Constant-temperature oscillation device for batch resuscitation of cryopreserved cell samples

Abstract

The constant-temperature oscillation device comprises a shell, a water bath is fixedly installed in the shell, an opening is formed in the position, corresponding to the water bath, of the top of the shell, a controller is installed on the front wall of the shell, and the controller is connected with the water bath. An electric heater and a temperature sensor are fixedly mounted at the bottom of the water bath, a supporting plate is slidably mounted in the water bath, and a driving mechanism is mounted in the shell; by arranging the grating plate, the jackscrew, the nut, the rubber band and the like, a cryopreservation tube can be clamped in the insertion hole by using the rubber band only by operating the jackscrew on the top plate of the bracket to drive the grating plate to drive the rubber band to move, otherwise, the grating plate is operated to enable the rubber band to move to one side of the insertion hole, so that the rubber band is separated from the cryopreservation tube; the clamping and releasing device is simple and convenient to operate, clamping and releasing of all the cryopreservation tubes on the bracket can be achieved at a time, batch operation of the cryopreservation tubes is facilitated, and the use effect is good.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental equipment, in particular to a constant temperature oscillation device for batch recovery of frozen cell samples. Background Art

[0002] Cell recovery is a biological term that refers to the process of thawing cells frozen in liquid nitrogen or a -70°C freezer and then re-culturing them to resume cell growth.

[0003] During the cell recovery process, the cryovials need to be taken out of the freezing environment and placed in a constant temperature water bath shaker. The constant temperature water bath shaker heats the cryovials in a 37°C water bath and oscillates the cryovials during the heating process to allow the samples in the cryovials to be thawed evenly and quickly, thereby preventing the ice crystals from slowly melting and entering the cells to form recrystallization, which may damage the cells.

[0004] When in use, the constant temperature water bath shaker will drive the tube rack storing the cryotubes to shake so as to oscillate the samples in the cryotubes. In order to prevent the cryotubes from falling off the tube rack or colliding with each other, a clamping structure for positioning the cryotubes is usually installed on the tube rack. However, most of the current clamping structures are independent and can only clamp a single cryotube. Therefore, when frozen cell samples are revived in batches, a large number of clamping mechanisms need to be operated to clamp or release each cryotube. The process is relatively cumbersome and needs to be improved. Utility Model Content

[0005] The purpose of the utility model is to provide a constant temperature oscillation device for batch recovery of frozen cell samples to solve the problems raised in the above background technology.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] A constant temperature oscillation device for batch recovery of frozen cell samples comprises a shell, a water bath is fixedly installed in the shell, an opening is opened at the top of the shell corresponding to the water bath, a controller is installed on the front wall of the shell, an electric heater and a temperature sensor are fixedly installed at the bottom of the water bath, a support plate is slidably installed in the water bath, a driving mechanism is installed in the shell, the driving mechanism is used to drive the support plate to reciprocate in the water bath, the top of the support plate is detachably connected to a bracket, the bracket comprises a bottom plate, the top of the bottom plate is fixedly connected to a top plate through a plurality of vertical rods, a plurality of sockets are penetrated through the top of the top plate, the sockets are used to insert cryopreservation tubes, a grid plate is slidably connected to the top of the top plate, a rubber band is detachably connected to the top of the grid plate and the top of the grid plate and the corresponding position of each socket are connected, a nut is fixedly connected to the top of the top plate, and a top screw is cooperatively penetrated on the nut, and the top screw abuts against one end of the grid plate.

[0008] As a further solution of the utility model: the top of the top plate is located on both sides of the grid plate and is symmetrically fixedly connected with two L-shaped bars, and the grid plate is located on the inner sides of the two L-shaped bars.

[0009] As a further solution of the utility model: the top of the grid plate is located on both sides of each insertion hole and is fixedly connected with pin rods, the two ends of the rubber band are sleeved on the corresponding pin rods, and the top end of the pin rod is fixedly connected to a limiting block.

[0010] As a further solution of the utility model: the outer wall of the top plate is fixedly connected with a plurality of connecting ears, the connecting ears are penetrated with hand bolts, the top of the support plate and the corresponding parts of each connecting ear are fixedly connected with columns, the top of the columns are provided with threaded holes compatible with the hand bolts, and the hand bolts are matched and connected with the corresponding threaded holes.

[0011] As a further solution of the utility model: the driving mechanism includes a reduction motor fixedly installed in the shell, the output shaft of the reduction motor is fixedly connected to the turntable, the end surface of the turntable is rotatably connected to a connecting rod via a pin shaft, the end of the connecting rod away from the turntable is rotatably connected to a sliding rod, the outer wall of the water bath is fixedly connected to a sleeve, the end of the sliding rod away from the connecting rod is inserted into the water bath by the sleeve and then fixedly connected to the support plate, a sealing ring is embedded in the sleeve, and the sealing ring fits the outer wall of the sliding rod.

[0012] As a further solution of the utility model: the inner wall of the water bath below the support plate is fixedly connected with a plurality of guide rods, the outer walls of the guide rods are slidably sleeved with sliding sleeves, and the sliding sleeves are fixedly connected to the bottom of the support plate.

[0013] As a further solution of the utility model: the bottom of the water bath is fixedly connected with a drain pipe, and the water outlet end of the drain pipe passes through the shell and is installed with a valve.

[0014] As a further solution of the utility model: a cover is inserted into the opening, a plurality of support bars are fixedly connected to the inner wall of the water bath, and the bottom of the cover abuts against the top of the support bar.

[0015] Compared with the prior art, the beneficial effects of the utility model are:

[0016] The utility model provides a grid plate, a top screw, a nut and a rubber band, etc., and only needs to operate the top screw on the top plate of the bracket to drive the grid plate to drive the rubber band to move, so that the rubber band can be used to clamp the cryogenic tube in the insertion hole; conversely, the grid plate is operated to move the rubber band to one side of the insertion hole so that the rubber band is separated from the cryogenic tube and the cryogenic tube can be taken out of the insertion hole. The operation is simple and convenient, and the clamping and releasing of all the cryogenic tubes on the bracket can be realized at one time, which is convenient for batch operation of the cryogenic tubes and has a good use effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The schematic diagram is a structural diagram of a constant temperature oscillation device for batch recovery of frozen cell samples.

[0018] Figure 2 The figure is a schematic diagram of the internal structure of a shell in a constant temperature oscillation device for batch revival of frozen cell samples.

[0019] Figure 3 The diagram is a top view of the structure of a clamping mechanism in a constant temperature oscillation device for batch revival of frozen cell samples.

[0020] Figure 4 The present invention is a schematic diagram of the side cross-sectional structure of a bracket in a constant temperature oscillating device for batch revival of frozen cell samples.

[0021] Figure 5 The present invention is a structural schematic diagram of the cooperation between a clamping mechanism and a cryopreservation tube in a constant temperature oscillation device for batch revival of frozen cell samples.

[0022] Among them, the shell 1, the controller 2, the opening 3, the cover 4, the water bath 5, the support bar 6, the drain pipe 7, the valve 8, the electric heater 9, the temperature sensor 10, the guide rod 11, the sliding sleeve 12, the support plate 13, the sliding rod 14, the sleeve 15, the reduction motor 16, the turntable 17, the connecting rod 18, the column 19, the bracket 20, the bottom plate 21, the vertical rod 22, the top plate 23, the jack 24, the grille plate 25, the L-shaped bar 26, the pin 27, the limit block 28, the rubber band 29, the nut 30, the top screw 31, the connecting ear 32, the hand-tightened bolt 33, and the cryopreservation tube 34. DETAILED DESCRIPTION

[0023] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention will be further described in detail in conjunction with the embodiments below. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0024] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0025] It should be understood that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation in the specification, and therefore cannot be understood as a limitation on the present invention.

[0026] In addition, the terms "first", "second", and "third" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features.

[0027] See also Figures 1 to 5 In an embodiment of the utility model, a constant temperature oscillation device for batch recovery of frozen cell samples includes a shell 1, a water bath 5 is fixedly installed in the shell 1, an opening 3 is opened at the top of the shell 1 corresponding to the water bath 5, a controller 2 is installed on the front wall of the shell 1, an electric heater 9 and a temperature sensor 10 are fixedly installed at the bottom of the water bath 5, a support plate 13 is slidably installed in the water bath 5, a driving mechanism is installed in the shell 1, the driving mechanism is used to drive the support plate 13 to reciprocate in the water bath 5, and the top of the support plate 13 is detachable A bracket 20 is connected, and the bracket 20 includes a bottom plate 21. The top of the bottom plate 21 is fixedly connected to a top plate 23 through a plurality of vertical rods 22. A plurality of insertion holes 24 are penetrated through the top of the top plate 23. The insertion holes 24 are used to insert cryotubes 34. A grid plate 25 is slidably connected to the top of the top plate 23. A rubber band 29 is detachably connected to the top of the grid plate 25 and the corresponding position of each insertion hole 24. A nut 30 is fixedly connected to the top of the top plate 23, and a top screw 31 is inserted through the nut 30. The top screw 31 abuts against one end of the grid plate 25.

[0028] By adopting the above scheme, the utility model, when in use, inserts each cryotube 34 that needs to be resuscitated in batches into the corresponding insertion hole 24, rotates the top screw 31 to push the grid plate 25 to slide on the top plate 23, so that the two ends of the rubber band 29 move to the two sides of the corresponding insertion hole 24, at this time, the rubber band 29 will be stretched, and the middle part will be close to the corresponding cryotube 34, so that the cryotube 34 is clamped in the insertion hole 24 by the elastic force of the rubber band 29;

[0029] Then, the bracket 20 is mounted on the support plate 13 so that the bottom end of the cryotube 34 is inserted into the pre-heated warm water, and the driving mechanism is started to drive the support plate 13 to move back and forth in the water bath 5, so that the sample in the cryotube 34 can be thawed;

[0030] After the sample is thawed, the bracket 20 is taken out of the water bath 5, and then the top screw 31 is rotated to slide the grid plate 25 on the top plate 23, so that each rubber band 29 moves to the side of the corresponding socket 24, so that the rubber band 29 can be separated from the cryogenic tube 34, thereby facilitating the cryogenic tube 34 to be taken out of the socket 24, and the operation is simple and convenient.

[0031] Specific combination Figure 3-Figure 5 In one embodiment of the utility model, the top of the top plate 23 is located on both sides of the grille plate 25 and is symmetrically fixedly connected with two L-shaped bars 26, and the grille plate 25 is located on the inner side of the two L-shaped bars 26. Through the setting of the two L-shaped bars 26, the grille plate 25 can be positioned and guided on the top plate 23 to improve the stability of the grille plate 25 sliding on the top plate 23.

[0032] Specific combination Figure 3-Figure 5 In one embodiment of the utility model, the top of the grid plate 25 is located on both sides of each insertion hole 24 and is fixedly connected with a pin rod 27, both ends of the rubber band 29 are sleeved on the corresponding pin rod 27, and the top of the pin rod 27 is fixedly connected to a limiting block 28.

[0033] The provision of the pin rod 27 facilitates the disassembly and assembly of the rubber band 29 on the grid plate 25 , thereby facilitating rapid replacement of the rubber band 29 after it is damaged. The provision of the limit block 28 can effectively prevent the rubber band 29 from slipping off the top of the pin rod 27 .

[0034] Specific combination Figure 2-Figure 5In one embodiment of the utility model, the outer wall of the top plate 23 is fixedly connected with a plurality of connecting ears 32, and the connecting ears 32 are penetrated with hand bolts 33. The top of the support plate 13 and the corresponding parts of each connecting ear 32 are fixedly connected with a column 19, and the top of the column 19 is provided with a threaded hole adapted to the hand bolt 33. The hand bolt 33 is connected with the corresponding threaded hole. The cooperation between the hand bolt 33 and the threaded hole facilitates the disassembly and assembly of the bracket 20 on the support plate 13.

[0035] Specific combination Figure 2 In one embodiment of the utility model, the driving mechanism includes a reduction motor 16 fixedly installed in the housing 1, the output shaft of the reduction motor 16 is fixedly connected to a turntable 17, the end surface of the turntable 17 is rotatably connected to a connecting rod 18 through a pin shaft, the end of the connecting rod 18 away from the turntable 17 is rotatably connected to a slide rod 14, the outer wall of the water bath 5 is fixedly sealed and connected with a sleeve 15, the end of the slide rod 14 away from the connecting rod 18 is inserted into the water bath 5 by the sleeve 15 and is fixedly connected to the support plate 13, a sealing ring is embedded in the sleeve 15, and the sealing ring fits the outer wall of the slide rod 14.

[0036] By starting the reduction motor 16 to drive the turntable 17 to rotate, the turntable 17 can be used to drive one end of the connecting rod 18 to rotate around the center of the turntable 17, so that the connecting rod 18 can drive the slide bar 14 to perform reciprocating linear motion, thereby driving the support plate 13 to perform reciprocating linear motion in the water bath 5. The setting of the sleeve 15 and the sealing ring can ensure the sealing between the slide bar 14 and the water bath 5.

[0037] Specific combination Figure 2 In one embodiment of the utility model, the inner wall of the water bath 5 below the support plate 13 is fixedly connected with a plurality of guide rods 11 , the outer walls of the guide rods 11 are slidably sleeved with sliding sleeves 12 , and the sliding sleeves 12 are fixedly connected to the bottom of the support plate 13 .

[0038] Through the cooperation between the guide rod 11 and the sliding sleeve 12 , the support plate 13 can be guided in the water bath 5 , so as to improve the stability of the movement of the support plate 13 .

[0039] Specific combination Figure 2 In one embodiment of the utility model, a drain pipe 7 is fixedly connected to the bottom of the water bath 5, and a valve 8 is installed after the water outlet end of the drain pipe 7 passes through the shell 1. Through the arrangement of the drain pipe 7 and the valve 8, after the water bath heating of the freezing tube 34 is completed, the water in the water bath 5 can be conveniently discharged.

[0040] Specific combination Figure 2In one embodiment of the utility model, a cover 4 is provided in the opening 3 , a plurality of support bars 6 are fixedly connected to the inner wall of the water bath 5 , and the bottom of the cover 4 abuts against the top of the support bars 6 .

[0041] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art may still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A constant temperature oscillation device for batch thawing of frozen cell samples, characterized in that: The invention comprises a shell (1), a water bath (5) is fixedly installed in the shell (1), an opening (3) is provided at the top of the shell (1) corresponding to the water bath (5), a controller (2) is installed on the front wall of the shell (1), an electric heater (9) and a temperature sensor (10) are fixedly installed at the bottom of the water bath (5), a support plate (13) is slidably installed in the water bath (5), a driving mechanism is installed in the shell (1), the driving mechanism is used to drive the support plate (13) to reciprocate in the water bath (5), the top of the support plate (13) is detachably connected to a bracket (20), and the bracket (20) comprises a bottom The top of the bottom plate (21) is fixedly connected to a top plate (23) through a plurality of vertical rods (22); a plurality of insertion holes (24) are provided through the top of the top plate (23); the insertion holes (24) are used to insert freezing tubes (34); a grid plate (25) is slidably connected to the top of the top plate (23); a rubber band (29) is detachably connected to the top of the grid plate (25) and the corresponding position of each insertion hole (24); a nut (30) is fixedly connected to the top of the top plate (23); and a top screw (31) is inserted through the nut (30); the top screw (31) abuts against one end of the grid plate (25).

2. A constant temperature oscillation device for batch thawing of frozen cell samples according to claim 1, characterized in that: The top of the top plate (23) is located on both sides of the grid plate (25) and is symmetrically fixedly connected with two L-shaped bars (26), and the grid plate (25) is located on the inner sides of the two L-shaped bars (26).

3. A constant temperature oscillation device for batch thawing of frozen cell samples according to claim 1, characterized in that: The top of the grid plate (25) is located at both sides of each insertion hole (24) and is fixedly connected to a pin rod (27), both ends of the rubber band (29) are sleeved on the corresponding pin rod (27), and the top of the pin rod (27) is fixedly connected to a limit block (28).

4. A constant temperature oscillation device for batch thawing of frozen cell samples according to claim 1, characterized in that: The outer wall of the top plate (23) is fixedly connected with a plurality of connecting ears (32), and the connecting ears (32) are provided with hand-tightening bolts (33). The top of the support plate (13) and the corresponding positions of each connecting ear (32) are fixedly connected with a column (19), and the top of each column (19) is provided with a threaded hole matched with the hand-tightening bolt (33), and the hand-tightening bolt (33) is matched and connected with the corresponding threaded hole.

5. A constant temperature oscillation device for batch thawing of frozen cell samples according to claim 1, characterized in that: The driving mechanism comprises a reduction motor (16) fixedly mounted in a housing (1); an output shaft of the reduction motor (16) is fixedly connected to a turntable (17); a connecting rod (18) is rotatably connected to the end surface of the turntable (17) via a pin; an end of the connecting rod (18) away from the turntable (17) is rotatably connected to a slide bar (14); an outer wall of the water bath (5) is fixedly connected to a sleeve (15); an end of the slide bar (14) away from the connecting rod (18) is inserted into the water bath (5) via the sleeve (15) and is fixedly connected to the support plate (13); a sealing ring is embedded in the sleeve (15), and the sealing ring fits the outer wall of the slide bar (14).

6. A constant temperature oscillation device for batch thawing of frozen cell samples according to claim 1, characterized in that: The inner wall of the water bath (5) below the support plate (13) is fixedly connected to a plurality of guide rods (11), the outer walls of the guide rods (11) are slidably sleeved with sliding sleeves (12), and the sliding sleeves (12) are fixedly connected to the bottom of the support plate (13).

7. A constant temperature oscillation device for batch thawing of frozen cell samples according to claim 1, characterized in that: The bottom of the water bath (5) is fixedly connected to a drain pipe (7), and a valve (8) is installed after the water outlet end of the drain pipe (7) passes through the shell (1).

8. A constant temperature oscillation device for batch thawing of frozen cell samples according to claim 1, characterized in that: A cover (4) is inserted into the opening (3), and a plurality of support bars (6) are fixedly connected to the inner wall of the water bath (5), and the bottom of the cover (4) abuts against the top of the support bars (6).

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