Novel cast-off cell embedding box

Abstract

The utility model discloses a novel cast-off cell embedding box which comprises a base, a cover body is movably arranged on the base, a bulge is arranged on the cover body, a ring opening is formed in the base, the size of the bulge is matched with that of the ring opening, and when the cover body is buckled on the base, the bulge is positioned in the ring opening. A notch is formed in the ring opening. According to the novel cast-off cell embedding box provided by the utility model, a cell cluster is placed through the base, the base is buckled through the cover body, the cell cluster is placed through the ring opening on the base, and the aggregation of cells is ensured through the bulge on the cover body, so that the subsequent cell cluster embedding work is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of cell embedding boxes, in particular to a novel exfoliated cell embedding box. Background Art

[0002] Exfoliative cytopathology is currently a diagnostic tool, enabling pathologists to determine whether cells are benign or malignant. Pathological cytology can be performed on samples such as pleural effusions, ascites, urine, sputum, joint fluid, bronchoscopy, and fine-needle aspiration tissue. While traditional cytology and liquid-based cytology methods are rapid, simple, and economical, they can sometimes present diagnostic challenges due to the small number of cells collected, uneven smear thickness, atypical cell morphology, and high background impurities. This makes it difficult to identify the source of tumor cells and prevents clinicians from providing more effective information.

[0003] According to the disclosure (announcement) number: CN208921521U, the disclosure (announcement) date: 2019-05-31, a embedding box for making cell wax blocks is disclosed, including a box body, a box cover, a wax immersion area, a solidification area, a horizontal partition, a vertical partition, a bushing, and a molding cavity, characterized in that: the box body is rotatably connected to the box cover, and a cavity opening upward is formed inside the box body, and the vertical partition evenly divides the cavity inside the box body into a wax immersion area (the same as the first wax immersion area) and a solidification area. Horizontal partitions are installed in the wax immersion area and the solidification area, and a bushing is fixedly installed on the horizontal partition, and a molding cavity is built into the bushing; the horizontal partition is installed in the middle and upper part of the wax immersion area and the solidification area, and the surrounding area is closed to the inner wall of the box body, and at least 4 bushings are installed on the horizontal partition, which are evenly distributed.

[0004] It is known from the above patents and prior art that the current clinical method for collecting exfoliated cells and making cell wax blocks is to centrifuge the body fluid containing exfoliated cells, discard the supernatant, collect the cell mass at the bottom, wrap it into a square bag with filter paper, put the cell mass into the bag, fold and seal it, and put the wrapped filter paper into a tissue embedding box for subsequent fixation, dehydration, transparency, wax dipping, embedding and other work. However, such operation will have different pathology laboratory technicians. The size of the filter paper bag folded by the pathologist is different. If the filter paper bag is too large and too thick, it is easy to cause the lid of the tissue embedding box to collapse during subsequent operations, and the filter paper with the specimen falls out of the embedding box, causing specimen confusion. The filter paper after the filter paper affects the processing of the cells by the reagent, resulting in unsatisfactory results of subsequent related tests, especially molecular pathology testing. If the filter paper bag is too small, it is easy to cause the cell mass to be missed during tissue processing, and finally lead to the loss of the specimen, which is a great medical safety hazard. Because the filter paper is made of cotton fiber, during the later wax block embedding process, the embedding personnel will use tweezers to scrape the cell clusters on the filter paper into the embedding mold. This can easily bring the cotton fiber into the cell wax block, and scratches are likely to appear in the subsequent preparation process, affecting the doctor's reading of the film. Utility Model Content

[0005] The utility model aims to provide a novel exfoliated cell embedding box, which can avoid scratches during the sectioning process.

[0006] In order to achieve the above-mentioned purpose, the present invention provides the following technical solution: a new type of exfoliated cell embedding box, comprising a base, a cover body movably provided on the base, a protrusion provided on the cover body, a ring opening formed on the base, the size of the protrusion coincides with the ring opening, and when the cover body is buckled on the base, the protrusion is located in the ring opening.

[0007] Preferably, a bottom filter membrane is provided at the bottom of the ring opening, and a side filter membrane is provided on the side of the ring opening.

[0008] Preferably, the diameter of the ring opening is 1 cm.

[0009] Preferably, the thickness of the ring is the same as that of the base, and the thickness of the base is 0.5 cm.

[0010] Preferably, the bottom filter membrane and the side filter membrane are both made of polypropylene, are two-layered, and have a diameter of 2 μm.

[0011] Preferably, the height of the protrusion is 0.3 cm.

[0012] Preferably, a top filter membrane is provided on the protrusion.

[0013] Preferably, it comprises a top filter membrane and a bottom filter membrane, and when the protrusion is located in the ring opening, the distance between the top filter membrane and the bottom filter membrane is 0.2 cm.

[0014] In the above technical solution, the utility model provides a new type of exfoliated cell embedding box, which has the following beneficial effects: cell clusters are placed through the base, the base is buckled through the cover, the cell clusters are placed through the ring on the base, and the cell aggregation is ensured through the protrusion on the cover, which facilitates the subsequent cell cluster embedding work. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0016] Figure 1 A schematic diagram of the overall structure provided for an embodiment of the present utility model;

[0017] Figure 2A schematic diagram of the structure inside the mixing box and the feed port provided in an embodiment of the utility model;

[0018] Figure 3 A schematic structural diagram of a screening assembly provided in an embodiment of the present utility model.

[0019] Description of reference numerals:

[0020] 1. Cover; 11. Grid; 12. Protrusion; 2. Base; 21. Ring; 22. Bottom filter membrane; 23. Side filter membrane; 24. Top filter membrane; 241. Notch. DETAILED DESCRIPTION

[0021] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0022] like Figure 1-3 As shown, a new type of exfoliated cell embedding box includes a base 2, a cover body 1 is movably provided on the base 2, a protrusion 12 is provided on the cover body 1, and a ring opening 21 is opened on the base 2. The size of the protrusion 12 is consistent with the ring opening 21, and when the cover body 1 is buckled on the base 2, the protrusion 12 is located in the ring opening 21, and a notch 241 begins to appear on the ring opening 21.

[0023] Specifically, when in use, the cell cluster is placed in the ring opening 21 on the base 2, and then the cover body 1 is closed. At this time, the protrusion 12 on the cover body 1 is embedded in the groove 241 opened on the ring opening 21. When the cell cluster is located in the ring opening 21, it is prevented from being scraped into the embedding mold by the subsequent tweezers. This will easily bring cotton fibers into the cell cluster, and scratches will easily appear in the subsequent film preparation process, affecting the doctor's reading of the film.

[0024] In the above scheme, the cell cluster is placed through the base 2, the base 2 is buckled through the cover 1, the cell cluster is placed through the ring 21 on the base 2, and the cell aggregation is ensured through the protrusion 12 on the cover 1, which facilitates the subsequent cell cluster embedding work.

[0025] As an embodiment further provided by the present invention, according to Figure 1 、 Figure 2 and Figure 3 As shown, a bottom filter membrane 22 is provided at the bottom of the ring opening 21 , and a side filter membrane 23 is provided on the side of the ring opening 21 .

[0026] Furthermore, the diameter of the ring opening 21 is 1 cm.

[0027] Furthermore, the thickness of the ring 21 is the same as that of the base 2, and the thickness of the base 2 is 0.5 cm.

[0028] Furthermore, the bottom filter membrane 22 and the side filter membrane 23 are both made of polypropylene, and both the bottom filter membrane 22 and the side filter membrane 23 are two-layered, and have a diameter of 2 μm.

[0029] Furthermore, the height of the protrusion 12 is 0.3 cm.

[0030] Furthermore, a top filter membrane 24 is provided on the protrusion 12 .

[0031] Furthermore, it includes a top filter membrane 24 and a bottom filter membrane 22. When the protrusion 12 is located in the ring opening 21, the distance between the top filter membrane 24 and the bottom filter membrane 22 is 0.2 cm.

[0032] Specifically, the height of the ring 21 is the same as the thickness of the base 2, and the thickness of the base 2 is generally 0.5 cm. The bottom filter membrane 22 and the side filter membrane 23 are fixed to the bottom and four sides of the circular ring 21. The cover body 1 is provided with a protrusion 12 with a diameter of 0.05 cm at the position corresponding to the ring 21. A circular structure is made in the circular ring 21 of the cover body 1, which is 0.3 cm concave toward the bottom of the base 2. A top filter membrane 24 is attached to the circular structure. After the base 2 is covered, the height between the top filter membrane 24 and the bottom filter membrane 22 is 0.2 cm to ensure the effective aggregation of cell clusters and facilitate the subsequent cell cluster embedding work.

[0033] Working principle: First, place the cell cluster in the circle 21 on the base 2. The height of the circle 21 is the same as the thickness of the base 2 (the thickness of the base 2 is generally 0.5 cm). Fix the bottom filter membrane 22 and the side filter membrane 23 to the bottom and around the circular circle 21. Place the cover 1 with a protrusion 12 with a diameter of 0.05 cm at the position of the circle 21. Make a circular structure 0.3 cm concave toward the bottom of the base 2 in the circular circle 21 of the cover 1. Attach a top filter membrane 24 to the circular structure. After the base 2 cover is covered, the height between the top filter membrane 24 and the bottom filter membrane 22 is 0.2 cm to ensure the effective aggregation of the cell clusters and facilitate the subsequent cell cluster embedding work. Transfer the cell clusters collected by centrifugation into the circular space with a pipette, cover the cover 1 and start the subsequent operation. After dehydration, a cell wax block is formed.

[0034] The above description is merely illustrative of certain exemplary embodiments of the present invention. It goes without saying that those skilled in the art will be able to modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and description are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A novel exfoliated cell embedding cassette, characterized in that: The invention comprises a base (2), a cover body (1) movably arranged on the base (2), a protrusion (12) arranged on the cover body (1), a ring opening (21) opened on the base (2), the size of the protrusion (12) matches the ring opening (21), and when the cover body (1) is buckled on the base (2), the protrusion (12) is located in the ring opening (21), and a notch (241) begins to be formed on the ring opening (21).

2. A novel exfoliated cell embedding cassette according to claim 1, characterized in that: A bottom filter membrane (22) is arranged at the bottom of the ring opening (21), and a side filter membrane (23) is arranged on the side of the ring opening (21).

3. A novel exfoliated cell embedding cassette according to claim 2, characterized in that: The diameter of the ring opening (21) is 1 cm.

4. A novel exfoliated cell embedding cassette according to claim 1, characterized in that: The thickness of the ring opening (21) is the same as that of the base (2), and the thickness of the base (2) is 0.5 cm.

5. A novel exfoliated cell embedding cassette according to claim 2, characterized in that: The bottom filter membrane (22) and the side filter membrane (23) are both made of polypropylene, and both the bottom filter membrane (22) and the side filter membrane (23) are double-layered, and have a diameter of 2 um.

6. A novel exfoliated cell embedding cassette according to claim 1, characterized in that: The height of the protrusion (12) is 0.3 cm.

7. A novel exfoliated cell embedding cassette according to claim 6, characterized in that: A top filter membrane (24) is arranged on the protrusion (12).

8. A novel exfoliated cell embedding cassette according to claim 1, characterized in that: It comprises a top filter membrane (24) and a bottom filter membrane (22). When the protrusion (12) is located in the ring opening (21), the distance between the top filter membrane (24) and the bottom filter membrane (22) is 0.2 cm.

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