Preparation system of brewer's grain polypeptide

Abstract

A brewer's grain polypeptide preparation system comprises an alkaline extraction unit, an enzymolysis unit and a separation and purification unit which are communicated in sequence, the alkaline extraction unit at least comprises a tank body and a microwave generator, the tank body can be used for storage reaction of mixed feed liquid of brewer's grains and a solvent, and the microwave generator provides irradiation microwaves. Microwaves output by the microwave generator can perform microwave irradiation treatment on the feed liquid in the tank body; the enzymolysis unit is used for fully hydrolyzing the protein subjected to microwave-assisted alkali extraction in the alkali extraction unit to obtain a peptide solution; and the separation and purification unit is used for performing centrifugal separation and freezing ultrafiltration on the peptide solution to obtain a refined peptide solution. According to the utility model, the protein in the brewer's grains is extracted by adopting microwave-assisted alkali, the heating speed is high, the temperature field is uniform, the extraction time is short, and the protein is fully dissolved out; a protein solution is respectively hydrolyzed by using various proteases, so that the conversion rate of the peptide and the richness of the peptide fragment of the product are improved; and separating and purifying the product peptide solution to finally prepare the brewer's grain polypeptide product with the purity of 95% or above.

Description

Technical Field

[0001] The utility model belongs to the technical field of brewer's grain resource utilization, in particular to a brewer's grain polypeptide preparation system. Background Art

[0002] Brewer's grains are the most important by-product of breweries. Every 1,000 liters of beer produced produces 250 kg of wet grains. Beer wet grains are rich in protein (about 30% based on dry matter), amino acids and trace elements. Currently, they are mostly used as raw materials for microbial fermentation and for making roughage for ruminants, with little economic benefit.

[0003] Due to the extremely high nutritional value and powerful skin care and beauty effects of peptide products, they are widely used in cosmetics, medicine and food, becoming a high value-added product; and beer grains contain barley protein, malt protein and yeast protein. After the amino acid structures of the three proteins complement each other, their nutritional value is almost perfect. In order to effectively utilize beer grains, it is considered to use beer grains for the preparation of peptide products.

[0004] However, the existing process of preparing peptides from brewer's grains is complicated, the extraction time is long, and the purity of the resulting peptide products is low. How to design a simple, practical and effective process route that can efficiently produce high-purity peptide products remains a technical problem that needs to be solved urgently. Utility Model Content

[0005] In view of the above technical problems, the purpose of the utility model is to provide a preparation system of brewer's grains polypeptides, which adopts microwave-assisted alkali extraction of protein in brewer's grains, with fast heating speed, uniform temperature field, short extraction time and sufficient protein dissolution; and the protein solution is hydrolyzed with a variety of proteases to improve the conversion rate of peptides and the richness of product peptide segments; and the resultant peptide solution is separated and purified to finally obtain a brewer's grains polypeptide product with a purity of more than 95%. The application provides a new solution for efficiently preparing high-purity peptide products from brewer's grains, and at the same time provides a good way and method for the recycling of brewer's grains.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0007] A preparation system for brewer's grains polypeptide comprises an alkali extraction unit, an enzymatic hydrolysis unit and a separation and purification unit which are connected in sequence.

[0008] The alkali extraction unit at least comprises a tank body for storing and reacting a mixed liquid of brewer's grains and a solvent, and a microwave generator for irradiating microwaves, wherein the microwaves output by the microwave generator can irradiate the liquid in the tank body with microwaves;

[0009] The enzymatic hydrolysis unit is used to fully hydrolyze the protein extracted by microwave-assisted alkali in the alkali extraction unit to obtain a peptide solution;

[0010] The separation and purification unit is used to perform centrifugal separation and cryo-ultrafiltration on the peptide solution to obtain a refined peptide solution.

[0011] In some technical solutions, a plurality of the microwave generators are disposed on the outside of the tank body and are evenly arranged along the circumference and height directions of the tank body.

[0012] In some technical solutions, the tank body is equipped with a stirring device, which includes a stirring assembly and a power device. The stirring assembly includes a stirring shaft passing through the tank body and a stirring paddle connected to the stirring shaft. The power device drives the stirring shaft to drive the stirring paddle to rotate.

[0013] In some technical solutions, the preparation system further includes a box body mounted on the outside of the tank body, the microwave generator is arranged in the interlayer space between the tank body and the box body, and the box body is used to shield the output microwaves of the microwave generator.

[0014] In some technical solutions, the upper end of the box is provided with a feeding port for inputting the feed liquid and the cleaning liquid, and the lower end of the box is provided with a feeding port for outputting the protein solution;

[0015] The upper end of the tank body is open, and the lower end is provided with a discharge port for discharging the dissolved protein solution;

[0016] The feeding port is located above the tank body, and the taking port is located below the tank body.

[0017] In some technical solutions, the tank body is fixedly connected to the inner side wall of the box body through one or more fixing rods installed on the outer side wall and arranged along the radial direction of the tank body.

[0018] In some technical solutions, the enzymatic hydrolysis unit includes at least one enzymatic hydrolysis reaction tank for hydrolyzing protein with alkaline protease and neutral protease to form a peptide solution, and the enzymatic hydrolysis reaction tank is equipped with a temperature regulating device.

[0019] In some technical schemes, the separation and purification unit includes a centrifugal separation device, a refrigeration device and an ultrafiltration device arranged in sequence. The centrifugal separation device is provided with multiple stages to remove particulate impurities and a small amount of fat in the peptide solution. The ultrafiltration device is used to further remove a small amount of starch, dextrin and fine fiber particles precipitated after freezing to obtain a refined peptide solution.

[0020] In some technical solutions, the preparation system further comprises a concentration and drying unit, which is disposed downstream of the separation and purification unit and is used to concentrate the peptide solution under suitable environmental conditions and to obtain a brewer's grains polypeptide product by spray drying.

[0021] In some technical solutions, the preparation system further comprises an impurity removal unit connected in series between the alkali extraction unit and the enzymatic hydrolysis unit, wherein the impurity removal unit is used to filter and purify the protein solution obtained by the alkali extraction unit to obtain a clarified protein solution; and / or,

[0022] The preparation system further comprises a peptide segment classification unit connected in series between the separation and purification unit and the concentration and drying unit, wherein the peptide segment classification unit comprises an ultrafiltration device for separating polypeptides and oligopeptides.

[0023] The utility model adopts the above technical solution to have at least the following beneficial effects:

[0024] 1. The utility model provides a preparation system of brewer's grain polypeptide, which uses microwave-assisted alkali to extract protein from brewer's grain. The temperature rises quickly, the temperature field is uniform, the extraction time is short, and the protein is fully dissolved. In this case, by detecting the protein content in brewer's grain before the reaction, brewer's grain after the reaction, and the reaction liquid (Kjeldahl nitrogen determination method), it can be obtained that more than 75% of the protein in the brewer's grain is dissolved in the reaction liquid;

[0025] 2. The utility model provides a preparation system of brewer's grain polypeptides. By evenly distributing microwave generators on the outer circumference of the tank body, the brewer's grains and pure water contained in the tank body are evenly irradiated with microwaves, and a uniform temperature field is quickly formed inside the tank body, which assists the alkali solution to dissolve the protein efficiently and with high content.

[0026] 3. The utility model provides a preparation system of brewer's grains polypeptides. In the enzymatic hydrolysis process, the protein solution is hydrolyzed with alkaline protease and neutral protease in sequence, and the appropriate reaction temperature and time are controlled to fully hydrolyze the protein to obtain a peptide solution, and the molecular weight value of the peptide segment in the peptide solution is enriched;

[0027] 4. The utility model provides a preparation system of brewer's grains peptides, which separates, purifies and inactivates the peptide solution obtained by full hydrolysis, and completely removes fine particles and fat in the peptide solution through multi-stage centrifugal separation and freezing ultrafiltration to obtain a refined peptide solution. The peptide content in the refined peptide solution is more than 95% as detected by an authoritative organization;

[0028] 5. The utility model provides a preparation system of brewer's grains polypeptides, which can obtain peptide products of different types and uses by screening and distinguishing the purified peptide solutions of peptide segments with different molecular weights. Specifically, polypeptide products and oligopeptide products can be obtained with the molecular weight of 2000 Da1 as the boundary;

[0029] 6. The utility model provides a preparation system for brewer's grains polypeptides, which can concentrate and dry the peptide solution to produce a powder product, which is convenient for subsequent transportation and storage. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings and their marks required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0031] Figure 1 A cross-sectional view of the alkali extraction unit according to an embodiment of the utility model from a front perspective;

[0032] Figure 2 It is a top view of the alkali extraction unit described in an embodiment of the utility model.

[0033] The meanings of the symbols in the figure are as follows:

[0034] 1—power equipment, 2—stirring shaft, 3—box, 4—microwave generator, 5—fixed rod, 6—tank body, 7—stirring paddle, 8—discharging port, 9—feeding port, 10—feeding port. DETAILED DESCRIPTION

[0035] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the specific implementation methods of the present invention will be described below with reference to the accompanying drawings. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings and other implementation methods can be obtained based on these drawings without creative work.

[0036] In order to simplify the drawings, only the parts related to the invention are schematically shown in each figure, and they do not represent the actual structure of the product. In addition, in order to simplify the drawings and facilitate understanding, in some figures, only one of the parts with the same structure or function is schematically drawn or marked. In this article, "one" not only means "only one", but also means "more than one".

[0037] It should be further understood that the term “and / or” used in the specification and appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0038] In this document, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0039] In addition, in the description of the present application, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0040] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the presently described subject matter belongs.

[0041] In one embodiment, the utility model provides a preparation system of brewer's grains polypeptide, please refer to Figure 1 Place Figure 2 , including an alkali extraction unit, an enzymatic hydrolysis unit and a separation and purification unit connected in sequence, the alkali extraction unit at least includes a tank body 6 for storing and reacting a mixed liquid of brewer's grains and a solvent, and a microwave generator 4 for providing microwave irradiation, the microwave output by the microwave generator 4 can perform microwave irradiation treatment on the liquid in the tank body 6; the enzymatic hydrolysis unit is used to fully hydrolyze the protein extracted by microwave-assisted alkali in the alkali extraction unit to obtain a peptide solution; the separation and purification unit is used to centrifuge and freeze-ultrafilter the peptide solution to obtain a refined peptide solution.

[0042] In the present application, the characteristic of plant protein being easily soluble in an alkaline environment is utilized to extract protein from brewer's grains using alkaline solution, thereby achieving a high protein extraction rate and extracted protein purity. At the same time, microwaves are utilized to assist in protein dissolution. Since microwave equipment heats up quickly and has a uniform temperature field, the extraction time of protein from brewer's grains is significantly shortened. At the same time, the operation is simple, and ethanol solvent extraction is avoided, so that no flammable and explosive materials are produced in the production workshop, a large amount of ethanol resources are saved, and peptides in most ethanol-insoluble proteins can be extracted.

[0043] In the above embodiment, the alkali extraction unit includes a box body 3 and a built-in tank body 6, and the microwave generator 4 is arranged in the interlayer space between the tank body 6 and the box body 3, and the box body 3 is used to shield the output microwaves of the microwave generator 4; preferably, multiple microwave generators are evenly arranged along the circumference and height directions of the tank body 6.

[0044] In a specific embodiment, an upper microwave generator and a lower microwave generator are respectively arranged at different heights on the outer circumference of the tank body, wherein the number of the upper microwave generators and the lower microwave generators are 3 and are evenly spaced; preferably, the upper microwave generator is 30 cm away from the top of the tank body 6, and the lower microwave generator is 20 cm away from the bottom of the tank body 6, and the power of the upper microwave generator and the lower microwave generator is 1000 W.

[0045] In this embodiment, the microwave generators 4 are evenly arranged on the outer circumference of the tank body 6, so that the brewer's grains and pure water contained in the tank body 6 are evenly irradiated with microwaves, and a uniform temperature field is quickly formed inside the tank body 6, which assists the alkaline solution in dissolving protein efficiently and with high content.

[0046] In a preferred embodiment, the tank body 6 is equipped with a stirring device, which includes a stirring assembly and a power device 1. The stirring assembly includes a stirring shaft 2 that passes through the tank body 6 and a stirring paddle 7 connected to the stirring shaft 2. The power device 1 is arranged outside the box body 3 to drive the stirring shaft 2 to drive the stirring paddle 7 to rotate.

[0047] In this embodiment, during the microwave-assisted alkaline dissolution of protein in brewer's grains, a stirring device is used to stir the slurry inside the tank body so that the slurry and the added reagents are fully mixed, thereby avoiding insufficient protein dissolution caused by uneven slurry concentration and further increasing the amount of protein dissolved in brewer's grains.

[0048] In a specific embodiment, a feeding port 9 for inputting feed liquid and cleaning liquid is provided at the upper end of the box body 3, and a feeding port 10 for outputting protein solution is provided at the lower end of the box body 3; the upper end of the tank body 6 is open, and a discharge port 8 for discharging the dissolved protein solution is provided at the lower end; the feeding port 9 is located above the tank body 6, and the feeding port 10 is located below the tank body 6.

[0049] In a specific embodiment, the tank body 6 is fixedly connected to the inner side wall of the box body 3 via one or more fixing rods 5 installed on the outer side wall and arranged along the radial direction of the tank body.

[0050] It should be pointed out that, for the relatively fixed structure between the tank body 6 and the box body 3, this article gives the above-mentioned specific implementation method. Of course, it should include a variety of simple transformation implementation plans, that is, the structural design that satisfies the relatively fixed connection between the outer wall of the tank body and the inner wall of the box body 3 should be included in the protection scope of this application without departing from the concept of the utility model.

[0051] In the above embodiment, the enzymatic hydrolysis unit includes at least one enzymatic hydrolysis reaction tank for alkaline protease and neutral protease to hydrolyze protein to form a peptide solution. The enzymatic hydrolysis reaction tank is equipped with a temperature regulating device. Of course, it should also be equipped with the above-mentioned stirring device to meet the control requirements of specific reaction environment conditions.

[0052] Preferably, an impurity removal unit is provided between the alkali extraction unit and the enzymatic hydrolysis unit, and the impurity removal unit is used to filter and purify the protein solution obtained by the alkali extraction unit to obtain a clarified protein solution. Specifically, the impurity removal unit includes a screen and a plate-and-frame filter press arranged in series.

[0053] In the above embodiment, the separation and purification unit includes a centrifugal separation device, a refrigeration device and an ultrafiltration device arranged in sequence. The centrifugal separation device is provided with multiple stages to remove particulate impurities and a small amount of fat in the peptide solution. The ultrafiltration device is used to further remove a small amount of starch, dextrin and fiber fine particles precipitated after freezing to obtain a refined peptide solution.

[0054] Specifically, the multi-stage centrifugal separation equipment includes a GF-150 tubular centrifuge and a GF-105 tubular centrifuge connected in sequence, the GF-150 tubular centrifuge removes fine particulate impurities at a speed of 13000RPM, and the GF-105 tubular centrifuge removes a small amount of fat in the peptide solution at a speed of 15000RPM; the refrigeration equipment is a freezer; the ultrafiltration equipment is an ultrafiltration machine, which can filter out impurities with a molecular weight of more than 5000Dal; the peptide content of the peptide solution after separation and purification is more than 95%.

[0055] In the above embodiment, the preparation system further includes a concentration and drying unit, which is disposed downstream of the separation and purification unit and is used to concentrate the peptide solution under suitable environmental conditions and spray dry it to obtain a brewer's grains polypeptide product.

[0056] Preferably, a peptide classification unit is connected in series between the separation and purification unit and the concentration and drying unit, and the peptide classification unit includes an ultrafiltration device, which is used to separate polypeptides and oligopeptides.

[0057] In order to have a clearer understanding of the technical solution and technical effects of this application, the following specific process flow examples are provided:

[0058] Microwave-assisted alkali separation of the protein in the brewer's grains is used, and the protein solution is enzymatically hydrolyzed with alkaline protease and neutral protease in sequence to obtain a peptide solution. The peptide solution is sequentially purified, refined, concentrated and dried to obtain a brewer's grains polypeptide product.

[0059] In the above example, the following process steps are implemented and relevant process parameters are controlled during microwave-assisted alkali separation of protein from brewer's grains:

[0060] 30 kg of brewer's grains and 60 kg of pure water are input into the tank 6 for mixing. In the implementation, the feed liquid is preferably changed between 1: (1-3). 15% sodium hydroxide solution is added dropwise under stirring to adjust the pH value of the feed liquid to 9-11. The microwave irradiation reaction is started under stirring at 100 rpm. The treatment time is 30-40 minutes and the treatment temperature is 40-55° C. The entire extraction takes 30 minutes in summer, 40 minutes in winter, and the reaction time in spring and autumn is between the two.

[0061] This is the best reaction time obtained after many experiments. If the time is too short, the protein dissolution effect will be poor; if the time is too long, the temperature of the liquid will be too high, causing protein denaturation. The purpose of this microwave reaction is to assist the alkali solution to fully dissolve the protein in the brewer's grains in the water. After testing the protein content in the brewer's grains before the reaction, the brewer's grains after the reaction, and the reaction liquid (Kjeldahl nitrogen determination method), this reaction allows more than 75% of the protein in the brewer's grains to dissolve into the reaction liquid.

[0062] In the above example, the following process steps are performed and relevant process parameters are controlled in the process of enzymatically hydrolyzing the protein solution with alkaline protease and neutral protease to obtain a peptide solution:

[0063] The protein solution after impurity removal is input into the enzymatic hydrolysis reaction tank, and alkaline protease with a mass concentration of 0.1-0.3% is first added into the enzymatic hydrolysis reaction tank and stirred at 40-50°C for 1-2 hours;

[0064] 10% hydrochloric acid was added to adjust the pH to 7, and then 0.1-0.3% neutral protease was added to the enzymatic reaction tank and stirred at 40-50° C. for 1-2 hours to obtain a peptide solution.

[0065] In this embodiment, during the enzymatic hydrolysis process, the protein solution is hydrolyzed with alkaline protease and neutral protease in sequence, and the appropriate reaction temperature and time are controlled to fully hydrolyze the protein to obtain a peptide solution, and the molecular weight values ​​of the peptide segments in the peptide solution are enriched.

[0066] In some preferred embodiments, the impurity removal process of the above protein solution is specifically as follows:

[0067] The feed solution after microwave-assisted dissolution treatment was first filtered through a 200-mesh stainless steel sieve to remove coarse residues, and then filtered through a plate and frame filter press to obtain a clarified protein solution.

[0068] In this embodiment, fine particles and colloidal impurities in the protein dissolved from brewer's grains can be effectively removed, ensuring the clarity of the protein solution entering the enzymatic hydrolysis reaction.

[0069] In the above example, the steps of purifying and refining the peptide solution are to use centrifugal separation to remove particulate impurities and a small amount of fat in the peptide solution to obtain a defatted liquid; and then freeze-ultrafiltration to obtain a refined peptide solution.

[0070] Specifically, the following steps are included:

[0071] First, the peptide solution was input into a GF-150 tubular centrifuge at 13000 RPM to remove fine particle impurities;

[0072] Then, the solution was centrifuged in a GF-105 tube at 15,000 RPM to remove a small amount of fat in the peptide solution.

[0073] The defatted liquid is then frozen and filtered with an ultrafiltration membrane to remove impurities with a molecular weight of more than 5000 Da1, and the rest is collected. This removes the protease and other impurities previously added, leaving a high-purity peptide solution.

[0074] The fine particles and fat in the peptide solution can be completely removed through the purification and refining steps of the peptide solution above. The applicant commissioned a professional testing agency to conduct relevant chromatography-mass spectrometry tests on the purified and refined peptide solution above. The peptide content in the obtained solution was above 95%, and the impurity residue was relatively low. For details, please refer to the peptide solution composition table in the table below:

[0075] Testing environment conditions: temperature 23±3℃, humidity 55±5%RH

[0076]

[0077] Through the above enzymatic hydrolysis process, the protein in the brewer's grains is converted into peptides of different molecular weight ranges. According to the application requirements of related industries, they can be classified to make products of different molecular weights. Specifically, ultrafiltration membrane is used for filtration. The ultrafiltration uses the molecular weight of 2000Dal as the boundary. Those above 2000Dal are polypeptide products, and the rest are oligopeptide products, producing two products.

[0078] In the above example, the following process steps are implemented and relevant process parameters are controlled during the process of concentrating and drying the refined peptide solution to obtain the brewer's grains polypeptide product:

[0079] The purified peptide solution is concentrated under vacuum conditions of -81 to -76 KPa and temperature of 60 to 65°C until the solid content reaches 40 to 42%, and then spray dried to obtain a powder product. The spray drying air inlet temperature is 180-200°C and the air outlet temperature is about 70°C.

[0080] The present application combines microwave-assisted alkaline dissolution of proteins in brewer's grains, sequential enzymatic hydrolysis of the protein solution with a combination of proteases, and purification and refinement of the obtained peptide solution, thereby improving the protein dissolution efficiency and protein amount from the source, strictly controlling the impurity content and enriching the product diversity during production, and efficiently and thoroughly removing various fine impurities after production, ultimately efficiently obtaining high-purity peptide products, which can be widely used in the fields of cosmetics, medicine, and food, achieving effective utilization of waste brewer's grains resources, and obtaining higher economic value.

[0081] It should be pointed out that the raw materials for preparing the polypeptide of the present application include but are not limited to distiller's grains and lees, which include both the lees remaining after brewing wine with grains such as rice and wheat, and the milky white residue filtered out when brewing sake. Solid waste residues containing usable protein in the preparation process of other wines can also be selected, which can be wet residues or dry residues. Without exceeding the concept of the present invention, schemes involving raw material replacement and change should be within the scope of protection of this application.

[0082] The above-mentioned embodiments only express several implementation methods of the utility model, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the patent of the utility model. It should be pointed out that, for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, and these all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model shall be based on the attached claims.

Claims

1. A system for preparing brewer's grains polypeptide, characterized in that: It includes an alkali extraction unit, an enzymatic hydrolysis unit and a separation and purification unit which are connected in sequence. The alkali extraction unit at least comprises a tank body for storing and reacting a mixed liquid of brewer's grains and a solvent, and a microwave generator for irradiating microwaves, wherein the microwaves output by the microwave generator can irradiate the liquid in the tank body with microwaves; The enzymatic hydrolysis unit is used to fully hydrolyze the protein extracted by microwave-assisted alkali in the alkali extraction unit to obtain a peptide solution; The separation and purification unit is used to perform centrifugal separation and cryo-ultrafiltration on the peptide solution to obtain a refined peptide solution.

2. The preparation system according to claim 1, characterized in that: A plurality of microwave generators are arranged on the outside of the tank body and are evenly arranged along the circumference and height directions of the tank body.

3. The preparation system according to claim 1 or 2, characterized in that: The tank body is equipped with a stirring device, which includes a stirring assembly and a power device. The stirring assembly includes a stirring shaft penetrating the tank body and a stirring paddle connected to the stirring shaft. The power device drives the stirring shaft to drive the stirring paddle to rotate.

4. The preparation system according to claim 1 or 2, characterized in that: The preparation system further comprises a box body which is sleeved on the outside of the tank body, the microwave generator is arranged in the interlayer space between the tank body and the box body, and the box body is used for shielding the output microwave of the microwave generator.

5. The preparation system according to claim 4, characterized in that: The upper end of the box is provided with a feeding port for inputting feed liquid and cleaning liquid, and the lower end of the box is provided with a feeding port for outputting protein solution; The upper end of the tank body is open, and the lower end is provided with a discharge port for discharging the dissolved protein solution; The feeding port is located above the tank body, and the taking port is located below the tank body.

6. The preparation system according to claim 4, characterized in that: The tank body is fixedly connected to the inner side wall of the box body through one or more fixing rods installed on the outer side wall and arranged along the radial direction of the tank body.

7. The preparation system according to claim 1, characterized in that: The enzymolysis unit at least comprises an enzymolysis reaction tank for hydrolyzing protein with alkaline protease and neutral protease to form a peptide solution, and the enzymolysis reaction tank is equipped with a temperature regulating device.

8. The preparation system according to claim 1, characterized in that: The separation and purification unit includes a centrifugal separation device, a refrigeration device and an ultrafiltration device arranged in sequence. The centrifugal separation device is provided with multiple stages to remove particulate impurities and a small amount of fat in the peptide solution. The ultrafiltration device is used to further remove a small amount of starch, dextrin and fine fiber particles precipitated after freezing to obtain a refined peptide solution.

9. The preparation system according to claim 8, characterized in that: The preparation system also includes a concentration and drying unit, which is arranged downstream of the separation and purification unit and is used to concentrate the peptide solution under suitable environmental conditions and spray dry it to obtain a brewer's grains polypeptide product.

10. The preparation system according to claim 9, characterized in that: The preparation system further comprises an impurity removal unit connected in series between the alkali extraction unit and the enzymatic hydrolysis unit, wherein the impurity removal unit is used to filter and purify the protein solution obtained by the alkali extraction unit to obtain a clarified protein solution; and / or, The preparation system further comprises a peptide segment classification unit connected in series between the separation and purification unit and the concentration and drying unit, wherein the peptide segment classification unit comprises an ultrafiltration device for separating polypeptides and oligopeptides.

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