Device for assisting fixation of mouse lymph node for multi-photon in-vivo imaging

Abstract

The utility model belongs to the technical field of medical image processing, and particularly relates to a device for assisting in fixing mouse lymph nodes and used for multi-photon living body imaging, which comprises a fixing ring, an observation cavity penetrating up and down is arranged in the fixing ring, and a glass sheet is assembled on the upper side of the fixing ring. The outer side of the fixing ring is provided with a negative pressure suction unit communicated with the observation cavity, the negative pressure suction unit comprises a negative pressure suction device, the negative pressure suction device is communicated with the observation cavity through a catheter, the outer side of the fixing ring is fixedly connected with an inner connector communicated with the observation cavity, and the end, away from the negative pressure suction device, of the catheter is fixedly connected with an outer connector. And the outer joint is detachably assembled on the outer side of the inner joint. According to the utility model, the lymph node tissue of a mouse can be fixed on the glass sheet by utilizing negative pressure, so that the lymph node of the mouse is fixed, and meanwhile, the damage to the lymph node and adnexal tissue is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of medical image processing, and in particular relates to a device for assisting in fixing mouse lymph nodes for multi-photon in vivo imaging. Background Art

[0002] Lymph nodes are one of the important secondary lymphoid organs for generating immune responses. High endothelial venules (HEVs) are special post-capillary venules in lymph nodes, which are the channels through which lymphocytes return from blood circulation to lymphoid organs and lymphoid tissues.

[0003] Therefore, it is very important to conduct scientific research on HEV under physiological and pathological conditions, and the technical difficulty is to perform multiphoton in vivo imaging on it;

[0004] A key step in the imaging process is to fix the lymph node tissue so that it does not move, thereby achieving better imaging results. The current method is to open a larger opening in the mouse inguinal lymph node to fully expose the lymph node, then fix the skin, use glue to build a reservoir around the lymph node, and then add saline to the reservoir before taking pictures;

[0005] Although this shooting method can fix the lymph node tissue, it is easy to cause damage to the mouse's lymph nodes and accessory tissues while fixing the lymph nodes. Utility Model Content

[0006] The utility model aims to provide a device for assisting in fixing mouse lymph nodes for multi-photon in vivo imaging, which can reduce the damage to the lymph nodes and accessory tissues while fixing the mouse lymph nodes.

[0007] The technical solution adopted by the utility model is as follows:

[0008] A device for assisting in fixing mouse lymph nodes for multi-photon in vivo imaging comprises a fixing ring, wherein an observation cavity penetrating from top to bottom is provided inside the fixing ring, a glass sheet is mounted on the upper side of the fixing ring, and a negative pressure suction unit connected to the observation cavity is mounted on the outer side of the fixing ring.

[0009] The negative pressure suction unit comprises a negative pressure aspirator, which is connected with an observation cavity through a catheter.

[0010] Furthermore, the outer side of the fixing ring is fixedly connected with an inner joint which is in communication with the observation cavity, and the end of the catheter away from the negative pressure aspirator is fixedly connected with an outer joint, and the outer joint is detachably assembled on the outer side of the inner joint.

[0011] Furthermore, the fixing ring comprises a hard ring body, the glass sheet is assembled on the upper side of the hard ring body, the inner joint is fixedly connected to the outer side of the hard ring body, and a latex ring is fixedly connected to the lower side of the hard ring body.

[0012] Furthermore, two leather pushing blocks are installed inside the hard ring body, and two moving groups capable of driving the leather pushing blocks to move are installed on the hard ring body.

[0013] Furthermore, the moving group includes a sliding rod slidably connected to the hard ring body, one end of the sliding rod located inside the hard ring body is fixedly connected to the pushing leather block, the inner wall of the hard ring body is fixedly connected with an elastic sealing sleeve, and the elastic sealing sleeve covers the outside of the combination of the rod body located inside the hard ring body and the pushing leather block.

[0014] The technical effect achieved by the utility model is as follows: the utility model is a device for auxiliary fixation of mouse lymph nodes for multi-photon in vivo imaging, which can form a negative pressure environment on the body surface of the mouse through the cooperation of a fixing ring, a glass sheet and a negative pressure suction unit, and use the negative pressure to fix the mouse lymph node tissue on the glass sheet, thereby reducing the body surface opening of the mouse when observing the mouse lymph node tissue, so that the body surface opening of the mouse is smaller and shallower, and there is no need to deeply separate the tissue, and blood vessels are almost not encountered. While fixing the mouse lymph nodes, the damage to the lymph nodes and accessory tissues is reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the structure of Example 1 of the utility model;

[0016] Figure 2 It is a schematic structural diagram of the negative pressure suction unit in Embodiment 2 of the present utility model;

[0017] Figure 3 It is a schematic diagram of the structure of Embodiment 3 of the present utility model;

[0018] Figure 4 It is a schematic diagram of the cross-section structure of the fixing ring in Embodiment 3 of the present utility model;

[0019] Figure 5 It is a schematic diagram of the cross-sectional structure of the fixing ring in Example 4 of the utility model.

[0020] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0021] 1. Fixing ring; 2. Glass slide; 3. Multiphoton microscope lens; 4. Negative pressure aspirator; 5. Catheter; 6. External joint; 7. Internal joint; 8. Hard ring body; 9. Sliding rod; 10. Pushing leather block; 11. Elastic sealing sleeve; 12. Latex ring. DETAILED DESCRIPTION

[0022] In order to make the purpose and advantages of the utility model more clear, the utility model is specifically described in combination with the following embodiments. It should be understood that the following text is only used to describe one or several specific implementations of the utility model, and does not strictly limit the protection scope of the specific request of the utility model.

[0023] Embodiment 1:

[0024] like Figure 1 As shown, a device for assisting in fixing mouse lymph nodes for multi-photon in vivo imaging comprises a fixing ring 1, which is an annular structure, and has an observation cavity which is through from top to bottom, and the upper opening and the lower opening of the observation cavity are opposite to each other. A glass sheet 2 is mounted on the upper side of the fixing ring 1, and the glass sheet 2 can be fixedly connected to the upper side of the fixing ring 1, or can be directly placed on the upper side of the fixing ring 1. A negative pressure suction unit connected to the observation cavity is mounted on the outer side of the fixing ring 1. When the lymph nodes of the mouse need to be observed, the fixing ring 1 is placed on the body surface of the mouse, and the fixing ring 1 is located outside the incision of the mouse, and the incision can be 1CM. Then, the glass sheet 2 is fixed on the upper side of the fixing ring 1, and the gas inside the observation cavity is extracted by the negative pressure suction unit, so that a negative pressure is formed inside the observation cavity. Under the action of the negative pressure, the lymph node tissue of the mouse will fit closely with the glass sheet 2, and the fixation of the lymph node tissue is completed. At this time, the multi-photon microscope lens 3 can be placed on the upper side of the glass sheet 2 to observe the lymph node tissue of the mouse.

[0025] In this embodiment, through the cooperation of the fixing ring 1, the glass sheet 2 and the negative pressure suction unit, a negative pressure environment can be formed on the body surface of the mouse, and the mouse's lymph node tissue can be fixed on the glass sheet 2 by using negative pressure, thereby reducing the opening on the mouse's body surface when observing the mouse's lymph node tissue, making the mouse's body surface opening smaller and shallower, without the need for deep separation of the tissue, and almost no blood vessels are encountered. While fixing the mouse's lymph nodes, damage to the lymph nodes and accessory tissues is reduced.

[0026] Embodiment 2:

[0027] like Figure 1-2 As shown, this embodiment discloses the structure of the negative pressure suction unit on the basis of Embodiment 1, specifically, the negative pressure suction unit includes a negative pressure suction device 4, and the negative pressure suction device 4 is connected to the observation chamber through a catheter 5. At this time, by starting the negative pressure suction device 4, the gas inside the observation chamber can be extracted, and through the setting of the catheter 5, the position of the negative pressure suction device 4 will not be restricted by the fixing ring 1, which can facilitate the user to operate the negative pressure suction device 4.

[0028] Here, the negative pressure suction device 4 can be an air pump or other equipment capable of extracting air.

[0029] Moreover, as one of the preferred embodiments of the negative pressure suction unit, the outer side of the fixing ring 1 can be fixedly connected with an inner joint 7 which is connected with the observation chamber, and the end of the catheter 5 away from the negative pressure suction device 4 can be fixedly connected with an outer joint 6, and the outer joint 6 can be detachably assembled on the outer side of the inner joint 7, and the assembly method is preferably a threaded connection.

[0030] At this time, when the fixing ring 1 is contaminated after use, only the fixing ring 1 needs to be replaced, thereby reducing the cost of use.

[0031] Embodiment 3:

[0032] See also Figure 1-4 As shown, this embodiment discloses the structure of the fixing ring 1 on the basis of the embodiment 2, the fixing ring 1 includes a hard ring body 8, the material of the hard ring body 8 is preferably plastic, the glass sheet 2 is assembled on the upper side of the hard ring body 8, the inner joint 7 is fixedly connected to the outer side of the hard ring body 8, and the lower side of the hard ring body 8 is fixedly connected with a latex ring 12. At this time, the material setting of the latex ring 12 can adapt to the body surface of the mouse and reduce the gas leakage inside the fixing ring 1.

[0033] In order to ensure the deformation performance of the latex ring 12, the latex ring 12 can be a hollow structure.

[0034] like Figure 3-4 As shown, two push leather blocks 10 are installed inside the hard ring body 8, and two moving groups that can drive the push leather blocks 10 to move are installed on the hard ring body 8. At this time, the push leather blocks 10 can be driven to move by starting the moving groups;

[0035] During observation, the fixing ring 1 is placed on the body surface of the mouse, and the lower ends of the two skin pushing blocks 10 are respectively against the skin on both sides of the mouse incision, and then the sliding rod 9 is driven to move by the moving group to pull the skin on both sides of the mouse incision away from each other, so that the lymph node tissue at the mouse incision is more fully exposed.

[0036] Here, in order to ensure that the lower end of the pushing skin block 10 can be tightly against the mouse body surface, the lower end height of the pushing skin block 10 can be set lower than the lower end height of the hard ring body 8, preferably lower than the lower end height of the latex ring 12.

[0037] Embodiment 4:

[0038] See also Figure 3-4 As shown, this embodiment discloses the structure of the moving group on the basis of Embodiment 3. The moving group includes a slide rod 9 slidably connected to the hard ring body 8. One end of the slide rod 9 located inside the hard ring body 8 is fixedly connected to the leather pushing block 10. At this time, the slide rod 9 is moved by the user's fingers, and the slide rod 9 can drive the leather pushing block 10 to move together. Its structure is relatively simple and the cost is low.

[0039] At the same time, in order to reduce air leakage, the inner wall of the hard ring body 8 is fixedly connected with an elastic sealing sleeve 11, and the elastic sealing sleeve 11 covers the outer side of the combination of the rod body of the sliding rod 9 located inside the hard ring body 8 and the push leather block 10.

[0040] The above is only a preferred embodiment of the present invention. It should be noted that, for ordinary technicians in the technical field, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention shall be implemented according to the conventional means in the field unless otherwise specified and limited.

Claims

1. A device for assisting in fixing mouse lymph nodes for multiphoton in vivo imaging, characterized in that: It comprises a fixing ring (1), wherein an observation cavity which passes through the fixing ring (1) from top to bottom is provided inside the fixing ring (1), a glass sheet (2) is provided on the upper side of the fixing ring (1), and a negative pressure suction unit which is connected to the observation cavity is provided on the outer side of the fixing ring (1); The negative pressure suction unit comprises a negative pressure suction device (4), and the negative pressure suction device (4) is connected to the observation cavity through a catheter (5); The outer side of the fixing ring (1) is fixedly connected to an inner joint (7) which is in communication with the observation chamber, and the end of the catheter (5) which is away from the negative pressure suction device (4) is fixedly connected to an outer joint (6), and the outer joint (6) is detachably mounted on the outer side of the inner joint (7).

2. The device for assisting in fixing mouse lymph nodes for multiphoton in vivo imaging according to claim 1, characterized in that: The fixing ring (1) comprises a hard ring body (8), the glass sheet (2) is mounted on the upper side of the hard ring body (8), the inner joint (7) is fixedly connected to the outer side of the hard ring body (8), and a latex ring (12) is fixedly connected to the lower side of the hard ring body (8).

3. The device for assisting in fixing mouse lymph nodes for multiphoton in vivo imaging according to claim 2, characterized in that: Two leather pushing blocks (10) are installed inside the hard ring body (8), and two moving groups capable of driving the leather pushing blocks (10) to move are installed on the hard ring body (8).

4. The device for assisting in fixing mouse lymph nodes for multiphoton in vivo imaging according to claim 3, characterized in that: The moving group comprises a sliding rod (9) slidably connected to the hard ring body (8); one end of the sliding rod (9) located inside the hard ring body (8) is fixedly connected to a push leather block (10).

5. The device for assisting in fixing mouse lymph nodes for multiphoton in vivo imaging according to claim 4, characterized in that: An elastic sealing sleeve (11) is fixedly connected to the inner wall of the hard ring body (8), and the elastic sealing sleeve (11) covers the outer side of the combination of the rod body of the sliding rod (9) located inside the hard ring body (8) and the pushing leather block (10).

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