Stem cell conditioned medium filtering device

Abstract

The utility model relates to the technical field of biology, and discloses a stem cell conditional culture medium filtering device which comprises a filtering box used for filtering a stem cell conditional culture medium, a cover seat is arranged on the periphery of the top of the filtering box, a feeding opening is fixedly formed in the top of the cover seat, and a connecting transverse pipe is fixedly connected to the bottom of the feeding opening. A plurality of sets of discharging openings are sequentially formed in the bottom of the connecting transverse pipe, a discharging pipe is installed below one side of the filtering box, and a water pump is installed at the top of the discharging pipe. According to the stem cell conditioned medium filtering device, large cell growth metabolism waste, dead cells, cell debris and other harmful substances in the stem cell conditioned medium are coarsely filtered, so that the filtering effect of the stem cell conditioned medium is improved, and the stem cell conditioned medium is directly filtered for the second time through the fine filtering assembly after being coarsely filtered; therefore, the filtering quality of the stem cell conditioned culture medium is improved, and unnecessary loss is reduced.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to a stem cell conditioned medium filtering device. Background Art

[0002] Stem cell conditioned medium is a medium used to support the growth and self-renewal of stem cells. It contains essential nutrients, growth factors and regulatory factors to provide environmental conditions suitable for stem cell growth and differentiation. Stem cells are a type of multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into a variety of functional cells. According to the developmental stage of stem cells, they are divided into embryonic stem cells and adult stem cells. In addition to beneficial components such as cytokines, stem cell conditioned medium also contains harmful substances such as waste products of cell growth metabolism, dead cells and cell fragments, which need to be filtered before use.

[0003] However, the stem cell conditioned medium filtration device of the prior art can only filter the stem cell conditioned medium once, thereby reducing the quality of the stem cell medium filtration and causing losses, and it is inconvenient to clean the inside of the filtration device; therefore, it does not meet the existing needs. In this regard, we propose a stem cell conditioned medium filtration device. Utility Model Content

[0004] The utility model provides a stem cell conditioned medium filtering device, which is equipped with a coarse filtration component, so as to facilitate the filtration of the stem cell conditioned medium, so as to perform coarse filtration on harmful substances such as large cell growth metabolic waste, dead cells and cell fragments contained therein, so as to improve the beneficial effect of stem cell conditioned medium filtration, and solve the problem mentioned in the above background technology that when filtering the stem cell conditioned medium, it can only be filtered once, thereby reducing the filtration quality of the stem cell medium.

[0005] The utility model provides the following technical solutions: a stem cell conditioned medium filtering device, comprising a filter box, a cover seat is provided on the top of the filter box, a feed port is fixedly installed on the top of the cover seat, and a connecting transverse pipe is fixedly connected to the bottom of the feed port, a plurality of groups of feed ports are sequentially installed on the bottom of the connecting transverse pipe, a discharge pipe is installed below one side of the filter box, and a water pump is installed on the top of the discharge pipe, two groups of coarse filter components for coarse filtering of stem cell conditioned medium are provided above the filter box, and a fine filter component is provided below the coarse filter component.

[0006] As an optional scheme of a stem cell conditioned culture medium filtration device of the utility model, wherein: the coarse filtration component includes a first slide seat, a first mounting plate, a first handle, a first positioning groove seat, a coarse filter plate and a coarse filter screen, the first mounting plate is fixedly mounted on the front of the first slide seat, and the first handle for hand holding is fixedly mounted on the surface of the first mounting plate, a through groove is provided inside the first slide seat, and first positioning groove seats for positioning and placing the coarse filter plate are installed on both sides of the through groove, the surface of the first positioning groove seat is provided with a coarse filter plate, and the surface of the coarse filter plate is provided with a coarse filter screen for primary filtration of the stem cell culture medium.

[0007] As an optional solution of a stem cell conditioned medium filtering device of the utility model, wherein: first card slots are opened at both ends of the surface of the first positioning slot, and a first card block is carded inside the first card slot, and a coarse filter plate is fixedly connected to the top of the first card block.

[0008] As an optional solution of a stem cell conditioned medium filtration device of the utility model, wherein: two groups of first openings are provided on the top of the filter box, and slide grooves are provided on both sides of the first openings, and the first slide seat is slidably connected inside the slide groove.

[0009] As an optional scheme of a stem cell conditioned medium filtering device of the utility model, wherein: the fine filtration component includes a second slide seat, a second mounting plate, a second handle, a second positioning slot seat, a fine filter plate and a fine filter net, the second mounting plate is fixedly mounted on the front side of the second slide seat, and the second handle is mounted on the surface of the second mounting plate, the second slide seat is provided with a through slot inside, and second positioning slot seats are mounted at both ends of the through slot, the second positioning slot seat is provided with a fine filter plate inside, and the surface of the fine filter net is provided with a fine filter net.

[0010] As an optional solution of a stem cell conditioned medium filtering device of the utility model, wherein: second card slots are opened at both ends of the surface of the second positioning slot, and a second card block is clamped inside the second card slot, and a fine filter plate is fixedly connected to the top of the second card block.

[0011] As an optional solution of a stem cell conditioned medium filtration device of the utility model, wherein: a second opening is opened at the center of the front of the filter box, slide grooves are opened on both sides of the second opening, and the interior of the slide groove is sealed and slidably connected to a second slide seat.

[0012] As an optional solution of a stem cell conditioned medium filtration device of the utility model, wherein: a sealing groove is opened on the top of the filter box, and a sealing card plate is sealed inside the sealing groove, and a cover seat is fixedly connected to the top of the sealing card plate.

[0013] The utility model has the following beneficial effects:

[0014] 1. The stem cell conditioned medium filtering device is provided with a coarse filtration component, thereby facilitating the filtration of the stem cell conditioned medium, so that harmful substances such as large cell growth metabolic waste, dead cells and cell fragments contained therein are subjected to coarse filtration, so as to improve the filtration effect of the stem cell conditioned medium, and through the cooperation of the first positioning slot, the first card slot and the first card block and other components, it is convenient to disassemble, clean and replace the coarse filter screen, so as to improve the effect of subsequent filtration.

[0015] 2. The stem cell conditioned medium filtering device is provided with a fine filtration component, so that after the stem cell conditioned medium is roughly filtered, it can be directly filtered for the second time through the fine filtration component, so as to improve the quality of the stem cell conditioned medium during filtration and reduce unnecessary losses. In addition, through the cooperation of the second positioning groove seat, the second card slot and the second card block and other components, it is convenient to disassemble, clean and replace the fine filter plate and the fine filter net.

[0016] 3. The stem cell conditioned medium filtering device is provided with a sealing groove, a sealing card plate and a cover seat, so as to seal the filter box and open it conveniently for cleaning the inside, so as to improve the effect of subsequent use and reduce pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0018] Figure 2 It is a schematic diagram of the structure of each component of the utility model.

[0019] Figure 3 It is a schematic diagram of the cover seat structure of the utility model.

[0020] Figure 4 It is a schematic diagram of the coarse filter structure of the utility model.

[0021] Figure 5 This is a schematic diagram of the fine filter structure of the utility model.

[0022] In the figure: 1. filter box; 2. sealing groove; 3. sealing card plate; 4. cover seat; 5. feed port; 6. connecting cross pipe; 7. discharge port; 8. first opening; 9. second opening; 10. slide groove; 11. first slide seat; 12. first mounting plate; 13. first handle; 14. first positioning groove seat; 15. first card slot; 16. first card block; 17. coarse filter plate; 18. coarse filter screen; 19. second slide seat; 20. second mounting plate; 21. second handle; 22. second positioning groove seat; 23. second card slot; 24. second card block; 25. fine filter plate; 26. fine filter screen; 27. discharge pipe; 28. water pump. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] Embodiment 1

[0025] This embodiment is intended to promote the solution of the problem that the existing stem cell conditioned medium filtering device can only filter the stem cell conditioned medium once, thereby reducing the quality of stem cell medium filtration, causing losses, and it is inconvenient to clean the inside of the filtering device. Please refer to Figure 1-5 A stem cell conditioned medium filtering device comprises a filter box 1, a cover seat 4 is provided on the top of the filter box 1, a feed port 5 is fixedly installed on the top of the cover seat 4, and a connecting transverse pipe 6 is fixedly connected to the bottom of the feed port 5, and a plurality of groups of feed ports 7 are sequentially installed at the bottom of the connecting transverse pipe 6, a discharge pipe 27 is installed at the bottom of one side of the filter box 1, and a water pump 28 is installed at the top of the discharge pipe 27. By setting the water pump 28, it is convenient to extract the filtered stem cell conditioned medium from the filter box 1, two groups of coarse filter components for coarse filtering of the stem cell conditioned medium are provided above the filter box 1, and a fine filter component is provided below the coarse filter component.

[0026] In this embodiment: the coarse filter assembly includes a first slide 11, a first mounting plate 12, a first handle 13, a first positioning groove seat 14, a coarse filter plate 17 and a coarse filter screen 18. The first mounting plate 12 is fixedly mounted on the front of the first slide 11, and the first handle 13 for hand holding is fixedly mounted on the surface of the first mounting plate 12. A through groove is provided inside the first slide 11, and first positioning groove seats 14 for positioning and placing the coarse filter plate 17 are installed on both sides of the through groove. The coarse filter plate 17 is provided on the surface of the first positioning groove seat 14, and the coarse filter screen 18 for primary filtration of the stem cell culture medium is provided on the surface of the coarse filter plate 17.

[0027] In this embodiment: first slots 15 are provided at both ends of the surface of the first positioning slot 14, and a first block 16 is clamped inside the first slot 15, and a coarse filter plate 17 is fixedly connected to the top of the first block 16. The design of these structures facilitates the filtration of the stem cell conditioned medium, so that harmful substances such as large cell growth metabolic waste, dead cells and cell fragments contained therein are coarsely filtered to improve the filtration effect of the stem cell conditioned medium, and the first positioning slot 14, the first slot 15 and the first block 16 cooperate to facilitate the disassembly, cleaning and replacement of the coarse filter screen 18 to improve the effect of subsequent filtration.

[0028] In this embodiment, two groups of first openings 8 are provided on the top of the filter box 1 , and slide grooves 10 are provided on both sides of the first openings 8 , and the first slide seats 11 are slidably connected inside the slide grooves 10 .

[0029] In this embodiment: the fine filter assembly includes a second slide 19, a second mounting plate 20, a second handle 21, a second positioning groove seat 22, a fine filter plate 25 and a fine filter screen 26. The second mounting plate 20 is fixedly mounted on the front of the second slide 19, and the second handle 21 is mounted on the surface of the second mounting plate 20. A through groove is opened inside the second slide 19, and second positioning groove seats 22 are mounted at both ends of the through groove. A fine filter plate 25 is arranged inside the second positioning groove seat 22, and a fine filter screen 26 is arranged on the surface of the fine filter plate 25.

[0030] In this embodiment, the second positioning slot seat 22 has second slots 23 at both ends thereof, and the second slots 23 are internally clamped with second clamping blocks 24 , and the top of the second clamping block 24 is fixedly connected with a fine filter plate 25 .

[0031] In this embodiment, a second opening 9 is provided at the center of the front side of the filter box 1 , sliding grooves 10 are provided on both sides of the second opening 9 , and a second sliding seat 19 is sealingly and slidably connected inside the sliding groove 10 .

[0032] In the present embodiment: a sealing groove 2 is provided on the top of the filter box 1, and a sealing card plate 3 is sealed inside the sealing groove 2, and a cover seat 4 is fixedly connected to the top of the sealing card plate 3. Through the design of these structures, the filter box 1 can be sealed, and it is also convenient to open it for cleaning the inside, so as to improve the effect of subsequent use and reduce pollution, and facilitate the stem cell conditioned medium to be directly filtered for the second time through the fine filtration component after the coarse filtration, so as to improve the quality of the stem cell conditioned medium during filtration and reduce unnecessary losses. In addition, through the cooperation of the second positioning groove seat 22, the second card slot 23, the second card block 24 and other components, it is convenient to disassemble, clean and replace the fine filter plate 25 and the fine filter net 26.

[0033] Working principle: put the stem cell conditioned medium to be filtered into the feed port 5, and then put it into the filter box 1 from the several groups of discharge ports 7 on the bottom of the connecting transverse tube 6 in turn, and then hold the first handle 13 by hand to drive the first slide 11 to slide from the first opening 8, and then make the first slide 11 slide through the slide groove 10 to contact the inner wall of the filter box 1, so that the coarse filter plate 17 on the first slide 11 and the coarse filter screen 18 are aligned with the discharge port, and filter once through the coarse filter screen 18 on the coarse filter plate 17, so that larger cell growth Harmful substances such as metabolic waste, dead cells and cell fragments are coarsely filtered. Otherwise, the first slide seat 11 can be pulled out, and then the coarse filter plate 17 can be hooked by hand to disengage the first card block 16 on the bottom from the first card slot 15, so as to facilitate its cleaning and replacement. After the coarse filtration is completed, the stem cell conditioned medium will be filtered twice through the fine filter net 26 on the fine filter plate 25, so as to improve the quality of stem cell conditioned culture filtration. After the filtration is completed, it falls to the bottom of the filter box 1. The operation process of the fine filtration component is consistent with that of the coarse filtration component.

[0034] After the fine filtration is completed, the water pump 28 is started to extract the stem cell conditioned medium at the bottom of the filter box 1 through the straw, and then discharged through the discharge pipe 27 into an external storage container for storage.

[0035] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0036] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A stem cell conditioned medium filtration device, comprising a filtration box (1), characterized in that: The top of the filter box (1) is provided with a cover seat (4), the top of the cover seat (4) is fixedly provided with a feed port (5), and the bottom of the feed port (5) is fixedly connected with a connecting transverse pipe (6), and the bottom of the connecting transverse pipe (6) is sequentially provided with a plurality of groups of feed ports (7), a discharge pipe (27) is installed below one side of the filter box (1), and a water pump (28) is installed on the top of the discharge pipe (27), and two groups of coarse filtration components for coarse filtration of stem cell conditioned medium are provided above the filter box (1), and a fine filtration component is provided below the coarse filtration component.

2. A stem cell conditioned medium filtration device according to claim 1, characterized in that: The coarse filter assembly comprises a first slide seat (11), a first mounting plate (12), a first handle (13), a first positioning groove seat (14), a coarse filter plate (17) and a coarse filter screen (18); the first mounting plate (12) is fixedly mounted on the front of the first slide seat (11), and the first handle (13) for holding by hand is fixedly mounted on the surface of the first mounting plate (12); a through groove is provided inside the first slide seat (11), and first positioning groove seats (14) for positioning and placing the coarse filter plate (17) are installed on both sides of the through groove; the coarse filter plate (17) is provided on the surface of the first positioning groove seat (14), and the coarse filter screen (18) for primary filtering of stem cell culture medium is provided on the surface of the coarse filter plate (17).

3. A stem cell conditioned medium filtration device according to claim 2, characterized in that: The first positioning slot seat (14) has first clamping slots (15) at both ends of its surface, and a first clamping block (16) is clamped inside the first clamping slot (15), and a coarse filter plate (17) is fixedly connected to the top of the first clamping block (16).

4. The stem cell conditioned medium filtration device according to claim 1, characterized in that: Two groups of first openings (8) are provided above the filter box (1), and sliding grooves (10) are provided on both sides of the first openings (8), and a first sliding seat (11) is slidably connected inside the sliding grooves (10).

5. The stem cell conditioned medium filtration device according to claim 1, characterized in that: The fine filter assembly comprises a second slide seat (19), a second mounting plate (20), a second handle (21), a second positioning groove seat (22), a fine filter plate (25) and a fine filter net (26); the second slide seat (19) is fixedly mounted with the second mounting plate (20) on the front side, and the second handle (21) is mounted on the surface of the second mounting plate (20); a through groove is provided inside the second slide seat (19), and second positioning groove seats (22) are mounted at both ends inside the through groove; a fine filter plate (25) is provided inside the second positioning groove seat (22), and a fine filter net (26) is provided on the surface of the fine filter plate (25).

6. The stem cell conditioned medium filtration device according to claim 5, characterized in that: Second clamping grooves (23) are provided at both ends of the surface of the second positioning groove seat (22), and a second clamping block (24) is clamped inside the second clamping groove (23), and a fine filter plate (25) is fixedly connected to the top of the second clamping block (24).

7. The stem cell conditioned medium filtration device according to claim 1, characterized in that: A second opening (9) is provided at the center of the front face of the filter box (1), sliding grooves (10) are provided on both sides of the second opening (9), and a second sliding seat (19) is sealingly slidably connected inside the sliding groove (10).

8. The stem cell conditioned medium filtration device according to claim 1, characterized in that: The top of the filter box (1) is provided with a sealing groove (2), and the interior of the sealing groove (2) is sealed and clamped with a sealing clamping plate (3), and the top of the sealing clamping plate (3) is fixedly connected with a cover seat (4).

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