Tangential flow filtration apparatus with sampling device

Abstract

The utility model provides tangential flow filtering equipment with a sampling device, and solves the technical problems that the tangential flow filtering equipment needs to be manually sampled by a worker, the operation is complicated, the sampling accuracy is poor, and pollution is easily caused. The equipment comprises a main circulation container, a filter membrane and a sampling device, an outlet of the main circulation container is communicated with an inlet end of the filter membrane, and a backflow end of the filter membrane is communicated with an inlet of the main circulation container; the sampling device comprises an injection part and a sample receiving container, the sampling pipeline is communicated with the backflow end of the filter membrane and / or the permeation end of the filter membrane, the injection part obtains a sample flowing out of the backflow end of the filter membrane and / or the permeation end of the filter membrane through the sampling pipeline, and the sample can flow into the sample receiving container through the injection part. The sampling pump is started at fixed time or interval time, then liquid in the main circulation container or the permeation end of the filter membrane is sampled, and a sample of the injection part can drop into the sample receiving container, so that the sample is prevented from being polluted, and the change condition of the sample in the technological process is convenient to analyze.

Description

Technical Field

[0001] The utility model relates to the technical field of filtering equipment, in particular to a tangential flow filtering equipment with a sampling device. Background Art

[0002] Tangential flow filtration equipment is an indispensable equipment in the research and development of biological products. It mainly includes a main circulation container, a pump, a filter membrane, a host and a pipeline. The pump pumps the feed liquid in the main circulation container into the filter membrane through the inlet end of the filter membrane, and substances of different molecular weights flow out from the reflux end and the permeation end of the filter membrane respectively. The feed liquid is processed by the tangential flow filtration equipment. During the processing, it is necessary to continuously understand the changes in the feed liquid. Therefore, it is necessary to sample the feed liquid at regular intervals.

[0003] The traditional method requires manual sampling in the main circulation container of the tangential flow filtration or the permeation end of the filter membrane, and then analyzing the extracted liquid to adjust the tangential flow filtration equipment based on the analysis results.

[0004] The applicant has found that the prior art has at least the following technical problems: the above method, during multiple manual sampling processes, requires experimenters to take samples according to time, which sometimes leads to errors. In addition, the accuracy of the sampling volume is difficult to control, affecting the accuracy of the experimental data. Human operation increases the risk of contamination and pollution. Utility Model Content

[0005] The purpose of the utility model is to provide a tangential flow filtration device with a sampling device to solve the technical problems in the prior art that the tangential flow filtration device requires manual sampling by staff, the operation is cumbersome, the sampling accuracy is poor and it is easy to cause pollution; the many technical effects that can be produced by the preferred technical scheme among the many technical schemes provided by the utility model are detailed as follows.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] The utility model provides a tangential flow filtration device with a sampling device, comprising a main circulation container, a filter membrane and a sampling device, wherein:

[0008] The outlet of the main circulation container is communicated with the inlet end of the filter membrane, and the reflux end of the filter membrane is communicated with the inlet of the main circulation container; the sampling device includes an injection part and a sample receiving container, the sampling device is communicated with the reflux end of the filter membrane and / or the permeation end of the filter membrane, the injection part obtains the sample flowing out of the reflux end of the filter membrane and / or the permeation end of the filter membrane through the sampling device, and the sample can flow into the sample receiving container through the injection part.

[0009] Preferably, the tangential flow filtration device further comprises a permeate end container, the permeate end container is connected to the permeate end of the filter membrane, and the sampling device is connected to the main circulation container and / or the permeate end container.

[0010] Preferably, the sampling device is provided with a sampling pump.

[0011] Preferably, the driving assembly comprises a frame, a first linear servo module, a guide rail frame and a second linear servo module, wherein:

[0012] The frame is located beside the filter membrane, the first linear servo module is arranged along the X-axis direction, the guide rail frame is fixed on the slider of the first linear servo module, the second linear servo module is located on the guide rail frame and arranged along the Y-axis direction, and the injection part is fixed on the slider of the second linear servo module.

[0013] Preferably, the tangential flow filtration device further comprises a main controller, which is electrically connected to the sampling pump and is used to control the sampling pump to be turned on or off.

[0014] Preferably, the tangential flow filtration device further comprises a main body, the main controller is located on the main body, and the filter membrane is fixed on the main body.

[0015] Preferably, the tangential flow filtration device further comprises an infusion pump, which is connected to a pipeline between the main circulation container and the filter membrane and is used to pump the feed liquid in the main circulation container into the filter membrane through the inlet end of the filter membrane.

[0016] Preferably, the filter membrane comprises a hollow fiber membrane or a plate membrane package.

[0017] Preferably, the main circulation container comprises a tank body.

[0018] The tangential flow filtration equipment with a sampling device provided by the utility model has the following beneficial effects compared with the prior art: the outlet of the main circulation container and / or the permeable end of the filter membrane is connected to the injection part through the sampling device, and a sampling pump is arranged on the sampling device. The sampling pump can be turned on at a fixed time or interval time according to the experimental requirements to sample the liquid in the main circulation container or the permeable end of the filter membrane, and the sample of the injection part can be dripped into the sample receiving container, and there is no need to manually sample from the outlet of the main circulation container and / or the permeable end of the filter membrane, thereby preventing contamination of the sample and facilitating the analysis of sample changes in the process. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 It is a schematic diagram of the structure of a tangential flow filtration device with a sampling device;

[0021] Figure 2 It is a schematic diagram of the connection between the sampling device and the permeable end of the filter membrane.

[0022] In the figure, 1 is the main circulation container; 2 is the filter membrane; 21 is the permeate end; 22 is the inlet end; 23 is the reflux end; 100 is the sampling device; 101 is the sampling device; 3 is the injection part; 4 is the test tube rack; 51 is the frame; 52 is the guide rail rack; 6 is the infusion pump; 7 is the main body; 8 is the permeate end container. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution of the utility model will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the utility model.

[0024] In the description of the present invention, it should be understood that the terms "center", "length", "width", "height", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "side" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In the description of the present invention, unless otherwise specified, "multiple" means two or more.

[0025] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0026] The embodiment of the utility model provides a tangential flow filtration device with a sampling device, which can start the sampling pump at a fixed time or a certain interval according to the experimental requirements, and then sample the liquid in the main circulation container or the permeation end of the filter membrane.

[0027] Combine the following Figure 1 The technical solution provided by the utility model is described in more detail.

[0028] like Figure 1 As shown, the tangential flow filtration equipment with a sampling device provided by the utility model comprises a main circulation container 1, a filter membrane 2 and a sampling device 100, wherein: the outlet of the main circulation container 1 is communicated with the inlet end 22 of the filter membrane 2, and the reflux end 23 of the filter membrane 2 is communicated with the inlet of the main circulation container 1; the sampling device 100 comprises an injection part 3 and a sample receiving container, the injection part 3 is connected to the outlet of the main circulation container 1 and / or the permeable end 21 of the filter membrane 2 through the sampling device 101, and a sampling pump is arranged on the sampling device 100, and the sample of the injection part 3 can drip into the sample receiving container.

[0029] See also Figure 2 As shown, Figure 2 In the embodiment, the injection part 3 is connected to the permeation end 21 of the filter membrane 2 through the sampling device 101. The permeation end of the filter membrane 2 is also connected to the permeation end container 8 through the permeation end pipeline, and the sampling device 101 is connected to the permeation end pipeline through a three-way valve.

[0030] When the sampling pump is turned on, the sampling device 101 is connected to the main circulation container and the injection part 3 , or is connected to the permeation end 21 of the filter membrane 2 and the injection part 3 .

[0031] The sampling device is provided at least at one of the outlet end of the main circulation container 1 and the permeable end 21 of the filter membrane 2. Specifically, the sampling device is provided only at the outlet end of the main circulation container 1, or the sampling device is provided only at the permeable end 21 of the filter membrane 2, or one sampling device is provided at both the outlet end of the main circulation container 1 and the permeable end 21 of the filter membrane 2, or corresponding sampling devices are provided at both the outlet end of the main circulation container 1 and the permeable end 21 of the filter membrane 2, a sampling pump is provided on each sampling device, and two sampling devices are connected to an injection part 3.

[0032] See also Figure 1 As shown, the sampling device is omitted in this embodiment. Taking the example of connecting the sampling device to the permeable end 21 of the filter membrane 2, the two ends of the sampling device are connected to the outlet of the main circulation container and the inlet of the injection part, and the sampling pump is connected to the sampling device.

[0033] The sampling pump is a liquid delivery pump in the prior art, and its structure is not described in detail here.

[0034] See also Figure 1 As shown, the injection part 3 includes an injection head, and the sampling pump pumps the sample liquid at the outlet end of the main circulation container 1 or the sample liquid at the permeation end 21 of the filter membrane 2 into the injection part 3, and the sample liquid in the injection part 3 drips into the sample receiving container, thereby facilitating the analysis of the sample.

[0035] Because the reflux end of the filter membrane 2 is directly connected to the main circulation container 1 through a pipeline, and the permeate end of the filter membrane 2 is directly connected to the permeate end container through a pipeline, therefore, according to the actual needs of pipeline layout and equipment placement, the sampling device can be connected to the main circulation container and / or the permeate end container, and the sampling device can also be connected to the reflux end pipeline and / or the permeate end pipeline.

[0036] In the tangential flow filtration equipment with a sampling device of this embodiment, the outlet of the main circulation container 1 and / or the permeable end 21 of the filter membrane 2 are connected to the injection part 3 through the sampling device. A sampling pump is arranged on the sampling device. The sampling pump can be turned on at a fixed time or interval according to the experimental requirements to sample the liquid in the main circulation container 1 or the permeable end 21 of the filter membrane 2. The sample of the injection part 3 can drip into the sample receiving container, and there is no need to manually sample from the outlet of the main circulation container 1 and / or the permeable end 21 of the filter membrane 2, so as to prevent contamination of the sample and facilitate the analysis of the change of the sample in the process.

[0037] As an optional embodiment, the tangential flow filtration device further includes a main controller, which is electrically connected to the sampling pump and is used to control the sampling pump to be turned on or off.

[0038] The pump body of the sampling device is electrically connected to the main controller of the tangential flow filtration equipment. The sampling pump is controlled to be turned on or off by the main controller. When the sampling pump is turned on, the sampling device realizes automatic sampling, which not only saves labor, but also can automatically perform timed and quantitative sampling with high accuracy to avoid contamination of the feed liquid.

[0039] As an alternative embodiment, see Figure 1 The tangential flow filtration device further comprises a main body 7, a main controller is located on the main body 7, and a filter membrane 2 is fixed on the main body 7. The filter membrane 2 can be fixed on the main body 7 by a snap connection or the like, so as to facilitate the fixing of the filter membrane 2.

[0040] As an alternative embodiment, see Figure 1 As shown, the sample receiving container includes a test tube rack 4 and a test tube, and the test tube is placed in a card slot of the test tube rack 4.

[0041] The sample liquid in the injection part 3 can be directly dripped into the test tube of the test tube rack 4, which is convenient for collecting the sample liquid and facilitating multiple and regular quantitative sampling of small amounts.

[0042] As an optional implementation, the tangential flow filtration device of this embodiment also includes a driving component, which is transmission-connected to the injection part 3 for driving the injection part 3 to move, or the driving component is transmission-connected to the sample receiving container for driving the sample receiving container to move.

[0043] Here are two specific ways to implement it:

[0044] Embodiment 1

[0045] The test tube stand 4 is fixed, and the injection part 3 can move in the horizontal direction to inject the liquid into different test tubes. Furthermore, the injection part 3 can also move in the vertical direction to prevent leakage during the process of injecting the liquid into the test tube.

[0046] See also Figure 1 The driving assembly includes a frame 51, a first linear servo module, a guide frame 52 and a second linear servo module, wherein: the frame 51 is located beside the filter membrane 2, the first linear servo module is arranged along the X-axis direction, the guide frame 52 is fixed on the slider of the first linear servo module, the second linear servo module is located on the guide frame 52 and is arranged along the Y-axis direction, and the injection part 3 is fixed on the slider of the second linear servo module.

[0047] The first linear servo module and the second linear servo module are existing mature technologies, and their structures are not described here, and they can be directly purchased from the market. By setting the first linear servo module and the second linear servo module in different directions, the above structure can realize the movement of the injection part 3 along the X-axis direction (horizontal direction) and the Y-axis direction (vertical direction).

[0048] Embodiment 2

[0049] The injection part 3 is fixedly arranged above the test tube rack 4, and the test tube rack 4 can move in the horizontal direction to align different test tubes with the injection part 3, so as to facilitate the injection of liquid into the test tube. Further, the test tube rack 4 can also move in the vertical direction to prevent leakage during the injection of liquid.

[0050] The driving assembly can also adopt the structure of the first embodiment, and the test tube rack 4 can be fixed on the slider of the second linear servo module.

[0051] As an alternative embodiment, see Figure 1As shown, the tangential flow filtration device also includes an infusion pump 6, which is connected to the pipeline between the main circulation container 1 and the filter membrane 2, and is used to pump the feed liquid in the main circulation container 1 into the filter membrane 2 through the inlet end 22 of the filter membrane 2.

[0052] The infusion pump 6 can be fixed on the main body 7. The infusion pump 6 can pump the feed liquid in the main circulation container 1 into the filter membrane 2 through the inlet end 22 of the filter membrane 2, so that the feed liquid can smoothly enter the filter membrane 2 for filtration.

[0053] As an optional embodiment, the filter membrane 2 includes a hollow fiber membrane or a plate-type membrane package.

[0054] As an optional embodiment, the primary circulation container 1 includes a tank.

[0055] The tangential flow filtration equipment with a sampling device can be set in the main controller to automatically sample at a certain time interval according to the needs, or it can be set to sample according to the weight change of the liquid in the main circulation container 1. Subsequently, the sampling device samples the liquid to be sampled into the test tube on time or according to the weight change under the control of the main controller, realizing unattended sampling.

[0056] In the description of this specification, specific features, structures or characteristics may be combined in an appropriate manner in any one or more embodiments or examples.

[0057] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0058] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed by the utility model, which should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.

Claims

1. A tangential flow filtration device with a sampling device, characterized in that: It includes a main circulation container, a filter membrane and a sampling device, wherein: The outlet of the main circulation container is communicated with the inlet end of the filter membrane, and the reflux end of the filter membrane is communicated with the inlet of the main circulation container; the sampling device includes an injection part and a sample receiving container, the sampling device is communicated with the reflux end of the filter membrane and / or the permeation end of the filter membrane, the injection part obtains the sample flowing out of the reflux end of the filter membrane and / or the permeation end of the filter membrane through the sampling device, and the sample can flow into the sample receiving container through the injection part.

2. The tangential flow filtration device with a sampling device according to claim 1, characterized in that: The tangential flow filtration device further comprises a permeate end container, the permeate end container is connected to the permeate end of the filter membrane, and the sampling device is connected to the main circulation container and / or the permeate end container.

3. The tangential flow filtration device with a sampling device according to claim 1, characterized in that: The sampling device is provided with a sampling pump.

4. The tangential flow filtration device with a sampling device according to claim 1, characterized in that: The sample receiving container comprises a test tube rack and a test tube, and the test tube is placed in a card slot of the test tube rack.

5. The tangential flow filtration device with a sampling device according to claim 1, characterized in that: The tangential flow filtration device also includes a driving component, which is in driving connection with the injection part for driving the injection part to move, or the driving component is in driving connection with the sample receiving container for driving the sample receiving container to move.

6. The tangential flow filtration device with a sampling device according to claim 5, characterized in that: The driving assembly includes a frame, a first linear servo module, a guide rail frame and a second linear servo module, wherein: The frame is located beside the filter membrane, the first linear servo module is arranged along the X-axis direction, the guide rail frame is fixed on the slider of the first linear servo module, the second linear servo module is located on the guide rail frame and arranged along the Y-axis direction, and the injection part is fixed on the slider of the second linear servo module.

7. The tangential flow filtration device with a sampling device according to claim 3, characterized in that: The tangential flow filtration device further comprises a main controller, which is electrically connected to the sampling pump and is used to control the sampling pump to be turned on or off.

8. The tangential flow filtration device with a sampling device according to claim 7, characterized in that: The tangential flow filtration device further comprises a main body, the main controller is located on the main body, and the filter membrane is fixed on the main body.

9. The tangential flow filtration device with a sampling device according to claim 1, characterized in that: The tangential flow filtration device also includes an infusion pump, which is connected to the pipeline between the main circulation container and the filter membrane and is used to pump the feed liquid in the main circulation container into the filter membrane through the inlet end of the filter membrane.

10. The tangential flow filtration device with a sampling device according to claim 1, characterized in that: The filter membrane includes a hollow fiber membrane or a plate-type membrane package.

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