Organ chip with multi-layer structure

Abstract

The organ chip comprises a chip body, a first culture chamber, a plurality of second culture chambers and a third culture chamber are arranged in one end of the chip body, one end of each second culture chamber is communicated with the first culture chamber through a first flow channel, and the other end of each third culture chamber is communicated with the second culture chamber through a second flow channel. The other end of the second culture chamber is communicated with the third culture chamber through a second flow channel, a liquid inlet flow channel is formed in one end of the first culture chamber, a liquid discharge flow channel is formed in one side of the third culture chamber, a heating layer is arranged in the chip body, and a heat preservation layer is arranged on the chip body outside the heating layer. The first culture chamber, the plurality of second culture chambers and the third culture chambers are arranged inside one end of the chip body, so that cells are firstly cultured in the first culture chamber, then enter the second culture chambers for mixed culture, and finally are gathered in the third culture chamber for secondary culture; and moreover, the structure is reasonable, and the mixed culture operation is convenient and rapid.

Description

Technical Field

[0001] The utility model relates to the technical field of organ chips, in particular to an organ chip with a multi-layer structure. Background Art

[0002] An organ chip is a microfluidic cell culture system manufactured through micromachining technology. It can culture cells in three dimensions outside the human body to simulate the organ functions in the human body. Organ chips have good application prospects in the fields of drug development and screening, disease model evaluation, and personalized medicine. Organ chip technology can precisely control the space and fluids within the chip, thereby building tissue and organ modules with bionic results within the chip. At present, researchers have developed a variety of organ chips, including: liver, intestines, lungs, blood vessels, blood-brain barrier (BBB), kidneys, etc. Therefore, the development of organ chip technology will be helpful in the fields of biomedicine and drug development.

[0003] In the prior art, an organ chip with a multi-layer composite structure and a method of using the same are disclosed in the application number 202020614725.0. The organ chip comprises at least three layers of composite structures from bottom to top, including a bottom base structure, a first intermediate layer of at least 1 and a top cover structure, which can be used for multi-organ cell inoculation, and the bottom and intermediate layers can be used for cell inoculation and short-term static culture separately. After the cells adhere and grow, the bottom layer, the intermediate layer, and the top cover are combined, and an independent culture chamber is formed between the layers. Each chamber can be dynamically perfused and cultured, and substances can be exchanged between each chamber through a porous membrane. Research work in the fields of biomedicine, new drug development, toxicology, etc. can be applied, but the mixed cell culture operation is inconvenient, and it is not convenient to heat the inside of the chip body, thereby affecting the efficiency of organ cell culture. Utility Model Content

[0004] The utility model aims to provide an organ chip with a multilayer structure to solve the problems in the prior art that mixed cell culture is inconvenient to operate and it is inconvenient to heat the inside of the chip, thereby affecting the efficiency of organ cell culture.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an organ chip with a multi-layer structure, comprising a chip body, wherein a first culture chamber, a plurality of second culture chambers and a third culture chamber are arranged inside one end of the chip body, and one end of a single second culture chamber is connected to the first culture chamber through a first flow channel, and the other end of the second culture chamber is connected to the third culture chamber through a second flow channel, a liquid inlet flow channel is arranged at one end of the first culture chamber, a liquid discharge flow channel is arranged at one side of the third culture chamber, a heating layer is arranged inside the chip body, and an insulation layer is arranged on the chip body outside the heating layer.

[0006] Furthermore, a first sealing plug is provided at one end of the liquid inlet channel, and a second sealing plug is provided at one end of the liquid discharge channel.

[0007] Furthermore, the first culture chamber, the plurality of second culture chambers and the third culture chamber are all configured as rectangular chambers, and the spatial capacities of the third culture chamber, the first culture chamber and the second culture chamber decrease in sequence.

[0008] Furthermore, the number of the second culture chambers is three or four, and the flow passages at both ends of the second culture chambers are respectively provided with a first stop valve and a second stop valve.

[0009] Furthermore, the heating layer uses heating rods, and a plurality of heating rods are arranged at equal intervals, and a temperature controller is also provided on one side of the heating layer.

[0010] Furthermore, the thermal insulation layer is a rubber layer, and the thermal insulation layer covers three external surfaces of the chip body.

[0011] Compared with the prior art, the beneficial effects of the utility model are:

[0012] 1. The utility model has a first culture chamber, a plurality of second culture chambers and a third culture chamber inside one end of the chip body, so that cells are first cultured through the first culture chamber, then mixed cultured in the second culture chamber, and finally gathered in the third culture chamber for further culture, so that the needs of joint culture of multiple organs can be met, and the structure is reasonable, and the mixed culture operation is convenient and fast.

[0013] 2. The utility model provides a heating layer inside the chip body. The heating layer adopts heating rods, and multiple heating rods are arranged at equal intervals. A temperature controller is also provided on one side of the heating layer, so that it is convenient to heat the inside of the chip body. The heating uniformity is high, which is beneficial to improve the efficiency of organ cell culture.

[0014] 3. The utility model provides a heat-insulating layer on the chip body that goes out through the heating layer. The heat-insulating layer is made of a rubber layer, and the heat-insulating layer covers three external surfaces of the chip body, so that the outside of the chip body can be heat-insulated. In addition, the heat-insulating layer has certain shock-absorbing properties, which is convenient for improving the safety of the chip body. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0017] Figure 2 It is a schematic diagram of the internal structure of the chip body of the utility model;

[0018] Figure 3 It is a cross-sectional view of the utility model at section AA.

[0019] In the figure: 1. chip body; 2. first culture chamber; 3. second culture chamber; 4. third culture chamber; 5. insulation layer; 6. first flow channel; 7. first stop valve; 8. second flow channel; 9. second stop valve; 10. liquid inlet flow channel; 11. first sealing plug; 12. liquid discharge flow channel; 13. second sealing plug; 14. heating layer. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] See also Figure 1 , Figure 2 , Figure 3 In an embodiment of the utility model, a multi-layered organ chip includes a chip body 1, wherein a first culture chamber 2, a plurality of second culture chambers 3 and a third culture chamber 4 are arranged inside one end of the chip body 1, and one end of a single second culture chamber 3 is connected to the first culture chamber 2 through a first flow channel 6, and the other end of the second culture chamber 3 is connected to the third culture chamber 4 through a second flow channel 8. Cells are first cultured through the first culture chamber 2, and then mixed cultured in the second culture chamber 3, and finally gathered in the third culture chamber 4 for re-culture, so that the needs of joint culture of multiple organs can be met, and the structure is reasonable, and the mixed culture operation is convenient. Convenient and fast, a liquid inlet channel 10 is provided at one end of the first culture chamber 2, a liquid discharge channel 12 is provided at one side of the third culture chamber 4, a heating layer 14 is provided inside the chip body 1, the heating layer 14 adopts a heating rod, and a plurality of heating rods are provided at equal intervals, and a temperature controller is also provided on one side of the heating layer 14, so that it is convenient to heat the inside of the chip body 1, and the heating uniformity is high, which is conducive to improving the efficiency of organ cell culture, and a heat preservation layer 5 is provided on the chip body 1 outside the heating layer 14, so that the outside of the chip body 1 can be heat-insulated, and the heat preservation layer 5 has certain shock-absorbing performance, which is convenient to improve the safety of the chip body 1. The heat preservation layer 5 adopts a rubber layer, and the heat preservation layer 5 covers the three external surfaces of the chip body 1, so that the heat preservation layer 5 has a high covering efficiency, which is convenient to improve the heat preservation and shock-absorbing performance of the heat preservation layer 5.

[0022] A first sealing plug 11 is provided at one end of the liquid inlet channel 10 , and a second sealing plug 13 is provided at one end of the liquid discharge channel 12 , so that the opening and closing of the liquid inlet channel 10 and the liquid discharge channel 12 can be easily controlled.

[0023] The first culture chamber 2, the plurality of second culture chambers 3 and the third culture chamber 4 are all arranged as rectangular chambers, and the space capacity of the third culture chamber 4, the first culture chamber 2 and the second culture chamber 3 is reduced successively, so as to meet the use and processing of cell culture of different capacities. The number of the second culture chambers 3 is three or four, and the flow channels at both ends of the second culture chamber 3 are respectively provided with a first stop valve 7 and a second stop valve 9, so as to facilitate the control of the opening and closing of the first flow channel 6 and the second flow channel 8, and the control is convenient and quick.

[0024] The working principle and use process of the utility model are as follows: when in use, a first culture chamber 2, multiple second culture chambers 3 and a third culture chamber 4 are provided inside one end of the chip body 1. Cells are first cultured through the first culture chamber 2, then enter the second culture chamber 3 for mixed culture, and finally are gathered in the third culture chamber 4 for re-culture, so that the needs of joint culture of multiple organs can be met, and the structure is reasonable, and the mixed culture operation is convenient and fast.

[0025] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. An organ chip with a multilayer structure, comprising a chip body (1), characterized in that: A first culture chamber (2), a plurality of second culture chambers (3) and a third culture chamber (4) are provided inside one end of the chip body (1), and one end of a single second culture chamber (3) is connected to the first culture chamber (2) via a first flow channel (6), and the other end of the second culture chamber (3) is connected to the third culture chamber (4) via a second flow channel (8). A liquid inlet flow channel (10) is provided at one end of the first culture chamber (2), and a liquid discharge flow channel (12) is provided on one side of the third culture chamber (4). A heating layer (14) is provided inside the chip body (1), and a heat preservation layer (5) is provided on the chip body (1) outside the heating layer (14).

2. The organ chip with a multilayer structure according to claim 1, characterized in that: A first sealing plug (11) is provided at one end of the liquid inlet channel (10), and a second sealing plug (13) is provided at one end of the liquid discharge channel (12).

3. The organ chip with a multilayer structure according to claim 1, characterized in that: The first culture chamber (2), the plurality of second culture chambers (3) and the third culture chamber (4) are all arranged as rectangular chambers, and the spatial capacities of the third culture chamber (4), the first culture chamber (2) and the second culture chamber (3) decrease in sequence.

4. The organ chip with a multilayer structure according to claim 3, characterized in that: The number of the second culture chambers (3) is three or four, and the flow passages at both ends of the second culture chambers (3) are respectively provided with a first stop valve (7) and a second stop valve (9).

5. The organ chip with a multi-layer structure according to claim 1, characterized in that: The heating layer (14) uses heating rods, and a plurality of heating rods are arranged at equal intervals. A temperature controller is also provided on one side of the heating layer (14).

6. The organ chip with a multi-layer structure according to claim 1, characterized in that: The thermal insulation layer (5) is a rubber layer, and the thermal insulation layer (5) covers three external surfaces of the chip body (1).

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