Culture apparatus

Abstract

The present application provides a culture apparatus. The culture equipment comprises a shell, the shell provides a closed culture space, and the direction from one end of the shell to the other end of the shell is defined as a first direction; in the culture space, along the first direction, a first assembly comprising a light source and a gas supply assembly used for providing gas required by the culture space, and a second assembly comprising a light source and a gas supply assembly used for providing gas required by the culture space are sequentially arranged; the optical observation assembly comprises a shell and a function part, and the function part is arranged in the shell; the light path structure provided by the culture equipment comprises that a light beam is emitted from the light source, penetrates through the culture sample and the light-transmitting area of the shell and is received by the functional part so as to form and output an observation image of the culture sample.

Description

Technical Field

[0001] The present application relates to a cultivation device. Background Art

[0002] Culture equipment, such as incubators, are box devices mainly used to culture microorganisms, plant and animal cells. They simulate the growth environment of microorganisms, tissues, cells, etc., provide stable temperature, humidity and gas concentration, and are widely used in cell, tissue culture and the reproduction and cultivation of certain special microorganisms.

[0003] During the culture process, there is a need to detect the growth status of the culture sample. However, the incubator is generally a closed space. If the incubator is frequently opened and samples are taken for testing during the culture process, it will have a negative impact on the culture process.

[0004] In this regard, in a comparative scheme, the inventors tried to set up a commonly used microscope for observing biological samples in a closed space. However, due to its large size and the fact that it is not suitable for being placed in an incubator with a high temperature and high humidity environment, it is impossible to perform long-term observation of the cultured samples while culturing in a closed incubator. Utility Model Content

[0005] The purpose of the present application is to realize that during the culture process in a closed culture device, the culture device can be kept closed during the culture process and the state of the culture sample can be observed during the culture cycle.

[0006] A culture device according to the first aspect of the present application comprises: a shell, the shell provides a closed culture space, and the direction from one end to the other end of the shell is defined as a first direction; in the culture space, arranged in sequence along the first direction are: a first component, including a light source and a gas supply component, the gas supply component is used to provide the gas required by the culture space; an optical observation component, including an outer shell and a functional part, the functional part is arranged inside the outer shell; the optical path structure provided by the culture device comprises: a light beam is emitted from the light source, penetrates the culture sample, the light-transmitting area of ​​the outer shell, and is received by the functional part to form and output an observation image of the culture sample.

[0007] In one or more embodiments of the culture device, the light source and the gas supply component of the first component are arranged at one end of the closed culture space.

[0008] In one or more embodiments of the culture device, the boundary of the projection of the light source on the optical observation component along the first direction exceeds or coincides with the boundary of the light-transmitting area of ​​the optical observation component in the length direction and / or width direction.

[0009] In one or more embodiments of the cultivation device, the light source comprises a plurality of light sources, and the plurality of light sources are arranged at intervals in the length direction and / or the width direction.

[0010] In one or more embodiments of the culture device, the functional part includes an optical component, a moving mechanism and an imaging mechanism, and the optical path structure includes: a light beam is emitted from the light source, penetrates the culture sample, penetrates the light-transmitting area, passes through the optical component, and reaches the imaging mechanism; wherein the optical component is connected to the moving mechanism, so that the optical component is a moving part, and the optical component can be driven by the moving mechanism, so that the optical component and the light-transmitting area can move relative to each other.

[0011] In one or more embodiments of the culture device, the optical component includes an objective lens and one or more prisms, and the optical path structure includes: a light beam is emitted from the light source, penetrates the culture sample, penetrates the light-transmitting area, is converged by the objective lens, and reaches the imaging chip of the imaging mechanism after being deflected by the prism.

[0012] In one or more embodiments of the culture device, the optical component includes a plurality of prisms, including at least a first prism and a second prism; the optical path structure includes: the light beam is converged by the objective lens, deflected 90° by the first prism, and then deflected 90° by the second prism to reach the imaging chip of the imaging mechanism.

[0013] In one or more embodiments of the culture device, the moving mechanism includes a first direction driving mechanism, a second direction driving mechanism, and a third direction driving mechanism, the first direction, the second direction, and the third direction are perpendicular to each other, and the second direction and the third direction constitute a plane parallel to the light-transmitting area; wherein the first direction driving mechanism, the second direction driving mechanism, and the third direction driving mechanism include linear motors, and the optical component is driven by the linear motors to move relative to the light-transmitting area in the first direction, the second direction, and the third direction.

[0014] In one or more embodiments of the culture device, the light-transmitting area, the second direction driving mechanism, and the third direction driving mechanism are integrated on a plate-like member, which provides an end cover of the outer shell. The plate-like member is provided with a light-transmitting member to provide a light-transmitting area. The back side of the plate-like member is provided with a guide rail, a grating scale or a magnetic grating scale, an encoder, and a magnetic rod matching the linear motor. The linear motor includes a magnetic axis linear motor supported by the magnetic rod.

[0015] In one or more embodiments of the culture device, the optical component and the imaging mechanism constitute a packaging module, and the packaging module is connected to the first direction driving mechanism, the second direction driving mechanism, and the third direction driving mechanism.

[0016] According to a second aspect of the present application, a culture method using the culture device described in the first aspect comprises the following steps:

[0017] S1. The culture sample is placed in the culture space of the culture device and is sealed and cultured during the culture period;

[0018] S2. During the culture cycle, the optical observation component photographs the culture sample during a set observation time period to obtain and output the observation image; wherein, when photographing, the light source of the first component is turned on, and the gas supply component stops running; after the photographing is completed, the gas supply component continues to run.

[0019] In one or more embodiments of the culturing method, in S2, the observation time period is 1s to 10s, preferably, the observation time period can also be 5s to 10s, 8s to 10s, 1s to 5s, or 1s to 3s.

[0020] In one or more embodiments of the culture method, in S2, multiple observation cycles are set, and the observation cycle includes at least one observation time period. When there are multiple observation time periods in the same observation cycle, the optical observation component photographs different positions of the culture sample corresponding to different observation time periods. The frequency and time of the observation cycle can be set according to actual experimental requirements. For example, the duration of the observation cycle does not exceed 5 min / h at most, preferably, the duration of the observation cycle does not exceed 3 min / h at most, and more preferably, the duration of the observation cycle does not exceed 2 min / h at most.

[0021] In one or more embodiments of the culture device, it includes: a memory for storing instructions executable by a processor; a processor for executing the instructions to implement the steps of the culture method of the second aspect that can be implemented by a computer program.

[0022] According to a third aspect of the present application, a computer-readable storage medium has a computer program thereon, and the program is executed by a processor to implement the steps in the culture method described in the second aspect that can be implemented by the computer program.

[0023] A computer program product according to the fourth aspect of the present application includes a computer program, which, when executed by a processor, implements the steps in the cultivation method described in the second aspect that can be implemented by the computer program. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The above and other features, properties and advantages of the present application will become more apparent through the following description in conjunction with the accompanying drawings and embodiments, in which:

[0025] Figure 1A , Figure 1B Schematic diagram of the structure of a culture device according to an embodiment.

[0026] Figure 2 for Figure 1B AA direction schematic diagram.

[0027] Figure 3 It is a schematic diagram of the internal structure of the optical observation component of the culture device according to one embodiment.

[0028] Figure 4 It is a schematic diagram of the structure of the functional parts of the optical observation component of the culture device according to one embodiment.

[0029] Figure 5 It is a schematic diagram of a culture method according to an embodiment. DETAILED DESCRIPTION

[0030] The following discloses a variety of different implementation methods or embodiments of the subject technical solution. To simplify the disclosure, specific examples of each element and arrangement are described below. Of course, these are only examples and are not intended to limit the scope of protection of the present invention. For example, the first feature subsequently recorded in the specification is formed above or on the second feature, which may include an implementation method in which the first and second features are formed by direct connection, and may also include an implementation method in which an additional feature is formed between the first and second features, so that the first and second features may not be directly connected. In addition, the figure marks and / or letters may be repeated in different examples in these disclosures. This repetition is for simplicity and clarity, and does not itself represent the relationship between the various implementation methods and / or structures to be discussed. Further, when the first element is described in a manner connected or combined with the second element, the description includes an implementation method in which the first and second elements are directly connected or combined with each other, and also includes the use of one or more other intervening elements to indirectly connect or combine the first and second elements.

[0031] It is to be understood that the following uses a flow chart to illustrate the steps performed by the culture method according to the embodiments of the present application. It should be understood that, according to actual circumstances, the preceding or following steps may not necessarily be performed accurately in order. Other steps may also be added to these processes, or a step or several steps may be removed from these processes.

[0032] refer to Figure 1A , Figures 1B to 4 As shown, the culture device 100 includes a housing 1 , a first component 2 , and an optical observation component 3 .

[0033] The housing 1 provides a closed culture space 10, and the direction from one end to the other end of the housing 1 is defined as the first direction. Figure 1A as well as Figure 1B As shown, the vertical direction from the top to the bottom of the housing 1 is defined as the first direction. The structure of the housing 1, such as the box structure shown in the figure, can be a cube / cuboid structure formed by welding multiple plates, and the material can be stainless steel, but it is not limited to this.

[0034] In the culture space 10, the first component 2 and the optical observation component 3 are arranged in sequence from the top to the bottom in the vertical direction. The first component 2 includes a light source 21 and a gas supply component 22. The gas supply component 22 is used to provide the gas required by the culture space 10, for example, CO 2 , O 2 、N 2 The specific structure of the gas supply component 22 can be an air duct structure arranged on the top of the culture device 100, and the air duct is used to promote the air circulation in the culture space to improve the uniformity of the gas in the culture space. A fan and a high-efficiency filter can also be arranged in the air duct, and the high-efficiency filter can filter and clean the gas. The light source 21 can emit a light beam to provide imaging in the optical observation component 3.

[0035] Here, the light source 21 and the air supply component 22 are classified as components of the first component 2, which means that the light source 21 and the air supply component 22 are integrated. Figure 1B as well as Figure 2 As shown, the light source 21 and the air supply component 22 of the first component 2 are arranged on the entire or part of the top of the closed culture space, for example Figure 2 As shown, the first component 2 occupies part of the space of the top, but it is not limited thereto and may also occupy the entire space. The light source 21 is disposed on the surface of the top, and the air duct of the air supply component 22 is disposed inside the top.

[0036] The optical observation assembly 3 includes a housing 31 and a functional part 32, and the functional part 32 is arranged inside the housing 31. The housing 31 has a light-transmitting area 311 at the top, so that the light beam emitted by the light source 21 can be received by the functional part 32. The specific structure of the housing 31 can be, for example, Figure 1A , Figure 1B As shown, it is composed of a lower box body 312, an upper cover body 313, and a light-transmitting area 311 (transparent stage) integrated in the upper cover body 313. The outer shell 31, except for the light-transmitting area 311, can be made of stainless steel, aluminum alloy, etc., so that the components inside the observation system will not be affected by the humidity and temperature in the culture space, and can work stably in an environment of high temperature, high humidity and a certain pressure for a long time. For example, the lower box body 312 can be stainless steel, the upper cover body 313 can be aluminum alloy, and the material of the light-transmitting area 311 can be super-clean glass, but it is not limited to this.

[0037] In other embodiments, ventilation holes may be provided on the housing 31 for heat dissipation of the functional part 32. In order to prevent the optical observation assembly 3 from being affected by the humidity in the culture space, when the housing 31 is provided with ventilation holes, the internal components may be sealed with a sealant. In a preferred embodiment, for example Figure 4 As shown, when ventilation holes are provided on the housing 31, a fan 33 may also be provided inside the housing to assist in heat dissipation, thereby ensuring a constant temperature inside the culture space.

[0038] Preferably, in some embodiments, Figure 1B As shown, the structure of the light-transmitting area 311 and the light source 21 can be that the boundary of the projection of the light source 21 along the first direction (i.e., the vertical direction) on the optical observation component exceeds or coincides with the boundary of the light-transmitting area 311 of the optical observation component in the length direction and / or the width direction, for example Figure 1B As shown, the boundary of the projection of the light source 21 on the optical observation component along the first direction (i.e., the vertical direction) basically coincides with or slightly exceeds the boundary of the upper cover body 313 in the length direction, so it must coincide with or exceed the boundary of the light-transmitting area 311 provided on the upper cover body 313 in the length direction or the width direction. Such a beneficial effect is that the inventors have found that, compared with the common practice in the art of setting the light source 21 at the center position corresponding to the light-transmitting area 311 to form an intermediate divergent light path structure, the boundary of the light source 21 is extended to exceed or coincide with the boundary of the light-transmitting area 311 of the optical observation component in the length direction and / or the width direction, which significantly improves the imaging quality, especially for the structure of a compact culture device 100 that is in a sealed culture space and has an integrated air supply component 22 around it. The structure of the light source 21 shown in the figure, compared with the central divergent light path structure, the quality of the observed image obtained is higher, thereby ensuring the reliability of the observation result. Preferably, as Figure 2 As shown, in some embodiments, the number of light sources 21 can be multiple, for example Figure 2 As shown, the two or more light sources are arranged at intervals in the length direction and / or the width direction. Compared with a single large light source, this can save the cost of setting the light source while not affecting the gas circulation path of the culture device itself. The illumination of the light source can be adjusted from 0 to 100000. In addition, the distance between the light source 21 and the light-transmitting area 311 can also be adjusted, for example, by Figure 1B The adjustment mechanism 211 shown adjusts the distance between the two in the vertical direction, so that it can adapt to different forms of culture samples, for example, it can observe culture dishes, multi-well plates, and multi-layer cell factories. It can be understood that the form of the culture sample can also be a standard T25 cell culture bottle, a T75 cell culture bottle, a T300 cell culture bottle, a multi-well culture plate, etc., which is not limited to this.

[0039] The functional unit 32 is, as the name implies, a mechanism capable of forming and outputting an observation image of the culture sample.

[0040] In this way, the optical path structure provided by the culture device 100 includes: a light beam is emitted from the light source 21, penetrates the culture sample, the light-transmitting area 311 of the housing 31, and is received by the functional part 32 to form and output an observation image of the culture sample. It can be understood that the observation image here is not limited to a static image, but can also be a dynamic image of a short time (e.g., 1s to 3s).

[0041] In some embodiments, reference Figure 3 as well as Figure 4 As shown, the structure of the functional part 32 may include an optical component 321, a moving mechanism 322 and an imaging mechanism 323, and the optical path structure described above includes: a light beam is emitted from the light source 21, penetrates the culture sample, penetrates the light-transmitting area 311, passes through the optical component 321, and reaches the imaging mechanism 323; wherein the optical component 321 is connected to the moving mechanism 322, so that the optical component 321 is a moving part, and the optical component 321 can be driven by the moving mechanism 322, so that the optical component 321 and the light-transmitting area 311 can move relative to each other, so that the optical component 321 can be adjusted to the ideal position for desired observation and imaging, and observation and imaging of the culture sample at multiple positions and multiple angles can also be achieved. In some embodiments, continue to refer to Figure 4 As shown, the optical component 321 may include an objective lens 3211 and one or more prisms 3212, and the corresponding optical path structure may be: a light beam is emitted from the light source 21, penetrates the culture sample, penetrates the light-transmitting area 311, converges through the objective lens 3211, and reaches the imaging chip of the imaging mechanism 323 after being deflected by the prism 3212. The meaning of "objective lens" here is similar to the objective lens in a microscope, that is, a lens group composed of a plurality of lenses. The purpose of combined use is to overcome the imaging defects of a single lens and improve the optical quality of the objective lens. The magnifying effect of a microscope mainly depends on the objective lens. The meaning of "prism" is also similar to that in the art, that is, a transparent object surrounded by two planes that intersect but are not parallel to each other, used to split light or disperse the light beam. The combination of objective lens + prism can further improve the quality of imaging. Preferably, for multiple prisms, for example, including a first prism 32121 and a second prism 32122; the optical path structure includes: the light beam is converged by the objective lens, deflected 90° by the first prism, and then deflected 90° by the second prism to reach the imaging chip of the imaging mechanism, which can further improve the imaging quality.

[0042] Continue to refer Figure 3 as well as Figure 4In some embodiments, the structure of the moving mechanism 322 may include a first direction driving mechanism 3221, a second direction driving mechanism 3222, and a third direction driving mechanism 3223. The first direction, the second direction, and the third direction are perpendicular to each other. The second direction and the third direction constitute a plane parallel to the light-transmitting area. For example, the first direction shown in the figure is the vertical direction, that is, the Z direction, and the second direction and the third direction are length and width directions, respectively, that is, the X and Y directions shown in the figure.

[0043] Among them, the first direction driving mechanism 3221, the second direction driving mechanism 3222, and the third direction driving mechanism 3223 include a linear motor 3224, and the linear motor 3224 drives the optical component 321 to move relative to the light-transmitting area 311 in the first direction, the second direction, and the third direction, so as to adjust the optical component 321 to the ideal position for the desired observation and imaging, and also realize the observation and imaging of multiple positions and multiple angles of the culture sample. Specifically, the second direction driving mechanism 3222 and the third direction driving mechanism 3223 can drive the optical component 321 to move in the plane, so as to realize the observation of the sample at any position on the light-transmitting area 311 (transparent stage), and the first direction driving mechanism 3221 drives the optical component 321 to move in the vertical direction, so as to make the imaging clearer. The optical component 321 can move in the first direction, the second direction, and the third direction, so as to avoid the influence and disturbance on the cells or microorganisms and their culture solution when adjusting the observation position, and can perform in-situ observation at multiple points in a large area without affecting the growth and movement environment of the cells or microorganisms. Furthermore, the structure of the linear motor 3224 is easy to control and has high position repeatability, so that the observation of the same position at different times can be accurately realized.

[0044] Preferably, in some embodiments, continue to refer to Figure 3 as well as Figure 4 As shown, the arrangement structure of the moving mechanism 322 may be that the light-transmitting area 311, the second direction driving mechanism 3222, and the third direction driving mechanism 3223 are integrated in an upper cover body 313 which is a plate-like member. The upper cover body 313 of the plate-like member provides an end cover of the outer shell 31. The upper cover body 313 is provided with a light-transmitting member to provide the light-transmitting area 311. The back side of the upper cover body 313 is provided with a guide rail 3225, a grating scale 3226, an encoder 3227, and a magnetic rod 3228 matching the linear motor 3224. The linear motor 3224 is a magnetic axis linear motor supported by the magnetic rod 3228. Such an arrangement allows the moving mechanism to be compactly integrated into the upper cover body 313, so that the structure of the optical observation component 3 is compact. In another embodiment, a magnetic scale can be used instead of the grating scale 3226 to improve the linear motor's ability to withstand high humidity. Preferably, as Figure 3 as well as Figure 4As shown, the optical component 321 and the imaging mechanism 323 constitute a packaging module 320, that is, they are packaged by an outer layer of wrapping material, so that the optical component 321 and the imaging mechanism 323 are protected. The packaging module 320 is connected to the first direction driving mechanism 3221, the second direction driving mechanism 3222, and the third direction driving mechanism 3223, so that the integration of the optical observation component 3 is good and the structure is compact, and the optical component 321 and the imaging mechanism 323 are further protected, and the imaging quality is further improved. The packaging mentioned only refers to the integration of the optical component and the imaging mechanism into a space and is packaged by an outer layer of wrapping material, which can include complete sealing of the outer layer of wrapping material, and can also include incomplete sealing with ventilation holes set on the outer layer of wrapping material.

[0045] In summary, the culture device 100 introduced in the above embodiment has the beneficial effect of realizing the process of culture in the closed culture device, so that the culture device can be kept closed during the culture process and the state of the culture sample can be observed during the culture cycle.

[0046] like Figure 5 As shown, as described above, the present application further provides a culture method, using the culture device 100 described in the above embodiment, the culture method comprises the following steps:

[0047] S1. The culture sample is set in the culture space 10 of the culture device 100 and is sealed for culture during the culture period; for example, as recorded above, the culture sample can be placed in a standard T25 cell culture flask, T75 cell culture flask, T300 cell culture flask, multi-well culture plate, cell factory, etc., and is set in the culture space 10. It can be understood that the position of the culture sample is set in the vertical direction between the first component 2 and the optical observation component 3; in one embodiment, the culture sample can be placed directly on the light-transmitting area (transparent stage), and the culture period can be set according to the needs of the experiment.

[0048] S2. During the culture cycle, the optical observation component 3 photographs the culture sample during the set observation time period to obtain and output the observation image; wherein, when photographing, the light source 21 of the first component 2 is turned on, and the gas supply component 22 stops running; after the photographing is completed, the gas supply component 22 continues to run, preferably, the light source 21 is turned off when the gas supply component 22 is running to avoid interfering with the culture process.

[0049] That is, the first component 2 can be electrically connected to the control unit 4 and controlled to realize the control of stopping or running the light source 21 and the gas supply component 22. The control unit 4 generally includes a processor 41 and a memory 42. The memory 42 is used to store instructions that can be executed by the processor 41; the processor 41 is used to execute the instructions to implement the steps executed by the program in the culture method introduced in this application. It should be noted that the above-mentioned memory and processor are not limited to a specific memory and processor. For example, in some cases, both the memory and the processor can have a distributed structure. For example, it can include a memory and a processor located at the culture device end and the background cloud, respectively, and the culture device end and the background cloud jointly implement the above-mentioned culture method. Furthermore, in an embodiment using a distributed structure, each step can adjust the specific execution terminal according to the actual situation, and the specific scheme of each step implemented at a specific terminal should not limit the scope of protection of the present invention.

[0050] The beneficial effect of this is that the imaging quality of the observed image can be significantly improved. The reason is probably that the inventor accidentally discovered in practice that the important factor affecting the imaging quality may be due to the compact structure of integrating the light source 21 and the air supply component 22 in the first component 2. When the observation system takes pictures, the controller controls the fan, air circuit and other equipment of the air supply component 22 that will disturb the sample to be temporarily closed during shooting, so as to ensure the stability of the airflow in the culture space 10 and prevent vibration, thereby improving the imaging quality. After the shooting is completed, the motor power supply of the fan of the air supply component 22 and the power supply of the air circuit solenoid valve are almost immediately restored. Since the shooting time itself is short, it will not affect the culture sample.

[0051] In some embodiments, the time of the above observation time period is 1s to 10s. Preferably, the time of the observation time period can also be 5s to 10s, 8s to 10s, 1s to 5s, 1s to 3s, so that the imaging quality can be guaranteed and the adverse effect on the culture process can be avoided. It can be understood that for multiple observation time periods, different observation time periods can correspond to different times. For example, the time of the observation time period mentioned above can also be 5s to 10s, 8s to 10s, which is generally the first observation time period, and time for adjusting photosensitivity and focusing needs to be reserved. The time of the observation time period after this is generally shorter, such as 1s to 5s, 1s to 3s. Preferably, for the situation where observation images need to be obtained for multiple positions and / or multiple angles of the culture sample, multiple observation cycles can be set, and the observation cycle includes at least one observation time period. When there are multiple observation time periods in the same observation cycle, the optical observation component photographs different positions of the culture sample corresponding to different observation time periods. Specifically, for the experimenter operating the culture device 100, the following operations can be performed. First, the shooting interval time, shooting position and / or shooting angle within the same observation cycle are set on the software interface corresponding to the culture device 100. The specific shooting interval time, shooting position and / or shooting angle can be set according to the experimental requirements. Before shooting, the imaging of the optical observation component 3 can be pre-adjusted to make the imaging clear. For example, the second direction driving mechanism 3222 and the third direction driving mechanism 3223 are energized to generate magnetic force to drive the optical component on the magnetic bar 3228 to move frictionlessly. At the same time, the encoder 3227 will read the real-time position data of the grating ruler 3226 or the magnetic ruler during the movement of the mover and feed it back to the control unit 4, so as to accurately locate the horizontal position. After the optical component 321 reaches the shooting position, the mover of the first direction driving mechanism 3221 is energized to generate magnetic force to drive the optical component 321 to move frictionlessly, and drive the optical component 321 to move in the vertical direction to achieve focusing, so that the imaging is clear. When it is necessary to take a picture, turn on the light source 21, turn off the air supply component 22 to stop it, for example, turn off the fan, gas circuit solenoid valve and / or optical observation component fan in the culture device, complete the picture taking at the first shooting position and / or shooting angle (hereinafter referred to as "shooting point") in the observation cycle, immediately turn off the light source 21 after the picture taking is completed, and restore the air supply component 22 to continue to operate. After that, the second direction driving mechanism 3222 and the third direction driving mechanism 3223 are used to move to the remaining shooting points of the observation cycle in sequence, take pictures point by point, and after completing the first round of point-by-point scanning cycle, standby according to the set interval time, and after the waiting time is over, start the second observation cycle, and then repeat the cycle for long-term automatic observation and shooting, and the observation images are continuously sent to the control mechanism while shooting.For the allocation of standby and shooting time, it can be adjusted according to actual needs, for example, the duration of the observation cycle is no more than 5min / h, that is, in the culture cycle, every 1 hour, the duration of the observation cycle is no more than 5 minutes, preferably, the duration of the observation cycle is no more than 3min / h, more preferably, the duration of the observation cycle is no more than 2min / h. However, it can be understood that the duration of the observation cycle needs to meet the requirement of not affecting the normal culture process, and the upper limit of the specific duration can be adjusted according to different culture objects and culture processes. For example, the upper limit of the duration of the observation cycle corresponding to different culture objects and culture processes can be obtained through experiments. In addition, if it is necessary to stop the automatic cycle, it can be stopped by the stop button on the software interface, or it can be manually operated by the button on the interface of the culture device itself.

[0052] According to another aspect of the present application, the present application also provides a computer-readable medium.

[0053] The computer-readable medium provided in the present application has computer instructions thereon. When the computer instructions are executed by a processor, the program can be executed by the processor to implement the steps executed by the program in the culture method described in the above embodiment.

[0054] According to yet another aspect of the present application, the present application also provides a computer program product.

[0055] The above-mentioned computer-readable medium provided in the present application includes a computer program, and when the computer program is executed by a processor, the program can be executed by the processor to implement the steps executed by the program in the culture method introduced in the above embodiment.

[0056] In summary, the beneficial effects of the culture equipment, culture method, computer-readable storage medium, and computer program product introduced above are that, during the culture process in a closed culture equipment, the culture equipment can observe the status of the culture sample during the culture cycle while maintaining the closure during the culture process.

[0057] The steps of the method described in conjunction with the embodiments disclosed herein may be embodied directly in hardware, in a software module executed by a processor, or in a combination of the two. The software module may reside in a RAM memory, a flash memory, a ROM memory, an EPROM memory, an EEPROM memory, a register, a hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art. An exemplary storage medium is coupled to a processor so that the processor can read and write information from / to the storage medium. In an alternative, a storage medium may be integrated into a processor. The processor and the storage medium may reside in an ASIC. The ASIC may reside in a user terminal. In an alternative, the processor and the storage medium may reside in a user terminal as discrete components.

[0058] In one or more exemplary embodiments, the functions described may be implemented in hardware, software, firmware, or any combination thereof. If implemented as a computer program product in software, each function may be stored on or transmitted by a computer-readable medium as one or more instructions or codes. Computer-readable media include both computer storage media and communication media, including any media that facilitates the transfer of a computer program from one place to another. Storage media may be any available media that can be accessed by a computer. As an example and not limitation, such a computer-readable medium may include RAM, ROM, EEPROM, CD-ROM or other optical disk storage, disk storage or other magnetic storage device, or any other medium that can be used to carry or store the desired program code in the form of an instruction or data structure and can be accessed by a computer. Any connection is also properly referred to as a computer-readable medium. For example, if the software is transmitted from a website, server, or other remote source using a coaxial cable, fiber optic cable, twisted pair, digital subscriber line (DSL), or wireless technologies such as infrared, radio, and microwaves, the coaxial cable, fiber optic cable, twisted pair, DSL, or wireless technologies such as infrared, radio, and microwaves are included in the definition of the medium. Disk and disc as used herein include compact disc (CD), laser disc, optical disc, digital versatile disc (DVD), floppy disk and Blu-ray disc, wherein disk often reproduces data magnetically, while disc reproduces data optically with lasers. Combinations of the above should also be included within the scope of computer-readable media.

[0059] Although the present application is disclosed as above with preferred embodiments, it is not intended to limit the present application, and any person skilled in the art may make possible changes and modifications without departing from the spirit and scope of the present application. Therefore, any modification, equivalent change and modification made to the above embodiments based on the technical essence of the present application without departing from the content of the technical solution of the present application shall fall within the scope of protection defined by the claims of the present application.

Claims

1. A culture device, characterized in that include: A shell, wherein the shell provides a closed culture space, and a direction from one end to the other end of the shell is defined as a first direction; In the culture space, arranged in sequence along the first direction are: A first component includes a light source and a gas supply component, wherein the gas supply component is used to provide the gas required by the culture space; An optical observation assembly comprises a housing and a functional part, wherein the functional part is arranged inside the housing; The optical path structure provided by the culture device includes: a light beam is emitted from the light source, penetrates the culture sample, the light-transmitting area of ​​the shell, and is received by the functional part to form and output an observation image of the culture sample.

2. The culture device according to claim 1, characterized in that The light source and the gas supply component of the first component are arranged at one end of the closed culture space.

3. The culture device according to claim 2, characterized in that: The boundary of the projection of the light source on the optical observation component along the first direction exceeds or overlaps with the boundary of the light-transmitting area of ​​the optical observation component in the length direction and / or width direction.

4. The culture device according to claim 3, characterized in that: The light source includes a plurality of light sources, and the plurality of light sources are arranged at intervals in a length direction and / or a width direction.

5. The culture device according to claim 1, characterized in that: The functional part includes an optical component, a moving mechanism and an imaging mechanism, and the optical path structure includes: a light beam is emitted from the light source, penetrates the culture sample, penetrates the light-transmitting area, passes through the optical component, and reaches the imaging mechanism; wherein the optical component is connected to the moving mechanism, so that the optical component is a moving part, and the optical component can be driven by the moving mechanism, so that the optical component and the light-transmitting area can move relative to each other.

6. The culture device according to claim 5, characterized in that The optical component includes an objective lens and one or more prisms, and the optical path structure includes: a light beam is emitted from the light source, penetrates the culture sample, penetrates the light-transmitting area, converges through the objective lens, and reaches the imaging chip of the imaging mechanism after being deflected by the prism.

7. The culture device according to claim 6, characterized in that: The optical component includes a plurality of prisms, including at least a first prism and a second prism; the optical path structure includes: the light beam is converged by the objective lens, deflected 90° by the first prism, and then deflected 90° by the second prism to reach the imaging chip of the imaging mechanism.

8. The culture device according to claim 5, characterized in that: The moving mechanism includes a first direction driving mechanism, a second direction driving mechanism, and a third direction driving mechanism, the first direction, the second direction, and the third direction are perpendicular to each other, and the second direction and the third direction constitute a plane parallel to the light-transmitting area; wherein the first direction driving mechanism, the second direction driving mechanism, and the third direction driving mechanism include linear motors, and the optical component is driven by the linear motors to move relative to the light-transmitting area in the first direction, the second direction, and the third direction.

9. The culture device according to claim 8, characterized in that The light-transmitting area, the second direction driving mechanism, and the third direction driving mechanism are integrated on a plate-like member, which provides an end cover of the shell. The plate-like member is provided with a light-transmitting member to provide a light-transmitting area. The back side of the plate-like member is provided with a guide rail, a grating scale or a magnetic grating scale, an encoder, and a magnetic bar that match the linear motor. The linear motor includes a magnetic axis linear motor supported by the magnetic bar.

10. The culture device according to claim 8, characterized in that The optical components and the imaging mechanism constitute a packaging module, and the packaging module is connected to the first direction driving mechanism, the second direction driving mechanism, and the third direction driving mechanism.

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