Liver perfusion system and detection device

Abstract

The utility model discloses a liver perfusion system which comprises an organ basin, a first circulation pipeline, a second circulation pipeline and a third circulation pipeline, the first circulation pipeline comprises a first output pipe and a first perfusion pipe, the first output pipe is used for perfusate to flow in from the organ basin, and the first perfusion pipe is used for perfusate to flow out from the first output pipe; the second circulating pipeline comprises a second output pipe and a second infusion pipe, the second output pipe is used for allowing the infusion liquid to flow in from the organ basin, and the second infusion pipe is used for allowing the infusion liquid to flow out from the second output pipe; the first pipe joint comprises a first opening, a second opening and a third opening, the first opening is communicated with the first output pipe, the second opening is communicated with the first infusion pipe, and the third opening is used for being communicated with a detection device; the second pipe joint comprises a fourth opening, a fifth opening and a sixth opening, the fourth opening is communicated with the second output pipe, the fifth opening is communicated with the second infusion pipe, and the sixth opening is used for being communicated with a detection device. According to the liver perfusion system disclosed by the utility model, a worker can conveniently carry out sterile detection.

Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, in particular to a liver perfusion system and a detection device. Background Art

[0002] In the related art, during liver transplantation, the liver needs to be placed in a liver perfusion system for preservation before being transferred to the human body. The liver perfusion system can provide blood and nutrients to the liver during perfusion, thereby keeping the liver active.

[0003] Furthermore, after the production of the liver perfusion system is completed, it needs to be sterilized. After the sterilization is completed, the staff will perform a sterility test on the liver perfusion system to determine whether the liver perfusion system is sterile. Existing liver perfusion systems usually use a membrane filtration method for sterility testing. However, when using the membrane filtration method to perform a sterility test on the liver perfusion system, the liver perfusion system is not convenient for the staff to operate it, resulting in a low efficiency of the sterility test. Utility Model Content

[0004] The utility model aims to solve at least one of the technical problems existing in the prior art. To this end, the utility model provides a liver perfusion system, which can facilitate workers to perform sterility testing.

[0005] The utility model also provides a detection device.

[0006] The liver perfusion system according to the first embodiment of the utility model comprises:

[0007] An organ basin having a storage cavity for accommodating an organ;

[0008] a first circulation pipeline, comprising a first output tube and a first perfusion tube, wherein one end of the first output tube is in communication with the organ basin, and the other end of the first output tube is in communication with the first perfusion tube, the first output tube is located outside the organ basin, the first perfusion tube is located in the organ basin, the first output tube is used for perfusion fluid to flow into the organ basin, and the first perfusion tube is used for perfusion fluid to flow out of the first output tube;

[0009] a second circulation pipeline, comprising a second output tube and a second perfusion tube, wherein one end of the second output tube is in communication with the organ basin, and the other end of the second output tube is in communication with the second perfusion tube, the second output tube is located outside the organ basin, the second perfusion tube is located in the organ basin, the second output tube is used for the perfusion liquid to flow into the organ basin, and the second perfusion tube is used for the perfusion liquid to flow out of the second output tube;

[0010] A first pipe joint, comprising a first opening, a second opening and a third opening which are connected to each other, wherein the first opening is connected to the first output pipe, the second opening is connected to the first perfusion pipe, and the third opening is used to connect to the detection device;

[0011] The second pipe joint includes a fourth opening, a fifth opening and a sixth opening which are connected to each other, wherein the fourth opening is connected to the second output pipe, the fifth opening is connected to the second perfusion pipe, and the sixth opening is used to connect to the detection device.

[0012] The liver perfusion system according to the embodiment of the utility model has at least the following beneficial effects: the detection device is connected to the third opening and the sixth opening respectively, so that the buffer can be placed in the storage cavity of the organ basin, and then the buffer can be circulated. At this time, the buffer can pass through the storage cavity, the first output tube, the first perfusion tube, the second output tube, the second perfusion tube, etc. After the buffer is circulated multiple times, the buffer can be tested for sterility. If the buffer test result is bacterial, then it can be determined that the liver perfusion system is bacterial and its product is unqualified. On the contrary, if the buffer test result is sterile, then it can be determined that the liver perfusion system is sterile and its product is qualified. Specifically, since the third opening and the sixth opening of the liver perfusion system in the present application can be connected to the detection device, when the liver perfusion system is sterile, it only needs to connect the third opening and the sixth opening to pass the detection device. In this way, the liver perfusion system can facilitate the staff to perform sterility testing.

[0013] According to some embodiments of the present invention, the liver perfusion system further includes a first oxygenator and a second oxygenator, wherein the first oxygenator is in communication with the first circulation line, and the second oxygenator is in communication with the second circulation line.

[0014] According to some embodiments of the liver perfusion system of the present invention, the liver perfusion system further includes a first thrombus filter and a second thrombus filter, wherein the first thrombus filter is in communication with the first circulation pipeline, and the second thrombus filter is in communication with the second circulation pipeline.

[0015] According to some embodiments of the liver perfusion system of the present invention, the liver perfusion system also includes a first driving member and a second driving member, the first driving member is connected to the first circulation pipeline, the second driving member is connected to the second circulation pipeline, the first driving member is used to drive the perfusion fluid to flow in the first circulation pipeline, and the second driving member is used to drive the perfusion fluid to flow in the second circulation pipeline.

[0016] According to some embodiments of the liver perfusion system of the present invention, the liver perfusion system further includes a first blocking member, and the first blocking member is used to block the third opening.

[0017] According to some embodiments of the liver perfusion system of the present invention, the liver perfusion system further includes a second blocking member, and the second blocking member is used to block the sixth opening.

[0018] According to some embodiments of the liver perfusion system of the present invention, the liver perfusion system further comprises an upper cover, which is detachably connected to the organ basin and is used to seal the opening of the storage cavity.

[0019] According to some embodiments of the liver perfusion system of the present invention, the organ basin includes a main body and a protruding portion, the main body is provided with the storage cavity, the protruding portion is connected to the main body and protrudes toward the upper cover, the upper cover is provided with a positioning hole, and the protruding portion is arranged in the positioning hole.

[0020] According to a detection device of a second aspect of the present invention, the detection device is used to detect the liver perfusion system described in any one of the first aspect embodiments, and the detection device includes:

[0021] A pipeline tool, comprising an input pipe and an output pipe connected to each other, wherein the input pipe is connected to the third opening and the sixth opening respectively, and one end of the output pipe is connected to the organ basin;

[0022] A peristaltic pump is connected to the output pipe, and the peristaltic pump is used to drive the buffer solution in the pipeline tooling to flow.

[0023] The detection device according to the embodiment of the utility model has at least the following beneficial effects: after the third opening and the sixth opening are connected by the pipeline tooling, and one end of the output tube is connected to the organ basin, the buffer solution can be circulated, and then the buffer solution is circulated in the liver perfusion system by the peristaltic pump, and finally the buffer solution after the flow is subjected to a sterility test. If the buffer solution test result is that there are bacteria, then it can be determined that the liver perfusion system is bacteria and its product is unqualified. On the contrary, if the buffer solution test result is sterile, then it can be determined that the liver perfusion system is sterile and its product is qualified. In this way, the detection device can detect whether the liver perfusion system is sterile.

[0024] According to the detection device of some embodiments of the present utility model, the input pipe includes a first section and a second section, the first section is connected to the third opening, the second section is connected to the sixth opening, the pipeline tooling also includes a third joint, the third joint includes a seventh opening, an eighth opening and a ninth opening that are connected to each other, the seventh opening is connected to the first section, the eighth opening is connected to the second section, and the ninth opening is connected to the output pipe.

[0025] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The present invention is further described below with reference to the accompanying drawings and embodiments, wherein:

[0027] Figure 1 A schematic diagram of a liver perfusion system according to some embodiments of the present invention;

[0028] Figure 2 for Figure 1 The enlarged schematic diagram of point A in the middle;

[0029] Figure 3 It is a schematic diagram of the process of the sterility detection method of some embodiments of the utility model;

[0030] Figure 4 A schematic diagram of a liver perfusion system and a detection device according to a first embodiment of the utility model;

[0031] Figure 5 Schematic diagram of a detection device in some embodiments of the utility model;

[0032] Figure 6 This is a schematic diagram of a liver perfusion system and a detection device according to a second embodiment of the present invention.

[0033] Reference numerals:

[0034] Liver perfusion system 10, organ basin 100, main body 110, protrusion 120, first circulation pipeline 200, first output pipe 210, first perfusion pipe 220, second circulation pipeline 300, second output pipe 310, second perfusion pipe 320, first pipe joint 400, first opening 410, second opening 420, third opening 430, second pipe joint 500, fourth opening 510, fifth opening 520, sixth opening 530, first oxygenator 600, second oxygenator 700, first thrombus filter 800, second thrombus filter 900, upper cover 1000;

[0035] Detection device 20 , pipeline tooling 30 , input pipe 31 , first section 32 , second section 33 , output pipe 34 , third joint 35 , seventh opening 36 , eighth opening 37 , ninth opening 38 , and peristaltic pump 40 . DETAILED DESCRIPTION

[0036] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0037] In the description of the present invention, it should be understood that descriptions involving orientation, such as up, down, front, back, left, right, etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0038] In the description of the present utility model, "several" means more than one, "many" means more than two, "greater than", "less than", "exceed" etc. are understood to exclude the number itself, and "above", "below", "within" etc. are understood to include the number itself. If there is a description of "first" or "second", it is only used to distinguish the technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0039] In the description of the present invention, unless otherwise clearly defined, terms such as setting, installing, connecting, etc. should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in the present invention based on the specific content of the technical solution.

[0040] In the description of the utility model, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0041] Please refer to Figure 1 , Figure 2 and Figure 4In some embodiments, the liver perfusion system 10 includes: an organ basin 100, a first circulation pipeline 200, a second circulation pipeline 300, a first pipe joint 400 and a second pipe joint 500. The organ basin 100 has a storage cavity, and the storage cavity is used to accommodate organs. For example, the storage cavity can accommodate a liver, so that the transplanted liver can be placed in the storage cavity to maintain its activity. After the liver is placed in the organ basin 100, it needs to be connected to the hepatic artery and portal vein of the liver through the first circulation pipeline 200 and the second circulation pipeline 300 respectively, so as to supply blood and nutrients to the liver. Among them, in this application, blood and nutrient solution are all referred to as perfusion fluid.

[0042] The first circulation pipeline 200 includes a first output tube 210 and a first perfusion tube 220. One end of the first output tube 210 is connected to the organ basin 100, and the other end of the first output tube 210 is connected to the first perfusion tube 220. The first output tube 210 is located outside the organ basin 100, and the first perfusion tube 220 is located in the organ basin 100. After the first perfusion tube 220 is fixed in the storage cavity, the first perfusion tube 220 can be conveniently connected to the liver, for example, the first perfusion tube 220 is connected to the hepatic artery of the liver. The first output tube 210 is used to supply perfusion fluid to flow into the organ basin 100, and the first perfusion tube 220 is used to supply perfusion fluid to flow out of the first output tube 210. In this way, after the liver is placed in the storage cavity, the first circulation pipeline 200 can circulate the perfusion fluid in the liver to keep the liver active.

[0043] The second circulation pipeline 300 includes a second output tube 310 and a second perfusion tube 320. One end of the second output tube 310 is connected to the organ basin 100, and the other end of the second output tube 310 is connected to the second perfusion tube 320. The second output tube 310 is located outside the organ basin 100, and the second perfusion tube 320 is located in the organ basin 100. After the second perfusion tube 320 is fixed in the storage cavity, the second perfusion tube 320 can be conveniently connected to the liver, for example, the second perfusion tube 320 is connected to the portal vein of the liver. The second output tube 310 is used to supply perfusion fluid to flow into the organ basin 100, and the second perfusion tube 320 is used to supply perfusion fluid to flow out of the second output tube 310. In this way, after the liver is placed in the storage cavity, the second circulation pipeline 300 can circulate the perfusion fluid in the liver to keep the liver active.

[0044] The first pipe joint 400 includes a first opening 410, a second opening 420 and a third opening 430 that are interconnected. The first opening 410 is connected to the first output pipe 210, the second opening 420 is connected to the first perfusion pipe 220, and the third opening 430 is used to communicate with the detection device 20. The second pipe joint 500 includes a fourth opening 510, a fifth opening 520 and a sixth opening 530 that are interconnected. The fourth opening 510 is connected to the second output pipe 310, the fifth opening 520 is connected to the second perfusion pipe 320, and the sixth opening 530 is used to communicate with the detection device 20. Specifically, the detection device 20 is connected to the third opening 430 and the sixth opening 530 respectively, so that the buffer can be placed in the storage cavity of the organ basin 100, and then the buffer can be circulated. At this time, the buffer can pass through the storage cavity, the first output tube 210, the first perfusion tube 220, the second output tube 310 and the second perfusion tube 320, etc. After the buffer is circulated multiple times, the buffer can be tested for sterility. If the buffer test result is bacterial, then it can be determined that the liver perfusion system 10 is bacterial and its product is unqualified. On the contrary, if the buffer test result is sterile, then it can be determined that the liver perfusion system 10 is sterile and its product is qualified. Specifically, since the third opening 430 and the sixth opening 530 of the liver perfusion system 10 in the present application can be connected to the detection device 20, when the liver perfusion system 10 is sterile, it only needs to connect the third opening 430 and the sixth opening 530 to pass the detection by the detection device 20. In this way, the liver perfusion system 10 can facilitate the staff to perform sterility testing.

[0045] It should be added that after the processing of the liver perfusion system 10 is completed, a sterility test needs to be completed. Since the liver perfusion system 10 is provided with a circulation pipeline to allow the perfusion fluid to circulate in the liver, the liver perfusion system 10 is large in size and has many devices, and it is relatively difficult to perform sterility tests on multiple devices. However, the present application connects the detection device 20 through the first pipe joint 400 and the second pipe joint 500 respectively, so that the buffer solution can be circulated in the liver perfusion system 10. It can be imagined that when the buffer solution circulates in the liver perfusion system 10, if there are bacteria in the liver perfusion system 10, then the bacteria will contact the buffer solution. By testing the buffer solution, it can be known whether the liver perfusion system 10 is sterile. Among them, the buffer solution can be a sodium chloride-peptone buffer solution with a pH of 7.0.

[0046] It is further necessary to add that it is conceivable that, since the first perfusion tube 220 and the second perfusion tube 320 are to be connected to the liver, the diameters of the first perfusion tube 220 and the second perfusion tube 320 are smaller than the diameters of the first output tube 210 and the second output tube 310. If the liver perfusion system 10 is not provided with the first pipe joint 400 and the second pipe joint 500, and is directly connected to the detection device 20 through the first perfusion tube 220 and the second perfusion tube 320, then this requires that the diameters of the first perfusion tube 220 and the second perfusion tube 320 match the diameter of the detection device 20, but the diameter of the detection device 20 is generally larger than the diameter of the first perfusion tube 220, because this can facilitate the flow of the buffer solution, and increase the flow rate of the buffer solution, thereby increasing the detection speed. Therefore, after the first pipe joint 400 and the second pipe joint 500 are provided, the liver perfusion system 10 can be conveniently matched with the liver and the detection device 20.

[0047] For further information, please refer to Figure 1 In some embodiments, the liver perfusion system 10 further includes a first oxygenator 600 and a second oxygenator 700, wherein the first oxygenator 600 is in communication with the first circulation pipeline 200, and the second oxygenator 700 is in communication with the second circulation pipeline 300. Specifically, the first oxygenator 600 is in communication with the first circulation pipeline 200 in such a manner that the first oxygenator 600 is in communication with the first output pipe 210, and the first oxygenator 600 can discharge carbon dioxide in the perfusate and oxygenate blood and oxygen, so that the first circulation pipeline 200 can provide oxygen to the liver. The second oxygenator 700 is in communication with the second circulation pipeline 300 in such a manner that the second oxygenator 700 is in communication with the second output pipe 310, and the second oxygenator 700 can discharge carbon dioxide in the perfusate and oxygenate blood and oxygen, so that the second circulation pipeline 300 can provide oxygen to the liver.

[0048] It should be noted that, during the sterility test, the buffer solution may also pass through the first oxygenator 600 and the second oxygenator 700 to detect whether the first oxygenator 600 and the second oxygenator 700 are sterile.

[0049] For further information, please refer to Figure 1In some embodiments, the liver perfusion system 10 further includes a first embolic filter 800 and a second embolic filter 900, wherein the first embolic filter 800 is in communication with the first circulation pipeline 200, and the second embolic filter 900 is in communication with the second circulation pipeline 300. Specifically, the first embolic filter 800 is in communication with the first circulation pipeline 200 in the manner that the first embolic filter 800 is in communication with the first output tube 210. The second embolic filter 900 is in communication with the second circulation pipeline 300 in the manner that the second embolic filter 900 is in communication with the second output tube 310. The first embolic filter 800 and the second embolic filter 900 can be used to filter out various microemboli generated during the extracorporeal circulation of the liver, which can prevent embolism of the liver microvessels caused by various microemboli such as thrombi or gas emboli, and effectively improve the blood perfusion of the human microvessels.

[0050] It should be noted that, during the sterility test, the buffer solution may also pass through the first filter 800 and the second filter 900 to detect whether the first filter 800 and the second filter 900 are sterile.

[0051] For further information, please refer to Figure 1 In some embodiments, the liver perfusion system 10 further includes a first driving member and a second driving member, the first driving member is in communication with the first circulation pipeline 200, the second driving member is in communication with the second circulation pipeline 300, the first driving member is used to drive the perfusion liquid to flow in the first circulation pipeline 200, and the second driving member is used to drive the perfusion liquid to flow in the second circulation pipeline 300. The first driving member and the second driving member can both be centrifugal pumps, which can make the perfusion liquid flow, thereby realizing the continuous circulation of the perfusion liquid and maintaining liver activity.

[0052] Further, in some embodiments, the liver perfusion system 10 further includes a first blocking member, which is used to block the third opening 430, and the liver perfusion system 10 further includes a second blocking member, which is used to block the sixth opening 530. Specifically, after the liver perfusion system 10 is sterile tested, if the liver perfusion system 10 meets the requirements, in order to make the perfusion liquid flow out from the first output tube 210 instead of the third opening 430 in the subsequent use of the liver perfusion system 10, the third opening 430 can be blocked by the first blocking member, thereby ensuring that the perfusion liquid flows out from the first perfusion tube 220 instead of the third opening 430. Similarly, in order to make the perfusion liquid flow out from the second output tube 310 instead of the sixth opening 530 in the subsequent use of the liver perfusion system 10, the sixth opening 530 can be blocked by the second blocking member, thereby ensuring that the perfusion liquid flows out from the second perfusion tube 320 instead of the sixth opening 530.

[0053] For further information, please refer to Figure 1 and Figure 6In some embodiments, the liver perfusion system 10 further includes an upper cover 1000, which is detachably connected to the organ basin 100 and is used to seal the opening of the storage cavity. After the upper cover 1000 is connected to the organ basin 100, the upper cover 1000 can play a role in dust prevention.

[0054] Furthermore, after the upper cover 1000 is covered on the organ basin 100, the upper cover 1000 may move, so that the upper cover 1000 falls off the organ basin 100. Therefore, in order to solve this problem, a protrusion 120 can be provided on the organ basin 100. For details, please refer to Figure 1 and Figure 2 In some embodiments, the organ basin 100 includes a main body 110 and a protrusion 120, the main body 110 is provided with a storage cavity, the protrusion 120 is connected to the main body 110 and protrudes toward the upper cover 1000, the upper cover 1000 is provided with a positioning hole, and the protrusion 120 is arranged in the positioning hole. In this way, when the staff covers the upper cover 1000 on the organ basin 100, if the protrusion 120 does not touch the positioning hole, the upper cover 1000 cannot be covered, and the staff can know that the direction of the upper cover 1000 is wrong, and the protrusion 120 can be located in the positioning hole by adjusting the direction of the upper cover 1000. In addition, after the upper cover 1000 is covered on the organ basin 100, since the protrusion 120 is located in the positioning hole, the upper cover 1000 will not move. Specifically, when someone touches the organ basin 100, the upper cover 1000 will not slide off due to the impact of external force.

[0055] The following describes the sterility test method. Figure 3 and Figure 4 In some embodiments, a sterility detection method is used to perform a sterility detection on the liver perfusion system 10 of any one of the above embodiments, and the sterility detection method comprises the following steps:

[0056] S100, connecting the detection device 20 to the third opening 430 and the sixth opening 530 respectively:

[0057] S200, pouring a buffer into the organ basin 100, and then circulating the buffer;

[0058] S300, checking whether the buffer solution is sterile after multiple circulations.

[0059] Specifically, after the third opening 430 and the sixth opening 530 are connected through the detection device 20, and the buffer solution circulates in the liver perfusion system 10, the flowing buffer solution is finally subjected to a sterility test. If the buffer solution test result is bacterial, then it can be determined that the liver perfusion system 10 is bacterial and its product is unqualified. Conversely, if the buffer solution test result is sterile, then it can be determined that the liver perfusion system 10 is sterile and its product is qualified. In this way, the sterility detection method can detect whether the liver perfusion system 10 is sterile.

[0060] The specific process of the sterility detection method is introduced again below. 1. Use sterile tweezers to connect the first section 32 and the third opening 430, and connect the second section 33 and the sixth opening 530; 2. Connect the output tube 34 and the peristaltic pump 40, and then place one end of the output tube 34 in the storage chamber of the organ basin 100; 3. Pour about 1000ml of pH7.0 sodium chloride-peptone buffer into the organ basin 100 and cover the upper cover 1000; 4. Start to drive the buffer to circulate through the peristaltic pump 40, where the peristaltic condition is 500ml / min, and the peristaltic condition is 2min; 5. Transfer the buffer after the cycle to the bacteria collector 6. First turn on the bacteria collector (about 90rpm), and then turn on the peristaltic pump 40 (180ml / min) to filter the buffer into 3 independent filter cups respectively. After the flushing liquid is completely filtered, plug the bottom of the filter cup with a matching plug. (The needle and the pipe port are both burned with an alcohol lamp); 7. Add culture medium: 7.1. Insert the needle end of the disposable bacteria collector into a saline bottle containing thioglycollate fluid culture medium (FT) or tryptic soy broth (TSB), open the bacteria collector for suction filtration, and draw the culture medium into the filter cup. There should be at least one filter cup for each culture medium. The extra culture medium containing buffer is used as a positive control. Clamp the pipe with a pipe clamp, then cut the pipe of the filter cup and insert it into the air filter hole, and seal the filter cup. (The outer surface of the needle of the bacteria collector is quickly burned with an alcohol lamp); 7.2 Positive control: Inoculate a suspension of Staphylococcus aureus of no more than 100 cfu into a thioglycollate fluid medium (FT) containing one sample; 7.3 Negative control: Take 100 ml of thioglycollate fluid medium (FT) and 100 ml of tryptic soy bean liquid medium (TSB) as negative controls; 8. Cultivation and observation: Cultivate the disposable sterile filter cup containing the thioglycollate fluid medium (FT) at 30-35°C for 14 days, and the disposable bacteria collector containing tryptic soy bean liquid medium (TSB) at 20-25°C for 14 days. During the cultivation period, regular observation and record should be made to see if there is bacterial growth. If there is bacterial growth, then it is determined that there are bacteria in the liver perfusion system 10 and the liver perfusion system 10 is unqualified. If there is no bacterial growth, then it is determined that there is no bacteria in the liver perfusion system 10 and the liver perfusion system 10 is qualified.

[0061] The following describes the detection device 20, please refer to Figure 4 and Figure 5 In some embodiments, the detection device 20 is used to detect the liver perfusion system 10, and the detection device 20 includes: a pipeline fixture 30 and a peristaltic pump 40. The pipeline fixture 30 includes an input tube 31 and an output tube 34 that are connected to each other, the input tube 31 is connected to the third opening 430 and the sixth opening 530 respectively, and one end of the output tube 34 is connected to the organ basin 100. The peristaltic pump 40 is connected to the output tube 34, and the peristaltic pump 40 is used to drive the buffer solution in the pipeline fixture 30 to flow. Specifically, after the third opening 430 and the sixth opening 530 are connected through the pipeline fixture 30, and one end of the output tube 34 is connected to the organ basin 100, the buffer solution can circulate, and then the buffer solution is circulated in the liver perfusion system 10 through the peristaltic pump 40. Finally, the buffer solution after flowing is subjected to a sterility test. If the buffer solution test result is that there are bacteria, then it can be determined that the liver perfusion system 10 is bacteria and its product is unqualified. On the contrary, if the buffer solution test result is sterile, then it can be determined that the liver perfusion system 10 is sterile and its product is qualified. In this way, the detection device 20 can detect whether the liver perfusion system 10 is sterile.

[0062] For further information, please refer to Figure 5 In some embodiments, the input tube 31 includes a first section 32 and a second section 33, the first section 32 is connected to the third opening 430, the second section 33 is connected to the sixth opening 530, and the pipeline fixture 30 also includes a third joint 35, the third joint 35 includes a seventh opening 36, an eighth opening 37 and a ninth opening 38 that are connected to each other, the seventh opening 36 is connected to the first section 32, the eighth opening 37 is connected to the second section 33, and the ninth opening 38 is connected to the output tube 34. In this way, after the first section 32 and the second section 33 are connected to the output tube 34 through the third joint 35, the detection device 20 can drive the buffer to circulate in the liver perfusion system 10 through one output tube 34 and one peristaltic pump 40. If there is no third joint 35, then two input tubes 31, two output tubes 34 and two peristaltic pumps 40 are required, which will result in excessively high costs.

[0063] The embodiments of the utility model are described in detail above in conjunction with the accompanying drawings, but the utility model is not limited to the above embodiments. Various changes can be made within the knowledge of ordinary technicians in the relevant technical field without departing from the purpose of the utility model. In addition, the embodiments of the utility model and the features in the embodiments can be combined with each other without conflict.

Claims

1. A liver perfusion system, characterized in that: include: An organ basin having a storage cavity for accommodating an organ; a first circulation pipeline, comprising a first output tube and a first perfusion tube, wherein one end of the first output tube is in communication with the organ basin, and the other end of the first output tube is in communication with the first perfusion tube, the first output tube is located outside the organ basin, the first perfusion tube is located in the organ basin, the first output tube is used for perfusion fluid to flow into the organ basin, and the first perfusion tube is used for perfusion fluid to flow out of the first output tube; a second circulation pipeline, comprising a second output tube and a second perfusion tube, wherein one end of the second output tube is in communication with the organ basin, and the other end of the second output tube is in communication with the second perfusion tube, the second output tube is located outside the organ basin, the second perfusion tube is located in the organ basin, the second output tube is used for the perfusion liquid to flow into the organ basin, and the second perfusion tube is used for the perfusion liquid to flow out of the second output tube; A first pipe joint, comprising a first opening, a second opening and a third opening which are connected to each other, wherein the first opening is connected to the first output pipe, the second opening is connected to the first perfusion pipe, and the third opening is used to connect to the detection device; The second pipe joint includes a fourth opening, a fifth opening and a sixth opening which are connected to each other, wherein the fourth opening is connected to the second output pipe, the fifth opening is connected to the second perfusion pipe, and the sixth opening is used to connect to the detection device.

2. The liver perfusion system according to claim 1, characterized in that: The liver perfusion system further includes a first oxygenator and a second oxygenator, wherein the first oxygenator is in communication with the first circulation line, and the second oxygenator is in communication with the second circulation line.

3. The liver perfusion system according to claim 1, characterized in that: The liver perfusion system further includes a first embolic filter and a second embolic filter, wherein the first embolic filter is in communication with the first circulation pipeline, and the second embolic filter is in communication with the second circulation pipeline.

4. The liver perfusion system according to claim 1, characterized in that: The liver perfusion system also includes a first driving member and a second driving member, the first driving member is connected to the first circulation pipeline, the second driving member is connected to the second circulation pipeline, the first driving member is used to drive the perfusion fluid to flow in the first circulation pipeline, and the second driving member is used to drive the perfusion fluid to flow in the second circulation pipeline.

5. The liver perfusion system according to claim 1, characterized in that: The liver perfusion system further includes a first blocking member, which is used to block the third opening.

6. The liver perfusion system according to claim 5, characterized in that: The liver perfusion system further includes a second blocking member, which is used to block the sixth opening.

7. The liver perfusion system according to claim 1, characterized in that: The liver perfusion system further comprises an upper cover, which is detachably connected to the organ basin and is used to seal the opening of the storage cavity.

8. The liver perfusion system according to claim 7, characterized in that: The organ basin comprises a main body and a protruding part, wherein the main body is provided with the storage cavity, the protruding part is connected to the main body and protrudes toward the upper cover, the upper cover is provided with a positioning hole, and the protruding part is arranged in the positioning hole.

9. A detection device for detecting the liver perfusion system according to any one of claims 1 to 8, characterized in that: The detection device comprises: A pipeline tool, comprising an input pipe and an output pipe connected to each other, wherein the input pipe is connected to the third opening and the sixth opening respectively, and one end of the output pipe is connected to the organ basin; A peristaltic pump is connected to the output pipe, and the peristaltic pump is used to drive the buffer solution in the pipeline tooling to flow.

10. The detection device according to claim 9, characterized in that: The input pipe includes a first section and a second section, the first section is connected to the third opening, the second section is connected to the sixth opening, the pipeline tooling also includes a third joint, the third joint includes a seventh opening, an eighth opening and a ninth opening that are connected to each other, the seventh opening is connected to the first section, the eighth opening is connected to the second section, and the ninth opening is connected to the output pipe.

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