Suspension cell culture bottle
By designing horizontally rotatable flexible rod and vertical rod structures in suspended cell culture flasks, the problems of poor stirring effect and bacterial contamination in the prior art are solved, and a more uniform cell dispersion and better cleaning effect are achieved.
Patent Information
- Application Number
- CN202323155637.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2023-11-22
- Publication Date
- 2024-12-31
- Estimated Expiration
- 2033-11-22
AI Technical Summary
The existing suspended cell culture flasks have poor agitation effect and are easily contaminated by bacteria, and there are blind spots during cleaning.
A suspended cell culture bottle consisting of a bottle body and a bottle cap is designed. The bottle cap is equipped with a vertical vertical pole and a flexible rod. The lower end of the pole extends into the bottle body. The flexible rod can be rotated horizontally for stirring and is in the bottle. Set a partition and disinfectant chamber inside the cover to avoid bacterial contamination.
The suspension cell culture is achieved with simple structure and good stirring effect, avoiding bacterial contamination, and simplifying the cleaning process.
Smart Images
Figure CN222274586U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of animal cell culture, in particular to a suspension cell culture bottle. Background Art
[0002] At present, animal cells are mostly cultured in cell culture bottles when they are cultured in vitro. At present, most cell culture bottles are flat transparent bottles, and the culture is static, which is conducive to the adherent culture of animal cells, but it is not suitable for culturing suspended cells. There are also culture bottles on the market that can stir and disperse cell clusters, but their structures are relatively complex, such as the cell culture bottle disclosed in document number CN217895635U "A Cell Culture Bottle", but its hose is vertically arranged and consistent with the axis of rotation, so the stirring performance of the hose is not good, and two stirring parts are provided, which increases the probability cost of bacterial contamination, and there are dead corners during cleaning. Utility Model Content
[0003] In order to solve the above technical problems, the purpose of the utility model is to provide a suspension cell culture bottle with a simple structure, good stirring effect and convenient tilting.
[0004] In order to achieve the above-mentioned purpose, the technical scheme of the utility model is as follows: a suspension cell culture bottle, which includes a bottle body and a bottle cap, a bottle mouth with an external thread is arranged at the middle part of the upper end of the bottle body, the bottle cap is a groove-shaped cover, and its groove faces downward, and an internal thread matching the bottle mouth is arranged on the inner side wall of the bottle cap, a vertically penetrating vertical rod is arranged in the middle part of the bottle cap and is sealingly and slidably connected with the vertical rod, a flexible rod is arranged at the lower end of the vertical rod, the bottle cap is threadedly connected with the bottle mouth, and the lower end of the vertical rod extends into the bottle body, the bottle body contains cell culture fluid, and the upper end of the vertical rod is rotated so that the cell culture fluid in the bottle body can be stirred by the flexible rod.
[0005] The beneficial effect of the above technical solution is that the bottle cap can be threadedly connected to the bottle mouth of the bottle body, and the lower end of the vertical rod extends into the bottle body, and the flexible rod is also stretched out in the bottle body. The vertical rod can be manually rotated to drive the flexible rod to rotate horizontally, and the vertical rod can also slide up and down relative to the bottle cap, so that different depths in the bottle body can be stirred.
[0006] In the above technical solution, the flexible rod is vertically connected to the vertical rod.
[0007] The beneficial effects of the above technical solution are: its structure is simple and the stirring effect is good.
[0008] In the above technical solution, the flexible rod is provided with one, and the plurality of the flexible rods are vertically spaced and distributed, and two adjacent flexible rods are cross-distributed.
[0009] The beneficial effect of the above technical solution is that the plurality of flexible rods can cooperate to stir the cell culture medium, thereby making the cells therein more evenly dispersed.
[0010] The upper end of the vertical pole in the above technical solution is also provided with a handle.
[0011] The beneficial effect of the above technical solution is that it makes it more convenient to manually rotate the vertical pole.
[0012] In the above technical solution, a partition is horizontally arranged inside the bottle cap, and the partition divides the bottle cap into a lower groove chamber and an upper chamber. The internal thread is arranged on the inner side wall of the lower groove chamber. The vertical rod passes through the upper end of the bottle cap and the partition. The upper chamber is used to contain disinfectant, which is used to disinfect the surface of the vertical rod.
[0013] The beneficial effect of the above technical solution is that it can prevent bacteria from entering the bottle body through the position where the vertical rod penetrates the bottle cap.
[0014] In the above technical solution, the upper end of the bottle cap is also provided with a filling port connected to the upper chamber, and the filling port is used to inject disinfectant into the upper chamber.
[0015] The beneficial effect of the above technical solution is that the disinfectant in the upper chamber can be added or replaced.
[0016] The filling port in the above technical solution is also embedded with a flexible plug.
[0017] The beneficial effect of the above technical solution is that the upper chamber can be sealed in this way, and disinfectant can be added by means of a syringe.
[0018] The diameter of the filling port in the above technical solution is 0.5-1mm.
[0019] The beneficial effect of the above technical solution is that it can meet the requirements of the needle insertion of the syringe.
[0020] In the above technical solution, an annular enclosure protrudes upward from the middle of the upper end of the partition, the position where the vertical rod passes through the partition is located in the annular enclosure, the annular enclosure is embedded with an annular expansion and contraction ring, and the vertical rod is sleeved in the expansion and contraction ring.
[0021] The beneficial effect of the above technical solution is that it can further improve the sealing performance at the position where the vertical rod passes through the partition.
[0022] In the above technical solution, the upper end of the annular enclosure is provided with an inner flange to press the expansion and contraction ring tightly inside the annular enclosure.
[0023] The beneficial effect of the above technical solution is that it makes it difficult for the expansion and contraction ring to loosen from the upper end of the annular enclosure. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] Figure 1 This is a schematic structural diagram of the separation of the bottle cap and the bottle body in Example 1 of the utility model;
[0025] Figure 2 This is a schematic diagram of the structure of the bottle cap and the bottle body when they are threadedly connected in Example 1 of the utility model;
[0026] Figure 3 This is a schematic structural diagram of the separation of the bottle cap and the bottle body in Example 2 of the utility model;
[0027] Figure 4 for Figure 3 A magnified schematic diagram of a part in the figure;
[0028] Figure 5 This is a schematic structural diagram of the bottle cap and the bottle body when they are threadedly connected in Example 2 of the utility model.
[0029] In the figure: 1. bottle body; 11. bottle mouth; 2. bottle cap; 21. partition; 22. lower tank chamber; 23. upper chamber; 24. filling port; 25. flexible plug; 26. annular enclosure; 261. inner flange; 27. expansion and contraction ring; 3. vertical pole; 31. handle; 4. flexible rod. DETAILED DESCRIPTION
[0030] The principles and features of the present invention are described below. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.
[0031] Example 1
[0032] like Figure 1 and Figure 2As shown, this embodiment provides a suspension cell culture bottle, which includes a bottle body 1 and a bottle cap 2, wherein a bottle mouth 11 with an external thread is arranged at the middle of the upper end of the bottle body 1, and the bottle cap 2 is a groove-shaped cover with a groove facing downward, and an internal thread matching the bottle mouth 11 is arranged on the inner side wall of the bottle cap 2, and a vertical rod 3 is arranged in the middle of the bottle cap 2 and is sealed and slidably connected with the bottle mouth 11, and a flexible rod 4 is arranged at the lower end of the vertical rod 3, and the bottle cap 2 is threadedly connected with the bottle mouth 11, and The lower end of the vertical rod 3 extends into the bottle body 1, and the bottle body 1 contains a cell culture fluid. The upper end of the vertical rod 3 is rotated so that the cell culture fluid in the bottle body 1 can be stirred by the flexible rod 4, so that the bottle cap can be threadedly connected to the bottle mouth of the bottle body. At this time, the lower end of the vertical rod extends into the bottle body, and the flexible rod is also stretched out in the bottle body. The vertical rod can be driven to rotate horizontally by artificial rotation, and the vertical rod can also slide up and down relative to the bottle cap, so that different depths in the bottle body can be stirred.
[0033] In the above technical solution, the flexible rod 4 is vertically connected to the vertical rod 3, which has a simple structure and good stirring effect.
[0034] In the above technical solution, 2-4 flexible rods 4 are provided, and the plurality of flexible rods 4 are vertically spaced and distributed, and two adjacent flexible rods 4 are cross-distributed, so that the plurality of flexible rods can cooperate to stir the cell culture medium, thereby making the cells therein more evenly dispersed.
[0035] In the above technical solution, a handle 31 is also provided at the upper end of the vertical pole 3, so that it is more convenient to manually rotate the vertical pole.
[0036] Among them, the lower end of the bottle body described in this embodiment is a circular groove structure, and the groove is closed in a trumpet shape to form the bottle mouth.
[0037] The middle part of the flexible rod is vertically connected to the vertical rod, and two adjacent flexible rods are perpendicular to each other and vertically spaced about 0.5 cm apart.
[0038] The cross-section of the vertical pole is circular, and it can not only slide up and down, but also rotate circumferentially. The handle 31 can be cylindrical and coaxially mounted on the upper end of the vertical pole, and the side wall of the handle is covered with grooves to form a friction surface (which is beneficial to increase friction resistance, so that the thumb and index finger can pinch the handle and rub it to rotate).
[0039] The vertical rod can be a hard plastic rod, and the flexible rod can be a flexible plastic rod, which can be squeezed to bend and deform. After the external force is removed, it will return to a straight state. Its length is greater than the inner diameter of the bottle mouth. When it is inserted into the bottle body, it will bend and deform at the bottle mouth.
[0040] Example 2
[0041] Same as Example 1, except that Figure 3-Figure 5 As shown, in the above technical solution, a partition 21 is horizontally arranged inside the bottle cap 2, and the partition 21 divides the bottle cap 2 into a lower chamber 22 and an upper chamber 23. The internal thread is arranged on the inner side wall of the lower chamber 22. The vertical rod 3 passes through the upper end of the bottle cap 2 and the partition 21. The upper chamber 23 is used to contain disinfectant, which is used to disinfect the surface of the vertical rod 3, so as to prevent bacteria from entering the bottle body through the position where the vertical rod passes through the bottle cap.
[0042] In the above technical solution, the upper end of the bottle cap 2 is also provided with a filling port 24 connected to the upper chamber 23, and the filling port 24 is used to inject disinfectant into the upper chamber 23, so that the disinfectant in the upper chamber can be added or replaced.
[0043] In the above technical solution, the filling port 24 is also embedded with a flexible plug 25 (which can be a silicone plug, rubber or latex plug), so that the upper chamber can be sealed, and the disinfectant can be added by a syringe. The disinfectant can be a disinfectant alcohol solution with a concentration of 75vol%.
[0044] The filling port 24 in the above technical solution has a diameter of 0.5-1 mm, which can meet the requirements of the needle of the syringe.
[0045] In the above technical solution, the middle part of the upper end of the partition 21 is upwardly protruded with an annular enclosure 26, and the position where the vertical rod 3 passes through the partition 21 is located in the annular enclosure 26. The annular enclosure 26 is embedded with an annular expansion and contraction ring 27 (which can be a silicone ring or a latex ring), and the vertical rod 3 is sleeved in the expansion and contraction ring 27, which can further improve the sealing performance of the position where the vertical rod passes through the partition. The liquid level of the disinfectant is located above the annular enclosure.
[0046] In the above technical solution, the upper end of the annular enclosure 26 is provided with an inner flange 261 to press the expansion and contraction ring 27 into the annular enclosure 26, so that the expansion and contraction ring is not easy to loosen from the upper end of the annular enclosure.
[0047] When the flexible rod is pressed down to enter the bottle body, it is squeezed into a U shape at the bottle mouth, and when it is pulled out of the bottle body, it is squeezed into an N shape at the bottle mouth.
[0048] The bottle body in this embodiment is made of a transparent material (may be a transparent plastic part), and all materials of the suspension cell culture bottle must meet the requirement of being able to be sterilized in a high-pressure sterilizer.
[0049] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A suspension cell culture flask, characterized in that: The invention comprises a bottle body (1) and a bottle cap (2), wherein a bottle mouth (11) with an external thread is arranged at the middle of the upper end of the bottle body (1), the bottle cap (2) is a groove-shaped cover with a groove facing downward, an internal thread matching the bottle mouth (11) is arranged on the inner side wall of the bottle cap (2), a vertical rod (3) penetrating vertically and sealingly slidably connected to the bottle mouth (11) is arranged in the middle of the bottle cap (2), and a flexible rod (4) is arranged at the lower end of the vertical rod (3). The bottle cap (2) is threadably connected to the bottle mouth (11), and the lower end of the vertical rod (3) extends into the bottle body (1). The bottle body (1) contains a cell culture fluid. The upper end of the vertical rod (3) is rotated so that the flexible rod (4) stirs the cell culture fluid in the bottle body (1). A partition (21) is horizontally arranged inside the bottle cap (2), and the partition (21) divides the bottle cap (2) into a lower chamber and a lower chamber. (22) and an upper chamber (23), the internal thread being arranged on the inner side wall of the lower chamber (22), the vertical rod (3) penetrating the upper end of the bottle cap (2) and the partition (21), the upper chamber (23) being used to contain disinfectant for disinfecting the surface of the vertical rod (3); the upper end of the bottle cap (2) is also provided with a filling port (24) communicating with the upper chamber (23), the filling port (24) Used to inject disinfectant into the upper chamber (23); a flexible plug (25) is also embedded at the filling port (24); an annular enclosure (26) is protruding upward at the middle of the upper end of the partition (21), and the position where the vertical rod (3) passes through the partition (21) is located in the annular enclosure (26), and an annular expansion and contraction ring (27) is embedded in the annular enclosure (26), and the vertical rod (3) is sleeved in the expansion and contraction ring (27).
2. The suspension cell culture bottle according to claim 1, characterized in that: The flexible rod (4) is vertically connected to the vertical rod (3).
3. The suspension cell culture bottle according to claim 2, characterized in that: The number of the flexible rods (4) is 2 to 4, and the plurality of the flexible rods (4) are vertically spaced and distributed, and two adjacent flexible rods (4) are cross-distributed.
4. The suspension cell culture bottle according to claim 1, characterized in that: A handle (31) is also provided at the upper end of the upright pole (3).
5. The suspension cell culture bottle according to claim 1, characterized in that: The filling port (24) has a diameter of 0.5-1 mm.
6. The suspension cell culture bottle according to claim 1, characterized in that: The upper end of the annular enclosure (26) is provided with an inner flange (261) to press the expansion and contraction ring (27) tightly inside the annular enclosure (26).