Cell culture isolator with transfer sterilization function

By setting up a shunt tube and atomizing nozzle in the transfer chamber of the cell culture isolator and equipped with an adjustment mechanism, the problem of uneven sterilization effect in the prior art is solved, and uniform sterilization treatment of items in the transfer chamber is achieved, which significantly improves the sterilization effect.

CN119913021AInactive Publication Date: 2025-05-02SHENZHEN SVESAI BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202510105939.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-22
Publication Date
2025-05-02
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The sterilization effect of existing cell culture isolators is uneven, which causes the items in the delivery bin to be unable to be uniformly sterilized, reducing the sterilization effect.

Method used

A cell culture isolator with transfer sterilization function is designed, including a shunt tube and an atomizing spray head in the transfer chamber, and a adjustment mechanism is set on the isolator body and the shunt tube, which can adjust the use angle of the atomizing spray head and ensure that the hydrogen peroxide solution is evenly sprayed into the transfer chamber.

Benefits of technology

By adjusting the spraying angle of the atomization spray head, the hydrogen peroxide solution can be evenly sprayed into the transfer chamber, which significantly improves the sterilization effect of the item.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention is suitable for the technical field of cell culture, and provides a cell culture isolator with a transfer sterilization function, the cell culture isolator comprises an isolator body used for cell culture, the isolator body comprises a transfer bin used for transferring articles used for cell culture; the placing rack is arranged in the transfer bin and is used for placing articles; the shunting pipe is arranged in the transfer bin, and an atomizing nozzle for atomizing a hydrogen peroxide solution into the transfer bin to disinfect articles is arranged on the shunting pipe; and the adjusting mechanism is arranged on the isolator body and the shunt pipe and is used for adjusting the use angle of the atomizing nozzle. The cell culture isolator with the transfer sterilization function, provided by the scheme, can be used for culturing cells, and can be used for uniformly sterilizing used articles, so that the sterilization effect is effectively improved.
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Description

Technical Field

[0001] The invention belongs to the technical field of cell culture, and in particular relates to a cell culture isolator with a transfer sterilization function. Background Art

[0002] In the process of cell culture, the corresponding cell culture isolator is needed. The cell culture isolator is an experimental equipment that integrates a variety of cell culture-related functional components. It is mainly used to complete cell culture-related operations in a closed sterile environment to meet the production requirements of cell products GMP (Good Manufacturing Practice).

[0003] When using a cell culture isolator to culture cells, the items need to be placed in a transfer bin of the isolator first. An atomizing nozzle is provided in the transfer bin, and a hydrogen peroxide solution is atomized and sprayed into the transfer bin through the atomizing nozzle, so that the items in the transfer bin can be sterilized. However, the atomizing nozzles provided in some existing isolator transfer bins have a relatively fixed use angle, which makes it impossible to evenly spray the atomized hydrogen peroxide into the transfer bin, so that the items in the transfer bin cannot be evenly sterilized, thereby reducing the sterilization effect. Summary of the invention

[0004] The present invention provides a cell culture isolator with a transfer sterilization function, aiming to solve the problem of poor sterilization effect of the currently used cell culture isolators proposed in the above background technology.

[0005] To solve the above problems, the present invention is implemented as follows: a cell culture isolator with a transfer sterilization function, comprising: an isolator body for cell culture, the isolator body comprising a transfer bin for transferring articles used for cell culture; a placement rack arranged in the transfer bin for placing articles; a shunt pipe arranged in the transfer bin, the shunt pipe being provided with an atomizing nozzle for atomizing hydrogen peroxide solution into the transfer bin to disinfect the articles; and an adjustment mechanism arranged on the isolator body and the shunt pipe for adjusting the use angle of the atomizing nozzle.

[0006] Preferably, the adjustment mechanism includes: an installation cavity opened on the isolator body, in which a dual-axis motor is fixedly installed; two installation shafts rotatably installed in the installation cavity, and the two installation shafts are respectively fixedly connected to the two output shafts of the dual-axis motor; multiple connecting shafts rotatably installed on the isolator body, and the multiple connecting shafts are respectively fixedly connected to the two ends of the two shunt pipes; four first bevel gears fixedly mounted on the two connecting shafts and the two installation shafts, and the two first bevel gears fixedly mounted on the two connecting shafts are respectively meshed with the two first bevel gears fixedly mounted on the two installation shafts.

[0007] Preferably, the isolator body is provided with a feeding mechanism for adding the hydrogen peroxide solution into the shunt pipe, the feeding mechanism comprising: a storage cavity for placing the hydrogen peroxide solution opened on the isolator body, and a feeding port is provided on the isolator body, the feeding port is communicated with the storage cavity, and the feeding port is used to add the hydrogen peroxide solution into the storage cavity; a water pump fixedly mounted on the isolator body; a feeding pipe fixedly mounted on the isolator body, the feeding pipe is communicated with the water inlet end of the water pump, and the feeding pipe extends into the storage cavity; a connecting pipe fixedly mounted on the inner wall of one side of the transfer bin, the connecting pipe is communicated with the water outlet end of the water pump; two hoses arranged on the connecting pipe, and the two hoses are respectively connected with the two shunt pipes.

[0008] Preferably, a feeding mechanism is provided in the transfer bin for driving the items on the placement rack to move upward for feeding, and the feeding mechanism comprises: a telescopic rod fixedly mounted on the isolator body; a support seat fixedly mounted on the output shaft of the telescopic rod, the support seat is connected to the placement rack, and the support seat is used to support and place the placement rack.

[0009] Preferably, the support base is provided with a rotating mechanism for driving the items placed on the placement rack to rotate, and the rotating mechanism includes: a mounting groove provided on the support base, in which a driving motor is fixedly installed; a rotating shaft rotatably installed on the support base, the bottom end of the rotating shaft is fixedly connected to the output shaft of the driving motor, and the other end of the rotating shaft is connected to the bottom of the placement rack.

[0010] Preferably, a connecting mechanism is provided on the rotating shaft and the placing rack, and the connecting mechanism is used to connect and fix the rotating shaft and the placing rack, and the connecting mechanism includes: a connecting seat fixedly installed on the top of the rotating shaft; a connecting groove opened on the connecting seat; a connecting block fixedly installed on the bottom of the placing rack, and the connecting block and the connecting groove are adapted to be connected; and a fixing mechanism assembled on the connecting seat for fixing the connecting block in the connecting groove.

[0011] Preferably, the fixing mechanism includes: a mounting bracket fixedly mounted on one side of the connecting seat, and two placement cavities are provided on the mounting bracket; two positioning rods slidably mounted on the mounting bracket and the connecting seat; two positioning grooves provided on the connecting block, and the two positioning grooves are respectively adapted to be connected with the ends of the two positioning rods; two stoppers respectively fixedly mounted on the two positioning rods, and the two stoppers are respectively slidably connected with the inner walls of the two placement cavities; two springs respectively mounted on the two positioning rods; and an operating bracket fixedly mounted on the two positioning rods for pulling the two positioning rods.

[0012] Preferably, a plurality of sliding blocks are fixedly mounted on the bottom of the placement rack, a sliding groove is provided on the top of the support seat, the sliding groove is arranged in an annular shape, and the plurality of sliding blocks are slidably connected to the sliding groove.

[0013] Preferably, a stirring mechanism for stirring the hydrogen peroxide solution placed in the storage chamber is provided in the storage chamber, and the stirring mechanism comprises: two placement shafts rotatably installed in the storage chamber, and a plurality of stirring racks for stirring the hydrogen peroxide solution placed in the storage chamber are fixedly installed on the two placement shafts; two second bevel gears respectively fixedly sleeved on the installation shaft and one of the placement shafts, and the two second bevel gears are meshed with each other; two synchronous wheels respectively fixedly sleeved on the two placement shafts, and the two synchronous wheels are sleeved with synchronous belts.

[0014] Preferably, a hydrogen peroxide concentration sensor is provided in the transfer chamber, and the hydrogen peroxide concentration sensor is used to monitor the hydrogen peroxide concentration in the transfer chamber.

[0015] Compared with the related art, the cell culture isolator with transfer sterilization function provided by the present invention has the following beneficial effects:

[0016] Compared with the prior art, the cell culture isolator with transfer sterilization function provided by the present solution can culture cells and can evenly sterilize the items used, thereby effectively improving the sterilization effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the main structure of a cell culture isolator with transfer sterilization function provided by the present invention;

[0018] Figure 2 It is a schematic diagram of the main cross-sectional structure of the present invention;

[0019] Figure 3 for Figure 2 An enlarged structural diagram of part A shown in FIG.

[0020] Figure 4 for Figure 2 An enlarged schematic diagram of the structure of part B shown in FIG.

[0021] Figure 5 for Figure 2 An enlarged schematic diagram of the structure of part C shown in FIG.

[0022] Figure 6 for Figure 2 An enlarged structural diagram of part D shown in FIG.

[0023] Figure 7 for Figure 6 An enlarged structural diagram of part E shown in FIG.

[0024] Figure 8 for Figure 2 An enlarged structural diagram of part F shown in FIG.

[0025] Fig. 9 It is a schematic diagram of the top view of the support seat and the slide groove in the present invention;

[0026] Fig.10 It is a schematic diagram of the three-dimensional structure of the connecting seat and the connecting groove in the present invention.

[0027] Figure numerals: 1, isolator body; 101, operating room; 103, sleeve operating port; 2, transfer chamber; 201, protective door; 3, placement rack; 4, shunt pipe; 5, atomizing nozzle; 6, installation cavity; 7, dual-axis motor; 8, installation shaft; 9, connecting shaft; 901, first bevel gear; 10, storage cavity; 11, water pump; 12, feed pipe; 13, connecting pipe; 14, hose; 15, telescopic rod; 16, support seat; 17 , mounting groove; 18, driving motor; 19, rotating shaft; 20, connecting seat; 21, connecting groove; 22, connecting block; 23, mounting frame; 24, placement cavity; 25, positioning rod; 26, positioning groove; 27, stopper; 28, spring; 29, operating frame; 30, slider; 31, slide groove; 32, placement shaft; 33, stirring frame; 34, second bevel gear; 35, synchronous wheel; 36, synchronous belt; 37, hydrogen peroxide concentration sensor. DETAILED DESCRIPTION

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by technicians in the technical field of the present application; the terms used in the specification of the application herein are only for the purpose of describing specific embodiments and are not intended to limit the present application; the terms "including" and "having" in the specification and claims of the present application and the above-mentioned figure descriptions and any variations thereof are intended to cover non-exclusive inclusions. The terms "first", "second", etc. in the specification and claims of the present application or the above-mentioned figures are used to distinguish different objects, rather than to describe a specific order; the terms "inside", "outside", "left", and "right" indicate directions or positional relationships based on the directions or positional relationships shown in the drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention.

[0029] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0030] The embodiment of the present invention provides a cell culture isolator with a transfer sterilization function, such as Figure 1-10 As shown, the cell culture isolator with transfer sterilization function includes: an isolator body 1 for cell culture, the isolator body 1 includes a transfer bin 2 for transferring articles used for cell culture; a placement rack 3 arranged in the transfer bin 2 for placing articles; a shunt pipe 4 arranged in the transfer bin 2, the shunt pipe 4 is provided with an atomizing nozzle 5 for atomizing hydrogen peroxide solution into the transfer bin 2 to disinfect the articles; and an adjustment mechanism arranged on the isolator body 1 and the shunt pipe 4 for adjusting the use angle of the atomizing nozzle 5.

[0031] In this embodiment, the isolator body 1 also includes an operating room 101, the operating room 101 is provided with a sealed door, the sealed door is provided with a sleeve operating port 103 for operating in the operating room 101, and the transfer chamber 2 is provided with a protective door 201 for sealing the transfer chamber 2. When the articles used for cell culture are transferred, the protective door 201 on the transfer chamber 2 is opened, the articles are placed on the placement rack 3, and then the protective door 201 is closed to perform a disinfection operation on the articles placed on the placement rack 3. At the same time, the device A controller is provided on one side of the shunt pipe 4. The working principle of the controller is the prior art and will not be described in detail here. When sterilizing items, the feeding mechanism is started by the controller to feed the hydrogen peroxide solution into the shunt pipe 4, and then the hydrogen peroxide solution is atomized into the transfer bin 2 through the atomizing nozzle 5 to sterilize the items placed on the placement rack 3. During sterilization, the adjustment mechanism is started by the controller at the same time to drive the shunt pipe 4 to rotate at a certain angle, so that the spraying angle of the atomizing nozzle 5 on the shunt pipe 4 can be adjusted. The continuously swinging atomizing nozzle 5 can The hydrogen peroxide solution is evenly sprayed into the transfer bin 2, so that the hydrogen peroxide sterilization solution is evenly distributed in the transfer bin 2, so that the hydrogen peroxide solution can be evenly sprayed on the items placed on the placement rack 3, thereby effectively improving the sterilization effect on the items. After the sterilization is completed, the sterilized items are transferred to the operating room 101. The isolator body 1 is equipped with a high-efficiency air filter (such as a HEPA filter) to filter out tiny particles and microorganisms in the air to ensure that the air entering the isolator is sterile. The items are sterilized when entering and leaving the isolator body 1 to ensure that no contaminants are introduced during the transfer process. Then the staff operates through the rubber gloves installed on the sleeve operation port 103 to avoid direct contact with the items in the isolator body 1, further reducing the risk of contamination, thereby providing a safe culture environment for cell culture work. The working principle of the isolator body 1 is the existing technology, which will not be elaborated here. Through the entire device, cells can be cultured, and the items used can be evenly sterilized to effectively improve the sterilization effect.

[0032] In a further preferred embodiment of the present invention, the adjusting mechanism includes: an installation cavity 6 opened on the isolator body 1, a dual-axis motor 7 is fixedly installed in the installation cavity 6; two installation shafts 8 rotatably installed in the installation cavity 6, the two installation shafts 8 are respectively fixedly connected to the two output shafts of the dual-axis motor 7; a plurality of connecting shafts 9 rotatably installed on the isolator body 1, the plurality of connecting shafts 9 are respectively fixedly connected to the two ends of the two shunt pipes 4; four first bevel gears 901 are respectively fixedly mounted on the two connecting shafts 9 and the two installation shafts 8, and the two first bevel gears 901 fixedly mounted on the two connecting shafts 9 are respectively meshed with the two first bevel gears 901 fixedly mounted on the two installation shafts 8.

[0033] In this embodiment, when the atomizing nozzle 5 atomizes the hydrogen peroxide solution into the transfer bin 2 and sterilizes the items placed on the placement rack 3, the dual-axis motor 7 is started by the controller to drive the two mounting shafts 8 to rotate forward and reversely to a certain extent. Under the interaction of the four first bevel gears 901, the connecting shaft 9 can be driven to rotate, and then the two shunt pipes 4 can be driven to rotate forward and reverse at a certain angle. The rotation angle is set according to actual needs, and the operation of the dual-axis motor 7 can be set. This is the prior art and will not be elaborated here. The continuously swinging atomizing nozzle 5 can evenly spray the hydrogen peroxide solution into the transfer bin 2, so that the hydrogen peroxide sterilization solution is evenly distributed in the transfer bin 2, so that the hydrogen peroxide solution can be evenly sprayed on the items placed on the placement rack 3, thereby effectively improving the sterilization effect on the items.

[0034] In a further preferred embodiment of the present invention, the isolator body 1 is provided with a feeding mechanism for adding the hydrogen peroxide solution into the shunt pipe 4, and the feeding mechanism includes: a storage chamber 10 for placing the hydrogen peroxide solution opened on the isolator body 1, and a feeding port is provided on the isolator body 1, the feeding port is connected to the storage chamber 10, and the feeding port is used to add the hydrogen peroxide solution into the storage chamber 10; a water pump 11 fixedly mounted on the isolator body 1; a feeding pipe 12 fixedly mounted on the isolator body 1, the feeding pipe 12 is connected to the water inlet end of the water pump 11, and the feeding pipe 12 extends into the storage chamber 10; a connecting pipe 13 fixedly mounted on the inner wall of one side of the transfer bin 2, the connecting pipe 13 is connected to the water outlet end of the water pump 11; two hoses 14 arranged on the connecting pipe 13, and the two hoses 14 are respectively connected to the two shunt pipes 4.

[0035] In this embodiment, after placing the items on the placement rack 3, the protective door 201 is closed, and when the items placed on the placement rack 3 are disinfected, the water pump 11 is started by the controller, and the hydrogen peroxide solution in the storage chamber 10 enters the connecting pipe 13 through the feeding pipe 12, and then enters the two branch pipes 4 respectively through the two hoses 14, and then is atomized into the transfer bin 2 by the atomizing nozzle 5 on the branch pipe 4 to sterilize the items on the placement rack 3. When the atomizing nozzle 5 atomizes the hydrogen peroxide solution into the transfer bin 2, the spraying angle of the atomizing nozzle 5 on the branch pipe 4 can be adjusted under the action of the adjusting mechanism, and the continuously swinging atomizing nozzle 5 can evenly spray the hydrogen peroxide solution into the transfer bin 2, so that the hydrogen peroxide sterilizing solution is evenly distributed in the transfer bin 2, so that the hydrogen peroxide solution can be evenly sprayed on the items placed on the placement rack 3, thereby effectively improving the sterilization effect on the items.

[0036] In a further preferred embodiment of the present invention, a feeding mechanism is provided in the transfer bin 2 for driving the items on the placement rack 3 to move upward for feeding, and the feeding mechanism includes: a telescopic rod 15 fixedly mounted on the isolator body 1; a support seat 16 fixedly mounted on the output shaft of the telescopic rod 15, the support seat 16 is connected to the placement rack 3, and the support seat 16 is used to support and place the placement rack 3.

[0037] In this embodiment, after the items on the placement rack 3 are sterilized, when the sterilized items need to be sent to the operating room 101, the telescopic rod 15 is started by the controller to drive the support seat 16 to move upward, thereby driving the sterilized items placed on the placement rack 3 to move up to the operating room 101. A baffle door is provided at the connection between the operating room 101 and the transfer bin 2. When the items are to be transferred, the baffle door is opened. After the items are transferred to the operating room 101, the telescopic rod 15 is started by the controller to drive the support seat 16 to move down and reset to the transfer bin 2, and then the baffle door is closed, thereby completing the transfer of the items.

[0038] In a further preferred embodiment of the present invention, a rotating mechanism is provided on the support seat 16 for driving the items placed on the placement rack 3 to rotate, and the rotating mechanism includes: a mounting groove 17 opened on the support seat 16, and a driving motor 18 is fixedly installed in the mounting groove 17; a rotating shaft 19 rotatably installed on the support seat 16, the bottom end of the rotating shaft 19 is fixedly connected to the output shaft of the driving motor 18, and the other end of the rotating shaft 19 is connected to the bottom of the placement rack 3.

[0039] In this embodiment, when the atomizing nozzle 5 atomizes the hydrogen peroxide solution into the transfer bin 2 and sterilizes the items placed on the placement rack 3, the controller simultaneously starts the drive motor 18 to drive the rotating shaft 19 to rotate. The rotation speed of the drive motor 18 can be set and controlled according to actual needs. This is the prior art and will not be elaborated here. The rotating shaft 19 drives the placement rack 3 to rotate, thereby rotating the items placed on the placement rack 3. The continuously rotating items can better contact the atomized hydrogen peroxide solution in the transfer bin 2, thereby further making the items placed on the placement rack 3 evenly covered with the hydrogen peroxide solution, thereby further improving the sterilization effect on the items.

[0040] In a further preferred embodiment of the present invention, a connecting mechanism is provided on the rotating shaft 19 and the placing rack 3, and the connecting mechanism is used to connect and fix the rotating shaft 19 and the placing rack 3, and the connecting mechanism includes: a connecting seat 20 fixedly installed on the top of the rotating shaft 19; a connecting groove 21 opened on the connecting seat 20; a connecting block 22 fixedly installed on the bottom of the placing rack 3, and the connecting block 22 and the connecting groove 21 are adapted to be connected; and a fixing mechanism assembled on the connecting seat 20 for fixing the connecting block 22 in the connecting groove 21.

[0041] In this embodiment, when the placement rack 3 and the rotating shaft 19 are connected, the connecting block 22 at the bottom of the placement rack 3 is placed in the connecting groove 21 on the connecting seat 20, and then the connecting block 22 is fixed in the connecting groove 21 by the fixing mechanism, so that the placement rack 3 is fixed on the top of the rotating shaft 19. At the same time, when it is necessary to fully clean the transfer bin 2 and the placement rack 3, the fixing mechanism is operated to disengage the fixing of the connecting block 22, so that the connecting block 22 can be removed from the connecting seat 20, so that the placement rack 3 can be disassembled, thereby facilitating the staff to fully clean the transfer bin 2 and the placement rack 3, providing convenience for the staff's cleaning work.

[0042] In a further preferred embodiment of the present invention, the fixing mechanism includes: a mounting frame 23 fixedly mounted on one side of the connecting seat 20, and two placement cavities 24 are provided on the mounting frame 23; two positioning rods 25 slidably mounted on the mounting frame 23 and the connecting seat 20; two positioning grooves 26 provided on the connecting block 22, and the two positioning grooves 26 are respectively adapted to be connected to the ends of the two positioning rods 25; two stoppers 27 respectively fixedly mounted on the two positioning rods 25, and the two stoppers 27 are respectively slidably connected to the inner walls of the two placement cavities 24; two springs 28 respectively mounted on the two positioning rods 25; and an operating frame 29 fixedly mounted on the two positioning rods 25 for pulling the two positioning rods 25.

[0043] In this embodiment, when connecting the placement rack 3 and the rotating shaft 19, the staff first pulls the operating rack 29 to drive the two positioning rods 25 to move, and the two positioning rods 25 respectively drive the two stoppers 27 to compress the two springs 28. As the positioning rods 25 move, the ends of the two positioning rods 25 are moved out of the connecting grooves 21, and then the connecting block 22 at the bottom of the placement rack 3 is placed in the connecting groove 21 on the connecting seat 20. Then the staff releases the operating rack 29, and the spring 28 is reset at this time, so that the ends of the two positioning rods 25 are respectively connected to the two positioning grooves 26 on the connecting block 22, and the connecting block 22 is fixed in the connecting groove 21. The placement rack 3 is fixed on the top of the rotating shaft 19. When the placement rack 3 and the rotating shaft 19 need to be disassembled, the staff first pulls the operating frame 29 to drive the two positioning rods 25 to move, and the two positioning rods 25 respectively drive the two stoppers 27 to compress the two springs 28. As the positioning rods 25 move, the ends of the two positioning rods 25 are respectively disconnected from the two positioning grooves 26 on the connecting block 22, and then the connecting block 22 is removed from the connecting seat 20, so that the placement rack 3 can be disassembled, which makes it easier for the staff to fully clean the transfer bin 2 and the placement rack 3, providing convenience for the staff's cleaning work.

[0044] In a further preferred embodiment of the present invention, a plurality of sliders 30 are fixedly mounted on the bottom of the placement rack 3, a slide groove 31 is provided on the top of the support seat 16, the slide groove 31 is arranged in an annular shape, and the plurality of sliders 30 and the slide groove 31 are slidably connected.

[0045] In this embodiment, when the rotating shaft 19 drives the placement rack 3 to rotate, the placement rack 3 drives the slider 30 to rotate in the slide groove 31, thereby improving the stability of the placement rack 3 during rotation, and when the placement rack 3 is disassembled, the placement rack 3 can be pulled upward to disconnect the slider 30 and the slide groove 31.

[0046] In a further preferred embodiment of the present invention, a stirring mechanism for stirring the hydrogen peroxide solution placed in the storage chamber 10 is provided in the storage chamber 10, and the stirring mechanism includes: two placement shafts 32 rotatably installed in the storage chamber 10, and a plurality of stirring frames 33 for stirring the hydrogen peroxide solution placed in the storage chamber 10 are fixedly installed on the two placement shafts 32; two second bevel gears 34 are respectively fixedly mounted on the installation shaft 8 and one of the placement shafts 32, and the two second bevel gears 34 are meshed with each other; two synchronous wheels 35 are respectively fixedly mounted on the two placement shafts 32, and the two synchronous wheels 35 are provided with synchronous belts 36.

[0047] In this embodiment, when the adjustment mechanism adjusts the spraying angle of the atomizing nozzle 5, as the mounting shaft 8 rotates, under the action of the two second bevel gears 34, one of the placement shafts 32 is driven to rotate, and under the action of the two synchronous wheels 35 and the synchronous belt 36, the other placement shaft 32 is driven to rotate. At this time, the two placement shafts 32 drive multiple stirring racks 33 to rotate, and the rotating stirring racks 33 stir the hydrogen peroxide solution in the storage chamber 10, so that the hydrogen peroxide solution is more uniform, thereby improving the sterilization effect on the objects.

[0048] In a further preferred embodiment of the present invention, a hydrogen peroxide concentration sensor 37 is provided in the transfer chamber 2 , and the hydrogen peroxide concentration sensor 37 is used to monitor the hydrogen peroxide concentration in the transfer chamber 2 .

[0049] In this embodiment, the hydrogen peroxide concentration sensor 37 is used to monitor the hydrogen peroxide concentration in the transfer chamber 2 .

[0050] In summary, compared with the related art, the entire device can be used to culture cells and evenly sterilize the items used, effectively improving the sterilization effect.

[0051] In the several embodiments provided in this application, it should be understood that the disclosed device can be implemented in other ways.

[0052] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the scope of protection of the invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on these embodiments, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in this field can still combine, add, delete or make other adjustments to the features in the various embodiments of the present invention according to the circumstances without conflict, without making creative work, so as to obtain different other technical solutions that do not deviate from the concept of the present invention in essence, and these technical solutions also belong to the scope of protection of the present invention.

Claims

1. A cell culture isolator with transfer sterilization function, characterized in that: include: An isolator body for cell culture, the isolator body comprising a transfer chamber for transferring items used for cell culture; A placement rack provided in the transfer bin for placing items; A shunt pipe disposed in the transfer bin, the shunt pipe being provided with an atomizing nozzle for atomizing a hydrogen peroxide solution into the transfer bin to disinfect the articles; An adjusting mechanism is arranged on the isolator body and the diverter pipe for adjusting the use angle of the atomizing nozzle.

2. The cell culture isolator with transfer sterilization function as claimed in claim 1, characterized in that: The regulating mechanism comprises: An installation cavity is provided on the isolator body, and a dual-axis motor is fixedly installed in the installation cavity; Two installation shafts are rotatably installed in the installation cavity, and the two installation shafts are respectively fixedly connected to the two output shafts of the dual-shaft motor; A plurality of connecting shafts rotatably mounted on the isolator body, wherein the plurality of connecting shafts are respectively fixedly connected to two ends of the two shunt pipes; The four first bevel gears are respectively fixedly mounted on the two connecting shafts and the two mounting shafts, and the two first bevel gears fixedly mounted on the two connecting shafts are respectively meshed with the two first bevel gears fixedly mounted on the two mounting shafts.

3. The cell culture isolator with transfer sterilization function as claimed in claim 2, characterized in that: The isolator body is provided with a feeding mechanism for adding hydrogen peroxide solution into the shunt pipe, and the feeding mechanism comprises: A storage cavity for placing a hydrogen peroxide solution is provided on the isolator body, and a feeding port is provided on the isolator body, the feeding port is in communication with the storage cavity, and the feeding port is used to add the hydrogen peroxide solution into the storage cavity; A water pump fixedly mounted on the isolator body; A feed pipe fixedly mounted on the isolator body, the feed pipe being connected to a water inlet end of the water pump, and the feed pipe extending into the storage cavity; A connecting pipe fixedly mounted on an inner wall of one side of the transfer bin, the connecting pipe being connected to a water outlet end of the water pump; Two hoses are arranged on the connecting pipe, and the two hoses are respectively connected with the two diversion pipes.

4. The cell culture isolator with transfer sterilization function as claimed in claim 1, characterized in that: The transfer bin is provided with a feeding mechanism for driving the items on the placement rack to move upward for feeding, and the feeding mechanism comprises: A telescopic rod fixedly mounted on the isolator body; A support base is fixedly mounted on the output shaft of the telescopic rod, the support base is connected to the placement rack, and the support base is used to support and place the placement rack.

5. The cell culture isolator with transfer sterilization function as claimed in claim 4, characterized in that: The support seat is provided with a rotating mechanism for driving the items placed on the placement rack to rotate, and the rotating mechanism includes: A mounting groove is provided on the support seat, and a driving motor is fixedly installed in the mounting groove; A rotating shaft is rotatably mounted on the support seat, wherein the bottom end of the rotating shaft is fixedly connected to the output shaft of the driving motor, and the other end of the rotating shaft is connected to the bottom of the placement rack.

6. The cell culture isolator with transfer sterilization function as claimed in claim 5, characterized in that: The rotating shaft and the placing rack are provided with a connecting mechanism, and the connecting mechanism is used to connect and fix the rotating shaft and the placing rack, and the connecting mechanism includes: A connecting base fixedly mounted on the top of the rotating shaft; A connecting groove is provided on the connecting seat; A connecting block fixedly mounted on the bottom of the placement rack, wherein the connecting block is adapted to be connected with the connecting groove; A fixing mechanism is mounted on the connecting seat and is used to fix the connecting block in the connecting groove.

7. The cell culture isolator with transfer sterilization function as claimed in claim 6, characterized in that: The fixing mechanism comprises: A mounting frame fixedly mounted on one side of the connecting seat, wherein the mounting frame has two placement cavities; Two positioning rods slidably mounted on the mounting frame and the connecting seat; Two positioning grooves are provided on the connection block, and the two positioning grooves are respectively adapted to be connected with the ends of the two positioning rods; Two stoppers are respectively fixedly sleeved on the two positioning rods, and the two stoppers are respectively slidably connected to the inner walls of the two placement cavities; Two springs respectively sleeved on the two positioning rods; An operating frame is fixedly mounted on the two positioning rods and is used for pulling the two positioning rods.

8. The cell culture isolator with transfer sterilization function as claimed in claim 6, characterized in that: A plurality of sliding blocks are fixedly mounted on the bottom of the placement rack, a sliding groove is provided on the top of the support seat, the sliding groove is annularly arranged, and a plurality of the sliding blocks are slidably connected to the sliding groove.

9. The cell culture isolator with transfer sterilization function as claimed in claim 3, characterized in that: The storage chamber is provided with a stirring mechanism for stirring the hydrogen peroxide solution placed in the storage chamber, and the stirring mechanism comprises: Two placement shafts rotatably mounted in the storage chamber, wherein a plurality of stirring racks for stirring the hydrogen peroxide solution placed in the storage chamber are respectively fixedly mounted on the two placement shafts; Two second bevel gears are respectively fixedly sleeved on the installation shaft and one of the placement shafts, and the two second bevel gears are meshed with each other; Two synchronous wheels are respectively fixedly sleeved on the two placement shafts, and synchronous belts are sleeved on the two synchronous wheels.

10. The cell culture isolator with transfer sterilization function as claimed in claim 1, characterized in that: A hydrogen peroxide concentration sensor is arranged in the transfer bin, and the hydrogen peroxide concentration sensor is used to monitor the hydrogen peroxide concentration in the transfer bin.