Paraffin type nucleic acid extraction and detection chip

Abstract

The utility model relates to the technical field of nucleic acid extraction and detection chips, and discloses a paraffin type nucleic acid extraction and detection chip which comprises a chip main body, a plurality of reagent cavities and sample cavities are arranged on the surface of the chip main body, and the reagent cavities and the sample cavities are connected with purification cavities arranged on the surface of the chip main body through pipe grooves. And sealing paraffin blocks are arranged in the multiple pipe grooves, a magnetic rotor is arranged in the purification cavity, and paraffin heating structures are arranged at the positions, corresponding to the sealing paraffin blocks, in the chip body. The liquid outlet sealing mode is changed from control valve sealing to paraffin sealing, control over the liquid outlet is achieved in a heating mode, the requirement for positioning accuracy is low, and the problems that in the prior art, when an instrument on a chip operates, the position of a control valve has errors, consequently, the liquid conveying accuracy is not high enough, and the liquid conveying precision is low are solved. And the detection efficiency is influenced.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extraction and detection chips, in particular to a paraffin type nucleic acid extraction and detection chip. Background Art

[0002] Magnetic bead method nucleic acid extraction and temperature-variable amplification detection is a commonly used microfluidic chip for PCR detection, which integrates nucleic acid extraction, amplification and detection. It has the advantages of being fast and reliable. For example, the Chinese patent number is: CN 216378192 U, a detection device for magnetic bead method nucleic acid extraction and nucleic acid amplification, the detection device includes a card box and a pipette, the card box includes an upper cover, a sealing gasket and an integrated reagent storage tube, the integrated reagent storage tube 3 is composed of several test tubes, and the openings of the several test tubes are fixed on the connector in sequence; a circular opening is set on the upper cover corresponding to the position of each test tube; the pipette is composed of a rubber sleeve, a needle, a pipette, and a filter layer; the rear end of the needle is connected to the pipette, and the filter layer is fixed to the rear end of the pipette. The utility model seals the integrated reagent storage tube through a sealing gasket, and uses the pipette needle to puncture for pipetting. During the detection process, the reagents are all in a closed state to prevent contamination, which is convenient for realizing POCT nucleic acid detection.

[0003] The commonly used PCR amplification detection chip liquid outlet sealing method currently adopts the control valve sealing method. However, because the control valve needs to be controlled by a motor and has high positioning accuracy requirements, this will cause errors in the control valve position when the instrument on the chip is running, resulting in insufficient liquid transfer accuracy and affecting detection efficiency. Utility Model Content

[0004] The utility model aims to solve the problems existing in the prior art and proposes a paraffin type nucleic acid extraction and detection chip.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A paraffin-based nucleic acid extraction and detection chip comprises a chip body, wherein a plurality of reagent chambers and sample chambers are arranged on the surface of the chip body, wherein the plurality of reagent chambers and sample chambers are connected to a purification chamber arranged on the surface of the chip body through tube grooves, and sealed paraffin blocks are arranged in the plurality of tube grooves, a magnetic rotor is arranged inside the purification chamber, a paraffin heating structure is arranged at a position inside the chip body corresponding to the sealed paraffin block, a magnet turntable is arranged at a position inside the chip body corresponding to the purification chamber, a rotary valve is arranged at the output end of the purification chamber, a waste liquid chamber and a reaction chamber are also arranged on the chip body, and the input ends of the waste liquid chamber and the reaction chamber are adapted to the rotary valve.

[0007] Preferably, the purification chamber and the waste liquid chamber are both connected with an exhaust passage.

[0008] Preferably, a pneumatic injector is also included, and several of the reagent chambers, sample chambers, and exhaust channels are provided with interfaces adapted to the pneumatic injector.

[0009] Preferably, the paraffin heating structure comprises a slide groove and a heating block, the heating block is slidably connected to the slide groove, and the slide groove passes through to the back side of the chip body.

[0010] Preferably, the reaction chamber is provided in plurality, and the output ends of the plurality of reaction chambers are connected to a liquid distributor, and the input end of the liquid distributor is adapted to a rotary valve.

[0011] Preferably, it also includes a control system, which includes a magnet turntable motor, a magnet moving motor, a syringe moving motor, an injection rod moving motor, a heating module, a third moving motor and an optical module, the magnet turntable motor is used to rotate the magnet turntable, the magnet moving motor is used to move the magnet turntable motor, the syringe moving motor is used for a pneumatic syringe, the injection rod moving motor is used to move the injection rod of the pneumatic syringe, the heating module is used to heat the reaction chamber, the third moving motor is used to move the optical module, and the optical module is used to read the reaction chamber.

[0012] Preferably, the control system is electrically connected to the rotary valve and the heating block.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] The utility model changes the sealing mode of the liquid outlet from control valve sealing to paraffin sealing, realizes the control of the liquid outlet by heating, and has relatively low requirements for positioning accuracy. It solves the problem that the prior art may cause errors in the position of the control valve when the instrument on the chip is running, thereby causing insufficient liquid transmission accuracy and affecting the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a three-dimensional schematic diagram of the paraffin-based nucleic acid extraction and detection chip proposed by the utility model;

[0016] Figure 2 This is a front schematic diagram of the paraffin-based nucleic acid extraction and detection chip proposed by the utility model;

[0017] Figure 3 This is a front section view of the paraffin-type nucleic acid extraction and detection chip proposed by the utility model;

[0018] Figure 4 This is a schematic diagram of the control system of the paraffin-based nucleic acid extraction and detection chip proposed in the present invention.

[0019] In the figure: 1. chip body; 2. reagent chamber; 3. sample chamber; 4. tube slot; 5. sealed paraffin block; 6. magnetic rotor; 7. purification chamber; 8. magnet turntable; 9. rotary valve; 10. waste liquid chamber; 11. reaction chamber; 12. exhaust channel; 13. pneumatic syringe; 14. interface; 15. slide; 16. heating block; 17. liquid dispenser; 18. magnet turntable motor; 19. magnet moving motor; 20. syringe moving motor; 21. injection rod moving motor; 22. heating module; 23. third moving motor; 24. optical module. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0021] Reference Figure 1-Figure 4 A paraffin-based nucleic acid extraction and detection chip comprises a chip body 1, wherein a plurality of reagent chambers 2 and sample chambers 3 are arranged on the surface of the chip body 1, wherein the plurality of reagent chambers 2 and sample chambers 3 are connected to a purification chamber 7 arranged on the surface of the chip body 1 through a tube groove 4, and a sealed paraffin block 5 is arranged in each of the plurality of tube grooves 4, wherein the tube groove 4 is opened on the surface of the chip body 1, a magnetic rotor 6 is arranged inside the purification chamber 7, a paraffin heating structure is arranged at a position corresponding to the sealed paraffin block 5 inside the chip body 1, a magnet turntable 8 is arranged at a position corresponding to the purification chamber 7 inside the chip body 1, a rotary valve 9 is arranged at the output end of the purification chamber 7, a waste liquid chamber 10 and a reaction chamber 11 are further arranged on the chip body 1, and the input ends of the waste liquid chamber 10 and the reaction chamber 11 are adapted to the rotary valve 9.

[0022] When using this device:

[0023] (1) First, various reagents, including lysate, eluent, washing solution, etc., are placed in each reagent chamber 2 of the chip body 1 according to the reagent requirements, and the lower outlet of each chamber is sealed with a sealing paraffin block 5;

[0024] (2) During the test, use a pipette to add the sample and magnetic beads into the sample chamber 3, then close the lid and place the chip body 1 into the detection instrument;

[0025] (3) The paraffin heating block 16 is moved to the back of the lysate outlet by operating the instrument, the sealed paraffin block 5 is heated and melted, and then the air pressure injector 13 is moved to the interface 14 at the upper end of the lysate (the rotary valve 9 is in a closed state at this time). The injector generates air pressure, and the lysate enters the purification chamber 7 through the flow channel. Then, the sample is transferred to the purification chamber 7 in the same way;

[0026] (4) After the above operation is completed, the magnet turntable 8 is rotated, and the magnet turntable 8 drives the magnetic stirrer to stir in the purification chamber 7 to allow the magnetic beads and the reagent to be fully mixed. In this step, the sample will be lysed and the released nucleic acid will be adsorbed on the magnetic beads;

[0027] (5) The magnet turntable 8 stops rotating, the magnetic beads are adsorbed onto the tube wall, the rotary valve 9 is rotated, the flow channel opening of the rotary valve 9 is moved to the position of the waste liquid chamber 10, and then the air pressure injector 13 is moved to the interface 14 at the upper end of the lysate, the injector generates air pressure, and the liquid in the purification chamber 7 is injected into the waste liquid chamber 10;

[0028] (6) Then move the rotary valve 9 to the waste liquid chamber 10, and transfer the washing liquid to the purification chamber 7 in the same way as above, then stir and mix, and then transfer the waste liquid to the waste liquid chamber 10;

[0029] (7) Continue using the same method to aspirate the eluent into the purification chamber 7 to elute the nucleic acid on the magnetic beads, then transfer the eluent to the reaction chamber 11, move the rotary valve 9 to the closed position, and then the instrument performs variable temperature cycle heating on the reaction chamber 11. After the reagents are amplified, the optical module 24 of the instrument can read whether the sample is positive.

[0030] In this embodiment, the purification chamber 7 and the waste liquid chamber 10 are both connected to an exhaust channel 12, and the exhaust channel 12 is used to exhaust gas to improve detection accuracy.

[0031] Compared with the existing technology, this device changes the sealing method of the liquid outlet from control valve sealing to paraffin sealing, and realizes the control of the liquid outlet by heating. It has relatively low requirements for positioning accuracy, and solves the problem that the existing technology will cause errors in the control valve position when the instrument on the chip is running, thereby resulting in insufficient liquid transfer accuracy and affecting detection efficiency.

[0032] In this embodiment, a pneumatic injector 13 is also included. Some of the reagent chambers 2, sample chambers 3, and exhaust channels 12 are provided with interfaces 14 adapted to the pneumatic injector 13, so as to facilitate the use of the pneumatic injector 13 to push the liquid and discharge the gas.

[0033] In this embodiment, the paraffin heating structure includes a slide groove 15 and a heating block 16. The heating block 16 is slidably connected to the slide groove 15. The slide groove 15 passes through the back of the chip body 1. Through external equipment, the heating block 16 can be controlled to move in the slide groove 15 to melt the specified sealed paraffin block 5.

[0034] In this embodiment, the reaction chamber 11 is provided in plurality, and the output ends of the plurality of reaction chambers 11 are connected to the liquid distributor 17, and the input end of the liquid distributor 17 is adapted to the rotary valve 9, so as to facilitate multiple reactions to be performed simultaneously and obtain multiple detection results.

[0035] In this embodiment, a control system is also included, which includes a magnet turntable motor 18, a magnet moving motor 19, a syringe moving motor 20, an injection rod moving motor 21, a heating module 22, a third moving motor 23 and an optical module 24. The magnet turntable motor 18 is used to rotate the magnet turntable 8, the magnet moving motor 19 is used to move the magnet turntable motor 18, the syringe moving motor 20 is used for the pneumatic syringe 13, the injection rod moving motor 21 is used to move the injection rod of the pneumatic syringe 13, the heating module 22 is used to heat the reaction chamber 11, the third moving motor 23 is used to move the optical module 24, and the optical module 24 is used to read the reaction chamber 11.

[0036] In this embodiment, the control system is electrically connected to the rotary valve 9 and the heating block 16 .

[0037] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A paraffin-based nucleic acid extraction and detection chip, comprising a chip body (1), characterized in that: The surface of the chip body (1) is provided with a plurality of reagent chambers (2) and sample chambers (3), and the plurality of reagent chambers (2) and sample chambers (3) are connected to a purification chamber (7) provided on the surface of the chip body (1) through a tube groove (4), and a sealed paraffin block (5) is provided in each of the tube grooves (4). A magnetic rotor (6) is provided inside the purification chamber (7), and a paraffin heating structure is provided at a position corresponding to the sealed paraffin block (5) inside the chip body (1). A magnetic turntable (8) is provided at a position corresponding to the purification chamber (7) inside the chip body (1), and a rotary valve (9) is provided at the output end of the purification chamber (7). A waste liquid chamber (10) and a reaction chamber (11) are also provided on the chip body (1), and the input ends of the waste liquid chamber (10) and the reaction chamber (11) are adapted to the rotary valve (9).

2. The paraffin-based nucleic acid extraction and detection chip according to claim 1, characterized in that: The purification chamber (7) and the waste liquid chamber (10) are both connected to an exhaust passage (12).

3. The paraffin-based nucleic acid extraction and detection chip according to claim 2, characterized in that: It also includes a pneumatic syringe (13), and a plurality of the reagent chambers (2), the sample chambers (3), and the exhaust channel (12) are all provided with interfaces (14) adapted to the pneumatic syringe (13).

4. The paraffin-based nucleic acid extraction and detection chip according to claim 3, characterized in that: The paraffin heating structure comprises a slide groove (15) and a heating block (16), wherein the heating block (16) is slidably connected to the slide groove (15), and the slide groove (15) passes through to the back side of the chip body (1).

5. The paraffin-based nucleic acid extraction and detection chip according to claim 4, characterized in that: The reaction chambers (11) are provided in plurality, and the output ends of the plurality of reaction chambers (11) are connected to a liquid distributor (17), and the input end of the liquid distributor (17) is adapted to the rotary valve (9).

6. The paraffin-based nucleic acid extraction and detection chip according to claim 5, characterized in that: The invention also includes a control system, which includes a magnet turntable motor (18), a magnet moving motor (19), a syringe moving motor (20), an injection rod moving motor (21), a heating module (22), a third moving motor (23) and an optical module (24). The magnet turntable motor (18) is used to rotate the magnet turntable (8), the magnet moving motor (19) is used to move the magnet turntable (8) motor, the syringe moving motor (20) is used for the pneumatic syringe (13), the injection rod moving motor (21) is used to move the injection rod of the pneumatic syringe (13), the heating module (22) is used to heat the reaction chamber (11), the third moving motor (23) is used to move the optical module (24), and the optical module (24) is used to read the reaction chamber (11).

7. The paraffin-based nucleic acid extraction and detection chip according to claim 6, characterized in that: The control system is electrically connected to the rotary valve (9) and the heating block (16).

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