Constant-temperature water bath kettle for cell resuscitation

Abstract

The utility model discloses a constant-temperature water bath kettle for cell resuscitation, which comprises a water bath kettle main body, an annular water bath is arranged on the water bath kettle main body, a circular groove positioned in the middle of the water bath kettle main body is arranged on the inner side of the annular water bath, a double-layer bracket is arranged in the circular groove, and a positive and negative rotation driving motor is arranged on the bottom surface of the double-layer bracket. A driving gear located on the top face of the double-layer support is arranged at the output end above the forward and reverse rotation driving motor, a rotating disc is arranged outside the driving gear, a tooth-shaped face matched with the driving gear is arranged on the inner wall face of the rotating disc, a set of annularly-distributed supports are arranged on the outer side of the rotating disc, and an annular containing frame located above the annular water bath is arranged on the supports. And a group of cryopreservation tubes in clearance fit are arranged in the annular placement frame. The utility model has the characteristic that the cell thawing and resuscitation speed can be effectively improved.

Description

Technical Field

[0001] The utility model relates to a water bath pot, in particular to a cell resuscitation constant temperature water bath pot. Background Art

[0002] In order to avoid contamination caused by long-term in vitro culture of cultured cells or gene mutation caused by multiple passages, the experimenter will freeze the cultured cells during the vigorous growth period. The frozen cells are stored in cell cryopreservation tubes with cell freezing solution, and then placed in liquid nitrogen at -196℃. In this ultra-low temperature environment, the cells can be stored for a long time. When the experiment requires, the experimenter can revive the frozen cells in a 37℃ water bath to conduct the required experiment, which can maximize the genetic stability of the cells.

[0003] At present, the commonly used method of thawing frozen cells is to place the cell cryopreservation tubes in a 37°C constant temperature water bath for thawing. However, the current constant temperature water bath is generally a round pot, with a tray set on the edge of the water bath, and the cryopreservation tubes on the tray are placed in the water bath for cell thawing. During this process, the cryopreservation tubes are stationary, resulting in a slow rate of thawing and thawing of the cells in the cryopreservation tubes. Therefore, it is urgent to develop a constant temperature water bath that can increase the rate of cell thawing and thawing. Utility Model Content

[0004] The purpose of the utility model is to provide a cell resuscitation constant temperature water bath, which has the characteristic of effectively improving the cell melting and resuscitation speed.

[0005] The technical solution of the utility model is as follows: a cell recovery constant temperature water bath comprises a water bath body, an annular water bath tank is arranged on the water bath body, a circular groove located in the middle of the water bath body is arranged on the inner side of the annular water bath tank, a double-layer bracket is arranged in the circular groove, a forward and reverse driving motor is arranged on the bottom surface of the double-layer bracket, a driving gear located on the top surface of the double-layer bracket is arranged on the output end above the forward and reverse driving motor, a rotating disk is arranged outside the driving gear, a toothed surface matching the driving gear is arranged on the inner wall surface of the rotating disk, a group of annularly distributed brackets are arranged on the outer side of the rotating disk, an annular placement rack located above the annular water bath tank is arranged on the bracket, and a group of cryopreservation tubes with clearance fit are arranged in the annular placement rack.

[0006] In the aforementioned cell recovery constant temperature water bath, two symmetrically distributed electric lifting rods are provided on the left and right sides between the inner bottom surface of the circular groove and the double-layer bracket, and the electric lifting rods are rotatably connected to the double-layer bracket; follow-up telescopic rods are also provided on the front and rear sides between the double-layer bracket and the inner bottom surface of the circular groove, and the follow-up telescopic rods are also rotatably connected to the double-layer bracket.

[0007] In the aforementioned cell recovery constant temperature water bath, the bracket is provided with a plurality of limiting grooves distributed side by side, and the limiting grooves are embedded with suction magnets; the annular placement frame is provided with a clamping groove corresponding to the position of the limiting groove, and the clamping groove is embedded with a magnetic body that cooperates with the suction magnet.

[0008] In the aforementioned cell recovery constant temperature water bath, the annular placement rack includes a top annular rack, a middle rack and a bottom rack located between two adjacent racks are arranged below the top annular rack, a top hole is arranged on the top annular rack, a middle hole is arranged on the middle rack, and a bottom conical hole is arranged on the bottom rack, and the apertures of the top hole and the middle rack are larger than the diameter of the cryopreservation tube, and the aperture of the bottom of the bottom conical hole is smaller than the diameter of the cryopreservation tube; the card slot is located on the lower surface of the top annular rack, and the card slot is located between adjacent bottom racks.

[0009] Compared with the prior art, the utility model provides an annular water bath, which cooperates with an annular placement rack that can rotate forward and reverse, so that the cryotubes can move in a circle along the annular water bath, causing disturbance to the water in the annular water bath groove, thereby ensuring the uniformity of the water bath temperature; and there is a clearance fit between the cryotubes and the annular placement rack, which will collide with the water during the regular forward and reverse rotation process, causing a slight shake, thereby promoting the melting of cells in the cryotubes and increasing the speed of cell recovery. In addition, the present application provides two symmetrically distributed electric lifting rods in the circular groove, which can drive the double-layer bracket to move up and down, drive the cryotubes to shake slightly, and further promote the melting of cells in the cryotubes. In summary, the utility model has the characteristics of being able to effectively improve the speed of cell melting and recovery. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 It is a structural schematic diagram of the utility model;

[0011] Figure 2 This is a structural view after omitting the ring placement rack;

[0012] Figure 3 It is a partial view of the ring-shaped storage rack.

[0013] The marks in the accompanying drawings are: 1-water bath body, 2-annular water bath, 3-circular groove, 4-double-layer bracket, 5-forward and reverse drive motor, 6-drive gear, 7-rotating disk, 8-bracket, 9-annular placement rack, 10-freezing tube, 11-electric lifting rod, 16-follow-up telescopic rod, 12-limiting groove, 13-attractive magnet, 14-card slot, 15-magnetic body, 901-top annular rack, 902-middle rack, 903-bottom rack, 904-top hole, 905-middle hole, 906-bottom conical hole. DETAILED DESCRIPTION

[0014] The present invention is further described below in conjunction with the accompanying drawings and embodiments, but they are not intended to limit the present invention.

[0015] Example. A cell recovery constant temperature water bath, comprising: Figure 1-3 As shown, it includes a water bath body 1, on which is an annular water bath 2, on which is an inner side of the annular water bath 2 a circular groove 3 located in the middle of the water bath body 1 is provided, in which is a double-layer bracket 4, on which is a forward and reverse drive motor 5 a forward and reverse drive motor 5 a forward and reverse drive motor 6 located on the top of the double-layer bracket 4 is provided at the output end above the forward and reverse drive motor 5, on which is a rotating disk 7 a rotating disk 7 a toothed surface matching the driving gear 6 is provided on the inner wall surface of the rotating disk 7, on which is a group of annularly distributed brackets 8 a ring-shaped placement rack 9 located above the annular water bath 2 is provided on the bracket 8, in which is a group of cryopreservation tubes 10 with clearance fit are provided.

[0016] Two symmetrically distributed electric lifting rods 11 are provided between the inner bottom surface of the circular groove 3 and the double-layer bracket 4, and the electric lifting rods 11 are rotatably connected to the double-layer bracket 4; follow-up telescopic rods 16 are also provided on the front and rear sides between the double-layer bracket 4 and the inner bottom surface of the circular groove 3, and the follow-up telescopic rods 16 are also rotatably connected to the double-layer bracket 4.

[0017] like Figure 2 As shown, the bracket 8 is provided with a plurality of limit grooves 12 distributed side by side, and the limit grooves 12 are embedded with suction magnets 13; the annular placement rack 9 is provided with a clamping groove 14 corresponding to the position of the limit groove 12, and the clamping groove 14 is embedded with a magnetic body 15 that cooperates with the suction magnet 13.

[0018] like Figure 3 As shown, the annular placement rack 9 includes a top annular rack 901, a middle rack 902 and a bottom rack 903 located between two adjacent racks 8 are provided below the top annular rack 901, a top hole 904 is provided on the top annular rack 901, a middle hole 905 is provided on the middle rack 902, and a bottom conical hole 906 is provided on the bottom rack 903, and the apertures of the top hole 904 and the middle rack 902 are larger than the diameter of the cryopreservation tube, and the aperture of the bottom of the bottom conical hole 906 is smaller than the diameter of the cryopreservation tube; the card slot 14 is located on the lower surface of the top annular rack 901, and the card slot 14 is located between adjacent bottom racks 903.

[0019] An annular electric heating wire is arranged below the annular water bath.

[0020] The annular water bath is filled with water. Water can be added externally by the operator.

[0021] Preferably, a drain valve may be provided at the bottom of one side of the annular water bath to discharge the water in the annular water bath.

[0022] The diameter of the upper part of the bottom conical hole is slightly larger than the diameter of the test tube, and the diameter of the lower part of the bottom conical hole is smaller than the diameter of the test tube. In this way, the test tube can be received.

[0023] The top hole is lined with a soft silicone liner.

[0024] Vertical brackets are arranged between the two ends of the middle frame and the bottom frame and the top annular frame.

[0025] By arranging a plurality of limiting grooves on the bracket, the annular placement rack is fixed by magnetism, and the installation is flexible and convenient. A corresponding number of annular placement racks can be flexibly installed according to the actual need for resuscitated cells.

[0026] The working process of the utility model is as follows: the electric heating wire is turned on to heat the water in the annular water bath until it is heated to a set temperature and maintained; then the cryotubes that need to revive cells are placed on the corresponding annular placement rack, and the annular placement rack is magnetically fixed to the bracket limit groove at the corresponding position, and a part of the cryotube is immersed in water for revival; then, the forward and reverse drive motor works to drive the drive gear to rotate regularly in the forward and reverse directions (controlling a slower rotation speed), thereby driving the rotating disk to rotate, and the rotation of the rotating disk drives the annular placement rack and its cryotubes to rotate regularly in the forward and reverse directions, so that the cryotubes rotate in the annular water bath, promote water flow, and ensure that the water temperature in the annular water bath remains constant; in this process, since there is a certain gap between the cryotube and the top hole and the middle hole, it will swing around the bottom of the cryotube during rotation, forming a smaller swing, which is beneficial to promote the melting of cells in the cryotube and increase the speed of cell recovery. At the same time, one of the electric lifting rods at the bottom of the double-layer bracket rises, and the other electric lifting rod falls synchronously, and so on (note: the rising and falling distances are short and the frequency is low), driving the annular placement rack to rotate around the center line formed by the front and rear follow-up telescopic rods, so that the cryopreservation tubes on the annular placement rack shake slightly, thereby promoting the melting speed of cells inside the cryopreservation tubes and increasing the cell melting recovery speed.

Claims

1. Cell recovery constant temperature water bath, characterized by: The invention comprises a water bath body (1), wherein an annular water bath tank (2) is provided on the water bath body (1), a circular groove (3) located in the middle of the water bath body (1) is provided on the inner side of the annular water bath tank (2), a double-layer bracket (4) is provided in the circular groove (3), a forward and reverse driving motor (5) is provided on the bottom surface of the double-layer bracket (4), a driving gear (6) located on the top surface of the double-layer bracket (4) is provided on the output end above the forward and reverse driving motor (5), a rotating disk (7) is provided outside the driving gear (6), a toothed surface matching the driving gear (6) is provided on the inner wall surface of the rotating disk (7), a group of annularly distributed brackets (8) are provided on the outer side of the rotating disk (7), an annular placement rack (9) located above the annular water bath tank (2) is provided on the bracket (8), and a group of cryopreservation tubes (10) with clearance fit are provided in the annular placement rack (9).

2. The cell resuscitation constant temperature water bath according to claim 1, characterized in that: Two symmetrically distributed electric lifting rods (11) are arranged between the inner bottom surface of the circular groove (3) and the double-layer bracket (4), and the electric lifting rods (11) and the double-layer bracket (4) are rotatably connected; follow-up telescopic rods (16) are also arranged on the front and rear sides between the double-layer bracket (4) and the inner bottom surface of the circular groove (3), and the follow-up telescopic rods (16) and the double-layer bracket (4) are also rotatably connected.

3. The cell resuscitation constant temperature water bath according to claim 2, characterized in that: The bracket (8) is provided with a plurality of limit grooves (12) distributed side by side, and an attracting magnet (13) is embedded in the limit groove (12); the annular placement frame (9) is provided with a clamping groove (14) corresponding to the position of the limit groove (12), and a magnetic body (15) matching the attracting magnet (13) is embedded in the clamping groove (14).

4. The cell resuscitation constant temperature water bath according to claim 3, characterized in that: The annular placement rack (9) comprises a top annular rack (901), a middle rack (902) and a bottom rack (903) located between two adjacent racks (8) are arranged below the top annular rack (901), a top hole (904) is arranged on the top annular rack (901), a middle hole (905) is arranged on the middle rack (902), and a bottom conical hole (906) is arranged on the bottom rack (903), and the apertures of the top hole (904) and the middle rack (902) are larger than the diameter of the cryopreservation tube, and the aperture of the bottom of the bottom conical hole (906) is smaller than the diameter of the cryopreservation tube; the card slot (14) is located on the lower surface of the top annular rack (901), and the card slot (14) is located between adjacent bottom racks (903).

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