Chromatographic empty column tube for inhibiting generation of bubbles

Abstract

The utility model provides a chromatography empty column tube capable of inhibiting generation of bubbles, which relates to the technical field of medical equipment and comprises a connecting hose and a molecular sieve joint connected with the connecting hose, the top end of the connecting hose is connected with the molecular sieve joint, the top end of the molecular sieve joint is sleeved with a joint connector, and the top end of the joint connector is provided with a threaded joint. A piston pipe is connected to the top end of the threaded connector, and a piston main body is arranged in the piston pipe, so that bubbles generated in the disassembly process of the purification instrument connected with the outside by the connecting hose can be pushed and removed by matching the piston main body with the piston pipe, structures such as a pressure valve do not need to be independently arranged, and a self-made protection device is additionally arranged through the self structure; during installation, a certain pressure can be manually applied, so that liquid at the front end of the connecting hose cannot flow back into the chromatographic column due to gravity factors, bubbles at the front end of the connecting line are avoided, liquid-liquid connection is easier to realize, and connection of molecular sieves is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, in particular to a chromatography empty column tube for inhibiting the generation of bubbles. Background Art

[0002] In the existing chromatography empty column tube connection technology, when the connecting hose is connected or disconnected from the external purifier, bubbles are often generated at the front end of the connecting hose due to gas mixing or liquid reflux during operation. These bubbles may enter the chromatography column along with the liquid, affecting the effect and accuracy of chromatographic separation. In order to remove these bubbles, the traditional method may require the installation of additional pressure valves or other structures, which not only increases the complexity and cost of the system, but also may affect the stability and reliability of the system.

[0003] In the prior art, molecular sieve chromatography columns are connected end to end, which results in bubbles being easily generated when connected to the chromatography instrument AKTA. Generally, a separate pressure valve is required for adjustment, or manual adjustment is required. Bubbles are difficult to discharge and control. Utility Model Content

[0004] The utility model aims to solve the defect in the prior art that bubbles in the empty chromatography column tube cannot be discharged through its own structure, and proposes an empty chromatography column tube for inhibiting the generation of bubbles.

[0005] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a chromatography empty column tube for suppressing the generation of bubbles, comprising a connecting hose and a molecular sieve joint connected to the connecting hose, the top end of the connecting hose is connected to a molecular sieve joint, the top end of the molecular sieve joint is sleeved with a joint connector, the top end of the joint connector is provided with a threaded joint, the top end of the threaded joint is connected to a piston tube, and a piston body is provided inside the piston tube.

[0006] Preferably, one end of the molecular sieve joint and one end of the threaded joint are both sleeved with the inner wall of the end of the joint connector.

[0007] Preferably, the vertical center line of the molecular sieve joint, the vertical center line of the joint connector and the vertical center line of the threaded joint all coincide with each other.

[0008] Preferably, the outer wall of one end of the piston body abuts against the inner wall of the piston tube, and the end of the piston body abuts against the inner wall of the piston tube and is sealed with rubber.

[0009] Preferably, one end of the threaded joint away from the joint connector is threadedly sealed to the end of the piston tube.

[0010] Preferably, the two ends of the joint connector are of the same size, and the two ends of the threaded joint and the molecular sieve joint are of different sizes.

[0011] Preferably, the connection position between the connecting hose and the molecular sieve connector is sealed, and the outer edge of the connector is deburred.

[0012] Beneficial Effects

[0013] In the utility model, a piston tube is connected to the end of a connecting hose through a molecular sieve joint, a joint connector and a threaded joint to cooperate with a piston body for use, so that bubbles generated during the disassembly process of connecting the connecting hose to an external purifier can be pushed and removed by the piston body and the piston tube, without the need to separately set up a pressure valve and other structures. A self-made protective device is added through its own structure, and a certain pressure can be manually applied during installation, so that the liquid at the front end of the connecting hose will not flow back into the chromatography column due to gravity factors, thereby avoiding the generation of bubbles at the front end of the connecting line, making it easier to achieve liquid-liquid connection, and facilitating the connection of the molecular sieve. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a three-dimensional structural diagram of the utility model;

[0015] Figure 2 It is an axonometric drawing of the utility model;

[0016] Figure 3 It is a front cross-sectional view of the utility model;

[0017] Figure 4 It is a front view of the utility model.

[0018] Legend:

[0019] 1. Molecular sieve connector; 2. Connecting hose; 3. Connector connector; 4. Threaded connector; 5. Piston tube; 6. Piston body. DETAILED DESCRIPTION

[0020] In order to make the technical means, creative features, objectives and effects of the present invention easy to understand, the present invention is further described below in conjunction with specific embodiments and drawings, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the embodiments in the implementation mode, other embodiments obtained by those skilled in the art without creative work are all within the protection scope of the present invention.

[0021] The specific embodiments of the present utility model are described below in conjunction with the accompanying drawings. Specific embodiment one:

[0023] Reference Figure 1-4, a chromatography empty column tube for inhibiting the generation of bubbles, comprises a connecting hose 2 and a molecular sieve joint 1 connected to the connecting hose 2, the top of the connecting hose 2 is connected with a molecular sieve joint 1, the top of the molecular sieve joint 1 is sleeved with a joint connector 3, the top of the joint connector 3 is provided with a threaded joint 4, the top of the threaded joint 4 is connected with a piston tube 5, the piston tube 5 is provided with a piston body 6, an appropriate amount of water is added to the piston tube 5, and a certain back pressure can be provided by pushing the piston body 6 to prevent the pipeline from generating liquid empty phenomenon at the upper end joint, the piston tube 5 is connected to the piston body 6 at the end of the connecting hose 2 through the molecular sieve joint 1, the joint connector 3 and the threaded joint 4 for use, so that the bubbles generated during the disassembly process of the connecting hose 2 when connecting to the external purification instrument can be pushed and removed by the piston body 6 and the piston tube 5, without the need to separately set up structures such as pressure valves, a self-made protective device is added through its own structure, and a certain pressure can be manually applied during installation, so that the liquid at the front end of the connecting hose 2 will not flow back into the chromatography column due to gravity factors, thereby avoiding the generation of bubbles at the front end of the connecting line, making it easier to achieve liquid-liquid connection, and facilitating the connection of molecular sieves.

[0024] Other limiting structures of the entire device are as follows: one end of the molecular sieve joint 1 and one end of the threaded joint 4 are both sleeved with the inner wall of the end of the joint connector 3 to maintain the stability of the connection and prevent it from falling off; the vertical center lines of the molecular sieve joint 1, the joint connector 3 and the threaded joint 4 are all coincident with each other, so that a straight path is formed between the connected pipes, which is convenient for liquid diversion; the outer wall of one end of the piston body 6 is against the inner wall of the piston tube 5, and the end of the piston body 6 is against the inner wall of the piston tube 5 and is treated with rubber sealing to maintain a sealed environment, which is convenient for removing bubbles by pushing or pulling; the end of the threaded joint 4 away from the joint connector 3 is threadedly sealed with the end of the piston tube 5; the two ends of the joint connector 3 are the same size, and the sizes of the threaded joint 4 and the molecular sieve joint 1 are different; the connection hose 2 is sealed at the connection position with the molecular sieve joint 1, and the outer edge of the joint connector 3 is deburred. Specific embodiment 2:

[0026] Reference Figure 1-4 Due to the problem of the molecular sieve column, bubbles are more likely to be generated when connected to the purifier and during the disassembly process. Improper handling will affect the separation effect of the molecular sieve. If bubbles enter, at least you can use AKTA to remove the bubbles online. When there is more air intake, you need to refill it, which is time-consuming and seriously affects the normal progress of the experiment. This device has a self-made protective device added to it. During installation, a certain pressure can be manually applied so that the liquid at the front end will not flow back into the chromatography column due to gravity, thereby avoiding the generation of bubbles at the front end of the connecting line, making it easier to achieve liquid-liquid connection and facilitating the connection of the molecular sieve.

[0027] In practical application, this application can be completed by using laboratory equipment, syringes, and adapters. It is easy to use, simple in structure, low in cost, and easy to operate. This device is relatively convenient and made of simple materials. Laboratories generally have it, and there is no need to purchase it additionally. The connection problem of the chromatography column can be easily solved, and there is no need to worry about introducing bubbles during installation, which will affect the normal use of the molecular sieve. Specific embodiment three:

[0029] Reference Figure 1-4 When the entire device is stored and used in the main trunk, ensure that the connecting hose 2, molecular sieve joint 1, joint connector 3, threaded joint 4, piston tube 5 and piston body 6 and other components are intact, clean and dry. During the installation process, manually give a certain pressure to the connecting hose 2 to ensure that the liquid at the front end of the hose will not flow back into the chromatography column due to gravity. Check whether each connection is firm to avoid leakage and bubble generation. When it is necessary to disassemble the external purifier, push the piston body 6 with the piston tube 5 to push the bubbles that may be generated in the connecting hose 2 to the outside to prevent bubbles from entering the chromatography column. During the operation, pay attention to maintaining appropriate pressure and speed to ensure the effective removal of bubbles. Specific embodiment four:

[0031] Reference Figure 1-4 When the whole device is actually used, the method and principle of using the chromatography column can refer to the existing technology. The specific steps are as follows:

[0032] Choose the right column: Choose the right column size and filler type based on the experimental requirements and the properties of the sample to be separated;

[0033] Cleaning the chromatography column: Use appropriate solvents such as ethanol, methanol, water, etc. to clean the chromatography column to ensure that there are no impurities and residues in the column;

[0034] Filling: The selected filler such as silica gel, alumina, activated carbon, etc. is uniformly filled into the chromatography column and compacted to eliminate bubbles. The bubbles are eliminated by the cooperation of the piston body 6 and the piston tube 5;

[0035] Install the cleaned chromatography column on the chromatograph, making sure the connection is tight and leak-free;

[0036] Check whether the outlet of the chromatography column is unobstructed so that the liquid can flow out smoothly;

[0037] Loading: Slowly add the sample solution to be separated into the chromatography column to ensure that the sample is evenly distributed on the filler;

[0038] Elution: Use an appropriate eluent, usually a mobile phase, to pass through the chromatography column at a constant flow rate, so that different components in the sample are adsorbed, desorbed and separated on the filler;

[0039] According to experimental requirements, use a collector or automatic collection system to collect different components flowing out of the chromatography column, and pay attention to the color and concentration changes of the effluent to determine the collection time and range of different components.

[0040] After the experiment, use an appropriate solvent to clean the chromatography column to remove residual samples and eluent, and store the cleaned chromatography column in a dry, cool place, avoiding direct sunlight and high temperature.

[0041] During the experiment, the chromatography column should be kept covered with liquid at all times to avoid dry column phenomenon that may cause the filler to agglomerate or break. During the elution process, the flow rate should be kept constant to avoid too fast flow rate that may lead to poor separation effect or too slow flow rate that may lead to too long separation time.

[0042] Choose the right eluent: Choose the right eluent based on the sample properties and separation requirements to optimize the separation effect;

[0043] Record experimental data: During the experiment, the experimental conditions, operating steps, experimental results and other data should be recorded in detail for subsequent analysis and summary.

[0044] In summary:

[0045] The piston tube 5 is connected to the piston body 6 at the end of the connecting hose 2 through the molecular sieve joint 1, the joint connector 3 and the threaded joint 4 for use, so that bubbles generated during the disassembly process of the connecting hose 2 connecting to the external purifier can be pushed and removed by the piston body 6 and the piston tube 5. There is no need to set up a pressure valve and other structures separately. A self-made protective device is added through its own structure. A certain pressure can be manually applied during installation, so that the liquid at the front end of the connecting hose 2 will not flow back into the chromatography column due to gravity factors, thereby avoiding the generation of bubbles at the front end of the connecting line, making it easier to achieve liquid-liquid connection, and facilitating the connection of the molecular sieve.

[0046] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0047] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the utility model and are not used to limit the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection of the utility model is defined by the attached claims and their equivalents.

Claims

1. A chromatography empty column tube for suppressing bubble generation, comprising a connecting hose (2) and a molecular sieve connector (1) connected to the connecting hose (2), characterized in that: The top end of the connecting hose (2) is connected to a molecular sieve joint (1), the top end of the molecular sieve joint (1) is sleeved with a joint connector (3), the top end of the joint connector (3) is provided with a threaded joint (4), the top end of the threaded joint (4) is connected to a piston tube (5), and a piston body (6) is provided inside the piston tube (5).

2. The chromatography empty column tube for suppressing bubble generation according to claim 1, characterized in that: One end of the molecular sieve joint (1) and one end of the threaded joint (4) are both sleeved with the inner wall of the end of the joint connector (3).

3. The chromatography empty column tube for suppressing bubble generation according to claim 1, characterized in that: The vertical center line of the molecular sieve joint (1), the vertical center line of the joint connector (3) and the vertical center line of the threaded joint (4) all coincide with each other.

4. The chromatography empty column tube for suppressing bubble generation according to claim 1, characterized in that: The outer wall of one end of the piston body (6) abuts against the inner wall of the piston tube (5), and the end of the piston body (6) abuts against the inner wall of the piston tube (5) and is sealed with rubber.

5. The chromatography empty column tube for suppressing bubble generation according to claim 1, characterized in that: One end of the threaded joint (4) away from the joint connector (3) is threadedly sealedly connected to the end of the piston tube (5).

6. The chromatography empty column tube for suppressing bubble generation according to claim 1, characterized in that: The two ends of the joint connector (3) are of the same size, while the two ends of the threaded joint (4) and the molecular sieve joint (1) are of different sizes.

7. The chromatography empty column tube for suppressing bubble generation according to claim 1, characterized in that: The connection position between the connecting hose (2) and the molecular sieve joint (1) is sealed, and the outer edge of the joint connector (3) is deburred.

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