Detection equipment for quantitative sample adding of mRNA stock solution in vitro detection

Abstract

The utility model relates to the technical field of active cell detection, and discloses detection equipment for quantitative sample adding of mRNA (messenger Ribonucleic Acid) stock solution in vitro detection, which comprises a working table, a first limiting plate is fixedly connected to the upper surface of the working table, a support plate is fixedly connected to the upper surface of the first limiting plate, a liquid outlet pipe is fixedly connected to the outer wall of the support plate, and a liquid outlet pipe is fixedly connected to the outer wall of the liquid outlet pipe. A dropper is fixedly connected to the lower surface of the liquid outlet pipe, a frame is fixedly connected to the upper surface of the first limiting plate, a hydraulic machine is fixedly connected to the outer wall of the frame, a pushing block is fixedly connected to the output end of the hydraulic machine, and a quantitative pipe is fixedly connected to the outer wall of the pushing block. According to the device, the hydraulic machine is started to drive the pushing block to move, so that the quantitative tube is driven to move, the quantitative tube moves to drive the second rotating column to move, the quantitative tube also drives the sliding plate to move at the same time, the sliding plate moves to drive the baffle to move, the effect of quantitatively adding an mRNA solution is achieved, and the effect of preventing cell inactivation caused by too much or too little mRNA solution adding is achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of active cell detection, in particular to a detection device for quantitatively adding samples of mRNA stock solution in in vitro activity detection. Background Art

[0002] mRNA (messenger RNA) is a type of molecule that carries genetic information in cells. Its function in organisms is mainly to transfer genetic information in DNA to the cytoplasm and guide protein synthesis. The synthesis of mRNA begins with genes on DNA, and the genetic information is transcribed into mRNA through the transcription process. After transcription, mRNA may undergo a splicing process, in which the combination of non-coding regions (non-coding sub-regions) and coding regions (coding sub-regions) may change. The activity of mRNA is directly related to the regulation of gene expression. Cells achieve precise control of gene expression by regulating the synthesis, stability and translation of mRNA. Therefore, studying the activity of mRNA helps to gain a deeper understanding of the gene regulatory network, so a detection device for quantitative addition of mRNA stock solution in in vitro activity measurement is proposed.

[0003] After searching, the announcement number: CN217997165U discloses a detection device for quantitatively adding mRNA stock solution in vitro detection of large pore mites, including a detection box, the outer wall of the detection box is rotatably connected to an outer cover, the top of the inner wall of the detection box is equipped with a detection device body, and the inner wall of the detection box is provided with vertical and horizontal guide rails; the top of the vertical and horizontal guide rails is threadedly connected to a mounting plate, the upper surface of the mounting plate is slidably connected to a culture table, the upper surface of the mounting plate is provided with an inner groove at the bottom of the culture table, one end of the lower surface of the culture table is fixedly connected to a tail support, the tail support is inserted into the bottom of the inner wall of the inner groove, the inside of the inner groove is rotatably connected to a lifting rod, the other end of the lower surface of the culture table is fixedly connected to a round head rod, one end of the round head rod is clamped on the outer wall of the lifting rod. The utility model can save the time of disassembly and assembly of the culture table, and can take out the upper group of culture tables that have been tested from the bracket surface when testing the lower group of culture tables, so as to save the detection time during large-scale testing.

[0004] The above application can save the time for disassembly and assembly of the culture platform, and can remove the tested upper group culture platform from the surface of the bracket when testing the lower group culture platform, so as to save the testing time for large-scale testing. However, during use, it is not taken into account that during the test, a trace amount of detection solution needs to be added. When it is added manually, too much or too little liquid may be added, resulting in cell death or distorted results. Utility Model Content

[0005] In order to make up for the above shortcomings, the utility model provides a detection device for quantitative addition of mRNA stock solution in in vitro activity detection, aiming to improve the problem that the need to add mRNA solution during detection is not taken into account, and when it is added manually, too much or too little liquid may be added, resulting in cell death or distorted results.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] A method for detecting mRNA activity in vitro based on a cell method comprises a workbench, wherein a first limiting plate is fixedly connected to the upper surface of the workbench, a support plate is fixedly connected to the upper surface of the first limiting plate, a liquid outlet pipe is fixedly connected to the outer wall of the support plate, a dripper is fixedly connected to the lower surface of the liquid outlet pipe, a frame is fixedly connected to the upper surface of the first limiting plate, a hydraulic press is fixedly connected to the outer wall of the frame, a pushing block is fixedly connected to the output end of the hydraulic press, a quantitative tube is fixedly connected to the outer wall of the pushing block, a second rotating column is rotatably connected to the interior of the quantitative tube, a rotating block is fittedly connected to the outer wall of the second rotating column, an outer wall of the rotating block is rotatably connected to the outer wall of the pushing block, a slide plate is fixedly connected to the upper surface of the quantitative tube, an outer wall of the slide plate is slidably connected to the inner wall of the frame, a baffle is fixedly connected to the upper surface of the slide plate, a fixed tube is fixedly connected to the upper surface of the frame, a liquid storage tank is fixedly connected to the interior of the fixed tube, and a rotating assembly is provided on the upper surface of the workbench.

[0008] Preferably, the rotating assembly comprises a motor, the lower surface of the motor is fixedly connected to the upper surface of the workbench, the output end of the motor is fixedly connected to a turntable, and the inner wall of the turntable is slidably connected to a test tube.

[0009] Preferably, a load-bearing plate is fixedly connected to the lower surface of the workbench, and a first hollow column is fixedly connected to the lower surface of the load-bearing plate.

[0010] Preferably, the inner wall of the first hollow column is slidably connected to a sliding column, and the lower surface of the sliding column is fixedly connected to a bottom plate.

[0011] Preferably, a second hollow column is fixedly connected to the upper surface of the bottom plate, and a second limiting plate is fixedly connected to the outer wall of the second hollow column.

[0012] Preferably, the outer wall of the second limiting plate is fixedly connected to the third limiting plate, the inner wall of the third limiting plate is rotatably connected to a crank, and one end of the crank is fixedly connected to the first rotating column.

[0013] Preferably, one end of the first rotating column is fixedly connected to a first bevel gear, and an outer wall of the first bevel gear is meshingly connected to a second bevel gear.

[0014] Preferably, the outer wall of the second bevel gear is rotatably connected to the inner wall of the second hollow column, the inner wall of the second bevel gear is threadedly connected to a threaded column, and the upper surface of the threaded column is rotatably connected to the lower surface of the load-bearing plate.

[0015] The utility model has the following beneficial effects:

[0016] 1. In the utility model, the hydraulic press is started, the hydraulic press drives the push block to move, the movement of the push block drives the quantitative tube to move, the movement of the quantitative tube drives the second rotating column to move, the movement of the quantitative tube also drives the slide plate to move, the movement of the slide plate drives the baffle plate to move, when the quantitative tube moves to the liquid outlet tube, the baffle plate will block the fixed tube, so as to achieve the effect of quantitatively adding the mRNA solution, and prevent the effect of adding too much or too little to cause cell death or distorted results.

[0017] 2. In the utility model, when the crank is turned, the crank drives the first rotating column to rotate, the rotation of the first rotating column drives the first bevel gear to rotate, the rotation of the first bevel gear drives the second bevel gear to rotate, the rotation of the second bevel gear drives the threaded column to move, the movement of the threaded column drives the load-bearing plate to move, and the movement of the load-bearing plate drives the workbench to move, so that the height of the workbench can be adjusted according to the height of the researcher, preventing the researcher from bending over for a long time and damaging his health.

[0018] 3. In the utility model, the hydraulic press drives the push block to move, and the push block drives the quantitative tube to move. The mRNA solution flowing out of the quantitative tube is added to the test tube, and then the crank is turned to drive the first bevel gear and the second bevel gear to rotate, driving the threaded column to move, thereby improving the work efficiency of researchers. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a stereoscopic diagram of the detection device for quantitatively adding samples of mRNA stock solution in in vitro activity detection proposed by the utility model;

[0020] Figure 2 A schematic diagram of a dripper of a detection device for quantitatively adding mRNA stock solution in an in vitro activity test proposed by the utility model;

[0021] Figure 3 This is a schematic diagram of the first bevel gear of the detection device for quantitatively adding mRNA stock solution in in vitro activity detection proposed by the utility model.

[0022] Legend:

[0023] 1. Workbench; 2. First limit plate; 3. Support plate; 4. Liquid outlet pipe; 5. Drip head; 6. Frame; 7. Hydraulic press; 8. Push block; 9. Dosing tube; 10. Slide plate; 11. Rotating block; 12. Baffle plate; 13. Fixed tube; 14. Liquid storage tank; 15. Motor; 16. Turntable; 17. Test tube; 18. Load-bearing plate; 19. First hollow column; 20. Sliding column; 21. Bottom plate; 22. Second hollow column; 23. Second limit plate; 24. Third limit plate; 25. Crank; 26. First rotating column; 27. First bevel gear; 28. Second bevel gear; 29. ​​Threaded column; 30. Second rotating column. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings of the specification of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] Reference Figure 1-Figure 2 The utility model provides an embodiment: a detection device for quantitatively adding mRNA stock solution in vitro activity test, comprising a workbench 1, a first limiting plate 2 is fixedly connected to the upper surface of the workbench 1, a support plate 3 is fixedly connected to the upper surface of the first limiting plate 2, a liquid outlet pipe 4 is fixedly connected to the outer wall of the support plate 3, a dripper 5 is fixedly connected to the lower surface of the liquid outlet pipe 4, a frame 6 is fixedly connected to the upper surface of the first limiting plate 2, a hydraulic press 7 is fixedly connected to the outer wall of the frame 6, a push block 8 is fixedly connected to the output end of the hydraulic press 7, and the outer wall of the push block 8 is fixedly connected to the outer wall of the push block 8. A quantitative tube 9 is connected, the interior of the quantitative tube 9 is rotatably connected to a second rotating column 30, the outer wall of the second rotating column 30 is fitted and connected to a rotating block 11, the outer wall of the rotating block 11 is rotatably connected to the outer wall of the pushing block 8, the upper surface of the quantitative tube 9 is fixedly connected to a slide plate 10, the outer wall of the slide plate 10 is slidably connected to the inner wall of the frame 6, the upper surface of the slide plate 10 is fixedly connected to a baffle plate 12, the upper surface of the frame 6 is fixedly connected to a fixed tube 13, the interior of the fixed tube 13 is fixedly connected to a liquid storage tank 14, and a rotating assembly is provided on the upper surface of the workbench 1;

[0026] Specifically, the hydraulic press 7 is started to drive the pushing block 8 and the rotating block 11 to move. When the pushing block 8 moves, it drives the quantitative tube 9 to move. The movement of the rotating block 11 drives the second rotating column 30 to move. When the quantitative tube 9 moves, it also drives the slide plate 10 to move, thereby driving the baffle 12 to move. When the quantitative tube 9 moves to the top of the liquid outlet tube 4, the baffle 12 will block the fixed tube 13. The second rotating column 30 will flip due to the weight of the liquid in the quantitative tube 9, so that the PCR solution in the quantitative tube 9 flows into the liquid outlet tube 4, and then flows into the interior of the test tube 17 through the dripper 5, and the PCR solution is quantitatively added thereto, so as to achieve the effect of quantitatively adding the PCR solution and preventing the inactivation of mRNA cells due to too much or too little PCR solution added.

[0027] Reference Figure 1 The rotating assembly includes a motor 15, the lower surface of the motor 15 is fixedly connected to the upper surface of the workbench 1, the output end of the motor 15 is fixedly connected to a turntable 16, and the inner wall of the turntable 16 is slidably connected to a test tube 17;

[0028] Specifically, the motor 15 is started to drive the turntable 16 to rotate, thereby driving the test tube 17 to move, and the test tube 17 to be tested is rotated to the bottom of the dripper 5, which reduces the need for researchers to add liquid one by one.

[0029] Reference Figure 1 and Figure 3 The lower surface of the workbench 1 is fixedly connected with a load-bearing plate 18, and the lower surface of the load-bearing plate 18 is fixedly connected with a first hollow column 19; the inner wall of the first hollow column 19 is slidably connected with a sliding column 20, and the lower surface of the sliding column 20 is fixedly connected with a bottom plate 21; the upper surface of the bottom plate 21 is fixedly connected with a second hollow column 22, and the outer wall of the second hollow column 22 is fixedly connected with a second limiting plate 23; the outer wall of the second limiting plate 23 is fixedly connected with a third limiting plate 24, and the inner wall of the third limiting plate 24 is rotatably connected with a crank 25, and one end of the crank 25 is fixedly connected with a first rotating column 26; one end of the first rotating column 26 is fixedly connected with a first bevel gear 27, and the outer wall of the first bevel gear 27 is meshingly connected with a second bevel gear 28; the outer wall of the second bevel gear 28 is rotatably connected to the inner wall of the second hollow column 22, and the inner wall of the second bevel gear 28 is threadedly connected with a threaded column 29, and the upper surface of the threaded column 29 is rotatably connected to the lower surface of the load-bearing plate 18;

[0030] Specifically, rotating the crank 25 drives the first rotating column 26 to rotate, and the rotation of the first rotating column 26 drives the first bevel gear 27 to rotate, thereby driving the second bevel gear 28 to rotate, and the rotation of the second bevel gear 28 drives the threaded column 29 to move, thereby driving the load-bearing plate 18 to move, and the movement of the load-bearing plate 18 drives the first hollow column 19 to slide, so that the height of the workbench 1 can be adjusted according to the height of the researcher, thereby preventing the researcher from bending over for a long time and damaging his health.

[0031] Working principle: When the device is needed, start the motor 15 above the workbench 1, the motor 15 drives the turntable 16 to rotate, the turntable 16 rotates and drives the test tube 17 to move, start the hydraulic press 7 fixed by the support plate 3, the frame 6 and the first limit plate 2, the hydraulic press 7 drives the push block 8 and the rotating block 11 to move, the push block 8 moves and drives the quantitative tube 9 to move, the rotating block 11 moves and drives the second rotating column 30 to move, the quantitative tube 9 moves and drives the slide plate 10 to move, the slide plate 10 moves and drives the baffle 12 to move, when the quantitative tube When the quantitative tube 9 moves to the top of the liquid outlet tube 4, the baffle 12 will block the fixed tube 13, and the second rotating column 30 will also flip due to the weight of the liquid in the quantitative tube 9, so that the mRNA solution in the quantitative tube 9 flows into the liquid outlet tube 4, and then flows into the inside of the test tube 17 through the dripper 5. When the quantitative tube 9 is reset, the liquid storage tank 14 will fill the quantitative tube 9 again, and add the mRNA solution to it quantitatively, so as to achieve the quantitative addition of the mRNA solution, thereby preventing the cell death or formation of mRNA cells caused by too much or too little mRNA solution. To achieve the effect of distortion of the result, the crank 25 fixed by the second limiting plate 23 and the third limiting plate 24 is rotated, and the crank 25 drives the first rotating column 26 to rotate, and the rotation of the first rotating column 26 drives the first bevel gear 27 to rotate, and the rotation of the first bevel gear 27 drives the second bevel gear 28 to rotate inside the second hollow column 22, and the rotation of the second bevel gear 28 drives the threaded column 29 to move, and the movement of the threaded column 29 drives the load-bearing plate 18 to move, and the movement of the load-bearing plate 18 drives the first hollow column 19 to slide on the outer wall of the sliding column 20 fixed on the bottom plate 21, so that the height of the workbench 1 can be adjusted according to the height of the researcher, so as to prevent the researcher from bending over for a long time and damaging his health. The equipment can not only achieve the effect of quantitatively adding mRNA solution to prevent the addition of too much or too little mRNA solution to cause cell death of mRNA cells or distortion of the results, but also achieve the effect of adjusting the height of the workbench 1 according to the height of the researcher, so as to prevent the researcher from bending over for a long time and damaging his health, and finally achieve the effect of improving the work efficiency of the researcher.

[0032] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A detection device for quantitatively adding mRNA stock solution in in vitro activity detection, comprising a workbench (1), characterized in that: The upper surface of the workbench (1) is fixedly connected to a first limit plate (2), the upper surface of the first limit plate (2) is fixedly connected to a support plate (3), the outer wall of the support plate (3) is fixedly connected to a liquid outlet pipe (4), the lower surface of the liquid outlet pipe (4) is fixedly connected to a dripper (5), the upper surface of the first limit plate (2) is fixedly connected to a frame (6), the outer wall of the frame (6) is fixedly connected to a hydraulic press (7), the output end of the hydraulic press (7) is fixedly connected to a push block (8), the outer wall of the push block (8) is fixedly connected to a quantitative tube (9), the interior of the quantitative tube (9) is rotatably connected to a A second rotating column (30), the outer wall of the second rotating column (30) is fittedly connected with a rotating block (11), the outer wall of the rotating block (11) is rotatably connected to the outer wall of the pushing block (8), the upper surface of the quantitative tube (9) is fixedly connected with a slide plate (10), the outer wall of the slide plate (10) is slidably connected to the inner wall of the frame (6), the upper surface of the slide plate (10) is fixedly connected with a baffle (12), the upper surface of the frame (6) is fixedly connected with a fixed tube (13), the interior of the fixed tube (13) is fixedly connected with a liquid storage tank (14), and a rotating assembly is arranged on the upper surface of the workbench (1).

2. The detection device for quantitatively adding mRNA stock solution in in vitro activity detection according to claim 1, characterized in that: The rotating assembly comprises a motor (15), the lower surface of the motor (15) is fixedly connected to the upper surface of the workbench (1), the output end of the motor (15) is fixedly connected to a turntable (16), and the inner wall of the turntable (16) is slidably connected to a test tube (17).

3. The detection device for quantitatively adding mRNA stock solution in in vitro activity detection according to claim 2, characterized in that: The lower surface of the workbench (1) is fixedly connected to a load-bearing plate (18), and the lower surface of the load-bearing plate (18) is fixedly connected to a first hollow column (19).

4. The detection device for quantitatively adding mRNA stock solution in in vitro activity detection according to claim 3, characterized in that: The inner wall of the first hollow column (19) is slidably connected to a sliding column (20), and the lower surface of the sliding column (20) is fixedly connected to a bottom plate (21).

5. The detection device for quantitatively adding mRNA stock solution in in vitro activity detection according to claim 4, characterized in that: A second hollow column (22) is fixedly connected to the upper surface of the bottom plate (21), and a second limiting plate (23) is fixedly connected to the outer wall of the second hollow column (22).

6. The detection device for quantitatively adding mRNA stock solution in in vitro activity detection according to claim 5, characterized in that: The outer wall of the second limiting plate (23) is fixedly connected to the third limiting plate (24), the inner wall of the third limiting plate (24) is rotatably connected to a crank (25), and one end of the crank (25) is fixedly connected to a first rotating column (26).

7. The detection device for quantitatively adding mRNA stock solution in in vitro activity detection according to claim 6, characterized in that: One end of the first rotating column (26) is fixedly connected to a first bevel gear (27), and the outer wall of the first bevel gear (27) is meshingly connected to a second bevel gear (28).

8. The detection device for quantitatively adding mRNA stock solution in in vitro activity detection according to claim 7, characterized in that: The outer wall of the second bevel gear (28) is rotatably connected to the inner wall of the second hollow column (22), and the inner wall of the second bevel gear (28) is threadedly connected to a threaded column (29), and the upper surface of the threaded column (29) is rotatably connected to the lower surface of the bearing plate (18).

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