Device for culturing cells and separating cell products

Abstract

The utility model relates to a device for culturing cells and separating cell products. Specifically, the utility model provides a device for cell culture and cell product separation, which comprises a separation column, a power cavity, a first pipeline, a second pipeline, a third pipeline, a first bottle body, a second bottle body, a third bottle body and a cell bioreactor. The device for cell culture and separation of the cell products can efficiently produce and separate the cell products such as antibodies, and can reduce the sterile detection cost of the device before cell culture, so that industrial preparation of the cell products such as the antibodies is promoted.

Description

Technical Field

[0001] The utility model relates to the field of biomedicine, and in particular to a device for cell culture and separation of cell products. Background Art

[0002] The use of cell culture technology in a cell bioreactor to produce antibodies is a commonly used method in the field of biotechnology. For example, the gene that produces the antibody is introduced into the cell, the transgenic cells are cultured in a cell bioreactor to obtain a cell culture fluid containing the antibody, and then the desired antibody is separated from the cell culture fluid containing the antibody.

[0003] At present, the cell culture fluid containing antibodies in the bioreactor is often filtered by microporous membrane filtration to separate the desired antibodies. The microporous membrane retains the cells, and the antibodies with smaller molecules are filtered into the filtrate, and then the desired antibodies are separated from the filtrate containing the antibodies. However, for the existing commonly used microporous membrane filtration device or method, when using the microporous membrane filtration, the flow direction of the cell culture fluid containing antibodies is consistent with the filtration direction of the microporous membrane. In this way, the thick filter cake layer formed by the accumulation of cells or solids on the surface of the microporous membrane will block the filtration holes, resulting in a decrease in filtration speed and filtration efficiency. The membrane needs to be cleaned or replaced frequently, thereby limiting the application of filtering and separating antibodies from cell culture fluids. In addition, microporous membrane filtration is often only suitable for filtering a small amount of cell culture fluid, which further limits the application of producing antibodies through cell culture.

[0004] In addition, for equipment used for cell culture and separation of cell product antibodies, sterility testing is required before cell culture, especially for sterility testing of filtering components such as microporous membrane filters. The filtering components are key components and are also components that are difficult to sterilize. However, for existing cell culture and separation of cell product antibody equipment, cell bioreactors and filtering components are often combined together for sterility testing. During the sterility testing process, a sterile cell culture medium accounting for about one-third of the volume of the cell bioreactor is first added to the cell bioreactor, and then the cell culture medium flows through filtering components such as microporous membrane filters. The filtrate is then collected from the filtering components and the collected filtrate is subjected to a sterility test. If the microbial test in the filtrate fails, such as the presence of bacteria, the filtering components such as the microporous filter membrane and the bioreactor need to be cleaned and sterilized again, resulting in a large amount of cell culture medium in the bioreactor Waste, thereby increasing the cost of sterility testing.

[0005] Therefore, there is a need in the art to develop a device for cell culture and separation of cell products, which can efficiently produce and separate cell products such as antibodies and can reduce the cost of sterility testing of the device before cell culture. Utility Model Content

[0006] The utility model aims to provide a device for cell culture and separation of cell products, wherein the device can efficiently produce and separate cell products such as antibodies and can reduce the cost of sterility testing of the device before cell culture.

[0007] The utility model provides a device for cell culture and separation of cell products, the device comprising a separation column, a power chamber 1, a first pipeline 2, a second pipeline 3, a third pipeline 4, a first bottle body 5, a second bottle body 6, a third bottle body 7 and a cell bioreactor 8;

[0008] The separation column comprises a first shell 9 and a filter column, the filter column comprises a hollow inner cavity 10, and the outer wall of the filter column and the first shell form an outer cavity 11;

[0009] The bottom of the hollow inner cavity of the filter column is connected to the power cavity through a pipeline. The cavity wall of the power cavity is an elastic wall. The power cavity is located in the second shell 12. The outer cavity wall of the power cavity and the inner wall of the second shell form a closed cavity 13. The second shell is connected to the air pump 14 and the first air booster pump 15 through a gas pipe.

[0010] One end of the first pipe is in communication with the bottom of the hollow inner cavity of the filtration column, and the other end of the first pipe is inserted into the bottom of the bottle cavity of the first bottle body; one end of the second pipe is in communication with the top of the outer cavity 11, and the other end of the second pipe is inserted into the bottom of the bottle cavity of the second bottle body; one end of the third pipe is in communication with the top of the hollow inner cavity of the filtration column, and the other end of the third pipe is connected to one joint of the three-way joint 16, and the other two joints of the three-way joint are respectively connected to one end of the liquid pipe 17 and the feed liquid pipe 18, the other end of the liquid pipe is inserted into the bottom of the bottle cavity of the third bottle body, and the other end of the feed liquid pipe is connected to the cell bioreactor;

[0011] The first bottle body is provided with a first sterile air filter 19, the second bottle body is provided with a second sterile air filter 20, and the third bottle body is provided with a third sterile air filter 21; the first sterile air filter, the second sterile air filter and the third sterile air filter are detachably connected to the second air booster pump 22 through gas pipes respectively;

[0012] The first pipeline, the liquid pipe and the feed liquid pipe are all provided with valves 23 .

[0013] Preferably, the bottle body comprises a cylindrical bottle body.

[0014] Preferably, the filter column is a cylindrical filter column.

[0015] Preferably, the column side wall of the filter column contains filter holes 24.

[0016] Preferably, the hollow inner cavity is a cylindrical hollow inner cavity.

[0017] Preferably, the column comprises a cylindrical column.

[0018] Preferably, the filtration column comprises a filtration membrane column.

[0019] Preferably, the filtration column comprises a hollow fiber membrane column.

[0020] Preferably, the filtration column is capable of retaining cells and the cell product antibodies are capable of passing through the filtration column.

[0021] Preferably, the outer cavity formed by the outer side wall of the filter column and the first shell is a closed cavity.

[0022] Preferably, a peristaltic pump is provided on the second pipeline.

[0023] Preferably, the power chamber comprises a spherical power chamber.

[0024] Preferably, the cavity wall of the power cavity is a silicone elastic wall or a rubber elastic wall.

[0025] Preferably, the air extraction pump comprises a vacuum pump.

[0026] Preferably, the vacuum pump comprises an air vacuum pump.

[0027] Preferably, the second shell is connected to the air pump via a separate gas pipe.

[0028] Preferably, the vacuum pump is communicated with the closed cavity 13 .

[0029] Preferably, the vacuum pump can evacuate the closed cavity 13 formed by the outer wall of the power cavity and the inner wall of the second shell.

[0030] Preferably, the cavity wall of the power cavity can expand as the air pressure of the closed cavity formed by the cavity outer wall of the power cavity and the shell inner wall of the second shell decreases.

[0031] Preferably, the second shell is connected to the first air booster pump through a separate gas pipe.

[0032] Preferably, the first air booster pump is in communication with the closed cavity 13 .

[0033] Preferably, the first air booster pump is capable of pressurizing the closed cavity 13 formed by the outer wall of the power cavity and the inner wall of the second shell.

[0034] Preferably, the cavity wall of the power cavity can shrink as the air pressure of the closed cavity formed by the cavity outer wall of the power cavity and the shell inner wall of the second shell increases.

[0035] Preferably, the closed cavity contains air.

[0036] Preferably, the bottom of the hollow inner cavity of the filter column is connected to the power cavity through a pipeline.

[0037] Preferably, a sterile filter is provided on the liquid feed pipe.

[0038] Preferably, the cell bioreactor comprises a cell bioreactor tank.

[0039] Preferably, the cells are cultured in the cell bioreactor.

[0040] Preferably, the other end of the feed liquid tube is inserted into the bottom of the tank cavity of the cell bioreactor.

[0041] Preferably, the first sterile air filter, the second sterile air filter and the third sterile air filter are capable of filtering the air to be sterile.

[0042] Preferably, the first sterile air filter is located above the first bottle body.

[0043] Preferably, the first sterile air filter is connected to the first bottle body through a gas tube.

[0044] Preferably, the first sterile air filter comprises a membrane filter.

[0045] Preferably, the second sterile air filter is located above the second bottle body.

[0046] Preferably, the second sterile air filter is connected to the second bottle body through a gas pipe.

[0047] Preferably, the second sterile air filter comprises a membrane filter.

[0048] Preferably, the third sterile air filter is located above the third bottle body.

[0049] Preferably, the third sterile air filter is connected to the third bottle body through a gas pipe.

[0050] Preferably, the third sterile air filter comprises a membrane filter.

[0051] Preferably, the first sterile air filter is detachably connected to a separate second air booster pump via a separate gas tube.

[0052] Preferably, the second sterile air filter is detachably connected to a separate second air booster pump via a separate gas tube.

[0053] Preferably, the third sterile air filter is detachably connected to a separate second air booster pump via a separate gas tube.

[0054] Preferably, the membrane comprises a filtration membrane.

[0055] Preferably, the pore size of the filter membrane is 0.22 μm.

[0056] Preferably, a liquid outlet pipe is provided at the bottom of the first bottle body, the second bottle body and / or the third bottle body.

[0057] Preferably, the liquid outlet pipe is provided with a valve.

[0058] Preferably, the gas pipe is provided with a valve.

[0059] Preferably, the second conduit is provided with a valve.

[0060] Preferably, the third pipeline is provided with a valve.

[0061] Preferably, the pipeline comprises a liquid conduit.

[0062] Preferably, the connection or connection includes communication.

[0063] Preferably, the connection is a fixed connection or a detachable connection.

[0064] Preferably, the device further comprises a cell culture medium liquid feeder, and the cell culture medium liquid feeder is connected to the cell bioreactor via a liquid tube.

[0065] Preferably, the cell culture medium feeder contains sterile cell culture medium

[0066] Preferably, the cell culture medium feeder is capable of adding sterile cell culture medium to the cell bioreactor.

[0067] Preferably, the cell culture medium feeder is capable of adding sterile cell culture medium into the cell bioreactor.

[0068] Preferably, the cell product comprises an antibody.

[0069] It should be understood that within the scope of the present invention, the above-mentioned technical features of the present invention and the technical features specifically described below (such as embodiments) can be combined with each other to form new or preferred technical solutions. BRIEF DESCRIPTION OF THE DRAWINGS

[0070] It should be understood that the drawings are only used for illustrative purposes and should not be construed as limitations on this patent; in order to better illustrate this embodiment, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of the actual product; for those skilled in the art, it is understandable that some well-known structures and their descriptions in the drawings may be omitted; the same or similar numbers correspond to the same or similar parts; the terms describing the positional relationship in the drawings are only used for illustrative purposes and should not be construed as limitations on this patent.

[0071] Figure 1 Schematic diagram of the structure of a device for cell culture and separation of cell products.

[0072] Wherein, each number in the accompanying drawings represents:

[0073] 1 is a power chamber, 2 is a first pipeline, 3 is a second pipeline, 4 is a third pipeline, 5 is a first bottle body, 6 is a second bottle body, 7 is a third bottle body, 8 is a cell bioreactor, 9 is a first shell, 10 is a hollow inner cavity, 11 is an outer cavity, 12 is a second shell, 13 is a closed cavity, 14 is an air pump, 15 is a first air booster pump, 16 is a three-way joint, 17 is a liquid pipe, 18 is a liquid pipe, 19 is a first sterile air filter, 20 is a second sterile air filter, 21 is a third sterile air filter, 22 is a second air booster pump, 23 is a valve and 24 is a filter hole. DETAILED DESCRIPTION

[0074] The present inventors have developed a device for cell culture and separation of cell products after extensive and intensive research.

[0075] the term

[0076] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0077] As used herein, the terms "include", "comprises", and "comprising" are used interchangeably, and include not only open definitions, but also semi-closed and closed definitions. In other words, the terms include "consisting of", "consisting essentially of".

[0078] As used herein, the terms "upper", "lower", "bottom", "top", "side", etc., indicating orientations or positional relationships are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0079] As used herein, the terms "first", "second", and "third", etc. are used for descriptive purposes only and are not to be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, the term "first", "second", and "third", etc. may explicitly or implicitly include one or more of the features.

[0080] As used herein, the terms “connected” and “connected” should be understood in a broad sense, and may refer to fixed connection or connection, detachable connection or connection, or integral connection or connection.

[0081] For the sake of convenience, the following Figure 1 To further describe the present invention, it should be understood that the accompanying drawings do not limit the scope of the present invention.

[0082] The utility model provides a device for cell culture and separation of cell products, the device comprising a separation column, a power chamber 1, a first pipeline 2, a second pipeline 3, a third pipeline 4, a first bottle body 5, a second bottle body 6, a third bottle body 7 and a cell bioreactor 8;

[0083] The separation column comprises a first shell 9 and a filter column, the filter column comprises a hollow inner cavity 10, and the outer wall of the filter column and the first shell form an outer cavity 11;

[0084] The bottom of the hollow inner cavity of the filter column is connected to the power cavity through a pipeline. The cavity wall of the power cavity is an elastic wall. The power cavity is located in the second shell 12. The outer cavity wall of the power cavity and the inner wall of the second shell form a closed cavity 13. The second shell is connected to the air pump 14 and the first air booster pump 15 through a gas pipe.

[0085] One end of the first pipe is in communication with the bottom of the hollow inner cavity of the filtration column, and the other end of the first pipe is inserted into the bottom of the bottle cavity of the first bottle body; one end of the second pipe is in communication with the top of the outer cavity 11, and the other end of the second pipe is inserted into the bottom of the bottle cavity of the second bottle body; one end of the third pipe is in communication with the top of the hollow inner cavity of the filtration column, and the other end of the third pipe is connected to one joint of the three-way joint 16, and the other two joints of the three-way joint are respectively connected to one end of the liquid pipe 17 and the feed liquid pipe 18, the other end of the liquid pipe is inserted into the bottom of the bottle cavity of the third bottle body, and the other end of the feed liquid pipe is connected to the cell bioreactor;

[0086] The first bottle body is provided with a first sterile air filter 19, the second bottle body is provided with a second sterile air filter 20, and the third bottle body is provided with a third sterile air filter 21; the first sterile air filter, the second sterile air filter and the third sterile air filter are detachably connected to the second air booster pump 22 through gas pipes respectively;

[0087] The first pipeline, the liquid pipe and the feed liquid pipe are all provided with valves 23 .

[0088] The device for cell culture and separation of cell products described in the utility model can efficiently produce and separate cell product antibodies, and the typical working principle is as follows:

[0089] Close the valves on the liquid pipe and the first channel, open the valve on the feed liquid pipe, and the vacuum pump evacuates and depressurizes the closed cavity formed by the outer wall of the power cavity and the inner wall of the second shell, so that the cavity wall of the power cavity expands. The expansion of the cavity wall of the power cavity causes the cell culture fluid containing secreted antibodies in the cell bioreactor to enter the hollow inner cavity of the filter column and the power cavity through the feed liquid pipe and the third channel. Then, the first air booster pump inflates and pressurizes the closed cavity formed by the outer wall of the power cavity and the inner wall of the second shell, so that the cavity wall of the power cavity contracts. The contraction of the cavity wall of the power cavity causes the cell culture fluid containing secreted antibodies in the hollow inner cavity of the power cavity and the filter column to flow back into the cell bioreactor through the third channel and the feed liquid pipe. Therefore, the vacuum pump and the first air booster pump alternately evacuate and depressurize the closed cavity formed by the outer wall of the power cavity and the inner wall of the second shell. The gas is pressurized so that the cell culture fluid containing the secreted antibodies continuously flows up and down alternately in the hollow inner cavity of the filter column. During the process of the cell culture fluid containing the secreted antibodies alternatingly flowing up and down in the hollow inner cavity of the filter column, the cell product antibodies are filtered through the column side wall of the filter column into the outer cavity formed by the column outer wall of the filter column and the first shell, and then the cell product antibodies enter the second bottle body through the second pipe for collection. Since the liquid containing the cell product antibodies continuously enters the second bottle body, the amount of the cell culture fluid containing the secreted antibodies continuously decreases. Therefore, by continuously adding cell culture medium to the cell bioreactor, a balance can be achieved between continuous cell culture secreted product antibodies and secreted product antibodies continuously entering the second bottle body through the column side wall of the filter column for collection, thereby realizing continuous production and separation and collection of cell secreted product antibodies, and improving the preparation efficiency of cell secreted product antibodies. In addition, since the direction of the cell culture fluid containing secreted antibodies flowing up and down alternately in the hollow inner cavity of the filter column is perpendicular to the direction in which the cell product antibodies are filtered through the column side wall of the filter column to the outer cavity formed by the column outer wall of the filter column and the first shell, the clogging risk of the filter column can be reduced, and the efficiency of filtering and separating to obtain the cell product antibodies is improved. Therefore, the device for cell culture and separation of cell products of the utility model can efficiently produce and separate to obtain cell product antibodies.

[0090] The device for cell culture and separation of cell products described in the utility model can also reduce the cost of sterility testing of the device before cell culture. The typical sterility testing principle is as follows:

[0091] The valve of the liquid feed pipe is closed, and sterile cell culture medium is added to the first bottle body. The second air booster pump pressurizes the first bottle body through the first sterile air filter, and the sterile cell culture medium in the first bottle body enters the hollow inner cavity and the power cavity of the filter column. Then, the cell culture medium in the hollow inner cavity of the filter column enters the third bottle body through the third pipeline and the liquid pipe, and is filtered through the column side wall of the filter column to the outer cavity formed by the column outer wall of the filter column and the first shell, and then enters the second bottle body through the second pipeline. Then, the second air booster pump passes through the second sterile air filter and the third sterile air filter. The filter pressurizes the second bottle and the third bottle, and the cell culture medium that flows into the second bottle and the third bottle flows back into the first bottle. At this time, the cell culture medium that flows back into the first bottle has cleaned the separation column and the power chamber and other components. Therefore, the sterility test of the cell culture medium that flows back into the first bottle can determine whether the device meets the sterility requirements. Therefore, the device described in the utility model can achieve the sterility test of the device only by using the amount of sterile cell culture medium added to the first bottle, which greatly reduces the amount of sterile cell culture medium used for sterility testing, thereby reducing the cost of sterility testing. Of course, if the microbial test in the cell culture medium that flows back into the first bottle fails, such as the presence of bacteria, the device needs to be sterilized again and then sterility tested again using the same method as above.

[0092] In a preferred embodiment of the present invention, the column sidewall of the filtration column comprises filtration holes, which are large enough to trap cells and cell products such as antibodies can pass through the filtration holes, thereby achieving filtration separation of cell products such as antibodies.

[0093] The filter column of the present invention is not particularly limited and may be a filter membrane column such as a hollow fiber membrane column and the like.

[0094] The second pipeline of the utility model can also be provided with a peristaltic pump. The peristaltic pump can further promote the cell products such as antibodies to pass through the column side wall of the filter column to the outer cavity formed by the column outer side wall of the filter column and the first shell through peristalsis, and promote the cell products such as antibodies to enter the second bottle through the second pipeline for collection, thereby improving the filtration and separation efficiency of the cell products and antibodies.

[0095] The vacuum pump described in the utility model can evacuate the closed cavity formed by the outer wall of the power cavity and the inner wall of the second shell, and the first air booster pump can pressurize the closed cavity formed by the outer wall of the power cavity and the inner wall of the second shell. The cavity wall of the power cavity can expand as the air pressure in the closed cavity decreases and contract as the air pressure in the closed cavity increases. The vacuum pump and the first air booster pump are used to alternately evacuate and decompress the closed cavity formed by the outer wall of the power cavity and the inner wall of the second shell, and inflate and pressurize it, so that the cell culture fluid containing secreted antibodies continuously flows up and down alternately in the hollow inner cavity of the filter column.

[0096] The shape of the power chamber of the present invention is not particularly limited. Preferably, the shape of the power chamber is spherical.

[0097] The material of the power chamber of the utility model is preferably silicone or rubber, which has good expansion and contraction elasticity.

[0098] The main excellent technical effects of the utility model include:

[0099] The utility model develops a device for cell culture and separation of cell products, which can efficiently produce and separate cell products such as antibodies and can reduce the cost of sterility testing of the device before cell culture, thereby promoting industrial preparation of cell products such as antibodies.

[0100] The present invention is further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0101] Example 1

[0102] This embodiment 1 provides a device for cell culture and separation of cell products, the device comprising a separation column, a power chamber 1, a first pipeline 2, a second pipeline 3, a third pipeline 4, a first bottle body 5, a second bottle body 6, a third bottle body 7 and a cell bioreactor 8;

[0103] The separation column comprises a first shell 9 and a filter column, the filter column comprises a hollow inner cavity 10, and the outer wall of the filter column and the first shell form an outer cavity 11;

[0104] The bottom of the hollow inner cavity of the filter column is connected to the power cavity through a pipeline. The cavity wall of the power cavity is an elastic wall. The power cavity is located in the second shell 12. The outer cavity wall of the power cavity and the inner wall of the second shell form a closed cavity 13. The second shell is connected to the air pump 14 and the first air booster pump 15 through a gas pipe.

[0105] One end of the first pipe is in communication with the bottom of the hollow inner cavity of the filtration column, and the other end of the first pipe is inserted into the bottom of the bottle cavity of the first bottle body; one end of the second pipe is in communication with the top of the outer cavity 11, and the other end of the second pipe is inserted into the bottom of the bottle cavity of the second bottle body; one end of the third pipe is in communication with the top of the hollow inner cavity of the filtration column, and the other end of the third pipe is connected to one joint of the three-way joint 16, and the other two joints of the three-way joint are respectively connected to one end of the liquid pipe 17 and the feed liquid pipe 18, the other end of the liquid pipe is inserted into the bottom of the bottle cavity of the third bottle body, and the other end of the feed liquid pipe is connected to the cell bioreactor;

[0106] The first bottle body is provided with a first sterile air filter 19, the second bottle body is provided with a second sterile air filter 20, and the third bottle body is provided with a third sterile air filter 21; the first sterile air filter, the second sterile air filter and the third sterile air filter are detachably connected to the second air booster pump 22 through gas pipes respectively;

[0107] The first pipeline, the liquid pipe and the feed liquid pipe are all provided with valves 23 .

[0108] The device for cell culture and separation of cell products described in Example 1 can efficiently produce and separate cell products such as antibodies, and can reduce the cost of sterility testing of the device before cell culture.

[0109] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A device for cell culture and separation of cell products, characterized in that: The device comprises a separation column, a power chamber (1), a first pipeline (2), a second pipeline (3), a third pipeline (4), a first bottle body (5), a second bottle body (6), a third bottle body (7) and a cell bioreactor (8); The separation column comprises a first shell (9) and a filter column, the filter column comprises a hollow inner cavity (10), and the outer wall of the filter column and the first shell form an outer cavity (11); The bottom of the hollow inner cavity of the filter column is connected to the power cavity through a pipeline, the cavity wall of the power cavity is an elastic wall, the power cavity is located in a second shell (12), the outer cavity wall of the power cavity and the inner wall of the second shell form a closed cavity (13), and the second shell is connected to the air pump (14) and the first air booster pump (15) respectively through a gas pipe; One end of the first pipe is in communication with the bottom of the hollow inner cavity of the filtration column, and the other end of the first pipe is inserted into the bottom of the bottle cavity of the first bottle body; one end of the second pipe is in communication with the top of the outer cavity (11), and the other end of the second pipe is inserted into the bottom of the bottle cavity of the second bottle body; one end of the third pipe is in communication with the top of the hollow inner cavity of the filtration column, and the other end of the third pipe is connected to one joint of the three-way joint (16), and the other two joints of the three-way joint are respectively connected to one end of the liquid pipe (17) and the feed liquid pipe (18), the other end of the liquid pipe is inserted into the bottom of the bottle cavity of the third bottle body, and the other end of the feed liquid pipe is connected to the cell bioreactor; The first bottle body is provided with a first sterile air filter (19), the second bottle body is provided with a second sterile air filter (20), and the third bottle body is provided with a third sterile air filter (21); the first sterile air filter, the second sterile air filter and the third sterile air filter are detachably connected to a second air booster pump (22) via gas pipes respectively; The first pipeline, the liquid pipe and the feed liquid pipe are all provided with valves (23).

2. The device for cell culture and separation of cell products according to claim 1, characterized in that: The filter column is a cylindrical filter column.

3. The device for cell culture and separation of cell products according to claim 1, characterized in that: The column side wall of the filter column contains filter holes (24).

4. The device for cell culture and separation of cell products according to claim 1, characterized in that: The hollow inner cavity is a cylindrical hollow inner cavity.

5. The device for cell culture and separation of cell products according to claim 1, characterized in that: The filtration column comprises a filtration membrane column.

6. The device for cell culture and separation of cell products according to claim 1, characterized in that: The filtration column comprises a hollow fiber membrane column.

7. The device for cell culture and separation of cell products according to claim 1, characterized in that: The second pipeline is provided with a peristaltic pump.

8. The device for cell culture and separation of cell products according to claim 1, characterized in that: The power chamber comprises a spherical power chamber.

9. The device for cell culture and separation of cell products according to claim 1, characterized in that: The cavity wall of the power cavity is a silicone elastic wall or a rubber elastic wall.

10. The device for cell culture and separation of cell products according to claim 1, characterized in that: The cavity wall of the power cavity can expand as the air pressure of the closed cavity formed by the cavity outer wall of the power cavity and the shell inner wall of the second shell decreases; The cavity wall of the power cavity can shrink as the air pressure of the closed cavity formed by the cavity outer wall of the power cavity and the shell inner wall of the second shell increases.

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