Ultralow-temperature preservation containing box for sperms of yellow lip fish

Abstract

The ultralow-temperature preservation containing box comprises an ultralow-temperature freezing box, an air outlet is formed in the surface, close to the bottom, of the ultralow-temperature freezing box, a box door is rotationally installed on the front face of the ultralow-temperature freezing box through a hinge, and installation plates are fixed in the ultralow-temperature freezing box at equal intervals; a containing frame is slidably connected to the surface of the mounting plate in an inserted mode, the containing frame can be mounted on the mounting plate in an inserted mode along the inserting grooves through the two sides of the containing frame, and therefore the containing frame and the storage pipe can be conveniently placed in or taken out of the ultralow-temperature freezing box. After the ultra-low-temperature freezing box is sealed, the top of the storage pipe is opened through cooperation of the liquid injection assembly and the sealing assembly, a proper amount of diluent is added into the storage pipe, the good storage effect of the sperm of the yellow lip fish is guaranteed, manual operation is replaced, and more convenience and rapidness are achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of ultra-low temperature preservation, in particular to an ultra-low temperature preservation box for yellow lip fish sperm. Background Art

[0002] The yellow croaker is endemic to China. It is a rare and precious fish and the largest species of the Sciaenidae family. Due to overfishing, the number of yellow croakers has been declining and the fish body has gradually become smaller. It is now extremely rare and on the verge of extinction. In 2005, Dongguan City, Guangdong Province, China established a municipal nature reserve for yellow croakers to carry out artificial breeding. When artificially breeding yellow croakers, it is necessary to collect their fertilized eggs and sperm, and artificially intervene to form embryos. After the semen is collected, it needs to be placed in a freezer for long-term storage.

[0003] Conventional freezers, such as patent CN218511266U, propose an ultra-low temperature preservation box, wherein the placement mechanism includes multiple support rods, a top cover and multiple placement trays, the multiple support rods are fixedly mounted on the top of the annular lifting plate, the top cover is fixedly mounted on the top of the multiple support rods, and the multiple placement trays are fixedly sleeved on the multiple support rods. Items to be stored at low temperatures can be classified and placed through the multiple placement trays.

[0004] The shortcomings of the above scheme are: because fish semen needs to be stored in storage tubes, and in order to maintain the activity of sperm, diluent needs to be added to the storage tube before long-term frozen storage to achieve better frozen storage effect, and conventional freezers need to manually add diluent to each storage tube, which takes a lot of time and manpower, resulting in low efficiency in the collection and frozen storage of yellow croaker semen. Utility Model Content

[0005] In view of the shortcomings of the prior art, the utility model aims to provide a yellow lip fish sperm ultra-low temperature storage box to solve the problems raised in the above-mentioned background technology. The utility model has a novel structure. A storage tube filled with yellow lip fish semen is placed on a placement frame and sent into a mounting plate. After the ultra-low temperature freezer is sealed, the top of the storage tube is opened through the cooperation of the injection component and the sealing component, and an appropriate amount of diluent is added into the interior to ensure a good storage effect of the yellow lip fish semen. It also replaces manual operation and is more convenient.

[0006] In order to achieve the above-mentioned purpose, the utility model is realized through the following technical scheme: a yellow croaker sperm ultra-low temperature storage storage box, including an ultra-low temperature freezer, an air outlet is opened on the surface of the ultra-low temperature freezer near the bottom, and a box door is installed on the front of the ultra-low temperature freezer by hinge rotation, a mounting plate is fixed equidistantly inside the ultra-low temperature freezer, a placement frame is slidably plugged on the surface of the mounting plate, and storage tubes are placed in rows equidistantly plugged on the surface of the placement frame, a cover plate is provided on the top of the placement frame, and the cover plate covers the top of the storage tube, a liquid injection assembly is provided on the inner wall of the top of each layer of the placement frame corresponding to the ultra-low temperature freezer, and sealing assemblies for driving the cover plate to move up and down are provided on both sides of the front end of the mounting plate.

[0007] Furthermore, the injection assembly includes a movable plate, a syringe is fixed on the surface of the movable plate at a position corresponding to the storage tube, a sliding rod is fixed on the inner wall of the ultra-low temperature freezer on one side of the movable plate, and the movable plate is slidably sleeved on the sliding rod, a screw is rotatably installed on the inner wall of the ultra-low temperature freezer on the other side of the movable plate through a bearing, and the other end of the movable plate is meshingly sleeved on the screw, a motor is fixed on the outer wall of the ultra-low temperature freezer at a position corresponding to the screw, and the output end of the motor is fixed to the screw.

[0008] Furthermore, a diluent storage tank is fixed on the outer wall of the ultra-low temperature freezer away from the motor, and the diluent storage tank is connected to the syringe through a hose.

[0009] Furthermore, a plug hole is provided on the lower surface of the cover plate corresponding to the opening of the storage tube, and an elastic rubber sleeve is provided on the periphery of the plug hole, and the top of the storage tube is inserted into the plug hole.

[0010] Furthermore, the sealing assembly includes an electric push rod, and the electric push rods are fixed on both sides of the front end of the mounting plate, and the extended ends of the electric push rods are in compression contact with the cover plate.

[0011] Furthermore, telescopic rods are fixed on both sides of the front end of the placement frame, and the top of the telescopic rods is fixed to the cover plate. A spring is sleeved on the surface of the telescopic rod, and both ends of the spring are fixed to the cover plate and the placement frame.

[0012] Furthermore, the installation plate is provided with slots on both sides corresponding to the placement frame, and the placement frame is slidably plugged onto the surface of the installation plate through the slots.

[0013] The utility model has the following beneficial effects: the utility model provides a yellow croaker sperm ultra-low temperature storage box, comprising an ultra-low temperature freezer; a diluent storage box; a mounting plate; a placement frame; a storage tube; a slot; a liquid injection assembly; a slide bar; a motor; a screw rod; a moving plate; a syringe; a sealing assembly; an electric push rod; a telescopic rod; a spring; a cover plate; a jack;

[0014] 1. The utility model can be installed on the mounting plate by inserting and plugging the two sides of the placement frame along the slots, so that the placement frame and the storage tube can be easily put into or taken out of the ultra-low temperature freezer.

[0015] 2. In the utility model, under the action of the spring, the cover plate covers the opening of the storage tube and is sealed by an elastic rubber sleeve. When it is necessary to add diluent, the electric push rod lifts the top cover, the telescopic rod extends, the spring stretches, and the cover plate moves beyond the moving plate. The positions of the electric push rod and the telescopic rod are both located on the outside of the sliding rod, so they will not interfere with the translation of the moving plate.

[0016] 3. Compared with the prior art, the utility model is that the storage tube filled with yellow lip fish semen is placed on the placement frame and sent into the mounting plate. After the ultra-low temperature freezer is sealed, the top of the storage tube is opened through the cooperation of the injection component and the sealing component, and an appropriate amount of diluent is added into the inside to ensure a good storage effect of the yellow lip fish semen, and replaces manual operation, which is more convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the overall structure of a box for storing yellow croaker sperm at ultra-low temperature for storage according to the utility model;

[0018] Figure 2 This is a schematic diagram of the structure of a liquid injection component of a box for storing yellow croaker sperm at ultra-low temperature for storage according to the utility model;

[0019] Figure 3 This is a schematic diagram of the sealing component structure of a box for storing yellow croaker sperm at ultra-low temperature according to the utility model;

[0020] Figure 4 The utility model is a schematic diagram of the connection between a placement frame and a mounting plate of a yellow croaker sperm ultra-low temperature storage box.

[0021] In the figure: 1. Ultra-low temperature freezer; 2. Dilution storage box; 3. Mounting plate; 31. Placement frame; 32. Storage tube; 33. Slot; 4. Liquid injection assembly; 41. Slide rod; 42. Motor; 43. Screw; 44. Moving plate; 45. Syringe; 5. Sealing assembly; 51. Electric push rod; 52. Telescopic rod; 53. Spring; 54. Cover plate; 55. Socket. DETAILED DESCRIPTION

[0022] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.

[0023] See also Figures 1 to 4The utility model provides a technical solution: a yellow lip fish sperm ultra-low temperature storage box, comprising an ultra-low temperature freezer 1, an air outlet is provided on the surface of the ultra-low temperature freezer 1 near the bottom, and a box door is installed on the front of the ultra-low temperature freezer 1 through a hinge, a mounting plate 3 is fixed equidistantly inside the ultra-low temperature freezer 1, a placement frame 31 is slidably inserted on the surface of the mounting plate 3, and storage tubes 32 are inserted and placed in rows and equidistantly on the surface of the placement frame 31, a cover plate 54 is provided on the top of the placement frame 31, and the cover plate 54 covers the top of the storage tube 32, and the ultra-low temperature freezer 1 is provided with a cover plate 54. The ultra-low temperature freezer 1 is provided with a cover plate 54 on the top of the storage tube 32. The low-temperature freezer 1 is provided with a liquid injection component 4 on the inner wall corresponding to the top of each layer of the placement frame 31, and the two sides of the front end of the mounting plate 3 are provided with a sealing component 5 that drives the cover plate 54 to move up and down. The ultra-low temperature freezer 1 in the scheme technology is the existing technology. When using the device, the storage tube 32 containing yellow lip fish semen is placed in the placement frame 31 and sealed by the cover plate 54, and then placed on the mounting plate 3. After closing the box door, the liquid injection component 4 and the sealing component 5 are cooperated to open the cover plate 54, and the diluent is added to the storage tube 32, and then it is sealed and frozen for storage through the cover plate 54.

[0024] In this embodiment, the injection assembly 4 includes a movable plate 44, a syringe 45 is fixed on the surface of the movable plate 44 at a position corresponding to the storage tube 32, a slide bar 41 is fixed on the inner wall of the ultra-low temperature freezer 1 on one side of the movable plate 44, and the movable plate 44 is slidably sleeved on the slide bar 41, a screw 43 is rotatably installed on the inner wall of the ultra-low temperature freezer 1 on the other side of the movable plate 44 through a bearing, and the other end of the movable plate 44 is meshingly sleeved on the screw 43, a motor 42 is fixed on the outer wall of the ultra-low temperature freezer 1 at a position corresponding to the screw 43, and the output end of the motor 42 is fixed to the screw 43, a diluent storage tank 2 is fixed on the outer wall of the ultra-low temperature freezer 1 away from the motor 42, and the diluent storage tank 2 is connected to the syringe 45 through a hose, after the storage tube 32 is opened, the motor 42 is turned on to drive the screw 43 to rotate, the movable plate 44 meshes with the screw 43 and slides along the slide bar 41, and the injection After the bottom of the ejector 45 corresponds to the storage tube 32, the diluent is sent into the storage tube 32 by opening the built-in output pump and the electromagnetic valve, and is connected to the diluent storage box 2 through a hose, so that the diluent can be added to a row of storage tubes 32 at the same time (the preparation of the diluent is a key step in the cryopreservation of fish semen. Due to the specificity of fish species, the requirements for diluents for cryopreservation of semen of different fish are different, and there is no unified standard at present. It is generally believed that the composition of the diluent is as simple as possible within an appropriate range. The components of the used fish semen preservation diluent mainly include: salts (such as NaHCO3, NaCl, KC, etc.), sugars (such as glucose, fructose, sucrose, etc.), lipids (mainly phospholipids) and proteins (such as egg yolk, milk, calf serum, etc.) and other additives (such as vitamins, antibiotics, etc.). The diluent here can be a diluent specifically suitable for cryopreservation of yellow lip fish sperm).

[0025] In this embodiment, the cover plate 54 is provided with a plug hole 55 on the lower surface corresponding to the opening of the storage tube 32, and an elastic rubber sleeve is provided on the periphery of the plug hole 55, and the top of the storage tube 32 is inserted into the plug hole 55, and the sealing assembly 5 includes an electric push rod 51, and the electric push rod 51 is fixed on both sides of the front end of the mounting plate 3, and the extended end of the electric push rod 51 is pressed and contacted with the cover plate 54, and the two sides of the front end of the placement frame 31 are fixed with a telescopic rod 52, and the top of the telescopic rod 52 is fixed to the cover plate 54, and the surface of the telescopic rod 52 is sleeved. There is a spring 53, and both ends of the spring 53 are fixed to the cover plate 54 and the placement frame 31. The electric push rod 51 and the cover plate 54 are in extrusion contact. Under the action of the spring 53, the cover plate 54 covers the opening of the storage tube 32 and is sealed by an elastic rubber sleeve. When it is necessary to add diluent, the electric push rod 51 lifts the top cover, the telescopic rod 52 extends, the spring 53 stretches, and the cover plate 54 moves to exceed the movable plate 44. The positions of the electric push rod 51 and the telescopic rod 52 are both located on the outside of the sliding rod 41, so they will not interfere with the translation of the movable plate 44.

[0026] In this embodiment, slots 33 are provided on both sides of the mounting plate 3 corresponding to the placement frame 31, and the placement frame 31 is slidably plugged onto the surface of the mounting plate 3 through the slots 33. Both sides of the placement frame 31 can be plugged and installed on the mounting plate 3 along the slots 33, thereby facilitating the placement frame 31 and the storage tube 32 to be placed in or taken out of the ultra-low temperature freezer 1.

[0027] When using the device, the storage tube 32 containing yellow lip fish semen is placed in the placement frame 31 and sealed by the cover plate 54, and then placed on the mounting plate 3. After closing the box door, when it is necessary to add diluent, the electric push rod 51 lifts the top cover, the telescopic rod 52 extends, and the spring 53 stretches. After the storage tube 32 is opened, the motor 42 is turned on to drive the screw 43 to rotate, the moving plate 44 engages with the screw 43 and slides along the slide bar 41. After the bottom of the syringe 45 corresponds to the storage tube 32, the diluent is sent into the storage tube 32 by opening the built-in output pump and the solenoid valve, and then it is sealed and frozen for storage through the cover plate 54.

[0028] The above shows and describes the basic principles and main features of the utility model and the advantages of the utility model. For those skilled in the art, it is obvious that the utility model is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or basic features of the utility model. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the utility model is defined by the attached claims rather than the above description, and it is intended to include all changes within the meaning and scope of the equivalent elements of the claims. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0029] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A box for storing yellow croaker sperm at ultra-low temperature, comprising an ultra-low temperature freezer (1), characterized in that: The ultra-low temperature freezer (1) is provided with an air outlet on the surface near the bottom, and a door is rotatably installed on the front of the ultra-low temperature freezer (1) by means of hinges. A mounting plate (3) is fixed equidistantly inside the ultra-low temperature freezer (1). A placement frame (31) is slidably inserted on the surface of the mounting plate (3), and storage tubes (32) are inserted and placed in rows equidistantly on the surface of the placement frame (31). A cover plate (54) is provided on the top of the placement frame (31), and the cover plate (54) covers the top of the storage tube (32). A liquid injection assembly (4) is provided on the inner wall corresponding to the top of each layer of the placement frame (31) of the ultra-low temperature freezer (1), and sealing assemblies (5) for driving the cover plate (54) to move up and down are provided on both sides of the front end of the mounting plate (3).

2. The ultra-low temperature storage box for yellow croaker sperm according to claim 1, characterized in that: The injection assembly (4) comprises a movable plate (44), a syringe (45) is fixed on the surface of the movable plate (44) at a position corresponding to the storage tube (32), a slide bar (41) is fixed on the inner wall of the ultra-low temperature freezer (1) located on one side of the movable plate (44), and the movable plate (44) is slidably sleeved on the slide bar (41), a screw rod (43) is rotatably mounted on the inner wall of the ultra-low temperature freezer (1) located on the other side of the movable plate (44) through a bearing, and the other end of the movable plate (44) is meshingly sleeved on the screw rod (43), and a motor (42) is fixed on the outer wall of the ultra-low temperature freezer (1) at a position corresponding to the screw rod (43), and the output end of the motor (42) is fixed to the screw rod (43).

3. The ultra-low temperature storage box for yellow croaker sperm according to claim 2, characterized in that: A diluent storage box (2) is fixed on the outer wall of the ultra-low temperature freezer (1) at a side away from the motor (42), and the diluent storage box (2) is connected to the syringe (45) via a hose.

4. The ultra-low temperature storage box for yellow croaker sperm according to claim 1, characterized in that: A plug hole (55) is provided on the lower surface of the cover plate (54) corresponding to the opening of the storage tube (32), and an elastic rubber sleeve is provided on the periphery of the plug hole (55), and the top of the storage tube (32) is inserted into the plug hole (55).

5. The ultra-low temperature storage box for yellow croaker sperm according to claim 4, characterized in that: The sealing assembly (5) comprises an electric push rod (51), the electric push rods (51) are fixed on both sides of the front end of the mounting plate (3), and the extended ends of the electric push rods (51) are in compression contact with the cover plate (54).

6. The ultra-low temperature storage box for yellow croaker sperm according to claim 5, characterized in that: Telescopic rods (52) are fixed on both sides of the front end of the placement frame (31), and the top of the telescopic rod (52) is fixed to the cover plate (54). A spring (53) is sleeved on the surface of the telescopic rod (52), and the two ends of the spring (53) are fixed to the cover plate (54) and the placement frame (31).

7. The ultra-low temperature storage box for yellow croaker sperm according to claim 1, characterized in that: Slots (33) are provided on both sides of the mounting plate (3) corresponding to the placement frame (31), and the placement frame (31) is slidably plugged onto the surface of the mounting plate (3) through the slots (33).

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