Polypeptide synthesis equipment
By designing a polypeptide synthesis device that integrates reactors and multi-stage filtration devices, the problem of difficulty in achieving continuous reaction and filtration operations in existing equipment is solved, and the processing efficiency and product quality are improved.
Patent Information
- Application Number
- CN202421102237.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-20
- Publication Date
- 2025-03-07
- Estimated Expiration
- 2034-05-20
AI Technical Summary
Most of the existing polypeptide synthesis reaction equipment are single-function reaction devices or filtration devices, and it is difficult to achieve continuous operation of reaction and filtration, resulting in low processing efficiency of polypeptide synthesis.
A polypeptide synthesis device is designed, including a reactor, a first filtration device and a second filtration device. By integrating the polypeptide synthesis reaction and filtration operation, the continuous operation of reaction and filtration is achieved, and the quality of the product solution is improved through two-stage filtration.
Continuous operation of peptide synthesis reaction and filtration is achieved, processing efficiency and production efficiency are improved, and higher quality product solutions are obtained through two-stage filtration.
Smart Images
Figure CN222567720U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of chemical equipment, in particular to a polypeptide synthesis device. Background Art
[0002] At present, most of the peptide synthesis reaction equipment on the market are single-function reaction devices or filtration devices, which make it difficult to achieve continuous operation of reaction and filtration, resulting in low processing efficiency of peptide synthesis. Utility Model Content
[0003] Therefore, the technical problem to be solved by the utility model is to overcome the defect that most of the existing peptide synthesis reaction equipment are single-function reaction devices or filtering devices, which make it difficult to achieve continuous operation of reaction and filtration, resulting in low processing efficiency of peptide synthesis.
[0004] In order to overcome the above-mentioned defects, the utility model provides an improved polypeptide synthesis device, comprising:
[0005] A reaction kettle having a closed cavity, wherein the reaction kettle is suitable for carrying out a polypeptide synthesis reaction;
[0006] A first filtering device, connected to the outlet at the bottom of the reactor, and suitable for filtering the polypeptide synthesis reaction product once;
[0007] The second filtering device is connected to the outlet of the first filtering device, and the second filtering device is suitable for secondary filtering of the polypeptide synthesis reaction product; the filtering accuracy of the second filtering device is higher than that of the first filtering device.
[0008] Optionally, a sampling port is provided on the reactor, and the sampling port is suitable for taking out polypeptide synthesis reaction samples.
[0009] Optionally, a transparent observation window is provided on the reactor to observe the polypeptide synthesis reaction status in the reactor.
[0010] Optionally, the reactor is connected to a stirring device, and the stirring device is suitable for stirring the reactants in the reactor.
[0011] Optionally, the stirring device is a magnetic stirring device.
[0012] Optionally, it also includes:
[0013] A pH sensor, wherein the probe of the pH sensor is suitable for extending into the reactor to monitor the pH value of the polypeptide synthesis reaction in the reactor in real time, so as to monitor the progress of the polypeptide synthesis reaction in real time.
[0014] Optionally, it also includes:
[0015] A temperature sensor, wherein the probe of the temperature sensor is suitable for extending into the reactor to monitor the temperature of the polypeptide synthesis reaction in the reactor in real time.
[0016] Optionally, it also includes:
[0017] The gas source is connected to the gas inlet provided at the top of the reactor through the third filtering device. The gas source is suitable for introducing gas into the reactor after the reaction is completed to perform a filter press operation.
[0018] Optionally, the gas is nitrogen.
[0019] Optionally, the reaction kettle, the first filtering device and the second filtering device are all made of stainless steel.
[0020] The above technical solution of the utility model has the following advantages compared with the prior art:
[0021] 1. The polypeptide synthesis equipment provided by the utility model comprises: a reaction kettle having a closed cavity, in which a polypeptide synthesis reaction is suitable for being performed; a first filtering device connected to an outlet provided at the bottom of the reaction kettle, the first filtering device being suitable for performing a primary filtration on a polypeptide synthesis reaction product; a second filtering device connected to the outlet of the first filtering device, the second filtering device being suitable for performing a secondary filtration on a polypeptide synthesis reaction product; the filtering accuracy of the second filtering device is higher than that of the first filtering device; the application adopts the above technical scheme, by combining polypeptide synthesis reaction and filtration on the same polypeptide synthesis equipment, to achieve continuous operation of polypeptide synthesis reaction and filtration, thereby improving processing efficiency and production efficiency; and after two levels of filtration with different accuracies, a product solution with better quality is obtained.
[0022] 2. The reactor, the first filter device and the second filter device described in the utility model are all made of stainless steel; this application adopts the above technical solution. By adopting stainless steel, the reactor, the first filter device and the second filter device have good corrosion resistance and high temperature resistance, thereby extending the service life of the polypeptide synthesis equipment.
[0023] 3. The reactor of the utility model is connected with a stirring device, which is suitable for stirring the reactants in the reactor; the present application adopts the above technical solution to promote the mixing and reaction of the reactants through the stirring device.
[0024] 4. The polypeptide synthesis equipment provided by the utility model also includes: a pH sensor, the probe of the pH sensor is suitable for extending into the reactor to monitor the pH value of the polypeptide synthesis reaction in the reactor in real time, so as to monitor the progress of the polypeptide synthesis reaction in real time; the present application adopts the above technical solution to conveniently monitor the progress of the reaction through the pH sensor. BRIEF DESCRIPTION OF THE DRAWINGS
[0025] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0026] Figure 1 This is a schematic diagram of the connection structure of the polypeptide synthesis device provided in the embodiment of the utility model.
[0027] Description of reference numerals:
[0028] 1. Reactor; 2. First filter device; 3. Second filter device; 4. Third filter device; 5. Gas source; 6. pH sensor; 7. Temperature sensor; 8. Sampling port; 9. Observation window; 10. Stirring device; 11. Reaction zone; 12. Filtration zone; 13. Water inlet; 14. Water outlet; 15. Opening. DETAILED DESCRIPTION
[0029] The technical solution of the utility model will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0030] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.
[0031] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0032] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.
[0033] like Figure 1 A specific embodiment of the peptide synthesis device shown includes: a reactor 1, a first filter device 2, a second filter device 3, a pH sensor 6, a temperature sensor 7 and a gas source 5. Specifically, the reactor 1 is a reaction tank, and the volume of the reaction tank can be 100 liters; the reactor 1, the first filter device 2 and the second filter device 3 are all made of stainless steel; the first filter device 2 and the second filter device 3 are both liquid filters.
[0034] The reactor 1 has a closed cavity, and is suitable for carrying out a polypeptide synthesis reaction in the reactor 1. The first filter device 2 is connected to an outlet provided at the bottom of the reactor 1, and the first filter device 2 is suitable for filtering the polypeptide synthesis reaction product once. The second filter device 3 is connected to the outlet of the first filter device 2, and the second filter device 3 is suitable for filtering the polypeptide synthesis reaction product twice; the filtering accuracy of the second filter device 3 is higher than the filtering accuracy of the first filter device 2. The first filter device 2 and the second filter device 3 select filter elements of different pore sizes and materials as needed to filter particles of different sizes to obtain a product solution. The filter element pore size of the second filter device 3 is smaller than the filter element pore size of the first filter device 2.
[0035] The probe of the pH sensor 6 is suitable for extending into the reactor 1 to monitor the pH value of the polypeptide synthesis reaction in the reactor 1 in real time, so as to monitor the progress of the polypeptide synthesis reaction in real time. The probe of the temperature sensor 7 is suitable for extending into the reactor 1 to monitor the temperature of the polypeptide synthesis reaction in the reactor 1 in real time. The gas source 5 is connected to the gas inlet provided at the top of the reactor 1 through the third filter device 4. The gas source 5 is suitable for introducing gas into the reactor 1 after the reaction is completed to perform a filter press operation; specifically, the gas is nitrogen, and the third filter device 4 is a nitrogen filter. The reactor 1 constitutes a reaction zone 11, such as Figure 1 The first filtering device 2 and the second filtering device 3 together constitute a filtering area 12, such as Figure 1 As shown in the dashed box on the right.
[0036] Furthermore, the reactor 1 is provided with a sampling port 8, from which a polypeptide synthesis reaction sample is suitable for taking out. The reactor 1 is provided with a transparent observation window 9 to observe the polypeptide synthesis reaction status in the reactor 1. Specifically, the observation window 9 is a strip sight glass. The bottom of the reactor 1 is connected with a stirring device 10, and the stirring device 10 is suitable for stirring the reactants in the reactor 1. Specifically, the stirring device 10 is a magnetic stirring device. The reactor 1 is provided with a water inlet 13 and a water outlet 14, and the water inlet 13 is suitable for introducing hot water or chilled water into the reactor 1, and the water outlet is suitable for discharging the hot water or chilled water in the reactor 1. An opening 15 is provided on the top of the reactor 1, and the opening 15 is suitable as a hand hole or a feeding hole.
[0037] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived from these are still within the scope of protection of the invention of the utility model.
Claims
1. A polypeptide synthesis device, characterized in that: include: A reaction kettle (1) having a closed cavity, wherein the reaction kettle (1) is suitable for carrying out a polypeptide synthesis reaction; A first filtering device (2) is connected to an outlet provided at the bottom of the reaction kettle (1), and the first filtering device (2) is suitable for filtering the product of the polypeptide synthesis reaction once; The second filtering device (3) is connected to the outlet of the first filtering device (2), and the second filtering device (3) is suitable for secondary filtering of the polypeptide synthesis reaction product; the filtering accuracy of the second filtering device (3) is higher than the filtering accuracy of the first filtering device (2).
2. The polypeptide synthesis device according to claim 1, characterized in that: The reaction kettle (1) is provided with a sampling port (8), and the sampling port (8) is suitable for taking out polypeptide synthesis reaction samples.
3. The polypeptide synthesis device according to claim 1, characterized in that: The reactor (1) is provided with a transparent observation window (9) for observing the polypeptide synthesis reaction status in the reactor (1).
4. The peptide synthesis device according to any one of claims 1 to 3, characterized in that: The reactor (1) is connected to a stirring device (10), and the stirring device (10) is suitable for stirring the reactants in the reactor (1).
5. The polypeptide synthesis device according to claim 4, characterized in that: The stirring device (10) is a magnetic stirring device.
6. The peptide synthesis device according to any one of claims 1 to 3, characterized in that: Also includes: A pH sensor (6), wherein a probe of the pH sensor (6) is adapted to be inserted into the reaction kettle (1) to monitor the pH value of the polypeptide synthesis reaction in the reaction kettle (1) in real time, so as to monitor the progress of the polypeptide synthesis reaction in real time.
7. The peptide synthesis device according to any one of claims 1 to 3, characterized in that: Also includes: A temperature sensor (7), wherein a probe of the temperature sensor (7) is adapted to extend into the reactor (1) to monitor the temperature of the polypeptide synthesis reaction in the reactor (1) in real time.
8. The peptide synthesis device according to any one of claims 1 to 3, characterized in that: Also includes: The gas source (5) is connected to the gas inlet provided at the top of the reactor (1) through the third filtering device (4). The gas source (5) is suitable for introducing gas into the reactor (1) after the reaction is completed to perform a filter press operation.
9. The polypeptide synthesis device according to claim 8, characterized in that: The gas is nitrogen.
10. The peptide synthesis device according to any one of claims 1 to 3, characterized in that: The reaction kettle (1), the first filtering device (2) and the second filtering device (3) are all made of stainless steel.