A batch filling and using device for separating and purifying total saponin chromatography column in laboratory

Abstract

The utility model relates to the technical field of purification devices, and in particular to a batch filling and use device for separating and purifying total saponin chromatography columns for laboratories. Its technical solution includes: a chromatography column tube, a three-way shutoff joint and a liquid inlet hose, a filler sieve plate is arranged inside the chromatography column tube, a three-way shutoff joint is plugged and installed on the outer wall of the lower end of the chromatography column tube, a solid phase extractor is arranged on the outer wall of the lower end of the chromatography column tube, and a lower end interface is arranged on the outer wall of the lower end of the three-way shutoff joint. By arranging the chromatography column tube, the three-way shutoff joint and the solid phase extractor, the effect of improving the separation efficiency is achieved, the efficiency of separating and purifying large quantities of samples is increased, and the accuracy of the test results is improved. At the same time, because the material is changed to PVC, the column tube can be reused, which overcomes the defects of heavy weight and easy damage of glass chromatography tubes, and saves costs.

Description

Technical Field

[0001] The utility model relates to the technical field of purification devices, in particular to a batch filling and using device for a laboratory separation and purification total saponin chromatography column. Background Art

[0002] Total saponin generally refers to ginsenosides, a natural plant compound, clinically known as saponin, also known as alkali saponin, saponin, saponin or saponin. Saponin (Saponin) is a type of glycoside with triterpenoid or spirosterane compounds as aglycones, mainly distributed in higher terrestrial plants, and also exists in small amounts in marine organisms such as starfish and sea cucumbers. The main active ingredients of many Chinese herbal medicines such as ginseng, polygala, platycodon, licorice, rhizoma anemarrhenae and bupleurum contain saponins. The extraction, separation and purification of total saponins in the standard method all use a 10mL syringe as a chromatography tube, filled with 3cm Amberlite-XAD-2 macroporous resin, and 1cm neutral alumina with a particle size of 100-200 mesh.

[0003] The prior art has many benefits during use, but still has the following problems. Although this type of chromatographic column seems simple, the macroporous resin and neutral alumina particles are easy to stick to the wall during the actual dry filling operation, and it is necessary to knock repeatedly to make the column bed uniform and compact. And because the adsorption heat release between the "walking column" solvent and the filler is easy to produce bubbles, the quality of the chromatographic column bed is difficult to guarantee. If the bubbles in the column bed are not found, the bubbles will be released during the operations such as loading and adding solvent, causing the column to become flowery, and the sample is difficult to pass smoothly, so that the separation effect cannot be achieved, and the accuracy of the experimental results cannot be guaranteed. In addition, since the outlet of the lower end of the syringe is generally plugged with absorbent cotton as a baffle, the macroporous resin debris, sample liquid residue and particulate matter will gather and block the outlet of the syringe during actual operation, causing the experiment to be interrupted or abandoned, thereby affecting the experimental process and reducing work efficiency. At the same time, this method can only be filled or used one by one at present, and cannot be prepared in batches. Therefore, when the sample amount is large, it cannot meet the batch filling and use, and the efficiency is greatly limited. Utility Model Content

[0004] The utility model aims to provide a batch filling and using device for a laboratory separation and purification total saponin chromatography column to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a batch filling of a chromatography column for separating and purifying total saponins in a laboratory, comprising a chromatography column tube, a three-way shut-off joint and a liquid inlet hose, wherein a filler sieve plate is arranged inside the chromatography column tube, a three-way shut-off joint is inserted and installed on the outer wall of the lower end of the chromatography column tube, a solid phase extractor is arranged on the outer wall of the lower end of the chromatography column tube, and a lower end interface is arranged on the outer wall of the lower end of the three-way shut-off joint.

[0006] When using a batch filling and using device for separating and purifying total saponin chromatography columns for laboratory use in the technical solution, first install a filler sieve plate at the lower end of the chromatography column tube, then insert and fix them one by one on the extraction column bracket, and at the same time convert the support interface and the upper end interface of the three-way shut-off joint, keep the lower end interface closed inside, cover the upper port of the chromatography column tube with a screw cap, and then connect the multi-channel liquid separation constant flow pump and the liquid outlet hose in sequence. At the same time, each three-way shut-off joint and the branch interface are connected to the multi-channel liquid separation constant flow pump and the liquid inlet hose, and Amberlite-XAD-2 macroporous resin and neutral alumina are soaked in 20% ethanol to obtain two kinds of filling glue suspensions, and the multi-channel liquid separation constant flow pump is filled with Amberlite-XAD-2 macroporous resin in batches, and filled to their respective scales according to the type of chromatography column tube, and the flow rate is increased and pressurized until the column bed liquid level no longer drops, and then the column bed is filled with neutral alumina ethanol glue suspension to their respective scales. , increase the flow rate and pressurize until the column bed liquid level no longer drops, and then continue to compact the column bed at a moderate flow rate for 1 hour. According to the type of chromatography column tube, select another suitable sieve plate and fix it on the neutral alumina column bed to stabilize the horizontal surface of the column bed. Turn off the power supply of the multi-channel liquid separation constant flow pump, switch the position of the three-way shut-off joint to close each branch, disconnect each connecting pipe of the multi-channel liquid separation constant flow pump and cover it with a dust cover. When the chromatography column tube is prepared in batches and needs to be used, replace the column tube screw cover with a column tube adapter, insert it into the upper end of the chromatography column tube and use a syringe as a eluent and eluent holder. When adding liquid to elute, load and elute the column bed, switch the position of the three-way shut-off joint to close the branch interface and connect the upper and lower interface outlets in series. If the elution, loading and elution speeds are too slow, the user can flexibly adjust the vacuum pump pressure to control the liquid outflow speed as needed, thereby selecting a suitable chromatography column tube to cooperate with the above-mentioned device to prepare and use the total saponin separation and purification chromatography column in batches.

[0007] Preferably, an upper end interface is provided on one side of the outer wall of the upper end of the three-way shut-off joint, and the upper end interface is connected to the column tube of the chromatography column.

[0008] Preferably, a branch interface is provided on one side outer wall of the three-way cut-off joint, a liquid outlet hose is installed on one side outer wall of the branch interface, a multi-channel liquid separation constant flow pump is provided on one side outer wall of the liquid outlet hose, and liquid inlet hoses equidistantly distributed in parallel are installed on one side outer wall of the multi-channel liquid separation constant flow pump. The multi-channel liquid separation constant flow pump is used to pump liquid.

[0009] Preferably, the upper outer wall of the chromatography column tube is provided with a screw cap, the liquid inlet hose is connected to the screw cap, and the outer wall of one side of the chromatography column tube is provided with a dust cover. The screw cap can connect the liquid inlet hose to the chromatography column tube, and the dust cover can shield the chromatography column tube.

[0010] A device for using a chromatography column for separating and purifying total saponins in a laboratory comprises a chromatography column tube and a solid phase extractor, wherein the solid phase extractor is arranged on the outer wall of the lower end of the chromatography column tube, a vacuum pump is arranged on the outer wall of one end of the solid phase extractor, and extraction column brackets which are equidistantly and parallelly distributed are installed on the outer wall of the upper end of the solid phase extractor.

[0011] Preferably, a column tube transfer interface is provided on the outer wall of the upper end of the column tube of the chromatography column, and a syringe is provided on the outer wall of the upper end of the column tube transfer interface.

[0012] Compared with the prior art, the utility model has the following beneficial effects: the utility model achieves the effect of improving separation efficiency by arranging a chromatography column tube, a three-way shut-off joint and a solid phase extractor, thereby increasing the efficiency of separation and purification of large quantities of samples and improving the accuracy of the detection results; at the same time, since the material is replaced with PVC, the column tube can be reused, thus overcoming the defects of heavy weight and easy breakage of glass chromatography tubes and saving costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic diagram of the chromatography column tube structure of the utility model;

[0014] Figure 2 This is a schematic diagram of the structure of the syringe of the utility model;

[0015] Figure 3 This is a schematic diagram of the column-tube transfer interface structure of the utility model;

[0016] Figure 4 It is a schematic diagram of the structure of a three-way shut-off joint of the utility model.

[0017] In the figure: 1. chromatography column tube; 2. packing sieve plate; 3. three-way shut-off joint; 3-1. upper end interface; 3-2. branch interface; 3-3. lower end interface; 4. dust cover; 5. screw cover; 6. multi-channel liquid separation constant flow pump; 7. solid phase extractor; 7-1. extraction column bracket; 7-2. vacuum pump; 8. liquid outlet hose; 9. liquid inlet hose; 10. column tube adapter; 11. syringe. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0019] See also Figure 1-Figure 4 , the utility model provides two embodiments:

[0020] Embodiment 1: A batch filling and using device for separating and purifying total saponin chromatography columns for laboratory use, comprising a chromatography column tube 1, a three-way shutoff joint 3 and a liquid inlet hose 9, wherein a filler sieve plate 2 is arranged inside the chromatography column tube 1, and the chromatography column tube 1 adopts the currently popular solid phase extraction small column as a model, and adopts a PVC material with a graduated syringe-shaped affinity column empty column (12mL, column height 79.2mm, inner diameter 15.6mm / 20ML, column height 93.6mm, inner diameter 19. 4mm / 30mL, column height 105.7mm, inner diameter 23.5mm), the filler sieve plate 2 is a finished 12mL, 20mL and 30mL column sieve plate with a thickness of 1.5mm and a pore size of 10μm made of PE. At the upper and lower ends of the chromatography column tube, a three-way shut-off joint 3 is installed on the outer wall of the lower end of the chromatography column tube 1, a solid phase extractor 7 is arranged on the outer wall of the lower end of the chromatography column tube 1, and a lower end interface 3-3 is arranged on the outer wall of the lower end of the three-way shut-off joint 3.

[0021] An upper end interface 3 - 1 is provided on one side of the outer wall of the upper end of the three-way shut-off joint 3 , and the upper end interface 3 - 1 is connected to the chromatography column tube 1 .

[0022] A branch interface 3-2 is provided on one side of the three-way cut-off joint 3, a liquid outlet hose 8 is installed on one side of the branch interface 3-2, a multi-channel liquid separation constant flow pump 6 is provided on one side of the liquid outlet hose 8, and an equidistant and parallel liquid inlet hose 9 is installed on one side of the multi-channel liquid separation constant flow pump 6. The multi-channel liquid separation constant flow pump 6 is used to pump liquid.

[0023] The upper outer wall of the chromatography column tube 1 is provided with a screw cap 5, and the liquid inlet hose 9 is connected to the screw cap 5. The outer wall of one side of the chromatography column tube 1 is provided with a dust cover 4. The screw cap 5 can connect the liquid inlet hose 9 to the chromatography column tube 1, and the dust cover 4 can shield the chromatography column tube 1. First, a filler sieve plate 2 is installed at the lower end of the chromatography column tube 1, and then inserted and fixed one by one on the extraction column bracket 7-1, and at the same time, the support interface and the upper end interface 3-1 of the three-way shutoff joint 3 are converted, and the lower end interface 3-3 is kept closed inside. After the upper end of the chromatography column tube 1 is covered with a screw cap 5, the multi-channel liquid separation constant flow pump 6 and the liquid outlet hose 8 are connected in sequence. At the same time, each three-way shutoff joint 3 and the branch interface 3-2 are connected to the multi-channel liquid separation constant flow pump 6 and the liquid inlet hose 9, and two kinds of filling glue are obtained by soaking Amberlite-XAD-2 macroporous resin and neutral alumina in 20% ethanol respectively. The concentration of the suspension is controlled at 50%-60%. The multi-channel liquid separation constant flow pump 6 is filled with Amberlite-XAD-2 macroporous resin in batches, and is filled to respective scales according to the type of the chromatography column tube 1. The flow rate is increased and pressurized until the column bed liquid level no longer drops, and then the neutral alumina ethanol gel suspension is used to fill the column bed to respective scales. The flow rate is increased and pressurized until the column bed liquid level no longer drops, and then the column bed is compacted at a moderate flow rate for 1 hour. Another suitable sieve plate is selected according to the type of the chromatography column tube 1, fixed on the neutral alumina column bed to stabilize the horizontal surface of the column bed, the power supply of the multi-channel liquid separation constant flow pump 6 is turned off, the position of the three-way shut-off joint 3 is switched to close each branch, and the dust cover 4 is covered after the connecting pipes of the multi-channel liquid separation constant flow pump 6 are disconnected.

[0024] Embodiment 2: A batch filling and use device for separating and purifying total saponin chromatography columns for laboratory use, comprising a chromatography column tube 1, a three-way shut-off joint 3 and a liquid inlet hose 9, a filler sieve plate 2 is arranged inside the chromatography column tube 1, a three-way shut-off joint 3 is inserted and installed on the outer wall of the lower end of the chromatography column tube 1, a solid phase extractor 7 is arranged on the outer wall of the lower end of the chromatography column tube 1, and a lower end interface 3-3 is arranged on the outer wall of the lower end of the three-way shut-off joint 3.

[0025] An upper end interface 3 - 1 is provided on one side of the outer wall of the upper end of the three-way shut-off joint 3 , and the upper end interface 3 - 1 is connected to the chromatography column tube 1 .

[0026] A branch interface 3-2 is provided on one side of the three-way cut-off joint 3, a liquid outlet hose 8 is installed on one side of the branch interface 3-2, a multi-channel liquid separation constant flow pump 6 is provided on one side of the liquid outlet hose 8, and an equidistant and parallel liquid inlet hose 9 is installed on one side of the multi-channel liquid separation constant flow pump 6. The multi-channel liquid separation constant flow pump 6 is used to pump liquid.

[0027] The upper outer wall of the chromatography column tube 1 is provided with a screw cap 5, the liquid inlet hose 9 is connected to the screw cap 5, and the outer wall of one side of the chromatography column tube 1 is provided with a dust cover 4. The screw cap 5 can connect the liquid inlet hose 9 to the chromatography column tube 1, and the dust cover 4 can shield the chromatography column tube 1.

[0028] A device for using a chromatography column for separating and purifying total saponins in a laboratory comprises a chromatography column tube 1 and a solid phase extractor 7. The solid phase extractor 7 is arranged on the outer wall of the lower end of the chromatography column tube 1, a vacuum pump 7-2 is arranged on the outer wall of one end of the solid phase extractor 7, and extraction column brackets 7-1 are installed on the outer wall of the upper end of the solid phase extractor 7 in parallel with each other.

[0029] The upper outer wall of the column tube 1 of the chromatography column is provided with a column tube transfer interface 10, and the upper outer wall of the column tube transfer interface 10 is provided with a syringe 11. First, a filler sieve plate 2 is installed at the lower end of the column tube 1 of the chromatography column, and then inserted and fixed one by one on the extraction column bracket 7-1, and at the same time, the support interface and the upper interface 3-1 of the three-way shutoff joint 3 are converted, and the lower end interface 3-3 is kept closed. After the upper port of the column tube 1 is covered with a screw cap 5, the multi-channel liquid separation constant flow pump 6 and the liquid outlet hose 8 are connected in sequence. At the same time, each three-way shutoff joint 3, the branch interface 3-2 and the multi-channel liquid separation constant flow pump 6, the liquid inlet The hose 9 is connected, and the Amberlite-XAD-2 macroporous resin and the neutral alumina are soaked in 20% ethanol to obtain two kinds of filling glue suspensions. The multi-channel liquid separation constant flow pump 6 is filled with the Amberlite-XAD-2 macroporous resin in batches, and is filled to the respective scales according to the type of the chromatography column tube 1 (the scale is 5ml, 10ml or 15ml, and the specific scale value is calculated according to the required amount of filler), and the flow rate is increased and the pressure is increased until the column bed liquid level does not drop, and then the neutral alumina ethanol glue suspension is used to fill the column bed to the respective scales (the scale is 7ml, 12mL or 17mL, the specific scale value is calculated according to the required amount of filler), increase the flow rate and pressurize until the column bed liquid level no longer drops, and then continue to compact the column bed at a moderate flow rate for 1h. According to the type of chromatography column tube 1, select another suitable sieve plate and fix it on the neutral alumina column bed to stabilize the horizontal surface of the column bed. Turn off the power of the multi-channel liquid separation constant flow pump 6, switch the position of the three-way shut-off joint 3 to close each branch, disconnect the multi-channel liquid separation constant flow pump 6 and cover the dust cover 4 after each connecting pipe. When the chromatography column tube 1 is prepared in batches and needs to be used, replace the column tube screw cover 5 with the column tube adapter 10. After inserting the upper end of the chromatography column tube 1, the syringe 11 is used as the eluent and elution container. The syringe 11 is designed to be 30ml. When adding liquid for elution, sample loading and elution of the column bed, the position of the three-way interception joint 3 is switched to a state in which the branch interface 3-2 is closed and the upper end interface 3-1 and the lower end interface 3-3 are connected in series. If the elution, sample loading and elution speeds are too slow, the user can flexibly adjust the pressure of the vacuum pump 7-2 to control the liquid outflow speed as needed, thereby selecting a suitable chromatography column tube and using it in conjunction with the above-mentioned device to prepare and use a total saponin separation and purification chromatography column in batches.

[0030] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A batch filling method of a chromatography column for separating and purifying total saponins in a laboratory, comprising a chromatography column tube (1), a three-way shutoff joint (3) and a liquid inlet hose (9), characterized in that: A filler sieve plate (2) is arranged inside the chromatography column tube (1), a three-way shutoff joint (3) is inserted and installed on the outer wall of the lower end of the chromatography column tube (1), a solid phase extractor (7) is arranged on the outer wall of the lower end of the chromatography column tube (1), and a lower end interface (3-3) is arranged on the outer wall of the lower end of the three-way shutoff joint (3).

2. The batch filling of a chromatography column for separating and purifying total saponins for laboratory use according to claim 1, characterized in that: An upper end interface (3-1) is provided on one side of the upper end outer wall of the three-way shut-off joint (3), and the upper end interface (3-1) is connected to the chromatography column tube (1).

3. The batch filling of a chromatography column for separating and purifying total saponins for laboratory use according to claim 1, characterized in that: A branch interface (3-2) is provided on one side outer wall of the three-way shut-off joint (3), a liquid outlet hose (8) is plugged and installed on one side outer wall of the branch interface (3-2), a multi-channel liquid separation constant flow pump (6) is provided on one side outer wall of the liquid outlet hose (8), and liquid inlet hoses (9) that are equidistantly distributed in parallel are plugged and installed on one side outer wall of the multi-channel liquid separation constant flow pump (6).

4. The batch filling of a chromatography column for separating and purifying total saponins for laboratory use according to claim 1, characterized in that: The upper outer wall of the chromatography column tube (1) is provided with a screw cover (5), the liquid inlet hose (9) is connected to the screw cover (5), and the outer wall of one side of the chromatography column tube (1) is provided with a dust cover (4).

5. A laboratory device for separating and purifying total saponins chromatography column, comprising a chromatography column tube (1) and a solid phase extractor (7), characterized in that: The outer wall of the lower end of the chromatography column tube (1) is provided with a solid phase extractor (7), the outer wall of one end of the solid phase extractor (7) is provided with a vacuum pump (7-2), and the outer wall of the upper end of the solid phase extractor (7) is installed with extraction column brackets (7-1) that are equidistantly distributed in parallel.

6. The device for using the laboratory separation and purification total saponin chromatography column according to claim 5, characterized in that: The upper outer wall of the chromatography column tube (1) is provided with a column tube transfer interface (10), and the upper outer wall of the column tube transfer interface (10) is provided with a syringe (11).

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