Cell filtering device and cell analysis equipment

Abstract

The utility model belongs to the technical field of biological science and discloses a cell filtering device and cell analysis equipment. The cell filtering device provided by the utility model comprises the microwell plate and the cell filtering sieves, the microwell plate is provided with the plurality of placing areas, each placing area corresponds to one cell filtering sieve, an operator can select the specification and the number of the cell filtering sieves by himself / herself according to use requirements, waste is avoided, and the cell filtering device is simple in structure and convenient to use. And a plurality of cell filter sieves can be randomly combined with the microporous plate, so that the flexibility is high. The cell filter sieve comprises a filter sieve body, a filter screen and a plurality of flow guide parts, and cells or cell clusters larger than a preset diameter in the suspension are filtered out by the filter screen, so that the suspension only containing target cells is obtained. According to the cell analysis equipment, by applying the cell filtering device, the cell analysis instrument is prevented from being blocked, and the flexibility and universality of the cell filtering sieve are improved, so that an operator can flexibly select cell filtering sieves with different specifications and numbers, and the test cost is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of biological science, in particular to a cell filtering device and a cell analysis device. Background Art

[0002] In the field of biological science and technology, when conducting experiments on cells, the experimental results are often unsatisfactory or even fail due to cell clumping, and it is easy to cause the instrument to be blocked. Therefore, it is necessary to filter the suspension first to remove the cell clumps and leave the single cells or other biological particles for testing.

[0003] However, the cell filter screen of the existing cell filtration device is usually an integral structure that cannot be disassembled and can only be applied to a specific type of microplate. It has low flexibility and needs to be replaced as a whole when the cell filter screen is damaged, which greatly increases the experimental cost.

[0004] Therefore, there is an urgent need to provide a cell filtration device and a cell analysis device to solve the above problems. Utility Model Content

[0005] The first purpose of the utility model is to provide a cell filtering device, which can be flexibly assembled or disassembled according to use requirements and can reduce the test cost.

[0006] The second object of the utility model is to provide a cell analysis device, which can filter out cells or cell agglomerates larger than a preset diameter by applying the above-mentioned cell filtering device, thereby improving the accuracy of cell analysis and avoiding clogging of the cell analysis instrument.

[0007] To achieve this purpose, the utility model adopts the following technical solutions:

[0008] A cell filtration device, comprising:

[0009] A microplate, wherein a plurality of placement areas are provided on the microplate, and each of the placement areas is provided with a plurality of collection holes arranged in an array;

[0010] A cell filter screen, each of the placement areas corresponds to a cell filter screen, the cell filter screen comprises a filter screen body, a filter screen and a plurality of guide parts, each of the guide parts is provided with a through guide hole, the filter screen body is provided with liquid collecting holes corresponding to the collection holes in the corresponding placement area, each of the liquid collecting holes is connected to a guide part, and the liquid collecting hole is arranged opposite to the guide hole of the corresponding guide part, each of the guide parts can be inserted into the corresponding collection hole, and each of the guide holes is provided with the filter screen.

[0011] Furthermore, each of the filter screen bodies and the multiple flow guides thereon are an integrally formed structure.

[0012] Furthermore, the liquid collecting holes on each of the filter screen bodies are arranged in an M×N array, wherein 1≤M≤12, 1≤N≤8.

[0013] Furthermore, a guide slope is provided at one end of the guide portion away from the filter screen body, and the guide slope gradually inclines toward the guide hole along a direction away from the filter screen body.

[0014] Furthermore, the filter screen is bonded or hot-pressed to the flow guide portion.

[0015] Furthermore, the filter screen is made of nylon material.

[0016] Furthermore, the filter screen body is made of polyester material; and / or the flow guide portion is made of polyester material.

[0017] Furthermore, the pore size of the filter is 0.1 to 200 microns.

[0018] Furthermore, the height of the guide portion is 1 / 5 to 3 / 5 of the depth of the collecting hole.

[0019] A cell analysis device comprises a cell analysis instrument and a cell filtering device in any of the above schemes, wherein the cell filtering device is configured to filter out cells or cell agglomerates larger than a preset diameter in a suspension to obtain target cells, and the cell analysis instrument is used to obtain cell parameters of the target cells.

[0020] Beneficial effects of the utility model:

[0021] The utility model provides a cell filtering device, which comprises a microporous plate and a cell filtering screen. A plurality of placement areas are arranged on the microporous plate, each placement area is provided with a plurality of collecting holes arranged in an array, and the collecting holes are used to collect the suspension filtered by the cell filtering screen. Each placement area corresponds to a cell filtering screen. By arranging the cell filtering screen corresponding to each placement area, an operator can select the specification and quantity of the cell filtering screen according to the use requirements, so as to avoid waste. Moreover, the operator can arbitrarily combine a plurality of cell filtering screens with the microporous plate, which has high flexibility. The cell filter screen includes a filter screen body, a filter screen and multiple guide parts, each of which is provided with a through guide hole, and the filter screen body is provided with liquid collecting holes corresponding to the collecting holes in the corresponding placement area. The operator injects the suspension into the guide holes through the liquid collecting holes. Each collecting hole is connected to a guide part, and the collecting hole is arranged opposite to the guide hole of the corresponding guide part, so that the guide part is arranged circumferentially around the collecting hole to guide the flow direction of the suspension. Each guide part can be inserted into the corresponding collecting hole, so that the position of the cell filter screen and the microporous plate is relatively fixed to avoid misalignment. A filter screen is provided in each guide hole, and the suspension injected into the guide hole enters the collecting hole through the filter screen under the action of gravity or the blowing force of the pipette gun. Cells in the suspension larger than the preset diameter are filtered out by the filter screen in the above process, thereby obtaining a suspension containing only target cells.

[0022] The utility model also provides a cell analysis device, comprising a cell analysis instrument and the above-mentioned cell filtering device, wherein the cell filtering device is configured to filter out cells or cell agglomerates larger than a preset diameter in a suspension to obtain target cells, and the cell analysis instrument is used to obtain cell parameters of the target cells. By applying the above-mentioned cell filtering device, the cell analysis instrument is prevented from being blocked by cells larger than a preset diameter, thereby improving the flexibility and versatility of the cell filtering screen, so that operators can flexibly select cell filtering screens of different specifications and quantities according to usage requirements, thereby reducing waste and lowering test costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a structural schematic diagram of a microporous plate and a cell filter screen of a certain specification provided by the utility model;

[0024] Figure 2 It is a half-section schematic diagram of a microplate and a cell filter screen of a specification provided by the utility model;

[0025] Figure 3 This is a schematic diagram of the structure of a cell filter screen of a certain specification provided by the utility model;

[0026] Figure 4 It is a structural schematic diagram of a microporous plate and a cell filter screen of another specification provided by the utility model.

[0027] In the figure:

[0028] 1. Microplate; 11. Collection hole;

[0029] 2. Cell filter screen; 21. Filter screen body; 211. Liquid collecting hole; 22. Diversion part; 221. Diversion hole; 23. Filter screen. DETAILED DESCRIPTION

[0030] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It is to be understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention. It should also be noted that, for ease of description, only the parts related to the present invention, rather than all structures, are shown in the accompanying drawings.

[0031] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0032] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0033] In the description of this embodiment, the terms "upper", "lower", "right", etc., are based on the directions or positions shown in the drawings, and are only for the convenience of description and simplified operation, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation of the present invention. In addition, the terms "first" and "second" are only used to distinguish in the description and have no special meaning.

[0034] This embodiment provides a cell filter device for filtering out cells or cell agglomerates larger than a preset diameter in a suspension, thereby obtaining dispersed target cells for analysis of the target cells. The cell filter screen of an existing cell filter device is usually an integral structure that cannot be disassembled, has low flexibility, and is easily wasted during use.

[0035] To solve the above problems, Figure 1 to Figure 3 As shown, the cell filtration device provided in this embodiment includes a microporous plate 1 and a cell filtration screen 2. A plurality of placement areas are provided on the microporous plate 1. Each placement area is provided with a plurality of collecting holes 11 arranged in an array. The collecting holes 11 are used to collect the suspension filtered by the cell filtration screen 2. Each placement area corresponds to a cell filtration screen 2. By providing a cell filtration screen 2 corresponding to each placement area, the operator can select the specification and quantity of the cell filtration screen 2 according to the use requirements to avoid waste. In addition, the operator can arbitrarily combine a plurality of cell filtration screens 2 with the microporous plate 1, which is highly flexible. The cell filter sieve 2 includes a filter sieve body 21, a filter screen 23 and a plurality of guide parts 22, each guide part 22 is provided with a through guide hole 221, the filter sieve body 21 is provided with a liquid collecting hole 211 corresponding to the collection hole 11 in the corresponding placement area, the operator injects the suspension into the guide hole 221 through the liquid collecting hole 211, each liquid collecting hole 211 is connected to a guide part 22, and the liquid collecting hole 211 is arranged opposite to the guide hole 221 of the corresponding guide part 22, so that the guide part 22 is surrounded by The circumferential arrangement of the liquid collecting holes 211 can guide the flow direction of the suspension. Each guide portion 22 can be inserted into the corresponding collecting hole 11, so that the position of the cell filter screen 2 and the microplate 1 is relatively fixed to avoid misalignment. A filter screen 23 is arranged in each guide hole 221. The suspension injected into the guide hole 221 enters the collecting hole 11 through the filter screen 23 under the action of gravity or the blowing force of the pipette gun. Cells in the suspension larger than the preset diameter are filtered out by the filter screen 23 in the above process, thereby obtaining a suspension containing only target cells.

[0036] For ease of processing, the filter screen body 21 and the multiple guide parts 22 thereon are an integrally formed structure. At the same time, the integrally formed structure makes the connection between the filter screen body 21 and the guide parts 22 stable and not easy to separate, and avoids the existence of a gap between the filter screen body 21 and the guide parts 22, which causes the suspension to leak from the gap and contaminate the adjacent collection holes 11, thereby ensuring the purity of the cells in each collection hole 11.

[0037] Corresponding to the collection holes 11 arranged in an array on the placement area, the liquid collection holes 211 on each filter body 21 are arranged in an M×N array, where 1≤M≤12, 1≤N≤8. In this embodiment, the microplate 1 is a 96-well plate, M=12, N=1, and the operator can choose to install 1 to 8 cell filter screens 2 on the microplate 1 for filtering according to the use requirements. In other embodiments, such as Figure 4 As shown, for microplates 1 with different numbers of holes or placement areas with different numbers of holes to be used, the numbers of M and N can be adaptively adjusted. Optionally, for microplates 1 with more holes, the values ​​of M and N can be adaptively adjusted to fit microplates 1 of various specifications.

[0038] Furthermore, a guide slope (not shown in the figure) is provided at one end of the guide part 22 away from the filter screen body 21, and the guide slope gradually tilts toward the guide hole 221 in a direction away from the filter screen body 21, thereby facilitating the insertion of the guide part 22 into the collecting hole 11.

[0039] Specifically, the filter screen 23 is bonded or hot-pressed to the bottom of the guide portion 22. This connection method is simple to operate and low in cost, so that the filter screen 23 is stably connected to the guide portion 22, preventing the filter screen 23 from falling off and affecting the filtering effect. In other embodiments, laser welding or other methods can be selected to connect the filter screen 23 and the guide portion 22, which is not limited here. Optionally, a plurality of filter screens 23 can also be provided on the guide portion 22, and the plurality of filter screens 23 are respectively connected to the upper part, the middle part or the bottom part of the guide portion 22 to achieve multi-stage filtration, thereby providing a better filtering effect.

[0040] In this embodiment, the filter screen 23 is made of nylon material. In the prior art, the manufacturing precision of the size of nylon material is high, which is conducive to improving the filtering precision of the cell filtering device, and is suitable for filtering cells of various diameters, and is also conducive to reducing the production cost of the cell filter screen 2. In other embodiments, the material of the filter screen 23 includes but is not limited to polyester fiber, polypropylene or polyurethane, etc., which is not limited here.

[0041] Specifically, the filter screen body 21 is made of polyester material; and / or the flow guide portion 22 is made of polyester material, which has a lower cost.

[0042] In order to filter cells of various diameters, the pore size of the filter 23 can be selected to be 0.1 to 200 microns, which can be suitable for cells of various diameters that have been discovered. For example, the pore size of the filter 23 can be 2 microns, 10 microns, 40 microns, 70 microns, 100 microns, 150 microns, 190 microns, etc., among which 40 microns or 70 microns are commonly used pore sizes of the filter 23.

[0043] Optionally, the height of the flow guide 22 is 1 / 5 to 3 / 5 of the depth of the collection hole 11, so that the cell filter 2 can accommodate enough suspension, while preventing the flow guide 22 from being too close to the bottom of the collection hole 11, resulting in the suspension not being able to pass through the filter 23 smoothly. Exemplarily, the height of the flow guide 22 can be 1 / 5, 2 / 5, 1 / 2 or 3 / 5 of the depth of the collection hole 11, etc.

[0044] This embodiment also provides a cell analysis device, including a cell analysis instrument and the above-mentioned cell filtering device, the cell filtering device is configured to filter out cells or cell clusters larger than a preset diameter in the suspension to obtain target cells, and the cell analysis instrument is used to obtain cell parameters of the target cells. By applying the above-mentioned cell filtering device, the cell analysis instrument is prevented from being blocked by cells or cell clusters larger than a preset diameter, and the target cells obtained after filtering through the filter 23 are dispersed, which can improve the accuracy of the cell parameters obtained by the cell analysis instrument. The operator can flexibly select cell filter screens 2 of different specifications and quantities according to usage requirements, thereby improving the flexibility and versatility of the cell analysis instrument, reducing waste, and reducing experimental costs.

[0045] Among them, cell parameters include but are not limited to cell size, cell granularity, cell surface area, nuclear-cytoplasmic ratio, DNA content and cell cycle, RNA content, protein content, cell activity, intracellular cytokines, enzyme activity, etc., which are not limited here.

[0046] Obviously, the above embodiments of the present invention are merely examples for the purpose of clearly illustrating the present invention, and are not intended to limit the implementation methods of the present invention. For those skilled in the art, various obvious changes, readjustments and substitutions can be made without departing from the scope of protection of the present invention. It is not necessary and impossible to list all implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the scope of protection of the claims of the present invention.

Claims

1. A cell filtration device, characterized in that: include: A microplate (1), wherein a plurality of placement areas are provided on the microplate (1), and each of the placement areas is provided with a plurality of collection holes (11) arranged in an array; A cell filter screen (2), each placement area corresponds to a cell filter screen (2), the cell filter screen (2) comprises a filter screen body (21), a filter screen (23) and a plurality of flow guide parts (22), each of the flow guide parts (22) is provided with a through flow guide hole (221), the filter screen body (21) is provided with a liquid collecting hole (211) corresponding to the collection hole (11) in the corresponding placement area, each of the liquid collecting holes (211) is connected to a flow guide part (22), and the liquid collecting hole (211) is arranged opposite to the flow guide hole (221) of the corresponding flow guide part (22), each of the flow guide parts (22) can be inserted into the corresponding collection hole (11), and each of the flow guide holes (221) is provided with the filter screen (23).

2. The cell filtration device according to claim 1, characterized in that Each of the filter screen bodies (21) and the plurality of flow guide portions (22) thereon are an integrally formed structure.

3. The cell filtration device according to claim 1, characterized in that The liquid collecting holes (211) on each of the filter screen bodies (21) are arranged in an M×N array, wherein 1≤M≤12, and 1≤N≤8.

4. The cell filtration device according to claim 1, characterized in that A guide slope is provided at one end of the flow guide portion (22) away from the filter screen body (21), and the guide slope gradually inclines toward the flow guide hole (221) in a direction away from the filter screen body (21).

5. The cell filtration device according to claim 1, characterized in that The filter screen (23) is bonded or hot-pressed to the flow guide portion (22).

6. The cell filtration device according to any one of claims 1 to 5, characterized in that: The filter screen (23) is made of nylon material.

7. The cell filtration device according to any one of claims 1 to 5, characterized in that: The filter screen body (21) is made of polyester material; and / or the flow guide portion (22) is made of polyester material.

8. The cell filtration device according to any one of claims 1 to 5, characterized in that: The pore size of the filter (23) is 0.1 to 200 microns.

9. The cell filtration device according to any one of claims 1 to 5, characterized in that: The height of the guide portion (22) is 1 / 5 to 3 / 5 of the depth of the collecting hole (11).

10. A cell analysis device, characterized in that: The invention comprises a cell analysis instrument and a cell filtration device as claimed in any one of claims 1 to 9, wherein the cell filtration device is configured to filter out cells or cell agglomerates larger than a preset diameter in a suspension to obtain target cells, and the cell analysis instrument is used to obtain cell parameters of the target cells.

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