.

[0012] Furthermore, the dosage of ectoine is 0.005-0.25%, 0.01-0.2%, or 0.02-0.15%.

[0013] Furthermore, the amount of plant-derived ingredients used is 2.2-5.8%, 2.5-5.5%, or 3-5.5%.

[0014] Furthermore, the amount of polyol used is 8-25%, 9-22%, or 10-20%.

[0015] Furthermore, the polyol includes at least one of ethylene glycol, propylene glycol, butylene glycol, glycerol, polyethylene glycol, and pentanediol.

[0016] Furthermore, the plant-derived components are in a weight ratio of 1:(0.8-1.2):(0.2-0.7):(0.5-1.5) extracts of Paris polyphylla, mulberry root, ginger root, and white water lily.

[0017] Furthermore, the extracts of Paris polyphylla, ginger root, and white water lily were obtained by extraction with 65-85 wt% ethanol.

[0018] Furthermore, the preparation process of Yunnan Paris extract includes: adding Yunnan Paris into 65-85wt% ethanol and slurrying, heating and extracting at 50-65℃ for 1-5 hours, centrifuging, taking the supernatant, and obtaining it by concentration and drying.

[0019] Furthermore, the preparation process of ginger root extract includes: adding ginger root to 65-85wt% ethanol to make a pulp, heating and extracting at 50-65℃ for 1-5 hours, centrifuging, taking the supernatant, and obtaining it by concentration and drying.

[0020] Furthermore, the preparation process of white water lily extract includes: adding white water lily to 65-85wt% ethanol and slurrying, heating and extracting at 50-65℃ for 1-5 hours, centrifuging, taking the supernatant, and obtaining it by concentration and drying.

[0021] Furthermore, in the preparation process of mulberry root extract, the culture medium used for callus culture is prepared with MS medium, and 0.2-0.8 mg / L 2,4-dichlorophenoxyacetic acid, 0.3-0.8 mg / L α-naphthaleneacetic acid, 0.3-0.8 mg / L 6-benzylaminopurine, 20-40 g / L sucrose and 5-10 g / L agar are added.

[0022] Furthermore, in the preparation process of mulberry root extract, the culture medium used for the adventitious root production from callus tissue culture is prepared with 1 / 2 MS medium, and 0.8-1.5 mg / L indolebutyric acid and 20-40 g / L sucrose are added.

[0023] Furthermore, in the preparation process of mulberry root extract, the liquid culture medium is prepared as 1 / 2 MS medium, and 5-20 mg / L L-phenylalanine, 5-20 mg / L L-tyrosine, 0.8-1.5 mg / L indolebutyric acid and 20-40 g / L sucrose are added.

[0024] Furthermore, in the preparation process of mulberry root extract, the culture time for callus tissue is 20-30 days; the culture time for adventitious roots to be generated from callus tissue is 20-30 days; and the culture time in liquid culture medium is 7-15 days. The culture temperature is 20-30℃.

[0025] Furthermore, in the preparation process of mulberry root extract, the weight ratio of L-phenylalanine to L-tyrosine is 1:1-2.

[0026] Furthermore, in the preparation process of mulberry root extract, the temperature and time for ethanol extraction are 40-65℃ and 0.5-5 hours.

[0027] The present invention also provides a preparation process for a skin repair and skin-evening composition containing sodium DNA, including a step of mixing the raw materials.

[0028] The present invention also provides the application of the aforementioned skin repair and skin-evening composition containing sodium DNA in the preparation of moisturizing, soothing, and whitening cosmetics.

[0029] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention scientifically combines sodium DNA, hydrolyzed sodium hyaluronate, α-glucan oligosaccharide, ectoine, and specific plant-derived ingredients (Yunnan Paris polyphylla extract, mulberry root extract, white water lily extract, and ginger root extract) to obtain a composition with excellent repair and whitening effects, which has good application prospects in cosmetics.

[0030] This invention utilizes specific plant tissue culture techniques to prepare mulberry root extract. The process involves "callus → adventitious roots → enhanced culture in a medium containing L-phenylalanine and L-tyrosine." By simultaneously using L-phenylalanine and L-tyrosine in the culture medium with an optimized weight ratio of 1:1-2, the accumulation of tyrosinase-inhibiting active ingredients in the mulberry root extract is significantly increased. Furthermore, the simultaneous use of L-phenylalanine and L-tyrosine in the culture medium synergistically enhances the accumulation of tyrosinase-inhibiting active substances in the extract, thereby improving its whitening effect.

[0031] This invention optimizes the raw material composition by combining Yunnan Paris polyphylla extract and white water lily extract and studying the effect of their compound ratio on the skin repair and soothing effects. The study screened out that the synergistic effect is achieved when the weight ratio of the two is 1:0.5-1.5, providing relevant theoretical support for the development of high-performance cosmetics.

[0032] Furthermore, the composition of this invention can achieve significant whitening and repairing effects at low concentrations, and the main active ingredients are all raw materials recognized as safe in the cosmetics field, with good compatibility stability and application prospects. It can be directly applied to cosmetics with various functions such as moisturizing, soothing and repairing the skin, and whitening. Detailed Implementation

[0033] The present invention will be further explained below with reference to implementation examples and comparative examples.

[0034] Example 1 Mulberry root extract (1-a): Young roots of mulberry (Morus ALBA) were taken, rinsed with water, soaked in 75wt% ethanol for 30 seconds for disinfection, rinsed with water, cut into 1cm segments, and inoculated into culture medium 1. Callus tissue was generated after culturing at 25℃ for 25 days. The callus tissue was transferred to culture medium 2 and cultured at 25℃ for 25 days to generate adventitious roots. The adventitious roots were transferred to culture medium 3 and cultured at 25℃ for 12 days. The adventitious roots obtained from the culture were taken out and 15 times their weight of 90wt% ethanol was added. The mixture was pulped, heated to 58℃ and stirred for 3.5 hours. The mixture was centrifuged at 4000rpm for 25 minutes. The supernatant was collected, concentrated to remove the ethanol, and then freeze-dried to a water content of 3.95wt%. Culture medium 1 was prepared using MS medium (Solarbio, catalog number M8521, Beijing Solarbio Technology Co., Ltd.). Specifically, 4.74 g of MS medium was added to 1 L of water, along with the following ingredients: 0.5 mg / L 2,4-dichlorophenoxyacetic acid, 0.5 mg / L α-naphthaleneacetic acid, 0.5 mg / L 6-benzylaminopurine, 30 g / L sucrose, and 7 g / L agar. The mixture was then sterilized. Culture medium 2: Prepared using 1 / 2MS medium (Solarbio, catalog number M8526, Beijing Solarbio Technology Co., Ltd.), specifically by adding 4g of 1 / 2MS medium to 1L of water, adding the following ingredients: 1mg / L indolebutyric acid and 30g / L sucrose, and then sterilizing.

[0035] Culture medium 3: Prepared using 1 / 2 MS medium (Solarbio, catalog number M8526, Beijing Solarbio Technology Co., Ltd.), specifically: 4g of 1 / 2 MS medium was added to 1L of water, along with the following ingredients: 15mg / L L-phenylalanine, 15mg / L L-tyrosine, 1mg / L indolebutyric acid, and 30g / L sucrose, followed by sterilization. The weight ratio of L-phenylalanine to L-tyrosine was 1:1.

[0036] Mulberry root extract (1-b): Young roots of mulberry (Morus ALBA) were taken, rinsed with water, soaked in 75wt% ethanol for 30 seconds for disinfection, rinsed with water, cut into 1cm segments, and inoculated into culture medium 1. Callus tissue was generated after culturing at 27℃ for 23 days. The callus tissue was transferred to culture medium 2 and cultured at 27℃ for 24 days to generate adventitious roots. The adventitious roots were transferred to culture medium 3 and cultured at 27℃ for 13 days. The adventitious roots obtained from the culture were taken out and 15 times their weight of 90wt% ethanol was added. The mixture was pulped, heated to 58℃ and stirred for 3.5 hours. The mixture was centrifuged at 4000rpm for 25 minutes. The supernatant was collected, concentrated to remove the ethanol, and then freeze-dried to a water content of 3.95wt%. Culture medium 1 was prepared using MS medium (Solarbio, catalog number M8521, Beijing Solarbio Technology Co., Ltd.). Specifically, 4.74 g of MS medium was added to 1 L of water, and the following ingredients were added: 0.65 mg / L 2,4-dichlorophenoxyacetic acid, 0.55 mg / L α-naphthaleneacetic acid, 0.45 mg / L 6-benzylaminopurine, 32 g / L sucrose, and 7 g / L agar. The mixture was then sterilized. Culture medium 2: Prepared using 1 / 2MS medium (Solarbio, catalog number M8526, Beijing Solarbio Technology Co., Ltd.), specifically: 4g of 1 / 2MS medium was added to 1L of water, and the following raw materials were added: 1.1mg / L indolebutyric acid and 28g / L sucrose, followed by sterilization.

[0037] Culture medium 3: Prepared using 1 / 2 MS medium (Solarbio, catalog number M8526, Beijing Solarbio Technology Co., Ltd.), specifically: 4g of 1 / 2 MS medium was added to 1L of water, along with the following ingredients: 9.8mg / L L-phenylalanine, 19.6mg / L L-tyrosine, 0.95mg / L indolebutyric acid, and 30g / L sucrose, followed by sterilization. The weight ratio of L-phenylalanine to L-tyrosine was 1:2.

[0038] Mulberry root extract (1-c): The difference between this and mulberry root extract (1-a) is that 15 mg / L L-phenylalanine and 15 mg / L L-tyrosine in culture medium 3 were replaced with 30 mg / L L-phenylalanine.

[0039] Mulberry root extract (1-d): The difference between this and mulberry root extract (1-a) is that 15 mg / L L-phenylalanine and 15 mg / L L-tyrosine in culture medium 3 were replaced with 30 mg / L L-tyrosine.

[0040] Mulberry root extract (1-e): The difference between this and mulberry root extract (1-a) is as follows: In culture medium 3, 15 mg / L L-phenylalanine and 15 mg / L L-tyrosine are replaced with 20 mg / L L-phenylalanine and 10 mg / L L-tyrosine, with the weight ratio of L-phenylalanine to L-tyrosine being 1:0.5.

[0041] Mulberry root extract (1-f): The difference between this and mulberry root extract (1-a) is as follows: In culture medium 3, 6 mg / L L-phenylalanine and 24 mg / L L-tyrosine are replaced with 20 mg / L L-phenylalanine and 10 mg / L L-tyrosine, with the weight ratio of L-phenylalanine to L-tyrosine being 1:4.

[0042] Mulberry root extract (1 g): The difference between this and mulberry root extract (1-a) is as follows: In culture medium 3, 3.75 mg / L L-phenylalanine and 26.5 mg / L L-tyrosine were replaced with 20 mg / L L-phenylalanine and 10 mg / L L-tyrosine, with the weight ratio of L-phenylalanine to L-tyrosine being 1:7.

[0043] Mulberry root extract (1-h): The difference between this and mulberry root extract (1-a) is that 15 mg / L L-phenylalanine and 15 mg / L L-tyrosine were removed from culture medium 3.

[0044] Mulberry root extract (1-i): The difference from mulberry root extract (1-a) is that it is extracted directly with ethanol solution without cultivation. Specifically, young roots of mulberry (MORUS ALBA) are taken, 15 times their weight of 90wt% ethanol is added, pulped, heated to 58℃ and stirred for 3.5 hours, centrifuged at 4000rpm for 25 minutes, the supernatant is concentrated to remove ethanol, and then freeze-dried to a water content of 3.95wt%.

[0045] The whitening effects of the above extracts were tested.

[0046] Test Principle and Purpose: Human skin color is related to the types and amounts of pigments present in the skin. Melanin has the greatest impact on skin color and is formed in melanocytes in the basal layer of the epidermis. The process involves tyrosine in melanocytes being catalyzed by tyrosinase to ultimately form melanin. Therefore, tyrosinase is a key enzyme in melanin production, controlling the formation process, and its activity level plays a major role in melanin deposition in the skin. Therefore, testing the inhibition rate of the test sample against tyrosinase can assess the inhibitory effect of the test sample on tyrosinase activity. The whitening efficacy of the test product is evaluated by detecting its tyrosinase inhibition rate.

[0047] Instruments and reagents: Microplate reader (BioTek), analytical balance (Shimadzu), pipette (Beijing Dalong), disodium hydrogen phosphate-citrate buffer (pH 7.0), tyrosinase, L-tyrosine.

[0048] Test method: Mulberry root extract (1-a to 1-i), tyrosinase, and L-tyrosine were diluted with disodium hydrogen phosphate-citrate buffer to obtain test solutions (1 mg / mL), tyrosinase solutions (100 U / mL), and tyrosine solutions (0.5 mg / mL). The following reaction mixtures were prepared for each test: A0 group: 100 μL tyrosine solution, 50 μL buffer solution, and 50 μL test solution; A1 group: 100 μL tyrosine solution, 50 μL tyrosinase solution, and 50 μL test solution; T0 group: 100 μL tyrosine solution and 100 μL buffer solution; T1 group: 100 μL tyrosine solution, 50 μL tyrosinase solution, and +50 μL buffer solution. Each reaction mixture was reacted at room temperature for 30 min. After the reaction, the absorbance (OD) value was measured at 475 nm. The test was performed in triplicate, and the tyrosinase inhibition rate was calculated. Tyrosinase inhibition rate = (1 - (OD value of group A1 - OD value of group A0) / (OD value of group T1 - OD value of group T0)) × 100%. The test results are shown in Table 1.

[0049] Table 1: Results of Tyrosinase Inhibition Rate Test

[0050] Example 1 investigated the inhibitory activity of mulberry root extracts (1-a to 1-i) obtained under different culture conditions and extraction methods on tyrosinase activity to evaluate their whitening efficacy. The results showed that all tested samples exhibited some tyrosinase inhibitory activity, but the activity differed significantly among different samples. Among them, samples 1-a and 1-b, induced by callus-adventitious root-enhanced culture medium, showed the best activity, with inhibition rates of 88.14% and 86.75%, respectively, indicating that the tissue culture technology of this invention can effectively enrich the tyrosinase-inhibiting active components in mulberry roots. Comparing the results of 1-a and 1-c to 1-h, it can be seen that the combination and ratio of L-phenylalanine and L-tyrosine in culture medium 3 have a significant impact on the inhibition of tyrosinase activity. The extract with a weight ratio of 1:1-2 shows high activity, while the activity of adding phenylalanine alone (1-c) or tyrosine alone (1-d) decreases to 72.09% and 70.58%, respectively. In the culture medium omitting both (1-h), the activity is only 59.16%, indicating that the synergistic effect of L-phenylalanine and L-tyrosine is necessary to significantly enhance the activity of the active ingredient. Furthermore, when the ratio of L-phenylalanine to L-tyrosine is not within the 1:1-2 range (1-e: 1:0.5, activity 79.07%; 1-f: 1:4, activity 81.34%; 1-g: 1:7, activity 77.63%), the activity shows a decreasing trend, confirming that using a weight ratio of 1:1-2 of L-phenylalanine and L-tyrosine in the culture medium of this invention results in better extract activity. The wild mulberry root extract (1-i) obtained by direct alcohol extraction without tissue culture had the lowest activity (52.27%), which was only 59.3% of that of 1-a. This indicates that the specific tissue culture process used in this invention has a significant enrichment effect on the whitening active ingredients of mulberry root.

[0051] Example 2 Yunnan Paris polyphylla extract: The rhizome of Yunnan Paris polyphylla (PARIS POLYPHYLLA YUNNANENSIS) was mixed with 15 times its weight of 75wt% ethanol solution and stirred at 60℃ for 2 hours. After centrifugation at 3000rpm for 25 minutes, the supernatant was collected, concentrated under reduced pressure to remove ethanol, and freeze-dried to a water content of 3.84wt%.

[0052] White water lily extract: Take the leaves of white water lily (NYMPHAEA ALBA), add 16.5 times its weight of 75wt% ethanol solution and mix. Stir and extract at 60℃ for 2 hours, centrifuge at 3000rpm for 20 minutes, collect the clear liquid and concentrate under reduced pressure to remove ethanol, and freeze dry to a water content of 3.72wt%.

[0053] The sample to be tested was prepared, and its composition is as follows: 2-a: Extract from Paris polyphylla yunnanensis.

[0054] 2-b: White water lily extract.

[0055] 2-c: The weight ratio of Yunnan Paris extract and white water lily extract is 1:0.05.

[0056] 2-d: The weight ratio of Yunnan Paris extract to white water lily extract is 1:0.2.

[0057] 2-e: The weight ratio of Yunnan Paris extract to white water lily extract is 1:0.5.

[0058] 2-f: The weight ratio of Yunnan Paris extract to white water lily extract is 1:0.9.

[0059] 2-g: Yunnan Paris extract and white water lily extract in a weight ratio of 1:1.5.

[0060] 2-h: The weight ratio of Yunnan Paris extract and white water lily extract is 1:3.

[0061] 2-i: The weight ratio of Yunnan Paris extract and white water lily extract is 1:5.

[0062] 2-j: The weight ratio of Yunnan Paris extract and white water lily extract is 1:10.

[0063] The skin repair effect was tested on the test samples 2-a to 2-j above.

[0064] Test Principle and Purpose: By comparing the differences in TNF-α secretion levels in RAW264.7 cells after administration of the test substance, the ability of the test substance to inhibit the secretion of cellular inflammatory factors is evaluated. The skin-repairing efficacy of the test product is assessed by detecting its ability to inhibit inflammatory factors.

[0065] Instruments and reagents: RAW264.7 (log phase), multi-functional enzyme-linked immunosorbent assay (ELISA) analyzer, analytical balance (Shimadzu), pipette (Beijing Dalong), disodium hydrogen phosphate-citrate buffer (pH 7.0), TNF-α assay kit, lipopolysaccharide (LPS).

[0066] Test method: Adjust the RAW264.7 cell concentration with culture medium, add to 96-well plates at a density of 200 μL per well and 2 × 10⁶ cells per well. 4The samples were inoculated. After incubation at 37℃ and 5% CO2 for 24 hours, the culture medium was discarded. 200 μL of fresh culture medium was added to the blank group, and 200 μL of fresh culture medium containing 0.5 μg / mL LPS was added to the model group. 200 μL of fresh culture medium containing 0.5 μg / mL LPS and 0.5 mg / mL of sample 2-a to 2-j was added to the test groups (corresponding to samples 2-a to 2-j). Five replicates were set up. The samples were incubated at 37℃ and 5% CO2 for 24 hours. After incubation, the TNF-α content was measured according to the instructions of the TNF-α enzyme-linked immunosorbent assay kit. The absorbance of each well was measured at 450 nm using a microplate reader to obtain the TNF-α content. The TNF-α inhibition rate was calculated based on the TNF-α content. The results are shown in Table 2.

[0067] Table 2: TNF-α content and inhibition rate

[0068] In Table 2: Compared with the model group: "*": p < 0.01; "**": p < 0.01; "***": p < 0.001.

[0069] Example 2 investigated the inhibitory effects of *Paris polyphylla* extract and *Nymphaea leucocephala* extract in different ratios (2-a to 2-j) on LPS-induced TNF-α secretion from RAW264.7 cells to evaluate their anti-inflammatory efficacy in repairing the skin. The results showed that when *Paris polyphylla* extract (2-a) or *Nymphaea leucocephala* extract (2-b) were used alone, the TNF-α inhibition rates were 23.68% and 26.79%, respectively, indicating good inhibitory effects when used alone. However, when the two were combined, the inhibition rate initially increased and then decreased with increasing proportion of *Nymphaea leucocephala* extract: within the weight ratio of *Paris polyphylla* to *Nymphaea leucocephala* from 1:0.05 to 1:0.9, the inhibition rate gradually increased from 22.84% to 39.65% (2-f, optimal ratio); but when the ratio continued to increase to 1:1.5, 1:3, 1:5, and 1:10, the inhibition rate gradually decreased to 37.06%, 32.48%, 28.31%, and 24.76%, respectively. The above results indicate that the two ingredients have a relatively weak antagonistic effect at a ratio of 1:0.05, while the combination effect is additive at ratios of 1:5 and 1:10. A strong synergistic effect is observed at ratios in the range of 1:0.5-1.5, significantly enhancing the ability to inhibit inflammatory factors, with the optimal ratio being 1:0.9. The specific compound ratio of *Paris polyphylla* and *Nymphaea alba* selected in this invention exhibits a synergistic effect in inhibiting the release of inflammatory factors, providing experimental evidence for its potential as a raw material for skin repair.

[0070] Example 3 Example 3 pertains to the preparation and testing of the compositions. The preparation process of the ginger root extract is as follows: Ginger (Zingiber officinale) root was mixed with 15 times its weight of a 75wt% ethanol solution, stirred and extracted at 60°C for 2 hours, centrifuged at 3000 rpm for 25 minutes, and the supernatant was concentrated under reduced pressure to remove the ethanol. The extract was then freeze-dried to a water content of 3.52wt%. The Yunnan Paris extract and white water lily extract were prepared in Example 2.

[0071] Composition a: Raw material composition, by weight percentage: 0.1% Sodium DNA (cosmetic grade PDRN, product model: RJMPDRN-C02, INCI name: Sodium DNA, Ruijiming (Shandong) Biotechnology Co., Ltd., hereinafter the same), 0.8% Hydrolyzed Sodium Hyaluronate (Nano HA® Enzyme-Cleaned Oligomeric Sodium Hyaluronate, hereinafter the same), 1.5% α-Glucan Oligosaccharide (trade name FLORASKIN L, Xianting (Guangzhou) Trading Co., Ltd., hereinafter the same), 0.15% Ectoin, 3% Plant-derived Ingredients, 15% Polyols, and the balance water to 100%. The plant-derived ingredients are Yunnan Paris extract, mulberry root extract 1-a, ginger root extract, and white water lily extract in a weight ratio of 1:1:0.3:0.9. The polyols are glycerol and 1,3-butanediol in a weight ratio of 1:3.

[0072] Preparation process of composition a: Add polyol and water to the reaction vessel, stir at 100 rpm for 5 minutes at room temperature, add sodium DNA, hydrolyzed sodium hyaluronate, α-glucan oligosaccharide, ectoine and plant-derived components, continue stirring for 25 minutes, sterilize, and fill to obtain the product.

[0073] Composition b: Raw material composition, by weight percentage: 0.12% sodium DNA, 0.85% hydrolyzed sodium hyaluronate, 1% α-glucan oligosaccharide, 0.12% ectoine, 2.9% plant-derived components, 18% polyols, and balance water to 100%. The plant-derived components are Yunnan Paris extract, mulberry root extract 1-b, ginger root extract, and white water lily extract in a weight ratio of 1:0.8:0.4:1.2. The polyols are glycerol, 1,3-propanediol, and 1,3-butanediol in a weight ratio of 1:1:4.

[0074] Preparation process of composition b: Add polyol and water to the reaction vessel, stir at 120 rpm for 7 minutes at room temperature, add sodium DNA, hydrolyzed sodium hyaluronate, α-glucan oligosaccharide, ectoine and plant-derived components, continue stirring for 20 minutes, sterilize, and fill to obtain the product.

[0075] Composition c: Raw material composition, by weight percentage: 0.8% hydrolyzed sodium hyaluronate, 1.5% α-glucan oligosaccharide, 0.15% ectoine, 0.5% plant-derived ingredients, 15% polyols, and balance water to 100%. The plant-derived ingredients are Yunnan Paris extract, mulberry root extract 1-a, ginger root extract, and white water lily extract in a weight ratio of 1:1:0.3:0.9. The polyols are glycerol and 1,3-butanediol in a weight ratio of 1:3.

[0076] Preparation process of composition c: Add polyol and water to the reaction vessel, stir at 100 rpm for 5 minutes at room temperature, add hydrolyzed sodium hyaluronate, α-glucan oligosaccharide, ectoine and plant-derived components, continue stirring for 25 minutes, sterilize, and fill to obtain the product.

[0077] The tyrosinase inhibition rate, TNF-α content, and inhibition rate of compositions a, b, and c were tested according to the test methods of Examples 1 and 2, and the results are shown in Tables 3 and 4. In the whitening effect test, compositions a to c were diluted with disodium hydrogen phosphate-citric acid buffer to obtain test solutions (composition concentration 35 mg / mL), with other parameters remaining unchanged. In the skin repair effect test (TNF-α content and inhibition rate), the test groups (corresponding to compositions a to c) were supplemented with fresh culture medium containing 0.5 μg / mL LPS and 22.5 mg / mL of compositions a to c, with other parameters remaining unchanged.

[0078] Table 3: Tyrosinase inhibition rates of compositions a, b, and c

[0079] Table 4: TNF-α content and inhibition rate of compositions a, b, and c

[0080] In Table 4: Compared with the model group: "*": p < 0.01; "**": p < 0.01; "***": p < 0.001.

[0081] Tables 3-4 examined the tyrosinase inhibition rate, TNF-α content, and inhibition rate of compositions a, b, and c to evaluate their whitening and skin-repairing effects. The results showed that composition a had the best and highest effect, followed by composition b, and composition c had the lowest. The activities of compositions a and b were similar, but both were significantly higher than composition c. Compared to composition a, composition c lacked 0.1% sodium DNate and had a lower content of plant-derived ingredients, resulting in a sharp drop in the tyrosinase inhibition rate from 75.92% to 41.67%. The TNF-α content of composition a was 979.75 pg / mL, with an inhibition rate of 26.19%, while the TNF-α content of composition c was 1187.43 pg / mL, with an inhibition rate of only 10.55%. The tests in Tables 3 and 4 show that the simultaneous use of sodium DNate and plant-derived ingredients in the compositions of this invention can significantly enhance the whitening and skin-repairing effects of the compositions. These compositions show good promise for use as cosmetic raw materials in cosmetics.

[0082] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A skin-repairing and skin-toning composition containing sodium DNA, characterized in that, Made from the following ingredients: sodium DNA, hydrolyzed sodium hyaluronate, α-glucan oligosaccharide, ectoine, plant-derived ingredients, polyols and water; The plant-derived ingredients include Yunnan Paris extract and white water lily extract in a weight ratio of 1:(0.5-1.5), as well as mulberry root extract; The preparation process of mulberry root extract includes: taking young mulberry roots, processing them and inoculating them into a culture medium to obtain callus tissue, culturing the callus tissue to produce adventitious roots, transferring the adventitious roots to a liquid culture medium containing L-phenylalanine and L-tyrosine for further culture, adding 85-95wt% ethanol to the adventitious roots obtained from further culture, heating and extracting, centrifuging, concentrating and drying the centrifuged supernatant to obtain the extract.

2. The skin repair and skin-evening composition containing sodium DNA according to claim 1, characterized in that, Polyols include at least one of ethylene glycol, propylene glycol, butanediol, glycerol, polyethylene glycol, and pentanediol.

3. The skin repair and skin-evening composition containing sodium DNA according to claim 1, characterized in that, It is made from the following raw materials by weight percentage: 0.001-0.5% sodium DNA, 0.01-1.5% hydrolyzed sodium hyaluronate, 1-3% α-glucan oligosaccharide, 0.001-0.25% ectoine, 2-6% plant-derived ingredients, 5-25% polyols and the balance water.

4. The skin repair and skin-evening composition containing sodium DNA according to claim 1, characterized in that, The dosage of sodium DNA is 0.005-0.2%.

5. The skin repair and skin-evening composition containing sodium DNA according to claim 1, characterized in that, The dosage of hydrolyzed sodium hyaluronate is 0.02-1%.

6. The skin repair and skin-evening composition containing sodium DNA according to claim 1, characterized in that, The amount of α-glucan oligosaccharide used is 1.2-2.5%.

7. The skin repair and skin-evening composition containing sodium DNA according to claim 1, characterized in that, The plant-derived components are in the following weight ratios: (0.8-1.2): (0.2-0.7): (0.5-1.5) extracts of Paris polyphylla, mulberry root, ginger root, and white water lily; and / or, the extracts of Paris polyphylla, ginger root, and white water lily are obtained by extraction with 65-85 wt% ethanol.

8. The skin repair and skin-evening composition containing sodium DNA according to claim 4, characterized in that, In the preparation process of mulberry root extract, the weight ratio of L-phenylalanine to L-tyrosine is 1:1-2.

9. A preparation process for a skin repair and skin-evening composition containing sodium DNA according to any one of claims 1-8, characterized in that, This includes the step of mixing the raw materials.

10. The use of a skin repair and skin-evening composition containing sodium DNA according to any one of claims 1-8 in the preparation of moisturizing, soothing, and whitening cosmetics.

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