Cell active oxygen detection device
By using a combination of rotating shaft, gear, rotating chamber, tooth rod and servo motor in the cell reactive oxygen detection device, the uniform movement and angle adjustment of the detection needle are achieved, which solves the positioning difficulties in the prior art and the detection needle can only move horizontally. The problem improves the accuracy and stability of the detection.
Patent Information
- Application Number
- CN202420911952.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-29
- Publication Date
- 2024-12-27
- Estimated Expiration
- 2034-04-29
AI Technical Summary
Existing cell reactive oxygen detection devices are difficult to accurately locate cells, and the detection needle can only move sideways and cannot effectively pierce the cells.
A cell reactive oxygen detection device including a base plate, a connecting rotor, a top plate, an observation cylinder, an eyepiece cylinder, a detection assembly and a servo motor is designed. Through a combination of a rotating shaft, a gear, a rotating chamber, a tooth rod, a detection needle and a servo motor, Achieve constant speed movement and angle adjustment of the detection needle to ensure stable puncture.
The device can better pierce the inside of the cell, improve the accuracy and stability of the detection, and facilitate observation and puncture by fine-tuning the position of the Petri dish.
Smart Images
Figure CN222250780U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a cell active oxygen detection device. Background Art
[0002] Reactive oxygen refers to the general term for oxygen-containing free radicals and peroxides that are easy to form free radicals and are related to oxygen metabolism in the body. In the process of detecting cellular reactive oxygen, it is necessary to use fluorescent probes to puncture the inside of the cell for detection, so that the amount of reactive oxygen inside the cell can be observed;
[0003] The existing authorization publication number is "CN218766624U" and a cell active oxygen detection device, which includes a base, a fixing mechanism is provided on the base, and the fixing mechanism includes a placement hole and a sliding rod. The top of the base is provided with the placement hole, and the three sliding rods are slidably connected to the inner wall of the placement hole. A control mechanism is provided inside the base, and the control mechanism includes a rotating shaft, a first rotating rod, a second rotating rod, a first sprocket, a second sprocket, a chain, a worm and a worm wheel. The rotating shaft is rotatably connected to one side of the base, and the worm is fixedly connected to one end of the rotating shaft located inside the base. The first rotating rod and the second rotating rod are rotatably connected to the bottom inner wall of the base, and the first sprocket and the second sprocket are respectively fixedly connected to the first rotating rod and the second rotating rod, which can fix the culture dish for culturing cells and facilitate the movement of the fluorescent probe.
[0004] However, cells are very small structures and cannot be directly observed by the naked eye. The device directly uses a probe for puncture, but it is not possible to find the cells well, which limits the detection. In addition, the probe can only move laterally, so it is not possible to puncture the cells well. Utility Model Content
[0005] The purpose of the utility model is to provide a cell active oxygen detection device to solve the existing problems raised in the above background technology.
[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell active oxygen detection device, comprising a bottom plate, a connecting drum is provided on the top of the bottom plate, a top plate is provided on the top of the connecting drum, a plurality of adjustment members are provided at one end of the top plate, an observation tube is provided on one side of the top of the top plate, an eyepiece tube is provided at the bottom of the observation tube, a mounting bin is provided on the outside of the eyepiece tube, a connecting groove is provided at the bottom of the mounting bin, a detection component is provided inside the mounting bin, a placement groove is provided on one side of the top of the bottom plate, a second connecting bin is provided at the bottom of the placement groove, a fourth servo motor is provided on one side of the second connecting bin, a second screw rod is provided inside the fourth servo motor, an output end of the second screw rod is connected to the fourth servo motor, a first connecting bin is provided on the top of the second screw rod, a third servo motor is provided at one end of the first connecting bin, a first screw rod is provided inside the first connecting bin, the first screw rod is connected to the output end of the third servo motor, and a culture dish placement plate is provided on the top of the first screw rod.
[0007] Preferably, the detection assembly includes a rotating shaft, a gear, a rotating chamber, a gear rod, a detection needle, a connecting rod, a first servo motor and a second servo motor. The installation chamber is provided with a rotating chamber inside, a rotating shaft is provided at one end of the rotating chamber, a gear is provided on the outside of the rotating shaft, a detection needle is provided at the bottom inside the rotating chamber, a gear rod is provided on the top of the detection needle, a first servo motor is provided at one end of the rotating chamber, an output end of the first servo motor is connected to the rotating shaft, a connecting rod is provided at one end of the rotating chamber away from the first servo motor, a second servo motor is provided at one end of the installation chamber, and an output end of the second servo motor is connected to the connecting rod. During the detection process, the detection needle can be controlled to move at a uniform speed, so that the detection needle can better puncture into the interior of the cell to ensure the stability of the puncture. At the same time, during the puncture process, the puncture angle can also be adjusted, thereby facilitating the adjustment of the position of the detection needle.
[0008] The first connecting chamber and the second connecting chamber are both provided with through grooves, and the second screw rod and the first screw rod are both located inside the through grooves, so that the second screw rod and the first screw rod can be connected to the first connecting chamber and the culture dish placement plate respectively.
[0009] The rotating bin is provided with a through hole, the detection needle is located inside the through hole, a guide tube is provided at the bottom of the rotating bin, and the guide tube is communicated with the through hole, so that the detection needle can extend to the outside through the through hole, so that the detection needle can puncture cells.
[0010] An observation port is arranged at the bottom of the installation chamber, and the bottom of the eyepiece tube is located inside the observation port, so that the eyepiece tube can observe cells through the observation port.
[0011] A socket is provided at the bottom of the gear rod, and the top of the detection needle is located inside the socket, so that the gear rod can be connected to the detection needle and the detection needle can be easily disassembled.
[0012] Compared with the prior art, the utility model has the following beneficial effects: the cell active oxygen detection device;
[0013] Through the cooperation of the first connecting chamber, the third servo motor, the first screw rod, the second screw rod, the culture dish placement plate, the fourth servo motor and the second connecting chamber, during the process of cell active oxygen detection, the position of the cell culture dish can be fine-tuned so that the culture dish can be adjusted to the observation position of the eyepiece tube and puncture is convenient at the same time. Then, through the cooperation of the rotating shaft, gears, rotating chamber, gear rod, detection needle, connecting rod, the first servo motor and the second servo motor, during the detection process, the detection needle can be controlled to move at a uniform speed so that the detection needle can better puncture into the interior of the cell and ensure the stability of the puncture. At the same time, during the puncture process, the puncture angle can also be adjusted, thereby facilitating the adjustment of the position of the detection needle. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 It is a three-dimensional diagram of the utility model;
[0015] Figure 2 This is a front cross-sectional view of the utility model;
[0016] Figure 3 It is a top view of the first connecting compartment of the utility model;
[0017] Figure 4 This is a structural diagram of the detection component of the utility model;
[0018] Figure 5 It is a top view of the rotating bin of the utility model;
[0019] Figure 6 It is a top view of the installation bin of the utility model.
[0020] In the figure: 1, bottom plate; 2, connecting drum; 3, top plate; 4, adjusting part; 5, observation tube; 6, installation chamber; 7, detection assembly; 71, rotating shaft; 72, gear; 73, rotating chamber; 74, gear rod; 75, detection needle; 76, connecting rod; 77, first servo motor; 78, second servo motor; 8, connecting groove; 9, placement groove; 10, eyepiece tube; 11, first connecting chamber; 12, third servo motor; 13, first screw rod; 14, second screw rod; 15, culture dish placement plate; 16, fourth servo motor; 17, second connecting chamber. DETAILED DESCRIPTION
[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0022] See also Figure 1-Figure 6 The utility model provides an embodiment: a cell active oxygen detection device, comprising a bottom plate 1, a connecting drum 2 is provided on the top of the bottom plate 1, a top plate 3 is provided on the top of the connecting drum 2, a plurality of adjustment members 4 are provided at one end of the top plate 3, an observation tube 5 is provided on one side of the top of the top plate 3, an eyepiece tube 10 is provided at the bottom of the observation tube 5, a mounting chamber 6 is provided on the outside of the eyepiece tube 10, an observation port is provided at the bottom of the mounting chamber 6, the bottom of the eyepiece tube 10 is located inside the observation port, so that the eyepiece tube 10 can observe cells through the observation port, a connecting groove 8 is provided at the bottom of the mounting chamber 6, and a detection component 7 is provided inside the mounting chamber 6;
[0023] The detection assembly 7 includes a rotating shaft 71, a gear 72, a rotating chamber 73, a gear rod 74, a detection needle 75, a connecting rod 76, a first servo motor 77 and a second servo motor 78. The rotating chamber 73 is provided inside the installation chamber 6. A rotating shaft 71 is provided at one end of the rotating chamber 73. A gear 72 is provided on the outer side of the rotating shaft 71. A detection needle 75 is provided at the bottom of the rotating chamber 73. A gear rod 74 is provided at the top of the detection needle 75. A plug hole is provided at the bottom of the gear rod 74. The top of the detection needle 75 is located inside the plug hole, so that the gear rod 74 can be connected to the detection needle 75 and the detection needle 75 can be easily disassembled. A through hole is provided at the rotating chamber 73, and the detection needle 75 is located inside the through hole.
[0024] A guide tube is provided at the bottom of the rotating chamber 73, and the guide tube is communicated with the through hole, so that the detection needle 75 can extend to the outside through the through hole, so that the detection needle 75 can puncture the cell. A first servo motor 77 is provided at one end of the rotating chamber 73, and the output end of the first servo motor 77 is interconnected with the rotating shaft 71. A connecting rod 76 is provided at one end of the rotating chamber 73 away from the first servo motor 77, and a second servo motor 78 is provided at one end of the mounting chamber 6, and the output end of the second servo motor 78 is interconnected with the connecting rod 76. During the detection process, the detection needle 75 can be controlled to move at a uniform speed, so that the detection needle 75 can better puncture the inside of the cell to ensure the stability of the puncture. At the same time, during the puncture process, the puncture angle can also be adjusted, thereby facilitating the adjustment of the position of the detection needle 75.
[0025] A placement groove 9 is provided on one side of the top of the bottom plate 1, a second connecting chamber 17 is provided at the bottom inside the placement groove 9, a fourth servo motor 16 is provided on one side of the second connecting chamber 17, a second screw rod 14 is provided inside the fourth servo motor 16, the output end of the second screw rod 14 is connected to the fourth servo motor 16, a first connecting chamber 11 is provided on the top of the second screw rod 14, a third servo motor 12 is provided at one end of the first connecting chamber 11, a first screw rod 13 is provided inside the first connecting chamber 11, the first screw rod 13 is connected to the output end of the third servo motor 12, a culture dish placement plate 15 is provided on the top of the first screw rod 13, through grooves are provided inside the first connecting chamber 11 and the second connecting chamber 17, the second screw rod 14 and the first screw rod 13 are both located inside the through grooves, so that the second screw rod 14 and the first screw rod 13 can be connected to the first connecting chamber 11 and the culture dish placement plate 15 respectively.
[0026] Working principle: During the detection process, first place the culture dish on the top of the culture dish placement plate 15, then adjust the observation tube 5 to focus through the adjustment member 4, and then start the third servo motor 12 and the fourth servo motor 16, and then drive the second screw rod 14 and the first screw rod 13 to rotate during the startup process. During the rotation process, the second screw rod 14 drives the first connecting bin 11 to move, and then the first screw rod 13 drives the culture dish placement plate 15 to move, thereby adjusting the position of the culture dish placement plate 15 so that the culture dish on the culture dish placement plate 15 can be aligned with the bottom of the eyepiece tube 10. At this time, start the second servo motor 78, and the second servo motor 78 drives the connecting rod 76 and the rotating bin 73 to rotate. When the rotating bin 73 is adjusted to a suitable position, start the first servo motor 77, and then the first servo motor 77 drives the rotating shaft 71 and the gear 72 to rotate. During the rotation process, the gear 72 drives the gear rod 74 and the detection needle 75 to move, and observes through the observation tube 5 and the eyepiece tube 10, and then the detection needle 75 punctures the cells to detect the cells.
[0027] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.
[0028] In the description of the present utility model, unless otherwise specified, "multiple" means two or more than two; the orientation or position relationship indicated by the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail", etc. is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present utility model. In addition, the terms "first", "second", "third", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance. In the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connected" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.
Claims
1. A cell active oxygen detection device, comprising a bottom plate (1), characterized in that: A connecting drum (2) is provided on the top of the bottom plate (1), a top plate (3) is provided on the top of the connecting drum (2), a plurality of adjustment members (4) are provided at one end of the top plate (3), an observation tube (5) is provided on one side of the top of the top plate (3), an eyepiece tube (10) is provided on the bottom of the observation tube (5), a mounting chamber (6) is provided on the outside of the eyepiece tube (10), a connecting groove (8) is provided on the bottom of the mounting chamber (6), a detection assembly (7) is provided inside the mounting chamber (6), a placement groove (9) is provided on one side of the top of the bottom plate (1), and a second connection chamber (17) is provided at the bottom of the placement groove (9). A fourth servo motor (16) is provided on one side of the second connecting chamber (17); a second screw rod (14) is provided inside the fourth servo motor (16); an output end of the second screw rod (14) is connected to the fourth servo motor (16); a first connecting chamber (11) is provided on the top of the second screw rod (14); a third servo motor (12) is provided at one end of the first connecting chamber (11); a first screw rod (13) is provided inside the first connecting chamber (11); the first screw rod (13) is connected to the output end of the third servo motor (12); and a culture dish placement plate (15) is provided on the top of the first screw rod (13).
2. A cell active oxygen detection device according to claim 1, characterized in that: The detection assembly (7) comprises a rotating shaft (71), a gear (72), a rotating chamber (73), a gear rod (74), a detection needle (75), a connecting rod (76), a first servo motor (77) and a second servo motor (78). The mounting chamber (6) is provided with a rotating chamber (73) inside. A rotating shaft (71) is provided at one end of the rotating chamber (73). A gear (72) is provided on the outside of the rotating shaft (71). A detection needle (75) is provided at the bottom of the rotating chamber (73). A gear rod (74) is provided on the top of the detection needle (75). A first servo motor (77) is provided at one end of the rotating chamber (73). An output end of the first servo motor (77) is connected to the rotating shaft (71). A connecting rod (76) is provided at one end of the rotating chamber (73) away from the first servo motor (77). A second servo motor (78) is provided at one end of the mounting chamber (6). An output end of the second servo motor (78) is connected to the connecting rod (76).
3. A cell active oxygen detection device according to claim 1, characterized in that: The first connecting chamber (11) and the second connecting chamber (17) are both provided with through slots, and the second screw rod (14) and the first screw rod (13) are both located inside the through slots.
4. A cell active oxygen detection device according to claim 2, characterized in that: The rotating bin (73) is provided with a through hole, the detection needle (75) is located inside the through hole, and a guide tube is provided at the bottom of the rotating bin (73), and the guide tube is in communication with the through hole.
5. A cell active oxygen detection device according to claim 1, characterized in that: An observation port is provided at the bottom of the installation chamber (6), and the bottom of the eyepiece barrel (10) is located inside the observation port.
6. A cell active oxygen detection device according to claim 2, characterized in that: The bottom of the gear rod (74) is provided with an insertion hole, and the top of the detection needle (75) is located inside the insertion hole.