Calprotectin detection kit

Abstract

The utility model relates to the technical field of biological detection, in particular to a calprotectin detection kit which comprises a detection shell and further comprises a sample adding tube, a base plate, a metering mechanism, an exchange mechanism and two pieces of detection test paper, the base plate is arranged in the detection shell in a sliding mode, and the two pieces of detection test paper are symmetrically arranged above the base plate. The sample adding pipe is mounted at the top end of the detection shell and located above one piece of detection test paper, through the metering mechanism, the amount of a sample dropped into a sample pad of the detection test paper can be metered, it is guaranteed that the amount of the dropped sample is within a specified range, and it is avoided that the detection precision is affected by too much or too little amount of the dropped sample; the two pieces of detection test paper are arranged, a sample can be detected twice at a time, the problem that when one-time operation is wrong, another test box needs to be additionally taken for retesting, and the test cost is increased can be avoided, and meanwhile the accuracy of a detection result is improved through two-time detection.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, in particular to a calprotectin detection kit. Background Art

[0002] Calprotectin refers to the calcium-binding protein content in the body's granulocytes. It can be used as a basis for examining acute inflammatory cell activation, and can also be used as a basis for cell-specific or tissue-specific examinations. Currently, people diagnose the patient's condition by testing calprotectin in feces.

[0003] The existing Chinese utility model patent with patent announcement number CN214310549U discloses an efficient fecal calprotectin detection kit, including a shell, a housing cavity is provided inside the shell, a slot is provided on one side of the shell, a connection port is provided on one side of the slot, and the connection port is connected with the housing cavity, and a dilution box is provided on one side of the shell. The sample is taken with a sampling stick and placed inside the dilution box, and the dilution liquid is placed inside the dilution box, the slot of the shell is plugged into the socket of the dilution box, the conical plug-in pierces the sealing tin foil, and the dilution box is tilted to make the sample flow to the colored particle / fluorescent particle conjugate pad and the nitrocellulose membrane, which can effectively avoid the problem of sample dripping out.

[0004] The above-mentioned calprotectin detection kit has the following deficiencies:

[0005] 1. When the dilution box is tilted to allow the sample to flow onto the colored particle / fluorescent particle conjugate pad and the nitrocellulose membrane, the amount of sample entering the shell cannot be controlled. When the sample amount is too much or too little, the detection accuracy will be affected;

[0006] 2. One test box can only perform one test. However, when an operation is incorrect, an additional test box needs to be taken out for retesting, which increases the test cost.

[0007] In view of the above problems, a calprotectin detection kit is now provided. Utility Model Content

[0008] The purpose of the utility model is to provide a calprotectin detection kit to solve the problems raised in the above background technology.

[0009] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0010] A calprotectin detection kit comprises a detection housing, a sample adding tube, a substrate, a metering mechanism, a switching mechanism and two detection test strips, wherein the substrate is slidably arranged inside the detection housing, the two detection test strips are symmetrically arranged above the substrate, the sample adding tube is installed at the top of the detection housing, and the sample adding tube is located above one of the detection test strips;

[0011] The metering mechanism includes a scale line, two baffles and a moving assembly for driving the two baffles to move, the scale line is located on one side of the sample tube, the two baffles are symmetrically plugged on both sides of the sample tube, and the baffles are slidably connected to the sample tube, and the moving assembly is located between the sample tube and the two baffles;

[0012] The switching mechanism includes a strip groove, a sliding rod, a pushing block and a positioning assembly for positioning the pushing block. The strip groove is opened at one end of the detection shell, the sliding rod is inserted into the inside of the strip groove and is slidably arranged in the strip groove, one end of the sliding rod is fixedly connected to the base plate, and the other end of the sliding rod is fixedly connected to the pushing block. The positioning assembly is located between the pushing block and the detection shell.

[0013] As a further solution of the utility model: the moving assembly includes two end plates, two mounting plates, two guide rods and four second springs;

[0014] The two end plates are respectively fixed on the sides of the two baffles away from each other, the two mounting plates are symmetrically fixed on both sides of the top of the detection shell, the two guide rods are symmetrically fixed between the two ends of the two mounting plates, one end of each end plate is sleeved and slidably set on a guide rod, each two second springs are sleeved on the surface of a guide rod, and the two ends of each second spring are respectively fixedly connected to the end plate and the mounting plate.

[0015] As a further solution of the utility model: one end of each end plate is fixedly connected to an inclined rod, the two inclined rods are staggered, and one end of each inclined rod away from the end plate is fixed with a toggle plate.

[0016] As a further solution of the utility model: an inclined scraper for guiding is fixed to the inner wall of the sample adding tube, and the bottom end surface of the inclined scraper is in contact with the upper surface of the baffle.

[0017] As a further solution of the utility model: a sealing strip for achieving a sealing effect is provided on one side of the two baffles close to each other.

[0018] As a further solution of the utility model: the positioning assembly includes two countersunk holes, two first springs, two balls and four positioning grooves matched with the balls;

[0019] Two countersunk holes are symmetrically opened on the upper and lower sides of the push block, and a support frame for installing the ball is slidably arranged inside each countersunk hole, and each ball is installed inside a support frame, and each first spring is fixed inside a countersunk hole, and the other end of each first spring is fixedly connected to the support frame;

[0020] The four positioning grooves are respectively located on both sides of the push block, and the four positioning grooves are symmetrically arranged on the detection housing, and the two balls are respectively inserted into the inside of two of the positioning grooves.

[0021] As a further solution of the utility model: two guide rails are fixedly installed inside the detection housing and on a side of the substrate away from the detection test paper, and the substrate is slidably arranged inside the detection housing via the guide rails;

[0022] As a further solution of the utility model: an observation window for easy observation is opened on the surface of the detection shell, and the observation window is located above one of the detection test strips.

[0023] As a further solution of the utility model: a handle convenient for holding is fixed to one end of the detection housing away from the pushing block.

[0024] As a further solution of the utility model: the surfaces of the handle and the push block are provided with anti-skid stripes for preventing skidding.

[0025] Compared with the prior art, the beneficial effects of the utility model are:

[0026] 1. The utility model provides a calprotectin detection kit, which can measure and control the amount of sample dripped onto the sample pad of the test paper by setting a metering mechanism, so as to ensure that the amount of sample dripped is within a specified range and avoid the influence of too much or too little sample dripped on the detection accuracy.

[0027] 2. A calprotectin detection kit of the utility model uses a baffle and a movable component. Before adding the sample and after heating the sample, the baffle can seal the sample tube to ensure the sealing effect inside the detection shell and prevent foreign matter from entering and affecting the detection accuracy. At the same time, it can also receive the sample dripped into the sample tube, which is beneficial to the subsequent measurement of the sample amount.

[0028] 3. The utility model provides a calprotectin detection kit, which, by providing a replacement mechanism, provides two detection test strips inside the detection housing, and can perform two tests on a sample at one time, which can not only avoid the problem of needing to take an additional test kit for retesting when an operation is wrong, thereby increasing the test cost, but also the two tests increase the accuracy of the test results;

[0029] 4. A calprotectin detection kit of the utility model is provided with an inclined scraper made of silicone material inside the sample tube, which not only plays a role in guiding the material, but also can scrape the surface of the baffle when the two baffles are away from each other, so as to avoid residual sample on the surface of the baffle, so that the sample can be more completely dropped on the sample pad on the test paper. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a structural schematic diagram of the utility model.

[0031] Figure 2 For this utility model Figure 1 Schematic diagram of the enlarged structure of part A.

[0032] Figure 3 It is a top view of the utility model.

[0033] Figure 4 It is a partial cross-sectional structural schematic diagram of the detection shell in the utility model.

[0034] Figure 5 For this utility model Figure 4 Schematic diagram of the enlarged structure of part B.

[0035] Figure 6 It is a structural schematic diagram of the base plate in the utility model.

[0036] Figure 7 It is a cross-sectional view of the pushing block in the utility model.

[0037] Among them: 11. detection shell; 12. detection test paper; 13. sample tube; 14. observation window; 15. handle; 16. baffle; 17. end plate; 18. mounting plate; 19. guide rod; 20. inclined rod; 21. toggle plate; 22. inclined scraper; 23. scale line; 24. base plate; 25. guide rail; 26. strip groove; 27. slide rod; 28. push block; 29. ​​positioning groove; 30. ball; 31. support frame; 32. countersunk hole; 33. first spring; 34. second spring. DETAILED DESCRIPTION

[0038] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0039] The utility model provides the following preferred embodiments:

[0040] like Figure 1-Figure 7As shown, a calprotectin detection kit includes a detection shell 11, a sample tube 13, a substrate 24, a metering mechanism, a switching mechanism and two detection test strips 12, the substrate 24 is slidably arranged inside the detection shell 11, and the two detection test strips 12 are symmetrically arranged above the substrate 24, and the substrate 24 is used to support and install the detection test strips 12 so as to drive the movement of the detection test strips 12 later, and the sample tube 13 is installed at the top of the detection shell 11, and the sample tube 13 is located above one of the detection test strips 12, specifically, the sample tube 13 is located above the sample pad in one of the detection test strips 12, that is, the sample can be dripped onto the sample pad in the detection test strip 12 through the sample tube 13, and it should be noted that the top surface of the detection test strip 12 is in contact with the inner wall of the detection shell 11;

[0041] The test paper 12 includes a sample pad, a colloidal gold labeling pad, a nitrocellulose membrane and a water-absorbing paper, and the sample pad, the colloidal gold labeling pad, the nitrocellulose membrane and the water-absorbing paper are overlapped in sequence. It should be noted that the test paper 12 is a prior art and will not be specifically described here;

[0042] The metering mechanism includes a scale line 23, two baffles 16 and a moving assembly for driving the two baffles 16 to move. The scale line 23 is located on one side of the sample tube 13. The two baffles 16 are symmetrically plugged on both sides of the sample tube 13, and the baffles 16 are slidably connected to the sample tube 13. Specifically, a sealing member is provided between the baffle 16 and the sample tube 13. Before adding the sample and after heating the sample, the baffle 16 can block the sample tube 13 to ensure the sealing effect inside the detection housing 11, prevent foreign matter from entering and affecting the detection accuracy, and at the same time The sample dripped into the sample tube 13 can also be received, which is conducive to the subsequent measurement of the sample amount. The moving component is located between the sample tube 13 and the two baffles 16. It should be noted that the sample tube 13 is made of a transparent material, which is convenient for comparing the sample liquid level with the scale on the scale line 23, and then convenient for knowing the amount of added sample, and can achieve the measurement and control of the amount of sample dripped into the sample pad of the test paper 12, ensuring that the amount of sample dripped is within the specified range, and avoiding too much or too little sample dripped to affect the detection accuracy;

[0043] In the initial state, the ends of the two baffles 16 that are close to each other abut against each other. When dripping the sample, the sample is first dripped onto the two baffles 16, and the baffles 16 receive the sample. The sample amount is observed using the scale line 23. When the amount of the dripped sample is within the specified range, the baffles 16 are moved using the moving assembly so that the two baffles 16 are away from each other. At this time, the sample on the baffles 16 can drip onto the sample pad in the test paper 12.

[0044] The switching mechanism includes a strip groove 26, a slide bar 27, a push block 28 and a positioning assembly for positioning the push block 28. The strip groove 26 is provided at one end of the detection housing 11. The slide bar 27 is inserted into the strip groove 26 and is slidably arranged in the strip groove 26. One end of the slide bar 27 is fixedly connected to the substrate 24, and the other end of the slide bar 27 is fixedly connected to the push block 28. The positioning assembly is located between the push block 28 and the detection housing 11. The positioning assembly can fix the position of the push block 28, and then can realize the fixation of the position of the substrate 24, so as to avoid the substrate 24 from moving randomly and causing the detection test paper 12 to move, thereby ensuring the stability of the detection test paper 12 during the detection process, which is conducive to improving the detection accuracy.

[0045] The replacement component can drive the substrate 24 to move inside the detection shell 11, and then drive the two detection test strips 12 to move, adjust the position of the detection test strips 12, and then facilitate two tests on the sample. This not only avoids the need to take an additional test box for retesting when an operation is incorrect, thereby increasing the test cost, but also the two tests increase the accuracy of the test results.

[0046] like Figure 1-Figure 7 As shown, the moving assembly includes two end plates 17, two mounting plates 18, two guide rods 19 and four second springs 34;

[0047] The two end plates 17 are respectively fixed on the sides of the two baffles 16 that are away from each other, the two mounting plates 18 are symmetrically fixedly installed on both sides of the top of the detection housing 11, and the two guide rods 19 are symmetrically fixed between the two ends of the two mounting plates 18. One end of each end plate 17 is sleeved and slidably set on a guide rod 19, and each two second springs 34 are sleeved on the surface of a guide rod 19, and the two ends of each second spring 34 are respectively fixedly connected to the end plate 17 and the mounting plate 18;

[0048] In the initial state, the two baffles 16 are close to each other and pressed against each other. At this time, the four second springs 34 are all in a compressed but not completely compressed state. When the two baffles 16 need to be moved away from each other, the end plates 17 on both sides are pulled, and the end plates 17 drive the baffles 16 to move, so that the two baffles 16 are moved away from each other. At this time, the second springs 34 are compressed again;

[0049] When the sample on the baffle 16 drops onto the test paper 12 , the end plate 17 is released, and the baffle 16 can be reset under the action of the second spring 34 .

[0050] like Figure 1-Figure 7 As shown, one end of each end plate 17 is fixedly connected to an inclined rod 20, the two inclined rods 20 are staggered, and one end of each inclined rod 20 away from the end plate 17 is fixed to a toggle plate 21;

[0051] During use, when the two end plates 17 need to be moved, the two toggle plates 21 can be pinched by one hand at the same time so that the two toggle plates 21 can be brought closer to each other. At this time, the two end plates 17 can be driven to move under the action of the two inclined rods 20. At this time, the two baffles 16 can be moved simultaneously with one hand.

[0052] like Figure 1-Figure 7 As shown, an inclined scraper 22 for guiding is fixed to the inner wall of the sample adding tube 13, and the bottom end surface of the inclined scraper 22 is in contact with the upper surface of the baffle 16. Specifically, the inclined scraper 22 is made of silicone material. The inclined scraper 22 made of silicone material is arranged inside the sample adding tube 13, which not only plays a role in guiding materials, but also can scrape the surface of the baffle 16 by using the inclined scraper 22 when the two baffles 16 are away from each other, so as to avoid sample residue on the surface of the baffle 16, so that the sample can be more completely dropped on the sample pad on the test paper 12.

[0053] like Figure 1-Figure 7 As shown, a sealing strip is provided on each side of the two baffles 16 close to each other to achieve a sealing effect, which is beneficial to improve the sealing effect between the two baffles 16 and prevent the sample from flowing out from between the two baffles 16 during the metering process.

[0054] like Figure 1-Figure 7 As shown, the positioning assembly includes two countersunk holes 32, two first springs 33, two balls 30 and four positioning grooves 29 matched with the balls 30;

[0055] Two countersunk holes 32 are symmetrically opened on the upper and lower sides of the push block 28. A support frame 31 for mounting the ball 30 is slidably arranged inside each countersunk hole 32. Each ball 30 is mounted inside a support frame 31. The ball 30 can roll inside the support frame 31. That is, when the push block 28 moves, rolling friction is formed between the ball 30 and the detection housing 11, and the friction force is small. Each first spring 33 is fixed inside a countersunk hole 32, and the other end of each first spring 33 is fixedly connected to the support frame 31.

[0056] The four positioning grooves 29 are respectively located on both sides of the push block 28, and the four positioning grooves 29 are symmetrically opened on the detection housing 11, and the two balls 30 are respectively inserted into two of the positioning grooves 29;

[0057] In the initial state, the two balls 30 are respectively located in the two positioning grooves 29 on the same side, and the positioning of the push block 28 can be achieved by the engagement between the balls 30 and the positioning grooves 29;

[0058] When the push block 28 is moved to replace the test paper 12, the push block 28 is pushed. At this time, the ball 30 is disengaged from the inside of the positioning groove 29 and moves into the inside of the countersunk hole 32. The first spring 33 is deformed. When the push block 28 moves to between the two positioning grooves 29 on the other side, under the action of the first spring 33, the ball 30 can be driven to reset, so that the ball 30 can be clamped into the inside of the positioning groove 29 again, thereby positioning the push block 28. At this time, the other test paper 12 is moved to the middle of the detection shell 11, and the adjustment of the position of the test paper 12 is completed.

[0059] like Figure 1-Figure 7 As shown, two guide rails 25 are fixedly installed inside the detection shell 11 and on the side of the substrate 24 away from the test paper 12. The substrate 24 is slidably arranged inside the detection shell 11 through the guide rails 25. The guide rails 25 provide guidance for the substrate 24 so that the substrate 24 can move along a specific direction.

[0060] like Figure 1-Figure 7 As shown, an observation window 14 is provided on the surface of the detection housing 11 for easy observation. The observation window 14 is located above one of the detection test strips 12. Specifically, the observation window 14 is located above the nitrocellulose membrane in one of the detection test strips 12, so as to facilitate observation of the detection data.

[0061] like Figure 1-Figure 7 As shown, a handle 15 that is convenient for holding is fixed to one end of the detection housing 11 away from the pushing block 28 , and the handle 15 is convenient for the user to take the plate test kit.

[0062] like Figure 1-Figure 7 As shown, the surfaces of the handle 15 and the push block 28 are provided with anti-skid stripes for preventing skidding, which is beneficial to improving the friction between the user's hand and the handle 15 or the push block 28, making it easier to use.

[0063] The beneficial effects of the present invention are specifically embodied in that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A calprotectin detection kit, comprising a detection shell (11), It is characterized in that It also comprises a sample adding tube (13), a base plate (24), a metering mechanism, a switching mechanism and two test strips (12), wherein the base plate (24) is slidably arranged inside the detection housing (11), the two test strips (12) are symmetrically arranged above the base plate (24), the sample adding tube (13) is installed at the top end of the detection housing (11), and the sample adding tube (13) is located above one of the test strips (12); The metering mechanism comprises a scale line (23), two baffles (16) and a moving assembly for driving the two baffles (16) to move, the scale line (23) is located on one side of the sample adding tube (13), the two baffles (16) are symmetrically plugged on both sides of the sample adding tube (13), the baffles (16) are slidably connected to the sample adding tube (13), and the moving assembly is located between the sample adding tube (13) and the two baffles (16); The switching mechanism comprises a strip groove (26), a slide rod (27), a push block (28) and a positioning assembly for positioning the push block (28); the strip groove (26) is provided at one end of the detection housing (11); the slide rod (27) is inserted into the strip groove (26) and is slidably arranged in the strip groove (26); one end of the slide rod (27) is fixedly connected to the base plate (24); the other end of the slide rod (27) is fixedly connected to the push block (28); and the positioning assembly is located between the push block (28) and the detection housing (11).

2. A calprotectin detection kit according to claim 1, It is characterized in that The moving assembly comprises two end plates (17), two mounting plates (18), two guide rods (19) and four second springs (34); The two end plates (17) are respectively fixed on the sides of the two baffles (16) away from each other, the two mounting plates (18) are symmetrically fixedly mounted on the two sides of the top of the detection housing (11), the two guide rods (19) are symmetrically fixed between the two ends of the two mounting plates (18), one end of each end plate (17) is sleeved and slidably arranged on a guide rod (19), each of the two second springs (34) is sleeved on the surface of a guide rod (19), and the two ends of each second spring (34) are respectively fixedly connected to the end plate (17) and the mounting plate (18).

3. A calprotectin detection kit according to claim 2, It is characterized in that One end of each end plate (17) is fixedly connected to an inclined rod (20), the two inclined rods (20) are staggered, and one end of each inclined rod (20) away from the end plate (17) is fixed with a toggle plate (21).

4. A calprotectin detection kit according to claim 3, It is characterized in that An inclined scraper (22) for guiding is fixed to the inner wall of the sample adding tube (13), and the bottom end surface of the inclined scraper (22) is in contact with the upper surface of the baffle (16).

5. A calprotectin detection kit according to claim 4, It is characterized in that A sealing strip for achieving a sealing effect is provided on the side where the two baffles (16) are close to each other.

6. A calprotectin detection kit according to claim 5, It is characterized in that The positioning assembly comprises two countersunk holes (32), two first springs (33), two balls (30) and four positioning grooves (29) matched with the balls (30); Two countersunk holes (32) are symmetrically arranged on the upper and lower sides of the push block (28); a support frame (31) for mounting the ball (30) is slidably arranged inside each countersunk hole (32); each ball (30) is mounted inside a support frame (31); each first spring (33) is fixed inside a countersunk hole (32); and the other end of each first spring (33) is fixedly connected to the support frame (31); The four positioning grooves (29) are respectively located on both sides of the push block (28), and the four positioning grooves (29) are symmetrically arranged on the detection housing (11), and the two balls (30) are respectively inserted into the interior of two of the positioning grooves (29).

7. A calprotectin detection kit according to claim 6, It is characterized in that Two guide rails (25) are fixedly installed inside the detection housing (11) and on a side of the base plate (24) away from the detection test paper (12); the base plate (24) is slidably arranged inside the detection housing (11) via the guide rails (25).

8. A calprotectin detection kit according to claim 7, It is characterized in that An observation window (14) is provided on the surface of the detection housing (11) for easy observation, and the observation window (14) is located above one of the detection test papers (12).

9. A calprotectin detection kit according to claim 8, It is characterized in that A handle (15) that is convenient for hand-holding is fixed to one end of the detection housing (11) away from the pushing block (28).

10. A calprotectin detection kit according to claim 9, It is characterized in that The surfaces of the handle (15) and the push block (28) are both provided with anti-skid stripes for preventing skidding.

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