Synchronous separation system for cow milk protein components

Abstract

The utility model relates to the technical field of cow milk protein separation and purification, and provides a cow milk protein component synchronous separation system. Comprising a milk bin, a fat separator, a first cation exchange device, a first membrane filtration module, a casein solution treatment branch, a second membrane filtration module, a second cation exchange device, a first anion exchange device, a second anion exchange device, a purification and sterilization treatment branch and a product production line. Membrane filtration and a chromatographic technology are combined, and different active proteins are separated by using the molecular weight, the charged property, the hydrophobic interaction and the like of the proteins. Main active protein in milk can be separated, beta-casein, alpha-lactalbumin, immune globulin and lactoferrin can be sequentially separated and purified from raw milk, drying or sterile filling is carried out, different products are prepared while natural nutritional ingredients are reserved, and in addition, the product quality is improved. The separated milk can still be processed into sterilized milk or milk beverages.

Description

Technical Field

[0001] The utility model relates to the technical field of separation and purification of milk protein, in particular to a synchronous separation system of milk protein components. Background Art

[0002] Milk protein, also known as bovine milk protein, is a general term for a mixture of many kinds of proteins in milk. It is mainly composed of two parts: casein and whey protein. Whey protein is a water-soluble protein, composed of components such as α-lactalbumin and β-lactoglobulin. α-Lactalbumin contains a large amount of amino acids such as leucine and isoleucine that are essential to the human body but cannot be synthesized by itself. Casein includes components such as α-casein, β-casein and κ-casein. Casein has physiological effects on dental caries, prevention and treatment of osteoporosis and rickets, regulation of blood pressure, treatment of iron deficiency anemia, magnesium deficiency neuritis and many other diseases. However, in the related art, the method of separating bovine milk protein is generally aimed at separating a certain component of bovine milk alone, and there is no systematic separation of multiple active proteins in milk. Utility Model Content

[0003] The utility model provides a milk protein component synchronous separation system, which is used to solve or improve the existing problem that multiple active proteins in milk cannot be separated simultaneously.

[0004] The utility model provides a synchronous separation system for milk protein components, comprising: a milk silo, a fat separator, a first cation exchange device, a first membrane filtration module, a casein solution processing branch, a second membrane filtration module, a second cation exchange device, a first anion exchange device, a second anion exchange device, a purification and sterilization processing branch and a product production line;

[0005] The milk silo, the fat separator and the first cation exchange device are connected in sequence, the first outlet of the first cation exchange device is connected to the first membrane filtration module, the first outlet of the first membrane filtration module is connected to the casein solution processing branch, the second outlet of the first membrane filtration module is connected to the second membrane filtration module, the first outlet of the second membrane filtration module is connected to the second cation exchange device, the first outlet of the second cation exchange device is connected to the first anion exchange device, and the second outlet of the second cation exchange device is connected to the second anion exchange device;

[0006] Among them, the second outlet of the first cation exchange device, the second outlet of the second membrane filtration module, the first anion exchange device and the second anion exchange device are all connected to the purification and sterilization treatment branch, and the purification and sterilization treatment branch is connected to the product production line.

[0007] According to a milk protein component synchronous separation system provided by the utility model, the milk protein component synchronous separation system also includes a heat exchanger and a first material temporary storage tank, and the first outlet of the first cation exchange device is connected to the first membrane filtration module through the heat exchanger and the first material temporary storage tank.

[0008] According to a synchronous separation system of milk protein components provided by the utility model, the casein solution processing branch includes a second material temporary storage tank, a material mixer, a UHT sterilizer and a filler connected in sequence, and the first outlet of the first membrane filtration module is connected to the second material temporary storage tank.

[0009] According to a milk protein component synchronous separation system provided by the utility model, the milk protein component synchronous separation system further includes a third material temporary storage tank, and the second outlet of the first membrane filtration module is connected to the second membrane filtration module through the third material temporary storage tank; and / or,

[0010] A fourth material temporary storage tank, wherein the second outlet of the second membrane filtration module is connected to the purification and sterilization treatment branch through the fourth material temporary storage tank.

[0011] According to a milk protein component synchronous separation system provided by the utility model, the milk protein component synchronous separation system also includes a fifth material temporary storage tank, and the first outlet of the second membrane filtration module is connected to the second cation exchange device through the fifth material temporary storage tank.

[0012] According to a synchronous separation system of milk protein components provided by the utility model, the synchronous separation system of milk protein components also includes a sixth material temporary storage tank and a seventh material temporary storage tank, the first outlet of the second cation exchange device is connected to the first anion exchange device through the sixth material temporary storage tank, and the first anion exchange device is connected to the purification and sterilization treatment branch through the seventh material temporary storage tank.

[0013] According to a synchronous separation system of milk protein components provided by the utility model, the synchronous separation system of milk protein components also includes an eighth material temporary storage tank and a ninth material temporary storage tank, the second outlet of the second cation exchange device is connected to the second anion exchange device through the eighth material temporary storage tank, and the second anion exchange device is connected to the purification and sterilization treatment branch through the ninth material temporary storage tank.

[0014] According to a milk protein component synchronous separation system provided by the utility model, the milk protein component synchronous separation system also includes a tenth material temporary storage tank, and the second outlet of the first cation exchange device is connected to the purification and sterilization treatment branch through the tenth material temporary storage tank.

[0015] According to a milk protein component synchronous separation system provided by the utility model, the purification and sterilization processing branch comprises a concentration and purification device and a sterilization filter which are connected in sequence.

[0016] According to a milk protein component synchronous separation system provided by the utility model, the product production line includes a freeze drying branch, a spray drying branch and a filling branch connected in parallel.

[0017] Compared with the single process for separating a single active protein, the synchronous separation system of milk protein components provided by the utility model combines membrane filtration with chromatography technology, and separates different active proteins by utilizing the molecular weight, charge property, hydrophobicity, etc. of the protein. That is to say, in this embodiment, the main active proteins in milk can be separated, β-casein, α-lactalbumin, immunoglobulin, and lactoferrin can be separated and purified from the raw milk in sequence, and dried or aseptically filled, and different products can be prepared while retaining the natural nutrients, and the separated milk can still be used to be processed into sterilized milk or milk beverages. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the present invention or the prior art, a brief introduction will be given below to the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0019] Figure 1 The utility model is a schematic diagram of the structure of the synchronous separation system of milk protein components.

[0020] Reference numerals:

[0021] 1. Milk silo; 2. Milk purifier; 3. Fat separator; 4. First cation exchange device; 5. Heat exchanger; 6. First material temporary storage tank; 7. First membrane filtration module; 8. Second material temporary storage tank; 9. Materializer; 10. UHT sterilizer; 11. Filling machine; 12. Third material temporary storage tank; 13. Second membrane filtration module; 14. Fourth material temporary storage tank; 15. Fifth material temporary storage tank; 16. Second cation exchange device; 17. Sixth material temporary storage tank; 18. First anion exchange device; 19. Seventh material temporary storage tank; 20. Eighth material temporary storage tank; 21. Second anion exchange device; 22. Ninth material temporary storage tank; 23. Tenth material temporary storage tank; 24. Purification and sterilization treatment branch; 25. Product production line. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution of the utility model will be clearly and completely described below in conjunction with the drawings in the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] In recent years, with the improvement of living standards, the development of functional foods with bioactive substances as natural raw materials has received more and more attention. Cow's milk is considered to be the best source of natural active substances. The active proteins in cow's milk include lactoferrin, α-lactalbumin, β-lactoglobulin, immunoglobulin, etc.

[0024] At present, these active milk proteins cannot be used well by people. Most of the milk is UHT milk. Due to the high heat intensity of UHT milk, most of the active ingredients are damaged and lose their original efficacy. For example, the lactoferrin content in raw milk is 120ml / L, and the content in the finished product after processing is 0, with a loss rate of 100%; the α-lactalbumin and β-lactoglobulin content in raw milk are 1000mg / L and 3000mg / L respectively, and the content in the finished product after processing is 100mg / L and 300mg / L respectively, with a loss rate of more than 90%.

[0025] How to avoid the heat loss of these milk proteins is a difficult problem currently faced by the dairy industry. With the popularization of membrane filtration and chromatographic separation technology in dairy products, the separation and purification of various active proteins has become possible. Currently, membrane filtration technology is used to separate whey protein from milk and prepare it into high-premium whey protein powder; chromatographic separation technology is also used to extract lactoferrin from milk and add it to room temperature products by aseptic addition. These methods are generally targeted at a certain component of milk, and do not systematically achieve the separation of multiple active proteins in milk.

[0026] In order to solve the above problems, Figure 1 As shown, in an optional embodiment, the synchronous separation system of milk protein components of the utility model embodiment includes: a milk silo 1, a fat separator 3, a first cation exchange device 4, a first membrane filtration module 7, a casein solution processing branch, a second membrane filtration module 13, a second cation exchange device 16, a first anion exchange device 18, a second anion exchange device 21, a purification and sterilization processing branch 24 and a product production line 25.

[0027] The milk silo 1, the fat separator 3 and the first cation exchange device 4 are connected in sequence, and a milk purifier 2 can also be arranged between the milk silo 1 and the fat separator 3. The first outlet of the first cation exchange device 4 is connected to the first membrane filtration module 7, the first outlet of the first membrane filtration module 7 is connected to the casein solution processing branch, the second outlet of the first membrane filtration module 7 is connected to the second membrane filtration module 13, the first outlet of the second membrane filtration module 13 is connected to the second cation exchange device 16, the first outlet of the second cation exchange device 16 is connected to the first anion exchange device 18, and the second outlet of the second cation exchange device 16 is connected to the second anion exchange device 21.

[0028] Among them, the second outlet of the first cation exchange device 4 , the second outlet of the second membrane filtration module 13 , the first anion exchange device 18 and the second anion exchange device 21 are all connected to the purification and sterilization treatment branch 24 , and the purification and sterilization treatment branch 24 is connected to the product production line 25 .

[0029] Fresh milk is collected and stored in the milk silo 1. According to actual needs, the milk silo 1 can be equipped with a stirrer and a cooling device to ensure the freshness and uniformity of the milk. Subsequently, the milk is pumped out of the milk silo 1 and pre-treated by the milk purifier 2 to remove larger particle impurities and insoluble matter therein to ensure the smooth progress of the subsequent processing process. Next, the pre-treated milk enters the fat separator 3. In this step, the fat separator 3 will separate the fat part of the milk from the skim milk by physical centrifugation. Skim milk contains a high protein content and is the main raw material for subsequent separation of protein components.

[0030] Specifically, the raw milk in the milk warehouse 1 enters the milk purifier 2 for pretreatment through a centrifugal pump and a flow meter, and the pretreated milk enters the fat separator 3 to become skimmed milk; the skimmed milk enters the first cation exchange device 4 through a centrifugal pump and a flow meter. After passing through the first cation exchange device 4, the lactoferrin in the milk is adsorbed on the chromatographic column, and a lactoferrin solution is obtained through a chromatographic elution process. The lactoferrin solution enters the product production line 25 through a purification and sterilization treatment branch 24; after the milk without lactoferrin flows through the chromatographic column (flow-through liquid), it enters the first membrane filtration module 7, and is divided into two parts through the first membrane filtration module 7, one part is a retentate casein solution, and the other part is a permeate whey protein solution, and the whey protein solution is rich in β-casein components. The casein solution enters the casein solution treatment branch for making pure milk or milk beverages. The whey protein solution enters the second membrane filtration module 13. The second membrane filtration module 13 can separate the β-casein component in the whey protein, the retentate is the β-casein solution, and the permeate is the whey protein solution; the β-casein solution enters the product production line 25 through the purification and sterilization treatment branch 24; the whey protein solution enters the second cation exchange device 16 through the pump and flowmeter, and the protein content is detected in real time by the ultraviolet protein sensor. The immunoglobulin in the whey protein will be adsorbed on the cationic chromatographic column, while α-lactalbumin and β-lactoglobulin will flow through, and the α-lactalbumin and β-lactoglobulin solutions enter the first anion exchange device 18, in which the α-lactalbumin will be adsorbed on the chromatographic column, while the β-lactoglobulin will flow through. The eluted α-lactalbumin solution enters the product production line 25 through the purification and sterilization treatment branch 24; the immunoglobulin solution enters the second anion exchange device 21, in which the immunoglobulin will be adsorbed on the chromatographic column, and other impurities will flow through. The eluted immunoglobulin solution enters the product production line 25 through the purification and sterilization treatment branch 24. The first anion exchange device 18 and the second anion exchange device 21 differ in that the fillers are different.

[0031] In the embodiment of the utility model, compared with a single process to separate a single active protein, membrane filtration and chromatography technology are combined to separate different active proteins by utilizing the molecular weight, charge property, hydrophobicity, etc. of the protein. That is to say, in this embodiment, the main active proteins in milk can be separated, β-casein, α-lactalbumin, immunoglobulin, and lactoferrin can be separated and purified from the raw milk in sequence, and dried or aseptically filled, and different products can be prepared while retaining the natural nutrients, and the separated milk can still be used to be processed into sterilized milk or milk beverages.

[0032] In an optional embodiment, if Figure 1As shown, the milk protein component synchronous separation system further includes a heat exchanger 5 and a first material temporary storage tank 6 , and the first outlet of the first cation exchange device 4 is connected to the first membrane filtration module 7 through the heat exchanger 5 and the first material temporary storage tank 6 .

[0033] It should be noted that after the lactoferrin-free milk flows through the chromatographic column (flow-through liquid), it is cooled to below 8° C. through the heat exchanger 5 and enters the first material storage tank 6. The storage time in the first material storage tank 6 is 24 hours. After 24 hours of storage, the β-casein in the milk can be dissociated from the casein micelles; wherein, the material in the first material storage tank 6 can be pumped to the first membrane filtration module 7.

[0034] In an optional embodiment, if Figure 1 As shown, the casein solution processing branch includes a second material temporary storage tank 8 , a materializer 9 , a UHT sterilizer 10 and a filler 11 connected in sequence, and the first outlet of the first membrane filtration module 7 is connected to the second material temporary storage tank 8 .

[0035] The UHT sterilizer 10, i.e., ultra-high temperature sterilizer, is a device used for continuous sterilization of milk, juice drinks or similar products. Its working principle is to heat the product to an ultra-high temperature (usually 135-150°C) for several seconds, thereby quickly killing bacteria and achieving a sterilization effect.

[0036] It should be noted that the casein solution enters the second material temporary storage tank 8 for storage, and the casein solution is used for pure milk or milk beverage processing. Specifically, the casein solution passes through the materializer 9, the UHT sterilizer 10 and the filler 11 in sequence, and finally is aseptically canned into milk or milk beverage products.

[0037] In an optional embodiment, if Figure 1 As shown, the milk protein component synchronous separation system also includes a third material temporary storage tank 12, and the second outlet of the first membrane filtration module 7 is connected to the second membrane filtration module 13 through the third material temporary storage tank 12. Among them, the first membrane filtration module 7 can be MF membrane filtration, and the MF membrane pore size is 0.12 microns, and the second membrane filtration module 13 can be UF membrane filtration, and the UF membrane pore size is 20KDa. That is to say, the whey protein solution enters the third material temporary storage tank 12 for storage.

[0038] In addition, the milk protein component synchronous separation system further includes a fourth material temporary storage tank 14, and the second outlet of the second membrane filtration module 13 is connected to the purification and sterilization treatment branch 24 through the fourth material temporary storage tank 14. The β-casein solution enters the fourth material temporary storage tank 14 for storage.

[0039] In an optional embodiment, if Figure 1As shown, the milk protein component synchronous separation system further includes a fifth material temporary storage tank 15 , and the first outlet of the second membrane filtration module 13 is connected to the second cation exchange device 16 via the fifth material temporary storage tank 15 .

[0040] It should be noted that the whey protein solution enters the fifth material temporary storage tank 15 for storage, and then enters the second cation exchange device 16 through a pump and a flow meter. The protein content is detected in real time by an ultraviolet protein sensor.

[0041] In an optional embodiment, if Figure 1 As shown, the synchronous separation system of milk protein components also includes a sixth material temporary storage tank 17 and a seventh material temporary storage tank 19. The first outlet of the second cation exchange device 16 is connected to the first anion exchange device 18 through the sixth material temporary storage tank 17, and the first anion exchange device 18 is connected to the purification and sterilization treatment branch 24 through the seventh material temporary storage tank 19.

[0042] It should be noted that after the whey protein solution enters the second cation exchange device 16, α-lactalbumin and β-lactoglobulin will flow through and be stored in the sixth material temporary storage tank 17. The α-lactalbumin and β-lactoglobulin solution in the sixth material temporary storage tank 17 is pumped to the first anion exchange device 18, wherein the α-lactalbumin will be adsorbed on the chromatographic column, while the β-lactoglobulin will flow through. The eluted α-lactalbumin solution is stored in the seventh material temporary storage tank 19.

[0043] In an optional embodiment, if Figure 1 As shown, the synchronous separation system of milk protein components also includes an eighth material temporary storage tank 20 and a ninth material temporary storage tank 22. The second outlet of the second cation exchange device 16 is connected to the second anion exchange device 21 through the eighth material temporary storage tank 20, and the second anion exchange device 21 is connected to the purification and sterilization treatment branch 24 through the ninth material temporary storage tank 22.

[0044] It should be noted that after the whey protein solution enters the second cation exchange device 16, the immunoglobulin in the whey protein will be adsorbed on the cation chromatographic column, and the eluted immunoglobulin will be stored in the eighth material storage tank 20. The immunoglobulin solution stored in the eighth material storage tank 20 is pumped to the second anion exchange device 21, wherein the immunoglobulin will be adsorbed on the chromatographic column, while other impurities will flow through, and the eluted immunoglobulin will be stored in the ninth material storage tank 22.

[0045] In an optional embodiment, if Figure 1 As shown, the milk protein component synchronous separation system further includes a tenth material temporary storage tank 23 , and the second outlet of the first cation exchange device 4 is connected to the purification and sterilization treatment branch 24 through the tenth material temporary storage tank 23 .

[0046] It should be noted that the lactoferrin solution is stored in the tenth material temporary storage tank 23 , and the lactoferrin solution in the tenth material temporary storage tank 23 can be pumped to the purification and sterilization treatment branch 24 .

[0047] like Figure 1 As shown, in an optional embodiment, the purification and sterilization processing branch 24 includes a concentration and purification device and a sterilization filter connected in sequence.

[0048] It should be noted that the purification and sterilization treatment branch 24 can remove the salt in each solution and filter out the bacteria in the solution to make the solution commercially sterile.

[0049] In an optional embodiment, if Figure 1 As shown, the product production line 25 includes a freeze drying branch, a spray drying branch and a filling branch connected in parallel.

[0050] It should be noted that the β-casein solution, α-lactalbumin solution, immunoglobulin solution, and lactoferrin solution obtained from top to bottom enter the product production line 25 through the purification and sterilization treatment branch 24. The purification and sterilization treatment branch 24 can remove the salt in each solution and filter out the bacteria in the solution to make the solution commercially sterile. In the product production line 25, a freeze drying device can be selected to freeze into powder, a spray drying device can be selected to spray into powder, or a sterile filling machine can be used to make liquid sterile packaging.

[0051] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the utility model.

Claims

1. A system for synchronous separation of milk protein components, characterized in that: include: Milk silo, fat separator, first cation exchange device, first membrane filtration module, casein solution processing branch, second membrane filtration module, second cation exchange device, first anion exchange device, second anion exchange device, purification and sterilization processing branch and product production line; The milk silo, the fat separator and the first cation exchange device are connected in sequence, the first outlet of the first cation exchange device is connected to the first membrane filtration module, the first outlet of the first membrane filtration module is connected to the casein solution processing branch, the second outlet of the first membrane filtration module is connected to the second membrane filtration module, the first outlet of the second membrane filtration module is connected to the second cation exchange device, the first outlet of the second cation exchange device is connected to the first anion exchange device, and the second outlet of the second cation exchange device is connected to the second anion exchange device; Among them, the second outlet of the first cation exchange device, the second outlet of the second membrane filtration module, the first anion exchange device and the second anion exchange device are all connected to the purification and sterilization treatment branch, and the purification and sterilization treatment branch is connected to the product production line.

2. The system for synchronous separation of milk protein components according to claim 1, characterized in that: The milk protein component synchronous separation system also includes a heat exchanger and a first material temporary storage tank, and the first outlet of the first cation exchange device is connected to the first membrane filtration module through the heat exchanger and the first material temporary storage tank.

3. The system for synchronous separation of milk protein components according to claim 1, characterized in that: The casein solution processing branch comprises a second material temporary storage tank, a material mixer, a UHT sterilizer and a filling machine which are connected in sequence, and the first outlet of the first membrane filtration module is connected to the second material temporary storage tank.

4. The system for synchronous separation of milk protein components according to claim 1, characterized in that: The milk protein component synchronous separation system further comprises a third material temporary storage tank, and the second outlet of the first membrane filtration module is connected to the second membrane filtration module through the third material temporary storage tank; and / or, A fourth material temporary storage tank, wherein the second outlet of the second membrane filtration module is connected to the purification and sterilization treatment branch through the fourth material temporary storage tank.

5. The system for synchronous separation of milk protein components according to claim 1, characterized in that: The milk protein component synchronous separation system also includes a fifth material temporary storage tank, and the first outlet of the second membrane filtration module is connected to the second cation exchange device through the fifth material temporary storage tank.

6. The system for synchronous separation of milk protein components according to claim 1, characterized in that: The synchronous separation system for milk protein components also includes a sixth material temporary storage tank and a seventh material temporary storage tank. The first outlet of the second cation exchange device is connected to the first anion exchange device through the sixth material temporary storage tank, and the first anion exchange device is connected to the purification and sterilization treatment branch through the seventh material temporary storage tank.

7. The system for synchronous separation of milk protein components according to claim 1, characterized in that: The synchronous separation system for milk protein components also includes an eighth material temporary storage tank and a ninth material temporary storage tank. The second outlet of the second cation exchange device is connected to the second anion exchange device through the eighth material temporary storage tank, and the second anion exchange device is connected to the purification and sterilization treatment branch through the ninth material temporary storage tank.

8. The system for synchronous separation of milk protein components according to claim 1, characterized in that: The milk protein component synchronous separation system also includes a tenth material temporary storage tank, and the second outlet of the first cation exchange device is connected to the purification and sterilization treatment branch through the tenth material temporary storage tank.

9. The system for synchronous separation of milk protein components according to any one of claims 1 to 8, characterized in that: The purification and sterilization treatment branch includes a concentration and purification device and a sterilization filter which are connected in sequence.

10. The system for synchronous separation of milk protein components according to any one of claims 1 to 8, characterized in that: The product production line includes a freeze drying branch, a spray drying branch and a filling branch connected in parallel.

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