A new type of rat fixation device for assisting spinal cord percussion modeling
By designing a rat fixing device including positioning plate, head strap, tail strap and body limiting assembly, the problems of inaccurate positioning, unsolid fixing, poor adaptability and high cost in the prior art are solved, and efficient and accurate construction of spinal cord injury model is achieved.
Patent Information
- Application Number
- CN202420642974.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-29
- Publication Date
- 2025-04-04
- Estimated Expiration
- 2034-03-29
AI Technical Summary
The existing experimental mouse fixation devices have problems such as inaccurate positioning, unsolid fixation, poor adaptability and high cost in the construction of spinal cord injury models, which affect the success rate and efficiency of modeling.
A rat fixing device including a positioning plate, a head strap, a tail strap and a body limiting assembly was designed. Through the combination of sliders and bolts, the precise positioning and firm fixation of the experimental mice is achieved, and through the design of through holes and tight ropes, it is adapted to experimental mice of different sizes.
It improves the position accuracy and fixing firmness of spinal cord attack molding, enhances the success rate and efficiency of molding, and reduces production costs. It is suitable for experimental mice of different strains and sizes.
Smart Images
Figure CN222708452U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to an experimental device, in particular to a device which is convenient for positioning and fixing and is used to assist in building an animal spinal cord injury model. Background Art
[0002] Spinal cord injury is caused by displacement of vertebrae or protrusion of bone fragments into the spinal canal, resulting in varying degrees of damage to the spinal cord or cauda equina. It is a central nervous system injury with a high disability and mortality rate, which can lead to a series of complications such as varying degrees of limb paralysis, loss of sensation or autonomic function. There is currently a lack of clear and effective means for the treatment of spinal cord injury. The reason is that the pathophysiological changes after spinal cord injury are very complex, and there is currently no comprehensive and in-depth understanding and knowledge of them. Establishing an animal model with high reliability and good repeatability is a standard for studying the mechanism of spinal cord injury, observing pathophysiological changes after injury, and exploring the degree of spinal cord injury. It is also a basic prerequisite for exploring drug and stem cell treatment methods.
[0003] The construction of spinal cord injury models usually uses mice, rats, rabbits and other animals for experiments. The animals need to be anesthetized and fixed in a prone position, the back hair removed and thoroughly disinfected, a 2 cm long longitudinal incision made in the center of the thoracic spine, the T10 vertebral plate carefully cut off and the spinal cord exposed, and then different measures (such as impactor, clamp compression, etc.) are taken to construct different types of spinal cord injuries. In order to assist the accuracy of the construction of different spinal cord injury models, a laboratory mouse fixation device is needed to fix the laboratory mouse, but the existing laboratory mouse fixation device has the following shortcomings:
[0004] 1. Since the objects being struck are usually small mammals such as mice, the modeling site is often placed slightly above the animal's spine, leaving a very small size that is not conducive to naked eye observation. There is a lack of a reference system to ensure that the field of view of the spine exposed during surgery remains vertical, making it difficult to determine the center of the strike; 2. There is a lack of reliable fixation. Due to the impact of some striking devices, the modeled animals may be displaced, and the modeling position is inaccurate, affecting the success rate of the modeling; 3. Due to the physical differences of different experimental animals, the same device was previously difficult to adapt to the fixation of experimental animals of different strains and sizes, affecting the efficiency and success rate of the modeling; 4. The structure is complex, the production cost is high, and it is difficult to popularize in basic scientific research. Utility Model Content
[0005] Aiming at the defects in the prior art, the visual reference system is increased, the fixation of the experimental mice is strengthened, and the success rate of modeling is improved. The utility model provides a new rat fixation device for assisting spinal cord percussion modeling, including a positioning plate, a head strap, a tail strap and a body limiting component;
[0006] The body limiting assembly includes a left slide rail and a right slide rail, a left middle portion of the positioning plate is provided with a transverse mounting groove adapted to the left slide rail, and a right middle portion of the positioning plate is provided with another transverse mounting groove adapted to the right slide rail; sliders are provided on the upper side of the left slide rail and the upper side of the right slide rail, and the left slide rail and the right slide rail are respectively installed in the corresponding transverse mounting grooves, and the two sliders are not lower than the upper side of the positioning plate, thereby forming a space for clamping the body of the experimental mouse between the two sliders;
[0007] The positioning plate is evenly covered with longitudinal through holes, and the head strap and the tail strap are respectively provided with tightening ropes. The head strap and the tail strap are respectively put on the head and the tail of the experimental mouse, and the tightening ropes are passed through the corresponding through holes and then tightened, thereby fixing the head and the tail of the experimental mouse.
[0008] The beneficial effects of the utility model are:
[0009] 1. First, place the experimental mouse between the two sliders on the positioning plate. Move the two sliders toward the middle to clamp the mouse's body. Then, the head and tail of the experimental mouse are trapped with the head strap and tail strap respectively, and then the head strap and tail strap are fixed to the corresponding position of the positioning plate with a tightening rope. The head-neck-trunk-tail of the experimental mouse are located on the same vertical line, ensuring the accurate position during spinal cord impact modeling, which is conducive to improving the success rate of experimental modeling.
[0010] 2. The positioning plate is covered with through holes. According to the body size of the experimental mouse, the tightening rope is tightened through the holes in the appropriate position. Similarly, the slider is also fixed in the appropriate position, so it is suitable for experimental mice of different sizes and has strong versatility.
[0011] Preferably, it also includes locking ropes, and there are four locking ropes. After passing through corresponding through holes, the four locking ropes fix the limbs of the experimental mouse to the upper left, lower left, lower right and upper right of the positioning plate respectively. One end of the locking rope is tied to the toes or legs of the experimental mouse, and the other end is fixed after passing through the through hole. The fixing method is the same as that of the tightening rope, and the appropriate through hole can be selected for fixing according to the size of the experimental mouse.
[0012] Preferably, the head strap and the tail strap are both provided with a Japanese-shaped buckle for adjusting the tightness. The Japanese-shaped buckle is convenient for adjusting the tightness to prevent the animal from suffocating, blood flow to the limbs, etc.
[0013] Preferably, a tightening rope is provided on the left side and the right side of the head strap, and similarly, a tightening rope is provided on the left side and the right side of the tail strap.
[0014] Preferably, the positioning plate is provided with horizontal and vertical reference lines, and all the reference lines pass through the center of the corresponding through hole. The horizontal and vertical edges of the positioning plate are provided with scale numbers corresponding to the reference lines. The reference lines assist in determining whether the head-neck-trunk-tail are located on the same vertical line, and the scale numbers can quickly determine the tightening position of the tightening rope and the locking rope, avoiding misalignment of the tightening position and affecting the tightening effect.
[0015] Preferably, each slider is provided with a bolt for locking. Each slider is provided with a longitudinal threaded hole, and each bolt is threadedly connected to the corresponding threaded hole, and the slider is locked by turning the thread downward and tightening the lower end of the bolt against the corresponding left or right slide rail.
[0016] Preferably, a baffle plate in contact with the experimental mouse is provided on the side of each slider, which increases the contact area with the experimental mouse and improves the clamping effect. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] In order to more clearly illustrate the specific implementation of the utility model or the technical solution in the prior art, the following is a brief introduction to the drawings required for the specific implementation or the prior art description. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, each element or part is not necessarily drawn according to the actual scale.
[0018] Figure 1 It is a structural schematic diagram of this embodiment.
[0019] In the accompanying drawings, there are positioning plate 1, head strap 2, tail strap 3, through hole 4, reference line 5, scale number 6, left slide rail 7, right slide rail 8, slider 9, mounting slot 10, bolt 11, Japanese buckle 12, tightening rope 13, locking rope 14, lighting lamp 15, splint base 16, and experimental mouse 17. DETAILED DESCRIPTION
[0020] The following embodiments of the technical solution of the utility model are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the utility model, and are therefore only used as examples, and cannot be used to limit the protection scope of the utility model.
[0021] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application should have the common meanings understood by technicians in the field to which the utility model belongs.
[0022] like Figure 1As shown, this embodiment provides a novel rat fixation device for assisting spinal cord impact modeling, including a positioning plate 1, a head strap 2, a tail strap 3 and a body limiting assembly. The positioning plate 1 is evenly covered with longitudinal through holes 4, and the positioning plate 1 is provided with transverse and longitudinal reference lines 5, and all reference lines 5 pass through the center of the corresponding through holes 4. In addition, scale numbers 6 corresponding to the reference lines 5 are provided on the transverse and longitudinal edges of the positioning plate 1. The scale numbers 6 increase in sequence according to the reference lines 5 on the transverse edges. Similarly, the reference lines 5 on the longitudinal edges also have scale numbers 6 that increase in sequence.
[0023] The specific structure of the body limiting assembly in this embodiment is as follows:
[0024] The body limiting assembly includes a left slide rail 7 and a right slide rail 8. A transverse mounting groove 10 adapted to the left slide rail 7 is provided on the left middle portion of the positioning plate 1, and another transverse mounting groove 10 adapted to the right slide rail 8 is provided on the right middle portion of the positioning plate 1. Slide blocks 9 are provided on the upper portion of the left slide rail 7 and the upper portion of the right slide rail 8. The left slide rail 7 and the right slide rail 8 are respectively installed in the corresponding transverse mounting grooves 10, and the two slide blocks 9 are not lower than the upper portion of the positioning plate 1, thereby forming a space between the two slide blocks 9 for clamping the body of the experimental mouse 17. The left slide rail 7 and the right slide rail 8 are not higher than the upper surface of the positioning plate 1, and do not affect the placement of the experimental mouse 17. At the same time, the two slide blocks 9 can clamp the experimental mouse 17. In order to maintain the clamping effect of the slide blocks 9, the slide blocks 9 are provided with bolts 11 for locking. Each slider 9 is provided with a longitudinal threaded hole, and each bolt 11 is connected to the corresponding threaded hole by threading. The thread is turned downward and the lower end of the bolt 11 is pressed against the corresponding left slide rail 7 or right slide rail 8, thereby locking the slider 9. In addition, a baffle is provided on the side of each slider 9 to contact the experimental mouse 17. The baffle increases the contact area with the experimental mouse 17, further improving the clamping effect.
[0025] In this example, the head strap 2 and the tail strap 3 are respectively put on the head and tail of the experimental mouse 17, and the head strap 2 and the tail strap 3 are both provided with a Japanese-shaped buckle 12 for adjusting the tightness. The Japanese-shaped buckle 12 is convenient for adjusting the tightness to prevent the animal from suffocating, blood flow to the limbs, etc. The head strap 2 and the tail strap 3 are both provided with a tightening rope 13. The head strap 2 and the tail strap 3 are respectively put on the head and tail of the experimental mouse 17, and the tightening rope 13 is passed through the corresponding through hole 4 and then tightened, thereby fixing the head and tail of the experimental mouse 17. Specifically, a tightening rope 13 is respectively provided on the left and right sides of the head strap 2, and similarly, a tightening rope 13 is respectively provided on the left and right sides of the tail strap 3.
[0026] The limbs of the experimental mouse 17 also need to be fixed, so this embodiment also includes locking ropes 14, and the locking ropes 14 are provided with four. After the four locking ropes 14 pass through the corresponding through holes 4, the limbs of the experimental mouse 17 are fixed to the upper left, lower left, lower right and upper right of the positioning plate 1. One end of the locking rope 14 is tied tightly to the toes or legs of the experimental mouse 17, and the other end is fixed after passing through the through hole 4, which is the same as the fixing method of the tightening rope 13, and the appropriate through hole 4 can be selected for fixing according to the size of the experimental mouse 17.
[0027] The head of the experimental mouse 17 is fixed by a head strap 2 and a tightening rope 13, the body is clamped between two sliders 9, the limbs are fixed by four locking ropes 14 one by one, and finally the tail is fixed by a tail strap 3 and a tightening rope 13. The entire experimental mouse 17 is completely fixed, and the fixing effect is significantly improved compared to the existing fixing device. Moreover, it can be fixed at any position on the positioning plate 1, and can lie prone or supine, and is also suitable for other experiments. The head-neck-trunk-tail of the experimental mouse 17 is located on the same vertical line, which ensures the accurate position during spinal cord percussion modeling, which is conducive to improving the success rate of experimental modeling.
[0028] In addition, the positioning plate 1 in this embodiment is provided with a lighting lamp 15 with a clamping base 16, the clamping base 16 is clamped on the edge of the positioning plate 1, and the lighting lamp 15 is provided with a universal tube that can be bent at will, so as to facilitate the adjustment of the position of the lighting lamp 15.
[0029] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the utility model, and they should all be included in the scope of the claims and specification of the utility model.
Claims
1. A novel rat fixation device for assisting spinal cord percussion modeling, characterized in that: It includes a positioning plate, a head strap, a tail strap and a body limiting assembly; The body limiting assembly includes a left slide rail and a right slide rail, a horizontal mounting groove adapted to the left slide rail is provided on the left middle portion of the positioning plate, and another horizontal mounting groove adapted to the right slide rail is provided on the right middle portion of the positioning plate; sliders are provided on the upper portion of the left slide rail and the upper portion of the right slide rail, and the left slide rail and the right slide rail are respectively installed in the corresponding horizontal mounting grooves, and the two sliders are not lower than the upper portion of the positioning plate, thereby forming a space for clamping the body of the experimental mouse between the two sliders; The positioning plate is evenly covered with longitudinal through holes, and the head strap and the tail strap are respectively provided with tightening ropes. The head strap and the tail strap are respectively put on the head and the tail of the experimental mouse, and the tightening ropes are passed through the corresponding through holes and then tightened, thereby fixing the head and the tail of the experimental mouse.
2. A novel rat fixation device for assisting spinal cord percussion modeling according to claim 1, characterized in that: It also includes four locking ropes, which pass through corresponding through holes to fix the four limbs of the experimental mouse to the upper left, lower left, lower right and upper right of the positioning plate respectively.
3. A novel rat fixation device for assisting spinal cord percussion modeling according to claim 1, characterized in that: The head strap and the tail strap are both provided with Japanese-style buckles for adjusting tightness.
4. A novel rat fixation device for assisting spinal cord percussion modeling according to claim 1, characterized in that: A tightening rope is respectively provided on the left side and the right side of the head strap, and a tightening rope is respectively provided on the left side and the right side of the tail strap.
5. The novel rat fixation device for assisting spinal cord percussion modeling according to claim 1 is characterized in that: The positioning plate is provided with a lighting lamp with a clamping plate base.
6. A novel rat fixation device for assisting spinal cord percussion modeling according to claim 1, characterized in that: The positioning plate is provided with transverse and longitudinal reference lines, and all the reference lines pass through the centers of the corresponding through holes.
7. A novel rat fixation device for assisting spinal cord percussion modeling according to claim 2, characterized in that: The horizontal edge and the vertical edge of the positioning plate are both provided with scale numbers corresponding to the reference lines.
8. The novel rat fixation device for assisting spinal cord percussion modeling according to claim 1 is characterized in that: Each slide block is provided with a bolt for locking.
9. A novel rat fixation device for assisting spinal cord percussion modeling according to claim 8, characterized in that: Each slider is provided with a longitudinal threaded hole, and each bolt is connected to the corresponding threaded hole through a thread. The slider is locked by turning the thread downward and tightening the lower end of the bolt against the corresponding left slide rail or right slide rail.
10. The novel rat fixation device for assisting spinal cord percussion modeling according to claim 1 is characterized in that: Each slide is provided with a baffle on the side for contact with the experimental mouse.