Membrane strip fixing and reacting device

Abstract

The utility model discloses a membrane strip fixing and reacting device and relates to the technical field of protein detection. The fixing device of the membrane strip comprises a reaction part and a fixing part, wherein the reaction part is used for placing the membrane strip and the fixing part, and the fixing part is arranged at one axial end of the reaction part and is used for fixing one end of the membrane strip; the reaction part comprises a blocking rod, and the blocking rod is arranged at the end away from the fixing part in the axial direction of the reaction part and used for fixing the other end of the film strip. The membrane strip is fixed in the reaction groove, so that the operation is simple and convenient, and meanwhile, the operation of sticking the membrane strip to the bottom of the reaction groove by using glue is omitted, and the operation convenience of a western blot experiment and the accuracy of a detection result are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein detection, in particular to a film strip fixing and reaction device. Background Art

[0002] Protein immunoblotting is an effective method for identifying antigens with the help of specific antibodies. This method is a new immunobiochemical technique developed on the basis of gel electrophoresis and solid phase immunoassay technology. Protein immunoblotting is to transfer the protein components separated by electrophoresis from the gel to a solid phase support, and detect them through the specific immune reaction between the antibody and the antigen epitope presented by the target protein attached to the solid phase support. The whole process includes four parts: protein electrophoresis separation, electrical transfer, immunological detection and signal detection.

[0003] At present, the immunodetection part of the existing protein blotting experiment includes the following processes: blocking, first antibody binding, washing, second antibody binding, washing. In this part of the experimental process, the transferred membrane strip is generally pasted to the bottom of the reaction tank first to prevent the membrane strip from moving in the reaction tank or floating to the surface of the reaction liquid during the incubation process after adding samples or reaction reagents, thereby affecting the antibody incubation. However, the operation of pasting the membrane strip to the bottom of the reaction tank will introduce glue, thereby affecting the incubation reaction, or contaminating the surface of the membrane strip, affecting the detection of the signal.

[0004] In view of this, this application is hereby filed. Utility Model Content

[0005] The utility model aims to provide a membrane strip fixing and reaction device, which can omit the operation step of using glue to stick the membrane strip to the bottom of the reaction groove, thereby improving the simplicity of operation of protein blotting experiments and the accuracy of detection results.

[0006] The embodiment of the utility model is achieved as follows:

[0007] In the first aspect, the utility model provides a fixing device for a membrane strip, which includes a reaction piece and a fixing piece; wherein the reaction piece is used to place the membrane strip and the fixing piece, the fixing piece is arranged at one axial end of the reaction piece, and is used to fix one end of the membrane strip; the reaction piece includes a gear rod, which is arranged along the axial direction of the reaction piece at one end away from the fixing piece, and is used to fix the other end of the membrane strip.

[0008] In an optional embodiment, the reaction piece includes a reaction groove and a fixing piece groove. The reaction groove is arranged along the axial direction of the reaction piece, and the reaction groove is used to place the membrane strip; the fixing piece groove is arranged at one axial end of the reaction groove away from the gear rod, and the fixing piece groove is used to place the fixing piece.

[0009] In an optional embodiment, the fixing member groove and the reaction groove are connected in the axial direction of the reaction member.

[0010] In an optional embodiment, one end of the membrane strip is placed between the stop bar and the bottom of the reaction groove; the other end of the membrane strip is placed along the reaction groove and extends to the fixing member groove.

[0011] In an optional embodiment, the reaction groove includes a side wall, and the side wall includes an axial side wall and a radial side wall; the stopper rod is arranged on the axial side wall and is arranged close to the bottom of the reaction groove.

[0012] In an optional embodiment, the gear lever includes a first gear lever and a second gear lever; the axial side wall includes a first axial side wall and a second axial side wall; the first gear lever and the second gear lever are respectively arranged on the inner sides of the first axial side wall and the second axial side wall.

[0013] In an optional embodiment, the first gear rod and the second gear rod are arranged opposite to each other at the same horizontal height, and the width between the first gear rod and the second gear rod is smaller than the width of the film strip.

[0014] In an optional embodiment, the fixing device of the membrane strip further comprises a handle; the handle is arranged along the axial direction of the reaction piece, and one end of the handle close to the fixing piece groove is connected to the outer side of the radial side wall.

[0015] In a second aspect, the utility model provides a membrane strip reaction device, which comprises a membrane strip fixing device and a membrane strip as described above.

[0016] In an optional embodiment, a membrane strip is installed in the reaction device.

[0017] The beneficial effects of the embodiments of the utility model are:

[0018] The utility model provides a membrane strip fixing and reaction device, which includes a reaction piece and a fixing piece; the fixing piece is arranged at one axial end of the reaction piece, and is used to fix one end of the membrane strip; the reaction piece includes a gear rod, which is arranged along the axial direction of the reaction piece at one end away from the fixing piece, and is used to fix the other end of the membrane strip. The arrangement of this structure can effectively prevent the membrane strip from moving in the reaction liquid, or floating on the surface of the reaction liquid to affect the smooth progress of antibody incubation. When the membrane strip is installed on the reaction piece, the operation step of using glue to stick the membrane strip to the bottom of the reaction piece is omitted, thereby avoiding the influence of glue on the incubation reaction and contaminating the surface of the membrane strip to affect the detection of signals.

[0019] In addition, the membrane strip fixing device provided by the utility model can also be used as a membrane strip reaction device, which can effectively improve the ease of operation of protein blotting experiments and the accuracy of detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 This is a schematic diagram of the structure of the reaction part of the embodiment of the utility model;

[0022] Figure 2 This is a schematic diagram of the installation process of the membrane strip in the embodiment of the utility model;

[0023] Figure 3 This is a flow chart of the installation of the membrane strips of the utility model embodiment;

[0024] Figure 4 This is a flow chart of the protein immunoblotting method of the present utility model embodiment;

[0025] Figure 5 The utility model is a flow chart of the reaction device of the membrane strip.

[0026] Icons: 100 - fixing device of membrane strip; 111 - handle; 200 - reaction piece; 201 - fixing piece groove; 2011 - fixing piece; 202 - reaction groove; 203 - gear lever; 2031 - first gear lever; 2032 - second gear lever; 210 - axial side wall; 211 - first axial side wall; 212 - second axial side wall; 220 - radial side wall; 221 - first radial side wall; 222 - second radial side wall; 300 - membrane strip; 400 - schematic diagram of installing membrane strip. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0028] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the present invention to be protected, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0030] In the description of the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate the orientation or position relationship based on the orientation or position relationship shown in the accompanying drawings, or the orientation or position relationship in which the utility model product is usually placed when in use, which is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0031] In addition, the terms "horizontal", "vertical" and the like do not mean that the components are required to be absolutely horizontal or suspended, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0032] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0033] First embodiment

[0034] Please refer to Figure 1 and Figure 2 The present embodiment provides a membrane strip fixing device 100, which includes a reaction piece 200 and a cover piece (not shown in the figure). The cover piece can be detachably installed on the top of the reaction piece 200. When the membrane strip 300 is fixedly installed, or a reaction liquid is added to the membrane strip fixing device 100, the cover piece can be installed and placed on the top of the reaction piece 200 to prevent the membrane strip 300 from being contaminated by contact with the air, or the reaction liquid from reacting with the air to affect the accuracy of the detection result.

[0035] It should be noted that, in the present embodiment, the connection method between the cover plate member and the reaction member 200 may be a snap connection method, and other connection methods may be selected in other embodiments of the present invention according to actual needs.

[0036] In this embodiment, the reaction piece 200 includes a reaction groove 202 and a fixing piece groove 201. The reaction groove 202 is arranged along the axial direction of the reaction piece 200, and the reaction groove 202 is used to place the membrane strip 300; the fixing piece groove 201 is connected to the reaction groove 202 and is arranged at one axial end of the reaction piece 200, and the fixing piece groove 201 is used to place the fixing piece 2011 and fix one end of the membrane strip 300.

[0037] In this embodiment, the reaction groove 202 and the fixing member groove 201 are independently arranged and communicated with each other. In other embodiments of the present utility model, the reaction groove 202 and the fixing member groove 201 can be reasonably arranged according to actual needs.

[0038] In this embodiment, the membrane strip 300 is made of nitrocellulose (NC) membrane or polyvinylidene fluoride (PVDF) membrane. In other embodiments of the present invention, the membrane strip 300 can be reasonably selected according to actual needs.

[0039] It should be noted that the size of the membrane strip 300 is limited by the specifications of the reaction piece 200. It can be reasonably cut according to actual needs so that when the membrane strip 300 is placed in the reaction piece 200 for antibody incubation, it will not move or drift due to the increase of reaction liquid, thereby affecting the detection results.

[0040] The fixing member slot 201 is matched with the fixing member 2011, that is, when the fixing member 2011 is installed in the fixing member slot 201, it is in contact with the surrounding of the fixing member slot 201. The structure of the fixing member slot 201 and the fixing member 2011 is not particularly limited in the present invention, and can be reasonably set and adjusted according to actual needs. Specifically in this embodiment, the fixing member slot 201 is a cylindrical groove, and the fixing member 2011 is a cylindrical structure.

[0041] It should be noted that the bottom of the fixing groove 201 and the bottom of the fixing member 2011 are both flat, ensuring the flatness of the membrane strip 300 when installed in the reaction member 200, and preventing the distortion of the membrane strip 300 from affecting the detection result.

[0042] In this embodiment, the material used to make the fixing member 2011 is a soft rubber material, such as silicone rubber, TPE, etc. The use of soft rubber materials can prevent the membrane strip 300 from being damaged during the reaction process, ensure the smooth progress of the reaction, and provide effective protection for the accuracy of the test results. In other embodiments of the utility model, the material used to make the fixing member 2011 can be reasonably selected according to actual needs.

[0043] In this embodiment, after the fixing member 2011 is placed in the fixing member slot 201, it is higher than the top of the fixing member slot 201, that is, the vertical height of the fixing member 2011 is greater than the vertical height of the fixing member slot 201, which is convenient for operators to install or remove the fixing member 2011.

[0044] It should be noted that in other embodiments of the present invention, the vertical height of the fixing member 2011 can be set equal to the vertical height of the fixing member slot 201, or the vertical height of the fixing member 2011 can be set lower than the vertical height of the fixing member slot 201. In this structure, a protrusion that is higher than the vertical height of the fixing member slot 201 needs to be set at the center of the fixing member 2011. The setting of the protrusion facilitates the operator to install or remove the fixing member 2011. The present invention does not limit the structure of the protrusion, and it can be reasonably adjusted and set according to actual needs.

[0045] In the present invention, the reaction groove 202 is generally made of a material resistant to organic solvents or acid and alkali corrosion. The present invention does not limit the manufacturing material. Specifically, in this embodiment, the material used to prepare the reaction groove 202 is a plastic material, such as PP, ABS, etc. In addition, the reaction piece 200 can be reused or disposable. When it needs to be reused, the prepared material also needs to have the characteristic of being easy to clean.

[0046] Further, the reaction groove 202 includes a side wall, and the side wall includes an axial side wall 210 and a radial side wall 220, wherein the axial side wall 210 includes a first axial side wall 211 and a second axial side wall 212, and the first axial side wall 211 and the second axial side wall 212 are arranged opposite to each other in a form of being narrow at the bottom and wide at the top. One end of the axial side wall 210 is close to the fixing groove 201, and the other end of the axial side wall 210 is connected to the radial side wall 220, and the axial side wall 210 is provided with a stop rod 203 at one end away from the fixing groove 201.

[0047] The radial side wall 220 includes a first radial side wall 221 and a second radial side wall 222. The first radial side wall 221 is arranged near one end of the gear rod 203 and is directly connected to the axial side wall 210. The second radial side wall 222 is arranged near one end of the fixing member groove 201, and a groove with a width greater than the width of the membrane strip 300 is opened on the second radial side wall 222. When the membrane strip 300 is installed and placed in the reaction member 200, one end of the membrane strip 300 can extend along the reaction groove 202 through the groove opened in the second radial side wall 222 and be placed in the fixing member groove 201.

[0048] Specifically, in this embodiment, the fixing member groove 201 and the reaction groove 202 are connected together through the groove formed by the second radial side wall 222. When the membrane strip 300 is installed in the reaction member 200, one end of the membrane strip 300 can be extended and placed in the fixing member groove 201, and the fixing member 2011 is placed in the fixing member groove 201 to fix one end of the membrane strip 300.

[0049] The reaction piece 200 further includes a handle 111. Specifically, the handle 111 is disposed on the outer side of the fixing piece slot 201 away from the second radial side wall 222, so as to facilitate an operator to hold or transfer the film strip fixing device 100.

[0050] It should be noted that the length of the handle 111 needs to be set according to the convenience of the operator, and the width of the handle 111 is not particularly limited in the present invention. In this embodiment, the width of the handle 111 is equal to the horizontal distance between the top of the first axial side wall 211 and the top of the second axial side wall 212. In other embodiments of the present invention, the width of the handle 111 can be reasonably adjusted and set according to actual needs.

[0051] A lever 203 is disposed inside the reaction groove 202 and is disposed on the axial sidewall 210. Specifically, the lever 203 is disposed at one end of the axial sidewall 210 away from the fixing slot 201, that is, the lever 203 is disposed at one end close to the first radial sidewall 221 for fixing one end of the membrane strip 300.

[0052] For further information, please refer to Figure 1 The gear rod 203 includes a first gear rod 2031 and a second gear rod 2032, which are respectively arranged on the inner side of the first axial side wall 211 and the second axial side wall 212. In this embodiment, the first gear rod 2031 and the second gear rod 2032 are arranged opposite to each other at the same horizontal height, and the width between the first gear rod 2031 and the second gear rod 2032 is smaller than the width of the membrane strip 300, and the two are not connected, leaving a gap, and this structural setting is more conducive to the smooth flow of the injected reaction liquid in the reaction groove 202. The setting of the gear rod 203 can prevent the membrane strip 300 from floating in the reaction liquid and moving during the reaction process, thereby affecting the smooth progress of the incubation reaction, or contaminating the surface of the membrane strip 300, affecting the detection of the signal.

[0053] Furthermore, the position of the lever 203 disposed on the side wall of the reaction groove 202, that is, the vertical height of the lever 203 is greater than the thickness of the film strip 300, so that the film strip 300 can be smoothly inserted into or pulled out of the reaction groove 202. The structure of the lever 203 is not limited in the present invention. Specifically, in this embodiment, because one end of the lever 203 is abutted against the inner side of the axial side wall 210 of the reaction groove 202, the cross section of the lever 203 along the axial direction of the reaction groove 202 is a trapezoid; the cross section of the other end of the lever 203 opposite to the axial side wall 210 is a square. In other embodiments of the present invention, the structure of the lever 203 can be reasonably adjusted and set according to actual needs.

[0054] In this embodiment, the cover plate is matched with the reaction member 200. Specifically, the cover plate extends from the first radial side wall 221 of the reaction groove 202 to the handle 111. When the cover plate is installed on the reaction member 200, it abuts against the reaction member 200 and is sealed with the reaction member 200 to prevent the reaction liquid from spilling. In this embodiment, the cover plate is provided with a convex portion corresponding to the vertical height of the fixing member 2011 at a position corresponding to the fixing member groove 201 to ensure the sealing of the entire device.

[0055] Please refer to Figure 2 Installation diagram of membrane strip 400 and Figure 3 The flow chart for installing the membrane strip has the following assembly steps: the first step is to insert one end of the membrane strip 300 evenly under the gear lever 203 of the reaction groove 202; the second step is to always keep the membrane strip 300 flat during the process of inserting the membrane strip 300 into the reaction groove 202, so that one end of the membrane strip 300 is finally abutted against the first radial side wall 221 of the reaction groove 202, and the other end of the membrane strip 300 is placed along the axial direction of the reaction groove 202 and extends to the fixing groove 201; the third step is to insert the fixing 2011 into the fixing groove 201 and press the membrane strip 300.

[0056] It should be noted that in this embodiment, during the installation process, the membrane strip 300 does not need to be glued and fixed, the operation steps are simple, and inaccurate detection results caused by the reaction liquid being contaminated by glue can be effectively avoided.

[0057] Second embodiment

[0058] This embodiment provides a reaction device for a membrane strip 300, which includes the membrane strip fixing device 100 and the membrane strip 300 as described in the first embodiment. The membrane strip 300 has been installed and fixed at the bottom of the reaction groove 202, and there is no need to use glue to bond the membrane strip 300, nor to install the membrane strip 300 separately, and samples and antibodies can be directly added for incubation.

[0059] Please refer to Figure 4 and Figure 5 , provides a specific operation process of using the reaction device using the membrane strip in the utility model. This operation avoids the use of glue to stick the membrane strip 300 to the bottom of the reaction groove 202, which causes the incubation reaction to fail to proceed smoothly, and effectively improves the accuracy of the detection result.

[0060] In summary, the utility model provides a film strip fixing device 100, which can also be used as a reaction device for the film strip 300. One end of the film strip 300 is clamped by the lever 203 of the reaction groove 202, and the other end is pressed by the fixing member 2011. This can prevent the film strip 300 from floating to the surface of the reaction liquid and being contaminated when the reaction liquid is added to the reaction groove 202, and affect the smooth progress of the antibody incubation. This fixing method does not require the use of glue to stick the film strip 300, which simplifies the inspection operation process. No glue is needed during the installation of the film strip 300, which avoids the risk of the reaction liquid being contaminated by glue during the reaction process and the risk of affecting the detection.

[0061] The above description is only the preferred embodiment of the utility model, and is not intended to limit the utility model. For those skilled in the art, the utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A film strip fixing device, characterized in that: The fixing device of the membrane strip comprises a reaction piece and a fixing piece; The reaction piece is used to place the film strip and the fixing piece, and the fixing piece is arranged at one axial end of the reaction piece and is used to fix one end of the film strip; The reaction piece comprises a stopper rod, which is arranged at one end away from the fixing piece along the axial direction of the reaction piece and is used to fix the other end of the membrane strip.

2. The film strip fixing device according to claim 1, characterized in that: The reaction piece comprises a reaction groove and a fixing piece groove, wherein the reaction groove is arranged along the axial direction of the reaction piece and is used for placing the membrane strip; The fixing member groove is arranged at one axial end of the reaction groove away from the gear lever, and the fixing member groove is used for placing the fixing member.

3. The film strip fixing device according to claim 2, characterized in that: The fixing member groove is connected to the reaction groove in the axial direction of the reaction member.

4. The film strip fixing device according to claim 3, characterized in that: One end of the membrane strip is placed between the stop bar and the bottom of the reaction groove; the other end of the membrane strip is placed along the reaction groove and extends to the fixing member groove.

5. The film strip fixing device according to claim 2, characterized in that: The reaction groove comprises a side wall, and the side wall comprises an axial side wall and a radial side wall; The stop rod is arranged on the axial side wall and close to the bottom of the reaction groove.

6. The film strip fixing device according to claim 5, characterized in that: The gear lever comprises a first gear lever and a second gear lever; the axial side wall comprises a first axial side wall and a second axial side wall; The first gear lever and the second gear lever are respectively disposed on inner sides of the first axial side wall and the second axial side wall.

7. The film strip fixing device according to claim 6, characterized in that: The first gear rod and the second gear rod are arranged opposite to each other at the same horizontal height, and the width between the first gear rod and the second gear rod is smaller than the width of the film strip.

8. The film strip fixing device according to claim 2, characterized in that: The film strip fixing device also includes a handle; The handle is arranged along the axial direction of the reaction piece, and one end of the handle close to the fixing piece groove is connected to the outer side of the radial side wall.

9. A membrane strip reaction device, characterized in that: The reaction device comprises the fixing device and the membrane strip as described in any one of claims 1 to 8.

10. The membrane strip reaction device according to claim 9, characterized in that: A membrane strip is installed in the reaction device.

Images