Full-automatic fraction collector

Abstract

The utility model provides a full-automatic fraction collector which comprises liquid storage test tubes, a plurality of liquid storage test tubes are placed on the upper end face of a supporting base, a fraction assembly is arranged in the supporting base, and a collecting assembly is arranged at the upper end of the supporting base. The electric heating plate can heat the bottom of the liquid storage test tube, and the silicon rubber electric heating sheet which is electrified to work can perform auxiliary heating on the lower half part of the liquid storage test tube, so that the distillation efficiency of protein liquid in the liquid storage test tube is improved; most of distilled water vapor can upwards enter the guiding and conveying bent pipe in a concentrated manner through the sealing cover, and at the moment, the plurality of cooling fins can cool the guiding and conveying bent pipe, so that the water vapor entering the guiding and conveying bent pipe is cooled into a liquid state and slides to the right side; and then the connecting pipe and the falling guide hose can guide the liquid water steam in the guiding and conveying bent pipe into the collecting box in a centralized mode to be collected.

Description

Technical Field

[0001] The utility model relates to the technical field of protein fraction collection, in particular to a full-automatic fraction collector. Background Art

[0002] In the field of biochemical technology, efficient and accurate separation of target proteins is the basis of many studies. Reliable sample preparation and fractionation process are the key to successful proteomics research. Currently, commonly used protein separation methods include affinity chromatography, chromatography, precipitation, distillation, etc. For the operation of using protein analyzer to detect protein, distillation operation is its "core".

[0003] However, most fraction collectors in the prior art have low heating distillation efficiency when collecting proteins, resulting in a less than ideal protein collection rate, and are unable to collect the distilled water vapor well, which easily causes a portion of the distilled water vapor to escape to the outside, and the fractionation effect needs to be improved. Utility Model Content

[0004] The purpose of the utility model is to provide a fully automatic fraction collector to solve the problems raised in the above background technology.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A fully automatic fraction collector comprises a bottom substrate, a support base and a liquid storage test tube, wherein the upper end surface of the bottom substrate is fixedly connected to the support base, a plurality of liquid storage test tubes are placed on the upper end surface of the support base, a fraction assembly is arranged inside the support base, and the fraction assembly is used to heat and distill the liquid inside the liquid storage test tube, a collection assembly is arranged at the upper end of the support base, and the collection assembly is used to centrally collect water vapor distilled from the fraction assembly, and a lifting assembly is arranged at the rear side of the upper end surface of the bottom substrate, and the lifting assembly is used to move the collection assembly up and down.

[0007] Preferably, the distillation assembly includes a bottom baffle, a vibration motor, an insulation layer and an electric heating plate. The bottom baffle is installed on the lower end surface of the support base, a vibration motor is installed at the middle position of the lower end surface of the bottom baffle, an insulation layer is provided on the upper end surface of the bottom baffle, an electric heating plate is installed inside the support base, and the upper end surface of the electric heating plate is in contact with the bottom of the liquid storage test tube.

[0008] Preferably, the collecting assembly comprises a sealing cover, an extension tray, a collecting box, a guide hose, a guide bend, a supporting mesh and a connecting pipe; a sealing cover is installed on the upper end surface of the supporting base, a guide bend is fixedly connected to the upper end of the sealing cover, an outlet of the guide bend is connected to a connecting pipe, a lower end of the connecting pipe is connected to the guide hose, an extension tray is provided on the lower side of the right end surface of the sealing cover, a supporting mesh is placed inside the extension tray, and a collecting box is placed on the upper end surface of the supporting mesh.

[0009] Preferably, the lifting assembly includes a motor, a support back plate, a vertical screw rod, a guide groove, a connecting plate and an internal threaded through hole. The support back plate is fixedly connected to the rear side of the upper end surface of the bottom substrate, a motor is installed on the upper end surface of the support back plate, a vertical screw rod is installed on the lower end of the motor, a guide groove is provided on the front end surface of the support back plate, a connecting plate is arranged inside the guide groove, and an internal threaded through hole is provided inside the connecting plate.

[0010] Preferably, support rods are symmetrically inserted on the left and right sides and the front and back sides of the upper end surface of the support base, a limiting plate is provided on the upper end of the support rod, a silicone rubber heating sheet is provided inside the limiting plate, and the inner diameter of the silicone rubber heating sheet is the same as the outer diameter of the liquid storage tube.

[0011] Preferably, the annular side surface of the guide bend is provided with multiple heat sinks, and the specifications of the multiple heat sinks are the same. The guide bend is higher on the left and lower on the right. A sealing strip is provided on the lower end surface of the sealing cover. The sealing cover is made of transparent acrylic. A fixed throat clamp is installed on the upper side of the annular side surface of the guide hose, and a docking tube is provided at the upper end of the collection box.

[0012] Preferably, a rubber vibration isolation pad is adhered to the lower end surface of the bottom substrate, a sealing groove is provided on the upper end surface of the support base, and mounting through holes are symmetrically provided on the left and right sides of the bottom substrate.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] 1. By energizing the electric heating plate and the silicone rubber electric heating sheet respectively, the electric heating plate can heat the bottom of the liquid storage test tube, and the energized silicone rubber electric heating sheet can assist in heating the lower half of the liquid storage test tube, thereby accelerating the distillation efficiency of the protein liquid inside the liquid storage test tube, thereby ensuring the collection rate of the protein;

[0015] 2. During the heating and distillation process of the fractionation component on the protein liquid in the liquid storage test tube, most of the distilled water vapor will pass through the sealing cover and enter the guide elbow. At this time, multiple heat sinks will dissipate heat and cool the guide elbow, so that the water vapor entering the guide elbow will be cooled into liquid and slide to the right. Then, the connecting pipe and the guide hose will guide the liquid water vapor in the guide elbow to the collection box for collection, so as to prevent the distilled water vapor from escaping to the outside easily.

[0016] 3. By controlling the motor to rotate in reverse or forward direction, the vertical screw rod can drive the connecting plate to move up and down through the internal threaded through hole, and the connecting plate will drive the sealing cover to move up and down, so that the user can automatically control the lifting and lowering of the sealing cover according to needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the main structure of the utility model;

[0018] Figure 2 This is a structural diagram of the utility model after the sealing cover is moved upward and opened;

[0019] Figure 3 It is a front cross-sectional view of the fractionation component and the collection component of the utility model;

[0020] Figure 4 This is a structural diagram of the sealing cover in the utility model;

[0021] Figure 5 It is a partial structural diagram of the collection component in the utility model.

[0022] In the figure: 1. bottom substrate; 11. rubber seismic isolation pad; 2. support base; 21. sealing groove; 3. fractionation assembly; 31. limit plate; 32. silicone rubber electric heating plate; 33. support rod; 34. bottom baffle; 35. vibration motor; 36. insulation layer; 37. electric heating plate; 4. collection assembly; 41. sealing cover; 411. sealing strip; 42. extension support plate; 43. collection box; 431. docking tube; 44. guide hose; 441. fixed throat clamp; 45. guide elbow; 46. heat sink; 47. support mesh; 48. connecting pipe; 5. lifting assembly; 51. motor; 52. support back plate; 53. vertical screw rod; 54. guide groove; 55. connecting plate; 56. internal threaded through hole; 6. liquid storage tube. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] See also Figure 1-5 , the utility model provides a technical solution:

[0025] Embodiment 1:

[0026] A fully automatic fraction collector comprises a bottom substrate 1, a support base 2 and a liquid storage tube 6. The upper end surface of the bottom substrate 1 is fixedly connected to the support base 2, and the support base 2 can support the liquid storage tube 6 and the fraction assembly 3. A plurality of liquid storage tubes 6 are placed on the upper end surface of the support base 2. The plurality of liquid storage tubes 6 are convenient for collecting and temporarily storing the protein liquid to be fractionated. A rubber isolation pad 11 is pasted on the lower end surface of the bottom substrate 1. The rubber isolation pad 11 prevents the bottom substrate 1 from generating resonance noise when the vibration motor 35 is working. The left and right sides of the bottom substrate 1 are A mounting through hole is provided, which facilitates users to install and fix the bottom substrate 1. A control panel is installed on the front side of the upper end of the bottom substrate 1, and the control panel is connected to the silicone rubber heating sheet 32, the electric heating plate 37, the vibration motor 35 and the motor 51 through wires. The control panel facilitates users to control the silicone rubber heating sheet 32, the electric heating plate 37, the vibration motor 35 and the motor 51 respectively. Since the working principle and internal detailed structure of the control panel are relatively mature technologies in the prior art, they will not be described in detail here.

[0027] A distillation component 3 is arranged inside the support base 2, and the distillation component 3 is used to heat and distill the liquid inside the liquid storage tube 6. A collection component 4 is arranged on the upper end of the support base 2, and the collection component 4 is used to centrally collect the water vapor distilled from the distillation component 3. A lifting component 5 is arranged on the rear side of the upper end surface of the bottom substrate 1, and the lifting component 5 is used to move the collection component 4 up and down.

[0028] The fractionation assembly 3 includes a bottom baffle 34, a vibration motor 35, an insulation layer 36 and an electric heating plate 37. The bottom baffle 34 is installed on the lower end surface of the support base 2, and the bottom baffle 34 can support the vibration motor 35 and the insulation layer 36. The vibration motor 35 is installed in the middle position of the lower end surface of the bottom baffle 34. When working, the vibration motor 35 can drive the multiple liquid storage tubes 6 to vibrate through the bottom baffle 34 and the support base 2, so that the protein liquid in the multiple liquid storage tubes 6 can precipitate and stratify faster. The upper end surface of the bottom baffle 34 is provided with an insulation layer 36, and the insulation layer 36 is made of insulation cotton. The insulation layer 36 made of insulation cotton makes the bottom baffle 34 better insulated and heat-preserving properties, thereby preventing the heat generated by the electric heating plate 37 from being quickly lost to the outside through the bottom baffle 34.

[0029] An electric heating plate 37 is installed inside the support base 2, and the upper end surface of the electric heating plate 37 is in contact with the bottom of the liquid storage test tube 6. When working, the electric heating plate 37 can heat the bottom of multiple liquid storage test tubes 6 to distill the protein liquid inside. Support rods 33 are symmetrically inserted on the left and right sides and the front and back sides of the upper end surface of the support base 2. The support rods 33 and the limiting plate 31 are an integrated structure. The support rods 33 can support the limiting plate 31. The upper end of the support rod 33 is provided with a limiting plate 31, and the limiting plate 31 can support the silicone rubber electric heating sheet 32. A silicone rubber electric heating sheet 32 ​​is arranged inside the limiting plate 31, and the inner diameter of the silicone rubber electric heating sheet 32 ​​is the same as the outer diameter of the liquid storage test tube 6. The number of silicone rubber electric heating sheets 32 is the same as the number of liquid storage test tubes 6. When powered on, multiple silicone rubber electric heating sheets 32 can perform auxiliary heating on the lower half of multiple liquid storage test tubes 6, thereby accelerating the distillation efficiency of the protein liquid inside the liquid storage test tube 6.

[0030] Embodiment 2:

[0031] On the basis of Example 1, most of the water vapor distilled in this example will pass upward through the sealing cover 41 and enter the guide bend 45. At this time, multiple heat sinks 46 will dissipate heat and cool the guide bend 45, so that the water vapor entering the guide bend 45 can be cooled into liquid and slide to the right. Then, the connecting pipe 48 and the guide hose 44 will guide the liquid water vapor in the guide bend 45 to the collection box 43 for collection, so as to prevent the distilled water vapor from easily escaping to the outside.

[0032] The collecting assembly 4 comprises a sealing cover 41, an extending support plate 42, a collecting box 43, a guide hose 44, a guide bend 45, a supporting mesh 47 and a connecting pipe 48. The sealing cover 41 is installed on the upper end surface of the supporting base 2. The sealing cover 41 is made of transparent acrylic. The sealing cover 41 made of transparent acrylic can not only concentrate the distilled gas into the guide bend 45, but also facilitate the user to observe the internal situation through the sealing cover 41. The upper end of the sealing cover 41 is fixedly connected with the guide bend 45. The guide bend 45, the sealing cover 41 and the connecting pipe 48 are all integrated structures. The guide bend 45 is high on the left and low on the right. The guide bend 45 with high on the left and low on the right is convenient for the liquid that enters the interior and is cooled to automatically flow to the right.

[0033] The outlet of the guide bend 45 is connected to a connecting pipe 48, which can guide the liquid in the guide bend 45 to the guide hose 44. The lower end of the connecting pipe 48 is connected to the guide hose 44. The guide hose 44 can not only guide the liquid guided down by the connecting pipe 48 to the docking pipe 431, but also has flexibility, which is convenient for users to separate or dock it with the docking pipe 431. A fixed throat hoop 441 is installed on the upper side of the annular side of the guide hose 44. The fixed throat hoop 441 can fix the upper end of the guide hose 44, and the collection box A docking tube 431 is provided at the upper end of 43, and the docking tube 431 and the collection box 43 are an integrated structure. The docking tube 431 facilitates the connection between the collection box 43 and the guide hose 44. An extension support plate 42 is provided on the lower side of the right end surface of the sealing cover 41, and the extension support plate 42 and the sealing cover 41 are an integrated structure. The extension support plate 42 can support the supporting mesh 47. A supporting mesh 47 is placed inside the extension support plate 42. The supporting mesh 47 can not only support the collection box 43, but also facilitate the contact between the external air and the bottom of the collection box 43 for ventilation and cooling.

[0034] A collecting box 43 is placed on the upper end surface of the supporting mesh 47, and the collecting box 43 can collect the distilled liquid. A plurality of heat sinks 46 are arranged on the annular side surface of the guiding bend 45, and the plurality of heat sinks 46 have the same specifications. The plurality of heat sinks 46 enable the guiding bend 45 to have better heat dissipation and cooling capabilities, so that the gas entering the inside of the guiding bend 45 is cooled to a liquid state. A sealing strip 411 is arranged on the lower end surface of the sealing cover 41, and the sealing strip 411 enables the sealing performance of the lower end surface of the sealing cover 41 to be better. A sealing groove 21 is provided on the upper end surface of the supporting base 2, and the sealing groove 21 matches the bottom of the sealing cover 41. The sealing groove 21 facilitates the bottom of the sealing cover 41 to be inserted into the upper end surface of the supporting base 2, thereby ensuring the sealing performance between the sealing cover 41 and the supporting base 2.

[0035] Embodiment three:

[0036] On the basis of the first embodiment, in this embodiment, by controlling the motor 51 to reverse or forward, the vertical screw rod 53 can drive the connecting plate 55 to move up and down through the internal threaded through hole 56, and the connecting plate 55 will drive the sealing cover 41 to move up and down, so that the user can automatically control the lifting and lowering of the sealing cover 41 according to needs.

[0037] The lifting assembly 5 includes a motor 51, a support back plate 52, a vertical screw rod 53, a guide groove 54, a connecting plate 55 and an internal threaded through hole 56. The support back plate 52 is fixedly connected to the rear side of the upper end surface of the bottom substrate 1. The support back plate 52 is connected to the bottom substrate 1 by welding. The support back plate 52 can support the vertical screw rod 53 and the motor 51. The motor 51 is installed on the upper end surface of the support back plate 52. The motor 51 can drive the vertical screw rod 53 to rotate. The vertical screw rod 53 is installed at the lower end of the motor 51. The connecting plate 55 has an internal threaded through hole. 56, and the internal threaded through hole 56 is meshed with the vertical screw rod 53, the meshed internal threaded through hole 56 and the vertical screw rod 53 can drive the connecting plate 55 to move up and down, the front end surface of the support back plate 52 is provided with a guide groove 54, the guide groove 54 is matched with the connecting plate 55, the guide groove 54 can limit and guide the connecting plate 55 to avoid shaking or deviation of the connecting plate 55 during lifting and using, a connecting plate 55 is arranged inside the guide groove 54, the connecting plate 55 and the sealing cover 41 are an integrated structure, and the connecting plate 55 can support the sealing cover 41.

[0038] Working principle: When multiple storage test tubes 6 storing protein liquid are placed on the upper end of the support base 2 (refer to Figure 2), the user controls the motor 51 to reverse through the control panel, so that the motor 51 can drive the vertical screw rod 53 to reverse together, and the reversed vertical screw rod 53 will drive the connecting plate 55 to move downward through the internal threaded through hole 56, and then the connecting plate 55 drives the sealing cover 41 to move downward into place, so that the sealing cover 41 can shield the multiple liquid storage test tubes 6; and then the electric heating plate 37 and the silicone rubber electric heating sheet 32 ​​are powered on respectively through the control panel. At this time, the powered electric heating plate 37 can heat the bottom of the multiple liquid storage test tubes 6, and the powered multiple silicone rubber electric heating sheets 32 can assist in heating the lower half of the multiple liquid storage test tubes 6, so as to speed up the distillation efficiency of the protein liquid inside the liquid storage test tubes 6; in this process, most of the distilled water vapor will be concentrated upward through the sealing cover 41 and enter the guide bend 45, and at this time, the multiple heat sinks 46 can The guide bend 45 is able to dissipate heat and cool down the guide bend 45, so that the water vapor entering the guide bend 45 is cooled down into liquid and slides to the right side, and then the connecting pipe 48 and the guide hose 44 will guide the liquid water vapor in the guide bend 45 to the collection box 43 for collection. At the same time, a part of the air will pass through the supporting mesh 47 and contact the bottom of the collection box 43 to assist in heat dissipation and cooling, so as to avoid excessive heat at the bottom of the collection box 43; when the liquid storage tube 6 is fractionated, the user first turns off the electric heating plate 37 and the silicone rubber electric heating plate 32 respectively, and then controls the motor 51 to rotate forward, so that the vertical screw rod 53 can drive the sealing cover 41 to move upward into place through the internal threaded through hole 56 and the connecting plate 55, and then the multiple liquid storage tubes 6 after fractionation can be taken away, and then the guide hose 44 is lifted up to separate it from the docking pipe 431, and the collection box 43 can be taken away at this time.

[0039] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A fully automatic fraction collector, comprising a bottom substrate (1), a support base (2) and a liquid storage tube (6), characterized in that: The upper end surface of the bottom substrate (1) is fixedly connected to a support base (2), a plurality of liquid storage test tubes (6) are placed on the upper end surface of the support base (2), a fractionation assembly (3) is arranged inside the support base (2), and the fractionation assembly (3) is used to heat and distill the liquid inside the liquid storage test tube (6), a collection assembly (4) is arranged at the upper end of the support base (2), and the collection assembly (4) is used to centrally collect water vapor distilled from the fractionation assembly (3), and a lifting assembly (5) is arranged on the rear side of the upper end surface of the bottom substrate (1), and the lifting assembly (5) is used to move the collection assembly (4) up and down.

2. A fully automatic fraction collector according to claim 1, characterized in that: The fractionation assembly (3) comprises a bottom baffle (34), a vibration motor (35), a heat insulation layer (36) and an electric heating plate (37); the bottom baffle (34) is installed on the lower end surface of the support base (2); the vibration motor (35) is installed at the middle position of the lower end surface of the bottom baffle (34); the heat insulation layer (36) is arranged on the upper end surface of the bottom baffle (34); the electric heating plate (37) is installed inside the support base (2), and the upper end surface of the electric heating plate (37) is in contact with the bottom of the liquid storage test tube (6).

3. A fully automatic fraction collector according to claim 1, characterized in that: The collecting assembly (4) comprises a sealing cover (41), an extension support plate (42), a collecting box (43), a guide hose (44), a guide bend pipe (45), a supporting mesh (47) and a connecting pipe (48); the sealing cover (41) is installed on the upper end surface of the supporting base (2); the upper end of the sealing cover (41) is fixedly connected to the guide bend pipe (45); the outlet of the guide bend pipe (45) is connected to the connecting pipe (48); the lower end of the connecting pipe (48) is connected to the guide hose (44); an extension support plate (42) is arranged on the lower side of the right end surface of the sealing cover (41); a supporting mesh (47) is placed inside the extension support plate (42); and the collecting box (43) is placed on the upper end surface of the supporting mesh (47).

4. A fully automatic fraction collector according to claim 1, characterized in that: The lifting assembly (5) comprises a motor (51), a support back plate (52), a vertical screw rod (53), a guide groove (54), a connecting plate (55) and an internal threaded through hole (56); the rear side of the upper end surface of the bottom substrate (1) is fixedly connected to the support back plate (52); the upper end surface of the support back plate (52) is equipped with a motor (51); the lower end of the motor (51) is equipped with a vertical screw rod (53); the front end surface of the support back plate (52) is provided with a guide groove (54); a connecting plate (55) is arranged inside the guide groove (54); and the connecting plate (55) is provided with an internal threaded through hole (56).

5. A fully automatic fraction collector according to claim 1, characterized in that: Support rods (33) are symmetrically inserted on the left and right sides and the front and rear sides of the upper end surface of the support base (2), and a limit plate (31) is arranged on the upper end of the support rod (33). A silicone rubber heating plate (32) is arranged inside the limit plate (31), and the inner diameter of the silicone rubber heating plate (32) is the same as the outer diameter of the liquid storage test tube (6).

6. A fully automatic fraction collector according to claim 3, characterized in that: The annular side surface of the guide bend pipe (45) is provided with a plurality of heat sinks (46), and the specifications of the plurality of heat sinks (46) are the same. The guide bend pipe (45) is higher on the left and lower on the right. The lower end surface of the sealing cover (41) is provided with a sealing strip (411). The sealing cover (41) is made of transparent acrylic. A fixed throat clamp (441) is installed on the upper side of the annular side surface of the guide hose (44). A butt joint pipe (431) is provided at the upper end of the collection box (43).

7. A fully automatic fraction collector according to claim 1, characterized in that: A rubber vibration isolation pad (11) is adhered to the lower end surface of the bottom substrate (1), a sealing groove (21) is provided on the upper end surface of the support base (2), and mounting through holes are symmetrically provided on the left and right sides of the inside of the bottom substrate (1).

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