Glass slide and microbiological detection equipment
By setting a hydrophilic coating layer and hydrophobic area on the slide, the problems of suspension spillage and incomplete automatic scanning in traditional slides are solved, achieving higher detection accuracy and sample protection.
Patent Information
- Application Number
- CN202322490921.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2023-09-13
- Publication Date
- 2025-03-07
- Estimated Expiration
- 2033-09-13
AI Technical Summary
During the automatic digital scanning and detection of traditional slides, the suspension to be tested is prone to spillover, resulting in sample loss, and lacks specific smear areas, so it is impossible to perform automatic microscope scanning, and all cells cannot be classified and counted.
A glass slide was designed including a transparent substrate, a detection area and a hydrophobic area. The detection area is covered with a hydrophilic coating layer to prevent the detection liquid from overflowing; the hydrophobic area is arranged along the edge of the detection area to prevent the liquid from overflowing and cause the overflowing liquid to return to the detection area.
Effectively prevent the overflow of the suspension to be tested, reduce sample losses, ensure the accuracy of the automatic scanning of the microscope, and be able to classify and count all cells, improving the accuracy of the data.
Smart Images
Figure CN222579926U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of glass slides, in particular to glass slides and microorganism detection equipment. Background Art
[0002] With the development of in vitro diagnostic laboratory construction plans, various methodological testing projects have been automated, but the operation and result preservation of traditional morphological diagnostic technology have developed slowly and are still operated manually, especially for: blood, cerebrospinal fluid, bone marrow, prostatic fluid, duodenal drainage fluid, sputum, semen, leucorrhea, urine, feces and other human body fluids, bone marrow, exudates, transudates, secretions and excretions of cells, casts, bacteria, microorganisms and other microorganisms and tissue samples. Different slides or observation media are used for preparation, and unified morphological automatic digital scanning cannot be completed.
[0003] In the process of realizing the present invention, the inventor of the present invention found that: at present, during the automatic digital scanning and detection process, when the suspension to be tested is dripped onto the existing glass slide, the volume of the suspension to be tested generally cannot exceed 50 μL, otherwise the suspension to be tested will overflow on the glass slide, causing the loss of the sample to be tested, especially in the process of covering the glass slide with a cover glass, the loss of microorganisms is more serious. At the same time, the existing glass slide does not have a specific smear area, and cannot be scanned automatically by a microscope, and cannot classify and count all cells, and can only obtain cell information of a part of the area under the microscope field of view. Summary of the invention
[0004] In view of the above problems, the present utility model is proposed to provide a glass slide and a microorganism detection device that overcome the above problems or at least partially solve the above problems.
[0005] In a first aspect, an embodiment of the utility model provides a glass slide, comprising: a transparent substrate;
[0006] A detection area is provided on a side of the transparent substrate, and the detection area has at least one hydrophilic coating layer;
[0007] Furthermore, the hydrophilic coating layer completely covers the detection area to prevent the detection liquid from overflowing.
[0008] Furthermore, the glass slide further comprises a hydrophobic region, the hydrophobic region is arranged on the transparent substrate, and the hydrophobic region and the detection region are arranged on the same side of the transparent substrate;
[0009] The hydrophobic area is provided with a hydrophobic material layer, and the hydrophobic material layer is arranged along the edge of the detection area.
[0010] Furthermore, the thickness of the hydrophilic coating layer is less than or equal to that of the hydrophobic material layer.
[0011] Furthermore, the thickness of the hydrophobic material layer is set to gradually decrease from its edge away from the hydrophilic coating layer toward the hydrophilic coating layer.
[0012] Furthermore, the thickness of the hydrophilic coating layer is controlled to be between 5 μm and 20 μm.
[0013] Furthermore, the hydrophilic coating layer is made of fluorine surfactant, dodecanol polyoxyethylene ether, octanol polyoxyethylene ether, quaternary ammonium salt or hexadecyl trimethyl ammonium bromide.
[0014] Furthermore, the shape of the hydrophilic coating layer includes circular, rectangular, elliptical or polygonal.
[0015] Furthermore, the hydrophobic material layer includes a coating layer made of at least one material selected from the group consisting of fluorocarbon coating, polyolefin, polycarbonate, polyamide, polyacrylonitrile and polyester.
[0016] Furthermore, the thickness of the hydrophobic material layer is controlled to be between 5 μm and 20 μm.
[0017] In a second aspect, an embodiment of the utility model provides a microorganism detection device, which includes any one of the following slides.
[0018] The beneficial effects of the above technical solution provided by the embodiment of the utility model at least include:
[0019] In the embodiment of the utility model, a glass slide is provided, by setting a detection area on a transparent substrate, and setting a hydrophilic coating on the detection area, the hydrophilic coating effectively ensures that the liquid to be detected can be as much as possible in the set detection area, and prevents the liquid to be detected from overflowing. At the same time, the embodiment is also set on the same side of the transparent substrate as the detection area, and the hydrophobic area effectively prevents the liquid to be detected from overflowing, and at the same time, the overflowed liquid can also be returned to the detection area.
[0020] This embodiment can effectively ensure that the volume of the suspension to be tested exceeds the specified amount, and the suspension to be tested will not overflow on the slide, ensuring that there is no loss of the sample to be tested, especially reducing the loss of microorganisms in the process of covering the cover glass on the slide. It is more effective to ensure that the microscope is automatically scanned, all cells are classified and counted, and the cell information of the partial area under the microscope field of view is obtained to ensure the accuracy of the data.
[0021] Other features and advantages of the utility model will be described in the following description, and partly become apparent from the description, or understood by implementing the utility model. The purpose and other advantages of the utility model can be realized and obtained by the structures particularly pointed out in the written description and the drawings.
[0022] The technical solution of the utility model is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] In order to more clearly illustrate the specific implementation of the utility model or the technical solution in the prior art, the following is a brief introduction to the drawings required for the specific implementation or the prior art description. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, each element or part is not necessarily drawn according to the actual scale.
[0024] Figure 1 It is a schematic diagram of the first embodiment of the glass slide of the utility model;
[0025] Figure 2 This utility model Figure 1 Another perspective of the picture;
[0026] Figure 3 This utility model Figure 2 AA section view;
[0027] Figure 4 It is an AA cross-sectional view of another embodiment of the glass slide of the utility model;
[0028] Figure 5 This utility model Figure 4 B is a partial enlarged view;
[0029] Figure 6 This is an AA cross-sectional view of another embodiment of the glass slide of the utility model;
[0030] Figure 7 This utility model Figure 6 B is a partial enlarged view;
[0031] Figure 8 It is a schematic diagram of a second embodiment of the glass slide of the utility model;
[0032] Fig. 9 It is a schematic diagram of the third embodiment of the glass slide of the utility model;.
[0033] Indicated in the figure:
[0034] 10. Transparent substrate;
[0035] 20. Hydrophilic coating layer;
[0036] 30. Hydrophobic material layer. DETAILED DESCRIPTION
[0037] The following embodiments of the technical solution of the utility model are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the utility model, and are therefore only used as examples, and cannot be used to limit the protection scope of the utility model.
[0038] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application should have the common meanings understood by technicians in the field to which the utility model belongs.
[0039] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.
[0040] In addition, the terms "first", "second", etc. are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. In the description of the present utility model, the meaning of "plurality" is more than two, unless otherwise clearly and specifically limited.
[0041] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this utility model can be understood according to specific circumstances.
[0042] In the present application, unless otherwise clearly specified and limited, a first feature being “above” or “below” a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being “above”, “above”, and “above” a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being “below”, “below”, and “below” a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.
[0043] The utility model provides a glass slide in an embodiment, which is used to hold a liquid object for inspection under a microscope. Usually, the liquid to be detected is mounted on the glass slide, and then the two are inserted into a microscope for observation. This glass slide is used in fields such as microscopic observation and experimental research in biology, medicine, chemistry, geology, etc. If the glass slide can prevent overflow, it can well ensure that the detection target in the liquid to be detected is not dispersed, and a more accurate target can be observed, so that the detection result is closer to the actual situation.
[0044] Reference Figure 1 As shown, a glass slide comprises: a transparent substrate 10;
[0045] A detection area is provided on a side of the transparent substrate 10, and the detection area has at least one hydrophilic coating layer 20;
[0046] Furthermore, the hydrophilic coating layer 20 completely covers the detection area to prevent the detection liquid from overflowing.
[0047] It can be understood that by setting a detection area on the transparent substrate 10 and setting a hydrophilic coating layer 20 on the detection area, the hydrophilic coating layer 20 can effectively ensure that the liquid to be detected can be as much as possible in the set detection area through its affinity for liquid, and prevent the liquid to be detected from overflowing. And the hydrophilic coating layer 20 can be one or more. For example, in some embodiments, the hydrophilic coating layer 20 is one, and the hydrophilic coating layer 20 completely covers the detection area; in other embodiments, refer to Figure 8 As shown, the hydrophilic coating layer 20 is two, and the two hydrophilic coating layers 20 arranged at intervals completely cover the detection area; in other embodiments, referring to Fig. 9 As shown, there are three hydrophilic coating layers 20, and the three hydrophilic coating layers 20 arranged at intervals completely cover the detection area.
[0048] In the embodiments, it should be noted that a glass slide is a glass or quartz sheet used to place objects when observing objects under a microscope. When making samples, cells or tissue slices are placed on the glass slide, and a cover glass is placed on it for observation. In optics, a thin sheet of glass-like material is used to produce phase difference. The detection area here refers to the area on the glass slide where the liquid to be detected is applied, and the position on the glass slide is also observed during detection.
[0049] In actual use, the liquid to be detected is dropped on the detection area. At this time, the detection area has a better affinity with the liquid due to the hydrophilic coating, that is, the liquid can be ensured to be on the detection area to a certain extent. At the same time, by setting a hydrophobic area, the liquid can be effectively prevented from overflowing.
[0050] This embodiment can effectively ensure that the volume of the suspension to be tested exceeds the specified amount, and the suspension to be tested will not overflow on the slide, ensuring that there is no loss of the sample to be tested, especially reducing the loss of microorganisms in the process of covering the cover glass on the slide. It is more effective to ensure that the microscope is automatically scanned, all cells are classified and counted, and the cell information of the partial area under the microscope field of view is obtained to ensure the accuracy of the data.
[0051] Reference Figure 1 As shown, in a further embodiment, the glass slide further includes a hydrophobic region, the hydrophobic region is disposed on the transparent substrate 10, and the hydrophobic region and the detection region are disposed on the same side of the transparent substrate 10;
[0052] The hydrophobic area is provided with a hydrophobic material layer 30 , and the hydrophobic material layer 30 is arranged along the edge of the detection area.
[0053] The embodiment is also arranged on the same side of the transparent substrate 10 as the detection area, and the hydrophobic area effectively prevents the detection liquid from overflowing, and at the same time, the overflowing liquid can also return to the detection area.
[0054] It can be understood that by setting a hydrophilic coating layer 20 on the detection area, the hydrophilic coating layer 20 can effectively maintain the liquid on the detection area to prevent the liquid from overflowing, thereby ensuring the accuracy of the detection. Furthermore, a hydrophobic material layer 30 is also set on the periphery of the detection area. Since the hydrophobic material layer 30 here has hydrophobic properties, it can effectively return the liquid overflowing from the detection area to the detection area, effectively ensuring the accuracy of the data of the detected liquid. The hydrophobic material layer 30 here is set along the edge of the detection area, that is, the hydrophilic coating layer 20 is in contact with the hydrophobic material layer 30, so the design can effectively prevent the liquid from overflowing.
[0055] Reference Figure 3 As shown, in a further embodiment, the thickness of the hydrophilic coating layer 20 is less than or equal to the hydrophobic material layer 30 .
[0056] It is understandable that the thickness of the hydrophobic material layer 30 is set to be greater than the thickness of the hydrophilic coating layer 20, so that liquid can be further prevented from overflowing structurally and the accuracy of the detection data can be ensured. Of course, the thickness of the hydrophobic material layer 30 can also be set to be equal to the thickness of the hydrophilic coating layer 20.
[0057] In actual use, since the thickness of the hydrophobic material layer 30 is greater than the thickness of the hydrophilic coating layer 20, the specific thickness of the hydrophobic material layer 30 is greater than the hydrophilic coating layer 20 by 1 μm to 8 μm, and the specific thickness difference is changed according to actual requirements. For example, in some embodiments, the thickness of the hydrophobic material layer 30 is greater than the hydrophilic coating layer 20 by 1 μm; in other embodiments, the thickness of the hydrophobic material layer 30 is greater than the hydrophilic coating layer 20 by 5 μm; in still other embodiments, the thickness of the hydrophobic material layer 30 is greater than the hydrophilic coating layer 20 by 7 μm. That is, the height difference between the two is formed by the different thicknesses, and the height can effectively prevent the liquid on the hydrophilic coating layer 20 from overflowing to the hydrophobic material layer 30.
[0058] Reference Figure 4 and 5 As shown, in a further embodiment, the thickness of the hydrophobic material layer 30 is set to gradually decrease from its edge away from the hydrophilic coating layer 20 toward the hydrophilic coating layer 20 .
[0059] Due to the current structure of the glass slide, when liquid overflows, the overflowed liquid cannot be returned to the detection area in time, thereby affecting the accuracy of the detection data. It can be understood that in the embodiment, the thickness of the hydrophobic material layer 30 can also be set to a gradually decreasing structure. By setting the hydrophobic material layer 30 to a structure that gradually decreases toward the hydrophilic coating layer 20, that is, the surface is in the form of a slope, it can be effectively ensured that the liquid is returned to the detection area as much as possible under gravity. Specifically, as Figure 4 The thickness of the hydrophobic material layer 30 is set to gradually decrease along the X direction toward the hydrophilic coating layer 20 .
[0060] Specifically, refer to Figure 6 and 7 As shown, the thickness of the hydrophobic material layer 30 is the largest at a position away from the hydrophilic coating layer 20, and the thickness at this position is greater than the thickness of the hydrophilic coating layer 20; the thickness of the hydrophobic material layer 30 is the smallest at a position close to the hydrophilic coating layer 20, and the thickness at this position is greater than or equal to the thickness of the hydrophilic coating layer 20. Furthermore, the thickness of the hydrophobic material layer 30 is gradually reduced from a position away from the hydrophilic coating layer 20 to a position close to the hydrophilic coating layer 20. During use, even if liquid accidentally overflows onto the hydrophobic material layer 30, the liquid can be returned to the hydrophilic coating layer 20 through the slope structure, ensuring the accuracy of the liquid amount detected, thereby also ensuring the accuracy of the detection data.
[0061] In a further embodiment, the hydrophilic coating layer 20 is made of at least one of a fluorine surfactant, polyoxyethylene lauryl alcohol ether, polyoxyethylene octanol ether, a quaternary ammonium salt or hexadecyl trimethyl ammonium bromide.
[0062] It should be noted that the hydrophilic coating layer 20 can be made of different forms of hydrophilic materials. When it is needed, the material needs to be observed under a microscope. That is, it has a hydrophilic function without affecting normal microscope observation, effectively ensuring the basic function of the slide and solving the problem of easy liquid overflow.
[0063] Reference Figure 2 , 8 As shown in Figure 9, in a further embodiment, the shape of the hydrophilic coating layer includes circular, rectangular, elliptical or polygonal.
[0064] It can be understood that the embodiments can set the detection area to different patterns, which are specifically set according to actual requirements. For example, in some embodiments, the detection area is set to be circular, and then a hydrophilic material that completely covers the detection area is set on the circular detection area, that is, a circular hydrophilic coating layer 20 is provided; in other embodiments, the detection area is set to be rectangular, and then a hydrophilic material that completely covers the detection area is set on the rectangular detection area, that is, a rectangular hydrophilic coating layer 20 is provided; in still other embodiments, the detection area is set to be elliptical, and then a hydrophilic material that completely covers the detection area is set on the elliptical detection area, that is, an elliptical hydrophilic coating layer 20 is provided. The actual detection area is set to various patterns according to the detection area required for detection, which increases the fun of detection.
[0065] In a further embodiment, the hydrophobic material layer 30 includes a coating layer made of at least one material selected from the group consisting of fluorocarbon coating, polyolefin, polycarbonate, polyamide, polyacrylonitrile and polyester.
[0066] In the embodiment, the thickness of the hydrophilic coating layer 20 is controlled within the range of 5 μm to 20 μm; the thickness of the hydrophobic material layer 30 is controlled within the range of 5 μm to 20 μm.
[0067] In some embodiments, the thickness of the hydrophilic coating layer 20 is controlled at 1-8 μm, and the thickness of the hydrophobic material layer 30 is controlled at 15 μm; in other embodiments, the thickness of the hydrophilic coating layer 20 is controlled at 10 μm, the thickness of the hydrophobic material layer 30 at a position away from the hydrophilic coating layer 20 is 18 μm, and the thickness of the hydrophobic material layer 30 at a position close to the hydrophilic coating layer 20 is 9 μm, and the thickness of the hydrophobic material layer 30 is gradually reduced from a position away from the hydrophilic coating layer 20 to a position close to the hydrophilic coating layer 20; in still other embodiments, the thickness of the hydrophilic coating layer 20 is controlled at 5 μm, and the thickness of the hydrophobic material layer 30 is controlled at 5 μm.
[0068] Based on the same inventive concept, an embodiment of the utility model further provides a microorganism detection device, which includes any one of the glass slides described below.
[0069] This embodiment can effectively ensure that the volume of the suspension to be tested exceeds the specified amount, and the suspension to be tested will not overflow on the slide, ensuring that there is no loss of the sample to be tested, especially reducing the loss of microorganisms in the process of covering the cover glass on the slide. It is more effective to ensure that the microscope is automatically scanned, all cells are classified and counted, and the cell information of the partial area under the microscope field of view is obtained to ensure the accuracy of the data.
[0070] For specific examples and descriptions of beneficial effects of the method described in this embodiment, reference can be made to the description of the above-mentioned heat dissipation device, which will not be repeated here.
[0071] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. The present disclosure is not limited to the precise structure described above and shown in the accompanying drawings, and various modifications and changes may be made without departing from its scope. The scope of the present disclosure is limited only by the appended claims. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.
Claims
1. A slide glass, characterized in that: include: Transparent substrate; A detection area is provided on a side of the transparent substrate, and the detection area has at least one hydrophilic coating layer; Furthermore, the hydrophilic coating layer completely covers the detection area to prevent the detection liquid from overflowing.
2. The slide glass according to claim 1, characterized in that The glass slide further comprises a hydrophobic region, the hydrophobic region is arranged on the transparent substrate, and the hydrophobic region and the detection region are arranged on the same side of the transparent substrate; The hydrophobic area is provided with a hydrophobic material layer, and the hydrophobic material layer is arranged along the edge of the detection area.
3. The slide glass according to claim 2, characterized in that: The thickness of the hydrophilic coating layer is less than or equal to the thickness of the hydrophobic material layer.
4. The slide glass according to claim 3, characterized in that: The thickness of the hydrophobic material layer is set to gradually decrease from its edge away from the hydrophilic coating layer toward the hydrophilic coating layer.
5. The slide glass according to claim 2, characterized in that: The thickness of the hydrophilic coating layer is controlled to be between 5 μm and 20 μm.
6. The slide glass according to claim 1 or 2, characterized in that: The shape of the hydrophilic coating layer includes circular, rectangular, elliptical or polygonal.
7. The slide glass according to claim 2, characterized in that: The thickness of the hydrophobic material layer is controlled to be between 5 μm and 20 μm.
8. A microbial detection device, characterized in that: The microorganism detection device comprises the glass slide described in any one of claims 1-7.