Micro-fluidic PCR chip heating device
The microfluidic PCR chip heating device addresses the limitations of single-chip heating by allowing multiple chips to be heated simultaneously and accelerating temperature transitions, thus reducing evaporation and improving reaction efficiency.
Patent Information
- Application Number
- CN202420932926.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-30
- Publication Date
- 2024-12-24
- Estimated Expiration
- 2034-04-30
AI Technical Summary
Existing microfluidic PCR chip heating devices can only heat one chip at a time, leading to prolonged experimental times and evaporation of culture medium, and often experience slow temperature transitions between stages, affecting reaction speed and efficiency.
A microfluidic PCR chip heating device designed to accommodate multiple chips simultaneously, featuring a chip box with a turning handle and threaded rod system, which includes a threaded handle and threaded rod system, and incorporates a fan and an opening, with a fan and an opening, with a fan and an opening, that accelerates the transition between the denaturation stage and the annealing stage, and the annealing stage, and the annealing stage, and the annealing stage, reducing evaporation and improving cooling speed.
Enables simultaneous heating of multiple chips, reduces evaporation, and accelerates temperature transitions, thereby shortening reaction time and enhancing efficiency.
Smart Images
Figure CN222226383U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of heating devices, in particular to a microfluidic PCR chip heating device. Background Art
[0002] The microfluidic PCR chip heating device is a device used to perform PCR reactions at a microscale. The design of the microfluidic PCR chip heating device is to achieve highly accurate temperature control and PCR reactions under small sample volumes. They are commonly used in biomedicine, gene detection, pathogen diagnosis, gene expression analysis and other fields. They are fast, efficient and accurate, and can be highly automated. The basic principle of PCR (polymerase chain reaction) technology is similar to the natural replication process of DNA. Its specificity depends on the oligonucleotide primers that are complementary to both ends of the target sequence. PCR is basically composed of three basic reaction steps: denaturation-annealing-extension. PCR uses the fact that DNA denatures at a high temperature of 95°C in vitro to become a single strand. When the low temperature is often around 60°C, the primer and the single strand combine according to the principle of base complementary pairing. The temperature is then adjusted to the optimal reaction temperature of DNA polymerase, which is around 72°C. DNA polymerase synthesizes complementary chains along the direction from phosphate to pentose.
[0003] In the prior art, most instruments can only heat one chip at a time, which increases the time for experiments to conduct reactions on multiple groups of chips. Some instruments will evaporate the culture medium in the chip to a certain extent during heating, making the culture medium inside the chip insufficient to support the number of reactions. In the process of switching from the denaturation stage to the annealing stage, the temperature of some instruments drops too slowly, affecting the reaction speed. Utility Model Content
[0004] The utility model aims to provide a microfluidic PCR chip heating device, which can heat multiple chips at the same time and reduce the evaporation of culture solution, and can shorten the time of switching from the denaturation stage to the annealing stage.
[0005] To achieve the above-mentioned purpose, a microfluidic PCR chip heating device is provided, comprising a box body, a chip box is arranged in front of the box body, a nut seat is fixedly connected to the upper surface of the box body, a threaded rod is connected to the internal thread of the nut seat, the upper end of the threaded rod is fixedly connected to a turning handle, the lower end of the threaded rod is rotatably connected to an axle seat, the lower surface of the axle seat is fixedly connected to an upper pressing plate, the lower surface of the upper pressing plate is fixedly connected to an upper heating block, the lower surface of the upper heating block is fixedly connected to the chip box, the lower surface of the chip box is fixedly connected to a lower heating block, the lower surface of the lower heating block is fixedly connected to a lower pressing plate, the lower surface of the lower pressing plate is fixedly connected to a buffer spring, the lower end of the buffer spring is fixedly connected to the box body, a fan is arranged on the left side of the box body, and an opening is arranged on the right side of the box body.
[0006] According to the microfluidic PCR chip heating device, the front middle portion of the box body is rotatably connected to a door body, the front left side of the box body is fixedly connected to a lock block, and the front right side of the door body is rotatably connected to a stopper.
[0007] According to the microfluidic PCR chip heating device, grooves are provided on both sides of the inner side of the box body, both sides of the upper pressing plate are slidably connected to the inner side of the box body, and both sides of the lower pressing plate are slidably connected to the inner side of the box body.
[0008] According to the microfluidic PCR chip heating device, an upper closing chamber is arranged above the chip box, a movable chamber is slidably connected to the lower side of the upper closing chamber, a support spring is fixedly connected to the lower side of the movable chamber, a lower end of the support spring is fixedly connected to the lower closing chamber, and a sliding groove is provided on the frame of the lower closing chamber.
[0009] According to the microfluidic PCR chip heating device, the support spring is located inside the slide groove.
[0010] According to the microfluidic PCR chip heating device, the lower portion of the movable chamber is located inside the slide groove, and the movable chamber is slidably connected to the slide groove.
[0011] According to the microfluidic PCR chip heating device, the upper closing chamber is slidably connected to the lower closing chamber.
[0012] The utility model has the following beneficial effects:
[0013] 1. Compared with the prior art, the microfluidic PCR chip heating device is provided with a chip box, in which chips can be placed. Since the internal area of the chip box is large, multiple chips can be placed at the same time, so that multiple chips can be heated at the same time. By providing a turning handle, the threaded rod can be manually controlled to rotate, so that the threaded rod pushes the shaft seat to move up and down, and the shaft seat links the upper pressure plate to move up and down at the same time, thereby pushing the chip box up and down, so that the inner upper surface of the chip box is against the upper surface of the chip, thereby reducing the evaporation of the culture solution in the chip.
[0014] 2. Compared with the prior art, this microfluidic PCR chip heating device can accelerate the cooling process by providing a fan and an opening, that is, accelerate the transition between the denaturation stage and the annealing stage, reduce the reaction time and improve the reaction efficiency.
[0015] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] The utility model is further described below in conjunction with the accompanying drawings and embodiments;
[0017] Figure 1 This is a three-dimensional diagram of a microfluidic PCR chip heating device of the utility model;
[0018] Figure 2 This is a front view of a microfluidic PCR chip heating device of the utility model;
[0019] Figure 3 This is a cross-sectional view of a microfluidic PCR chip heating device of the utility model;
[0020] Figure 4 This is a chip box disassembly diagram of a microfluidic PCR chip heating device of the utility model;
[0021] Figure 5 The utility model is a schematic diagram of a locking block of a microfluidic PCR chip heating device.
[0022] Legend:
[0023] 1. Box body; 2. Door body; 3. Stop handle; 4. Lock block; 5. Nut seat; 6. Threaded rod; 7. Turn handle; 8. Shaft seat; 9. Upper pressure plate; 10. Upper heating block; 11. Chip box; 12. Lower heating block; 13. Lower pressure plate; 14. Buffer spring; 15. Groove; 16. Fan; 17. Opening;
[0024] 111. upper closing chamber; 112. movable chamber; 113. supporting spring; 114. slide slot; 115. lower closing chamber. DETAILED DESCRIPTION
[0025] This section will describe in detail the specific embodiments of the utility model. The preferred embodiments of the utility model are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the utility model, but it cannot be understood as a limitation on the protection scope of the utility model.
[0026] Reference Figure 1-5The utility model embodiment is a microfluidic PCR chip heating device, which includes a box body 1, a chip box 11 is arranged in front of the box body 1, a door body 2 is rotatably connected to the middle part of the front of the box body 1, a lock block 4 is fixedly connected to the left side of the front of the box body 1, and a stopper 3 is rotatably connected to the right side of the front of the door body 2, a nut seat 5 is fixedly connected to the upper surface of the box body 1, a threaded rod 6 is internally threadedly connected to the nut seat 5, a handle 7 is fixedly connected to the upper end of the threaded rod 6, and an axle seat 8 is rotatably connected to the lower end of the threaded rod 6, an upper pressing plate 9 is fixedly connected to the lower surface of the axle seat 8, an upper heating block 10 is fixedly connected to the lower surface of the upper heating block 10, and a chip box 11 is fixedly connected to the lower surface of the chip box 11, and an upper closing chamber 111 is arranged above the chip box 11, and the upper closing chamber 111 slides with the lower closing chamber 115 The movable bin 112 is slidably connected to the lower side of the upper closing bin 111, and a support spring 113 is fixedly connected to the lower side of the movable bin 112. The lower end of the support spring 113 is fixedly connected to the lower closing bin 115. The frame of the lower closing bin 115 is provided with a slide groove 114. The support spring 113 is located inside the slide groove 114. The lower part of the movable bin 112 is located inside the slide groove 114. The movable bin 112 is slidably connected to the slide groove 114. The lower surface of the chip box 11 is fixedly connected to the lower heating block 12, and the lower surface of the lower heating block 12 is fixedly connected to the lower pressure plate 13. The lower surface of the lower pressure plate 13 is fixedly connected to the buffer spring 14, and the lower end of the buffer spring 14 is fixedly connected to the box body 1. A fan 16 is provided on the left side of the box body 1, and an opening 17 is provided on the right side of the box body 1.
[0027] The above structure, by providing a chip box 11, can place chips inside the chip box. Since the internal area of the chip box 11 is large, multiple chips can be placed at the same time, so that the multiple chips can be heated at the same time. By providing a door body 2, a closed space can be formed inside the chip box 11 when the door body 2 is closed. By providing a stopper 3 and a lock block 4, when the stopper 3 is turned to the position of the lock block 4, the stopper 3 can lock the door body 2 to prevent it from opening during operation due to external force.
[0028] By providing the upper heating block 10 and the lower heating block 12 , the chip box 11 can be heated, and then the chips inside the chip box 11 can be heated.
[0029] By providing a turning handle 7, the threaded rod 6 can be manually controlled to rotate, so that the threaded rod 6 pushes the shaft seat 8 to move up and down, and the shaft seat 8 links the upper pressure plate 9 to move up and down at the same time, thereby pushing the chip box 11 to move up and down, so that the inner upper surface of the chip box 11 is against the upper surface of the chip, reducing the evaporation of the culture solution in the chip, and the buffer spring 14 can play a buffering role when the inner upper surface of the chip box 11 contacts the upper surface of the chip, preventing damage to the chip due to operational errors.
[0030] By providing the fan 16 and the opening 17, the cooling process can be accelerated, that is, the transition between the denaturation stage and the annealing stage can be accelerated, the reaction time can be reduced, and the reaction efficiency can be improved.
[0031] The support spring 113 can provide support for the upper closing chamber 111, so that the chip box 11 can reach the maximum opening and closing area when the upper closing chamber 111 is not subjected to external force.
[0032] Grooves 15 are provided on both sides of the inner side of the box body 1 , both sides of the upper pressing plate 9 are slidably connected to the inner side of the box body 1 , and both sides of the lower pressing plate 13 are slidably connected to the inner side of the box body 1 .
[0033] The above structure can limit the upper pressure plate 9 by opening the groove 15 so that it will not deviate left or right when moving up and down. The lower pressure plate 13 can be limited by opening the groove 15 so that it will not deviate left or right when performing buffering operation.
[0034] Working principle: When the microfluidic PCR chip heating device is working, first put the chip into the chip box 11, turn the handle 7 to lower the upper pressure plate 9, push the upper closing chamber 111 to make its inner upper surface fit the upper surface of the chip, close the door 2, turn the stopper 3 to place the other side of it in the lock block 4, and turn on the switches of the upper heating block 10 and the lower heating block 12 to heat the chip box 11. The chip box 11 conducts heat to the chip to achieve heating of the chip. When the denaturation stage inside the chip is completed, the temperature of the two heating blocks is lowered, and the fan 1 is turned on at this time. The switch of 6 accelerates the cooling speed of the chip box 11, that is, accelerates the cooling speed of the chip, until the chip drops to the temperature required for the annealing stage, then turns off the switch of the fan 16, and the chip enters the annealing stage. After the annealing stage is over, adjust the temperature of the two heating blocks to make the chip enter the extension stage. After the extension stage is over, increase the temperature of the two heating blocks to make the chip continue to the deformation stage. At this point, a cycle is ended. After several cycles, when the experimental purpose is achieved, turn off the switches of the two heating blocks and let them stand for a certain period of time, then open the door 2, take out the chip, and the work is completed.
[0035] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge scope of ordinary technicians in the technical field without departing from the purpose of the present invention.
Claims
1. A microfluidic PCR chip heating device, characterized in that: The invention comprises a box body (1), a chip box (11) is arranged in front of the box body (1), a nut seat (5) is fixedly connected to the upper surface of the box body (1), a threaded rod (6) is internally threadedly connected to the nut seat (5), a handle (7) is fixedly connected to the upper end of the threaded rod (6), a shaft seat (8) is rotatably connected to the lower end of the threaded rod (6), an upper pressing plate (9) is fixedly connected to the lower surface of the shaft seat (8), an upper heating block (10) is fixedly connected to the lower surface of the upper pressing plate (9), and the The lower surface of the upper heating block (10) is fixedly connected to a chip box (11), the lower surface of the chip box (11) is fixedly connected to a lower heating block (12), the lower surface of the lower heating block (12) is fixedly connected to a lower pressure plate (13), the lower surface of the lower pressure plate (13) is fixedly connected to a buffer spring (14), the lower end of the buffer spring (14) is fixedly connected to the box body (1), a fan (16) is arranged on the left side of the box body (1), and an opening (17) is arranged on the right side of the box body (1).
2. A microfluidic PCR chip heating device according to claim 1, characterized in that: The front middle part of the box body (1) is rotatably connected to the door body (2), the front left side of the box body (1) is fixedly connected to a lock block (4), and the front right side of the door body (2) is rotatably connected to a stop handle (3).
3. A microfluidic PCR chip heating device according to claim 1, characterized in that: Grooves (15) are provided on both sides of the inner side of the box body (1), both sides of the upper pressing plate (9) are slidably connected to the inner side of the box body (1), and both sides of the lower pressing plate (13) are slidably connected to the inner side of the box body (1).
4. A microfluidic PCR chip heating device according to claim 1, characterized in that: An upper closing bin (111) is arranged above the chip box (11), a movable bin (112) is slidably connected to the lower side of the upper closing bin (111), a support spring (113) is fixedly connected to the lower side of the movable bin (112), a lower end of the support spring (113) is fixedly connected to the lower closing bin (115), and a sliding groove (114) is provided on the frame of the lower closing bin (115).
5. A microfluidic PCR chip heating device according to claim 4, characterized in that: The support spring (113) is located inside the slide groove (114).
6. A microfluidic PCR chip heating device according to claim 4, characterized in that: The lower portion of the movable bin (112) is located inside the slide groove (114), and the movable bin (112) is slidably connected to the slide groove (114).
7. A microfluidic PCR chip heating device according to claim 4, characterized in that: The upper closing chamber (111) is slidably connected to the lower closing chamber (115).