High-pressure homogenizing equipment

Abstract

The utility model discloses high-pressure homogenizing equipment which comprises a high-pressure homogenizer, a feeding device and a cooling device, the feeding device comprises a feeding hopper, a premixing and filtering assembly is arranged in the feeding hopper, a three-way valve is arranged at a discharging opening in the bottom of the feeding hopper, and a feeding opening of the high-pressure homogenizer is connected with the three-way valve through a hose; the cooling device comprises a low-temperature constant-temperature tank and a booster pump, a plurality of heat exchange plates are arranged in a water tank of the low-temperature constant-temperature tank, liquid inlets of the heat exchange plates are connected with a discharge port of the high-pressure homogenizer through a multi-way pipe, liquid outlets of the heat exchange plates are connected with the booster pump through a multi-way pipe, and the booster pump is connected with a three-way valve through a hose; according to the utility model, the premixing and filtering assembly in the feed hopper is used for filtering overlarge particle materials in samples and pre-mixing the samples, the low-temperature thermostatic bath can quickly cool the samples treated by the high-pressure homogenizer, and then the samples are sent back to the high-pressure homogenizer through the pressure pump, so that the samples are uniformly mixed. And the homogenizing effect of the sample is greatly improved after circular treatment.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedical test equipment, in particular to a high-pressure homogenizing device. Background Art

[0002] High-pressure homogenizers can homogenize and disperse samples in a suspended state by subjecting them to ultra-high pressure through high-speed impact, pressure release and shearing through a slit-type homogenizing valve. In the biomedicine field, high-pressure homogenizers are often used in processes such as cell disruption, nucleic acid extraction and protein purification. They help to dissociate cell structures, release organelles and separate biomacromolecules, and can more effectively obtain and purify the required biomolecules, providing a basis for subsequent research and drug development.

[0003] When using a high-pressure homogenizer, the sample is usually sent directly into the high-pressure homogenizer. However, the lack of pre-mixing of the sample is not conducive to improving the homogenization effect. In addition, large particles in the sample may clog the homogenization valve and affect the normal operation of the high-pressure homogenizer. At the same time, after passing through the high-pressure homogenizer, the sample will heat up rapidly due to high-speed impact, shearing and other effects. Excessive temperature will affect the stability of the sample, making it impossible for the sample to pass through the high-pressure homogenizer multiple times in a short period of time, making it difficult to improve the homogenization effect of the sample. Summary of the invention

[0004] The purpose of the utility model is to provide a high-pressure homogenizing device in view of the problems existing in the prior art.

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0006] A high-pressure homogenizing device comprises a high-pressure homogenizer, a feeding device and a cooling device, wherein the feeding device comprises a feeding hopper, wherein a premixing and filtering assembly is arranged in the feeding hopper, and a three-way valve is arranged at a discharge port at the bottom of the feeding hopper, and the feeding port of the high-pressure homogenizer is connected to the three-way valve through a hose; the cooling device comprises a low-temperature constant temperature bath and a pressure pump, wherein a plurality of heat exchange plates are arranged in a water tank of the low-temperature constant temperature bath, and the liquid inlet of the heat exchange plate is connected to the discharge port of the high-pressure homogenizer through a multi-way pipe, and the liquid outlet of the heat exchange plate is connected to the pressure pump through a multi-way pipe, and the pressure pump is connected to the three-way valve through a hose.

[0007] The utility model utilizes the premixing filter component in the feed hopper, which can filter out oversized particulate materials in the sample to avoid clogging the high-pressure homogenizer on the one hand, and on the other hand, premix the sample to improve the homogenization effect of the sample. The low-temperature constant temperature bath can quickly cool the sample processed by the high-pressure homogenizer, and then send the sample back to the high-pressure homogenizer through the pressure pump, which greatly improves the homogenization effect of the sample after the cyclic treatment.

[0008] Preferably, the premix filtration assembly includes a main shaft, a filter funnel and a stirring blade, the filter funnel and the stirring blade are both arranged on the main shaft, a limit sill and a support frame are provided in the feed hopper, the limit sill is arranged around the main shaft, the filter funnel is mounted on the limit sill, the limit sill is located above the support frame, and the bottom end of the main shaft is rotatably connected to the support frame.

[0009] The main shaft is used to drive the filter funnel and the stirring blade to rotate. The filter funnel and the stirring blade mix the sample together. At the same time, the filter funnel can filter out excessively large particulate materials in the sample.

[0010] Preferably, the feeding device also includes a motor and a gear reducer, the gear reducer includes a first gear and a second gear, the motor is connected to the first gear and the second gear through the first gear, the second gear is located between the limit sill and the support frame, and the second gear is connected to the main shaft.

[0011] The motor is connected to the main shaft through the gear reducer, so that the motor can be placed on one side of the feed hopper without affecting the discharge of materials from the bottom of the feed hopper.

[0012] Preferably, the main shaft includes a first rotating shaft and a second rotating shaft, a plug-in block is provided at the top end of the second rotating shaft, the bottom end of the first rotating shaft is plugged into the second rotating shaft through the plug-in block, the filter funnel is arranged on the first rotating shaft, and the second gear is connected to the second rotating shaft.

[0013] The first rotating shaft and the second rotating shaft are detachably connected together. When the filter funnel needs to be cleaned, the first rotating shaft can be pulled out to take out the filter funnel, so that the filter funnel can be cleaned quickly and conveniently.

[0014] Preferably, a one-way valve is provided on the three-way valve, the liquid inlet of the one-way valve is connected to the pressure pump through a hose, and the liquid outlet of the one-way valve is connected to the three-way valve.

[0015] The one-way valve can prevent the sample entering from the feeding hopper from entering the cooling device.

[0016] Preferably, the feeding device also includes a bracket, the feed hopper is arranged on the top plate of the bracket, the low-temperature constant temperature tank is located below the feed hopper, and the feed hopper is located at a height higher than the feed port of the high-pressure homogenizer, so that the sample in the feed hopper can flow into the high-pressure homogenizer by itself.

[0017] Compared with the prior art, the beneficial effects of the utility model are:

[0018] The utility model utilizes the premixing filter component in the feed hopper, which can filter out oversized particulate materials in the sample to avoid clogging the high-pressure homogenizer on the one hand, and on the other hand, premix the sample to improve the homogenization effect of the sample. The low-temperature constant temperature bath can quickly cool the sample processed by the high-pressure homogenizer, and then send the sample back to the high-pressure homogenizer through the pressure pump, which greatly improves the homogenization effect of the sample after the cyclic treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural schematic diagram of the utility model;

[0020] Figure 2 It is a schematic diagram of the structure of the feed hopper, motor and gear reducer;

[0021] Figure 3 It is a schematic diagram of the structure after the feed hopper is cut open and the first rotating shaft and the second rotating shaft are separated;

[0022] Figure 4 It is a schematic diagram of the structure of the water tank of the low temperature constant temperature tank;

[0023] In the figure: 1. high-pressure homogenizer; 2. feed hopper; 201. filter funnel; 202. stirring blade; 203. second gear; 204. first rotating shaft; 205. second rotating shaft; 206. support frame; 3. bracket; 4. low-temperature constant temperature bath; 401. first multi-way pipe; 402. heat exchange plate; 5. pressure pump; 6. motor; 7. gear reducer; 8. three-way valve; 801. one-way valve. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the utility model to clearly and completely describe the technical solution of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] In the description of the present invention, it should be noted that the directions or positional relationships indicated by terms such as "middle", "upper", "lower", "left", "right", "inside" and "outside" are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention.

[0026] like Figures 1 to 4As shown, the specific scheme of the embodiment is as follows: a high-pressure homogenization equipment includes a high-pressure homogenizer 1, a feeding device and a cooling device, the feeding device includes a feeding hopper 2, a bracket 3, a motor 6 and a gear reducer 7, and the cooling device includes a low-temperature constant temperature tank 4 and a pressure pump 5.

[0027] The feed hopper 2 passes through the housing of the gear reducer 7, and the feed hopper 2 is fixed together with the housing of the gear reducer 7. A mounting hole is provided on the top plate of the bracket 3, and the diameter of the mounting hole is the same as the maximum outer diameter of the feed hopper 2. The feed hopper 2 is arranged at the mounting hole, and the housing of the gear reducer 7 is pressed on the top plate of the bracket 3 to prevent the feed hopper 2 from falling off the top plate of the bracket 3.

[0028] A premix filter assembly, a limit sill and a support frame 206 are arranged in the feed hopper 2. The premix filter assembly includes a main shaft, a filter funnel 201 and a stirring blade 202. The limit sill and the support frame 206 are fixed on the inner wall of the feed hopper 2, and the limit sill is arranged around the main shaft.

[0029] The gear reducer 7 includes a first gear, a second gear 203 and a housing. The motor 6 is arranged on the housing of the gear reducer 7. The first gear and the second gear 203 are meshed. The output shaft of the motor 6 is connected to the first gear. The second gear 203 is sleeved on the main shaft. The motor 6 rotates the first gear to rotate the second gear 203, and then drives the main shaft to rotate through the second gear 203.

[0030] The main shaft includes a first rotating shaft 204 and a second rotating shaft 205 . A plug-in block is provided at the top of the second rotating shaft 205 , and the bottom end of the first rotating shaft 204 is plugged with the second rotating shaft 205 through the plug-in block.

[0031] A handle, a stirring blade 202, a filter funnel 201, a limit step, a second gear 203 and a support frame 206 are distributed from top to bottom in the feed hopper 2, wherein the handle is arranged at the top of the first rotating shaft 204, the stirring blade 202 and the filter funnel 201 are arranged on the first rotating shaft 204, and the second gear 203 and the support frame 206 are connected to the second rotating shaft 205.

[0032] The filter funnel 201 is mounted on a limiting step, which is used to limit the position of the filter funnel 201 . The bottom end of the second rotating shaft 205 is rotatably connected to the support frame 206 , and the support frame 206 is used to limit the position of the second rotating shaft 205 .

[0033] When the feed hopper 2 is used, the motor 6 is started to feed the sample solution directly into the feed hopper 2. The motor 6 drives the main shaft to rotate through the gear reducer 7. The handle, stirring blade 202 and filter funnel 201 on the main shaft will all rotate. The stirring blade 202 and the filter funnel 201 cooperate with each other to filter out larger particles in the sample solution while mixing the sample solution. The filtered sample solution passes through the second gear 203 and the support frame 206 and leaves the feed hopper 2 through the discharge port at the bottom of the feed hopper 2.

[0034] When cleaning the feed hopper 2 , the motor 6 is turned off, and the first rotating shaft 204 and the second rotating shaft 205 are separated by a handle to lift out the filter funnel 201 and the stirring blade 202 .

[0035] A three-way valve 8 is provided at the discharge port at the bottom of the feed hopper 2. The low-temperature constant temperature tank 4 is located below the feed hopper 2. The feed hopper 2 is located at a height higher than the feed port of the high-pressure homogenizer 1. The feed port of the high-pressure homogenizer 1 is connected to the three-way valve 8 through a hose.

[0036] A plurality of heat exchange plates 402 are arranged in the water tank of the low-temperature thermostatic tank 4. The low-temperature thermostatic tank 4 adopts compressor refrigeration. The low-temperature thermostatic tank 4 cools the cooling medium in the water tank through the refrigeration system, and then exchanges heat with the heat exchange plates 402 through the cooling medium, thereby reducing the temperature of the solution in the heat exchange plates 402. The cooling medium can be selected from water or ethylene glycol aqueous solution according to the cooling temperature.

[0037] A first multi-way pipe 401 and a second multi-way pipe are respectively provided on both sides of the low-temperature constant temperature tank 4. The liquid inlet of the heat exchange plate 402 is connected to the discharge port of the high-pressure homogenizer 1 through the first multi-way pipe 401, and the liquid outlet of the heat exchange plate 402 is connected to the pressure pump 5 through the second multi-way pipe. A one-way valve 801 is provided on the three-way valve 8. The pressure pump 5 is connected to the liquid inlet of the one-way valve 801 through a hose, and the liquid outlet of the one-way valve 801 is connected to the three-way valve 8.

[0038] The flow path of the sample solution in the high-pressure homogenizer 1, the feeding device and the cooling device is as follows: the sample solution enters the three-way valve 8 through the feeding hopper 2, and then enters the high-pressure homogenizer 1 through the feeding port of the high-pressure homogenizer 1. After homogenization, it enters the heat exchange plate 402 in the low-temperature constant temperature tank 4 through the discharge port of the high-pressure homogenizer 1 and the first multi-way pipe 401, exchanges heat with the cooling medium in the water tank when passing through the pipeline of the heat exchange plate 402, and then enters the booster pump 5 through the second multi-way pipe, and is pumped back to the three-way valve 8 by the booster pump 5, and then returns to the high-pressure homogenizer 1 through the three-way valve 8 for re-homogenization. After completing multiple homogenizations, remove the hose connecting the booster pump 5 to the one-way valve 801, and the booster pump 5 delivers the sample solution to the storage container through the hose.

[0039] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A high-pressure homogenization device, comprising a high-pressure homogenizer, a feeding device and a cooling device, characterized in that: The feeding device includes a feeding hopper, a premixing and filtering assembly is arranged in the feeding hopper, a three-way valve is arranged at the discharge port at the bottom of the feeding hopper, and the feeding port of the high-pressure homogenizer is connected to the three-way valve through a hose; the cooling device includes a low-temperature constant temperature tank and a pressure pump, a plurality of heat exchange plates are arranged in the water tank of the low-temperature constant temperature tank, the liquid inlet of the heat exchange plate is connected to the discharge port of the high-pressure homogenizer through a multi-way pipe, the liquid outlet of the heat exchange plate is connected to the pressure pump through a multi-way pipe, and the pressure pump is connected to the three-way valve through a hose.

2. A high pressure homogenization device according to claim 1, characterized in that: The premix filtration assembly includes a main shaft, a filter funnel and a stirring blade, the filter funnel and the stirring blade are both arranged on the main shaft, a limit sill and a support frame are arranged in the feed hopper, the limit sill is arranged around the main shaft, the filter funnel is mounted on the limit sill, the limit sill is located above the support frame, and the bottom end of the main shaft is rotatably connected to the support frame.

3. A high pressure homogenization device according to claim 2, characterized in that: The feeding device also includes a motor and a gear reducer, the gear reducer includes a first gear and a second gear, the motor is connected to the first gear and the second gear through the first gear, the second gear is located between the limit sill and the support frame, and the second gear is connected to the main shaft.

4. A high pressure homogenization device according to claim 3, characterized in that: The main shaft includes a first rotating shaft and a second rotating shaft. The top of the second rotating shaft is provided with a plug-in block. The bottom of the first rotating shaft is plugged with the second rotating shaft through the plug-in block. The filter funnel is arranged on the first rotating shaft. The second gear is connected to the second rotating shaft.

5. A high pressure homogenization device according to claim 1, characterized in that: The three-way valve is provided with a one-way valve, the liquid inlet of the one-way valve is connected to the pressure pump through a hose, and the liquid outlet of the one-way valve is connected to the three-way valve.

6. A high pressure homogenization device according to claim 1, characterized in that: The feeding device also includes a bracket, the feeding hopper is arranged on the top plate of the bracket, the low-temperature constant temperature tank is located below the feeding hopper, and the height of the feeding hopper is higher than the feeding port of the high-pressure homogenizer.

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