Insecticidal composition and use thereof

Abstract

The present application relates to the field of pesticide insecticide, and particularly to a kind of insecticide composition and its application.The composition includes active ingredient, and the active ingredient includes zaname and spirotetramat, and the mass ratio of zaname and spirotetramat is 2:1~1:3.The composition has good synergistic effect on piercing-sucking pests such as whitefly, resistant cotton aphid, etc., and has excellent quick-acting and persistence, can achieve better control effect than single agent at lower dose, can effectively delay the development of insect resistance, has long-lasting efficacy, wide control spectrum, good safety, environmental protection, low toxicity and sustainability.

Description

Technical Field

[0001] This invention relates to the field of pesticides and insecticides, and in particular to an insecticide composition containing pyraclostrobin and spirotetramat and its application. Background Technology

[0002] whiteflies Bemisia tabaci *Gennadius*, belonging to the family Aleyrodidae in the order Hemiptera, is a global agricultural pest, considered a "super pest" by the academic community. This insect has evolved rapidly, with different biotypes including A, B, and Q. It has a high reproductive capacity and hosts over 600 plant species. It not only causes sooty mold by sucking plant sap and secreting honeydew, but also transmits plant viruses, thus harming crops. Aphids are also important global pests, belonging to the superfamily Aphidoidea in the order Hemiptera. There are many species, with approximately 100 species capable of causing economic losses, including the peach aphid. Myzus persica and cotton aphids Aphis gossypii Ranked first among the world's top ten pests, aphids have a short life cycle, rapid reproduction rate, parthenogenesis, a wide host range, and are also the most important vector of plant viruses, posing a huge threat to my country's agricultural and forestry production. Both of these pests have significant generational overlap, requiring continuous pesticide application, thus leading to varying degrees of resistance to organophosphates, neonicotinoids, and other insecticides.

[0003] Dimpropyridaz, developed by BASF in 2009, is a novel insecticide containing pyrazine and pyrazole amide fragments. It exhibits good stomach poison and contact activity against pests such as whiteflies, aphids, thrips, mealybugs, psyllids, and leafhoppers, as well as good systemic activity (primarily through upward movement of the xylem). In 2023, the International Resistant Pesticides Action Committee (IRAC) classified it as a new Group 36 (stringer modulators), making it the first piercing-sucking insecticide to receive this new mode of action classification since 2015. However, its specific target differs from other stringer insecticides (such as pymetrozine, tebufenozide, and flonicamid). It is hydrolyzed or enzymatically converted into a pyrpropyridazine metabolite that acts on the upstream site of the TRPV channel, acting as a stringer channel inhibitor. Its novel target makes it a promising new tool for pest resistance management. Spirotetramat is a cyclic ketone enol insecticide and acaricide with high activity against piercing-sucking pests such as whiteflies and aphids. An important characteristic is its bidirectional translocation between the xylem and phloem of plants. Once absorbed by the plant, it is rapidly hydrolyzed and converted into spirotetramat-enol. This substance acts on the insect's acetyl-CoA carboxylase (ACC), blocking the lipid synthesis pathway and ultimately interfering with the development of eggs and nymphs and the reproduction of adults, thus achieving its insecticidal effect. While tebuconazole and spirotetramat have different mechanisms of action against pests, exhibiting significant synergistic effects and effectively delaying the development of pesticide resistance, there are currently no reports of their combined use. Summary of the Invention

[0004] To address the challenge of controlling resistant whiteflies, aphids, and other insects with piercing-sucking mouthparts, this invention provides an insecticidal composition comprising active ingredients, including chlorfenapyr and spirotetramat, wherein the mass ratio of chlorfenapyr to spirotetramat is 2:1 to 1:3.

[0005] The composition of this invention has a good synergistic effect on piercing-sucking pests such as whiteflies and resistant cotton aphids. It has excellent rapid and sustained effects, and can achieve better control effects than single agents at lower doses. It can effectively delay the development of pesticide resistance in pests, has a long-lasting effect, a broad spectrum of control, good safety, and is environmentally friendly, low in toxicity, and sustainable.

[0006] Furthermore, the mass ratio of acetamiprid to spirotetramat is 1:1 to 1:2.

[0007] Furthermore, the active ingredient constitutes 1% to 80% of the total mass percentage of the insecticidal composition; Preferably, the active ingredient accounts for 10% to 60% of the total mass percentage of the insecticidal composition.

[0008] Furthermore, the insecticidal composition also includes agriculturally acceptable auxiliary ingredients; Preferably, the auxiliary components are selected from one or more of dispersants, emulsifiers, disintegrants, wetting agents, antifreeze agents, preservatives, defoamers, thickeners, solvents, pH adjusters, and fillers.

[0009] Furthermore, the composition is prepared into an agriculturally permissible dosage form, which is selected from solid dosage forms and / or liquid dosage forms.

[0010] Furthermore, the liquid formulation is selected from at least one of suspending agents, dispersible oil suspensions, and soluble agents; the solid formulation is selected from water-dispersible granules.

[0011] The present invention also provides the application of the insecticidal composition in the control of piercing-sucking pests.

[0012] Furthermore, the piercing-sucking mouthpart pest is selected from at least one of the following: whitefly, whitefly, and aphid.

[0013] Furthermore, the insecticidal composition is applied in an effective dose to the piercing-sucking pests that need to be controlled or to the medium in which the piercing-sucking pests grow.

[0014] Beneficial effects: (1) The active ingredients of the composition described in this invention, tebuconazole and spirotetramat, have different mechanisms of action. The combination of the two can achieve complementary advantages: the growth-regulating activity of spirotetramat can make up for the lack of ovicidal ability of tebuconazole and can also achieve synergistic effect by inhibiting nymph development and adult reproduction. On the other hand, tebuconazole has a unique mechanism of action, high biological activity, and is effective against adult piercing-sucking pests with high safety. At the same time, the two components have no overlapping action sites and have strong systemic properties. After being combined, they act on different insect stages and have a good synergistic effect, which improves the speed and duration of action, and has a good insecticidal effect. It can achieve better control effect than single agent at a lower dose.

[0015] (2) The composition of the present invention can effectively delay the development of drug resistance in piercing-sucking pests, has a long-lasting effect, a broad spectrum of control, good safety, and is environmentally friendly, low in toxicity, and sustainable. Detailed Implementation

[0016] To make the technical solution and beneficial effects of the present invention more apparent and understandable, a detailed description is provided below by listing specific embodiments. Unless otherwise defined, the technical and scientific terms used herein have the same meanings as those in the technical field to which this application pertains.

[0017] In this invention, the term "and / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, or B existing alone. A and B can be singular or plural.

[0018] In this invention, "at least one" means one or more, and "more than one" means two or more. "At least one of the following" or similar expressions refer to any combination of these items, including any combination of a single item or a plurality of items. For example, "at least one of a, b or c", or "at least one of a, b and c", can both mean: a, b, c, ab (i.e., a and b), ac, bc, or abc, where a, b, and c can be a single item or multiple items.

[0019] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the present invention, the two endpoints of each numerical range and any value between the two endpoints may be selected.

[0020] In this specification, references to "some specific / preferred embodiments," "other specific / preferred embodiments," "implementation," "implementation," etc., refer to specific elements (e.g., features, structures, properties, and / or characteristics) related to that embodiment / implementation, which are included in at least one of the embodiments described herein and may or may not be present in other embodiments. Furthermore, it should be understood that these elements may be combined in any suitable manner in various embodiments.

[0021] This invention provides an insecticidal composition comprising an active ingredient, wherein the active ingredient comprises chlorfenapyr and spirotetramat, and the mass ratio of chlorfenapyr to spirotetramat is 2:1 to 1:3, such as 2:1, 1:1, 1:2, 1:3, etc. Other specific values ​​within the above range are also acceptable and will not be listed here.

[0022] In some embodiments, the mass ratio of tebuconazole to spirotetramat is 1:1 to 1:2, such as 1:1, 1:2, 2:3, etc. Other specific values ​​within the above range are also acceptable and will not be listed here.

[0023] In some embodiments, the active ingredient constitutes 1% to 80% of the total mass percentage of the insecticidal composition, such as 1%, 20%, 40%, 60%, 80%, etc. Other specific values ​​within the above range are also acceptable and will not be listed here.

[0024] In some embodiments, the active ingredient constitutes 10% to 60% of the total mass percentage of the insecticidal composition, such as 10%, 20%, 40%, 60%, etc. Other specific values ​​within the above range are also acceptable and will not be listed here.

[0025] In some embodiments, the insecticidal composition further includes agriculturally acceptable auxiliary ingredients.

[0026] In some embodiments, the auxiliary ingredients are selected from one or more of dispersants, emulsifiers, disintegrants, wetting agents, antifreeze agents, preservatives, defoamers, thickeners, solvents, pH adjusters, and fillers.

[0027] In some embodiments, the dispersant is selected from one or more of tristyrylphenol polyoxyethylene ether phosphate, polycarboxylate, sodium naphthalenesulfonic acid formaldehyde condensate, and lignin sulfonate.

[0028] In some embodiments, the emulsifier is selected from one or more of EO-PO block copolymers and castor oil polyoxyethylene ether.

[0029] In some embodiments, the disintegrant is selected from one or more of urea, ammonium sulfate, sodium chloride, and sodium carboxymethyl starch.

[0030] In some embodiments, the wetting agent is selected from one or more of sodium dodecyl sulfate, calcium dodecylbenzene sulfonate, fatty alcohol polyoxyethylene ether, alkyl glycoside, and sodium fatty alcohol polyoxyethylene ether sulfonate.

[0031] In some embodiments, the antifreeze is selected from one or more of propylene glycol, glycerol, and polyethylene glycol.

[0032] In some embodiments, the preservative is selected from one or more of sodium benzoate and potassium sorbate.

[0033] In some embodiments, the defoamer is selected from one or more of silicone defoamers, polyether defoamers, and polyether-modified silicone defoamers.

[0034] In some embodiments, the thickener is selected from one or more of organobentonite, silica, xanthan gum, magnesium aluminum silicate, polyvinyl alcohol, sodium carboxymethyl cellulose, and sodium polyacrylate.

[0035] In some embodiments, the solvent is selected from one or more of aqueous solvents, oil-based solvents, etc.

[0036] In some embodiments, the aqueous solvent is selected from water.

[0037] In some embodiments, the oily solvent is selected from one or more of turpentine, N-methylpyrrolidone, and methyl oleate.

[0038] In some embodiments, the pH adjuster is selected from one or more of benzoic acid, citric acid, sodium bicarbonate, and triethanolamine.

[0039] In some embodiments, the filler is selected from one or more of kaolin, diatomaceous earth, attapulgite, starch, and light calcium carbonate.

[0040] In some embodiments, the insecticidal composition is prepared into an agriculturally permissible formulation, selected from liquid and / or solid formulations.

[0041] In some embodiments, the liquid formulation is selected from at least one of suspending agents, dispersible oil suspensions, and soluble agents.

[0042] In some embodiments, the solid formulation is selected from water-dispersible granules.

[0043] The present invention also provides the application of the above-mentioned insecticidal composition in the control of piercing-sucking agricultural and forestry pests.

[0044] In some embodiments, the pest is selected from piercing-sucking insects.

[0045] In some embodiments, the sucking pest is selected from one or more of the following: whitefly, whitefly, and aphid.

[0046] In some embodiments, the insecticidal composition is applied in an effective dose to piercing-sucking pests or the medium in which they grow, which require control.

[0047] The method of the present invention will be described below through specific embodiments. It should be understood that these embodiments are used to illustrate the basic principles, main features and advantages of the present invention, and the present invention is not limited to the scope of the following embodiments. The implementation conditions used in the embodiments can be further adjusted according to specific requirements, and the implementation conditions not specified are usually the conditions in conventional experiments.

[0048] Example 1 This embodiment provides a 60% pyraclostrobin•spirotetramat water-dispersible granule, which is prepared from the following components: 40% pyraclostrobin Spirotetramethrin 20% Naphthalenesulfonate formaldehyde condensate (NNO) 5.0% Polycarboxylate (SP-2700) 3.0% Sodium dodecyl sulfate (K12) 1.5% Urea 5.0% Ammonium sulfate 3.0% Organosilicon defoamer (DF-6800) 0.3% Diatomaceous earth 6.0% Polyvinyl alcohol (PVA 1788) 0.5% Kaolin to make up 100% Preparation method: Immunochlorfon, spirotetramat technical grade, kaolin, diatomaceous earth, naphthalenesulfonate formaldehyde condensate (NNO), polycarboxylate (SP-2700), K12, urea, ammonium sulfate, and organosilicon defoamer (DF-6800) are added to a mixer in a specific ratio and thoroughly mixed to obtain a mixture. The mixture is then pulverized using an air jet mill to a D90 ≤ 10 μm. The pulverized granules are added to an appropriate amount of hot water along with polyvinyl alcohol (PVA 1788), and then placed in a mixer and stirred evenly. The mixture is then granulated using an extrusion granulator with a 0.8~1.0 mm aperture. The wet granules are dried in a fluidized bed at 60~65℃ until the moisture content is ≤2%. The target particle size particles are collected by sieving, and those that pass the test are the 60% immunochlorfon•spirotetramat water-dispersible granules.

[0049] Example 2 This embodiment provides a 20% pyraclostrobin•spirotetramat dispersible oil suspension, which is prepared from the following components: 10% pyraclostrobin Spirotetramethrin 10% Calcium dodecylbenzenesulfonate 4.0% Castor oil polyoxyethylene ether (EL-40) 6.0% EO-PO block copolymer 2.0% Organosilicon defoamer (SXP-820) 0.3% Organic bentonite 0.6% 0.2% fumed silica (gas phase method) Methyl oleate replenished to 100%. Preparation method: Most of the methyl oleate was mixed with organobentonite and fumed silica and heated and sheared until gelled. After cooling, calcium dodecylbenzenesulfonate, castor oil polyoxyethylene ether and EO-PO block copolymer were added and stirred evenly. SXP-820 defoamer was added, and then acetamiprid and spirotetramat technical were added and sheared at high speed to obtain a coarse slurry. The coarse slurry was horizontally milled with 0.6-0.8 mm zirconia beads (temperature controlled ≤30 ℃) until the particle size D90≤4 μm. After completion, the remaining methyl oleate was added, and the mixture was degassed under vacuum, filtered, and tested to obtain 20% acetamiprid•spirotetramat dispersible oil suspension.

[0050] Example 3 This embodiment provides a 30% pyraclostrobin•spirotetramat soluble concentrate, which is prepared from the following components: 10% pyraclostrobin Spirotetramethrin 20% 12% turpentine N-methylpyrrolidone 15% EO-PO block copolymer 3.0% Fatty alcohol polyoxyethylene ether 2.0% Calcium dodecylbenzenesulfonate 2.0% Organosilicon defoamer (SXP-820) 0.2% Replenish water to 100%. Preparation method: Turpentine oil and N-methylpyrrolidone are added to a reaction vessel, and tebuconazole and spirotetramat technical materials are added under stirring. The mixture is heated to 40-50 °C and stirred continuously until the technical materials are completely dissolved. Then, EO-PO block copolymer, fatty alcohol polyoxyethylene ether and calcium dodecylbenzenesulfonate are added in sequence. After stirring evenly, the mixture is stirred at high speed and the aqueous phase is slowly added dropwise until the total volume is reached. The phase is inverted to form a transparent and homogeneous solution. Then, SXP-820 defoamer is added. After vacuum degassing and precision filtration, the 30% tebuconazole•spirotetramat soluble concentrate is obtained after passing the test.

[0051] Example 4 This embodiment provides a 40% pyraclostrobin•spirotetramat suspension, which is prepared from the following components: 10% pyraclostrobin Spirotetramethrin 30% Tristyrene-phenol polyoxyethylene ether phosphate 5.0% Polycarboxylate (SP-2700) 2.5% Fatty alcohol polyoxyethylene ether 1.5% Propylene glycol 5.0% Magnesium aluminum silicate 0.6% Xanthan gum 0.15% Sodium benzoate 0.20% Citric acid 0.10% Organosilicon defoamer (SXP-820) 0.3% Replenish deionized water to 100%. Preparation method: Most of the deionized water, propylene glycol, citric acid, and sodium benzoate were added to a reaction vessel and stirred to dissolve. Magnesium aluminum silicate was slowly added under high-speed shearing and stirred until fully hydrated. Xanthan gum was then added and shearing continued until a homogeneous, transparent gel was obtained. Tristyrene-based phenolic polyoxyethylene ether phosphate, polycarboxylate (SP-2700), and fatty alcohol polyoxyethylene ether were added to the gel system sequentially and stirred until homogeneous. SXP-820 defoamer was added, followed by chlorfenapyr and spirotetramat technical, and dispersed under high-speed shearing to obtain a coarse slurry. The coarse slurry was transferred to a horizontal sand mill and ground with 0.8–1.0 mm zirconia beads until D90 ≤ 3μm (grinding temperature strictly controlled ≤35℃ to prevent spirotetramat melting). After grinding, the remaining deionized water was added to adjust the viscosity, air bubbles were removed under vacuum, and the mixture was filtered. Once the test was passed, a 40% chlorfenapyr•spirotetramat suspension was obtained.

[0052] Test Example 1: Indoor Combined Toxicity Determination Experiment of Immunosorbide and Spirotetramethrin against Whiteflies Test insect: whitefly Bemisia tabaci The insects were collected from a cantaloupe greenhouse in Qiuyu Village, Foluo Town, Ledong Li Autonomous County, Hainan Province. The collected insects were healthy and active adults.

[0053] Test reagents: pyraclostrobin and spirotetramat.

[0054] Experimental method: Spraying method. Accurately weigh the technical grade of the single agent and mixture, dissolve the sample in acetone, and then dilute with 0.5% Tween-80 aqueous solution to prepare the corresponding mass concentration gradients. Five concentration gradients were set up for each agent treatment. Each insect rearing cage (120 mesh screen, with a single-sleeve door on one side of the front, 35cm in size) was used. 3 Place a cucumber seedling with 4-5 leaves in a cage. Introduce collected adult whiteflies into the cage. After the insects have adapted to the environment, count the number of live insects on both sides of all leaves in each cage. Then, spray the cage with an electric sprayer (1L, Deli brand) containing the prescribed concentration of pesticide. Repeat each treatment 4 times. After treatment, place the cage in a culture room (temperature 25±1℃, relative humidity 70%). Criteria for determining the life or death of adult whiteflies: If the whitefly is flying normally, it is considered alive; if the insect is unable to move or its wings are forked or deformed, it is considered dead.

[0055] Data Processing: The number of live whiteflies in each treatment was investigated 24 hours after application. The mortality rate was determined by comparing the number of live whiteflies with the number before application. Regression analysis was performed on the logarithmic values ​​of each pesticide concentration and the corresponding mortality probability values ​​to calculate the LC50 of each pesticide. 50 And the co-toxicity coefficient (CTC) of the mixture was calculated according to Sun Yunpei's method.

[0056] Actual Toxicity Index (ATI) = (LC50 of Standard Reagent) 50 / LC of the test reagent 50 )×100.

[0057] Theoretical toxicity index (TTI) = (toxicity index of A × percentage of A in the mixture) + (toxicity index of B × percentage of B in the mixture).

[0058] Co-toxicity coefficient (CTC) = [Actual toxicity index (ATI) of mixture / Theoretical toxicity index (TTI) of mixture] × 100.

[0059] A mixture with a CTC ≥ 120 exhibits a synergistic effect; a CTC ≤ 80 exhibits an antagonistic effect; and a CTC < 120 exhibits an additive effect.

[0060] Table 1. Toxicity test results of different ratios of chlorfenapyr and spirotetramat against whiteflies.

[0061] Table 2. Combined toxicity effects of different ratios of chlorfenapyr and spirotetramat on whiteflies.

[0062] Table 1 shows the LC50 of chlorfenapyr and spirotetramat against whiteflies. 50 The concentrations were 2.32 mg / L and 4.17 mg / L, respectively. Table 2 shows that the combined toxicity of chlorfenapyr and spirotetramat against whiteflies at different ratios showed that the ratios of 2:1, 1:1, 1:2, and 1:3 exhibited a synergistic effect (CTC > 120). In particular, the ratios of 1:2 and 1:1 showed the highest insecticidal activity against whiteflies, with co-toxicity coefficients reaching 167.23 and 154.47, respectively, indicating the best synergistic effect.

[0063] Test Example 2: Indoor Combined Toxicity Determination Experiment of Immunosorbide and Spirotetramethrin against Cotton Aphids Test insect: cotton aphid Aphis gossypii The insects were collected from cotton fields in Gaogedaquan Village, Kaiganqi Township, Kuitun City, Xinjiang Uygur Autonomous Region, and adult insects of uniform size were selected.

[0064] Test reagents: pyraclostrobin and spirotetramat.

[0065] Experimental Method: Spraying method. Accurately weigh the technical grade of single-agent and mixed formulations, dissolve the samples in acetone, and then dilute with 0.5% Tween-80 aqueous solution to prepare corresponding mass concentration gradients. Five concentration gradients were set up for each agent treatment. Petri dishes were used as the experimental apparatus. Filter paper was placed at the bottom of the dish, and absorbent cotton balls were placed on the sides for moisture retention. One relatively flat leaf was placed in each dish. The cotton leaves were placed on the rotating platform of the bioassay spray tower. The pesticide tank was cleaned beforehand and the corresponding treatment solution was added. The switch was turned on for quantitative and uniform spraying, aiming to moisten both sides of the leaf without dripping water. Each treatment was repeated 4 times, with one water control group included. After spraying, the leaves were allowed to air dry naturally before being placed in petri dishes. Cotton aphids collected and reared beforehand were transferred to the cotton leaves using a No. 0 brush, 100 aphids per leaf. Finally, the leaves were placed in an artificial climate incubator (temperature 25±1℃, relative humidity 75±5%). Criteria for determining whether cotton aphids are alive or dead: Gently touch the insect's body with a paintbrush. If the insect moves normally, it is considered alive. If the insect does not move or cannot crawl normally, and its body surface is dull, it is considered dead. The test is based on counting the number of live aphids on the leaves.

[0066] Data Processing: The number of live cotton aphids in each treatment was investigated 24 hours after application. The mortality rate was determined by comparing the number of live aphids with the number before application. Regression analysis was performed on the logarithmic values ​​of each pesticide concentration and the corresponding mortality probability values ​​to calculate the LC50 of each pesticide. 50 And the co-toxicity coefficient (CTC) of the mixture was calculated according to Sun Yunpei's method.

[0067] Actual Toxicity Index (ATI) = (LC50 of Standard Reagent) 50 / LC of the test reagent 50 )×100.

[0068] Theoretical toxicity index (TTI) = (toxicity index of A × percentage of A in the mixture) + (toxicity index of B × percentage of B in the mixture).

[0069] Co-toxicity coefficient (CTC) = [Actual toxicity index (ATI) of mixture / Theoretical toxicity index (TTI) of mixture] × 100.

[0070] A mixture with a CTC ≥ 120 exhibits a synergistic effect; a CTC ≤ 80 exhibits an antagonistic effect; and a CTC < 120 exhibits an additive effect.

[0071] Table 3. Toxicity determination of different ratios of acetamiprid and spirotetramat against cotton aphids.

[0072] Table 4. Combined toxicity effects of different ratios of acetamiprid and spirotetramat against cotton aphids.

[0073] Table 3 shows the LC50 of pyraclostrobin and spirotetramat against the cotton aphid in Xinjiang. 50 The concentrations were 1.73 mg / L and 3.86 mg / L, respectively. Table 4 shows that the combined toxicity of different ratios of chlorfenapyr and spirotetramat against cotton aphids was synergistic when the ratios were 3:1, 2:1, 1:1, 1:2, and 1:3 (CTC > 120). In particular, the ratios of 1:2 and 1:1 showed the highest insecticidal activity against cotton aphids, with co-toxicity coefficients reaching 184.92 and 173.13, respectively, indicating the best synergistic effect.

[0074] Test Example 3: Field efficacy trial of a combination of chlorfenapyr and spirotetramat for controlling whiteflies. The experiment was conducted in a cantaloupe greenhouse in Fengting Village, Banqiao Town, Dongfang City, Hainan Province, in November 2024, using a spraying method. Seven treatments were included, with water as a blank control and tebuconazole and spirotetramat as control agents. Four different ratios of tebuconazole and spirotetramat were used (see Examples 1-4), with each treatment repeated four times. Each treatment was diluted to the corresponding dilution factor according to the dosages in Table 5. A 5mL syringe was used to draw the appropriate amount of agent, which was added to a measured amount of water and stirred well before use. For example, 2.5mL of 120g / L tebuconazole formulation was dissolved in an appropriate amount of water, then water was added to a final volume of 15L and mixed thoroughly before use. Whiteflies prefer windy areas and the undersides of leaves, and are typically more prevalent at the beginning and end of plants in greenhouses. Before applying pesticides, seven rows of cantaloupe seedlings were selected as a test treatment. Labels were attached to the petioles of the cantaloupe leaves with adhesive paper, and the leaves were gently turned over to survey and record the initial number of live whiteflies (the experimental stage was adults). The prepared pesticide solution was poured into a 20L backpack electric sprayer, and the sprayer was used to apply the pesticide to the cantaloupe plants of each treatment, using 30L of water per acre. Seven days after application, the number of live whiteflies on the labeled leaves of all treatments was surveyed to calculate the control effect.

[0075] In the development of pesticide compound formulations, the Colby method, proposed by SRColby in 1967, is a classic statistical method used to evaluate the type of synergistic effect (determining whether the mixture is synergistic, additive, or antagonistic). It is widely used in evaluating the combined effects of herbicides, insecticides, and fungicides. Its calculation formula is E0 = Ax + By - (Ax) / (By) (By / 100) , A and B represent the effects of active ingredient A (concentration x mg / L) and active ingredient B (dosage y mg / L) on the target when used alone, respectively. E0 is the expected effect after mixing active ingredients A and B. If the actual field effect E > E0, there is a synergistic effect; if E ≈ E0, there is an additive effect; if E < E0, there is an antagonistic effect.

[0076] Calculation formula: Control effect = [1 - (number of insects in the control area before treatment × number of insects in the treatment area after treatment) / (number of insects in the control area after treatment × number of insects in the treatment area before treatment)] × 100.

[0077] Table 5. Test results of pymetrozine, spirotetramat, and their combinations in controlling whiteflies.

[0078] Table 5 shows the experimental results. When applying chlorpyrifos, spirotetramat, and different combinations (Examples 1-4) of these agents, the efficacy was assessed 7 days after application. With the same dosage, the field control efficacy of the chlorpyrifos-spirotetramat mixtures in different ratios was significantly higher than the expected efficacy calculated by the Colby method. This indicates that the combination of the two agents has a significant synergistic effect and a long-lasting effect. None of the treatments had any adverse effects on cantaloupe or other organisms, demonstrating good safety.

[0079] Test Example 4: Field efficacy trial of fenpropathrin-spirotetramat combination for controlling cotton aphids in Xinjiang The experiment was conducted in cotton fields in Gaogedaquan Village, Kaiganqi Township, Kuitun City, Xinjiang Uygur Autonomous Region, in June 2025, using a spraying method. Seven treatments were included, with a water treatment as a blank control and tebuconazole and spirotetramat as control agents. Four different ratios of tebuconazole·spirotetramat were used (see Examples 1-4), prepared according to the dosages in Table 6, using the same preparation method as in Test Example 3. Before application, experimental plots of 100㎡ were divided, with five points marked on cotton stems or leaves to count the initial live insect population (without distinguishing between developmental stages). A 20L backpack electric sprayer carrying the corresponding pesticide was used to apply the pesticide to each plot, using 30L of water per acre. Seven days after application, the number of live insects in all treatments was investigated, and the control effect was calculated.

[0080] Calculation formula: Control effect = [1 - (number of insects in the control area before treatment × number of insects in the treatment area after treatment) / (number of insects in the control area after treatment × number of insects in the treatment area before treatment)] × 100.

[0081] Table 6. Test results of pymetrozine, spirotetramat, and their combinations in controlling cotton aphids.

[0082] Table 6 shows the experimental results. When applying acetamiprid, spirotetramat, and different combinations (Examples 1-4) to cotton aphids, the efficacy was assessed 7 days after application. Under the same experimental dosage, the four acetamiprid agents... Spirotetracycline mixtures showed significantly better field control efficacy than the expected efficacy (E0), with a very significant synergistic effect and outstanding sustained efficacy. During the trial, none of the treatments had any adverse effects on cotton growth, and the treatments were safe for non-target organisms in the field.

[0083] It should be understood that the above embodiments are exemplary and are not intended to encompass all possible implementations included in the claims. Various modifications and changes can be made to the above embodiments without departing from the scope of the invention. Similarly, the various technical features of the above embodiments can be arbitrarily combined to form other embodiments of the invention that may not be explicitly described. Therefore, the above embodiments only illustrate several implementations of the invention and do not limit the scope of protection of this patent.

Claims

1. An insecticidal composition, characterized in that, The composition includes active ingredients, which include fenpropathrin and spirotetramat, wherein the mass ratio of fenpropathrin to spirotetramat is 2:1 to 1:

3.

2. The insecticidal composition according to claim 1, characterized in that, The mass ratio of acetamiprid to spirotetramat is 1:1 to 1:

2.

3. The insecticidal composition according to claim 1 or 2, characterized in that, The active ingredient constitutes 1% to 80% of the total mass percentage of the insecticidal composition; Preferably, the active ingredient accounts for 10% to 60% of the total mass percentage of the insecticidal composition.

4. The insecticidal composition according to any one of claims 1-3, characterized in that, The composition further includes auxiliary components selected from one or more of dispersants, emulsifiers, disintegrants, wetting agents, antifreeze agents, preservatives, defoamers, thickeners, solvents, pH adjusters, and fillers.

5. The insecticidal composition according to any one of claims 1-4, characterized in that, The composition is prepared into an agriculturally permissible dosage form, which is selected from solid dosage forms and / or liquid dosage forms.

6. The insecticidal composition according to claim 5, characterized in that, The liquid formulation is selected from at least one of suspending agents, dispersible oil suspensions, and soluble agents; the solid formulation is selected from water-dispersible granules.

7. The use of the insecticidal composition according to any one of claims 1-6 in the control of piercing-sucking pests.

8. The application according to claim 7, characterized in that, The piercing-sucking mouthpart pests are selected from at least one of the following: whitefly, whitefly, and aphid.

9. The application according to claim 8, characterized in that, The insecticidal composition is applied in an effective dose to the piercing-sucking pests or the medium in which they grow, which require control.

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