Cell imaging device
By designing a cell imaging device containing mobile and lifting devices, the cell contamination and resource consumption problems caused by frequent removal of cell culture flasks during cell culture are solved, and real-time and comprehensive monitoring of cell growth status is achieved.
Patent Information
- Application Number
- CN202420877267.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-25
- Publication Date
- 2025-03-14
- Estimated Expiration
- 2034-04-25
AI Technical Summary
During cell culture, frequent removal of the cell culture flask from the incubator and placed on a microscope for observation increases the risk of cell contamination, and frequent opening and closing of the incubator door will increase CO2 consumption and heat loss in the incubator body.
A cell imaging device is designed, which includes a base, a mobile device and a lifting device. The microscope adjusts the distance from the cell culture flask through the lifting device, and moves along the length and width of the cell culture flask through the mobile device to realize real-time monitoring of cells at each position in the cell culture flask.
By reducing the frequent removal of cell culture flasks, the risk of cell contamination is reduced, and CO2 consumption and heat loss caused by frequent opening and closing of the incubator is reduced, real-time and comprehensive monitoring of cell growth status is achieved.
Smart Images
Figure CN222618513U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of cell imaging, and particularly relates to a cell imaging device. Background Art
[0002] After cell culture, its growth, morphology and even biological characteristics need to be continuously observed. Since cells are small and complex, it is difficult to timely understand the cell growth status, quantity changes, cell morphology, cell movement, contamination, etc. without the help of appropriate tools. At present, during the cell culture process, the cell culture flask is usually taken out of the incubator and placed on a microscope for cell observation. However, frequently taking the cell culture flask out of the incubator increases the risk of cell contamination, and the frequent opening and closing of the incubator door will also increase the consumption of CO2 and the loss of heat in the incubator. Utility Model Content
[0003] The utility model provides a cell imaging device to solve the above problems.
[0004] The technical solution of the utility model is achieved in this way:
[0005] A cell imaging device comprises a base, wherein a cavity is arranged inside the base, the top of the base is used to place a cell culture bottle, a moving device is installed inside the base, a lifting device is installed on the moving device, a microscope is installed on the lifting device, the lifting device is used to drive the microscope to move up and down, and is used to adjust the distance between the lens of the microscope and the bottom of the cell culture bottle, and the moving device is used to drive the microscope to move back and forth along the length and width of the cell culture bottle.
[0006] Through the above technical solution, the distance between the lens of the microscope and the bottom of the cell culture bottle can be adjusted by the lifting device, so that the microscope can capture clear images of the cells in the cell culture bottle, and the moving device can drive the microscope to move horizontally and vertically, thereby driving the microscope to move back and forth along the length and width of the cell culture bottle, so that the microscope can capture and monitor the cells at various positions in the cell culture bottle, thereby monitoring the growth status of the cells in the cell culture bottle in real time, avoiding cell contamination caused by frequently taking out the cell culture bottle from the incubator to observe the cell growth status, and reducing the CO2 consumption caused by frequent opening and closing of the incubator door and the heat loss in the incubator body.
[0007] Optionally, a light board B is installed on the top of the base, and the top of the light board B is used to place a cell culture bottle. Two telescopic rods arranged in parallel and spaced apart are installed at one end of the top of the base. The tops of the two telescopic rods are fixedly connected with a top plate, and a light board A is installed on the bottom of the top plate. The light board A corresponds to the light board B up and down and is arranged in parallel and spaced apart.
[0008] Through the above technical solution, the setting of light board A and light board B can improve the clarity of the microscope shooting environment and improve the accuracy of the microscope shooting the cell growth status in the cell culture bottle.
[0009] Optionally, the moving device includes two guide rails A and a guide rail B, the two guide rails A are fixedly connected to the inside of the base, a moving plate A is slidably connected between the two guide rails A, the top of the moving plate A is fixedly connected to the guide rail B, the top of the guide rail B is slidably connected to the moving plate B, and a lifting device is installed on the top of the moving plate B.
[0010] Through the above technical solution, the setting of two guide rails A and one guide rail B can realize the movement of the microscope in two directions, so that the movement of the microscope covers all monitoring positions on the bottom of the cell culture bottle, and improves the comprehensiveness and accuracy of the microscope movement monitoring the cell growth status in the cell culture bottle.
[0011] Optionally, a motor A and a mounting frame A are installed inside the base, and the motor A and the mounting frame A are respectively located at the two ends of one of the guide rails A, and pulleys A are installed on the output shaft of the motor A and the side of the mounting frame A, a synchronous belt A is sleeved and connected between the two pulleys A, and the synchronous belt A is fixedly connected to the movable plate A, and a motor B and a mounting frame B are installed on the top of the movable plate A, and the motor B and the mounting frame B are respectively located at the two ends of the guide rail B, and pulleys B are installed on the output shaft of the motor B and the side of the mounting frame B, a synchronous belt B is sleeved and connected between the two pulleys B, and the synchronous belt B is fixedly connected to the movable plate B.
[0012] Through the above technical scheme, the cooperation of motor A, mounting frame A, pulley A and synchronous belt A can change the position of movable plate A, and movable plate A moves along the length direction of guide rail A, so that movable plate A can drive the microscope to move along the length direction of cell culture bottle. The cooperation of motor B, mounting frame B, pulley B and synchronous belt B can change the position of movable plate B, and movable plate B moves along the length direction of guide rail B, so that movable plate B can drive the microscope to move along the width direction of cell culture bottle, thereby realizing automatic control of the moving direction of the microscope and realizing all-round cell growth monitoring on the bottom of cell culture bottle.
[0013] Optionally, the synchronous belt A is fixedly connected to the movable plate A via a fixed plate A, and the synchronous belt B is fixedly connected to the movable plate B via a fixed plate B.
[0014] Through the above technical solution, the fixed plate A can improve the firmness and stability of the connection and fixation between the synchronous belt A and the movable plate A, and the fixed plate B can improve the firmness and stability of the connection and fixation between the synchronous belt B and the movable plate B.
[0015] Optionally, the lifting device includes a lifting frame, a vertical plate and a cylinder, the cylinder and the vertical plate are installed on the top of the movable plate B, the side of the vertical plate is slidably connected with the lifting frame, the lifting frame is located between the vertical plate and the cylinder, the microscope is installed inside the lifting frame, the lens of the microscope is set upward, and the piston rod of the cylinder is vertically upward and fixedly connected to the lifting frame.
[0016] Through the above technical solution, the cylinder drives the lifting frame to move up and down to adjust the height of the microscope, thereby adjusting the distance between the lens of the microscope and the bottom of the cell culture bottle, improving the accuracy of microscope photography, and the vertical plate is slidably connected to the lifting frame, which can improve the stability of the lifting frame during the up and down movement.
[0017] After adopting the above technical solution, the beneficial effects of the utility model are:
[0018] The cell imaging device in the utility model can be placed in an incubator to monitor the cell growth status in real time, reduce the risk of cell contamination caused by frequent taking out of cell culture bottles for observation, and also reduce the amount of electricity and gas (carbon dioxide) used in the laboratory. The staff can complete the observation work during the cell culture process on the office computer without entering the laboratory, reducing the material consumption entering the laboratory. The lens can be moved to any point within the observation area to meet the needs of multi-site observation. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.
[0020] Figure 1 is a schematic diagram of the structure of an imaging device and a cell culture bottle in an embodiment;
[0021] Figure 2 The structure of the imaging device in the embodiment is shown in FIG. Figure Ⅰ ;
[0022] Figure 3 Schematic diagram of the imaging device structure in the embodiment Figure II ;
[0023] Figure 4 The structure of the base in the embodiment is shown in FIG. Figure Ⅰ ;
[0024] Figure 5 The structure of the base in the embodiment is shown in FIG. Figure II ;
[0025] Figure 6 The structure of the base in the embodiment is shown in FIG. Figure III .
[0026] Explanation of the accompanying drawings: 1. base; 2. telescopic rod; 3. top plate; 4. light board A; 5. light board B; 6. cell culture bottle; 7. microscope; 8. guide rail A; 9. guide rail B; 10. moving plate A; 11. moving plate B; 12. motor A; 13. mounting frame A; 14. pulley A; 15. synchronous belt A; 16. fixed plate A; 17. motor B; 18. mounting frame B; 19. pulley B; 20. synchronous belt B; 21. fixed plate B; 22. lifting frame; 23. vertical plate; 24. cylinder. DETAILED DESCRIPTION
[0027] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0028] The embodiment of the present application discloses a cell imaging device.
[0029] Example
[0030] according to Figures 1 to 6 As shown, a cell imaging device includes a base 1, a cavity is arranged inside the base 1, the top of the base 1 is used to place a cell culture bottle 6, a moving device is installed inside the base 1, the moving device is installed with a lifting device, the lifting device is installed with a microscope 7, the lifting device is used to drive the microscope 7 to move up and down, and is used to adjust the distance between the lens of the microscope 7 and the bottom of the cell culture bottle 6, and the moving device is used to drive the microscope 7 to move back and forth along the length and width of the cell culture bottle 6.
[0031] A light board B5 is installed on the top of the base 1, and a cell culture bottle 6 is placed on the top of the light board B5. Two telescopic rods 2 are installed at one end of the top of the base 1 and are arranged in parallel and spaced apart. A top plate 3 is fixedly connected to the top of the telescopic rod 2, and a light board A4 is installed at the bottom of the top plate 3. The light board A4 is arranged in parallel and spaced apart from the light board B5.
[0032] The moving device includes two guide rails A8, a guide rail B9, a moving plate A10 and a moving plate B11. Two parallel and spaced guide rails A8 are installed inside the base 1. The moving plate A10 is slidably connected between the two guide rails A8. The top of the moving plate A10 is fixedly connected with the guide rail B9. The guide rail B9 is vertically arranged with the guide rail A8. The top of the guide rail B9 is slidably connected with the moving plate B11.
[0033] The lifting device includes a cylinder 24, a vertical plate 23 and a lifting frame 22. The cylinder 24 and the vertical plate 23 are installed at intervals in parallel on the top of the movable plate B11. The piston rod of the cylinder 24 is vertically upward. The side of the vertical plate 23 is connected to the lifting frame 22 for sliding up and down. The lifting frame 22 is located between the vertical plate 23 and the cylinder 24. A microscope 7 is installed inside the lifting frame 22. The lens of the microscope 7 is set upward. The end of the piston rod of the cylinder 24 is fixedly connected to the lifting frame 22. The piston rod of the cylinder 24 drives the lifting frame 22 to move up and down by telescoping.
[0034] A motor A12 and a mounting frame A13 are installed inside the base 1, and a motor B17 and a mounting frame B18 are installed on the top of the movable plate A10. The motor A12 and the mounting frame A13 are respectively located at the two ends of one of the guide rails A8, and the motor B17 and the mounting frame B18 are respectively located at the two ends of the guide rail B9. The output shaft of the motor A12 and the side of the mounting frame A13 are both installed with pulleys A14, and a synchronous belt A15 is sleeved and connected between the two pulleys A14. The synchronous belt A15 is fixedly connected to the movable plate A10 through a fixed plate A16. The motor A12 drives the synchronous belt A15 to rotate, thereby driving the movable plate A10 to move back and forth along the length direction of the guide rail A8, thereby driving the microscope 7 to move back and forth along the length direction of the guide rail A8. Pulleys B19 are installed on the output shaft of motor B17 and the side of mounting frame B18, and a synchronous belt B20 is sleeved and connected between the two pulleys B19. The synchronous belt B20 is fixedly connected to the movable plate B11 through the fixed plate B21. Motor B17 drives the synchronous belt B20 to rotate, thereby driving the movable plate B11 to move back and forth along the length direction of the guide rail B9, thereby driving the microscope 7 to move back and forth along the length direction of the guide rail B9.
[0035] The microscope 7 can be electrically connected to the controller, and the microscope 7 sends the captured pictures to the controller, so that the operator can remotely obtain information about the cell culture conditions. The controller belongs to the prior art, and the method of connecting it to the microscope 7 to obtain picture information also belongs to the prior art, which will not be described in detail here.
[0036] Motor A12, motor B17 and cylinder 24 can also be electrically connected to the controller, which is responsible for controlling the start and stop of motor A12, motor B17 and cylinder 24, thereby performing remote operation. This also belongs to the prior art and will not be elaborated here.
[0037] Working principle:
[0038] Place the cell culture bottle 6 on the light board B5 at the top of the base 1, turn on the light boards A4 and B5, start the microscope 7, and start the cylinder 24 according to the shooting distance between the lens of the microscope 7 and the cell culture bottle 6. The piston rod of the cylinder 24 adjusts the up and down movement of the lifting frame 22 by telescoping, and the lifting frame 22 drives the microscope 7 to move up and down, so as to facilitate the maintenance of a suitable shooting distance between the lens of the microscope 7 and the bottom of the cell culture bottle, thereby improving the accuracy and convenience of photographing cells by the microscope 7. Put the entire device and the cell culture bottle 6 into the incubator for cell culture. After the cells have been cultured for a period of time, use the microscope 7 to observe and monitor the growth status of the cells.
[0039] Start the motor A12, and the motor A12 drives the pulley A14 to rotate. The pulley A14 drives the synchronous belt A15 to rotate by rotating. During the rotation process, the synchronous belt A15 drives the movable plate A10 to move along the length direction of the guide rail A8, thereby driving the entire guide rail B9 and the movable plate B11 to move synchronously along the length direction of the guide rail A8, and then drives the microscope 7 to move synchronously, so that the microscope 7 moves along the length direction of the cell culture bottle 6. At this time, the lens of the microscope 7 will shoot the cell culture conditions of the cell culture bottle 6 along the length direction. Then start the motor B17, which drives the pulley B19 to rotate. The pulley B19 drives the synchronous belt B20 to rotate by rotating. During the rotation, the synchronous belt B20 drives the movable plate B11 to move along the length direction of the guide rail B9, thereby driving the microscope 7 to move synchronously, so that the microscope 7 moves along the width direction of the cell culture bottle 6. Start the motor A12 again, and the motor A12 drives the lens of the microscope 7 to move along the length direction of the cell culture bottle 6 again. Repeat the above operation, so that the lens of the microscope 7 can capture every position on the bottom of the cell culture bottle 6, thereby obtaining the cell culture status in the entire cell culture bottle 6.
[0040] In the description of the present utility model, it should be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In the description of the present utility model, unless otherwise specified and limited, it should be noted that the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it can be a mechanical connection or an electrical connection, or it can be the internal communication of two elements, it can be a direct connection, or it can be an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to specific circumstances.
[0041] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A cell imaging device, characterized in that: It comprises a base, wherein a cavity is arranged inside the base, the top of the base is used to place a cell culture bottle, a moving device is installed inside the base, a lifting device is installed on the moving device, a microscope is installed on the lifting device, the lifting device is used to drive the microscope to move up and down, and is used to adjust the distance between the lens of the microscope and the bottom of the cell culture bottle, and the moving device is used to drive the microscope to move back and forth along the length and width of the cell culture bottle.
2. A cell imaging device according to claim 1, characterized in that: A light board B is installed on the top of the base, and the top of the light board B is used to place a cell culture bottle. Two telescopic rods arranged in parallel and spaced apart are installed at one end of the top of the base. The tops of the two telescopic rods are fixedly connected with a top plate, and a light board A is installed on the bottom of the top plate. The light board A corresponds to the light board B up and down and is arranged in parallel and spaced apart.
3. A cell imaging device according to claim 1, characterized in that: The moving device includes two guide rails A and a guide rail B. The two guide rails A are fixedly connected to the inside of the base. A moving plate A is slidably connected between the two guide rails A. The top of the moving plate A is fixedly connected to the guide rail B. The top of the guide rail B is slidably connected to the moving plate B. A lifting device is installed on the top of the moving plate B.
4. A cell imaging device according to claim 3, characterized in that: A motor A and a mounting frame A are installed inside the base, and the motor A and the mounting frame A are respectively located at the two ends of one of the guide rails A, and pulleys A are installed on the output shaft of the motor A and the side of the mounting frame A, and a synchronous belt A is sleeved and connected between the two pulleys A, and the synchronous belt A is fixedly connected to the movable plate A. A motor B and a mounting frame B are installed on the top of the movable plate A, and the motor B and the mounting frame B are respectively located at the two ends of the guide rail B, and pulleys B are installed on the output shaft of the motor B and the side of the mounting frame B, and a synchronous belt B is sleeved and connected between the two pulleys B, and the synchronous belt B is fixedly connected to the movable plate B.
5. A cell imaging device according to claim 4, characterized in that: The synchronous belt A is fixedly connected to the movable plate A via a fixed plate A, and the synchronous belt B is fixedly connected to the movable plate B via a fixed plate B.
6. A cell imaging device according to claim 3, characterized in that: The lifting device includes a lifting frame, a vertical plate and a cylinder. The cylinder and the vertical plate are installed on the top of the movable plate B. The lifting frame is slidably connected to the side of the vertical plate. The lifting frame is located between the vertical plate and the cylinder. The microscope is installed inside the lifting frame. The lens of the microscope is set upward. The piston rod of the cylinder is vertically upward and fixedly connected to the lifting frame.
Citation Information
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