Protein purification liquid collecting device

Abstract

The utility model discloses a protein purification liquid collecting device, which relates to the technical field of protein purification liquid collection and comprises two power mechanisms arranged at the top of a bottom plate in parallel, each power mechanism comprises a motor and a worm, and the other end of each worm is rotatably connected to a base; the two sides of the bottom plate are each provided with a sliding groove, the supporting frame comprises a flat plate and two vertical rods arranged at the bottom of the flat plate, the bottoms of the vertical rods are in sliding fit with the sliding grooves, the movable frame is in sliding fit with the top of the flat plate, a rack is arranged on one inner wall of the movable frame, and a long shaft of the rack is perpendicular to a long shaft of the worm. The gear is coaxially connected with a worm wheel through a rotating rod, the rotating rod penetrates through the flat plate, the worm wheel is meshed with the two worms, and the gear is meshed with the rack; a mounting plate is arranged on the top surface of the moving frame, a test tube rack is arranged at the top of the mounting plate, the long axis of the test tube rack is parallel to the long axis of the worm, and test tubes are used for collecting liquid released by the chromatographic column. According to the scheme, the test tube rack can move in two mutually perpendicular directions, so that each test tube can be used for collecting impurities or target proteins.

Description

Technical Field

[0001] The utility model relates to the technical field of protein purification liquid collection, in particular to a protein purification liquid collection device. Background Art

[0002] The principle of separation and purification of proteins by chromatography system is that proteins interact with stationary phases to different extents under different conditions. Specifically, the chromatography system includes two main steps: adsorption chromatography and elution chromatography. In adsorption chromatography, the target protein is adsorbed by the functional groups on the surface of the stationary phase, while non-target substances are washed away. Then, by changing the conditions, such as adjusting the solvent composition or pH value, the adsorbed protein can be gradually eluted to obtain the target protein.

[0003] The method for collecting impurities and target proteins in the chromatography system is: a slide that can move in a straight line is set under the chromatography column, and a test tube rack is set on the slide. Test tubes are placed in the test tube rack. By moving the slide, different test tubes can be moved under the chromatography column to collect impurities or target proteins. The problem is that the slide can only move in a straight line, which means that only one row of test tubes in the test tube rack can be used to collect impurities or target proteins, so the staff needs to frequently place empty test tubes in the test tube rack, which affects the efficiency of the experiment. Utility Model Content

[0004] In order to solve the deficiencies of the prior art, the utility model provides a protein purification liquid collection device, which can enable a test tube rack to move in two mutually perpendicular directions, thereby ensuring that each test tube can be used to collect impurities or target proteins.

[0005] In order to achieve the purpose of this utility model, the following scheme is proposed:

[0006] A protein purification liquid collection device is arranged below a chromatography column of a protein purifier and comprises two power mechanisms, a support frame, a moving frame, gears, a worm gear and a test tube rack.

[0007] Two power mechanisms are arranged in parallel on the top of the bottom plate, each power mechanism comprises a motor and a worm connected to its output shaft, and the other end of the worm is rotatably connected to the base;

[0008] A slide groove is arranged on each side of the bottom plate, and the support frame includes a flat plate and two vertical rods arranged at the bottom thereof, the bottom of the vertical rods are slidably matched with the corresponding slide grooves, and the moving frame is slidably matched with the top of the flat plate, and a rack is arranged on one inner wall of the moving frame, and the long axis of the rack is perpendicular to the long axis of the worm;

[0009] The gear is coaxially connected to the worm wheel through a rotating rod, the rotating rod passes through the plate, the worm wheel is meshed with two worms, and the gear is meshed with the rack;

[0010] A mounting plate is arranged on the top of the movable frame, and a test tube rack is arranged on the top of the mounting plate. The long axis of the test tube rack is parallel to the long axis of the worm. The test tube rack is used to place the test tube, and the test tube is used to collect the liquid released by the chromatography column.

[0011] Furthermore, an I-shaped block is provided at the bottom of the vertical rod, and the I-shaped block is slidably matched in the corresponding sliding groove.

[0012] Furthermore, the bottom surface of the gear is in close contact with the flat plate.

[0013] Furthermore, two baffles are arranged on the top of the flat plate, the movable frame is located between the two baffles, and the outer wall of the movable frame is closely attached to the inner wall of the baffle.

[0014] Furthermore, the test tube rack is fixed to the mounting plate by a plurality of screws.

[0015] The beneficial effects of the utility model are that multiple rows and columns of test tubes can be placed on the test tube rack, and the test tube rack can be moved in two directions through the ingenious cooperation of the power mechanism, the support frame, the moving frame, the gears, and the worm gear, thereby ensuring that each test tube can be used to collect impurities or target proteins released by the chromatography column, and the staff does not need to frequently place empty test tubes in the test tube rack, thereby improving the experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The structure diagram of the protein purification liquid collection device is shown;

[0017] Figure 2 A front view of a protein purification liquid collection device is shown;

[0018] Figure 3 Shown is a top view of the interior of the supporting frame and the moving frame. DETAILED DESCRIPTION

[0019] like Figure 1 As shown, this embodiment provides a protein purification liquid collection device, which is arranged below the chromatography column of the protein purifier 8 and is used to collect the liquid released by the chromatography column. The device includes two power mechanisms 1, a support frame, a moving frame 3, a gear 4, a worm gear 5, and a test tube rack 7.

[0020] Specifically, two power mechanisms 1 are arranged in parallel on the top of the base plate 6 , and each power mechanism 1 includes a motor 11 and a worm 12 . The output shaft of the motor 11 is connected to one end of the worm 12 , and the other end of the worm 12 is rotatably connected to a base, which is arranged on the top of the base plate 6 .

[0021] Specifically, Figure 1 , Figure 3As shown, a slide groove 61 is arranged on each side of the bottom plate 6, and the long axis of the slide groove 61 is parallel to the long axis of the worm 12. The supporting frame includes a flat plate 21 and two vertical rods 22. The two vertical rods 22 are symmetrically arranged on the top of the flat plate 21. An I-shaped block 23 is arranged at the bottom of the vertical rod 22. The I-shaped block 23 is slidably matched with the corresponding slide groove 61. The moving frame 3 is a rectangular frame. Two baffles 211 are arranged on the top of the flat plate 21. The moving frame 3 is located between the two baffles 211. The outer wall of the moving frame 3 is close to the inner wall of the baffle 211. The moving frame 3 is slidably matched with the limiting groove surrounded by the two baffles 211. A rack 31 is arranged on one of the inner walls of the moving frame 3. The long axis of the rack 31 is perpendicular to the long axis of the worm 12.

[0022] Specifically, Figure 1 , Figure 3 As shown, the gear 4 is coaxially connected to the worm wheel 5 through a rotating rod 41, the rotating rod 41 passes through the plate 21, the rotating rod 41 rotates with the through hole, the worm wheel 5 is meshed with the two worms 12 at the same time, the gear 4 is located in the moving frame 3, the gear 4 is meshed with the rack 31, and in order to improve the structural stability, the bottom surface of the gear 4 is close to the plate 21.

[0023] The principle is as follows: Figure 3 From the perspective, when the two motors 11 drive their respective worms 12 in opposite directions, the worm wheel 5 will be caused to rotate in place, and then the gear 4 will be caused to rotate. Because the gear 4 engages the rack 31, the gear 4 will cause the moving frame 3 to slide along the flat plate 21, and the sliding direction of the moving frame 3 is perpendicular to the long axis of the worm 12; when the two motors 11 drive their respective worms 12 in the same direction, the worm wheel 5 will not rotate, but will move in a straight line in the direction of the motor 11, which means that the support frame and the moving frame 3 move in a straight line with the worm wheel 5; because the worm wheel 5 does not rotate, the gear 4 does not rotate, and the moving frame 3 will not slide along the flat plate 21.

[0024] Specifically, a mounting plate 32 is fixedly provided on the top surface of the movable frame 3, and a test tube rack 7 is provided on the top of the mounting plate 32. The long axis of the test tube rack 7 is parallel to the long axis of the worm 12. The test tube rack 7 is used to place a test tube, and the test tube is used to collect liquid released by the chromatography column. More specifically, in order to facilitate the installation or replacement of the test tube rack 7, the test tube rack 7 is fixed to the mounting plate 32 by a plurality of screws.

[0025] The principle is as follows: In this embodiment, the test tube rack 7 is provided with 9 rows and 3 columns of test tube holes. For the sake of description, it is assumed that the test tube hole at the 1st column of the 1st row is N11, and by analogy, the test tube hole at the 3rd column of the 9th row is N93. First, N11 is moved to the bottom of the chromatography column. After N11 collects the liquid, the two motors 11 drive their respective worms 12 in reverse, causing the moving frame 3 and the test tube rack 7 to slide along the flat plate 21 as a whole, thereby moving N12 to the bottom of the chromatography column. After N11 collects the liquid, the two motors 11 continue to drive their respective worms 12 in reverse. , thereby moving N13 to the bottom of the chromatography column; after the test tubes in the first row have collected the liquid, the two motors 11 drive their respective worms 12 in the same direction, which will cause the support frame, the moving frame 3 and the test tube rack 7 to move linearly in the direction of the motor 11 as a whole, thereby moving N23 to the bottom of the chromatography column; the staff can take away the test tubes in the first row for the next operation. After N23 collects the liquid, the two motors 11 change to another way to reversely drive their respective worms 12, thereby moving N22 to the bottom of the chromatography column, and the cycle continues in sequence, which will not be described in detail here.

[0026] In the above scheme, the test tube rack 7 can be moved in two directions to ensure that all 27 test tubes distributed in the array can be used to collect impurities or target proteins. The staff does not need to frequently place empty test tubes in the test tube rack 7, thereby improving the experimental efficiency.

[0027] The above embodiments are only used to illustrate the technical ideas and features of the utility model, and are not intended to be the only or limit the utility model. Those skilled in the art should understand that various changes or equivalent replacements made to the utility model without departing from the scope of the utility model are within the scope of protection of the utility model.

Claims

1. A protein purification liquid collection device, characterized in that: It is arranged below the chromatography column of the protein purification instrument (8), and comprises two power mechanisms (1), a support frame, a moving frame (3), a gear (4), a worm gear (5), and a test tube rack (7); Two power mechanisms (1) are arranged in parallel on the top of the bottom plate (6), each power mechanism (1) comprises a motor (11) and a worm (12) connected to its output shaft, and the other end of the worm (12) is rotatably connected to the base; A slide groove (61) is provided on each side of the bottom plate (6), the support frame comprises a flat plate (21) and two vertical rods (22) provided at the bottom thereof, the bottoms of the vertical rods (22) are slidably matched with the corresponding slide grooves (61), the moving frame (3) is slidably matched with the top of the flat plate (21), one inner wall of the moving frame (3) is provided with a rack (31), and the long axis of the rack (31) is perpendicular to the long axis of the worm (12); The gear (4) is coaxially connected to the worm wheel (5) via a rotating rod (41), the rotating rod (41) passes through the plate (21), the worm wheel (5) is meshed with the two worms (12), and the gear (4) is meshed with the rack (31); A mounting plate (32) is provided on the top surface of the movable frame (3), and a test tube rack (7) is provided on the top of the mounting plate (32). The long axis of the test tube rack (7) is parallel to the long axis of the worm (12). The test tube rack (7) is used to place a test tube, and the test tube is used to collect liquid released by the chromatography column.

2. The protein purification liquid collection device according to claim 1, characterized in that: An I-shaped block (23) is provided at the bottom of the vertical rod (22), and the I-shaped block (23) is slidably matched in the corresponding sliding groove (61).

3. The protein purification liquid collection device according to claim 1, characterized in that: The bottom surface of the gear (4) is in close contact with the flat plate (21).

4. The protein purification liquid collection device according to claim 1, characterized in that: Two baffles (211) are provided on the top of the flat plate (21), the movable frame (3) is located between the two baffles (211), and the outer wall of the movable frame (3) is in close contact with the inner wall of the baffle (211).

5. The protein purification liquid collection device according to claim 1, characterized in that: The test tube rack (7) is fixed to the mounting plate (32) by means of a plurality of screws.

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