Efficient culture device for arbuscular mycorrhizal fungi single spore
By designing an efficient culture device including through holes and bottom isolation disks, the problem of low success rate of monospores of arboric mycorrhizal fungi is solved, and a higher success rate of infection and a more efficient culture process is achieved.
Patent Information
- Application Number
- CN202421203213.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-29
- Publication Date
- 2025-02-25
- Estimated Expiration
- 2034-05-29
AI Technical Summary
In the prior art, the success rate of isolating monospores of arbuscular mycorrhizal fungi is low, mainly because the roots of adjacent planting holes are prone to cross-contamination, and the space limitations of seedling trays or flower pots are not conducive to spore production and mycelial infection.
An efficient culture device including the first stent plate and the second stent plate is designed. Through the design of the through hole and the bottom isolation disc, the independent arrangement and rotation of the culture unit is realized, avoiding the cross-contamination of the root system, and driving through the belt connection. The bottom isolation disc rotates to ensure that the spores are evenly exposed to sunlight.
The success rate of infection of arbuscular mycorrhizal fungi monospores is improved, the risk of spore cross-infection is reduced, space is saved, and the number of watering is reduced through the water storage function is improved, and the culture efficiency is improved.
Smart Images
Figure CN222524518U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of fungus cultivation, in particular to a high-efficiency cultivation device for arbuscular mycorrhizal fungi monospores. Background Art
[0002] Arbuscular mycorrhizal fungi (AMF) are an important beneficial soil microorganism that can establish a symbiotic relationship with more than 80% of terrestrial plants. After establishing a symbiotic relationship, the hyphal network of AM fungi extends into the soil, greatly increasing the surface area of the absorbing roots, improving the plant's acquisition of soil nutrients, enhancing the plant's tolerance to surrounding environmental stress, and promoting plant growth.
[0003] At present, the common method for isolating AMF strains is to select single spores from the soil, then inoculate the spores onto the plant roots, and cultivate them together in a seedling tray or flower pot filled with a sterilized culture matrix (such as soil or fine sand) for 2-3 months, in the hope that the spore inoculation is successful and the spores are reproduced. This planting method of seedling trays or flower pots usually has a very low success rate. On the one hand, the roots of adjacent planting holes are easily entangled with each other through the bottom drainage holes, resulting in cross-contamination of hyphae produced by different spores, and the goal of single spore separation cannot be strictly achieved. On the other hand, either the root growth space of the seedling tray is too small to produce spores, or the scattered roots in the flower pot are not conducive to AMF infection. For this reason, we proposed an efficient culture device for single spores of arbuscular mycorrhizal fungi. Utility Model Content
[0004] In order to solve the problem that the current isolation of strains generally involves selecting spores and then grafting the spores to the root system of plants and cultivating them in seedling pots or flower pots, which not only leads to cross-contamination of mycelium produced by different spores, but also is not conducive to spore production due to too small a space or is not conducive to AMF infection due to dispersed root systems, the utility model provides the following technical solutions: an efficient culture device for single spores of arbuscular mycorrhizal fungi, comprising a first support plate and a second support plate, the first support plate and the second support plate being detachably connected, a through hole being provided on the surface of the first support plate, a bottom isolation plate being provided on the top of the second support plate, the bottom isolation plate corresponding to the through hole, and the through hole and the bottom isolation plate both having multiple and consistent numbers; and a culture unit, the bottom of the culture unit being able to pass through the through hole to insert into the bottom isolation plate, the bottom of the culture unit being provided with a first drainage hole, and the bottom of the second support plate being connected to a heightening frame.
[0005] Preferably, a card slot is provided at the bottom of the first bracket plate, and a column is provided at the top of the second bracket plate, and the column can be detachably inserted into the card slot.
[0006] Preferably, the center of the bottom isolation disk is rotatably connected to the second bracket plate, and the bottom isolation disks in the same row are circumferentially connected by belts. There is friction between the bottom isolation disk and the belts. A protrusion is provided on the outside of the belt, a recess is provided at the bottom of the culture unit, and a protrusion is provided inside the bottom isolation disk. The protrusion and the recess can be matched and plugged in, and the bottom isolation disk can be rotated by pushing the protrusion on the outside of the belt.
[0007] Preferably, a second drainage hole is provided at the bottom of the second bracket plate, the second drainage hole is smaller than the through hole, the second drainage holes are consistent in size, and the second drainage hole is conducive to drainage, maintaining the bracket dry and reducing the growth of bacteria.
[0008] Preferably, the culture unit is made of glass, acrylic or polypropylene with better light transmittance.
[0009] Preferably, the distance between the plurality of through holes is 1-1.5 cm, and the compact arrangement greatly saves space and improves the success rate of single spore infection per unit space.
[0010] Compared with the prior art, the beneficial effects of the utility model are:
[0011] A plurality of culture units are placed between the through hole and the bottom isolation plate, the bottom recess of the culture unit is matched and plugged with the protrusion in the bottom isolation plate, the center of the bottom isolation plate is rotatably connected to the second bracket plate, and the bottom isolation plate is circumferentially connected by a belt, and the protrusions on the outer side of the belt are pushed to drive the bottom isolation plate and the culture unit to rotate through friction, so as to ensure that the single spores of mycorrhizal fungi are evenly exposed to sunlight, and the first drainage hole can avoid cross-infection of spores of adjacent planting tubes, ensure smooth drainage without water accumulation in the culture unit, and the bottom isolation plate ensures that there is water stored at the bottom of the culture unit for short-term use, thereby reducing the number of watering times, and the through holes are compactly arranged to save space, thereby improving the success rate of single spore infection per unit space. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:
[0013] Figure 1 It is a front view structural schematic diagram of the utility model as a whole;
[0014] Figure 2 It is a schematic diagram of the overall structure of the utility model from top view;
[0015] Figure 3 This is an enlarged schematic diagram of the bottom of the cultivation unit of the utility model;
[0016] In the figure: 1. first bracket plate; 2. slot; 3. bottom isolation plate; 4. column; 5. belt; 6. bump; 7. through hole; 8. second drainage hole; 9. culture unit; 10. first drainage hole; 11. heightening frame; 12. second bracket plate. DETAILED DESCRIPTION
[0017] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments; based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.
[0018] Depend on Figure 1-3 It is given that the utility model includes a first bracket plate 1 and a second bracket plate 12, the first bracket plate 1 and the second bracket plate 12 are detachably connected, a through hole 7 is opened on the surface of the first bracket plate 1, a bottom isolation plate 3 is arranged on the top of the second bracket plate 12, the bottom isolation plate 3 corresponds to the through hole 7, and there are multiple through holes 7 and the bottom isolation plate 3 and the number is consistent; it also includes a culture unit 9, the bottom of the culture unit 9 can be inserted into the bottom isolation plate 3 through the through hole 7, a first drainage hole 10 is opened at the bottom of the culture unit 9, and the bottom of the second bracket plate 12 is connected to the raising frame 11.
[0019] A slot 2 is provided at the bottom of the first bracket plate 1 , and a column 4 is provided at the top of the second bracket plate 12 . The column 4 can be detachably inserted into the slot 2 .
[0020] The center of the bottom isolation disk 3 is rotatably connected to the second bracket plate 12, and the bottom isolation disks 3 in the same row are circumferentially connected by belts 5. There is friction between the bottom isolation disk 3 and the belts 5. A protrusion 6 is provided on the outside of the belt 5, a recess is provided at the bottom of the culture unit 9, and a protrusion is provided inside the bottom isolation disk 3. The protrusion and the recess can be matched and plugged in, and the bottom isolation disk 3 can be rotated by pushing the protrusion 6 on the outside of the belt 5.
[0021] The second drain hole 8 is provided at the bottom of the second bracket plate 12. The second drain hole 8 is smaller than the through hole 7 and has the same size. The second drain hole 8 is conducive to drainage, maintaining the bracket dry and reducing the growth of bacteria.
[0022] The culture unit 9 is made of glass, acrylic or polypropylene with better light transmittance.
[0023] The distance between the multiple through holes 7 is 1-1.5 cm, and the compact arrangement greatly saves space and improves the success rate of single spore infection per unit space.
[0024] Working principle: multiple culture units 9 are placed between the through hole 7 and the bottom isolation plate 3, the bottom recess of the culture unit 9 is matched and plugged with the protrusion in the bottom isolation plate 3, the center of the bottom isolation plate 3 is rotatably connected to the second bracket plate 12, and the bottom isolation plate 3 is connected circumferentially by the belt 5, and the outer protrusion 6 of the belt 5 is pushed to drive the bottom isolation plate 3 and the culture unit 9 to rotate through friction, so as to ensure that the single spores of arbuscular mycorrhizal fungi are evenly exposed to sunlight, and the first drainage hole 10 can avoid cross-infection of spores of adjacent planting tubes, ensure smooth drainage without water accumulation in the culture unit 9, and the bottom isolation plate 3 ensures that there is water storage at the bottom of the culture unit 9 for short-term use, thereby reducing the number of watering times, and the through holes 7 are compactly arranged to save space, thereby improving the success rate of single spore infection per unit space, and the bottom of the second bracket plate 12 is connected to a heightening frame to ensure growth space for the roots of arbuscular mycorrhizal fungi.
[0025] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.
[0026] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. An efficient culture device for arbuscular mycorrhizal fungi monospores, comprising a first support plate (1) and a second support plate (12), characterized in that: The first support plate (1) is detachably connected to the second support plate (12); a through hole (7) is provided on the surface of the first support plate (1); a bottom isolation plate (3) is provided on the top of the second support plate (12); the bottom isolation plate (3) corresponds to the through hole (7); there are a plurality of the through holes (7) and the bottom isolation plate (3) and the number of the through holes (7) and the bottom isolation plate (3) are consistent; the support plate also includes a culture unit (9); the bottom of the culture unit (9) can pass through the through hole (7) and be inserted into the bottom isolation plate (3); a first drainage hole (10) is provided on the bottom of the culture unit (9); and the bottom of the second support plate (12) is connected to a heightening frame (11).
2. The efficient culture device for single spores of arbuscular mycorrhizal fungi according to claim 1, characterized in that: The first bracket plate (1) has a card slot (2) at the bottom, and the second bracket plate (12) has a column (4) at the top. The column (4) can be detachably inserted into the card slot (2).
3. The efficient culture device for single spores of arbuscular mycorrhizal fungi according to claim 1, characterized in that: The center of the bottom isolation disk (3) is rotatably connected to the second support plate (12), and the bottom isolation disks (3) in the same row are circumferentially connected by a belt (5). There is friction between the bottom isolation disk (3) and the belt (5). A protrusion (6) is arranged on the outside of the belt (5), a recess is arranged at the bottom of the culture unit (9), and a protrusion is arranged inside the bottom isolation disk (3), and the protrusion and the recess can be matched and plugged.
4. The efficient culture device for single spores of arbuscular mycorrhizal fungi according to claim 1, characterized in that: A second drainage hole (8) is provided at the bottom of the second bracket plate (12); the second drainage hole (8) is smaller than the through hole (7); and the second drainage holes (8) are of the same size.
5. The efficient culture device for single spores of arbuscular mycorrhizal fungi according to claim 1, characterized in that: The culture unit (9) is made of glass, acrylic or polypropylene.
6. The efficient culture device for single spores of arbuscular mycorrhizal fungi according to claim 1, characterized in that: The distance between the plurality of through holes (7) is 1-1.5 cm.