Culture dish for detection of inhibition zone
By designing an annular baffle on the inner bottom of the main body of the dish for antibacterial circle detection, the experimental deviation caused by inconsistent amount of solid culture medium in the traditional antibacterial circle detection method is solved, and antibacterial circle detection with simple operation and accurate results is achieved.
Patent Information
- Application Number
- CN202421132975.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-23
- Publication Date
- 2025-03-18
- Estimated Expiration
- 2034-05-23
AI Technical Summary
In traditional antibacterial circle detection methods, inconsistent addition of solid culture medium will lead to different diameters of antibacterial circles, resulting in experimental deviations, and cumbersome operations, which can easily lead to solidification and waste.
A petri dish for antibacterial circle detection was designed. There is an annular baffle on the inside of the bottom of the main body of the dish, allowing solid culture medium to be poured directly into the baffle to ensure the consistency of volume and reduce deviation.
Through this design, the tedious operation of measuring cylinders or weighing weight is avoided, the volume consistency of the solid culture medium is ensured, the deviation of antibacterial circle measurement is reduced, and the reproducibility and accuracy of the experiment is improved.
Smart Images
Figure CN222631437U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to a culture dish for detecting a bacteriostasis zone, in particular to a culture dish which can detect the bacteriostasis zone more accurately and conveniently, and belongs to the field of glass and plastic products. Background Art
[0002] In the field of microbiology research and medicine, the inhibition zone test is a commonly used method to evaluate the inhibitory ability of antibiotics on bacteria. In the traditional inhibition zone test method, a proper amount of indicator bacteria is added to the solid test medium. Generally, 15~20mL is poured into an ordinary 90mm culture dish. After solidification, the antibiotic is added to the test liquid using an Oxford cup, filter paper or hole digging method, and then cultured for a period of time. If antibiotics can inhibit the growth of bacteria, a sterile area, i.e., an inhibition zone, will be formed on the culture dish. The diameter of the inhibition zone can be used to judge the antibacterial effect. Because the antibacterial substance diffuses from the starting position to the surrounding of the culture medium, the thicker the culture medium, the more obvious the concentration difference between the surface and the bottom of the culture medium, resulting in different diameters of the inhibition zone formed on the surface and the bottom, which has a certain impact on the measurement work of the experimenter and will form experimental deviations. However, when routinely performing antibacterial tests, multiple plates are required to repeat parallel experiments to ensure that the amount of solid test medium added between the plates is consistent, the thickness of the solid culture medium is the same, and the distribution of antibacterial substances in the culture medium can maintain a consistent diffusion state. The experimental results are reproducible, which is crucial to the accuracy and precision of the inhibition zone data. Usually, measuring with a measuring cylinder or weighing and packaging with a balance is used to ensure consistency. This method is cumbersome to operate. First, it often causes solid culture medium to solidify during processing, making it unusable and causing waste. Second, like the measuring cylinder method, the accuracy is still not high, resulting in deviations in the inhibition zone. Utility Model Content
[0003] The utility model aims to provide a culture dish for detecting inhibition zone, which can ensure the consistency of the volume of solid culture medium in different culture dishes when preparing solid culture medium for detecting inhibition zone, without complicated operations such as measuring with a measuring cylinder or weighing the weight, and avoid the deviation effect on the determination of inhibition zone caused by different addition amounts of culture medium.
[0004] The technical scheme of the utility model is: a culture dish for detecting antibacterial zone, comprising a culture dish cover and a culture dish body, wherein an annular baffle is arranged inside the bottom of the culture dish body of the culture dish.
[0005] The annular baffle plate on the inner side of the bottom of the culture dish body of the culture dish is lower than the outer wall of the body and forms a concentric circle structure with the outer wall.
[0006] The annular baffle on the inner side of the bottom of the culture dish body of the culture dish has a gap with the outer wall.
[0007] The gap between the annular baffle and the outer wall is preferably 2 to 10 mm. BRIEF DESCRIPTION OF THE DRAWINGS
[0008] The utility model is further described below in conjunction with the accompanying drawings.
[0009] Figure 1 Culture medium front view
[0010] Figure 2 Top view of the culture dish
[0011] In the figure, 1. culture dish cover, 2. culture dish body, 3. annular baffle, 4. outer wall. DETAILED DESCRIPTION
[0012] from Figure 1 It can be seen that a culture dish for detecting inhibition zones is composed of a culture dish cover (1), a culture dish body (2), and an annular baffle (3) at the inner side of the bottom of the culture dish body (2).
[0013] The annular baffle on the inner side of the bottom of the culture dish body is lower than the outer wall of the body and is concentric with the outer wall layer with a certain gap.
[0014] When using this culture dish for inhibition zone detection to prepare solid detection culture medium, pour the solid culture medium directly into the space inside the internal annular baffle until it is slightly full. The slightly excess culture medium will overflow into the outer cavity for storage, which can ensure that the content and height of the culture medium in the annular baffle are consistent. Performing inhibition zone experiments on it can reduce deviations.
Claims
1. A culture dish for detecting inhibition zone, comprising a culture dish cover (1) and a culture dish body (2), characterized in that: An annular baffle (3) is provided on the inner side of the bottom of the culture dish body (2); the height of the annular baffle (3) is lower than the height of the outer wall (4), and forms a concentric circle structure with the outer wall (4); and there is a gap between the annular baffle (3) and the outer wall (4).
2. A culture dish for detecting inhibition zone according to claim 1, characterized in that: The gap distance between the annular baffle (3) and the outer wall (4) is 2 to 10 mm.