Microfluidic device and microfluidic equipment comprising same

Abstract

The utility model provides a microfluidic device and microfluidic equipment comprising the same. The microfluidic device comprises a first chip and a second chip which are stacked and can slide relative to each other, wherein the first chip and the second chip are made of transparent materials; the first surface of the first chip and the second surface of the second chip are attached to each other, the first surface of the first chip is provided with a micro-channel for microorganism interaction, and the second surface of the second chip is provided with a plurality of pre-culture micro-grooves which are arranged at intervals and are used for microorganism pre-culture; the first surface of the first chip is also provided with a first liquid inlet channel and a first liquid outlet channel which can be respectively communicated with the pre-culture micro groove, and the second surface of the second chip is also provided with a second liquid inlet channel and a second liquid outlet channel which can be respectively communicated with the micro channel. According to the utility model, the position change can be freely carried out by utilizing the chip, and the interaction time and the contact area of microorganisms can be controlled, so that the precise control on parameters is realized.

Description

Technical Field

[0001] The utility model relates to a microfluidic device and microfluidic equipment comprising the same. Background Art

[0002] Microorganisms play an important role in nature and industrial environments, and their interactions have a significant impact on ecosystem balance, environmental remediation, and industrial biotechnology. Traditional methods for studying microbial interactions mainly rely on solid or liquid culture media to observe and analyze the relationships between different microorganisms through plate streaking, co-culture, and liquid culture.

[0003] However, these methods have problems such as complex operation, long experimental cycle, and inability to accurately control the contact area and contact time of microorganisms, which limits the refined study of microbial interactions. Microfluidic chip technology has the advantages of high throughput, low sample consumption and precise control. However, most existing microfluidic chips are fixed structures, making it difficult to achieve real-time monitoring and control of dynamic changes in the process of microbial interactions. Utility Model Content

[0004] The technical problem to be solved by the utility model is to overcome the defect in the prior art that the dynamic changes in the process of microbial interaction cannot be monitored and controlled in real time, and to provide a microfluidic device and a microfluidic equipment containing the same.

[0005] The utility model solves the above technical problems through the following technical solutions:

[0006] A microfluidic device, characterized in that it comprises: a first chip and a second chip which are stacked and relatively slidably arranged, wherein the first chip and the second chip are both made of transparent materials;

[0007] The first surface of the first chip and the second surface of the second chip are bonded to each other, the first surface of the first chip is provided with a microchannel for microbial interaction, and the second surface of the second chip is provided with a plurality of pre-culture micro-grooves for pre-culture of microorganisms arranged at intervals; the first surface of the first chip is also provided with a first liquid inlet channel and a first liquid outlet channel respectively connected to the pre-culture micro-grooves, and the second surface of the second chip is also provided with a second liquid inlet channel and a second liquid outlet channel respectively connected to the microchannel;

[0008] Wherein, when the first chip and the second chip are in the first working position, the microchannel and the plurality of pre-culture micro-grooves do not overlap in the thickness direction, and the microchannel and the plurality of pre-culture micro-grooves are not connected; the microchannel is connected with the second liquid inlet channel and the second liquid outlet channel, and the plurality of pre-culture micro-grooves are connected with the corresponding first liquid inlet channel and first liquid outlet channel;

[0009] When the first chip and the second chip are in the second working position, the microchannel at least partially overlaps with the plurality of pre-culture micro-grooves along the thickness direction, and the microchannel is connected to the plurality of pre-culture micro-grooves; the microchannel is disconnected from the second liquid inlet channel and the second liquid outlet channel, and the plurality of pre-culture micro-grooves are disconnected from the corresponding first liquid inlet channel and first liquid outlet channel.

[0010] In this technical solution, by setting up a first chip and a second chip that are stacked and relatively slidable, and the specific structure thereon, when conducting research on microbial interactions, the chip can be used to freely change its position, and the time and contact area of ​​microbial interaction can be controlled, thereby achieving precise control of parameters; before the microorganisms enter the microchannel for interaction, the flow channel formed by the sliding chip can be connected or disconnected to achieve the distribution of microorganisms in the pre-culture micro-grooves, and then air bath culture is performed in the incubator, and the microorganisms can grow rapidly in the pre-culture micro-grooves, greatly shortening the experimental cycle. In addition, by setting the materials of the first chip and the second chip to be transparent materials, they have good light transmittance, so as to monitor the growth status, movement trajectory and interaction status of the microorganisms in real time and capture multi-dimensional information.

[0011] Preferably, the microfluidic device further includes: a first fixing module and a second fixing module, the first chip is fixed on the first fixing module, the second chip is fixed on the second fixing module, and the materials of the first fixing module and the second fixing module are both transparent materials.

[0012] In the present technical solution, by fixing the first chip on the first fixing module and the second chip on the second fixing module, the relative position of the first chip and the second chip can be easily determined, and the relative sliding of the first chip and the second chip can be easily driven. At the same time, the sliding position of the first chip and the second chip can be limited to ensure that the sliding process is smooth and the positioning is accurate.

[0013] Preferably, the first fixing module is provided with a first accommodating cavity, a non-zero spacing is provided between a side wall of the first accommodating cavity and an outer side wall of the first fixing module, the first chip is embedded in the first accommodating cavity, and the first accommodating cavity is a groove or a through hole; and / or,

[0014] The second fixing module is provided with a second accommodating cavity, a non-zero spacing is provided between the side wall of the second accommodating cavity and the outer side wall of the second fixing module, the second chip is embedded in the second accommodating cavity, and the second accommodating cavity is a groove or a through hole.

[0015] In this technical solution, by setting the specific structure of the first fixing module, the first chip can be better fixed. By setting the specific structure of the second fixing module, the second chip can be better fixed.

[0016] Preferably, along the thickness direction, a portion of the first fixing module located outside the orthographic projection of the first chip is covered with a first light shielding layer; and / or,

[0017] Along the thickness direction, a portion of the second fixing module located outside the orthographic projection of the second chip is covered with a second light shielding layer.

[0018] In this technical solution, by setting the first shading layer and the second shading layer, the interference of external light sources on the experiment can be reduced to a certain extent, that is, the impact of external light sources on the experimental environment can be reduced to a certain extent, creating a relatively closed and stable imaging condition.

[0019] Preferably, the thickness of the first fixing module is greater than or equal to the thickness of the first chip; and / or,

[0020] The thickness of the second fixing module is greater than or equal to the thickness of the first chip.

[0021] Preferably, the first chip is provided with a first injection hole penetrating along the thickness direction, and the first injection hole is arranged at an end of the first liquid inlet channel away from the first liquid outlet channel; and / or,

[0022] The second chip is provided with a second liquid injection hole penetrating along the thickness direction, and the second liquid injection hole is arranged at an end of the second liquid inlet channel away from the second liquid outlet channel.

[0023] In this technical solution, by setting a first injection hole penetrating the first chip in the thickness direction, liquid can be easily injected into the first liquid inlet channel from the outside. By setting a second injection hole penetrating the second chip in the thickness direction, liquid can be easily injected into the second liquid inlet channel from the outside.

[0024] Preferably, the depths of the first liquid inlet channel and the first liquid outlet channel are both less than the thickness of the first chip, and the depths of the first liquid inlet channel and the first liquid outlet channel are both greater than or equal to 10 microns and less than or equal to 200 microns; and / or,

[0025] The depths of the second liquid inlet channel and the second liquid outlet channel are both less than the thickness of the second chip, and the depths of the second liquid inlet channel and the second liquid outlet channel are both greater than or equal to 10 microns and less than or equal to 200 microns.

[0026] In the present technical solution, the depth range of the first liquid inlet channel, the first liquid outlet channel, the second liquid inlet channel and the second liquid outlet channel is set to ensure that the depth of each channel is not too deep or too shallow. This is because if it is too shallow, it will cause excessive resistance and it will be difficult to pump the liquid into the channel; if it is too deep, there will be a risk that the liquid will not easily fill the channel, resulting in the inability to control the amount, which will affect the experimental results.

[0027] Preferably, the microfluidic device further comprises a lubricating layer, and the lubricating layer is arranged between the first surface of the first chip and the second surface of the second chip.

[0028] In the present technical solution, by setting a lubricating layer between the first surface of the first chip and the second surface of the second chip, the frictional resistance during the relative sliding between the first chip and the second chip can be effectively reduced, making the transition between the first working position and the second working position of the first chip and the second chip smoother; at the same time, by setting a lubricating layer between the first surface of the first chip and the second surface of the second chip, external interference during the culture process can also be prevented.

[0029] Preferably, the thickness of the lubricating layer is less than or equal to 10 microns.

[0030] A microfluidic device is characterized in that it comprises the microfluidic device as described above.

[0031] The positive and progressive effects of the utility model are:

[0032] The utility model sets a first chip and a second chip that are stacked and relatively slidable, and the specific structure thereon. When conducting research on microbial interactions, the chip can be used to freely change its position, and the overlapping time and area of ​​the pre-culture micro-grooves and micro-channels can be adjusted, thereby controlling the time and contact area of ​​microbial interaction, thereby achieving precise control of parameters; before the microorganisms enter the microchannels for interaction, the flow channel formed by the sliding chip can be connected or disconnected to achieve the distribution of the microorganisms in the pre-culture micro-grooves, and then the air bath culture is carried out in the incubator, and the microorganisms can grow rapidly in the pre-culture micro-grooves, greatly shortening the experimental cycle. In addition, by setting the materials of the first chip and the second chip to be transparent materials, they have good light transmission properties, so as to monitor the growth status, movement trajectory and interaction status of the microorganisms in real time and capture multi-dimensional information. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 The figure is a schematic diagram of the three-dimensional structure of a microfluidic device according to a preferred embodiment of the present invention.

[0034] Figure 2This is a schematic diagram of the exploded three-dimensional structure of a microfluidic device according to a preferred embodiment of the utility model.

[0035] Figure 3 It is a schematic diagram of the three-dimensional structure of the first chip and the first fixing module according to a preferred embodiment of the utility model.

[0036] Figure 4 It is a schematic diagram of the three-dimensional structure of the second chip and the second fixing module of a preferred embodiment of the utility model.

[0037] Figure 5 This is a schematic diagram of a three-dimensional structure of a first chip and a second chip located at a first working position according to a preferred embodiment of the utility model.

[0038] Figure 6 This is a schematic diagram of a three-dimensional structure of a first chip and a second chip located at a second working position according to a preferred embodiment of the utility model.

[0039] Description of Reference Numerals

[0040] Microfluidic device 1

[0041] First chip 10

[0042] Microchannel 11

[0043] The first liquid inlet channel 12

[0044] The first liquid outlet channel 13

[0045] First injection hole 14

[0046] The second chip 20

[0047] Pre-culture micro groove 21

[0048] The second liquid inlet channel 22

[0049] The second liquid outlet channel 23

[0050] Second injection hole 24

[0051] Liquid outlet 25

[0052] The first fixing module 30

[0053] The first accommodating chamber 31

[0054] The second fixing module 40

[0055] The second accommodating chamber 41 DETAILED DESCRIPTION

[0056] The embodiments of the utility model are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the utility model, and cannot be understood as limiting the utility model. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the utility model.

[0057] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "circumferential", "radial" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0058] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present utility model, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0059] like Figures 1 to 6 As shown, this embodiment provides a microfluidic device 1, which includes: a first chip 10 and a second chip 20 that are stacked and relatively slidable, and the first chip 10 and the second chip 20 are both made of transparent materials.

[0060] The first surface of the first chip 10 and the second surface of the second chip 20 are bonded to each other. The first surface of the first chip 10 is provided with a microchannel 11 for microbial interaction, and the second surface of the second chip 20 is provided with a plurality of pre-culture micro-grooves 21 for microbial pre-culture that are spaced apart. The first surface of the first chip 10 is also provided with a first liquid inlet channel 12 and a first liquid outlet channel 13 that can be respectively connected to the pre-culture micro-grooves 21, and the second surface of the second chip 20 is also provided with a second liquid inlet channel 22 and a second liquid outlet channel 23 that can be respectively connected to the microchannel 11.

[0061] Wherein, when the first chip 10 and the second chip 20 are in the first working position, the microchannel 11 does not overlap with the plurality of pre-culture micro-grooves 21 along the thickness direction T, and the microchannel 11 is not connected with the plurality of pre-culture micro-grooves 21; the microchannel 11 is connected with the second liquid inlet channel 22 and the second liquid outlet channel 23, and the plurality of pre-culture micro-grooves 21 are connected with the corresponding first liquid inlet channel 12 and the first liquid outlet channel 13; when the first chip 10 and the second chip 20 are in the second working position, the microchannel 11 at least partially overlaps with the plurality of pre-culture micro-grooves 21 along the thickness direction T, and the microchannel 11 is connected with the plurality of pre-culture micro-grooves 21; the microchannel 11 is disconnected from the second liquid inlet channel 22 and the second liquid outlet channel 23, and the plurality of pre-culture micro-grooves 21 are disconnected from the corresponding first liquid inlet channel 12 and the first liquid outlet channel 13. The thickness direction T refers to the thickness direction of the first chip 10 and the second chip 20. Since the first chip 10 and the second chip 20 are stacked, the thickness directions of the two are in the same direction.

[0062] In this way, by setting the stacked and relatively slidable first chip 10 and second chip 20, and the specific structure thereon, when conducting research on microbial interaction, the chip can be freely changed in position, and the overlapping time and area of ​​the pre-culture micro-groove 21 and the microchannel 11 can be regulated, thereby controlling the time and contact area of ​​microbial interaction, thereby achieving precise control of parameters. This design makes the experimental conditions of microbial interaction more flexible and controllable, and can meet different experimental needs and research purposes. Before the microorganisms enter the microchannel 11 for interaction, the connection or disconnection of the flow channel formed by the sliding chip can be used to achieve the distribution of the microorganisms in the pre-culture micro-groove 21, and then the air bath culture is carried out in the incubator. The microorganisms can grow rapidly in the pre-culture micro-groove 21, greatly shortening the experimental cycle. In addition, by setting the materials of the first chip 10 and the second chip 20 to be transparent materials, they have good light transmittance, so as to monitor the growth status, movement trajectory and interaction status of the microorganisms in real time and capture multi-dimensional information. The transparent material has a high light transmittance, and the interaction of microorganisms inside the chip can be observed from the outside in real time. That is, during the microbial cultivation process, the microbial proliferation in the pre-cultured micro-grooves 21 and the bacterial movement trajectory, quantity and morphological changes during the interaction process can be observed in real time using a microscopic imaging device. Compared with traditional methods, the microfluidic device 1 significantly improves the operability of the experiment and the richness of the data.

[0063] It should be noted that, by arranging the microchannel 11 and the corresponding second liquid inlet channel 22 and the second liquid outlet channel 23 on the first surface of the first chip 10 and the second surface of the second chip 20, that is, the microchannel 11 and the corresponding second liquid inlet channel 22 and the second liquid outlet channel 23 are respectively arranged on different planes, so that when the first chip 10 and the second chip 20 are located in the second working position, the microchannel 11 can form a closed space that is not connected to the outside world. Similarly, by arranging the pre-culture micro-groove 21 and the corresponding first liquid inlet channel 12 and the first liquid outlet channel 13 on the second surface of the second chip 20 and the first surface of the first chip 10, that is, the pre-culture micro-groove 21 and the corresponding first liquid inlet channel 12 and the first liquid outlet channel 13 are respectively arranged on different planes, so that when the first chip 10 and the second chip 20 are located in the second working position, the pre-culture micro-groove 21 can form a closed space that is not connected to the outside world. In addition, since the materials of the first chip 10 and the second chip 20 are both transparent materials, such as Figures 1 to 6 As shown, the structures on the first chip 10 and the second chip 20 and the microorganisms in the structures can be visually seen from the outside, which facilitates real-time imaging observation during the interaction of microorganisms.

[0064] In this embodiment, there are two pre-culture micro-grooves 21, which are used for pre-culture of different microorganisms respectively, but the present invention is not limited thereto. In other embodiments, different numbers of pre-culture micro-grooves 21 may be provided correspondingly according to the number of types of different microorganisms to be cultured.

[0065] In this embodiment, the microfluidic device 1 further includes: a first fixing module 30 and a second fixing module 40, the first chip 10 is fixed on the first fixing module 30, the second chip 20 is fixed on the second fixing module 40, and the materials of the first fixing module 30 and the second fixing module 40 are both transparent materials. In this way, by fixing the first chip 10 on the first fixing module 30 and the second chip 20 on the second fixing module 40, it is convenient to determine the relative position of the first chip 10 and the second chip 20, and it is convenient to drive the relative sliding of the first chip 10 and the second chip 20, and at the same time, it is possible to limit the sliding position of the first chip 10 and the second chip 20, ensuring a smooth sliding process and accurate positioning.

[0066] Specifically, the first fixing module 30 is provided with a first accommodating cavity 31, and a non-zero spacing is provided between the side wall of the first accommodating cavity 31 and the outer side wall of the first fixing module 30, and the first chip 10 is embedded in the first accommodating cavity 31; the second fixing module 40 is provided with a second accommodating cavity 41, and a non-zero spacing is provided between the side wall of the second accommodating cavity 41 and the outer side wall of the second fixing module 40, and the second chip 20 is embedded in the second accommodating cavity 41. In this embodiment, the first accommodating cavity 31 is a through hole, and similarly, the second accommodating cavity 41 is also a through hole, that is, the first accommodating cavity 31 and the second accommodating cavity 41 are both hollow spaces. However, it is not limited to this. In other embodiments, the first accommodating cavity 31 and the second accommodating cavity 41 may also be grooves. When the first accommodating cavity 31 and the second accommodating cavity 41 are grooves, since the materials of the first fixing module 30 and the second fixing module 40 are both transparent materials, that is, the bottom wall of the groove is also a transparent material, so that it is convenient for real-time imaging observation during the interaction process of microorganisms.

[0067] It should be noted that a non-zero spacing is provided between the side wall of the first accommodating cavity 31 and the outer side wall of the first fixed module 30, and the first chip 10 is embedded in the first accommodating cavity 31, that is, the outer periphery of the first chip 10 is surrounded by the physical structure of the first fixed module 30, that is, the first chip 10 is located in the middle position of the first fixed module 30; similarly, the outer periphery of the second chip 20 is surrounded by the physical structure of the second fixed module 40, that is, the second chip 20 is located in the middle position of the second fixed module 40.

[0068] Furthermore, the first surface of the first chip 10 is flush with the third surface of the first fixing module 30 facing the second fixing module 40, the second surface of the second chip 20 is flush with the fourth surface of the second fixing module 40 facing the first fixing module 30, and the third surface of the first fixing module 30 is attached to the fourth surface of the second fixing module 40. In this way, by attaching the third surface of the first fixing module 30 to the fourth surface of the second fixing module 40, a relatively closed culture environment can be provided to prevent interference from external conditions.

[0069] Preferably, along the thickness direction T, the portion of the first fixed module 30 located outside the orthographic projection of the first chip 10 is covered with a first shading layer. Along the thickness direction T, the portion of the second fixed module 40 located outside the orthographic projection of the second chip 20 is covered with a second shading layer. In this way, by providing the first shading layer and the second shading layer, the interference of external light sources on the experiment can be reduced to a certain extent, that is, the influence of external light sources on the experimental environment can be reduced to a certain extent, creating a relatively closed and stable imaging condition. In other embodiments, only the first shading layer can be provided, or only the second shading layer can be provided, which can be adjusted as needed.

[0070] The thickness of the first fixing module 30 is equal to the thickness of the first chip 10, and similarly, the thickness of the second fixing module 40 is equal to the thickness of the first chip 10. However, it is not limited thereto, and in other embodiments, the thickness of the first fixing module 30 may be greater than the thickness of the first chip 10, and / or the thickness of the second fixing module 40 may be greater than the thickness of the first chip 10. In this way, by setting the thickness of the first fixing module 30 to be greater than or equal to the thickness of the first chip 10, and / or the thickness of the second fixing module 40 to be greater than or equal to the thickness of the first chip 10, it is possible to better provide a limit for the fixed chip while having better strength.

[0071] In this embodiment, the first chip 10 and the second chip 20 are detachably connected, that is, the two can be disassembled to conveniently remove the culture matrix together with the microorganisms from the chip for subsequent separation and purification. The purified microbial samples can be further used in a variety of identification methods, including morphological observation, molecular biological analysis and related omics technologies, etc., to achieve accurate identification of microbial species and subspecies.

[0072] In this embodiment, the first chip 10 is provided with a first injection hole 14 that passes through along the thickness direction T. The first injection hole 14 is provided at one end of the first liquid inlet channel 12 away from the first liquid outlet channel 13 to facilitate the injection of liquid from the outside into the first liquid inlet channel 12. One end of the first liquid outlet channel 13 away from the first liquid inlet channel 12 is a liquid outlet end, which is provided on the side wall of the first chip 10.

[0073] The second chip 20 is provided with a second injection hole 24 that passes through along the thickness direction T. The second injection hole 24 is provided at one end of the second liquid inlet channel 22 away from the second liquid outlet channel 23 to facilitate the injection of liquid from the outside into the second liquid inlet channel 22. The second liquid outlet channel 23 is provided with a liquid outlet hole 25 at one end away from the second liquid inlet channel 22. The liquid outlet hole 25 passes through the second chip 20 along the thickness direction T.

[0074] Preferably, the depth of the first liquid inlet channel 12 and the first liquid outlet channel 13 is less than the thickness of the first chip 10, and the depth of the first liquid inlet channel 12 and the first liquid outlet channel 13 is greater than or equal to 10 microns and less than or equal to 200 microns. The depth of the second liquid inlet channel 22 and the second liquid outlet channel 23 is less than the thickness of the second chip 20, and the depth of the second liquid inlet channel 22 and the second liquid outlet channel 23 is greater than or equal to 10 microns and less than or equal to 200 microns. In this way, by setting the depth range of the first liquid inlet channel 12, the first liquid outlet channel 13, the second liquid inlet channel 22 and the second liquid outlet channel 23, it is ensured that the depth of each channel is not too deep or too shallow. This is because if it is too shallow, it will cause excessive resistance and it will be difficult to pump the liquid into the channel; if it is too deep, there will be a risk that the liquid is not easy to fill the channel, resulting in the inability to control the dosage, which affects the experimental results.

[0075] Preferably, the microchannel 11 and the pre-culture micro-groove 21 have different sizes.

[0076] In this embodiment, the microfluidic device 1 further includes a lubricating layer, which is disposed between the first surface of the first chip 10 and the second surface of the second chip 20. Thus, by disposing the lubricating layer between the first surface of the first chip 10 and the second surface of the second chip 20, the friction resistance during the relative sliding between the first chip 10 and the second chip 20 can be effectively reduced, so that the conversion between the first working position and the second working position of the first chip 10 and the second chip 20 is smoother; at the same time, disposing the lubricating layer between the first surface of the first chip 10 and the second surface of the second chip 20 can also prevent external interference during the culture process. Preferably, the thickness of the lubricating layer is less than or equal to 10 microns.

[0077] Preferably, the culture matrix in the microchannel 11 is a photosensitive or thermosensitive material, and the culture matrix is ​​solidified by temperature or light of a specific wavelength. In this way, after the microorganisms interact, the culture matrix can be solidified, the spatial position information of the bacterial interaction can be retained, and the separation and identification of microbial subspecies can be achieved based on relevant omics technology. This solidification process can not only record and preserve the dynamic process of microbial interaction, but also provide a stable physical platform for subsequent analysis and research.

[0078] like Figure 5 and Figure 6 As shown, the working process of this embodiment is as follows:

[0079] First, the first chip 10 and the second chip 20 are assembled so that the first chip 10 and the second chip 20 are located in the first working position. At this time, the microchannel 11 and the multiple pre-culture micro-grooves 21 do not overlap along the thickness direction T, and the microchannel 11 is not connected to the multiple pre-culture micro-grooves 21; the microchannel 11 is connected to the second liquid inlet channel 22 and the second liquid outlet channel 23, and the multiple pre-culture micro-grooves 21 are connected to the corresponding first liquid inlet channel 12 and the first liquid outlet channel 13. Different microorganisms are injected into the corresponding first liquid inlet channel 12 in sequence, and pre-amplified in the corresponding pre-culture micro-grooves 21. When the microorganism enters the logarithmic growth phase, the culture matrix is ​​injected into the microchannel 11 through the second liquid inlet channel 22 to provide nutrients for subsequent interactions in the microchannel 11.

[0080] Next, the first chip 10 and the second chip 20 are placed in the second working position. At this time, the microchannel 11 and the multiple pre-cultured micro-grooves 21 are at least partially overlapped along the thickness direction T, and the microchannel 11 is connected to the multiple pre-cultured micro-grooves 21; the microchannel 11 is disconnected from the second liquid inlet channel 22 and the second liquid outlet channel 23, and the multiple pre-cultured micro-grooves 21 are disconnected from the corresponding first liquid inlet channel 12 and the first liquid outlet channel 13. Different microorganisms enter the microchannel 11 for observing the interaction from the corresponding pre-cultured microgrooves 21. The microorganisms further proliferate in the microchannel 11 and begin to influence each other. Different microorganisms show different movement trajectories, morphologies and interaction results. The interaction of different microorganisms is monitored in real time through the microscopic imaging module (including movement trajectories, quantity, morphology, etc., and multi-dimensional interaction information is collected). Specifically, a concentration gradient can be generated by diffusion in the microchannel 11 to construct a chemotaxis model of microorganisms.

[0081] Finally, the microorganisms are fixed in space by solidifying the culture matrix, and the culture matrix containing the microorganisms is taken out by disassembling the first chip 10 and the second chip 20, and the spatial position information is recorded, and the results of the microbial interaction are analyzed by combining multi-omics analysis methods. That is, the spatial distribution information of the microorganisms is obtained by using the solidification characteristics of the culture matrix, and the interaction mechanism is analyzed by combining subsequent omics methods.

[0082] This embodiment also provides a microfluidic device, which includes the microfluidic device 1 as described above.

[0083] Although the specific implementations of the utility model are described above, those skilled in the art should understand that this is only an example, and the protection scope of the utility model is defined by the attached claims. Those skilled in the art can make various changes or modifications to these implementations without departing from the principle and essence of the utility model, but these changes and modifications fall within the protection scope of the utility model.

Claims

1. A microfluidic device, characterized in that: It includes: A first chip and a second chip are stacked and slidably arranged relative to each other, wherein the first chip and the second chip are both made of transparent material; The first surface of the first chip and the second surface of the second chip are bonded to each other, the first surface of the first chip is provided with a microchannel for microbial interaction, and the second surface of the second chip is provided with a plurality of pre-culture micro-grooves for pre-culture of microorganisms arranged at intervals; the first surface of the first chip is also provided with a first liquid inlet channel and a first liquid outlet channel respectively connected to the pre-culture micro-grooves, and the second surface of the second chip is also provided with a second liquid inlet channel and a second liquid outlet channel respectively connected to the microchannel; Wherein, when the first chip and the second chip are in the first working position, the microchannel and the plurality of pre-culture micro-grooves do not overlap in the thickness direction, and the microchannel and the plurality of pre-culture micro-grooves are not connected; the microchannel is connected with the second liquid inlet channel and the second liquid outlet channel, and the plurality of pre-culture micro-grooves are connected with the corresponding first liquid inlet channel and first liquid outlet channel; When the first chip and the second chip are in the second working position, the microchannel at least partially overlaps with the plurality of pre-culture micro-grooves along the thickness direction, and the microchannel is connected to the plurality of pre-culture micro-grooves; the microchannel is disconnected from the second liquid inlet channel and the second liquid outlet channel, and the plurality of pre-culture micro-grooves are disconnected from the corresponding first liquid inlet channel and first liquid outlet channel.

2. The microfluidic device according to claim 1, characterized in that The microfluidic device further includes: a first fixing module and a second fixing module, the first chip is fixed on the first fixing module, the second chip is fixed on the second fixing module, and the first fixing module and the second fixing module are both made of transparent materials.

3. The microfluidic device according to claim 2, characterized in that The first fixing module is provided with a first accommodating cavity, a non-zero spacing is provided between the side wall of the first accommodating cavity and the outer side wall of the first fixing module, the first chip is embedded in the first accommodating cavity, and the first accommodating cavity is a groove or a through hole; and / or, The second fixing module is provided with a second accommodating cavity, a non-zero spacing is provided between the side wall of the second accommodating cavity and the outer side wall of the second fixing module, the second chip is embedded in the second accommodating cavity, and the second accommodating cavity is a groove or a through hole.

4. The microfluidic device according to claim 2, characterized in that: Along the thickness direction, a portion of the first fixing module located outside the orthographic projection of the first chip is covered with a first light shielding layer; and / or, Along the thickness direction, a portion of the second fixing module located outside the orthographic projection of the second chip is covered with a second light shielding layer.

5. The microfluidic device according to claim 2, characterized in that: The thickness of the first fixing module is greater than or equal to the thickness of the first chip; and / or, The thickness of the second fixing module is greater than or equal to the thickness of the first chip.

6. The microfluidic device according to claim 1, characterized in that: The first chip is provided with a first liquid injection hole penetrating along the thickness direction, and the first liquid injection hole is arranged at an end of the first liquid inlet channel away from the first liquid outlet channel; and / or, The second chip is provided with a second liquid injection hole penetrating along the thickness direction, and the second liquid injection hole is arranged at an end of the second liquid inlet channel away from the second liquid outlet channel.

7. The microfluidic device according to claim 1, characterized in that: The depths of the first liquid inlet channel and the first liquid outlet channel are both less than the thickness of the first chip, and the depths of the first liquid inlet channel and the first liquid outlet channel are both greater than or equal to 10 microns and less than or equal to 200 microns; and / or, The depths of the second liquid inlet channel and the second liquid outlet channel are both less than the thickness of the second chip, and the depths of the second liquid inlet channel and the second liquid outlet channel are both greater than or equal to 10 microns and less than or equal to 200 microns.

8. The microfluidic device according to any one of claims 1 to 7, characterized in that: The microfluidic device further includes a lubricating layer, which is disposed between the first surface of the first chip and the second surface of the second chip.

9. The microfluidic device according to claim 8, characterized in that The thickness of the lubricating layer is less than or equal to 10 microns.

10. A microfluidic device, characterized in that: It comprises the microfluidic device according to any one of claims 1 to 9.

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