A method for tracing flue-cured tobacco according to analysis of glandular hair secretion content of flue-cured tobacco
Patent Information
- Application Number
- CN202611199854.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-08-07
- Publication Date
- 2026-11-03
AI Technical Summary
Existing technologies fail to effectively utilize the differences in glandular trichome secretion content across different regions and leaf types of tobacco leaves, resulting in low tobacco leaf utilization and difficulty in improving cigarette product quality.
By dividing tobacco leaves into 14 regions and cutting them, the content of glandular trichome secretions in each region was detected. Cluster analysis was used to trace the origin of tobacco leaves of different types in different regions. The content of α-cephalitol and β-cephalitol was used to trace the origin of tobacco leaves.
It has enabled the refined utilization of tobacco leaves, improved the utilization rate of tobacco leaves and the quality of cigarette products, and provided technical support to enhance the quality of cigarette products.
Smart Images
Figure CN123029115A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of tobacco processing technology, and in particular relates to a method for tracing the origin of tobacco leaves based on the analysis of the content of secretions from the glandular trichomes of tobacco leaves. Background Technology
[0002] With the continuous upgrading of cigarette brand structure, the contradiction between the brand structure and the tobacco supply structure has become increasingly prominent, especially with the strong demand for high-quality cigarettes from certain specialty producing areas, particularly those from central and upper-grade regions. However, in the tobacco production stage, the proportion of high-quality tobacco has already reached about 70%, and further increases are no longer in line with the laws of agricultural development; the tobacco supply structure has reached its limit. Meanwhile, people's demands for cigarette quality are rising daily. Balancing the tobacco supply structure with people's quality requirements is a significant and challenging task facing cigarette manufacturers.
[0003] Cigarettes are essentially made from tobacco leaves, and the quality of tobacco leaves is mainly reflected in three aspects: sensory quality, intrinsic quality, and safety, ultimately manifesting in consumer satisfaction with cigarettes. The aroma of tobacco leaves is the core of their quality, and improving the aroma of flue-cured tobacco is key to enhancing its quality. Glandular trichomes, located on the epidermal cell protrusions of most vascular flue-cured tobacco leaves, not only protect the leaves from insect infestation but also prevent ultraviolet radiation and moisture loss. They are also the primary sites of aroma synthesis, significantly influencing the aroma and quality of the tobacco leaves. However, due to the numerous factors affecting tobacco leaf quality, even different areas of the same leaf can exhibit variations in the accumulation of various components after curing due to differences in light conditions and physiological characteristics. Currently, there are no reports on the distribution patterns of glandular trichome secretions in different areas of tobacco leaves, nor on using the analysis of glandular trichome secretion content to trace the origin of tobacco leaves, thereby improving the utilization rate of tobacco leaves and the quality of cigarette products. Based on this, this invention is proposed. Summary of the Invention
[0004] In view of this, the purpose of this invention is to provide a method for tracing the origin of tobacco leaves based on the analysis of glandular trichome secretion content. This invention obtains the glandular trichome secretion content in each segmented region by cutting and measuring the tobacco leaves. Cluster analysis is then used to cluster the glandular trichome secretion content in different types of tobacco leaves after threshing, tracing the location of different tobacco leaf types after threshing, thus providing technical support for improving the quality of cigarette products.
[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides a method for tracing the origin of tobacco leaves based on the analysis of secretions from the glandular trichomes, comprising the following steps: (1) Divide the tobacco leaves into 14 regions and further subdivide them according to their regions; (2) The content of glandular trichome secretions in tobacco leaves in each location was detected to obtain the quality characteristics data of tobacco leaves in different locations; (3) The tobacco leaves to be tested were blown and separated into different types of tobacco flakes. The content of secretions from the trichomes of different types of tobacco flakes was detected to obtain quality characteristic data of different types of tobacco flakes. (4) Perform cluster analysis on the quality characteristic data of tobacco leaves from different locations obtained in step (2) and the quality characteristic data of tobacco leaves of different types obtained in step (3) to obtain the source tracing results of tobacco leaves of different types in tobacco leaf locations; The content of the secretions from the cilia includes the content of α-cephalitol and β-cephalitol.
[0006] As a preferred method, the division of the location in step (1) is as follows: the tobacco leaf is divided into six locations along the leaf base to the leaf tip: leaf base, near leaf base, lower leaf middle, upper leaf middle, near leaf tip and leaf tip. Then, the four locations of near leaf base, lower leaf middle, upper leaf middle and near leaf tip are vertically divided into five parts to obtain 14 locations. The five parts are three regions: near the leaf margin, near the middle leaf, and near the midrib. The region near the midrib is the axis of symmetry, and the parts symmetrical to each other on both sides are the same region.
[0007] Preferably, the slicing is done at equal intervals.
[0008] Preferably, the tobacco leaves are sourced from Xuchang.
[0009] Preferably, the tobacco flakes are categorized into large flakes, medium flakes, small flakes, fragments, and dust, wherein large flakes are >25.4mm, medium flakes are <25.4mm, small flakes are 6.35mm <12.7mm, fragments are 2.36mm <6.35mm, and dust is ≤2.36mm.
[0010] This invention provides the application of the method in formulating refined leaf-pruning parameters.
[0011] The present invention also provides the application of the method in improving the utilization rate of tobacco leaves.
[0012] Compared with existing technologies, the present invention has the following beneficial effects: The present invention provides a method for tracing the origin of tobacco leaves based on the analysis of glandular trichome secretion content. The present invention obtains samples from 14 different locations by dividing and slicing tobacco leaves into regions. By detecting the glandular trichome secretion content in samples from different regions, the secretion content in different regions is obtained. Cluster analysis and other statistical data methods are used to perform cluster analysis on the glandular trichome secretion content in different types of tobacco leaves after harvesting, tracing the origin of different tobacco leaf regions after harvesting, and providing technical support for promoting the improvement of cigarette product quality. Attached Figure Description
[0013] Figure 1 A schematic diagram showing the division of the 14 regions; Figure 2 The results of determining the content of glandular trichome secretions in tobacco leaves from different locations and different types in the Xuchang production area; Figure 3 Cluster analysis results of glandular trichome secretion content in tobacco leaves from the Xuchang production area. Detailed Implementation
[0014] This invention provides a method for tracing the origin of tobacco leaves based on the analysis of secretions from the glandular trichomes, comprising the following steps: (1) Divide the tobacco leaves into 14 regions and further subdivide them according to their regions; (2) The content of tobacco gland secretions in each location was detected to obtain the quality characteristics data of tobacco leaves in different locations; (3) The tobacco leaves to be tested were blown and separated into different types of tobacco flakes. The content of glandular trichome secretions of different types of tobacco flakes was detected to obtain quality characteristic data of different types of tobacco flakes. (4) Perform cluster analysis on the quality characteristic data of tobacco leaves from different locations obtained in step (2) and the quality characteristic data of tobacco leaves of different types obtained in step (3) to obtain the source tracing results of tobacco leaves of different types in tobacco leaf locations; The content of the secretions from the cilia includes the content of α-cephalitol and β-cephalitol.
[0015] In this invention, the tobacco leaf is divided into 14 regions, and further subdivided according to these regions. The method for dividing the 14 regions is as follows: the tobacco leaf is cut along the leaf base towards the leaf tip into 6 regions: leaf base, near leaf base, lower leaf middle, upper leaf middle, near leaf tip, and leaf tip. Then, the four regions of near leaf base, lower leaf middle, upper leaf middle, and near leaf tip are vertically cut into 5 parts each, resulting in 14 regions. The 5 parts are 3 regions near leaf margin, near middle leaf, and near midrib, where the region near midrib is the axis of symmetry, and the parts symmetrical to each other on both sides are the same region. The cutting is done at equal intervals.
[0016] In this invention, the content of tobacco glandular secretions in each location of tobacco leaves is detected to obtain quality characteristic data of tobacco leaves in different locations; the content of tobacco glandular secretions includes the content of α-cephalitol and β-cephalitol, wherein the content of α-cephalitol and β-cephalitol is determined by the method in YC / T470-2013 "Determination of Cephalitol Diterpenoids in Tobacco and Tobacco Products by Gas Chromatography-Mass Spectrometry".
[0017] In this invention, the tobacco leaves to be tested are blown and air-separated to separate tobacco flakes of different shapes. The content of secretions from the trichomes of different shapes of tobacco flakes is detected to obtain quality characteristic data of tobacco flakes of different shapes. The tobacco flakes are classified into large flakes, medium flakes, small flakes, fragments, and dust, wherein large flakes are >25.4mm, medium flakes are ≤25.4mm, 6.35mm are <12.7mm, small flakes are ≤12.7mm, fragments are ≤6.35mm, and dust is ≤2.36mm.
[0018] In this invention, the quality characteristic data of tobacco leaves from different locations obtained in step (2) and the quality characteristic data of tobacco leaves of different leaf types obtained in step (3) are subjected to cluster analysis to obtain the source tracing results of tobacco leaves of different leaf types in tobacco leaf locations; the cluster analysis is performed using SPASS software.
[0019] In this invention, the tobacco leaves are sourced from Xuchang, and the variety of the tobacco leaves is K326.
[0020] This invention provides the application of the method in formulating refined leaf-pruning parameters.
[0021] The present invention also provides the application of the method in improving the utilization rate of tobacco leaves.
[0022] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0023] Example
[0024] 10 kg of intact, undamaged tobacco leaves from the Xuchang production area of the same batch were used as material. The tobacco leaves were cut along the leaf base to the leaf tip into six sections: leaf base, near leaf base, lower leaf middle, upper leaf middle, near leaf tip, and leaf tip. Then, each of the four sections near leaf base, lower leaf middle, upper leaf middle, and near leaf tip was vertically cut into five parts. These five parts were further divided into three sections: near leaf margin, near middle leaf, and near midrib. Using the section near midrib as the axis of symmetry, the parts symmetrical to each other on both sides are considered the same section, resulting in 14 sections. Figure 1 As shown.
[0025] To ensure the representativeness of the test data, the weight of each tobacco leaf from each location was ensured to be greater than 500g. After removing the midrib from the 14 segmented tobacco leaves, they were sorted and stored. Gas chromatography-mass spectrometry (GC-MS) was used to detect the content of trichome secretions (α-citrienol content, β-citrienol content, and total content) in each location. The results of the trichome secretion content detection are shown in Table 1. The trichome secretion content (α-citrienol content and total β-citrienol content) in different locations is as follows: Figure 2 As shown.
[0026] Undamaged tobacco leaves from the Xuchang production area were threshed in the same batch. Cross-sectional samples of 3 kg were taken from the sum of tobacco leaves from each threshing unit. The samples were then sieved and completely separated using a leaf vibration sorting machine to obtain different types of tobacco leaves (where large leaves > 25.4 mm, 12.7 mm < medium leaves ≤ 25.4 mm, 6.35 mm < small leaves ≤ 12.7 mm, 2.36 mm < fragments ≤ 6.35 mm, and dust ≤ 2.36 mm). The different types of tobacco leaves were then classified and stored, with each type weighing 500 g. The content of glandular trichome secretions (α-cipertrienol content, β-cipertrienol content, and total content) in different types of tobacco leaves was determined using the method in YC / T470-2013 "Determination of Cipertrienols in Tobacco and Tobacco Products by Gas Chromatography-Mass Spectrometry". The results of the glandular trichome secretion content are shown in Table 2. Figure 2 As shown.
[0027] The tobacco leaf threshing method includes the following steps: (1) The tobacco leaves are passed through a leaf-beating machine with an opening size of 3-4" diamond-shaped frame for the first stage of leaf beating. The speed of the beating roller is 400~500r / min. The mixture of leaf and stem formed after leaf beating is air-separated to obtain qualified air-separated tobacco leaves and unseparated tobacco leaf mixture containing stems 1. (2) The unseparated tobacco mixture 1 obtained in step (1) is subjected to secondary leaf blasting by a leaf blasting machine with an opening size of 2.5-3" diamond frame, and the blasting roller speed is 600~700r / min. The leaf and stem mixture formed after leaf blasting is air-separated to obtain qualified tobacco and unseparated tobacco mixture 2. (3) The unseparated tobacco mixture 2 obtained in step (2) is subjected to three-stage leaf blasting by a leaf blasting machine with an opening size of 1-2.5" diamond frame and the blasting roller speed is 700~850r / min. The leaf and stem mixture formed after leaf blasting is air-separated to obtain qualified tobacco and unseparated tobacco mixture 3. (4) The unseparated tobacco mixture 3 obtained in step (3) is subjected to four-stage leaf blasting by a leaf blasting machine with a circular frame opening size of Φ40-60mm. The speed of the blasting roller is 800~1000r / min. The leaf and stem mixture formed after leaf blasting is then air-separated to achieve leaf and stem separation. (5) The tobacco flakes obtained after leaf beating and air separation at each level are screened by a leaf structure screening machine to obtain dust, fragments, small flakes, medium flakes and large flakes.
[0028] Table 1. Glandular secretion content (mg / g) of tobacco leaves from different locations
[0029] Table 2. Glandular secretion content (mg / g) of different types of tobacco flakes
[0030] Cluster analysis was performed on the content of glandular trichome secretions in different locations and different types of tobacco leaves to trace the origin of different tobacco leaf types after harvesting. The cluster analysis results are as follows: Figure 3 As shown, the source tracing results of the location of different types of tobacco sheets in tobacco leaves were obtained.
[0031] Depend on Figure 3 It can be seen that the glandular trichome secretions in the 3rd, 2nd, and 5th zones of the leaves from the Xuchang production area are similar to those of medium and small leaves, the glandular trichome secretions in the 1st zone are similar to those of large leaves, the glandular trichome secretions in the 13th zone are similar to those of fragments, and the glandular trichome secretions in the 4th, 8th, 11th, and 6th zones are similar to those of fragments.
[0032] Comparative Example
[0033] Fourteen tobacco leaves of different locations and shapes were obtained using the same method as in the previous example. After removing the midribs, the tobacco leaves of the fourteen locations and different shapes were classified and stored. The content of alkaloids in each location was detected by the method in YC / T559-2018 "Determination of Alkaloids, Characteristic Components of Tobacco, by Gas Chromatography-Mass Spectrometry and Gas Chromatography-Tandem Mass Spectrometry". The results are shown in Table 3.
[0034] Table 3. Neonicotinic acid content (%) in tobacco sheets from different locations and with different sheet types
[0035] Analysis of the nephrine content in tobacco leaves from different locations and with different leaf types revealed that the nephrine content in leaf sections 6, 12, 7, 5, 1, 8, 11, 13, and 2 in the Xuchang production area was the same as that in leaf fragments and dust. Furthermore, the differences in nephrine content between different locations and leaf types were relatively small. This indicates that the nephrine content alone cannot be used to trace the location of tobacco leaves with different leaf types after threshing. Therefore, not all indicators can be used for tracing the location of tobacco leaves with different leaf types after threshing.
[0036] As can be seen from the results of the above embodiments and comparative examples, the present invention provides a method and application for tracing the origin of tobacco flakes based on the analysis of glandular trichome secretion content. The method of the present invention can quickly and accurately determine the location of the tobacco flakes to be tested within the tobacco leaf, providing technical support for the classification and efficient utilization of tobacco flakes. The present invention can also clarify the location of different types of tobacco flakes within the tobacco leaf, as well as the differences in glandular trichome secretion content between tobacco flakes from different locations and between different types of tobacco flakes, laying the foundation for refined leaf threshing and improving the utilization rate of tobacco leaves.
[0037] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. A method for tracing the origin of tobacco leaves based on the analysis of secretions from the glandular trichomes of tobacco leaves, characterized in that, Includes the following steps: (1) Divide the tobacco leaves into 14 regions and further subdivide them according to their regions; (2) The content of glandular trichome secretions in tobacco leaves in each location was detected to obtain the quality characteristics data of tobacco leaves in different locations; (3) The tobacco leaves to be tested were blown and separated into different types of tobacco flakes. The content of secretions from the trichomes of different types of tobacco flakes was detected to obtain quality characteristic data of different types of tobacco flakes. (4) Perform cluster analysis on the quality characteristic data of tobacco leaves from different locations obtained in step (2) and the quality characteristic data of tobacco leaves of different types obtained in step (3) to obtain the source tracing results of tobacco leaves of different types in tobacco leaf locations; The content of the secretions from the cilia includes the content of α-cephalitol and β-cephalitol.
2. The method according to claim 1, characterized in that, The method for dividing the tobacco leaves in step (1) is as follows: the tobacco leaves are divided into six regions along the leaf base to the leaf tip: leaf base, near leaf base, lower leaf middle, upper leaf middle, near leaf tip and leaf tip. Then, the four regions of near leaf base, lower leaf middle, upper leaf middle and near leaf tip are vertically divided into five parts to obtain 14 regions. The five parts are three regions: near the leaf margin, near the middle leaf, and near the midrib. The region near the midrib is the axis of symmetry, and the parts symmetrical to each other on both sides are the same region.
3. The method according to claim 2, characterized in that, The slicing is done at equal intervals.
4. The method according to claim 1, characterized in that, The tobacco leaves in question originated from Xuchang.
5. The method according to claim 1, characterized in that, The tobacco flakes are categorized into large flakes, medium flakes, small flakes, fragments, and dust, with the following dimensions: large flakes > 25.4 mm, medium flakes < 25.4 mm, small flakes < 12.7 mm, fragments < 6.35 mm, and dust ≤ 2.36 mm.
6. The application of the method according to any one of claims 1 to 5 in formulating refined leaf-cutting parameters.
7. The application of the method according to any one of claims 1 to 5 in improving the utilization rate of tobacco leaves.