NK cell culture medium preparation device
The NK cell culture medium preparation device addresses uneven mixing by incorporating a baffle mechanism, achieving uniform cell mixing and stability through increased resistance during the stirring process.
Patent Information
- Application Number
- CN202420554609.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-21
- Publication Date
- 2024-12-31
- Estimated Expiration
- 2034-03-21
AI Technical Summary
Existing NK cell culture medium preparation devices lack a baffle in their stirring blades, leading to uneven mixing during the preparation process.
A NK cell culture medium preparation device with a baffle mechanism, comprising a rotating shaft, fixed plate, fixing bolt, limit groove, and baffle, which ensures uniform stirring by increasing resistance and promoting full mixing of cells.
The device achieves uniform cell mixing by using a baffle mechanism that stabilizes the baffle installation and enhances stirring uniformity, ensuring thorough cell dispersion.
Smart Images
Figure CN222266822U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field related to cell culture, in particular to a NK cell culture medium preparation device. Background Art
[0002] Natural killer cells (NK cells) are a type of lymphocyte with natural killing ability. Their main function is to identify and eliminate damaged cells, virus-infected cells, tumor cells and other abnormal cells in the body. NK cell culture refers to the proliferation and differentiation of NK cells through specific culture methods under laboratory conditions, thereby obtaining a large number of highly active NK cells for research and treatment. Therefore, a NK cell culture medium preparation device is particularly needed.
[0003] However, the existing NK cell culture medium preparation device needs to be stirred during the preparation process, but the stirring blade of the general preparation device is not equipped with a baffle, resulting in uneven stirring during the stirring process, thereby causing uneven mixing between cells. Utility Model Content
[0004] The purpose of the utility model is to provide a NK cell culture medium preparation device to solve the problem that the existing NK cell culture medium preparation device proposed in the above-mentioned background technology needs to be stirred during the preparation process, but the stirring blade of the general preparation device is not equipped with a baffle, resulting in uneven stirring during the stirring process, thereby causing the problem that the cells cannot be evenly mixed.
[0005] To achieve the above-mentioned object, the utility model provides the following technical solutions: an NK cell culture medium preparation device, comprising a stirring barrel, an observation mechanism is arranged on the surface of the stirring barrel, a base is fixedly connected to the bottom of the stirring barrel, a barrel cover is threadedly connected to the surface of the stirring barrel, a material injection port is opened on the surface of the barrel cover, a stirring mechanism is arranged on the surface of the barrel cover, the stirring mechanism, a second stirring blade is arranged at the bottom of the stirring mechanism, and a baffle mechanism is arranged on the surface of the second stirring blade;
[0006] The baffle mechanism includes a rotating shaft, a fixed plate, a fixing bolt, a limit groove, a limit block and a baffle. The surface of the second stirring blade is fixedly connected to the rotating shaft, the surface of the rotating shaft is rotatably connected to the fixed plate, the surface of the fixed plate is penetrated by a fixing bolt, the surface of the second stirring blade is provided with a limit groove, the interior of the limit groove is connected to a limit block, and the surface of the limit block is fixedly connected to the baffle.
[0007] Preferably, the fixed plate and the second stirring blade form a rotating structure through a rotating shaft, and the size of the limiting groove matches the size of the limiting block.
[0008] Preferably, the observation mechanism comprises a slot, a scale plate and scale lines. The surface of the mixing barrel is provided with a slot, the interior of the slot is fixedly connected with a scale plate, and the surface of the scale plate is provided with scale lines.
[0009] Preferably, the stirring mechanism includes a motor, a motor shaft, a first stirring blade and a brush head. The motor is fixedly mounted on the surface of the barrel cover, one end of the motor is fixedly connected to the motor shaft, the surface of the motor shaft is fixedly connected to the first stirring blade, one end of the first stirring blade is fixedly connected to the brush head, and the surface of the motor shaft is fixedly connected to the second stirring blade.
[0010] Preferably, the first stirring blade forms a rotating structure with the motor through the motor shaft, and the brush heads are arranged in multiple groups on the surface of the first stirring blade.
[0011] Compared with the prior art, the beneficial effect of the utility model is as follows: the NK cell culture medium preparation device, through the setting of the baffle mechanism, before stirring, first put the baffle into the limit groove, then rotate the fixed plate to the surface of the limit block through the rotating shaft, and then screw on the fixing bolt, and then inject the cells to be stirred into the stirring barrel through the injection port. At this time, during the stirring process, the baffle will increase the resistance to make the stirring more uniform, so that the cells are fully mixed. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] Figure 1 This is a schematic diagram of the side view of the appearance structure of the utility model;
[0013] Figure 2 This is a schematic diagram of the cross-sectional structure of the stirring mechanism of the utility model;
[0014] Figure 3 This is a schematic cross-sectional view of the baffle mechanism of the utility model;
[0015] Figure 4 For this utility model Figure 3 Enlarged structural diagram at A in the middle.
[0016] In the figure: 1. mixing barrel; 2. observation mechanism; 201. slot; 202. scale plate; 203. scale line; 3. base; 4. barrel cover; 5. filling port; 6. mixing mechanism; 601. motor; 602. motor shaft; 603. first mixing blade; 604. brush head; 7. second mixing blade; 8. baffle mechanism; 801. rotating shaft; 802. fixing plate; 803. fixing bolt; 804. limiting groove; 805. limiting block; 806. baffle. DETAILED DESCRIPTION
[0017] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0018] See also Figure 1-4 The utility model provides a technical solution: a NK cell culture medium preparation device, comprising a stirring barrel 1, an observation mechanism 2 is arranged on the surface of the stirring barrel 1, a base 3 is fixedly connected to the bottom of the stirring barrel 1, a barrel cover 4 is threadedly connected to the surface of the stirring barrel 1, a material injection port 5 is opened on the surface of the barrel cover 4, a stirring mechanism 6 is arranged on the surface of the barrel cover 4, a second stirring blade 7 is arranged at the bottom of the stirring mechanism 6, and a baffle mechanism 8 is arranged on the surface of the second stirring blade 7;
[0019] The baffle mechanism 8 includes a rotating shaft 801, a fixed plate 802, a fixing bolt 803, a limiting groove 804, a limiting block 805 and a baffle 806. The surface of the second stirring blade 7 is fixedly connected to the rotating shaft 801, the surface of the rotating shaft 801 is rotatably connected to the fixed plate 802, the surface of the fixed plate 802 is penetrated by the fixing bolt 803, the surface of the second stirring blade 7 is provided with a limiting groove 804, the interior of the limiting groove 804 is connected to the limiting block 805, and the surface of the limiting block 805 is fixedly connected to the baffle 806. Through the setting of the baffle mechanism 8, before stirring, the baffle 806 is first placed in the limiting groove 804, and then the fixed plate 802 is rotated to the surface of the limiting block 805 through the rotating shaft 801, and then the fixing bolt 803 is screwed on, and then the cells to be stirred are injected into the stirring barrel 1 through the injection port 5. At this time, during the stirring process, the baffle 806 will increase the resistance to make the stirring more uniform, thereby fully mixing the cells.
[0020] Furthermore, the fixed plate 802 forms a rotating structure with the second stirring blade 7 through the rotating shaft 801, and the size of the limiting groove 804 is consistent with the size of the limiting block 805. Through the setting of the limiting groove 804 and the limiting block 805, when in use, the limiting block 805 is connected to the limiting groove 804 and limits the baffle 806 at the same time, thereby making the installation of the baffle 806 more stable.
[0021] Furthermore, the observation mechanism 2 includes a slot 201, a scale plate 202 and scale lines 203. The slot 201 is opened on the surface of the mixing barrel 1, and the scale plate 202 is fixedly connected to the inside of the slot 201. The surface of the scale plate 202 is provided with scale lines 203. Through the setting of the scale plate 202 and the scale lines 203, during the stirring process, the dosage of the cells inside the mixing barrel 1 can be judged by observing the scale lines 203 on the surface of the scale plate 202.
[0022] Furthermore, the stirring mechanism 6 includes a motor 601, a motor shaft 602, a first stirring blade 603 and a brush head 604. The motor 601 is fixedly installed on the surface of the barrel cover 4, one end of the motor 601 is fixedly connected to the motor shaft 602, the surface of the motor shaft 602 is fixedly connected to the first stirring blade 603, one end of the first stirring blade 603 is fixedly connected to the brush head 604, and the surface of the motor shaft 602 is fixedly connected to the second stirring blade 7. Through the setting of the stirring mechanism 6, when stirring, the motor 601 is started first. The operation of the motor 601 will drive the first stirring blade 603 and the second stirring blade 7 to start rotating through the motor shaft 602 to stir the cells in the stirring barrel 1. At the same time, the brush head 604 will clean the inside of the stirring barrel 1 to avoid cells remaining on the inner wall surface of the stirring barrel 1.
[0023] Furthermore, the first stirring blade 603 forms a rotating structure with the motor 601 through the motor shaft 602, and multiple groups of brush heads 604 are arranged on the surface of the first stirring blade 603. Through the arrangement of the brush heads 604, when in use, multiple groups of brush heads 604 will make the cleaning of the inner wall of the stirring barrel 1 more efficient.
[0024] Working principle: Before stirring, first place the baffle 806 into the limit groove 804, then rotate the fixing plate 802 to the surface of the limit block 805 through the rotating shaft 801, and then screw on the fixing bolt 803, and then inject the cells to be stirred into the stirring barrel 1 through the injection port 5. At this time, during the stirring process, the baffle 806 will increase the resistance to make the stirring more uniform, so that the cells are fully mixed. When stirring, first start the motor 601. The operation of the motor 601 will drive the first stirring blade 603 and the second stirring blade 7 to start rotating through the motor shaft 602 to stir the cells in the stirring barrel 1. At the same time, the brush head 604 will clean the inside of the stirring barrel 1 to avoid cells remaining on the inner wall surface of the stirring barrel 1. In addition, during the stirring process, the dosage of the cells inside the stirring barrel 1 can be judged by observing the scale line 203 on the surface of the scale plate 202.
[0025] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A NK cell culture medium preparation device, comprising a stirring barrel (1), characterized in that: The surface of the mixing barrel (1) is provided with an observation mechanism (2); the bottom of the mixing barrel (1) is fixedly connected with a base (3); the surface of the mixing barrel (1) is threadedly connected with a barrel cover (4); the surface of the barrel cover (4) is provided with a material injection port (5); the surface of the barrel cover (4) is provided with a stirring mechanism (6); the stirring mechanism (6) and the stirring mechanism (6) are provided with a second stirring blade (7) at the bottom; and the surface of the second stirring blade (7) is provided with a baffle mechanism (8); The baffle mechanism (8) comprises a rotating shaft (801), a fixing plate (802), a fixing bolt (803), a limiting groove (804), a limiting block (805) and a baffle (806); the surface of the second stirring blade (7) is fixedly connected to the rotating shaft (801); the surface of the rotating shaft (801) is rotatably connected to the fixing plate (802); the surface of the fixing plate (802) is penetrated by the fixing bolt (803); the surface of the second stirring blade (7) is provided with a limiting groove (804); the limiting block (805) is connected inside the limiting groove (804); and the surface of the limiting block (805) is fixedly connected to the baffle (806).
2. A NK cell culture medium preparation device according to claim 1, characterized in that: The fixed plate (802) forms a rotating structure with the second stirring blade (7) via the rotating shaft (801), and the size of the limiting groove (804) matches the size of the limiting block (805).
3. A NK cell culture medium preparation device according to claim 1, characterized in that: The observation mechanism (2) comprises a slot (201), a scale plate (202) and scale lines (203); the surface of the mixing barrel (1) is provided with a slot (201); the interior of the slot (201) is fixedly connected with the scale plate (202); and the surface of the scale plate (202) is provided with scale lines (203).
4. The NK cell culture medium preparation device according to claim 1, characterized in that: The stirring mechanism (6) comprises a motor (601), a motor shaft (602), a first stirring blade (603) and a brush head (604); the motor (601) is fixedly mounted on the surface of the barrel cover (4); one end of the motor (601) is fixedly connected to the motor shaft (602); the surface of the motor shaft (602) is fixedly connected to the first stirring blade (603); one end of the first stirring blade (603) is fixedly connected to the brush head (604); and the surface of the motor shaft (602) is fixedly connected to the second stirring blade (7).
5. A NK cell culture medium preparation device according to claim 4, characterized in that: The first stirring blade (603) forms a rotating structure with the motor (601) via the motor shaft (602), and the brush head (604) is provided in multiple groups on the surface of the first stirring blade (603).