Miniaturized heparin extraction equipment

Abstract

The utility model provides miniaturized heparin extraction equipment. The miniaturized heparin extraction equipment comprises a pulping box; the cutting pump is arranged in the pulping box, one side of the cutting pump is fixedly connected with a hydraulic pipe, the bottom of one side of the outer surface of the pulping box is provided with a hand valve, one side in the pulping box is provided with a return pipe, the other end of the return pipe is fixedly connected with a self-priming pump, and the other end of the hydraulic pipe is fixedly connected with an enzymolysis tank. The utility model provides miniaturized heparin extraction equipment, which realizes a complete process mode of producing while putting into production, equipment miniaturization, a production mode of producing while electrifying and introducing water at any time and any place and a single machine full flow, realizes production while putting into production, adopts a quick connection mode for all structures of the equipment, and is convenient to mount, dismount and maintain.

Description

Technical Field

[0001] The utility model relates to the field of heparin extraction, in particular to a miniaturized heparin extraction device. Background Art

[0002] Heparin sodium is an acidic mucopolysaccharide sulfate substance produced by mast cells in animal connective tissue. It is widely present in the liver, lungs, and intestinal mucosa of mammals. It is mostly combined with proteins to form a complex. Because of its strong anticoagulant effect, it is the first choice drug for preventing and treating thromboembolic diseases such as deep vein thrombosis.

[0003] As the research deepened, people found that sodium heparin not only has the effects of anticoagulation, antithrombosis and regulating blood lipids, but also has multiple biological functions such as anti-inflammatory, anti-allergic and anti-cancer. It is the most important anticoagulant and anti-thrombotic biochemical drug. At present, it can only be extracted from some animal tissues and cannot be synthesized artificially. The existing extraction method extracts sodium heparin from the pig small intestinal mucosa, and the extraction equipment is a large-scale factory modular production method. There is currently no corresponding small equipment complete solution for the distributed discrete market.

[0004] Therefore, it is necessary to provide a miniaturized heparin extraction device to solve the above technical problems. Utility Model Content

[0005] The utility model provides a miniaturized heparin extraction device, which solves the problem that the existing extraction method for extracting sodium heparin from the pig small intestinal mucosa is that the extraction equipment is produced in a modular manner in a large factory, and there is no corresponding complete set of small equipment solutions for the distributed discrete market.

[0006] In order to solve the above technical problems, the utility model provides a miniaturized heparin extraction device, comprising: a beating box;

[0007] A cutting pump, wherein the cutting pump is arranged inside the beating box, a hydraulic pipe is fixedly connected to one side of the cutting pump, a hand valve is arranged at the bottom of one side of the outer surface of the beating box, a return pipe is arranged on one side inside the beating box, a self-priming pump is fixedly connected to the other end of the return pipe, and an enzymolysis tank is fixedly connected to the other end of the hydraulic pipe;

[0008] A three-way pipe, wherein the three-way pipe is arranged on one side of the top of the enzymolysis tank, one end of the three-way pipe is fixedly connected to an adsorption column, one side of the outer surface of the adsorption column is fixedly installed with a sewage valve, one side of the adsorption column is connected to a high-efficiency filter column through a pipeline, and one side of the high-efficiency filter column is connected to a low-efficiency filter column through a pipeline;

[0009] A heating tube is arranged at the bottom of the beating box.

[0010] Preferably, a stirring motor is fixedly installed in the middle of the top of the enzymolysis tank, and a stirrer is fixedly connected to the bottom end of the output shaft of the stirring motor.

[0011] Preferably, three heating tubes are provided.

[0012] Preferably, a discharge valve is provided at the bottom of the beating box, and the hand valve is connected to the beating box and the enzymolysis tank through a pipeline.

[0013] Preferably, a slide groove is opened in the middle of one side of the outer surface of the beating box, a slide rod is fixedly installed inside the slide groove, an elastic part is sleeved on the bottom of the outer surface of the slide rod, a moving device is sleeved on the top of the outer surface of the slide rod, a cleaning device is fixedly installed on one side of the bottom of the moving device, and a mounting frame is fixedly installed in the middle of the top of the beating box.

[0014] Preferably, the cleaning device comprises a movable box, a cleaning block is fixedly mounted on the bottom of the movable box, and a connecting groove is opened in the middle of the top of the movable box.

[0015] Preferably, the moving device comprises a connecting rod, a fixing rod is fixedly mounted on the top of one end of the connecting rod, a mounting rod is fixedly mounted on the bottom of one side of the fixing rod, and a sliding block is fixedly mounted on the bottom of one side of the connecting rod.

[0016] Preferably, the output shaft of the stirring motor is fixedly connected to a first stirring device, and second stirring devices are meshed on both sides of the first stirring device.

[0017] Preferably, the first stirring device comprises an active rod, a first stirring plate is fixedly mounted on the outer surface of the active rod, and a driving gear is fixedly mounted on the top of the outer surface of the active rod.

[0018] Preferably, the second stirring device comprises a rotating shaft, a second stirring plate is fixedly mounted on the outer surface of the rotating shaft, and a driven gear is fixedly mounted on the top of the outer surface of the rotating shaft.

[0019] Compared with the related art, the miniaturized heparin extraction device provided by the utility model has the following advantages:

[0020] Beneficial effects:

[0021] The utility model provides a miniaturized heparin extraction device. After the pig small intestine is poured into a pulping box through a stainless steel rotary cutting pump, a cutting and extraction method is adopted to cooperate with an enzymolysis tank for a cycle to form a cyclic pulping process, thereby solving the problems of high cost and large floor space of a traditional crusher. A low-efficiency filtration + high-efficiency filtration + resin adsorption through-linked production mode is adopted, and the small intestine solution after enzymolysis is directly adsorbed by the resin in one process. At the same time, the method is cyclically adsorbed and settled to the bottom to extract all the heparin in the small intestine of the small batch produced pig, thereby realizing a complete process mode of output at the same time when it is put into production. The equipment is miniaturized, and production can be carried out anytime and anywhere as long as it is powered on and water is supplied. The single-machine full-process production mode realizes input and output, and all structural installations of the equipment are quick-connected, which is convenient for installation, disassembly and maintenance. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 A schematic structural diagram of a first embodiment of a miniaturized heparin extraction device provided by the utility model;

[0023] Figure 2 for Figure 1 Bottom view of the enzymatic hydrolysis tank shown;

[0024] Figure 3 A schematic structural diagram of a second embodiment of a miniaturized heparin extraction device provided by the utility model;

[0025] Figure 4 for Figure 3 An enlarged schematic diagram of point A is shown;

[0026] Figure 5 for Figure 3 The schematic diagram of the mobile device structure is shown.

[0027] Figure 6 A schematic structural diagram of a third embodiment of a miniaturized heparin extraction device provided by the utility model;

[0028] Figure 7 for Figure 6 An enlarged schematic diagram of point B is shown.

[0029] Numbers in the figure: 1. beating box, 2. cutting pump, 3. hydraulic pipe, 4. discharge valve, 5. self-priming pump, 6. enzymolysis tank, 7. low-efficiency filter column, 8. high-efficiency filter column, 9. adsorption column, 10. drain valve, 11. three-way valve, 12. stirring motor, 13. reflux pipe, 14. hand valve, 15. heating pipe, 16. slide groove, 17. slide rod, 18. elastic member, 19. moving device, 191. connecting rod, 192. fixing rod, 193. mounting rod, 194. slider, 20. cleaning device, 201. moving box, 202. connecting groove, 203. cleaning block, 21. mounting frame, 22. stirring chamber, 23. first stirring device, 231. active rod, 232. active gear, 233. first stirring plate, 24. second stirring device, 241. rotating shaft, 242. driven gear, 243. second stirring plate. DETAILED DESCRIPTION

[0030] The utility model is further described below in conjunction with the accompanying drawings and implementation modes.

[0031] Please refer to Figure 1 , Figure 2 and Figure 3 ,in, Figure 1 A schematic structural diagram of a first embodiment of a miniaturized heparin extraction device provided by the utility model; Figure 2 for Figure 1 The schematic diagram of the coffee bag structure shown; Figure 3 for Figure 1 The filter cup structure schematic diagram is shown. A miniaturized heparin extraction device comprises: a beating box 1;

[0032] A cutting pump 2, wherein the cutting pump 2 is arranged inside the beating box 1, a hydraulic pipe 3 is fixedly connected to one side of the cutting pump 2, a hand valve 14 is arranged at the bottom of one side of the outer surface of the beating box 1, a return pipe 13 is arranged on one side inside the beating box 1, a self-priming pump 5 is fixedly connected to the other end of the return pipe 13, and an enzymolysis tank 6 is fixedly connected to the other end of the hydraulic pipe 3;

[0033] A three-way pipe 11, the three-way pipe 11 is arranged on one side of the top of the enzymolysis tank 6, one end of the three-way pipe 11 is fixedly connected to an adsorption column 9, one side of the outer surface of the adsorption column 9 is fixedly installed with a drain valve 10, one side of the adsorption column 9 is connected to a high-efficiency filter column 8 through a pipeline, and one side of the high-efficiency filter column 8 is connected to a low-efficiency filter column 7 through a pipeline;

[0034] The heating pipe 15 is arranged at the bottom of the beating box 1 .

[0035] A stirring motor 12 is fixedly installed in the middle of the top of the enzymolysis tank 6, and a stirrer is fixedly connected to the bottom end of the output shaft of the stirring motor 12.

[0036] The number of the heating tubes 15 is three.

[0037] A discharge valve 4 is provided at the bottom of the beating box 1, and the hand valve 14 is connected to the beating box 1 and the enzymolysis tank 6 through a pipeline.

[0038] A mounting rod 193 is installed, and a slider 194 is fixedly installed at the bottom of one side of the connecting rod 191 .

[0039] The working principle of a miniaturized heparin extraction device provided by the utility model is as follows:

[0040] During operation, firstly, some soft water is injected into the pulping box 1, and then the thawed pig small intestine is poured into the pulping box 1, and the cutting pump 2 is started. The cutting pump 2 breaks the pig small intestine and then draws it into the enzymatic hydrolysis tank 6, and the valve connecting the enzymatic hydrolysis tank 6 and the pulping box 1 is opened, so that the liquid in the enzymatic hydrolysis tank 6 flows back to the pulping box 1 again, thereby forming a circular pulping process. After observing that the small intestine is broken to a certain extent, it can be stopped, and the connecting valve is closed, and all the solution in the pulping box 1 is pumped into the enzymatic hydrolysis tank 6 to enter the next process.

[0041] Turn on the stirring motor 12 of the enzymolysis tank 6 to drive the blades of the stirrer to rotate so that the liquid in the tank is always in motion, turn on the two heating tubes 15, start heating the liquid, and at the same time add an appropriate amount of alkaline water to the enzymolysis tank 6. After the temperature rises to 35°C, the pH is controlled between 8 and 9.

[0042] After the temperature reaches 35°C, add protease and lipase, stir evenly, and turn on the third heating tube 15 to raise the temperature to 58°C. During the temperature reaching 58°C, test the pH every 0.5h (the pH cannot be lower than 8). Stop heating when the temperature reaches 58°C and perform enzymatic hydrolysis for 3h until the small intestine is hydrolyzed into the shape of water (enzymatic hydrolysis is complete when there are no lumps); start raising the temperature to 83°C 4h after enzymatic hydrolysis, stir at intervals, stop heating when the temperature reaches 83°C, add industrial salt, and start filtering.

[0043] When filtering, load a 100-mesh filter bag into the first time, turn on the self-priming, 5, and let the liquid in the enzymolysis tank 6 flow into the pulping box 1 after passing through the filter bag. At the same time, turn on the cutting pump 2 to pump the liquid into the enzymolysis tank 6 to form a circulating filtration effect. Similarly, use 200-mesh and 300-mesh filters for fine filtration. After filtration, the pH is controlled between 8.5 and 9.

[0044] After filtration, the adsorption process is entered, the resin is added to the adsorption column 9, and the valve switches are checked to be normal, and the pressure relief valve is kept flowing uniformly. After the liquid temperature drops to 65°C, the stirring motor 12 of the enzymolysis tank 6 is turned on, and the valve direction of the self-priming pump 5 is switched to start cyclic adsorption. After the adsorption is completed, the resin is taken out, washed with warm water, and then placed in a salt solution for elution. After the elution is completed, 95% concentration alcohol is added to the liquid for precipitation. After the heparin sodium slurry is drained, it is dried and crushed, and packaged and stored to obtain the crude heparin.

[0045] Compared with the related art, the miniaturized heparin extraction device provided by the utility model has the following advantages:

[0046] Beneficial effects:

[0047] The utility model provides a miniaturized heparin extraction device. After the pig intestine is poured into a pulping box 1 through a stainless steel rotary cutting pump 2, a cutting and extraction method is adopted to cooperate with an enzymolysis tank 6 for a cycle to form a cyclic pulping process, thereby solving the problems of high cost and large floor space of a traditional crusher. A low-efficiency filtration + high-efficiency filtration + resin adsorption through-linked production mode is adopted, and the small intestine solution after enzymolysis is directly adsorbed by the resin in one process. At the same time, the method is cyclically adsorbed and settled to the bottom to extract all the heparin in the small intestine of the small batch produced pig, thereby realizing a complete process mode of output at the same time when it is put into production. The equipment is miniaturized, and production can be carried out anytime and anywhere as long as it is powered on and water is supplied. The single-machine full-process production mode realizes output at the same time when input is made. All structural installations of the equipment are installed in a quick-connect manner, which is convenient for installation, disassembly and maintenance.

[0048] Second embodiment

[0049] Please refer to Figure 3 , Figure 4 and Figure 5 Based on the miniaturized heparin extraction device provided in the first embodiment of the present application, the second embodiment of the present application provides another miniaturized heparin extraction device. The second embodiment is only a preferred embodiment of the first embodiment, and the implementation of the second embodiment will not affect the independent implementation of the first embodiment.

[0050] Specifically, the second embodiment of the present application provides a miniaturized heparin extraction device that is different in that, in the miniaturized heparin extraction device, a slide groove 16 is opened in the middle of one side of the outer surface of the pulping box 1, a slide rod 17 is fixedly installed inside the slide groove 16, an elastic member 18 is sleeved on the bottom of the outer surface of the slide rod 17, a moving device 19 is sleeved on the top of the outer surface of the slide rod 17, a cleaning device 20 is fixedly installed on one side of the bottom of the moving device 19, and a mounting frame 21 is fixedly installed in the middle of the top of the pulping box 1.

[0051] The cleaning device 20 comprises a moving box 201 , a cleaning block 203 is fixedly mounted on the bottom of the moving box 201 , and a connecting groove 202 is opened in the middle of the top of the moving box 201 .

[0052] The moving device 19 includes a connecting rod 191 , a fixing rod 192 is fixedly mounted on the top of one end of the connecting rod 191 , and the bottom of one side of the fixing rod 192 is fixed.

[0053] The elastic member 18 is a spring device, and the restoring elastic force of the elastic member 18 pushes the slider 194 to move upward inside the slide slot 16 .

[0054] The working principle of a miniaturized heparin extraction device provided by the utility model is as follows:

[0055] During operation, the user first pushes the fixing rod 192 downward, the fixing rod 192 drives the connecting rod 191 and the mounting rod 193 to move downward, the connecting rod 191 drives the slider 194 to slide downward on the surface of the slide rod 17 , and the slider 194 squeezes the elastic member 18 .

[0056] The mounting rod 193 drives the moving box 201 to move downward, and the moving box 201 drives the cleaning block 203 to move downward on the inner wall surface of the beating box 1, and the cleaning block 203 pushes the material adhering to the inner wall surface of the beating box 1 downward.

[0057] Compared with the related art, the miniaturized heparin extraction device provided by the utility model has the following advantages:

[0058] Beneficial effects:

[0059] The utility model provides a miniaturized heparin extraction device, which drives the cleaning device 20 to move downward along the inner wall surface of the pulping box 1 by pushing the moving device 19 downward, and pushes the residue adhering to the inner wall surface of the pulping box 1 downward, so as to prevent the residue from remaining on the inner wall surface of the pulping box 1. The device has a simple structure and strong utility model. When the material inside the pulping box 1 is guided into the enzymolysis tank 6 through the pump body, it is convenient to pump all the material inside the pulping box 1 into the enzymolysis tank 6, so as to prevent the material from remaining on the inner wall of the box, thereby improving the extraction efficiency and avoiding the waste of raw materials.

[0060] Second embodiment

[0061] Please refer to Figure 3 , Figure 4 and Figure 5 Based on the miniaturized heparin extraction device provided in the first embodiment of the present application, the second embodiment of the present application provides another miniaturized heparin extraction device. The second embodiment is only a preferred embodiment of the first embodiment, and the implementation of the second embodiment will not affect the independent implementation of the first embodiment.

[0062] Specifically, the second embodiment of the present application provides a miniaturized heparin extraction device, in which the output shaft of the stirring motor 12 is fixedly connected to a first stirring device 23 , and both sides of the first stirring device 23 are meshed with second stirring devices 24 .

[0063] The first stirring device 23 includes an active rod 231 , a first stirring plate 233 is fixedly mounted on the outer surface of the active rod 231 , and a driving gear 232 is fixedly mounted on the top of the outer surface of the active rod 231 .

[0064] The second stirring device 24 includes a rotating shaft 241 , a second stirring plate 243 is fixedly mounted on the outer surface of the rotating shaft 241 , and a driven gear 242 is fixedly mounted on the top of the outer surface of the rotating shaft 241 .

[0065] The active rod 231 and the rotating shaft 241 are both rotatably connected to the bottom of the stirring chamber 22, and the active gear 232 meshes with the driven gears 232 on the left and right sides to rotate. The active gear 232 and the two driven gears 232 rotate in opposite directions.

[0066] The first stirring plate 233 and the second stirring plate 243 are arranged in a staggered manner to avoid collision.

[0067] The working principle of a miniaturized heparin extraction device provided by the utility model is as follows:

[0068] During operation, first, the output shaft of the stirring motor 12 is controlled by an external power supply to rotate to drive the active rod 231 to rotate, and the active rod 231 rotates to drive the active gear 232 and the first stirring plate 233 to rotate, and the first stirring plate 233 rotates to stir the material inside the stirring chamber 22.

[0069] The driving gear 232 rotates to engage the driven gear 242, the driven gear 242 rotates to drive the rotating shaft 241 to rotate, the rotating shaft 241 rotates to drive the second stirring plate 243 to rotate, and the second stirring plate 243 rotates in the opposite direction to the first stirring plate 233 to stir, thereby improving the mixing effect.

[0070] Compared with the related art, the miniaturized heparin extraction device provided by the utility model has the following advantages:

[0071] Beneficial effects:

[0072] The utility model provides a miniaturized heparin extraction device, which controls the output shaft of a stirring motor 12 to rotate through an external power supply to drive a first stirring device 23 to rotate to stir the material inside an enzymolysis tank 6, and the first stirring device 23 engages with the second stirring device 24 to rotate, and at the same time stirs the material inside the enzymolysis tank 6. The device has a simple structure and strong utility model. The output shaft of the stirring motor 12 rotates to drive the first stirring device 23 and the second stirring device 24 to rotate together, thereby improving the stirring effect of the material inside the enzymolysis tank 6 and improving the mixing effect.

[0073] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A miniaturized heparin extraction device, characterized in that: include: Beater box; A cutting pump, wherein the cutting pump is arranged inside the beating box, a hydraulic pipe is fixedly connected to one side of the cutting pump, a hand valve is arranged at the bottom of one side of the outer surface of the beating box, a return pipe is arranged on one side inside the beating box, a self-priming pump is fixedly connected to the other end of the return pipe, and an enzymolysis tank is fixedly connected to the other end of the hydraulic pipe; A three-way pipe, wherein the three-way pipe is arranged on one side of the top of the enzymolysis tank, one end of the three-way pipe is fixedly connected to an adsorption column, one side of the outer surface of the adsorption column is fixedly installed with a sewage valve, one side of the adsorption column is connected to a high-efficiency filter column through a pipeline, and one side of the high-efficiency filter column is connected to a low-efficiency filter column through a pipeline; A heating tube is arranged at the bottom of the beating box.

2. A miniaturized heparin extraction device according to claim 1, characterized in that: A stirring motor is fixedly installed in the middle of the top of the enzymolysis tank, and a stirrer is fixedly connected to the bottom end of the output shaft of the stirring motor.

3. A miniaturized heparin extraction device according to claim 1, characterized in that: The number of the heating tubes is three.

4. A miniaturized heparin extraction device according to claim 1, characterized in that: A discharge valve is provided at the bottom of the beating box, and the hand valve is connected to the beating box and the enzymolysis tank through a pipeline.

5. A miniaturized heparin extraction device according to claim 1, characterized in that: A slide groove is provided in the middle of one side of the outer surface of the beating box, a slide rod is fixedly installed inside the slide groove, an elastic part is sleeved on the bottom of the outer surface of the slide rod, a moving device is sleeved on the top of the outer surface of the slide rod, a cleaning device is fixedly installed on one side of the bottom of the moving device, and a mounting frame is fixedly installed in the middle of the top of the beating box.

6. A miniaturized heparin extraction device according to claim 5, characterized in that: The cleaning device comprises a moving box, a cleaning block is fixedly mounted on the bottom of the moving box, and a connecting groove is opened in the middle of the top of the moving box.

7. A miniaturized heparin extraction device according to claim 5, characterized in that: The moving device comprises a connecting rod, a fixing rod is fixedly installed on the top of one end of the connecting rod, a mounting rod is fixedly installed on the bottom of one side of the fixing rod, and a sliding block is fixedly installed on the bottom of one side of the connecting rod.

8. A miniaturized heparin extraction device according to claim 2, characterized in that: The output shaft of the stirring motor is fixedly connected with a first stirring device, and both sides of the first stirring device are meshed with second stirring devices.

9. A miniaturized heparin extraction device according to claim 8, characterized in that: The first stirring device comprises an active rod, a first stirring plate is fixedly mounted on the outer surface of the active rod, and a driving gear is fixedly mounted on the top of the outer surface of the active rod.

10. A miniaturized heparin extraction device according to claim 8, characterized in that: The second stirring device comprises a rotating shaft, a second stirring plate is fixedly mounted on the outer surface of the rotating shaft, and a driven gear is fixedly mounted on the top of the outer surface of the rotating shaft.

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