Immune cell extraction device

Abstract

The utility model discloses an immune cell extraction device which comprises a fixed plate and a vertical frame, one side of the upper end of the fixed plate is in contact with an immune cell culture box, one side of the upper end of the vertical frame is fixedly connected with a driving motor, the output end of the driving motor is fixedly connected with a first screw rod, and the first screw rod is in threaded connection with a moving frame; one side of the moving frame is fixedly connected with a driving motor, the output end of the driving motor is fixedly connected with a second screw, the second screw is in threaded connection with a sliding block, the center of the upper end of the sliding block is fixedly connected with an air pump, the output end of the air pump is fixedly connected with a traction pipe, and the bottom end of the traction pipe is fixedly connected with a suction pipe; according to the immune cell culture box disclosed by the utility model, the infiltration box is arranged before the cell sap is adsorbed and is matched with the suction pipe capable of realizing high movement, the suction pipe is wetted, and the wetted cell sap enters the immune cell culture box for layered suction, so that the adsorption and overflow of the cell sap in the immune cell culture box are reduced, and the accuracy of data extraction is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of immune cell extraction, in particular to an immune cell extraction device. Background Art

[0002] After the immune cells are collected, they are placed in a test tube and subjected to density gradient centrifugation in a centrifuge to make the cells present different layers and achieve cell separation. At this time, they are extracted through test tubes or mechanical equipment to improve the extraction efficiency. However, it is difficult to control when using mechanical equipment for cell extraction.

[0003] According to the Chinese patent publication number CN217103874U, "An immune stem cell extraction device", it mainly describes that the immune stem cell sample in the immune stem cell culture box is sucked by controlling the lifting component to drive the suction component to move up and down, so as to suck the immune stem cell samples at different levels in the immune stem cell culture box. The operation is convenient and simple, and the precision is high. In the process of extracting cells with a pipette or other equipment, cell fluid adsorption and overflow will occur when the pipette moves in the test tube, resulting in inaccurate extraction data.

[0004] Therefore, an immune cell extraction device is proposed to solve the problem that in the process of extracting cells using a pipette or other equipment, cell fluid adsorption and overflow will occur when the pipette moves in the test tube, resulting in inaccurate extraction data. Utility Model Content

[0005] The technical problem to be solved by the utility model is that in the process of extracting cells with a pipette or other equipment, cell fluid adsorption and overflow may occur when the pipette moves in a test tube, resulting in inaccurate extraction data. Therefore, an immune cell extraction device is proposed.

[0006] The technical solution adopted by the utility model to solve the technical problem is: an immune cell extraction device comprises a fixed plate and a vertical frame, one side of the upper end of the fixed plate contacts with an immune cell culture box, one side of the upper end of the vertical frame is fixedly connected to a driving motor and the output end of the driving motor is fixedly connected to a first screw, a moving frame is threadedly connected to the first screw, one side of the moving frame is fixedly connected to the driving motor and the output end of the driving motor is fixedly connected to a second screw, a sliding block is threadedly connected to the second screw, an air pump is fixedly connected to the center of the upper end of the sliding block, the output end of the air pump is fixedly connected to a traction tube, the bottom end of the traction tube is fixedly connected to a suction tube, an infiltration box is fixedly connected to one side of the fixed plate, both sides of the upper end of the infiltration box are rotatably connected to sealing plates through a first rotating shaft, both ends of the first rotating shaft are fixedly connected to protrusions, and a torsion spring is sleeved on the first rotating shaft.

[0007] As a preferred technical solution of the utility model, both sides of the bottom end of the sliding block are fixedly connected with vertical rods, and the vertical rods are 5 cm lower than the traction tube. The straw can be protected by providing the vertical rods.

[0008] As a preferred technical solution of the utility model, a cushion block is fixedly connected to the bottom end of the vertical rod, and the cushion block is made of rubber material. The arrangement of the cushion block can reduce collision damage.

[0009] As a preferred technical solution of the utility model, one side of the suction tube is rotatably connected to a cover plate via a rotating shaft, a torsion spring is sleeved on the rotating shaft, and adsorption can be facilitated by providing a non-return structure.

[0010] As a preferred technical solution of the utility model, the upper end of the suction tube is fixedly connected with a cannula, one side of the cannula is plugged with a positioning rod, and the positioning rod is threadedly connected to the traction tube. The cannula and the positioning rod can be easily replaced.

[0011] The utility model has the following advantages: by setting an infiltration box before adsorbing the cell fluid, a highly movable suction tube can be realized, and the suction tube is moistened, and then enters the immune cell culture box for layered absorption after wetting, thereby reducing the adsorption and overflow of the cell fluid in the immune cell culture box and improving the accuracy of the extracted data. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 This is a schematic diagram of a side cross-sectional structure of an immune cell extraction device according to a preferred embodiment of the utility model;

[0013] Figure 2 This is a three-dimensional structural schematic diagram of an immune cell extraction device according to a preferred embodiment of the utility model;

[0014] Figure 3 It is a schematic diagram of the enlarged structure of position A of the immune cell extraction device in a preferred embodiment of the utility model.

[0015] Explanation of the reference numerals: 1. fixed plate; 2. vertical frame; 3. immune cell culture box; 4. first screw; 5. moving frame; 6. second screw; 7. slider; 8. air pump; 9. traction tube; 10. suction tube; 11. infiltration box; 12. first rotating shaft; 13. sealing plate; 14. vertical rod; 15. cushion block; 16. cover plate; 17. intubation tube; 18. positioning rod. DETAILED DESCRIPTION

[0016] The utility model will be further described below in conjunction with the accompanying drawings.

[0017] Please refer to Figure 1-3The immune cell extraction device shown includes a fixed plate 1 and a vertical frame 3. One side of the upper end of the fixed plate 1 contacts with an immune cell culture box 2, and the separated cell fluid layers are placed in the immune cell culture box 2. One side of the upper end of the vertical frame 3 is fixedly connected to a driving motor and the output end of the driving motor is fixedly connected to a first screw 4. A moving frame 5 is threadedly connected to the first screw 4. The moving frame 5 contacts the inner wall of the vertical frame 3. The driving motor drives the first screw 4 to rotate and makes the moving frame 5 move up and down. One side of the moving frame 5 is fixedly connected to the driving motor and the output end of the driving motor is fixedly connected to a second screw 6. A slider 7 is threadedly connected to the second screw 6. The driving motor drives the second screw 6 to rotate. The rod 6 rotates and causes the slider 7 to move in the moving frame 5. Both sides of the slider 7 contact the inner wall of the moving frame 5. An air pump 8 is fixedly connected to the center of the upper end of the slider 7. A traction tube 9 is fixedly connected to the output end of the air pump 8. A suction tube 10 is fixedly connected to the bottom end of the traction tube 9. The suction tube 10 is used to adsorb different layers in the cell fluid. An infiltration box 11 is fixedly connected to one side of the fixed plate 1. The infiltration box 11 is filled with cell fluid. Both sides of the upper end of the infiltration box 11 are rotatably connected to sealing plates 13 through a first rotating shaft 12. Both ends of the first rotating shaft 12 are fixedly connected to protrusions. A torsion spring is sleeved on the first rotating shaft 12. Both ends of the torsion spring are fixedly connected to the protrusion and the sealing plate 13 respectively.

[0018] The vertical rod 14 is fixedly connected to both sides of the bottom of the slider 7. The vertical rod 14 is 5 cm lower than the traction tube 9. By setting the vertical rod 14, the suction tube 10 can be protected when it moves downward to reduce collision damage.

[0019] The cushion block 15 is fixedly connected to the bottom end of the vertical rod 14 . The cushion block 15 is made of rubber material. By providing the cushion block 15 , the buffering effect is increased and the collision damage is reduced.

[0020] Among them, the cover plate 16 is rotatably connected to one side of the suction tube 10 through a rotating shaft, and a torsion spring is sleeved on the rotating shaft. The torsion spring is respectively fixedly connected to the cover plate 16 and the infiltration box 11. By setting the torsion spring and the rotating shaft, the cover plate 16 is driven to close the infiltration box 11 to achieve a dust-proof effect.

[0021] Among them, the traction tube 9 is threadedly connected to the positioning rod 18, and the positioning rod 18 is plugged with a cannula 17 (the cannula 17 has a built-in check valve, and the structure is consistent with the bottom side structure of the suction tube 10). The cannula 17 is fixedly connected to the upper end of the suction tube 10. By setting the positioning rod 18 to cooperate with the cannula 17, the suction tube 10 can be easily replaced.

[0022] Working principle: Start the driving motor on the vertical frame 3 to drive the first screw 4 to rotate, and make the moving frame 5 move in height, then the driving motor on the moving frame 5 drives the second screw 6 to rotate, and makes the slider 7 move, then the vertical rods 14 and pads 15 on both sides of the suction tube 10 unfold the sealing plate 13 downward, then the suction tube 10 is immersed in the infiltration box 11 and is soaked with the cell fluid, then the moving frame 5 is moved up, and the slider 7 is moved toward the immune cell culture box 2, at this time, when the suction tube 10 enters different levels in the immune cell culture box 2, select the appropriate level, start the air pump 8 and pull the suction tube 10 through the negative pressure of the traction tube 9, so that the cover 16 opens upward, and the cell fluid is pulled into the suction tube 10, thereby completing the extraction.

[0023] The above are only preferred implementations of the present invention. It should be pointed out that, for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

[0024] Other parts of the present invention not described in detail belong to the prior art and will not be described in detail here.

[0025] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solution deviate from the scope of the technical solution of the embodiments of the utility model.

Claims

1. An immune cell extraction device, comprising a fixed plate (1) and a vertical frame (3), wherein one side of the upper end of the fixed plate (1) contacts an immune cell culture box (2), characterized in that: One side of the upper end of the vertical frame (3) is fixedly connected to a driving motor, and the output end of the driving motor is fixedly connected to a first screw rod (4); a moving frame (5) is threadedly connected to the first screw rod (4); one side of the moving frame (5) is fixedly connected to a driving motor, and the output end of the driving motor is fixedly connected to a second screw rod (6); a sliding block (7) is threadedly connected to the second screw rod (6); an air pump (8) is fixedly connected to the center of the upper end of the sliding block (7); a traction tube (9) is fixedly connected to the output end of the air pump (8); a suction tube (10) is fixedly connected to the bottom end of the traction tube (9); an immersion box (11) is fixedly connected to one side of the fixed plate (1); both sides of the upper end of the immersion box (11) are rotatably connected to a sealing plate (13) via a first rotating shaft (12); protrusions are fixedly connected to both ends of the first rotating shaft (12); a torsion spring is sleeved on the first rotating shaft (12).

2. The immune cell extraction device according to claim 1, characterized in that: Both sides of the bottom end of the sliding block (7) are fixedly connected with vertical rods (14), and the vertical rods (14) are 5 cm lower than the traction tube (9).

3. The immune cell extraction device according to claim 2, characterized in that: A cushion block (15) is fixedly connected to the bottom end of the vertical rod (14), and the cushion block (15) is made of rubber material.

4. The immune cell extraction device according to claim 1, characterized in that: One side of the suction tube (10) is rotatably connected to a cover plate (16) via a rotating shaft, and a torsion spring is sleeved on the rotating shaft.

5. The immune cell extraction device according to claim 4, characterized in that: The upper end of the suction tube (10) is fixedly connected to a cannula (17), one side of the cannula (17) is plugged with a positioning rod (18), and the positioning rod (18) is threadedly connected to the traction tube (9).

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