Extraction device for batch recovery of nucleic acid

Abstract

The utility model relates to the technical field of nucleic acid detection, in particular to an extraction device for batch recovery of nucleic acid, which comprises an extraction tube and a separation box, the extraction tube comprises a tube wall, an upper filter element and a lower filter element are respectively arranged at the upper end and the lower end of the tube wall; the lower filter assembly comprises a pressing ring, a glass fiber filter element and a lower filter element which are arranged from top to bottom, the separation box comprises a box body and a connecting pipe arranged in the box body, a plurality of placement grooves are formed in the box body in a linear array mode, clamping grooves are formed between the longitudinally adjacent placement grooves, the connecting pipe comprises a plurality of storage pipes, and the storage pipes are connected with the glass fiber filter element. A limiting block is arranged between every two adjacent storage pipes. According to the utility model, during large-batch extraction, the extraction can be completed through a pipette or multi-channel liquid sucking and beating equipment, and the equipment does not need to be equipped with the functions of oscillation, magnetic suction and the like used in a magnetic bead method, so that the operation is simpler and more convenient, and the operability is stronger.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid detection, and particularly to an extraction device for batch recovery of nucleic acids. Background Art

[0002] In current molecular diagnostic experiments, magnetic bead extraction and column extraction are the two most commonly used methods. The magnetic bead extraction method can realize automated and large-scale experimental operations, but the magnetic beads and the supporting automatic extraction workstation are expensive; the column extraction method often uses silicon materials as solid phase carriers, such as silica gel, glass fiber, etc., which are low-cost, easy to operate, and have high extraction quality, but there are problems with automation and large-scale extraction. Utility Model Content

[0003] The utility model provides an extraction device for batch recovery of nucleic acids to solve the problems of the prior art.

[0004] The purpose of the utility model can be achieved through the following technical scheme: an extraction device for batch recovery of nucleic acid, comprising:

[0005] An extraction tube, the extraction tube comprising a tube wall, an upper filter element and a lower filter assembly are respectively arranged at the upper and lower ends of the tube wall, and the lower filter assembly comprises a pressing ring, a glass fiber filter element and a lower filter element arranged from top to bottom;

[0006] A separation box, comprising a box body and a connecting tube arranged in the box body, wherein a plurality of placement grooves are arranged in a linear array in the box body, a clamping groove is arranged between the longitudinally adjacent placement grooves, and the connecting tube comprises a plurality of storage tubes, and a limiting block is arranged between adjacent storage tubes.

[0007] As a further improvement, an inverted cone-shaped extraction portion is provided at the bottom end of the extraction tube, and a liquid intake port is obliquely provided at the bottom end of the inverted cone-shaped extraction portion.

[0008] As a further improvement, a fixing groove is provided at the lower end of the separation box.

[0009] As a further improvement, the outer side of the upper end of the extraction tube is provided with anti-slip grooves.

[0010] As a further improvement, the number of storage tubes in each group of the connecting tubes is .

[0011] As a further improvement, the number of the placement slots in the transverse direction is 12, and the number of the placement slots in the longitudinal direction is 8.

[0012] Compared with the prior art, the utility model has the following beneficial effects:

[0013] 1. The utility model is easier to operate and more operable. When extracting in large quantities, it can be completed by a row gun or a multi-channel liquid suction and liquid pumping device. The equipment does not need to be equipped with the functions of shaking, magnetic suction, etc. used in the magnetic bead method;

[0014] 2. The utility model adopts a double filter element structure, and after double-layer filtration, the extracted nucleic acid has a higher purity;

[0015] 3. The utility model connects the tube and is inserted into the placement slot of the separation box through a limit block. During use, the nucleic acid reagent is directly eluted in the connect, and after elution, it is directly taken out and covered for storage. The nucleic acid recovery rate is high, which avoids the disadvantages of large loss, large residue and difficult recovery during the collection process of the eluent when eluting directly in the separation box. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the structure of the utility model;

[0017] Figure 2 It is a top view of the separation box of the utility model;

[0018] Figure 3 It is a cross-sectional view of the extraction tube of the utility model;

[0019] Figure 4 For this utility model Figure 1 A magnified view of the part A in the middle;

[0020] Figure 5 For this utility model Figure 3 Enlarged view of part B in the middle.

[0021] In the figure, 1. extraction tube; 11. tube wall; 111. anti-slip pattern; 12. upper filter element; 13. lower filter assembly; 131. pressing ring; 132. glass fiber filter element; 133. lower filter element; 2. separation box; 21. box body; 211. placement groove; 212. snap-on groove; 22. connecting tube; 221. storage tube; 222. limit block; 23. fixing groove; 31. inverted cone-shaped extraction part; 32. liquid taking port. DETAILED DESCRIPTION

[0022] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as limitations on the present invention.

[0023] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0024] The following is combined with the embodiments and attached Figures 1 to 5 , the technical solution of the utility model is further elaborated.

[0025] Example 1

[0026] An extraction device for recovering nucleic acid in batches, comprising:

[0027] The extraction tube 1 comprises a tube wall 11, and an upper filter element 12 and a lower filter assembly 13 are respectively arranged at the upper and lower ends of the tube wall 11, and the lower filter assembly 13 comprises a pressing ring 131, a glass fiber filter element 132 and a lower filter element 133 arranged from top to bottom;

[0028] The separation box 2 comprises a box body 21 and a connecting tube 22 arranged in the box body 21. A plurality of placement grooves 211 are arranged in a linear array in the box body 21. A clamping groove 212 is arranged between the longitudinally adjacent placement grooves 211. The connecting tube 22 comprises a plurality of storage tubes 221. Limiting blocks 222 are arranged between adjacent storage tubes 221.

[0029] like Figures 1 to 5 As shown, the glass fiber filter element 132 in the extraction tube 1 of the utility model is a porous material with a large number of hydrophilic groups on its surface, which can form hydrogen bonds with water molecules. Negatively charged nucleic acids have a high affinity for positively charged glass fibers. In high salt and ethanol solutions, nucleic acids bind to glass fibers and interact with the hydrophilic matrix on silica through hydrogen bonds. The nucleic acids are tightly bound, and large volume solution washing can remove all contaminants.

[0030] The specific steps are as follows:

[0031] S1: according to the use requirements, the lysate, the first washing solution, the second washing solution and the third washing solution are sequentially injected into the placement slot 211 of the separation box 2, the connecting tube 22 is placed in the empty placement slot 211 and the corresponding volume of the eluent is added;

[0032] S2: injecting the nucleic acid sample to be extracted into the lysis solution of the first column;

[0033] S3: Use extraction tube 1 to repeatedly pipette the mixed sample of nucleic acid sample and lysis solution;

[0034] S4: repeatedly aspirate and pump the extraction tube 1 in the first washing liquid, the second washing liquid and the third washing liquid for several times;

[0035] S5: repeatedly pipetting the extraction tube 1 in the connecting tube 22 for several times to obtain a high-purity nucleic acid eluate;

[0036] S6: After the linked tube 22 is capped, it is taken out from the separation box 2 and can be frozen for storage or for PCR experiment.

[0037] The extraction tube 1 of the utility model is filled with a glass fiber filter 132, and the nucleic acid in the lysed sample is firmly adsorbed on the surface of the glass fiber filter by repeated aspiration and discharge, and then washed by repeated aspiration and discharge, and finally a small amount of low-salt solution is absorbed to elute the nucleic acid on the surface of the glass fiber filter to obtain a high-purity nucleic acid solution.

[0038] The snap-fit ​​groove 212 in the box body 21 can be buckled with the connecting tube 22 through the limit block 222, and can be easily disassembled. After the extraction is completed, the nucleic acid eluent is directly injected into the connecting tube 22, covered, and then removed for storage, avoiding the process of collecting the eluent hole by hole with a pipette gun after the traditional magnetic bead extraction is completed.

[0039] As a further preferred embodiment, an inverted cone-shaped extraction portion 31 is provided at the bottom of the extraction tube 1, and a liquid collection port 32 is obliquely provided at the bottom of the inverted cone-shaped extraction portion 31. The obliquely provided liquid collection port 32 facilitates the detection personnel to collect the reagent.

[0040] As a further preferred embodiment, a fixing groove 23 is provided at the lower end of the separation box 2 to facilitate the later transportation and fixing of the separation box 2.

[0041] As a further preferred embodiment, an anti-slip pattern 111 is provided on the outer side of the upper end of the extraction tube 1 to facilitate the inspection personnel to grasp the extraction tube 1 .

[0042] As a further preferred embodiment, the number of storage tubes 221 in each group of the connecting tubes 22 is 8.

[0043] As a further preferred embodiment, the number of the placement slots 211 in the transverse direction is 12, and the number of the placement slots 211 in the longitudinal direction is 8.

[0044] The preferred specific embodiments of the utility model are described in detail above. It should be understood that ordinary technicians in this field can make many modifications and changes based on the concept of the utility model without creative work. Therefore, all technical solutions that can be obtained by technicians in this technical field based on the concept of the utility model through logical analysis, reasoning or limited experiments on the basis of the existing technology should be within the scope of protection determined by the claims.

Claims

1. An extraction device for recovering nucleic acid in batches, characterized in that: include: An extraction tube (1), the extraction tube (1) comprising a tube wall (11), an upper filter element (12) and a lower filter assembly (13) being respectively arranged at the upper and lower ends of the tube wall (11), the lower filter assembly (13) comprising a pressing ring (131), a glass fiber filter element (132) and a lower filter element (133) arranged from top to bottom; A separation box (2), the separation box (2) comprising a box body (21) and a connecting tube (22) arranged in the box body (21), a plurality of placement grooves (211) being arranged in a linear array in the box body (21), a clamping groove (212) being arranged between the longitudinally adjacent placement grooves (211), the connecting tube (22) comprising a plurality of storage tubes (221), and a limiting block (222) being arranged between the adjacent storage tubes (221).

2. The extraction device for recovering nucleic acid in batches according to claim 1, characterized in that: An inverted cone-shaped extraction portion (31) is provided at the bottom end of the extraction tube (1), and a liquid extraction port (32) is obliquely provided at the bottom end of the inverted cone-shaped extraction portion (31).

3. The extraction device for recovering nucleic acid in batches according to claim 1, characterized in that: The lower end of the separation box (2) is provided with a fixing groove (23).

4. The extraction device for recovering nucleic acid in batches according to claim 1, characterized in that: The outer side of the upper end of the extraction tube (1) is provided with anti-slip grooves (111).

5. The extraction device for recovering nucleic acid in batches according to claim 1, characterized in that: The number of storage tubes (221) in each group of the connecting tubes (22) is 8.

6. The extraction device for recovering nucleic acid in batches according to claim 5, characterized in that: The number of the placement slots (211) in the horizontal direction is 12, and the number of the placement slots (211) in the vertical direction is 8.

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