Tissue cell immunolabeling instrument capable of shortening dyeing time

Abstract

The utility model discloses a histocyte immunolabeling instrument for shortening staining time, which comprises a shell, a labeling turntable and a slide clamp, the middle part of the upper surface of the shell is connected with the labeling turntable through a rotating shaft, the inner wall of one side of the shell is connected with a driving motor, the output end of the driving motor is in key connection with a driving gear, and the driving gear is in key connection with the slide clamp. The driving gear and the driven gear are connected through a transmission toothed belt, a cleaning opening is formed in the side, away from the boss, of the cover plate, a cleaning water tank is arranged on the side, close to the cleaning opening, of the shell, one side of the cleaning water tank communicates with a water pipe, and a spray head is connected to the end, away from the cleaning water tank, of the water pipe. The spray head is used for spraying and cleaning the slide clamps at the cleaning opening, the driving motor is matched to drive the marking turntable to rotate so as to clean the slide clamps in sequence, centrifugal force generated by rotation of the marking turntable after cleaning is used for spin-drying, and therefore the cleanliness of the device is kept, and the situation that the follow-up marking effect is affected due to bacterium breeding is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of cell immune labeling instruments, in particular to a tissue cell immune labeling instrument capable of shortening staining time. Background Art

[0002] The biomedical industry often needs to perform operations such as immunolabeling on cells, which includes a series of steps such as using glass slides to carry samples, adding reagents, and spinning. Application number CN201611246054.1 has been retrieved, which is a fully automatic cell immunolabeling instrument. The fully automatic cell immunolabeling instrument provided by the invention can effectively improve the efficiency of cell labeling.

[0003] Similar to the above-mentioned fully automatic cell immunolabeling instrument: although the efficiency of cell labeling can be effectively improved by cooperating with the first liquid adding pump and the first liquid adding hole, the current cell immunolabeling instrument often does not have a cleaning function. During long-term use, bacteria and other impurities are easily bred at the slide clamp. It does not have a separate cleaning function, and the bacteria bred at the slide clamp can easily affect the subsequent labeling results.

[0004] Therefore, in view of this, the existing structure and defects are studied and improved, and a tissue cell immunolabeling instrument with shortened staining time is proposed to achieve a more practical purpose. Utility Model Content

[0005] The purpose of the utility model is to provide a tissue cell immunolabeling instrument that shortens the staining time, so as to solve the problem that the above-mentioned fully automatic cell immunolabeling instrument proposed in the above-mentioned background technology often does not have a cleaning function at this stage, and bacteria and other impurities are easily bred at the slide clamp during long-term use. It does not have a separate cleaning function, and the bacteria bred at the slide clamp are easy to affect the subsequent labeling results.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a tissue cell immunolabeling instrument for shortening staining time, comprising a shell, a marking turntable and a glass slide clamp, wherein the middle part of the upper surface of the shell is connected to the marking turntable via a rotating shaft, the glass slide clamp is arranged at the top edge of the marking turntable, the outer wall key of the rotating shaft is connected to a driven gear, the inner wall of one side of the shell is connected to a driving motor, the output end key of the driving motor is connected to a driving gear, and the driving gear and the driven gear are connected by a transmission toothed belt.

[0007] Furthermore, a cover plate is provided at the top of the marking turntable, a first liquid adding hole is arranged on one side edge of the cover plate, a first liquid adding pump is arranged on the upper side of the shell, the first liquid adding pump corresponds to the first liquid adding hole, and the first liquid adding hole and the glass slide clamp are on the same axis.

[0008] Furthermore, a boss is provided on one side of the first liquid adding hole, a second liquid adding hole is provided on one side of the boss, a test tube is arranged on one side of the second liquid adding hole, the second liquid adding hole and the test tube are in the same straight line, and a movable groove is provided on one side of the shell, and the movable groove is parallel to the boss.

[0009] Furthermore, the bottom of the movable groove is connected to a screw through a bearing, the outer wall of the screw is threadedly connected to a movable threaded block, a forward and reverse motor is provided on one side of the movable groove, the output end of the forward and reverse motor is connected to the screw, the top of the movable threaded block is connected to an electric telescopic rod, the top of the electric telescopic rod passes through the movable groove and is connected to a fixed box, a needle tube is provided at the bottom of one end of the fixed box, and a second liquid adding pump is provided inside the fixed box.

[0010] Furthermore, a cleaning port is provided on the side of the cover plate away from the boss, a cleaning water tank is provided on the side of the shell close to the cleaning port, one side of the cleaning water tank is connected to a water pipe, a water pump is provided in the middle of the water pipe, and a nozzle is connected to the end of the water pipe away from the cleaning water tank, and the spray corresponds to the cleaning port.

[0011] Furthermore, an ultraviolet sterilization lamp is arranged on one side of the top of the shell, a first liquid storage box is arranged in the bottom inner cavity of the shell, a second liquid storage box is arranged on one side of the first liquid storage box, and the first liquid storage box is connected to the first liquid adding hole through a conduit.

[0012] Compared with the prior art, the utility model has the following beneficial effects: a tissue cell immune labeling instrument that shortens the staining time, uses a nozzle to spray and clean the glass slide clamp at the cleaning port, cooperates with a driving motor to drive the marking turntable to rotate so as to clean the glass slide clamp in sequence, and after cleaning, the centrifugal force generated by the rotation of the marking turntable is used to dry, thereby maintaining the cleanliness of the device to avoid bacterial growth and thus affecting the subsequent marking effect. The specific contents are as follows:

[0013] A cleaning port is provided on the side of the cover plate away from the boss, and the cleaning port and the glass slide clamp are on the same axis. A cleaning water tank is provided on the side of the shell body close to the cleaning port, and the cleaning water tank is filled with cleaning liquid. One side of the cleaning water tank is connected to a water pipe, and a water pump is provided in the middle of the water pipe. The end of the water pipe away from the cleaning water tank is connected to a nozzle, and the spraying corresponds to the cleaning port. The water pump can draw the cleaning liquid in the cleaning water tank to the nozzle through the water pipe, and spray the cleaning liquid from the cleaning port to the glass slide clamp through the nozzle. The marking turntable is driven by a driving motor to rotate, so that the glass slide clamp is cleaned in turn. After cleaning, the centrifugal force generated by the rotation of the marking turntable is used to spin dry, thereby maintaining the cleanliness of the device to avoid bacterial growth and affecting the subsequent marking effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a front view structural schematic diagram of a tissue cell immunolabeling instrument for shortening staining time according to the utility model;

[0015] Figure 2 This is a schematic diagram of the screw structure of a tissue cell immunolabeling instrument for shortening the staining time of the utility model;

[0016] Figure 3 This is a schematic diagram of the active gear structure of a tissue cell immunolabeling instrument for shortening the staining time of the utility model;

[0017] Figure 4 The utility model is a schematic diagram of the structure of a marking turntable of a tissue cell immunolabeling instrument for shortening the staining time.

[0018] In the figure: 1. shell; 2. marking turntable; 3. rotating shaft; 4. slide clamp; 5. driven gear; 6. driving motor; 7. driving gear; 8. transmission belt; 9. cover plate; 10. first liquid adding hole; 11. first liquid adding pump; 12. boss; 13. second liquid adding port; 14. test tube; 15. movable groove; 16. screw; 17. movable thread block; 18. forward and reverse motor; 19. electric telescopic rod; 20. fixed box; 201. second liquid adding pump; 21. needle tube; 22. cleaning port; 23. cleaning water tank; 24. water pipe; 25. water pump; 26. nozzle; 28. ultraviolet germicidal lamp; 29. ​​first liquid storage box; 30. second liquid storage box. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0020] See also Figure 1 - Figure 4The utility model provides a technical solution: a tissue cell immunolabeling instrument for shortening the staining time, comprising a shell 1, a labeling turntable 2 and a slide clamp 4. The middle part of the upper surface of the shell 1 is connected with the labeling turntable 2 through a rotating shaft 3, and the labeling turntable 2 plays a bearing role. The slide clamp 4 is arranged at the top edge of the labeling turntable 2. The outer wall of the rotating shaft 3 is keyed to a driven gear 5. The inner wall of one side of the shell 1 is connected to a driving motor 6. The output end of the driving motor 6 is keyed to a driving gear 7. The driving gear 7 and the driven gear 5 are connected through a transmission toothed belt 8. The driving motor 6 can drive the driving gear 7 to rotate, and the driving gear 7 drives the transmission toothed belt 8 to rotate, thereby driving the driven gear 5 to rotate. The driven gear 5 drives the rotating shaft 3 to rotate synchronously with the labeling turntable 2, thereby realizing the rotation and injection of the glass slide at the slide clamp 4. At the same time, the labeling turntable 2 can complete the drying work. After the work is completed, the centrifugal force generated by the high-speed rotation of the labeling turntable 2 driven by the driving motor 6 can throw the sample out.

[0021] A cover plate 9 is provided at the top of the marking turntable 2, and the cover plate 9 can be closed to prevent external dust from entering. A first liquid adding hole 10 is arranged on one side edge of the cover plate 9, and a first liquid adding pump 11 is arranged on the upper side of the shell 1. The first liquid adding pump 11 corresponds to the first liquid adding hole 10, and the first liquid adding hole 10 and the glass slide clamp 4 are on the same axis. The first liquid adding pump 11 pumps the first liquid storage box 29 to add reagent to the glass slide clamp 4 under the first liquid adding hole 10.

[0022] A boss 12 is provided on one side of the first liquid adding hole 10, and the boss 12 plays a fixing role. A second liquid adding hole 13 is provided on one side of the boss 12, and the second liquid adding hole 13 and the glass slide clamp 4 are on the same axis. A secondary antibody marked with a fluorescent dye can be added through the second liquid adding hole 13 to react with the primary antibody to form a fluorescent-labeled complex. A test tube 14 is arranged on one side of the second liquid adding hole 13, and the test tube 14 can be used to store reagents. The second liquid adding hole 13 and the test tube 14 are on the same straight line. A movable groove 15 is provided on one side of the shell 1, and the movable groove 15 is parallel to the boss 12.

[0023] The bottom of the movable groove 15 is connected with a screw rod 16 through a bearing, and the outer wall of the screw rod 16 is threadedly connected with a movable thread block 17. A forward and reverse motor 18 is arranged on one side of the movable groove 15, and the output end of the forward and reverse motor 18 is connected to the screw rod 16. The top of the movable thread block 17 is connected with an electric telescopic rod 19, and the top of the electric telescopic rod 19 passes through the movable groove 15 and is connected with a fixed box 20. A needle tube 21 is arranged at the bottom of one end of the fixed box 20, and a second liquid adding pump 201 is arranged inside the fixed box 20. When liquid addition is required, the screw rod 16 is driven to rotate by the electric telescopic rod 19, and the rotation of the screw 16 drives the movable thread block 17 to move, thereby driving the electric telescopic rod 19 to translate, and the fixed box 20 at the top of the electric telescopic rod 19 is fixed. The needle tube 21 is aligned with the top of the test tube 14, and the needle tube 21 is driven downward by the contraction of the electric telescopic rod 19, and the needle tube 21 is driven by the second liquid adding pump 201 to absorb the reagent in the test tube 14. After absorption, it is lifted by the electric telescopic rod 19, and the forward and reverse motor 18 is started. The needle tube 21 passing through the electric telescopic rod 19 and the fixed box 20 is driven by the forward and reverse motor 18 to move and align with the second liquid adding hole 13, and the reagent is discharged through the needle tube 21 to the glass slide clamp 4 at the bottom of the second liquid adding hole 13 by the second liquid adding pump 201 to drip the reagent, and different reagents are added to the sample in the glass slide clamp 4 for staining and marking by the cooperation of the first liquid adding pump 11 and the second liquid adding pump 201, thereby avoiding artificial marking, improving staining efficiency, and shortening staining time.

[0024] A cleaning port 22 is provided on the side of the cover plate 9 away from the boss 12, and the cleaning port 22 and the glass slide clamp 4 are on the same axis. A cleaning water tank 23 is provided on the side of the shell body 1 close to the cleaning port 22, and the cleaning water tank 23 is filled with cleaning liquid. One side of the cleaning water tank 23 is connected with a water pipe 24, and a water pump 25 is provided in the middle of the water pipe 24. The end of the water pipe 24 away from the cleaning water tank 23 is connected with a nozzle 26, and the nozzle 26 corresponds to the cleaning port 22. The water pump 25 can draw the cleaning liquid in the cleaning water tank 23 to the nozzle 26 through the water pipe 24, and spray the cleaning port 22 to the glass slide clamp 4 through the nozzle 26. The marking turntable 2 is driven by the driving motor 6 to rotate, so as to clean the glass slide clamp 4 in turn. After cleaning, the centrifugal force generated by the rotation of the marking turntable 2 is used to spin dry, thereby maintaining the cleanliness of the device to avoid bacterial growth and affecting the subsequent marking effect.

[0025] An ultraviolet sterilization lamp 28 is arranged on one side of the top of the shell 1. The ultraviolet sterilization lamp 28 turned on for a certain period of time can have a sterilization effect, reducing the growth of bacteria in the device and thus preventing bacteria from affecting the marking results. The bottom inner cavity of the shell 1 is provided with a first liquid storage box 29, and a second liquid storage box 30 is arranged on one side of the first liquid storage box 29. The second liquid storage box 30 can pour the solution into the test tube 14 to avoid excessive contact with the air. The first liquid storage box 29 is connected to the first liquid adding hole 10 by a conduit, and different reagents can be stored in the first liquid storage box 29 and the second liquid storage box 30 respectively.

[0026] Working principle: When using a tissue cell immunolabeling instrument that shortens the staining time, place the sample to be labeled on the glass slide clamp 4, start the first first liquid adding pump 11, and drip the first reagent onto the first glass slide clamp 4 through the first first liquid adding hole 10. The driving motor 6 can drive the active gear 7 to rotate, and the rotation of the active gear 7 drives the transmission belt 8 to rotate, thereby driving the driven gear 5 to rotate. The rotation of the driven gear 5 drives the rotating shaft 3 and the marking turntable 2 to rotate synchronously, so that the first glass slide clamp 4 is aligned with the second liquid adding hole 13, and the needle tube 21 is driven downward by the contraction of the electric telescopic rod 19, and the needle tube 21 is driven by the second liquid adding pump 201 to absorb the reagent in the test tube 14. After absorption, it is lifted by the electric telescopic rod 19, and the forward and reverse motor 18 is started. The electric telescopic rod 19 and the needle tube 21 of the fixed box 20 are driven by the forward and reverse motor 18. Move and align the second liquid adding hole 13, and use the second liquid adding pump 201 to discharge the reagent through the needle tube 21 to the glass slide clamp 4 at the bottom of the second liquid adding hole 13 to drip the reagent, and use the first liquid adding pump 11 and the second liquid adding pump 201 to add different reagents to the sample in the glass slide clamp 4 for staining and marking, and reciprocate in sequence to mark the sample to be marked placed on the glass slide clamp 4. When the marking is completed and cleaning is required, the water pump 25 can pump the cleaning liquid in the cleaning water tank 23 to the nozzle 26 through the water pipe 24, and spray the cleaning port 22 to the glass slide clamp 4 through the nozzle 26. The marking turntable 2 is driven by the driving motor 6 to rotate, so as to clean the glass slide clamp 4 in turn. After cleaning, the centrifugal force generated by the rotation of the marking turntable 2 is used to spin dry, thereby maintaining the cleanliness of the device to avoid bacterial growth and affecting the subsequent marking effect.

Claims

1. A tissue cell immunolabeling instrument for shortening staining time, comprising a housing (1), a labeling turntable (2) and a slide holder (4), characterized in that: The middle part of the upper surface of the shell (1) is connected to a marking turntable (2) via a rotating shaft (3); the glass slide clamp (4) is arranged at the top edge of the marking turntable (2); the outer wall of the rotating shaft (3) is keyed to a driven gear (5); the inner wall of one side of the shell (1) is connected to a driving motor (6); the output end of the driving motor (6) is keyed to a driving gear (7); the driving gear (7) and the driven gear (5) are connected via a transmission toothed belt (8).

2. The tissue cell immune labeling instrument for shortening the staining time according to claim 1, characterized in that: A cover plate (9) is arranged at the top of the marking turntable (2), and a first liquid adding hole (10) is arranged on one side edge of the cover plate (9). A first liquid adding pump (11) is arranged on one side above the shell (1), and the first liquid adding pump (11) corresponds to the first liquid adding hole (10). The first liquid adding hole (10) and the glass slide clamp (4) are on the same axis.

3. The tissue cell immune labeling instrument for shortening the staining time according to claim 2, characterized in that: A boss (12) is provided on one side of the first liquid adding hole (10), a second liquid adding hole (13) is provided on one side of the boss (12), a test tube (14) is arranged on one side of the second liquid adding hole (13), the second liquid adding hole (13) and the test tube (14) are located on the same straight line, and a movable groove (15) is provided on one side of the shell (1), and the movable groove (15) is parallel to the boss (12).

4. The tissue cell immune labeling instrument for shortening the staining time according to claim 3, characterized in that: The bottom of the movable groove (15) is connected to a screw rod (16) via a bearing, the outer wall of the screw rod (16) is threadedly connected to a movable thread block (17), a forward and reverse motor (18) is arranged on one side of the movable groove (15), the output end of the forward and reverse motor (18) is connected to the screw rod (16), the top of the movable thread block (17) is connected to an electric telescopic rod (19), the top end of the electric telescopic rod (19) passes through the movable groove (15) and is connected to a fixed box (20), a needle tube (21) is arranged at the bottom of one end of the fixed box (20), and a second liquid adding pump (201) is arranged inside the fixed box (20).

5. The tissue cell immune labeling instrument for shortening the staining time according to claim 3, characterized in that: A cleaning port (22) is provided on the side of the cover plate (9) away from the boss (12); a cleaning water tank (23) is provided on the side of the housing (1) close to the cleaning port (22); one side of the cleaning water tank (23) is connected to a water pipe (24); a water pump (25) is provided in the middle of the water pipe (24); an end of the water pipe (24) away from the cleaning water tank (23) is connected to a nozzle (26); the nozzle (26) corresponds to the cleaning port (22).

6. The tissue cell immune labeling instrument for shortening staining time according to claim 1, characterized in that: An ultraviolet sterilization lamp (28) is arranged on one side of the top of the shell (1), and a first liquid storage box (29) is arranged in the bottom inner cavity of the shell (1), and a second liquid storage box (30) is arranged on one side of the first liquid storage box (29), and the first liquid storage box (29) is connected to the first liquid adding hole (10) through a conduit.

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