Classification filtering device for protein purification

Abstract

The utility model discloses a graded filtering device for protein purification, which belongs to the technical field of protein purification treatment and comprises a cover plate, a pipeline joint communicated and connected with an opening of the cover plate and a filter cartridge corresponding to the cover plate, and a limit ring A and a limit ring B are fixedly connected inside the filter cartridge from top to bottom. A rough filtration membrane is inserted into an opening of the filter cartridge, a rubber ring A is adhered to the outer part of the rough filtration membrane, a transmission rod is inserted into the surface of the rough filtration membrane in a penetrating manner, a fine filtration membrane is fixedly connected to the bottom end of the transmission rod, and a rubber ring B is adhered to the outer part of the fine filtration membrane; the top end of the transmission rod is fixedly connected with a carrying table. After a liquid material is injected into the filter cartridge through the pipeline joint, the vibration motor is started through external control equipment, and the fine filter membrane and the rough filter membrane oscillate under the transmission action of the transmission rod, so that the phenomenon that impurities block filter holes of the fine filter membrane and the rough filter membrane is not easily caused, and the filter cartridge has very good filter performance.

Description

Technical Field

[0001] The utility model relates to the technical field of protein purification and treatment, and more specifically to a grading filtering device for protein purification. Background Art

[0002] The separation and purification of proteins is widely used in biochemical research applications and is an important operating technique. A typical eukaryotic cell can contain thousands of different proteins, some of which are very abundant and some of which contain only a few copies. In order to study a particular protein, it must first be purified from other proteins and non-protein molecules. There are many ways to purify proteins, and graded filtration is widely used.

[0003] After searching, the existing announcement number is CN217795010U, which is a protein purification and filtration device. The protein purification and filtration device is provided with a filter box; a blender is provided on the filter box; a feed funnel is provided on the blender; a rotating shaft is provided inside the blender; a connecting pipe is provided at the bottom of the blender; the connecting pipe connects the filter box and the blender; a centrifuge and a filter baffle are provided in the filter box, and the filter baffle is divided into a coarse filter baffle and a fine filter baffle. The protein can be thoroughly blended, and large pieces of protein are decomposed into small particles, which facilitates the purification and filtration of the protein; the protein can be filtered in layers and grades, the efficiency of protein separation and purification is improved, and the quality and purity of the protein after filtration and purification is guaranteed.

[0004] The commonly used graded filtration box actually has a coarse filtration membrane and a microfiltration membrane installed inside the filtration box in sequence. However, as the filtration operation proceeds, the filter residue is easily blocked on the surface of the filter membrane, affecting the filtration efficiency. Therefore, we proposed a graded filtration device for protein purification to solve the above problems. Utility Model Content

[0005] In view of the problems existing in the prior art, the purpose of the utility model is to provide a graded filtration device for protein purification, wherein a limit ring A and a limit ring B are installed inside the filter cartridge, the coarse filter membrane is fixedly connected to the fine filter membrane through a transmission rod, the rubber ring A outside the coarse filter membrane is bonded and fixed to the limit ring A, and the rubber ring B outside the fine filter membrane is bonded to the surface of the limit ring B. After the liquid material is injected into the interior of the filter cartridge through a pipe joint, the vibration motor is started through an external control device. Under the transmission action of the transmission rod, the fine filter membrane and the coarse filter membrane both oscillate, which is not easy to cause impurities to block the filter holes of the fine filter membrane and the coarse filter membrane, and has good filtering performance.

[0006] To solve the above problems, the utility model adopts the following technical solutions.

[0007] A graded filtration device for protein purification, comprising a cover plate, a pipe joint connected to an opening position of the cover plate, and a filter cartridge corresponding to the cover plate, wherein a limit ring A and a limit ring B are fixedly connected to the inside of the filter cartridge from top to bottom, a coarse filter membrane is inserted into the opening position of the filter cartridge, a rubber ring A is bonded to the outside of the coarse filter membrane, a transmission rod is inserted through the surface of the coarse filter membrane, a fine filter membrane is fixedly connected to the bottom end of the transmission rod, and a rubber ring B is bonded to the outside of the fine filter membrane;

[0008] The top end of the transmission rod is fixedly connected with a carrier, and the upper surface of the carrier is fixedly connected with a vibration motor.

[0009] Preferably, the coarse filtration membrane passes through the limiting ring A, and the rubber ring A and the limiting ring A are bonded and fixed.

[0010] Preferably, the fine filter membrane passes through the limiting ring B, and the rubber ring B is bonded to the surface of the limiting ring B.

[0011] Preferably, a reinforcement ring A is fixedly connected to the connection between the transmission rod and the coarse filter membrane, and a reinforcement ring B is fixedly connected to the connection between the transmission rod and the fine filter membrane.

[0012] Preferably, a sealing ring is filled at the connection between the cover plate and the filter cartridge, and a traction frame is fixedly connected to the outer wall of the cover plate.

[0013] Preferably, connecting rods are symmetrically mounted on the outer wall of the carrier, and the bottom ends of the connecting rods are fixedly connected to rubber seats.

[0014] Preferably, a support seat is symmetrically mounted on the inner wall of the filter cartridge, and the support seat is fixedly connected to the rubber seat.

[0015] Compared with the prior art, the advantages of the present invention are:

[0016] (1) In this scheme, a limit ring A and a limit ring B are installed inside the filter cartridge. The coarse filter membrane is fixedly connected to the fine filter membrane through a transmission rod. The rubber ring A on the outside of the coarse filter membrane is bonded to the limit ring A, and the rubber ring B on the outside of the fine filter membrane is bonded to the surface of the limit ring B. After the liquid material is injected into the interior of the filter cartridge through the pipe joint, the vibration motor is started through the external control device. Under the transmission action of the transmission rod, the fine filter membrane and the coarse filter membrane both vibrate, which is not easy to cause impurities to block the filter holes of the fine filter membrane and the coarse filter membrane, and has good filtering performance.

[0017] (2) In this scheme, connecting rods are symmetrically installed on the outer wall of the carrier, and the bottom ends of the connecting rods are fixedly connected to rubber seats. Support seats are symmetrically installed on the inner wall of the filter cylinder. The support seats and the rubber seats are fixedly connected. The connecting rods provide auxiliary support for the carrier. A rubber seat is bonded between the connecting rods and the support seats. The rubber seat does not affect the normal operation of the vibration motor. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0019] Figure 2 This is a schematic diagram of the filter cartridge and vibration motor structure of the utility model;

[0020] Figure 3 This is a schematic diagram of the internal structure of the filter cartridge of the utility model;

[0021] Figure 4 This is a schematic diagram of the structure of the coarse filter membrane and the fine filter membrane of the utility model;

[0022] Figure 5 This is a schematic structural diagram of the coarse filter membrane and the fine filter membrane of the utility model from another perspective.

[0023] Description of the numbers in the figure:

[0024] 1. Cover plate; 2. Pipe joint; 3. Filter cartridge; 4. Vibration motor; 5. Carrier; 6. Transmission rod; 7. Coarse filter membrane; 8. Connecting rod; 9. Rubber seat; 10. Support seat; 11. Limiting ring A; 12. Limiting ring B; 13. Fine filter membrane; 14. Rubber ring A; 15. Rubber ring B. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model; it is obvious that the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments, and all other embodiments obtained by ordinary technicians in this field based on the embodiments of the utility model without making creative work are within the scope of protection of the utility model. Example 1

[0026] See also Figure 1-5 A graded filtration device for protein purification, comprising a cover plate 1, a pipe joint 2 connected to the opening position of the cover plate 1, and a filter cartridge 3 corresponding to the cover plate 1, the interior of the filter cartridge 3 is fixedly connected with a limit ring A11 and a limit ring B12 from top to bottom, a coarse filter membrane 7 is inserted into the opening position of the filter cartridge 3, a rubber ring A14 is bonded to the outside of the coarse filter membrane 7, a transmission rod 6 is inserted through the surface of the coarse filter membrane 7, a fine filter membrane 13 is fixedly connected to the bottom end of the transmission rod 6, and a rubber ring B15 is bonded to the outside of the fine filter membrane 13;

[0027] The top end of the transmission rod 6 is fixedly connected to a carrier 5 , and the upper surface of the carrier 5 is fixedly connected to a vibration motor 4 .

[0028] The coarse filter membrane 7 passes through the limiting ring A11, the rubber ring A14 is bonded and fixed to the limiting ring A11, the fine filter membrane 13 passes through the limiting ring B12, the rubber ring B15 is bonded to the surface of the limiting ring B12, the connection between the transmission rod 6 and the coarse filter membrane 7 is fixedly connected with the reinforcement ring A, and the connection between the transmission rod 6 and the fine filter membrane 13 is fixedly connected with the reinforcement ring B.

[0029] It should be noted that a limit ring A11 and a limit ring B12 are installed inside the filter cartridge 3, the coarse filter membrane 7 is fixedly connected to the fine filter membrane 13 through the transmission rod 6, the rubber ring A14 on the outside of the coarse filter membrane 7 is bonded and fixed to the limit ring A11, and the rubber ring B15 on the outside of the fine filter membrane 13 is bonded to the surface of the limit ring B12. After the liquid material is injected into the interior of the filter cartridge 3 through the pipe joint 2, the vibration motor 4 is started through the external control device. Under the transmission action of the transmission rod 6, the fine filter membrane 13 and the coarse filter membrane 7 both oscillate, and it is not easy to cause impurities to block the filter holes of the fine filter membrane 13 and the coarse filter membrane 7, and it has good filtering performance.

[0030] See also Figure 1-4 The connection between the cover plate 1 and the filter cartridge 3 is filled with a sealing ring, the outer wall of the cover plate 1 is fixedly connected to a traction frame, the outer wall of the carrier 5 is symmetrically installed with connecting rods 8, the bottom end of the connecting rods 8 is fixedly connected to a rubber seat 9, the inner wall of the filter cartridge 3 is symmetrically installed with a support seat 10, and the support seat 10 and the rubber seat 9 are fixedly connected.

[0031] It should be noted that the outer wall of the carrier 5 is symmetrically installed with connecting rods 8, the bottom end of the connecting rods 8 is fixedly connected with a rubber seat 9, the inner wall of the filter cylinder 3 is symmetrically installed with a support seat 10, the support seat 10 and the rubber seat 9 are fixedly connected, the connecting rod 8 provides auxiliary support to the carrier 5, and the rubber seat 9 is bonded between the connecting rod 8 and the support seat 10, and the rubber seat 9 does not affect the normal operation of the vibration motor 4.

[0032] When in use: the inner part of the filter cartridge 3 is installed with a limit ring A11 and a limit ring B12, the coarse filter membrane 7 is fixedly connected with the fine filter membrane 13 through the transmission rod 6, the rubber ring A14 outside the coarse filter membrane 7 is bonded and fixed with the limit ring A11, and the rubber ring B15 outside the fine filter membrane 13 is bonded to the surface of the limit ring B12. After the liquid material is injected into the inner part of the filter cartridge 3 through the pipeline joint 2, the vibration motor 4 is started by the external control device. Under the transmission action of the transmission rod 6, the fine filter membrane 13 and the coarse filter membrane 7 are both vibrated, which is not easy to cause the phenomenon of impurities blocking the filter holes of the fine filter membrane 13 and the coarse filter membrane 7, and has good filtering performance; the outer wall of the carrier 5 is symmetrically installed with a connecting rod 8, and the bottom end of the connecting rod 8 is fixedly connected with a rubber seat 9, and the inner wall of the filter cartridge 3 is symmetrically installed with a support seat 10, and the support seat 10 and the rubber seat 9 are fixedly connected. The connecting rod 8 assists in supporting the carrier 5, and a rubber seat 9 is bonded between the connecting rod 8 and the support seat 10, and the rubber seat 9 does not affect the normal operation of the vibration motor 4.

[0033] The above is only a preferred specific implementation of the utility model; however, the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solution and improved ideas of the utility model within the technical scope disclosed by the utility model, which should be included in the protection scope of the utility model.

Claims

1. A protein purification grading filtration device, comprising a cover plate (1), a pipe joint (2) connected to an opening of the cover plate (1), and a filter cartridge (3) corresponding to the cover plate (1), characterized in that: The interior of the filter cartridge (3) is fixedly connected with a limit ring A (11) and a limit ring B (12) from top to bottom; a coarse filter membrane (7) is inserted into the opening of the filter cartridge (3); a rubber ring A (14) is bonded to the outside of the coarse filter membrane (7); a transmission rod (6) is inserted through the surface of the coarse filter membrane (7); a fine filter membrane (13) is fixedly connected to the bottom end of the transmission rod (6); and a rubber ring B (15) is bonded to the outside of the fine filter membrane (13); The top end of the transmission rod (6) is fixedly connected to a carrier (5), and the upper surface of the carrier (5) is fixedly connected to a vibration motor (4).

2. A protein purification grading filtration device according to claim 1, characterized in that: The coarse filtration membrane (7) passes through the limiting ring A (11), and the rubber ring A (14) and the limiting ring A (11) are bonded and fixed.

3. A protein purification grading filtration device according to claim 1, characterized in that: The fine filter membrane (13) passes through the limiting ring B (12), and the rubber ring B (15) is bonded to the surface of the limiting ring B (12).

4. A protein purification grading filtration device according to claim 1, characterized in that: A reinforcement ring A is fixedly connected to the connection between the transmission rod (6) and the coarse filter membrane (7), and a reinforcement ring B is fixedly connected to the connection between the transmission rod (6) and the fine filter membrane (13).

5. A protein purification grading filtration device according to claim 1, characterized in that: A sealing ring is filled at the connection between the cover plate (1) and the filter cartridge (3), and a traction frame is fixedly connected to the outer wall of the cover plate (1).

6. A protein purification grading filtration device according to claim 1, characterized in that: Connecting rods (8) are symmetrically mounted on the outer wall of the carrier (5), and the bottom ends of the connecting rods (8) are fixedly connected to rubber seats (9).

7. A protein purification grading filtration device according to claim 1, characterized in that: A support seat (10) is symmetrically mounted on the inner wall of the filter cartridge (3), and the support seat (10) and the rubber seat (9) are fixedly connected.

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