Incubator capable of refrigerating / heating and uniformly mixing
By designing refrigeration/thermal mixed uniform incubation instruments, using semiconductor refrigeration tablets and servo motor -driven rotation devices and shake bed devices to solve the problem of stable low temperature or high temperature environment in antibody incubation experiments, realize the stability and accuracy of the experimental experiment , Avoid the effects of equipment life and refrigeration effects.
Patent Information
- Application Number
- CN202420854550.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-24
- Publication Date
- 2025-01-24
- Estimated Expiration
- 2034-04-24
AI Technical Summary
In the prior art, antibody incubation experiments are difficult to perform under stable low temperature or high temperature environments, especially in the Western blotting experiments with low temperature incubation overnight. The prior art cannot achieve stable low temperature requirements, which also affects the refrigerator refrigeration and shaker life. .
Design a refrigeration/thermal mixed uniform incubation instrument, using semiconductor refrigeration and power supply control system. The rotating device and horizontal shaking device driven by the servo motor can be achieved to achieve stable control of the inner cavity chamber. Temperature adjustment at 37 ℃.
It achieves the stability and accuracy of antibody incubation experiments under the conditions of 2~8℃ and 37℃, avoids the influence of refrigerator cooling effect and shaker life, while simplifying the operation process and improving the accuracy of the experimental environment.
Smart Images
Figure CN222393876U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of biological experimental equipment, in particular to the technical field of antibody incubation experimental equipment, and specifically is a refrigeration / heat mixing incubator. Background Art
[0002] Mixing and incubation is the most commonly used experimental method in biological experiments. Generally, the temperature of antibody incubation needs to be kept within a relatively constant range. For example, the overnight incubation of the most commonly used first antibody (primary antibody) in Western blotting experiments needs to be completed at 2~8℃. In some experiments, the incubation reaction of antigen and antibody needs to be carried out at 37℃, and the antibody needs to be mixed continuously while maintaining a constant temperature.
[0003] In the prior art, the primary antibody is incubated overnight at 2-8°C, which requires the shaker to be placed in a refrigerator at a specified temperature. However, the power cord of the shaker can only be extended from the gap of the refrigerator door to connect to the power supply, which makes it impossible to close the refrigerator tightly. In the long run, the sealing of the refrigerator is affected, and the refrigeration effect of the refrigerator is also affected. The ambient temperature of the experiment cannot be met, which affects the experimental effect. At the same time, there is a deviation between the operating temperature and the designed ambient temperature of the shaker. Long-term low-temperature operation also has an adverse effect on the life of the shaker.
[0004] For experiments that require mixing at 37°C, the actual operation in the prior art is usually carried out in a constant temperature water bath or controlled room temperature. The constant temperature effect is poor, and due to space limitations, the mixing requirement cannot be achieved in a 37°C constant temperature box. Such experiments can only be completed in a rotary mixer at room temperature, resulting in inaccurate temperature control, which ultimately affects the experimental results.
[0005] In addition, the thermostats on the market have a single temperature setting method. For example, most electric incubators used in experiments can only be set to a temperature higher than room temperature, and experiments that require mixing cannot be performed in them. Existing shakers only use fixed-direction shaking, and cannot adjust the direction angle and ambient temperature, which cannot conveniently meet experimental requirements. Therefore, it is necessary to provide a refrigeration / heat mixing incubator. Summary of the invention
[0006] The utility model provides a refrigeration / heat mixing incubator to solve the problems that the current electric heating incubator has a single temperature setting mode and can only set a temperature higher than the room temperature, and cannot implement experiments requiring mixing therein, especially the most commonly used primary antibody low-temperature incubation overnight experiment in Western blotting experiments. The existing technology cannot achieve the stable low-temperature requirement, and there is a problem that the refrigeration of the refrigerator and the life of the shaker are affected.
[0007] The utility model is implemented by the following technical solutions:
[0008] A refrigeration / heat mixing incubator comprises a box body, a semiconductor refrigeration sheet and a power supply control system for power supply are arranged on the back side of the box body, an inner chamber is arranged on the upper part of the box body, a rotating shaft is installed in the middle of the inner chamber through a rotating shaft bearing, one end of the rotating shaft passes through the inner chamber and is connected to a first servo motor controlled by a rotation control system, the first servo motor is installed in the box body, synchronously rotating fixed disks are installed at intervals on the rotating shaft, and test tube clamps are evenly distributed at intervals on the edge of the fixed disk; a shaking table is installed at the bottom of the inner chamber, a tray for placing culture dishes is arranged on the upper part of the shaking table, the four sides of the tray are connected to the cavity wall of the inner chamber through springs, the bottom bracket of the tray passes through the inner chamber and is connected to a second servo motor controlled by the shaking table control system, the second servo motor is installed in the box body, and operation buttons of the rotation control system, the shaking table control system and the power supply control system are arranged on a panel at the lower part of the box body.
[0009] During implementation, it includes a box body, a semiconductor refrigeration sheet and a power supply control system for power supply are provided on the back side of the box body, the semiconductor refrigeration sheet is used for adjusting the temperature of the inner chamber, the inner chamber is provided on the upper part of the box body, the semiconductor refrigeration sheet can provide a cold source or a heat source in the inner chamber, so as to realize the adjustment of the temperature of the inner chamber between low temperature (such as 2-8°C) and high temperature (such as 37°C), the cold end of the semiconductor refrigeration sheet faces the inner chamber, and a temperature sensor connected to the power supply control system is provided in the inner chamber, and the temperature of the inner chamber is fed back to the power supply control system through the temperature sensor, and then the power supply control system adjusts the semiconductor refrigeration sheet to control the internal temperature of the inner chamber, so that the actual temperature is stable within the set temperature range; the hot end of the semiconductor refrigeration sheet faces the inner surface of the box body, and the inner surface of the box body at the corresponding position is provided with heat dissipation holes for heat dissipation, and a DC fan for air circulation is provided in the inner chamber to ensure that the temperature at various places in the inner chamber is in a balanced state.
[0010] The front end of the inner chamber is provided with a door body hinged on the box body. The door body is sealed with the inner chamber. The surface of the door body is provided with a double-layer heat-insulating high borosilicate transparent glass for observing the experimental conditions in the inner chamber. The free end of the door body is provided with a rotating locking handle, and the lock of the handle cooperates with the box body.
[0011] A rotating shaft is installed in the middle of the inner chamber through a rotating shaft bearing, specifically, a rotating shaft bearing is clamped at the same position of the two side walls in the middle of the inner chamber, a rotating shaft is installed between the two rotating shaft bearings, and the rotating shaft is rotatably installed in the middle of the inner chamber, one end of the rotating shaft passes through the inner chamber and is driven and connected to a first servo motor controlled by a rotation control system, the first servo motor is installed in a box, and synchronously rotating fixed disks are installed at intervals on the rotating shaft, the fixed disk is in the shape of a disk, and test tube clamps are evenly spaced on the edge of the fixed disk, and the test tube clamps clamp the test tube horizontally; further, the fixed disk is installed separately, and the test tube clamp on each fixed disk is clamped with a test tube separately; as another way, the fixed disks are installed in pairs, and test tube clamps are provided at the same position of the two fixed disks in a pair, and the test tube clamps at the same position are respectively clamped with the head and tail of a test tube to prevent the test tube from rotating. The test tube clamp comprises a pair of elastic clamping plates arranged opposite to each other, the elastic clamping plates are made of metal sheets, the bottom end of each elastic clamping plate is mounted on a fixed plate, the elastic clamping plates can be mounted on the fixed plate by insertion or welding, and can also be detachably mounted by bolt connection in order to adapt to more test tube structures; the elastic clamping plate comprises a clamping section, after the test tube is installed in place, the two sides of the test tube are clamped in the clamping section, the clamping sections of the two pairs of elastic clamping plates are arranged in parallel to prevent the test tube from sliding outward, the top of the clamping section is integrally connected with an inclined locking section, the locking sections of the two pairs of elastic clamping plates are inclined inward, the test tube is clamped between the two elastic clamping plates, the top of the locking section is integrally connected with a handle section, the handle section is semicircular, the handle section is used to hold the elastic clamping plate open, and the edge of the arc of the handle section prevents the corners from piercing the outer wall of the test tube.
[0012] A shaker is installed at the bottom of the inner chamber, and a tray for placing culture dishes is provided on the upper part of the shaker. The four sides of the tray are connected to the cavity wall of the inner chamber through springs. The bottom bracket of the tray passes through the inner chamber and is connected to the second servo motor controlled by the shaker control system. The shaker adopts existing equipment and its structure is not described in detail. An insulating elastic gasket is provided at the bottom bracket passing through the inner chamber to keep the temperature of the inner chamber as constant as possible while ensuring the normal shaking of the shaker. The second servo motor is installed in the box, and the operation buttons of the rotation control system, the shaker control system and the power supply control system are arranged on the panel at the lower part of the box; the panel is provided with a first speed control knob connected to the rotation control system and a second speed control knob connected to the shaker control system, the first speed control knob controls the speed of the first servo motor, and the second speed control knob controls the speed of the second servo motor.
[0013] When in use, hold the handle to open the door and install the test tube on the test tube clamp. Specifically, hold the test tube in one hand and push the handle section with two fingers of the other hand to open the elastic clamp. At this time, the clamp section tilts outward, clamp the test tube into the clamp section and release the handle section. The inner wall of the clamp section clamps the outer wall of the test tube. The locking sections of the two elastic clamps wrap the test tube in the test tube clamp. Similarly, install the test tube clamp in the test tube clamp, rotate the shaft, and clamp all the test tubes. If the test tubes cannot fill the test tube clamps on the fixed plate during use, the test tubes should be installed symmetrically along the center of the fixed plate. The test tubes are installed. Place the culture dish firmly on the tray of the shaker, and the culture dish is installed.
[0014] Set the temperature. The temperature sensor in the inner chamber feeds back the temperature in the chamber to the power supply control system in real time. Then the power supply control system adjusts the semiconductor refrigeration plate and adjusts the temperature of the inner chamber to stabilize the actual temperature within the set temperature range. Adjust the first speed knob to control the speed and opening and closing of the first servo motor, thereby controlling the shaking of the test tube. Similarly, adjust the second speed knob to control the speed and opening and closing of the second servo motor, thereby controlling the shaking of the culture dish.
[0015] Compared with the prior art, the utility model has the following beneficial effects: the utility model provides a refrigeration / heating mixing incubator, which realizes stable temperature control in the instrument through semiconductor refrigeration / heating, such as 2~8℃ and 37℃ which are the most common temperatures in biological experiments. It can realize overnight incubation of Western blotting primary antibody at 2~8℃, and can also realize completion of mixing incubation reaction at 37℃.
[0016] The device is driven by a servo motor and is equipped with a rotating device and a horizontal shaking device. It can mix test tubes and culture dishes at the same time, and the two can be controlled separately. The speed of the rotating and horizontal shaking devices can be adjusted according to needs, which can meet the requirements of mixing various specimens.
[0017] The device can solve the problem that placing a shaker in a refrigerator affects the life of the shaker on the one hand and the refrigeration of the refrigerator on the other hand. It has a simple structure and is not prone to failure. At the same time, it can also free up refrigerator space in the laboratory and improve space utilization.
[0018] This device greatly simplifies the operation process, provides convenient conditions for the experiment, and improves the accuracy of the experimental environment. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 The utility model is represented by a sectional view of the overall structure.
[0020] Figure 2 The utility model is represented in its overall structure.
[0021] Figure 3 A side view of the present invention is shown.
[0022] Figure 4 A schematic diagram showing the structure of a test tube card.
[0023] In the figure: 1-box, 2-inner chamber, 3-rotating shaft bearing, 4-rotating shaft, 5-first servo motor, 6-fixed plate, 7-test tube clamp, 701-elastic clamping plate, 7001-clamping section, 7002-locking section, 7003-handle section, 8-shaking table, 801-tray, 802-spring, 803-bottom bracket, 9-second servo motor, 10-panel, 11-first speed control knob, 12-second speed control knob, 13-door body, 14-handle, 15-high borosilicate transparent glass, 16-heat dissipation hole. DETAILED DESCRIPTION
[0024] The specific embodiments of the present utility model are described below in conjunction with the accompanying drawings.
[0025] A refrigeration / heat mixing incubator, such as Figures 1 to 3 As shown: it includes a box body 1, a semiconductor refrigeration plate and a power supply control system for power supply are provided on the back side of the box body 1, the semiconductor refrigeration plate is used for adjusting the temperature of the inner chamber, an inner chamber 2 is provided on the upper part of the box body 1, the cold end of the semiconductor refrigeration plate faces the inner chamber 2, a temperature sensor connected to the power supply control system is provided in the inner chamber 2, the temperature of the inner chamber 2 is fed back to the power supply control system through the temperature sensor, and then the power supply control system adjusts the semiconductor refrigeration plate to control the internal temperature of the inner chamber, so that the actual temperature is stable within the set temperature range; the hot end of the semiconductor refrigeration plate faces the inner surface of the box body 1, and the inner surface of the box body 1 at the corresponding position is provided with heat dissipation holes 16 for heat dissipation, and a DC fan for air circulation is provided in the inner chamber 2 to ensure that the temperature at various locations in the inner chamber 2 is in a balanced state.
[0026] The front end of the inner chamber 2 is provided with a door body 13 hinged on the box body 1, and the door body 13 is sealed with the inner chamber 2. The surface of the door body 13 is provided with a double-layer heat-insulating borosilicate transparent glass 15 for observing the experimental conditions in the inner chamber 2. The free end of the door body 13 is provided with a rotating locking handle 14, and the lock of the handle 14 cooperates with the box body 1.
[0027] A rotating shaft 4 is installed in the middle of the inner chamber 2 through a rotating shaft bearing 3, specifically, a rotating shaft bearing 3 is clamped at the same position on both side walls of the middle of the inner chamber 2, a rotating shaft 4 is installed between the two rotating shaft bearings 3, the rotating shaft 4 is rotatably installed in the middle of the inner chamber 2, one end of the rotating shaft 4 passes through the inner chamber 2 and is driven and connected to a first servo motor 5 controlled by a rotation control system, the first servo motor 5 is installed in the housing 1, and synchronously rotating fixed disks 6 are installed at intervals on the rotating shaft 4, the fixed disk 6 is in the shape of a disk, and test tube clamps 7 are evenly spaced on the edge of the fixed disk 6, and the test tube clamps 7 clamp the test tube horizontally; in this embodiment, the fixed disks 6 are installed in pairs, and the test tube clamps 7 are provided at the same position of the two fixed disks 6 in a pair, and the test tube clamps 7 at the same position are respectively clamped at the head and tail of a test tube to prevent the test tube from being thrown off during rotation; the test tube clamp 7 includes a pair of elastic clamping plates 701 arranged opposite to each other, and the elastic clamping plates 701 are made of metal sheets, such as Figure 1 As shown: the bottom end of each elastic clamping plate 701 is installed on the fixed disk 6, and the elastic clamping plate 701 can be installed on the fixed disk 6 by insertion or welding. In order to adapt to more test tube structures, it can also be detachably installed by bolt connection; the elastic clamping plate 701 includes a clamping section 7001. After the test tube is installed in place, the two sides of the test tube are clamped in the clamping section 7001. The clamping sections 7001 of the two pairs of elastic clamping plates 701 are arranged in parallel to prevent the test tube from sliding outward. The top of the clamping section 7001 is integrally connected with an inclined locking section 7002. The locking sections 7002 of the two pairs of elastic clamping plates 701 are inclined inwardly to clamp the test tube between the two elastic clamping plates 701. The top of the locking section 7002 is integrally connected with a handle section 7003. The handle section 7003 is semicircular. The handle section 7003 is used to hold and open the elastic clamping plate 701. The edge of the arc of the handle section 7003 prevents the corners from piercing the outer wall of the test tube.
[0028] A shaker 8 is installed at the bottom of the inner chamber 2, and a tray 801 for placing a culture dish is provided on the upper part of the shaker 8. The four sides of the tray 801 are connected to the cavity wall of the inner chamber 2 through springs 802. The bottom bracket 803 of the tray 801 passes through the inner chamber 2 and is driven and connected to the second servo motor 9 controlled by the shaker control system. The bottom bracket 803 is provided with an insulating elastic gasket where it passes through the inner chamber 2, which ensures that the shaker swings normally while keeping the temperature of the inner chamber 2 as constant as possible. The second servo motor 9 is installed in the box 1, and the operation buttons of the rotation control system, the shaker control system and the power supply control system are arranged on the panel 10 at the lower part of the box; the panel 10 is provided with a first speed control knob 11 connected to the rotation control system and a second speed control knob 12 connected to the shaker control system, the first speed control knob 11 controls the rotation speed of the first servo motor 5, and the second speed control knob controls the rotation speed of the second servo motor 9.
[0029] When in use, the handle 14 is held to open the door 13, and the test tube is installed on the test tube clamp 7. Specifically, the test tube is held in one hand, and two fingers of the other hand push the clamping handle section 7003 to open the elastic clamping plate 701. At this time, the clamping section 7001 tilts outward, and the test tube is clamped into the clamping section 7001 and the clamping handle section 7003 is released. The inner wall of the clamping section 7001 clamps the outer wall of the test tube, and the locking sections 7002 of the two elastic clamping plates 701 wrap the test tube in the test tube clamp 7. Similarly, the test tube is clamped in the test tube clamp 7, and the rotating shaft 4 is rotated to clamp all the test tubes. If the test tubes cannot be fully filled in the test tube clamp 7 on the fixed plate 6 during use, the test tubes should be installed symmetrically along the center of the fixed plate 6; the test tube installation is completed. The culture dish is firmly placed on the tray 801 of the shaking table 8, and the culture dish installation is completed.
[0030] The temperature is set, and the temperature sensor in the inner chamber 2 feeds back the temperature in the chamber to the power supply control system in real time, and then the power supply control system adjusts the semiconductor refrigeration plate and adjusts the temperature of the inner chamber 2 to stabilize the actual temperature within the set temperature range. The first speed knob 11 is adjusted to control the speed and start and stop of the first servo motor 5, thereby controlling the shaking of the test tube. Similarly, the second speed knob 12 is adjusted to control the speed and start and stop of the second servo motor 9, thereby controlling the shaking of the culture dish.
[0031] The scope of protection claimed by the present invention is not limited to the above specific implementation methods. For those skilled in the art, the present invention may have various deformations and changes. Any modification, improvement and equivalent substitution made within the concept and principle of the present invention should be included in the protection scope of the present invention.
Claims
1. A refrigeration / heat mixing incubator, comprising a housing (1), wherein a semiconductor refrigeration sheet and a power supply control system for power supply are provided on the back side of the housing (1), characterized in that: An inner chamber (2) is provided at the upper part of the housing (1), a rotating shaft (4) is installed in the middle of the inner chamber (2) via a rotating shaft bearing (3), one end of the rotating shaft (4) passes through the inner chamber (2) and is drivingly connected to a first servo motor (5) controlled by a rotation control system, the first servo motor (5) is installed in the housing (1), and synchronously rotating fixed disks (6) are installed at intervals on the rotating shaft (4), and test tube clamps (7) are evenly distributed at intervals on the edge of the fixed disk (6); A shaking table (8) is installed at the bottom of the inner chamber (2); a tray (801) for placing a culture dish is provided on the upper part of the shaking table (8); four sides of the tray (801) are connected to the cavity wall of the inner chamber (2) via springs (802); a bottom bracket (803) of the tray (801) passes through the inner chamber (2) and is connected to a second servo motor (9) controlled by a shaking table control system; the second servo motor (9) is installed in the box (1); and operation buttons of the rotation control system, the shaking table control system and the power supply control system are arranged on a panel (10) at the lower part of the box.
2. A refrigeration / heat mixing incubator according to claim 1, characterized in that: The fixed disks (6) are installed in pairs, and test tube clamps (7) are provided at the same position of the two fixed disks (6) in the pair.
3. A refrigeration / heat mixing incubator according to claim 1, characterized in that: The test tube clamp (7) comprises a pair of elastic clamping plates (701) arranged facing each other, the bottom end of each elastic clamping plate (701) being mounted on a fixed plate (6), the elastic clamping plate (701) comprising a clamping section (7001), the top end of the clamping section (7001) being integrally connected to an inclined locking section (7002), and the top end of the locking section (7002) being integrally connected to a clamping handle section (7003).
4. A refrigeration / heat mixing incubator according to claim 3, characterized in that: The clamping sections (7001) of the two paired elastic clamping plates (701) are arranged in parallel, the locking sections (7002) of the two paired elastic clamping plates (701) are inclined inwards, and the clamping handle section (7003) is in a semicircular arc shape.
5. The refrigeration / heat mixing incubator according to claim 1, characterized in that: The panel (10) is provided with a first speed regulating knob (11) connected to the rotation control system and a second speed regulating knob (12) connected to the shaking bed control system, wherein the first speed regulating knob (11) controls the rotation speed of the first servo motor (5), and the second speed regulating knob controls the rotation speed of the second servo motor (9).
6. The refrigeration / heat mixing incubator according to claim 1, characterized in that: A heat-insulating elastic gasket is provided at the bottom bracket (803) of the tray (801) passing through the inner chamber (2).
7. The refrigeration / heat mixing incubator according to claim 1, characterized in that: Rotating shaft bearings (3) are clamped at the same position on both side walls of the middle of the inner chamber (2), and a rotating shaft (4) is installed between the two rotating shaft bearings (3).
8. The refrigeration / heat mixing incubator according to claim 1, characterized in that: The front end of the inner chamber (2) is provided with a door body (13) hinged to the box body (1), and the free end of the door body (13) is provided with a handle (14) that can be rotated and locked.
9. A refrigeration / heat mixing incubator according to claim 8, characterized in that: The surface of the door body (13) is provided with a double layer of temperature-insulating borosilicate transparent glass (15).